Aquaculture Reports最新文献

{"title":"Oral administration of anti-lipopolysaccharide factor isoform 3 via the bait microalga for controlling bacterial infection of Atlantic Bay scallop (Argopecten irradians)","authors":"Yulin Cui ,&nbsp;Ping Liu ,&nbsp;Yaping Shao ,&nbsp;Fengjie Sun ,&nbsp;Qiaolei Wang ,&nbsp;Hao Zhang ,&nbsp;Yan Cheng ,&nbsp;Zhengquan Gao","doi":"10.1016/j.aqrep.2024.102430","DOIUrl":"10.1016/j.aqrep.2024.102430","url":null,"abstract":"<div><div>In order to reduce antibiotic application in aquaculture, an antimicrobial peptide anti-lipopolysaccharide factor isoform 3 (ALFPm3) with highly effective inhibitory activity against pathogens was introduced to bay scallop (<em>Argopecten irradians</em>) using bait microalga <em>Tetraselmis subcordiformis</em> as a delivery system. To improve its expression and stability, a fusion of two <em>alfpm3</em> genes (<em>alfpm3–2</em>) was inserted into an expression vector and transformed into <em>T. subcordiformis</em> chloroplast, followed by <em>in vitro</em> and <em>in vivo</em> functional evaluations of the antimicrobial activities of ALFPm3–2 by minimum inhibitory concentration method and bay scallop breeding experiment, respectively. The results showed that the transformants of <em>T. subcordiformis</em> and the purified ALFPm3–2 had significant inhibitory effects on <em>Vibrio parahaemolyticus</em> and <em>Staphylococcus aureus in vitro</em>, while feeding the transformants reduced the colonization of the two pathogens in bay scallops, which further reduced bay scallops’ mortality by 28.67 % as revealed in infection test. Furthermore, daily oral administration of ALFPm3–2 could improve the gut microbial community and reduce the inflammatory response. Combined with changes in immune factors and endotoxin, it indicated that exogenous ALFPm3–2 played antimicrobial and anti-inflammatory functions in the guts of bay scallops. Overall, this study provided strong evidence to support the oral administration of antimicrobial peptides via microalgae in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102430"},"PeriodicalIF":3.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of silkworm pupae powder on growth performance, muscle fatty acid composition, and intestinal function in mandarin fish (Siniperca chuatsi)","authors":"Qingrong Li ,&nbsp;Bing Fu ,&nbsp;Long Huang ,&nbsp;Fubao Wang ,&nbsp;Donglai Zhou ,&nbsp;Qiong Yang ,&nbsp;Yuxiao Zou ,&nbsp;Yang Xiao ,&nbsp;Sentai Liao ,&nbsp;Dongxu Xing","doi":"10.1016/j.aqrep.2024.102435","DOIUrl":"10.1016/j.aqrep.2024.102435","url":null,"abstract":"<div><div>This study examined the effects of dietary silkworm pupae powder (SWP) supplementation on the growth performance, muscle fatty acid composition, and intestinal function in mandarin fish (<em>Siniperca chuatsi</em>) during a 60-day feeding trial. A total of 360 fish (initial body weight 52.44 ± 0.06 g) were distributed randomly into three groups with three replicates per group and 40 fish per replicate. The three groups were fed either the basal diet, supplementation with 1.0 % fermented SWP (T1), or supplementation with 1.0 % fermented induced SWP (T2, induced by <em>Beauveria bassiana</em>). Results showed that fish fed T1 had significantly higher (<em>P</em> &lt; 0.05) condition factor, C16:0, C20:4n-6, C20:5 n3, C22:6n3 C22:5n6, C22:5n-3, and n-3/n-6 levels, but lower (<em>P</em> &lt; 0.05) fat body ratio, C18:1, C18:2n-6 and crude lipid levels than those fish fed basal diet. All fish had similar (<em>P</em> &gt; 0.05) weight gain rate, specific growth rate, feed conversion rate, feed intake, and hepatosomatic index. Fish fed T2 had higher (<em>P</em> &lt; 0.05) trypsin activity than those fish fed basal diet. Compared with the basal diet, fish fed T1 or T2 had higher (<em>P</em> &lt; 0.05) villus length, muscular layer thickness, glutathione peroxidase, alkaline phosphatase, and lysozyme activities, but lower (<em>P</em> &lt; 0.05) triglyceride and total cholesterol levels. Comparative transcriptomic analysis indicated that T1 or T2 enhanced immunity and lipid metabolism ability, as manifested in the up-regulated expression of <em>il12rb1</em>, <em>scdb</em>, <em>tlr2,</em> and <em>FABP7</em>, and the down-regulated expression of <em>EBP</em> and <em>hsd17b7</em>. These findings suggest that dietary supplementation with fermented SWP could enhance the health and quality of mandarin fish, potentially offering a valuable strategy for improving aquaculture productivity.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102435"},"PeriodicalIF":3.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eucommia ulmoides Oliv. leaf extract: Effects on growth, antioxidant capacity, and lipid metabolism in Red swamp crayfish (Procambarus clarkii)","authors":"Dan Yang ,&nbsp;Wenbo Sun ,&nbsp;Mengdan Hou ,&nbsp;Chuanbo Xiao ,&nbsp;Honghao Jin ,&nbsp;Yong Lin ,&nbsp;Dapeng Wang ,&nbsp;Hua Ye ,&nbsp;Hui Luo","doi":"10.1016/j.aqrep.2024.102425","DOIUrl":"10.1016/j.aqrep.2024.102425","url":null,"abstract":"<div><div>The addition of <em>Eucommia ulmoides</em> Oliv. leaf extract (ELE) enhances growth, immunity and antioxidant capacity in crustaceans, which has not been studied on the red swamp crayfish (<em>Procambarus clarkii</em>). Hence, this study aimed to investigate the effect of ELE on the growth, antioxidant capacity, digestive enzymes activities, and lipid metabolism of red swamp crayfish. A total of 240 individuals of red swamp crayfish were randomly assigned to four groups. Each group was fed with feed a diet supplemented with varying doses of ELE: 0 g/kg (CK), 0.5 g/kg (T2), 1.0 g/kg (T3), and 1.5 g/kg (T4). After 30 days, the results indicated that T2 group exhibited a significantly higher final average weight, weight gain rate, and specific growth rate compared to the CK (<em>P</em>&lt;0.05). Significantly lower feed conversion ratios than the CK (<em>P</em>&lt;0.05) were observed in the T2 and T3 groups. For antioxidant indicators, hepatopancreas superoxide dismutase (SOD) activity was observed to significantly increased in the T3 and T4 groups, while levels of malondialdehyde (MDA) in which were significantly reduced in hepatopancreas and hemolymph (<em>P</em>&lt;0.05). Regarding biochemical indicators and intestinal function, significant enhancement of lipase (LPS) activity was observed in the T2 group, which decreased in the T4, compared to the CK (<em>P</em>&lt;0.05). Moreover, significant decrease of the glucose (GLU), total triglyceride (TG), and total cholesterol (TC) content of the hemolymph (<em>P</em>&lt;0.05) were observed in the T4 group. Transcriptome analysis revealed that differentially expressed genes in the T4 group were primarily enriched in peroxisome proliferator-activated receptor (PPAR), and lipid metabolism pathways. Furthermore, it seems that ELE supplements significantly upregulated the expression of <em>apoa-II</em>, <em>acaox1</em>, <em>acox3</em>, <em>me1</em>, and <em>plin1</em> in the PPAR signaling pathway to regulate lipid metabolism. In conclusion, 0.5 g/kg and 1.5 g/kg ELL addition amount can enhance growth performance, antioxidant capacity, and lipid metabolism of red swamp crayfish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102425"},"PeriodicalIF":3.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary lipid levels on antioxidant capacity, inflammatory response and endoplasmic reticulum stress in immune organ of turbot (Scophthalmus maximus L.)","authors":"Dan Xu ,&nbsp;Yingming Yang ,&nbsp;Junrong Feng ,&nbsp;Yongzheng Tang ,&nbsp;Pei Yang ,&nbsp;Fangrui Lou","doi":"10.1016/j.aqrep.2024.102434","DOIUrl":"10.1016/j.aqrep.2024.102434","url":null,"abstract":"<div><div>Lipid, one of the most important macronutrients of organism, can provide energy and essential fatty acids for aquatic animals. Excessive lipid level in diet has negative effects on fish health. However, the regulation mechanism of lipid level on the immunity of marine fish is still not completely understood. Therefore, a 56-day feeding trial was conducted to evaluate the effects of dietary lipid levels on antioxidant capacity, inflammatory response and endoplasmic reticulum stress in immune organs of turbot (<em>Scophthalmus maximus</em> L.). Turbot (9.87 ± 0.13 g) were fed a diet with a low (lipid 6 %, L6), medium (lipid 12 %, L12) and high (lipid 18 %, L18) crude lipid content. Results showed that the L18 group significantly reduced the survival rate during the <em>V. harveyi</em> challenge test compared with L6 and L12 groups. The respiratory burst activity, lysozyme (LZM) and alkaline phosphatase (AKP) in serum were significantly decreased in L6 and L18 groups. Moreover, the L18 group significantly decreased the T-AOC, superoxide dismutase (SOD) and catalase (CAT) activities in head kidney and spleen than that in the L12 group, while the malondialdehyde (MDA) content had no significant changes. Furthermore, the L18 group significantly upregulated the pro-inflammatory genes (<em>tnf-α</em>, <em>il-1β</em>, <em>ifn-γ</em>, <em>p65</em> and <em>caspase7</em>) expression and the phosphorylation level of the c-Jun N-terminal kinase (JNK) - Mitogen-activated protein kinase (MAPK) pathway in head kidney. Similarly, in spleen, the pro-inflammatory genes (<em>il-1β</em>, <em>ifn-γ</em>, <em>p65</em> and <em>caspase7</em>) expression were also significantly upregulated in the L18 group. In addition, the endoplasmic reticulum (ER) stress related genes and proteins expression in head kidney and spleen were also significantly upregulated in the L18 group. Overall, these results revealed that the high lipid level in diet suppressed antioxidant capacity and induced inflammatory response of turbot. This study could advance the understanding of effects of dietary lipid levels on turbot (<em>Scophthalmus maximus</em> L<em>.</em>) and improve the utilization rate of high-fat diets in aquaculture<em>.</em></div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102434"},"PeriodicalIF":3.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing growth, immunity, and gene expression in Nile Tilapia (Oreochromis niloticus) through dietary supplementation with avocado (Persea americana) seed powder","authors":"Chinh Le Xuan ,&nbsp;Nguyen Vu Linh ,&nbsp;Supreya Wannavijit ,&nbsp;Piyatida Outama ,&nbsp;Anisa Rilla Lubis ,&nbsp;Vimbai Irene Machimbirike ,&nbsp;Yupa Chromkaew ,&nbsp;Yuthana Phimolsiripol ,&nbsp;Hien Van Doan","doi":"10.1016/j.aqrep.2024.102432","DOIUrl":"10.1016/j.aqrep.2024.102432","url":null,"abstract":"<div><div>This study evaluated the effects of dietary supplementation with different doses (0, 10, 20, 40, and 80 g kg⁻¹) of powdered avocado seed (AS) on the growth performance, immunological response, and gene expression of Nile tilapia (<em>Oreochromis niloticus</em>) reared in a biofloc system over 8 weeks. A total of 300 Nile tilapia fingerlings (average weight 14.67 ± 0.07 g) were randomly assigned to five treatment groups, each with three replicates, and 20 fish per tank. The results demonstrated significant improvements (<em>p &lt; 0.05</em>) in growth and immune response in AS-supplemented fish, particularly in those fed the 10 g kg⁻¹ AS diet (AS10), which showed the most notable increases. In contrast, fish fed higher AS doses (AS20, AS40, and AS80) exhibited no statistically significant differences compared to the control group (<em>p &gt; 0.05</em>). Additionally, the AS10 group exhibited a significant upregulation (<em>p &lt; 0.05</em>) in the mRNA expression of key immune-related genes (<em>IL-1</em>, <em>IL-8</em>, and <em>LBP</em>) and antioxidant-related genes (<em>GST-α, GPX</em>, and <em>GSR</em>) in both liver and intestinal tissues, indicating enhanced immune and antioxidant responses. The highest expression levels were found in the AS10 group. These findings suggest that the inclusion of 10 g kg⁻¹ powdered avocado seed in the diet substantially enhances growth, immune function, and gene expression in Nile tilapia reared in a biofloc system. The results highlight avocado seed as a promising feed additive for improving the sustainability of Nile tilapia aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102432"},"PeriodicalIF":3.2,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of different initial feed on growth performance, intestinal morphology and microbiota diversity, immunity response, and disease resistance of pacific short-finned glass eel, Anguilla bicolor pacifica","authors":"Yen-Ting Lin,&nbsp;Hsiang-Yi Hsu,&nbsp;Yu-San Han","doi":"10.1016/j.aqrep.2024.102436","DOIUrl":"10.1016/j.aqrep.2024.102436","url":null,"abstract":"<div><div>Currently, aquaculture fishermen predominantly use bloodworms (<em>Chironomus</em> spp. or <em>Tubifex</em> sp.) or eel powder as initial feed for the pacific short-finned glass eel, <em>Anguilla bicolor pacifica</em>. However, bloodworms thrive in waters rich in organic matter, making them prone to carrying pathogens into aquaculture systems, particularly <em>Edwardsiella tarda</em>, a severe bacterial pathogen for all <em>Anguilla</em> species. Therefore, this research explored the use of an artificial paste feed as the initial feed for pacific short-finned glass eels in the recirculating aquaculture system (RAS), evaluating growth performance, immune gene expression, disease resistance, and intestinal morphology and microbiota diversity across different initial feeds for glass eels. The paste feed demonstrated similar attractiveness and palatability to pacific short-finned glass eels as bloodworms, both of which were superior to eel powder. There were no significant differences in growth performance between the paste feed and bloodworm groups, but the survival rate in the paste feed group was significantly higher than in the bloodworm group. In terms of intestinal morphology, only the paste feed group showed no inflammation or abnormalities and had significantly longer villi. Moreover, the relative expression of superoxide dismutase (SOD) and peroxidase (POD) were significantly higher in the paste feed group compared with the bloodworm and powder groups, resulting in the best survival rate following an <em>E. tarda</em> challenge test. Additionally, the significantly lower expression of interleukin-6 (IL-6) and heat shock protein 70 (HSP70) indicates reduced stress and inflammation in the paste feed group. The biodiversity index indicated that the paste feed group showed a significantly lower alpha diversity than the bloodworm and powder groups, and harmful pathogens (such as <em>Aeromonas</em>) were not detected in the intestines. These results indicate that paste feed is safer and more efficient than bloodworms and eel powder for pacific short-finned glass eels.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102436"},"PeriodicalIF":3.2,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of light in the rearing of Clarias gariepinus larvae: Effects on behaviour, growth and survival","authors":"Marc-Christopher Hildebrand,&nbsp;Nina Hentrich,&nbsp;Harry Wilhelm Palm","doi":"10.1016/j.aqrep.2024.102420","DOIUrl":"10.1016/j.aqrep.2024.102420","url":null,"abstract":"<div><div>The growth performance and behavior activity of African catfish <em>Clarias gariepinus</em> (Burchell 1822) larvae were examined under different light regimes. Larvae (average weight 0.095 g / length 2.28 cm) were reared in 50 L aquaria at a stocking density of 12 fish/L and challenged with red, yellow or blue light. In a second experiment, larvae from the same batch were challenged with different yellow light intensities of 0.07, 23.00 or 70.00 (W/m²/nm). Performance parameters were determined by fish counts as well as measuring the initial and final body weight. Video recordings were applied in order to evaluate ethological parameters. African catfish larvae were affected by different light spectra as seen in the number of lesions and swimming activity-, escape- and air-breathing behavior, where the subgroups with red and yellow color differed from the blue treatment. The swimming- and air-breathing activity were positively affected under low light intensities. The number of lesions also decreased under minimal illumination. The performance indicators feeding conversion rate (FCR), special growth rate (SGR) or the final mean weight were not affected by any light treatment. Fish mortality and growth performance were not significantly influenced by the respective light treatment, we therefore recommend that yellow and dimmed light is most suitable for the initial rearing of African catfish larvae with respect to improved fish welfare.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102420"},"PeriodicalIF":3.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acute hypoxia and reoxygenation on the coelomic fluid of Phascolosoma esculenta: Oxidative stress and transcriptome analysis","authors":"Jiamin Xing ,&nbsp;Jie Zou ,&nbsp;Xiaodan Liu ,&nbsp;RiQuan Liao ,&nbsp;Muhammad Farhan Khan ,&nbsp;Mengqing Zeng ,&nbsp;Congyan Yu ,&nbsp;Wu Xiaoke ,&nbsp;Xueyu Yan ,&nbsp;Hong Zhang ,&nbsp;Lei Bao ,&nbsp;Huijing Peng ,&nbsp;Peng Zhu ,&nbsp;Laiba Shafique","doi":"10.1016/j.aqrep.2024.102424","DOIUrl":"10.1016/j.aqrep.2024.102424","url":null,"abstract":"<div><div><em>Phascolosoma esculenta</em> is an economically species inhabiting a soft substrate of mud in the intertidal zone, which is bad breathability, especially during the prolonged heavy rainstorms, that will bring hypoxia environment to <em>P. esculenta.</em> In this study, the negative impact of hypoxia on <em>P. esculenta</em> was firstly studied<em>.</em> The results showed that there were no <em>P. esculenta</em> died post 7 days hypoxia. However, <em>P. esculenta</em> surface became black after hypoxia stress and changed to brown after reoxygenation. The body cavity fluid’s total antioxidant capacity (T-AOC) showed notable increases at 48 h, 120 h, and 168 h post hypoxia stress and returned to the control level after reoxygenation. The contents of malondialdehyde (MDA) increased significantly at 24 h, 48 h, and 96 h but decreased substantially after reoxygenation. The activity of lactate dehydrogenase (LDH) increased during the hypoxia but decreased significantly after reoxygenation. Transcriptomic analysis was performed and a total of 55.26 GB of clean data were collected, 362 DEGs were obtained based on FPKM values, including 77 DEGs between control and hypoxia groups, 48 DEGs between control and reoxygenation groups, and 237 DEGs between hypoxia and reoxygenation groups. DEGs are enriched in pathways associated with immune system, carbon metabolism, apoptosis, ribosome and ion transport. These data enriched the molecular mechanism of invertebrate hypoxia tolerance and provided genetic material reference for future breeding of hypoxia-tolerant <em>P. esculenta</em> and other aquatic species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102424"},"PeriodicalIF":3.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylation profile of the testes of the flatfish Solea senegalensis","authors":"Daniel Ramírez ,&nbsp;María Esther Rodríguez ,&nbsp;Robert Mukiibi ,&nbsp;Carolina Peñaloza ,&nbsp;Helena D’Cotta ,&nbsp;Diego Robledo ,&nbsp;Laureana Rebordinos","doi":"10.1016/j.aqrep.2024.102405","DOIUrl":"10.1016/j.aqrep.2024.102405","url":null,"abstract":"<div><div>In aquaculture production, fish phenotypes are influenced by a combination of external and internal factors, while the underlying mechanisms often involve distinct DNA methylation patterns. These play a fundamental role in regulating gene expression in response to environmental shifts. The Senegalese sole (<em>Solea senegalensis</em>) holds significant potential in aquaculture due to its high commercial importance. However, its production faces several challenges, including the inability of F1 soles to reproduce. In our study, we analysed the methylation patterns in the testicular DNA of 12 male <em>S. senegalensis</em> individuals from four distinct groups, which differed in rearing origins (wild and F1 soles) and sexual maturity stages (mature and immature). We employed reduced representation bisulfite sequencing to identify significant methylation differences among studied groups. The differential methylation analysis showed a substantial disparity between the F1 and wild groups. This difference was evident in both the number of different methylated CpGs (DMCpGs) and methylation levels, highlighting a distinction in groups reared under different conditions. The comparison between immature and mature wild groups revealed notable differences, suggesting that the testes methylation profile undergoes few changes in wild individuals during sexual maturity. These differences in methylation between wild and hatchery-born and reared offspring imply epigenetic modifications triggered by rearing in captivity and by the absence of natural <em>stimuli</em> such as temperature fluctuations. We further annotated the DMCpGs based on their position and co-localization with genes in the Senegalese sole genome. Our analysis revealed that the methylation differences observed in DMCpGs were more pronounced in intron regions, with higher methylation levels. Conversely, the methylation differences in exon and promoter areas were more subtle. Upon annotation, we discovered that these DMCpG sites were located near or within various transcription factors. Our comprehensive analysis sheds light on the intricate methylation profile of <em>S. senegalensis</em>, focusing on genes associated with reproductive traits, particularly those related to sex determination (SD) systems and spermatogenesis. Overall, we observe a trend toward higher methylation levels in DMCpGs when comparing F1 individuals to wild groups, as well as immature to mature wild groups. Hence, sexual maturity and place of origin resulted in marked methylation differences, which could potentially affect the expressions of genes involved in both development and reproduction of farmed males. This analysis explores the gene-regulating effects of this epigenetic mechanism providing valuable insights into the variations in methylation found in the testes of Senegalese sole.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102405"},"PeriodicalIF":3.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)","authors":"Changyou Song ,&nbsp;Jianxiang Chen ,&nbsp;Jiyan He ,&nbsp;Miaomiao Xue ,&nbsp;Pao Xu ,&nbsp;Huiming Ding ,&nbsp;Ningyuan Wu ,&nbsp;Ruicheng Zhao ,&nbsp;Gangchun Xu ,&nbsp;Hongxia Li","doi":"10.1016/j.aqrep.2024.102428","DOIUrl":"10.1016/j.aqrep.2024.102428","url":null,"abstract":"<div><div>The nutritive value and maturity status of hepatopancreases dominates the quality of Chinese mitten crab, <em>Eriocheir sinensis</em>. However, the underlying function and regulatory mechanism, as well as the external nutrient factors on the development of hepatopancreases in different growth stages of E. sinensis remains unclear. In this study, we first revealed the function of hepatopancreases feeding with iced trash fish (ITF) during the whole culture period; and further investigated the underlying mechanism on nutrient deposition and flavor yield feeding with formulated feed (FEED) in replacement of ITF in female <em>E. sinensis</em>. From the results, growth performance and metabolism indicate <em>E. sinensis</em> was robustly developed, and ITF promotes growth and hepatopancreases development during the maturation period. Meanwhile, activation of fatty acid and amino acid metabolism in hepatopancreases resulted in depletion of monounsaturated fatty acids (MUFAs) but promoted protein deposition during the maturation stage. Integrated analysis of transcriptome and metabolome reveal metabolism, biosynthesis, and flavor presentation were intensively active during maturation, evidenced by the expression of key genes of <em>ALDH</em>, <em>TBXAs</em>, <em>HADHA</em>, <em>UPB1</em>, <em>CYP2</em>, <em>GST</em>, and <em>γ-GT-X1</em>; as well as the key metabolites of GMP and IMP. Additionally, substitution of ITF with FEED further confirm full price artificial feed could meet the nutrient requirements during the maturation period, evidenced by the growth performance, fatty acid and amino acid derived nutrient and flavor yield in hepatopancreases. In conclusion, nutrient and flavor deposition was associated with the fast development of hepatopancreases, and fatten with full price compound feed contributes to the nutrient requirement and flavor yield during the maturation stage.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102428"},"PeriodicalIF":3.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142437830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}