Pub Date : 2024-10-30DOI: 10.1016/j.aqrep.2024.102450
Duanduan Chen , Zhiwei Wang , Xiangkai Li , Hongchi Du , Kaisheng Zhang , Shengliang Cao , Jianbiao Lu , Shuang Zhao , Hui Wang , Yubao Li
Vibrio parahaemolyticus (V. parahaemolyticus) is an opportunistic pathogen primarily affecting aquaculture animals, leading to severe aquatic animal diseases and fatalities. Furthermore, the consumption of undercooked seafood contaminated with this strain can lead to acute gastroenteritis in humans. Bacteriophages are considered as potential antibiotic alternatives that could effectively mitigate the harmful impacts of Vibrio. The complex relationship between phages and their hosts is still not fully understood. By exploring the fundamental biological characteristics of phages and the transcription of host mRNA during interactions with their hosts, we can systematically investigate the regulatory networks that govern the function of host molecules produced by both phages and bacteria. We isolated a lytic V. parahaemolyticus phage, vB-VpaS-SD15 (P15), from a shrimp culture pond. Transmission electron microscopy revealed that P15 is a member of the Siphoviridae family of tailed phages. The host spectrum of 138 V. parahaemolyticus strains isolated from aquaculture ponds in the southeastern coast of China was evaluated, indicating that this phage strain could lyse 33 V. parahaemolyticus strains efficiently within a a brief culture period. Furthermore, it exhibited prolonged stability at temperatures up to 60°C, a broad pH range from 2 to 12, diverse forms, and an infection multiplier of 0.01, and the burst volume is about 23 PFU/mL. We completed an interaction map of the host bacterial and phage gene frameworks. Host bacteria were collected at different time points (5 min, 20 min, 40 min, 60 min, 100 min) representing incubation, burst, and plateau phases of phage infection, and were depicted as one-step growth curves. RNA-seq technology was utilized to monitor changes in host bacterial mRNA transcription throughout phage infection. The results suggest that parasitic infection leads to alterations in host genes responsible for various cellular processes like ribosome biogenesis, tRNA biogenesis, secretion, protein export via ABC transporters, TCA cycle, glycolysis/uronic acid production, oxidative phosphorylation, and aminoacyl-tRNA biosynthesis. Notably, genes involved in signaling pathways, such as ribosomal protein genes and multiple antibiotic resistance regulator (MarR) genes, showed significant changes. However, no notable changes were observed in genes associated with the host bacterial classical immune defense system, like the CRISPR system, during the observed time period. These findings offer valuable insights into the alterations in host genes during phage infection, shedding light on the regulatory mechanisms governing the interaction between phages and their hosts. This study lays the groundwork for the potential application of phages in aquaculture production.
{"title":"Biological properties of Vibrio parahaemolyticus lytic phages and transcriptome analysis of their interactions with the host","authors":"Duanduan Chen , Zhiwei Wang , Xiangkai Li , Hongchi Du , Kaisheng Zhang , Shengliang Cao , Jianbiao Lu , Shuang Zhao , Hui Wang , Yubao Li","doi":"10.1016/j.aqrep.2024.102450","DOIUrl":"10.1016/j.aqrep.2024.102450","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> (<em>V. parahaemolyticus</em>) is an opportunistic pathogen primarily affecting aquaculture animals, leading to severe aquatic animal diseases and fatalities. Furthermore, the consumption of undercooked seafood contaminated with this strain can lead to acute gastroenteritis in humans. Bacteriophages are considered as potential antibiotic alternatives that could effectively mitigate the harmful impacts of <em>Vibrio</em>. The complex relationship between phages and their hosts is still not fully understood. By exploring the fundamental biological characteristics of phages and the transcription of host mRNA during interactions with their hosts, we can systematically investigate the regulatory networks that govern the function of host molecules produced by both phages and bacteria. We isolated a lytic <em>V. parahaemolyticus</em> phage, <em>vB-VpaS-SD15</em> (P15), from a shrimp culture pond. Transmission electron microscopy revealed that P15 is a member of the Siphoviridae family of tailed phages. The host spectrum of 138 <em>V. parahaemolyticus</em> strains isolated from aquaculture ponds in the southeastern coast of China was evaluated, indicating that this phage strain could lyse 33 <em>V. parahaemolyticus</em> strains efficiently within a a brief culture period. Furthermore, it exhibited prolonged stability at temperatures up to 60°C, a broad pH range from 2 to 12, diverse forms, and an infection multiplier of 0.01, and the burst volume is about 23 PFU/mL. We completed an interaction map of the host bacterial and phage gene frameworks. Host bacteria were collected at different time points (5 min, 20 min, 40 min, 60 min, 100 min) representing incubation, burst, and plateau phases of phage infection, and were depicted as one-step growth curves. RNA-seq technology was utilized to monitor changes in host bacterial mRNA transcription throughout phage infection. The results suggest that parasitic infection leads to alterations in host genes responsible for various cellular processes like ribosome biogenesis, tRNA biogenesis, secretion, protein export via ABC transporters, TCA cycle, glycolysis/uronic acid production, oxidative phosphorylation, and aminoacyl-tRNA biosynthesis. Notably, genes involved in signaling pathways, such as ribosomal protein genes and multiple antibiotic resistance regulator (MarR) genes, showed significant changes. However, no notable changes were observed in genes associated with the host bacterial classical immune defense system, like the CRISPR system, during the observed time period. These findings offer valuable insights into the alterations in host genes during phage infection, shedding light on the regulatory mechanisms governing the interaction between phages and their hosts. This study lays the groundwork for the potential application of phages in aquaculture production.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102450"},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Precocial Eriocheir sinensis, showing advanced sexual maturity, are a serious impediment to crab industry. The aim of this study was to identify miRNAs and its related pathways involved in regulation of precocity in E. sinensis. High-throughput sequencing was used to evaluate hepatopancreas of precocious and normal E. sinensis at the same period. A total of 101 differentially expressed miRNAs were identified as candidate target genes. KEGG analysis revealed retinol metabolism may contribute to the regulation of gonadogenesis. Aldehyde dehydrogenase 1 family member A1 (aldh1a1) was identified as the core gene in the retinol metabolism by gene expression, and subsequently, gene cloning was performed to further explore its function. The aldh1a1 gene was 1740 bp, including a 189 bp 5′ untranslated region (UTR), 75 bp 3′ UTR and a 1476 bp open-reading frame, encoding 491 amino acids. The corresponding protein is 87.98 % homologous in Portunus trituberculatus. The expression of aldh1a1 was highest in ovary, and then in the hepatopancreas. In addition, dual-luciferase reporter assay indicated that there was negative correlation between NW_020868524.1_2213 and aldh1a1. NW_020868524.1_2213 agomir treatment in the normal group increased testosterone and estradiol levels significantly, down-regulated aldh1a1 expression, and activated the retinol metabolism. By contrast, the administration of NW_020868524.1_2213 antagomir after a short-term induction of retinol resulted in increase in aldh1a1 expression, decrease in sex hormone levels, and inhibition in retinol metabolism. In short, NW_020868524.1_2213 in the hepatopancreas could activate the retinol metabolic pathway via aldh1a1, thus regulating precocious and gonadal development in E. sinensis. These findings would provide a potential pathway for decreasing crab precocity in practice.
{"title":"Molecular mechanisms underlying sexual precocity in Chinese mitten crab (Eriocheir sinensis): aldh1a1 regulates retinol metabolism via miRNAs in the hepatopancreas","authors":"Xiaoyan Jia , Weibo Jiang , Ronghua Zhou , Wei Qiang , Xuejiao Lv , Le Chang , Qiyong Qiu , Wenbin Liu , Dingdong Zhang","doi":"10.1016/j.aqrep.2024.102460","DOIUrl":"10.1016/j.aqrep.2024.102460","url":null,"abstract":"<div><div>Precocial <em>Eriocheir sinensis</em>, showing advanced sexual maturity, are a serious impediment to crab industry. The aim of this study was to identify miRNAs and its related pathways involved in regulation of precocity in <em>E. sinensis</em>. High-throughput sequencing was used to evaluate hepatopancreas of precocious and normal <em>E. sinensis</em> at the same period. A total of 101 differentially expressed miRNAs were identified as candidate target genes. KEGG analysis revealed retinol metabolism may contribute to the regulation of gonadogenesis. Aldehyde dehydrogenase 1 family member A1 (<em>aldh1a1</em>) was identified as the core gene in the retinol metabolism by gene expression, and subsequently, gene cloning was performed to further explore its function. The a<em>ldh1a1</em> gene was 1740 bp, including a 189 bp 5′ untranslated region (UTR), 75 bp 3′ UTR and a 1476 bp open-reading frame, encoding 491 amino acids. The corresponding protein is 87.98 % homologous in <em>Portunus trituberculatus</em>. The expression of <em>aldh1a1</em> was highest in ovary, and then in the hepatopancreas. In addition, dual-luciferase reporter assay indicated that there was negative correlation between NW_020868524.1_2213 and <em>aldh1a1</em>. NW_020868524.1_2213 agomir treatment in the normal group increased testosterone and estradiol levels significantly, down-regulated <em>aldh1a1</em> expression, and activated the retinol metabolism. By contrast, the administration of NW_020868524.1_2213 antagomir after a short-term induction of retinol resulted in increase in <em>aldh1a1</em> expression, decrease in sex hormone levels, and inhibition in retinol metabolism. In short, NW_020868524.1_2213 in the hepatopancreas could activate the retinol metabolic pathway via <em>aldh1a1</em>, thus regulating precocious and gonadal development in <em>E. sinensis.</em> These findings would provide a potential pathway for decreasing crab precocity in practice.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102460"},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study evaluated the benefits of supplementing Nile tilapia diets with probiotics Saccharomyces boulardii (SB) and Bifidobacterium bifidum (BB) in terms of growth, antioxidant status, immune-related genes, and resistance against heat stress. Fish (with an initial weight of 3.85 ± 0.50 g) organized into four triplicate treatment groups defined as control (T0, no supplementation), Saccharomyces boulardii (T1, 1010 CFU/g), Bifidobacterium bifidum (T2, 1.5×10 8 CFU/ mL) and a mixture of both (T3). This trial continued over a 56-day feeding period and fish were sampled for analysis and thereafter, Nile tilapia was exposed to heat shock under a high temperature of 32°C for 48 hours. Results revealed that the highest final weight (FW), weight gain (WG), and specific growth rate (SGR) as well as significantly lower feed conversion ratio (FCR) were observed in the T3 compared to other groups (P < 0.05). Alternative complement activity (ACH50), total Protein (TP), and lysozyme (LZM) improved in fish-fed supplemented diets, and the highest value was observed in T2 and T3 (P < 0.05). Moreover, this combination enhanced antioxidant status, as evidenced by elevated levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT), reflecting improved defense against oxidative stress (P < 0.05). Gene expression analyses revealed increased expression of immune-related genes such as Interleukin 8, Interleukin10, Interleukin1β, tumor necrosis factor, and LYZ as well as antioxidant genes including CAT, Gpx, glutathione-disulfide reductase, SOD, in test groups compared to the control group (P < 0.05). The survival rate in Nile tilapia after heat stress was significantly higher in T3 compared to other groups (P < 0.05). It was concluded from this experiment that dietary inclusion of the probiotics species in combination could be promote growth and immune responses of O. niloticus.
{"title":"Enhancing growth performance, antioxidant defense, immunity response, and resistance against heat stress in Nile Tilapia (Oreochromis niloticus) fed Saccharomyces boulardii and/or Bifidobacterium bifidum","authors":"Nika Mashhadizadeh , Shiva Khezri , Delaram Eslimi Esfahani , Sedigheh Mohammadzadeh , Ehsan Ahmadifar , Mehdi Ahmadifar , Mohsen Shahriari Moghadam , Ehab El-Haroun","doi":"10.1016/j.aqrep.2024.102462","DOIUrl":"10.1016/j.aqrep.2024.102462","url":null,"abstract":"<div><div>This study evaluated the benefits of supplementing Nile tilapia diets with probiotics <em>Saccharomyces boulardii</em> (SB) and <em>Bifidobacterium bifidum</em> (BB) in terms of growth, antioxidant status, immune-related genes, and resistance against heat stress. Fish (with an initial weight of 3.85 ± 0.50 g) organized into four triplicate treatment groups defined as control (T0, no supplementation), <em>Saccharomyces boulardii</em> (T1, 10<sup>10</sup> CFU/g), <em>Bifidobacterium bifidum</em> (T2, 1.5×10 8 CFU/ mL) and a mixture of both (T3). This trial continued over a 56-day feeding period and fish were sampled for analysis and thereafter, Nile tilapia was exposed to heat shock under a high temperature of 32°C for 48 hours. Results revealed that the highest final weight (FW), weight gain (WG), and specific growth rate (SGR) as well as significantly lower feed conversion ratio (FCR) were observed in the T3 compared to other groups (P < 0.05). Alternative complement activity (ACH50), total Protein (TP), and lysozyme (LZM) improved in fish-fed supplemented diets, and the highest value was observed in T2 and T3 (P < 0.05). Moreover, this combination enhanced antioxidant status, as evidenced by elevated levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT), reflecting improved defense against oxidative stress (P < 0.05). Gene expression analyses revealed increased expression of immune-related genes such as Interleukin 8, Interleukin10, Interleukin1β, tumor necrosis factor, and LYZ as well as antioxidant genes including CAT, Gpx, glutathione-disulfide reductase, SOD, in test groups compared to the control group (P < 0.05). The survival rate in Nile tilapia after heat stress was significantly higher in T3 compared to other groups (P < 0.05). It was concluded from this experiment that dietary inclusion of the probiotics species in combination could be promote growth and immune responses of <em>O. niloticus</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102462"},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-30DOI: 10.1016/j.aqrep.2024.102454
Mahnoor Saleem , Syed Makhdoom Hussain , Shafaqat Ali , Pallab K. Sarker , Mudassar Shahid , Adan Naeem
Herbal supplements can effectively boost fish growth and are a cost-effective alternative to antibiotics. The main objective of this research was to explore the efficacy of leaf extract of Solanum nigrum (SNE) on the growth parameters, digestibility, carcass, blood indices, and antioxidant status of Oreochromis niloticus fingerlings (5.75±0.03 g/fish). Canola meal was used as basal diet. Six experimental treatments having different levels of SNE (0 %, 0.5 %, 1 %, 1.5 %, 2 % and 2.5 %) were formulated. 15 fingerlings were placed in each of 6 triplicate tanks. The feeding trial continued for 70 days and then the results showed improvement in growth indices, body composition (fat, protein, ash and moisture) and nutrient absorption (crude fat, gross energy and crude protein) of O. niloticus. Moreover, SNE treatment showed better results of blood indices. Best outcomes for all these parameters were observed at 1.5 % SNE supplementation (test diet-IV). Additionally, SNE treatment enhanced the antioxidant activity of O. niloticus. The serum antioxidant enzymes status (catalase, superoxide dismutase and peroxidase) were enhanced by SNE treatment. Antioxidant activity increased with increasing SNE supplementation. In conclusion, SNE improved the growth and overall health of O. niloticus and the optimum inclusion level suggested for SNE is 1.5 %. However, further research is necessary to ensure its safe and effective use in different fish species.
{"title":"New insights into the effect of Solanum nigrum extract supplementation on growth, digestibility, carcass, hematological indices, and antioxidant status in Oreochromis niloticus","authors":"Mahnoor Saleem , Syed Makhdoom Hussain , Shafaqat Ali , Pallab K. Sarker , Mudassar Shahid , Adan Naeem","doi":"10.1016/j.aqrep.2024.102454","DOIUrl":"10.1016/j.aqrep.2024.102454","url":null,"abstract":"<div><div>Herbal supplements can effectively boost fish growth and are a cost-effective alternative to antibiotics. The main objective of this research was to explore the efficacy of leaf extract of <em>Solanum nigrum</em> (SNE) on the growth parameters, digestibility, carcass, blood indices, and antioxidant status of <em>Oreochromis niloticus</em> fingerlings (5.75±0.03 g/fish). Canola meal was used as basal diet. Six experimental treatments having different levels of SNE (0 %, 0.5 %, 1 %, 1.5 %, 2 % and 2.5 %) were formulated. 15 fingerlings were placed in each of 6 triplicate tanks. The feeding trial continued for 70 days and then the results showed improvement in growth indices, body composition (fat, protein, ash and moisture) and nutrient absorption (crude fat, gross energy and crude protein) of <em>O. niloticus</em>. Moreover, SNE treatment showed better results of blood indices. Best outcomes for all these parameters were observed at 1.5 % SNE supplementation (test diet-IV). Additionally, SNE treatment enhanced the antioxidant activity of <em>O. niloticus</em>. The serum antioxidant enzymes status (catalase, superoxide dismutase and peroxidase) were enhanced by SNE treatment. Antioxidant activity increased with increasing SNE supplementation. In conclusion, SNE improved the growth and overall health of <em>O. niloticus</em> and the optimum inclusion level suggested for SNE is 1.5 %. However, further research is necessary to ensure its safe and effective use in different fish species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102454"},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One common environmental stressor in fisheries is hypoxia stress, which frequently causes significant fish mortality and financial losses. This study aimed to examine the potential utility of serum concentrations of hypoxia-inducible factor-1α (HIF-1α) as a biomarker in fat greenlings, Hexagrammos otakii, under hypoxic stress.
Methods
The phylogenetic analysis of HIF-1α was carried out. Then, fat greenlings (Hexagrammous otakii) were subjected to hypoxia (DO: 2.2 ± 0.2 mg/L) for 6 h, 12 h, 24 h, 48 h. Upon admission under hypoxia stress, serum HIF-1α concentrations were measured using an enzyme-linked immunosorbent assay (ELISA). The outcomes of the laboratory tests were collected. Then, correlation analysis and regression equation fitting were performed.
Results
In this study, HoHIF-1α was determined by bioinformatics analysis. The relative expression of hemoglobinβ2 (HBβ2), lactate dehydrogenase (LDH), endoplasmic reticulum oxidase-1α (ERO-1α), and HIF-1α and the level of HB, LA, ERO-1α and HIF-1α were increased significantly. The hypoxia stress group had considerably greater serum HIF-1α concentrations in comparison to the control group. Furthermore, compared to lactate concentrations, HIF-1α concentrations were linked to a superior predictive capacity for hypoxia stress diagnosis. Then, the correlation analysis result showed that HIF-1α was positively correlated with hemoglobin (HB), ERO-1α, and lactic acid (LA). Meanwhile, HIF-1α has a good linear relationship with HB, ERO-1α, and LA respectively. The HIF-1α concentrations increase of more than 30.27 pg/mL indicates severe hypoxia stress in the H. otakii.
Conclusions
HIF-1α was positively correlated with HB, ERO-1α, and LA in H. otakii under hypoxia stress. Serum HIF-1α concentrations are a valuable indicator for hypoxia stress diagnosis and H. otakii recovery status prediction.
{"title":"Hypoxia-inducible factor-1α as a biomarker for individuals under hypoxia duration and pattern in fat greenling Hexagrammos otakii","authors":"Yu Zhan , Xin Qi , Yiting Wu , Dongxu Gao , Ling Zhao , Shengnan Cao , Zhuang Xue , Wei Wang","doi":"10.1016/j.aqrep.2024.102459","DOIUrl":"10.1016/j.aqrep.2024.102459","url":null,"abstract":"<div><h3>Background</h3><div>One common environmental stressor in fisheries is hypoxia stress, which frequently causes significant fish mortality and financial losses. This study aimed to examine the potential utility of serum concentrations of hypoxia-inducible factor-1α (HIF-1α) as a biomarker in fat greenlings, <em>Hexagrammos otakii</em>, under hypoxic stress.</div></div><div><h3>Methods</h3><div>The phylogenetic analysis of <em>HIF-1α</em> was carried out. Then, fat greenlings (<em>Hexagrammous otakii</em>) were subjected to hypoxia (DO: 2.2 ± 0.2 mg/L) for 6 h, 12 h, 24 h, 48 h. Upon admission under hypoxia stress, serum HIF-1α concentrations were measured using an enzyme-linked immunosorbent assay (ELISA). The outcomes of the laboratory tests were collected. Then, correlation analysis and regression equation fitting were performed.</div></div><div><h3>Results</h3><div>In this study, <em>HoHIF-1α</em> was determined by bioinformatics analysis. The relative expression of hemoglobinβ2 (<em>HBβ2</em>), lactate dehydrogenase (<em>LDH</em>), endoplasmic reticulum oxidase-1α (<em>ERO-1α</em>), and <em>HIF-1α</em> and the level of HB, LA, ERO-1α and HIF-1α were increased significantly. The hypoxia stress group had considerably greater serum HIF-1α concentrations in comparison to the control group. Furthermore, compared to lactate concentrations, HIF-1α concentrations were linked to a superior predictive capacity for hypoxia stress diagnosis. Then, the correlation analysis result showed that HIF-1α was positively correlated with hemoglobin (HB), ERO-1α, and lactic acid (LA). Meanwhile, HIF-1α has a good linear relationship with HB, ERO-1α, and LA respectively. The HIF-1α concentrations increase of more than 30.27 pg/mL indicates severe hypoxia stress in the <em>H. otakii</em>.</div></div><div><h3>Conclusions</h3><div>HIF-1α was positively correlated with HB, ERO-1α, and LA in <em>H. otakii</em> under hypoxia stress<em>.</em> Serum HIF-1α concentrations are a valuable indicator for hypoxia stress diagnosis and <em>H. otakii</em> recovery status prediction.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102459"},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.aqrep.2024.102431
Yanxia Chen , Caixia He , Ying Yang , Linan Wang , Zhaonan Li , Qingchun Yan , Tianxiu Liang , Changhong Bao , Wenjie Jin , Changzhong Li
This study aimed to investigate the effects of nano-selenium (nano-se) as a dietary supplement on Tibetan naked carp (Gymnocypris przewalskii) and six separate groups were conducted (0 mg/kg, NSEC; 2 mg/kg, NSE2; 4 mg/kg, NSE4; 7 mg/kg, NSE7; 9 mg/kg, NSE9; and 11 mg/kg, NSE11). The growth performance, hemato-biochemical activity, antioxidant activity, immune indices, and intestinal microbiota were subsequently measured at the end of the feeding experiment. The results showed that the hepatosomatic index, viscerosomatic index and condition factor were significantly greater in the NSE4 group (P < 0.05). The NSE4 group presented the highest specific growth rate and weight gain rate, whereas the NSE11 group presented the lowest rates. The survival rate was greater in the NSE2, NSE4, and NSE9 groups, whereas it was lowest in the NSE11 group. The contents of TP and TG and the activities of T-AOC, GSH-Px, T-SOD, CAT and GR first increased but then decreased with increasing dietary selenium content. Compared with the other groups, the NSE4 group presented comparatively lower levels of proinflammatory factors such as IL-1β, IL-6, and TNF-α, but higher amounts of lysozyme LZM and immunoglobulin IgM. The NSE4 and NSE2 groups presented considerably increased levels of anti-inflammatory factors (IL-10 and TGF-β) (P < 0.05). Dietary supplementation with an appropriate amount of nano-se can improve liver vacuolationand hepatocyte swelling in G. przewalskii, but excessive nano-se could cause liver damage. In addition, the results of intestinal microbiota detection revealed that dietary nano-se could improve the diversity of the intestinal microbiota and increase the abundance of beneficial bacteria in G. przewalskii. This research will enhance our understanding of the possible uses of nano-se and lay the groundwork for increasing the resource efficiency of G. przewalskii breeding and releasing.
{"title":"Effects of dietary nano-selenium on the growth performance, antioxidant capacity, immunity and intestinal microbiota of Tibetan naked carp (Gymnocypris przewalskii)","authors":"Yanxia Chen , Caixia He , Ying Yang , Linan Wang , Zhaonan Li , Qingchun Yan , Tianxiu Liang , Changhong Bao , Wenjie Jin , Changzhong Li","doi":"10.1016/j.aqrep.2024.102431","DOIUrl":"10.1016/j.aqrep.2024.102431","url":null,"abstract":"<div><div>This study aimed to investigate the effects of nano-selenium (nano-se) as a dietary supplement on Tibetan naked carp (<em>Gymnocypris przewalskii</em>) and six separate groups were conducted (0 mg/kg, NSEC; 2 mg/kg, NSE2; 4 mg/kg, NSE4; 7 mg/kg, NSE7; 9 mg/kg, NSE9; and 11 mg/kg, NSE11). The growth performance, hemato-biochemical activity, antioxidant activity, immune indices, and intestinal microbiota were subsequently measured at the end of the feeding experiment. The results showed that the hepatosomatic index, viscerosomatic index and condition factor were significantly greater in the NSE4 group (<em>P</em> < 0.05). The NSE4 group presented the highest specific growth rate and weight gain rate, whereas the NSE11 group presented the lowest rates. The survival rate was greater in the NSE2, NSE4, and NSE9 groups, whereas it was lowest in the NSE11 group. The contents of TP and TG and the activities of T-AOC, GSH-Px, T-SOD, CAT and GR first increased but then decreased with increasing dietary selenium content. Compared with the other groups, the NSE4 group presented comparatively lower levels of proinflammatory factors such as IL-1β, IL-6, and TNF-α, but higher amounts of lysozyme LZM and immunoglobulin IgM. The NSE4 and NSE2 groups presented considerably increased levels of anti-inflammatory factors (IL-10 and TGF-β) (<em>P</em> < 0.05). Dietary supplementation with an appropriate amount of nano-se can improve liver vacuolationand hepatocyte swelling in <em>G. przewalskii</em>, but excessive nano-se could cause liver damage. In addition, the results of intestinal microbiota detection revealed that dietary nano-se could improve the diversity of the intestinal microbiota and increase the abundance of beneficial bacteria in <em>G. przewalskii</em>. This research will enhance our understanding of the possible uses of nano-se and lay the groundwork for increasing the resource efficiency of <em>G. przewalskii</em> breeding and releasing.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102431"},"PeriodicalIF":3.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.aqrep.2024.102441
Yongan Li , Chuanqi Yu , Shuyao Li , Youjie Li , Zhiwen Yuan , Jie Pan , Qi Chen , Yaping Li , Qiubai Zhou , Zirui Wang
Our objective was to systematically evaluate the impact of substituting fish meal (FM) with poultry by-product meal (PBM) in fish feed on the growth and hepatic health of loach. Six diets with different levels of PBM (0 %-PBM0, 10 %-PBM10, 30 %-PBM30, 50 %-PBM50, 70 %-PBM70 and 90 %-PBM90 were formulated. After 8 weeks feeding trial, the highest weight gain (WG) was observed in 55 % PBM replacement ratio. Meanwhile, the lowest feed conversion ratio (FCR) were observed in 41.56 % PBM replacement ratio. In addition, dietary PBM induced hepatic oxidative stress including increasing the activity of aminotransferase from PBM0 to PBM50 group, whereas the activity of those enzymes was decreased from PBM50 to PBM90 group. Meanwhile, histological analysis showed that the lipid vacuoles in the liver were increased from PBM0 to PBM50 group, then decreased from PBM50 to PBM90 group (P< 0.05). Moreover, primary bile acid biosynthesis was the only one pathway enriched in top 20 significant pathways by transcriptomic and metabolomic analysis. Specifically, the key bile acid synthesis gene CYP7a1 was down-regulated, while CYP7b1 evinced an up-regulated pattern in PBM50 group compared to PBM0 group. In short, the PBM replacement ratio of up to 50 % in diets was appropriate for rearing loach, while higher PBM replacement ratio resulted in extremely oxidative stress in the liver.
{"title":"Effect of dietary replacement of fish meal by poultry by-product meal on the growth and hepatic health in loach (Paramisgurnus dabryanus)","authors":"Yongan Li , Chuanqi Yu , Shuyao Li , Youjie Li , Zhiwen Yuan , Jie Pan , Qi Chen , Yaping Li , Qiubai Zhou , Zirui Wang","doi":"10.1016/j.aqrep.2024.102441","DOIUrl":"10.1016/j.aqrep.2024.102441","url":null,"abstract":"<div><div>Our objective was to systematically evaluate the impact of substituting fish meal (FM) with poultry by-product meal (PBM) in fish feed on the growth and hepatic health of loach. Six diets with different levels of PBM (0 %-PBM0, 10 %-PBM10, 30 %-PBM30, 50 %-PBM50, 70 %-PBM70 and 90 %-PBM90 were formulated. After 8 weeks feeding trial, the highest weight gain (WG) was observed in 55 % PBM replacement ratio. Meanwhile, the lowest feed conversion ratio (FCR) were observed in 41.56 % PBM replacement ratio. In addition, dietary PBM induced hepatic oxidative stress including increasing the activity of aminotransferase from PBM0 to PBM50 group, whereas the activity of those enzymes was decreased from PBM50 to PBM90 group. Meanwhile, histological analysis showed that the lipid vacuoles in the liver were increased from PBM0 to PBM50 group, then decreased from PBM50 to PBM90 group (<em>P</em>< 0.05). Moreover, primary bile acid biosynthesis was the only one pathway enriched in top 20 significant pathways by transcriptomic and metabolomic analysis. Specifically, the key bile acid synthesis gene CYP7a1 was down-regulated, while CYP7b1 evinced an up-regulated pattern in PBM50 group compared to PBM0 group. In short, the PBM replacement ratio of up to 50 % in diets was appropriate for rearing loach, while higher PBM replacement ratio resulted in extremely oxidative stress in the liver.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102441"},"PeriodicalIF":3.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-26DOI: 10.1016/j.aqrep.2024.102452
Bi-Yun Zhang , Hong-Ling Yang , Ze-Hui Yuan , Xiao-Ou Sun , Jia-Hao Yin , Jun-Ji Xu , Guo-He Cai , Yun-Zhang Sun
<div><div>Largemouth bass (<em>Micropterus salmoides</em>) is poor at utilizing starch, and it is not clear whether commensal microorganisms can alleviate the adverse influences of high wheat starch in <em>M. salmoides.</em> Here, twelve autochthonous microorganisms with amylase producing abilities were isolated from the <em>M. salmoides</em> fed with diets containing 6 %, 12 %, or 18 % starch. Among them, <em>Citrobacter freundii</em> F1, <em>Bacillus altitudinis</em> F7, <em>Enterococcus casseliflavus</em> D12, <em>Plesiomonas shigelloides</em> D15, <em>Bacillus cabrialesii</em> D16, <em>Citrobacter portucalensis</em> K25 and <em>Serratia oryzae</em> K28 showed no hemolytic activity and were safety for <em>M. salmoides</em>. A 42-d feeding experiment was performed to study the functions of the seven microorganisms. A total of 810 fish (5.9 ± 0.01 g) were equally distributed into 9 groups. The control groups C6 and C18 were respectively fed with 6 % and 18 % wheat starch diets, the groups F1, F7, D12, D15, D16, K25 and K28 were fed with 18 % wheat starch diets respectively supplemented with 1.0×10<sup>8</sup> CFU/g <em>C. freundii</em> F1, <em>B. altitudinis</em> F7, <em>E. casseliflavus</em> D12, <em>P. shigelloides</em> D15, <em>B. cabrialesii</em> D16, <em>C. portucalensis</em> K25 and <em>S. oryzae</em> K28. The results indicated that compared with 6 % starch, 18 % level inhibited the growth performance, increased the hepatic glycogen and fat contents, improved the activities of glycolysis-related enzymes (hexokinase (HK), glucokinase (GK), phosphofructokinase (PFK), pyruvate kinase (PK)), decreased the activities of gluconeogenesis-related enzymes, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK), promoted the activity of lipid synthases-related enzyme carnitine palmitoyl transferaseⅠ (CPT1) and reduced the activity of lipolytic enzymes-related lipoprotein lipase (LPL). <em>C. freundii</em> F1, <em>P. shigelloides</em> D15, <em>C. portucalensis</em> K25 and <em>S. oryzae</em> K28 had relatively poor alleviating effects, while <em>B. altitudinis</em> F7, <em>E. casseliflavus</em> D12 and <em>B. cabrialesii</em> D16 significantly promoted the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER), decreased feed conversion rate (FCR), significantly reduced the hepatic glycogen and fat contents, decreased the activities of glycolysis-related enzymes (HK, GK, PK), improved the activities of gluconeogenesis-related enzymes (PC, PEPCK), reduced the activity of lipid synthases-related enzyme CPT1 (<em>P</em> < 0.05), which proved that these three strains could effectively alleviate the negative effects of high starch in <em>M. salmoides</em>. In conclusion, among the seven autochthonous microorganisms, <em>B. altitudinis</em> F7, <em>E. casseliflavus</em> D12 and <em>B. cabrialesii</em> D16 presented better potential advantages in improving glucose and lipid metabolisms
{"title":"Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides","authors":"Bi-Yun Zhang , Hong-Ling Yang , Ze-Hui Yuan , Xiao-Ou Sun , Jia-Hao Yin , Jun-Ji Xu , Guo-He Cai , Yun-Zhang Sun","doi":"10.1016/j.aqrep.2024.102452","DOIUrl":"10.1016/j.aqrep.2024.102452","url":null,"abstract":"<div><div>Largemouth bass (<em>Micropterus salmoides</em>) is poor at utilizing starch, and it is not clear whether commensal microorganisms can alleviate the adverse influences of high wheat starch in <em>M. salmoides.</em> Here, twelve autochthonous microorganisms with amylase producing abilities were isolated from the <em>M. salmoides</em> fed with diets containing 6 %, 12 %, or 18 % starch. Among them, <em>Citrobacter freundii</em> F1, <em>Bacillus altitudinis</em> F7, <em>Enterococcus casseliflavus</em> D12, <em>Plesiomonas shigelloides</em> D15, <em>Bacillus cabrialesii</em> D16, <em>Citrobacter portucalensis</em> K25 and <em>Serratia oryzae</em> K28 showed no hemolytic activity and were safety for <em>M. salmoides</em>. A 42-d feeding experiment was performed to study the functions of the seven microorganisms. A total of 810 fish (5.9 ± 0.01 g) were equally distributed into 9 groups. The control groups C6 and C18 were respectively fed with 6 % and 18 % wheat starch diets, the groups F1, F7, D12, D15, D16, K25 and K28 were fed with 18 % wheat starch diets respectively supplemented with 1.0×10<sup>8</sup> CFU/g <em>C. freundii</em> F1, <em>B. altitudinis</em> F7, <em>E. casseliflavus</em> D12, <em>P. shigelloides</em> D15, <em>B. cabrialesii</em> D16, <em>C. portucalensis</em> K25 and <em>S. oryzae</em> K28. The results indicated that compared with 6 % starch, 18 % level inhibited the growth performance, increased the hepatic glycogen and fat contents, improved the activities of glycolysis-related enzymes (hexokinase (HK), glucokinase (GK), phosphofructokinase (PFK), pyruvate kinase (PK)), decreased the activities of gluconeogenesis-related enzymes, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK), promoted the activity of lipid synthases-related enzyme carnitine palmitoyl transferaseⅠ (CPT1) and reduced the activity of lipolytic enzymes-related lipoprotein lipase (LPL). <em>C. freundii</em> F1, <em>P. shigelloides</em> D15, <em>C. portucalensis</em> K25 and <em>S. oryzae</em> K28 had relatively poor alleviating effects, while <em>B. altitudinis</em> F7, <em>E. casseliflavus</em> D12 and <em>B. cabrialesii</em> D16 significantly promoted the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER), decreased feed conversion rate (FCR), significantly reduced the hepatic glycogen and fat contents, decreased the activities of glycolysis-related enzymes (HK, GK, PK), improved the activities of gluconeogenesis-related enzymes (PC, PEPCK), reduced the activity of lipid synthases-related enzyme CPT1 (<em>P</em> < 0.05), which proved that these three strains could effectively alleviate the negative effects of high starch in <em>M. salmoides</em>. In conclusion, among the seven autochthonous microorganisms, <em>B. altitudinis</em> F7, <em>E. casseliflavus</em> D12 and <em>B. cabrialesii</em> D16 presented better potential advantages in improving glucose and lipid metabolisms","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102452"},"PeriodicalIF":3.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-26DOI: 10.1016/j.aqrep.2024.102433
Jeremiah Olanipekun Jimoh , Sharifah Rahmah , Bamidele Oluwarotimi Omitoyin , Emmanuel Kolawole Ajani , Mohamad Jalilah , Victor Tosin Okomoda , Donald Torsabo , Abiola Ayodeji Fabusoro , Yu Mei Chang , Young-Mao Chen , Hon Jung Liew
Jatropha curcas is an oil producing seed with high nutritional qualities for consumption. However, due to inherent anti-nutritional factors, appropriate processing is necessary to improve its nutrients utilization. This study was aimed at processing J. curcas using solvent-extraction method and evaluating its inclusion effects in the diet for Clarias gariepinus on its growth status. J. curcas meal was extracted using 80 % methanol to remove phorbol esters and other anti-nutrients. Thereafter, five iso-nitrogenous diets (40 % crude protein) were formulated containing J. curcas meal replacements of soybeans meal at 0 % (TRT0), 25 % (TRT25), 50 % (TRT50), 75 % (TRT75) and 100 % (TRT100) and fed to C. gariepinus for 70 days. Results showed that TRT25 fish had the highest mean weight gain and specific growth. The serum alkaline phosphate, alanine aminotransferase, and aspartate aminotransferase levels began to rise significantly (p ≤ 0.05) at 50 % replacement. Likewise, the assessment of gut ecology and morphology indicated that TRT25 had significantly (p ≤ 0.05) the highest gut bacteria colony forming unit (2.60 ×104±0.02cfu/g) and most favorable area of absorption (0.16±0.01 cm2). The histopathological observation of the fish intestine, liver and gills indicated no visible deformity in TRT0 and TRT25 fish. However, various degrees of degenerations were observed in the fish fed with 50 % and higher inclusion of J. curcas. This study showed that methanol-extracted J. curcas meal can be effectively utilized for C. gariepinus at 25 % of replacement.
{"title":"Growth performances of Clarias gariepinus juveniles fed with Jatropha curcas seed meal","authors":"Jeremiah Olanipekun Jimoh , Sharifah Rahmah , Bamidele Oluwarotimi Omitoyin , Emmanuel Kolawole Ajani , Mohamad Jalilah , Victor Tosin Okomoda , Donald Torsabo , Abiola Ayodeji Fabusoro , Yu Mei Chang , Young-Mao Chen , Hon Jung Liew","doi":"10.1016/j.aqrep.2024.102433","DOIUrl":"10.1016/j.aqrep.2024.102433","url":null,"abstract":"<div><div><em>Jatropha curcas</em> is an oil producing seed with high nutritional qualities for consumption. However, due to inherent anti-nutritional factors, appropriate processing is necessary to improve its nutrients utilization. This study was aimed at processing <em>J. curcas</em> using solvent-extraction method and evaluating its inclusion effects in the diet for <em>Clarias gariepinus</em> on its growth status<em>. J. curcas</em> meal was extracted using 80 % methanol to remove phorbol esters and other anti-nutrients. Thereafter, five iso-nitrogenous diets (40 % crude protein) were formulated containing <em>J. curcas</em> meal replacements of soybeans meal at 0 % (TRT0), 25 % (TRT25), 50 % (TRT50), 75 % (TRT75) and 100 % (TRT100) and fed to <em>C. gariepinus</em> for 70 days. Results showed that TRT25 fish had the highest mean weight gain and specific growth. The serum alkaline phosphate, alanine aminotransferase, and aspartate aminotransferase levels began to rise significantly (p ≤ 0.05) at 50 % replacement. Likewise, the assessment of gut ecology and morphology indicated that TRT25 had significantly (p ≤ 0.05) the highest gut bacteria colony forming unit (2.60 ×10<sup>4</sup>±0.02cfu/g) and most favorable area of absorption (0.16±0.01 cm<sup>2</sup>). The histopathological observation of the fish intestine, liver and gills indicated no visible deformity in TRT0 and TRT25 fish. However, various degrees of degenerations were observed in the fish fed with 50 % and higher inclusion of <em>J. curcas</em>. This study showed that methanol-extracted <em>J. curcas</em> meal can be effectively utilized for <em>C. gariepinus</em> at 25 % of replacement.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102433"},"PeriodicalIF":3.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-26DOI: 10.1016/j.aqrep.2024.102456
Léo GIRARD , Thomas A. DAVIDSON , Vincent TOLON , Tuba BUCAK , Soraya ROUIFED , Alexander WEZEL , Joël ROBIN
In the global carbon cycle, ponds can play a dual role: they have the capacity to store carbon through sedimentation, but also to be important greenhouse gas emitters. Of all the pond landscapes in Europe, many are managed by Humans for fish production and little is known today about their role as carbon sinks or sources. We monitored 20 fish ponds from the Dombes region (France) during a production season in 2022. We measured both sedimentation rates, the diffusion of CO2, CH4 and N2O and ebullition of CH4 over three different seasons (spring, summer and autumn) in order to provide an estimate of the carbon balance for each pond. Five were dried-out in 2023 as part of the management cycle, and were monitored for dry flux emissions during this period. On average, our measurements suggest that fish ponds were carbon sinks (6 fish ponds as sources and 14 as sinks). There was on average a net sequestration of 4.16 (+/- 10.00) tonnes of CO2e per hectare over 6 months. Measurements from the dry year, indicate that the ponds were carbon sources (mean value of 23.89 tonnes of CO2eq emitted). However, whilst the drying out phase directly increases emissions, it also encourages the development of macrophytes in the subsequent years which improves carbon storage through sedimentation, and appears to be an important driver of the observed balances. These data are consistent with the fact that fish ponds have the capacity to be either sources or sinks of carbon for the atmosphere. Moreover, by promoting good levels of primary productivity, the presence of aquatic plants and by improving carbon storage, management practices appear to have the capacity to turn fish ponds into carbon sinks. These agroecosystems could thus play an important role in the context of climate change mitigation.
{"title":"The balance of carbon emissions versus burial in fish ponds: The role of primary producers and management practices","authors":"Léo GIRARD , Thomas A. DAVIDSON , Vincent TOLON , Tuba BUCAK , Soraya ROUIFED , Alexander WEZEL , Joël ROBIN","doi":"10.1016/j.aqrep.2024.102456","DOIUrl":"10.1016/j.aqrep.2024.102456","url":null,"abstract":"<div><div>In the global carbon cycle, ponds can play a dual role: they have the capacity to store carbon through sedimentation, but also to be important greenhouse gas emitters. Of all the pond landscapes in Europe, many are managed by Humans for fish production and little is known today about their role as carbon sinks or sources. We monitored 20 fish ponds from the Dombes region (France) during a production season in 2022. We measured both sedimentation rates, the diffusion of CO<sub>2</sub>, CH<sub>4</sub> and N<sub>2</sub>O and ebullition of CH<sub>4</sub> over three different seasons (spring, summer and autumn) in order to provide an estimate of the carbon balance for each pond. Five were dried-out in 2023 as part of the management cycle, and were monitored for dry flux emissions during this period. On average, our measurements suggest that fish ponds were carbon sinks (6 fish ponds as sources and 14 as sinks). There was on average a net sequestration of 4.16 (+/- 10.00) tonnes of CO<sub>2</sub>e per hectare over 6 months. Measurements from the dry year, indicate that the ponds were carbon sources (mean value of 23.89 tonnes of CO<sub>2</sub>eq emitted). However, whilst the drying out phase directly increases emissions, it also encourages the development of macrophytes in the subsequent years which improves carbon storage through sedimentation, and appears to be an important driver of the observed balances. These data are consistent with the fact that fish ponds have the capacity to be either sources or sinks of carbon for the atmosphere. Moreover, by promoting good levels of primary productivity, the presence of aquatic plants and by improving carbon storage, management practices appear to have the capacity to turn fish ponds into carbon sinks. These agroecosystems could thus play an important role in the context of climate change mitigation.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102456"},"PeriodicalIF":3.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号