Aquaculture Reports最新文献

{"title":"How can competitiveness be maintained in deep-sea environments? Typical business model innovation and competitive strategies of marine aquaculture enterprises in China","authors":"Dahai Li ,&nbsp;Jingzhu Shan ,&nbsp;Wenhui Sun ,&nbsp;Limin Han","doi":"10.1016/j.aqrep.2026.103455","DOIUrl":"10.1016/j.aqrep.2026.103455","url":null,"abstract":"<div><div>Offshore aquaculture has advanced rapidly in recent years, with China leading in both the number of projects and capacity. Field investigations show that most offshore aquaculture enterprises in China adopt innovative operating models that differ from those nearshore aquaculture enterprises. This study takes cage farming enterprises as case studies and combines qualitative and quantitative data to analyze the characteristics of offshore aquaculture in terms of natural conditions and production costs. Four innovative operating models are identified: import substitution, wildization, temporary cultivation, and fishery-tourism integration. These models arise from the distinctive natural conditions of deep-sea areas, where high production costs and resource advantages prompt enterprises to adopt innovative strategies and differentiate themselves from nearshore competitors. Enabled by cage farming technology, the case enterprises identify potential market opportunities embedded in of deep-sea conditions, including arbitrage and innovation opportunities, and apply different resource innovation approaches, exploration and exploitation. These efforts yield four business model innovation strategies: element substitution, product innovation, capacity expansion, and format innovation. Across China’s offshore production settings, these strategies correspond to the four operating models observed in the cases. This study clarifies the economic characteristics and development patterns of offshore aquaculture and provides implications for firm strategy and aquaculture policy design.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103455"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of SNPs associated with body weight and muscle unsaturated fatty acids in grass carp (Ctenopharyngodon idella) through GWAS analysis","authors":"Zhi-fang Zhang ,&nbsp;Yuan-feng Xu ,&nbsp;Hao-ran Yang ,&nbsp;Xiu-lan Shi ,&nbsp;Jian-jun Fu ,&nbsp;Wen-bin Zhu ,&nbsp;Ming-kun Luo ,&nbsp;Zai-jie Dong ,&nbsp;Lan-mei Wang","doi":"10.1016/j.aqrep.2026.103434","DOIUrl":"10.1016/j.aqrep.2026.103434","url":null,"abstract":"<div><div>Grass carp (<em>Ctenopharyngodon idella</em>) has an important economic value in the world. In order to meet increasing high quality nutritional demands, there is an urgent need for genetic selection in breeding programs to improve fish muscle quality. Body weight (BW) and muscle unsaturated fatty acid (UFA) content are economically important traits in fish. In this study, we conducted a genome-wide association study (GWAS) based on whole-genome resequencing to identify single nucleotide polymorphisms (SNPs) and candidate genes potentially associated with BW and muscle UFA content in grass carp. A total of 4315,253 high-quality SNPs were identified and mapped onto 24 chromosomes. We identified 9, 4, 7 and 6 SNPs significantly associated with BW, UFA, polyunsaturated fatty acids (PUFA), and docosahexaenoic acid (DHA) content of fish muscle, respectively. Within the 100 kb upstream and downstream range of these significant SNPs, 51 candidate genes were associated with BW, while 48, 88 and 113 candidate genes were linked to muscle UFA, PUFA, and DHA content, respectively. Notably, <em>nfatc</em>2<em>a</em>, <em>mylk</em>4<em>a</em>, <em>ext</em>2 associated growth, <em>st</em>6<em>galnac</em>5, <em>hmgcra</em>, <em>Rab</em>8<em>a</em> and <em>ptges</em>3<em>a</em> associated with lipid metabolism. The findings of this study will facilitate the breeding of higher muscle UFA content new varieties of grass carp in the future.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103434"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary supplementation with distiller grain fermented liquid on growth performance, digestive and antioxidant capacity, and intestinal microbiota in juvenile Chinese mitten crab (Eriocheir sinensis)","authors":"Xiaogeng Chen ,&nbsp;Xiang Lu ,&nbsp;Fanfan Wu ,&nbsp;Yongchun Ge ,&nbsp;Changbo Wang ,&nbsp;Yunfei Sun ,&nbsp;Yongxu Cheng","doi":"10.1016/j.aqrep.2026.103441","DOIUrl":"10.1016/j.aqrep.2026.103441","url":null,"abstract":"<div><div>Distiller grain fermented liquid (DGFL) is a novel feed additive for aquaculture. In this study, we investigated the effects of DGFL supplementation on the growth and physiology of juvenile Chinese mitten crabs <em>Eriocheir sinensis</em>. Crabs were randomly divided into two groups and fed either a basal diet (C group) or a diet supplemented with 5 % DGFL (T group) for 140d. Their growth performance, digestive and antioxidant capacities, and intestinal microbiota were measured. The final average weight, weight gain rate, specific growth rate, and hepatosomatic index of the T group were higher than those of the C group (<em>P</em> &lt; 0.05). Crude protein and total amino acid contents in the T group increased (<em>P</em> &lt; 0.05). The expression levels of digestive enzyme-related genes (<em>CTRB1</em>, <em>TRYP</em>, <em>IL</em>, and <em>AMY</em>) and activities of α-amylase, lipase, trypsin, and pepsin were elevated in the T group (<em>P</em> &lt; 0.05). Total antioxidant capacity and superoxide dismutase and catalase activities in the hepatopancreas were enhanced in the T group (<em>P</em> &lt; 0.05), whereas malondialdehyde content decreased (<em>P</em> &lt; 0.05). The expression of antioxidant-related genes (<em>SOD</em>, <em>CAT</em>, <em>GPx</em>, and <em>Keap1</em>) increased (<em>P</em> &lt; 0.05). Furthermore, 5 % DGFL improved hepatopancreatic and intestinal structural integrity, increased the α-diversity of the intestinal microbiota (<em>P</em> &lt; 0.05), elevated the abundance of <em>Lactobacillales</em> and <em>Lactovum</em> (<em>P</em> &lt; 0.05), and reduced the abundance of the conditional pathogen <em>Vibrio</em>. In conclusion, dietary 5 % DGFL enhanced growth performance, digestive and antioxidant capacities, intestinal structure, and microbiota composition of juvenile <em>E. sinensis</em>. This study, the first to investigate DGFL supplementation in this species, provides a strong theoretical basis for its use in aquafeeds.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103441"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas anguilliseptica, an emerging pathogen for cultured largemouth bass (Micropterus salmoides) in China","authors":"Yilin Wang ,&nbsp;Kun Peng ,&nbsp;Keyu Zhou ,&nbsp;Mingqi Ai ,&nbsp;Qibin Jiang ,&nbsp;Jiao Wang ,&nbsp;Ping Ouyang ,&nbsp;Defang Chen ,&nbsp;Xiaoli Huang ,&nbsp;Yi Geng","doi":"10.1016/j.aqrep.2026.103406","DOIUrl":"10.1016/j.aqrep.2026.103406","url":null,"abstract":"<div><div><em>Pseudomonas anguilliseptica</em>, the causative agent of winter disease in marine fish, has inflicted severe economic losses on the aquaculture industry. In recent years, farmed largemouth bass (<em>Micropterus salmoides</em>) in China have suffered outbreaks of a novel disease marked by exophthalmia, dermal hemorrhage and ulceration, and multiple organ injuries, yet its etiology remains undetermined. In this study, three Gram-negative bacterial strains (SICAU PA001, SICAU PA002, and SICAU PA003) were isolated from diseased largemouth bass collected in different regions of Sichuan Province, China. To identify the pathogen and characterize its pathogenicity, morphological observation, antibiotic susceptibility tests, experimental challenge, <em>16S</em> rRNA gene analysis, multi-locus sequence analysis (MLSA), and complete genome sequencing analyses were conducted. The findings verified that the isolates were <em>P. anguilliseptica</em> and were classified as a novel sequence type (ST), ST8. Antibiotic susceptibility tests revealed that they were sensitive to florfenicol, levofloxacin, enrofloxacin, sulfisoxazole, co-trimoxazole, amoxicillin, and cefotaxime, but were remarkably resistant to aminoglycoside and lincoamide antibiotics. Experimental challenge demonstrated that the LD₅₀ to largemouth bass was 1.68 × 10⁵ CFU/kg. SICAU PA001 had a complete genome sequence of 5.24 Mbp and contained 5249 complete coding regions. By annotating the sequencing genes using the virulence factor database (VFDB) and the comprehensive antibiotic resistance database (CARD), it was determined that the genome contained 237 virulence genes and two antibiotic resistance genes. This study reports the first confirmed case of <em>P. anguilliseptica</em> isolation and identification from diseased largemouth bass in China, highlighting its potential role as a previously unrecognized pathogen in other freshwater fish species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103406"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrolyzed earthworm protein as a fishmeal substitute in rainbow trout diet: Effects on growth, nutrient utilization, digestive physiology, intestinal morphology, and growth-related gene expression","authors":"Negar Mohammadi,&nbsp;Houman Rajabi Islami ,&nbsp;Mehdi Shamsaie Mehrgan","doi":"10.1016/j.aqrep.2026.103386","DOIUrl":"10.1016/j.aqrep.2026.103386","url":null,"abstract":"<div><div>An eight-week feeding trial examined the effects of replacing fishmeal with earthworm protein hydrolysate (EPH) from <em>Eisenia fetida</em> on rainbow trout (<em>Oncorhynchus mykiss</em>). The fishmeal in a basal diet (43.29 % crude protein and 9.48 % crude lipid) was replaced with earthworm protein hydrolysate (EPH) at four levels of 5 % (EPH₅), 10 % (EPH₁₀), 15 % (EPH₁₅), 25 % (EPH₂₅), and earthworm protein at a level of 25 % (EP₂₅). Each experimental diet was allocated to a triplicate group of fish (initial body weight of 5.00 ± 0.10 g) in a flow-through system for eight weeks. At the end of the feeding trial, no significant differences in final body weight, weight gain ratio, average daily gain, specific growth rate, or feed conversion ratio were detected between the fish fed diets supplemented with different levels of EPH and those fed the basal diet (p &gt; 0.05). In contrast, the fish fed the EP₂₅ had significantly lower final body weight, weight gain ratio, and specific growth rate as well as higher feed conversion ratio than those fed EPH₅ and EPH₁₀ supplemented diets (p &lt; 0.05). Proximate analysis revealed no significant differences in the moisture content, crude protein content, crude lipid content, ash content, phosphorus level, or gross energy of the fish between the dietary groups (p &gt; 0.05). The amino acid analysis revealed no significant variation in total essential amino acids among the experimental treatments (p &gt; 0.05). However, methionine and alanine levels were lower in fish fed the EPH₅ diet than those fed diets supplemented with higher levels of EPH (p &lt; 0.05). The activity of digestive enzymes significantly improved with increasing EPH supplementation, with trypsin activity peaking in the EPH₁₅ diet. Histomorphological evaluations revealed greater muscle thickness and villus height in the fish fed the EPH supplemented diets compared to those fed the EP₀ and EP₂₅ diets. Gene expression analysis showed significant increases in the mRNA levels of growth hormone and insulin-like growth factor in the fish fed the EPH supplemented diets compared with those in the fish fed the basal diet (p &lt; 0.05). The findings of the present study illustrated that EPH has no adverse effect on the experimental variables compared with fishmeal, indicating its appropriate potential to be used as a viable alternative protein source in the diet of rainbow trout. While substitution of EPH up to 25 % can be effectively utilized to compensate for fishmeal shortages, 10 % EPH substitution is recommended for achieving the best performance and physiological responses in rainbow trout.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103386"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146090850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of two postbiotics (yeast culture and complex probiotics) on growth performance, body composition, liver and intestinal health, and fillet quality of rice field eel (Monopterus albus)","authors":"Jialing Xu ,&nbsp;Jiamin Li ,&nbsp;Ao Luo ,&nbsp;Qilin Hu ,&nbsp;Jiazhen Zhao ,&nbsp;Luyao Xu ,&nbsp;Xiaoxuan Qi ,&nbsp;Shuo Cheng ,&nbsp;Xiaohan Guo ,&nbsp;Tingting Wang ,&nbsp;Mo Peng","doi":"10.1016/j.aqrep.2026.103414","DOIUrl":"10.1016/j.aqrep.2026.103414","url":null,"abstract":"<div><div>This study explored effects of yeast culture (YC) and complex probiotics (CP) on the growth performance, liver-intestinal health and fillet quality of rice field eel (<em>Monopterus albus</em>). 1200 healthy fish with initial weight (23.04 ± 0.04) g were selected and randomly divided into 5 groups (4 replies per group): control group, 0.3 % YC group, 0.6 % YC group, 0.3 % CP group and 0.6 % CP group. Results of 56-d experiment showed that postbiotics could significantly increase final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR) and reduced feed conversion rate (FCR) (<em>P</em> &lt; 0.05). Dietary postbiotics reduced the total lipid content in liver (<em>P</em> &lt; 0.05), increased protein deposition in whole body and muscle (<em>P</em> &lt; 0.05). Dietary postbiotics also improved liver and intestinal antioxidant ability (total superoxide dismutase, catalase, glutathione peroxidase, glutathione) (<em>P</em> &lt; 0.05), immune function (serum acid phosphatase, alkaline phosphatase and liver lysozyme activities) (<em>P</em> &lt; 0.05), and intestinal digestive ability (α-amylase, trypsin) (<em>P</em> &lt; 0.05). Meanwhile, dietary postbiotics significantly enhanced intestinal villi height and mucosal barrier integrity (<em>P</em> &lt; 0.05). High-throughput sequencing revealed that, intestinal microbial probiotics (Firmicutes and Bacteroidetes) abundance increased and pathogenic (Proteobacteria) abundance decreased in the postbiotic-treating group (<em>P</em> &lt; 0.05). It is worth noting that dietary postbiotics could improve muscle quality (<em>P</em> &lt; 0.05), reduce muscle fibers area and diameter (<em>P</em> &lt; 0.05), and promote protein deposition (<em>P</em> &lt; 0.05). In summary, dietary postbiotics can show beneficial effects on growth, liver and intestinal health, and muscle quality in this fish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103414"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatopancreas-ovary crosstalk dynamically modulates fatty acid metabolism during ovarian maturation in Litopenaeus vannamei","authors":"Guanrong Feng ,&nbsp;Sai Wang ,&nbsp;Yong Jiang ,&nbsp;Xuyan Chen ,&nbsp;Xinxin Qi ,&nbsp;Zhenmin Bao ,&nbsp;Yue Sun","doi":"10.1016/j.aqrep.2026.103424","DOIUrl":"10.1016/j.aqrep.2026.103424","url":null,"abstract":"<div><div>The Pacific white shrimp (<em>Litopenaeus vannamei</em>) is an economically significant species in global aquaculture. A critical stage in its reproductive cycle is ovarian development—a complex process closely linked to the metabolic status of the organism. Although the hepatopancreas, as the central metabolic organ, is known to contribute substantially to ovarian development, the regulatory and communicative mechanisms between the two organs remain poorly understood. By integration of histology, proteomics, and transcriptomics analyses, we systematically investigated the physiological changes and the gene regulatory crosstalk between ovary and hepatopancreas during ovarian development in <em>L. vannamei</em>. The expression pattern of the key genes suggested that both hepatopancreas and ovary played critical roles in lipid metabolism. The hepatopancreas exhibited high metabolic activity at early stage, whereas ovarian activity increased progressively throughout the ovarian development stages. By tracing the tissue origins of key regulatory genes, we identified 776 potential ovary-autonomous genes (proteins co-expressed with their mRNAs in the ovary) and 40 hepatopancreas-expressed genes (proteins detected in the ovary whose transcripts likely originate from the hepatopancreas). These analyses revealed a dual-organ regulatory mode whereby interorgan crosstalk is supported by both ovary-autonomous genes, such as enoyl-CoA delta isomerase 2 (<em>ECI2</em>), proliferating cell nuclear antigen (<em>PCNA</em>), polyubiquitin 8 (<em>UBQ8</em>), as well as hepatopancreas-expressed and potential transportable proteins, including vitellogenin (<em>Vg</em>), fatty acid synthase (<em>FASN</em>), stearoyl-CoA desaturase 5 (<em>SCD5</em>), glycine hydroxylmethyltransferase (<em>GHMT</em>), heme-binding protein 2 (<em>HEBP2</em>). Overall, this study significantly enhances our understanding of the synergistic communication between the ovary and hepatopancreas, and provides a scientific basis for future improvement of gonadal regulation techniques in shrimp.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103424"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of thyroxine on embryonic and larval growth and development of Fujian oyster (Crassostrea angulata)","authors":"Ke Du ,&nbsp;Zhongwang Cui ,&nbsp;Ziqiang Han ,&nbsp;Huayong Que","doi":"10.1016/j.aqrep.2026.103408","DOIUrl":"10.1016/j.aqrep.2026.103408","url":null,"abstract":"<div><div>Thyroid hormones (THs) are key regulators of early development and metamorphosis in vertebrates, implying possible developmental roles in invertebrates. Given the importance of embryonic growth and survival to oyster seed quality and aquaculture success, understanding TH function during oyster embryonic development is therefore essential. This study investigated the effects of thyroxine (T4) concentration and exposure duration on the embryonic and larval development of Fujian oyster (<em>Crassostrea angulata</em>)<em>.</em> T4 was administered both by immersing female broodstock and by directly exposing embryos to T4-enriched seawater. Additionally, thiourea was used to inhibit endogenous TH synthesis in broodstock, followed by T4 treatment of their embryos. Each concentration-duration treatment was performed in triplicate, with 0.01 N NaOH serving as the vehicle control. Results showed that low to medium T4 concentrations (1.29 × 10⁻⁹ mol/L, 1.29 × 10⁻⁸ mol/L, 1.29 × 10⁻⁷ mol/L) significantly enhanced the hatching rate and shell length (μm) of <span>D</span>-shaped larvae compared to controls (<em>P</em> &lt; 0.05). In contrast, high T4 concentrations (1.29 × 10⁻⁵ mol/L, 1.29 × 10⁻⁶ mol/L) exhibited significant inhibitory effects (<em>P</em> &lt; 0.05), which intensified with prolonged exposure durations. Using a validated Chemiluminescence immunoassay, results indicated that prolonged exposure may lead to cumulative T4 accumulation, contributing to developmental inhibition. Compared to direct embryonic exposure, the maternal approach potentially appeared to mitigate the inhibitory effects associated with direct high-dose exposure. Additionally, in the 1.29 × 10⁻⁸ mol/L group, an elevated triiodothyronine to T4 ratio was observed from the cleavage to the blastula stage, potentially explaining the observed developmental advantages. In conclusion, this study clarifies the dose- and time-dependent regulatory roles of T4 in early bivalve embryogenesis. While limited to the <span>D</span>-larval stage, these findings provide preliminary theoretical insights into TH dynamics that may inform future aquaculture seed production strategies.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103408"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acceleration of ovarian degeneration by salinity stress is mediated at multiple levels of the hypothalamic-pituitary-gonadal axis and the liver in spotted scat (Scatophagus argus)","authors":"Jie Luo ,&nbsp;Siqin Wang ,&nbsp;Xinghua Lin ,&nbsp;Yi Zhai ,&nbsp;Hongjuan Shi ,&nbsp;Dongneng Jiang ,&nbsp;Huapu Chen ,&nbsp;Tianli Wu ,&nbsp;Siping Deng","doi":"10.1016/j.aqrep.2026.103422","DOIUrl":"10.1016/j.aqrep.2026.103422","url":null,"abstract":"<div><div>The spotted scat (<em>Scatophagus argus</em>) is a coastal fish and that lives in a wide range of salinities. Environmental salinity can affect the reproduction of spotted scat. However, the exact effects of environmental salinity fluctuations on the reproductive regulation in teleosts at the HPG axis and the liver levels remain unclear. Therefore, changes in the gonadosomatic index (GSI) and serum 17β-estradiol (E₂) levels were measured after 20 days of exposure to salinities of 5 ppt (low salinity group, LS), 25 ppt (control group, Ctrl), and 35 ppt (high salinity group, HS) in female spotted scat. The expression of genes associated with reproductive regulation in the hypothalamus (<em>sbgnrh</em> and <em>sgnrh</em>), pituitary (<em>fshβ</em> and <em>lhβ</em>), and ovary (<em>fshr</em> and <em>lhr</em>) was examined. In addition, the genes associated with steroidogenesis and vitellogenesis in the ovary and in the liver were also investigated. The results showed that the GSI decreased significantly, and oocyte degeneration was accelerated with low- and high-salinity stress. ELISA revealed no significant change in estradiol (E₂) levels in the LS and HS groups. qRT-PCR showed a significant decrease in <em>sbgnrh</em> and <em>lhr</em> in the HS group, and in <em>lhβ</em> and <em>lhr</em> in the LS group. However, <em>sgnrh</em>, <em>fshβ,</em> and <em>fshr</em> did not change in either group. Genes associated with steroidogenesis, such as <em>star1</em>, <em>cyp17a1</em>, <em>cyp17a2</em>, and <em>hsd3b1</em>, decreased significantly after acclimation to low and high salinity, however, <em>hsd17b14</em> was increased. Vitellogenesis-associated genes, such as estrogen receptors (<em>erα</em> and <em>erβ2</em>) in the ovary, decreased, while vitellogenin (<em>vtgAa</em>, <em>vtgAb</em>, and <em>vtgc</em>) increased in the liver. These results suggest that environmental salinity stress profoundly affects the reproductive physiology of spotted scat by disrupting the HPG axis signaling, steroidogenic pathways, and vitellogenesis. This paper provides a comprehensive insight into the impact of environmental salinity stress on reproductive regulation in spotted scat.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103422"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating automated cytometry and microbial surveillance in a public marine aquarium: Insights from a one-year study in Monaco","authors":"Emmanuelle Boisard ,&nbsp;Rita Di Martino ,&nbsp;Stéphanie Orengo ,&nbsp;Olivier Brunel ,&nbsp;Luigino Grasso ,&nbsp;Jérémy Senouillet ,&nbsp;Alice Klein ,&nbsp;Tatiana Proust ,&nbsp;Fiorella Lucarini ,&nbsp;Davide Staedler","doi":"10.1016/j.aqrep.2026.103457","DOIUrl":"10.1016/j.aqrep.2026.103457","url":null,"abstract":"<div><div>This study presents a one-year microbiological monitoring campaign conducted at the Oceanographic Institute of Monaco to evaluate microbial dynamics within a large public marine aquarium. The approach combined automated near-real-time flow cytometry, culture-based analyses, species identification, and antibiotic susceptibility testing. Continuous bacterial enumeration using the BactoSense® cytometer enabled high-resolution tracking of intact bacterial counts (ICC) across five hydraulic sectors, from seawater intake to exhibition tanks. The results revealed pronounced spatial gradients, with mean ICC values decreasing from 1.4 × 10⁵ cells mL⁻¹ at intake to below 1.3 × 10⁴ cells mL⁻¹ in internal tanks, confirming the high efficiency of UV-C disinfection and filtration systems. Seasonal analysis showed clear fluctuations at the intake, with peaks in spring and early summer associated with increased biological activity in the surrounding Mediterranean environment. Sector-specific variations indicated that hydraulic configuration and residence time influenced microbial stabilization, with localized differences in UV efficiency detected through near-real-time monitoring. Complementary bacterial identification revealed the presence of several opportunistic taxa, including <em>V. fortis</em>, <em>V. harveyi</em>, and <em>Shewanella</em> spp., typical of recirculating seawater systems. Antibiotic susceptibility testing revealed heterogeneous resistance profiles, with β-lactam resistance observed in several <em>Vibrio</em> isolates and multidrug resistance detected in <em>S. algae</em> and <em>V. jasicida</em>. The integration of biosensing, microbial identification, and resistance profiling provides an operational framework for evidence-based microbial surveillance in marine aquaria. These findings highlight the potential of automated near-real-time cytometry to enhance preventive management, support targeted interventions, and ensure microbiological stability in closed aquatic ecosystems.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103457"},"PeriodicalIF":3.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}