Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2023.05.122
Semiha Nur Ozkaya, Tuba Keskin, Ç. Tekin, S. Tekin, A. Beytur
Objective: Inflammation is a process associated with the development and progression of cancer. Desloratadine (DES) and Trimebutin (TMB) are anti-inflammatory agents used in the treatment of various diseases. This study aimed to investigate the antitumor effects of DES and TMB, which exhibit anti-inflammatory effects, on different human cancer cell lines. Materials and Methods: In this study, human prostate (LNCaP), ovarian (A2780), breast (MCF-7) and colon cancer (Caco-2) cell lines were treated with DES and TMB at concentrations of 1, 5, 25, 50 and 100 µM. Cells were treated with the compounds for 6, 12, and 24 hours, and the change in cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The inhibitory concentration 50 (LogIC50) values of the compounds were calculated using GraphPad Prism 8 software based on cell viability results. The genotoxic effects of the compounds on cells were determined using the comet assay. Group comparisons were performed using the Kruskal-Wallis H test and p<0.05 was considered significant. Results: Exposure of LNCaP, A2780, MCF-7, and Caco-2 cells to DES and TMB agents for 6, 12 and 24 hours significantly reduced cell viability (p≤0.05). According to the comet assay results, DES and TMB caused significant DNA damage in the cell lines (p≤0.05). Conclusion: The study results demonstrate that DES and TMB, which have anti-inflammatory effects, exert cytotoxic effects by inducing DNA damage in cancer cells.
{"title":"Investigation of the Antitumor Effects of Anti-inflammatory Desloratadine and Trimebutine on Different Types of Human Cancer Cells: An In Vitro study","authors":"Semiha Nur Ozkaya, Tuba Keskin, Ç. Tekin, S. Tekin, A. Beytur","doi":"10.5455/annalsmedres.2023.05.122","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.05.122","url":null,"abstract":"Objective: Inflammation is a process associated with the development and progression of cancer. Desloratadine (DES) and Trimebutin (TMB) are anti-inflammatory agents used in the treatment of various diseases. This study aimed to investigate the antitumor effects of DES and TMB, which exhibit anti-inflammatory effects, on different human cancer cell lines. Materials and Methods: In this study, human prostate (LNCaP), ovarian (A2780), breast (MCF-7) and colon cancer (Caco-2) cell lines were treated with DES and TMB at concentrations of 1, 5, 25, 50 and 100 µM. Cells were treated with the compounds for 6, 12, and 24 hours, and the change in cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The inhibitory concentration 50 (LogIC50) values of the compounds were calculated using GraphPad Prism 8 software based on cell viability results. The genotoxic effects of the compounds on cells were determined using the comet assay. Group comparisons were performed using the Kruskal-Wallis H test and p<0.05 was considered significant. Results: Exposure of LNCaP, A2780, MCF-7, and Caco-2 cells to DES and TMB agents for 6, 12 and 24 hours significantly reduced cell viability (p≤0.05). According to the comet assay results, DES and TMB caused significant DNA damage in the cell lines (p≤0.05). Conclusion: The study results demonstrate that DES and TMB, which have anti-inflammatory effects, exert cytotoxic effects by inducing DNA damage in cancer cells.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90918909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2023.01.028
M. K., Hakan Hakkoymaz
{"title":"The Role of Delta Neutrophil Index (Immature Granulocyte) and Hematological Markers in the Diagnosis of Pediatric Acute Appendicitis","authors":"M. K., Hakan Hakkoymaz","doi":"10.5455/annalsmedres.2023.01.028","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.01.028","url":null,"abstract":"<jats:p />","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79294871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2022.12.395
A. M. Res, Ahmet Ziya Şahi̇n, Ç. Demiroğlu
{"title":"The Effect of Natural Antioxidant Levels in Pregnancy on Birth Weight","authors":"A. M. Res, Ahmet Ziya Şahi̇n, Ç. Demiroğlu","doi":"10.5455/annalsmedres.2022.12.395","DOIUrl":"https://doi.org/10.5455/annalsmedres.2022.12.395","url":null,"abstract":"<jats:p />","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75877130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2023.02.054
Ayhan rk, A. Ayaz
Introduction: Bacteremia and bile duct infection following percutaneous transhepatic biliary drainage (PTBD) are severe complications. Thus, the aim of this study was to investigate the risk factors of mortality and to evaluate the differences between blood and biliary drainage culture positive patients after PTBD. Materials and Methods: This is a retrospective study of 90 patients without any signs of infection prior to the PTBD procedures. Of these patients, we further divided them into three groups based on culture results: 1) first group was only bacteremic, 2) second group was only bile drainage culture-positive, and 3) third group was both bacteremic and bile drainage culture-positive. We compared clinical and laboratory parameters between these three groups and also evaluated the culture results in a high-resistance setting. Results: There were 90 patients who developed bacteremia, bile duct infections or both. In the laboratory findings, bacteremic patients showed significantly higher serum level of alanine aminotransferase (ALT) (p=0.001). Elevated neutrophil counts, lipase levels and carbapenem-resistant isolates were found to be the independent risk factors for 30-day mortality after PTBD. (p=0.01, p=0.01, and p=0.04, respectively). Gram-negative organisms were present in 71.2% of the cases; Escherichia coli was the most common species (28.1%) followed by Enterococcus faecium (18.1%), Klebsiella pneumoniae (15.8%) and Pseudomonas aeruginosa (9.9%). 51 (41.8%) of the cultured gram-negative bacteria were extended spectrum beta-lactamases positive and carbapenem resistance was found in 27 (15.8%) isolates. Conclusion: Elevated ALT level was only difference between three groups. Therefore, bacteremic and bile culture positive patients should be treated with the same care. Elevated neutrophil counts, lipase levels and carbapenem-resistant isolates were the independent risk factors for mortality. Finally, the choice of antibiotic prophylaxis should be reviewed according to the antimicrobial resistance profiles.
{"title":"Evaluation of Blood and Biliary Drainage Cultures and Mortality after Percutaneous Transhepatic Biliary Drainage Procedure","authors":"Ayhan rk, A. Ayaz","doi":"10.5455/annalsmedres.2023.02.054","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.02.054","url":null,"abstract":"Introduction: Bacteremia and bile duct infection following percutaneous transhepatic biliary drainage (PTBD) are severe complications. Thus, the aim of this study was to investigate the risk factors of mortality and to evaluate the differences between blood and biliary drainage culture positive patients after PTBD. Materials and Methods: This is a retrospective study of 90 patients without any signs of infection prior to the PTBD procedures. Of these patients, we further divided them into three groups based on culture results: 1) first group was only bacteremic, 2) second group was only bile drainage culture-positive, and 3) third group was both bacteremic and bile drainage culture-positive. We compared clinical and laboratory parameters between these three groups and also evaluated the culture results in a high-resistance setting. Results: There were 90 patients who developed bacteremia, bile duct infections or both. In the laboratory findings, bacteremic patients showed significantly higher serum level of alanine aminotransferase (ALT) (p=0.001). Elevated neutrophil counts, lipase levels and carbapenem-resistant isolates were found to be the independent risk factors for 30-day mortality after PTBD. (p=0.01, p=0.01, and p=0.04, respectively). Gram-negative organisms were present in 71.2% of the cases; Escherichia coli was the most common species (28.1%) followed by Enterococcus faecium (18.1%), Klebsiella pneumoniae (15.8%) and Pseudomonas aeruginosa (9.9%). 51 (41.8%) of the cultured gram-negative bacteria were extended spectrum beta-lactamases positive and carbapenem resistance was found in 27 (15.8%) isolates. Conclusion: Elevated ALT level was only difference between three groups. Therefore, bacteremic and bile culture positive patients should be treated with the same care. Elevated neutrophil counts, lipase levels and carbapenem-resistant isolates were the independent risk factors for mortality. Finally, the choice of antibiotic prophylaxis should be reviewed according to the antimicrobial resistance profiles.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79064724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2022.12.398
S. Ediz, U. Soylemez, Başak Atalay
{"title":"Approach to the intracranial aneurysm in patients with autosomal dominant polycystic kidney; Are current radiological screening recommendations applied?","authors":"S. Ediz, U. Soylemez, Başak Atalay","doi":"10.5455/annalsmedres.2022.12.398","DOIUrl":"https://doi.org/10.5455/annalsmedres.2022.12.398","url":null,"abstract":"<jats:p />","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75795621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2022.11.332
smail ndar
{"title":"Incidental cause of mild hyperglycemia in children: genetic, clinical, and follow-up features of Glucokinase-MODY diabetes","authors":"smail ndar","doi":"10.5455/annalsmedres.2022.11.332","DOIUrl":"https://doi.org/10.5455/annalsmedres.2022.11.332","url":null,"abstract":"<jats:p />","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"93 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88383275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2023.08.207
Tu l
Aim: The aim of this study was to determine the anticancer activity of royal jelly (RJ) and Aloe vera (AVE) separately and in combination on human non-small cell lung cancer (A549) and colorectal adenocarcinoma cells (HT29) and to investigate whether the side effects of AVE can be ameliorated by RJ. Materials and Methods: The antiproliferative activity of RJ, AVE, and the combination of AVE and RJ was performed in vitro on A549 and HT29 cells using a Real-Time Cell Analyzer (xCELLigence). To determine the mechanisms underlying the antiproliferative activity, cell apoptosis was performed by flow cytometry using the annexin V-FITC/PI apoptosis detection kit, while cell migration was determined by wound healing assay. Results: Treatment with varying concentrations of AVE, RJ, and the combination of AVE and RJ was concluded to have dose-dependent antiproliferative activity and that the AVE-RJ combination induced 41% of HT29 and 11.79% of A549 early apoptosis (p<0.05). However, the inhibition effect of the combination of AVE and RJ on cancer cell migration was not detected. Conclusion: Taken together, our results showed that the AVE-RJ combination could be a promising therapeutic combination in A549 and HT29 by inducing apoptosis. The obtained results revealed that the AVE (40 µg/mL) and RJ (300 mg/mL) combination in the A549 cell line exhibited earlier antiproliferative activity than the 40 µg/mL AVE. Also, it has been found that the antiproliferative activity of AVE is enhanced when used in combination with RJ. Keywords: Aloe vera; Royal jelly; Non-small cell lung cancer; Colorectal adenocarcinoma; Real-time cell analyzer/xCELLigence); Wound healing assay.
{"title":"Enhanced anticancer potency of Aloe vera in combination with Royal jelly in non-small cell lung cancer and colorectal adenocarcinoma","authors":"Tu l","doi":"10.5455/annalsmedres.2023.08.207","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.08.207","url":null,"abstract":"Aim: The aim of this study was to determine the anticancer activity of royal jelly (RJ) and Aloe vera (AVE) separately and in combination on human non-small cell lung cancer (A549) and colorectal adenocarcinoma cells (HT29) and to investigate whether the side effects of AVE can be ameliorated by RJ. Materials and Methods: The antiproliferative activity of RJ, AVE, and the combination of AVE and RJ was performed in vitro on A549 and HT29 cells using a Real-Time Cell Analyzer (xCELLigence). To determine the mechanisms underlying the antiproliferative activity, cell apoptosis was performed by flow cytometry using the annexin V-FITC/PI apoptosis detection kit, while cell migration was determined by wound healing assay. Results: Treatment with varying concentrations of AVE, RJ, and the combination of AVE and RJ was concluded to have dose-dependent antiproliferative activity and that the AVE-RJ combination induced 41% of HT29 and 11.79% of A549 early apoptosis (p<0.05). However, the inhibition effect of the combination of AVE and RJ on cancer cell migration was not detected. Conclusion: Taken together, our results showed that the AVE-RJ combination could be a promising therapeutic combination in A549 and HT29 by inducing apoptosis. The obtained results revealed that the AVE (40 µg/mL) and RJ (300 mg/mL) combination in the A549 cell line exhibited earlier antiproliferative activity than the 40 µg/mL AVE. Also, it has been found that the antiproliferative activity of AVE is enhanced when used in combination with RJ. Keywords: Aloe vera; Royal jelly; Non-small cell lung cancer; Colorectal adenocarcinoma; Real-time cell analyzer/xCELLigence); Wound healing assay.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134979953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2023.08.196
P Akhanl, Sema en, Hakan er, Muhammed l, Salim lu, zcan Erel, Erman akal
Objectives: Aim of this study is to analyze the correlation between thiol/disulfide homeostasis (TDH) and papillary thyroid carcinoma (PTCA) and to reveal the possible impact of TDH on the development of PTCA. Material and Methods: Preoperative venous blood sampling was obtained from the patients having applied to our hospital due to euthyroid nodular goiter and detected with suspected malignancy or malignant cytology in FNAB, according to the BETHESDA system. Those whose postoperative histopathological results proved the presence of PTCA were involved in this study. Healthy volunteers who were without any thyroid nodules and thyroid dysfunction. Results: The number of participants in the patient and control groups was 34 and 101, respectively. Compared to the control group, native thiol (μmol/L), total thiol (μmol/L), and native thiol/total thiol × 100 values were ascertained to be lower in the patient group with PTCA; however, these values were not statistically significant. Disulfide, disulfide/native thiol × 100, and disulfide/total thiol × 100 values were detected to be lower in the patient group with PTCA, but not statistically significant. When the patients with PTCA were assessed within themselves, TDH parameters were not associated with the PTCA subtype, the number of foci, the presence of lymphovascular invasion, and the presence of metastatic lap. Conclusion: Considering the parameters of PTCA and TDH, the results were not statistically significant. And besides postoperative histopathological findings such as the PTCA subtype, the number of foci, the presence of lymphovascular invasion, and presence of metastatic lap were not correlated with THD parameters.
{"title":"The Evaluation of Dynamic Thiol/Disulfide Homeostasis in Papillary Thyroid Carcinoma","authors":"P Akhanl, Sema en, Hakan er, Muhammed l, Salim lu, zcan Erel, Erman akal","doi":"10.5455/annalsmedres.2023.08.196","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.08.196","url":null,"abstract":"Objectives: Aim of this study is to analyze the correlation between thiol/disulfide homeostasis (TDH) and papillary thyroid carcinoma (PTCA) and to reveal the possible impact of TDH on the development of PTCA. Material and Methods: Preoperative venous blood sampling was obtained from the patients having applied to our hospital due to euthyroid nodular goiter and detected with suspected malignancy or malignant cytology in FNAB, according to the BETHESDA system. Those whose postoperative histopathological results proved the presence of PTCA were involved in this study. Healthy volunteers who were without any thyroid nodules and thyroid dysfunction. Results: The number of participants in the patient and control groups was 34 and 101, respectively. Compared to the control group, native thiol (μmol/L), total thiol (μmol/L), and native thiol/total thiol × 100 values were ascertained to be lower in the patient group with PTCA; however, these values were not statistically significant. Disulfide, disulfide/native thiol × 100, and disulfide/total thiol × 100 values were detected to be lower in the patient group with PTCA, but not statistically significant. When the patients with PTCA were assessed within themselves, TDH parameters were not associated with the PTCA subtype, the number of foci, the presence of lymphovascular invasion, and the presence of metastatic lap. Conclusion: Considering the parameters of PTCA and TDH, the results were not statistically significant. And besides postoperative histopathological findings such as the PTCA subtype, the number of foci, the presence of lymphovascular invasion, and presence of metastatic lap were not correlated with THD parameters.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135159960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2023.01.014
eyda Can, Berna Dalcal, Hanife Durgun
Aim: This research was carried out to develop a measurement instrument to determine the innovative behaviors of nurses and midwives. Materials and Methods: This study, which is of a methodological nature was applied with 216 nurses and midwives between 01.06.2021-31.09.2021. The item pool of the scale was created by the researchers with the literature as twenty-three items in a five-point Likert type and submitted to the opinions of twelve specialists. Results: The three-factor scale structure designed by making explanatory and confirmatory factor analysis was confirmed with the validity and reliability. According to the analysis results; the scale consisted of three sub-dimensions (managerial and personal; physical and occupational; organizational and environmental) and 15 items. It was founded that the content validity index of the scale items was 0.468-0.851; factor loads were 0.510-0.871; item-total score correlations were 0.467-0.768, and the Cronbach's alpha reliability coefficient of the sub-dimensions ranged between 0.703-0.876 and it was 0.912 for the whole scale. Conclusion: As a result of the validity and reliability analyzes of the Attitude Scale towards Innovative Behaviors of Nurses and Midwives, it was founded that it can be used as a valid and reliable scale.
{"title":"Validity and reliability of innovative behaviors of nurses and midwives scale: A scale development study","authors":"eyda Can, Berna Dalcal, Hanife Durgun","doi":"10.5455/annalsmedres.2023.01.014","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.01.014","url":null,"abstract":"Aim: This research was carried out to develop a measurement instrument to determine the innovative behaviors of nurses and midwives. Materials and Methods: This study, which is of a methodological nature was applied with 216 nurses and midwives between 01.06.2021-31.09.2021. The item pool of the scale was created by the researchers with the literature as twenty-three items in a five-point Likert type and submitted to the opinions of twelve specialists. Results: The three-factor scale structure designed by making explanatory and confirmatory factor analysis was confirmed with the validity and reliability. According to the analysis results; the scale consisted of three sub-dimensions (managerial and personal; physical and occupational; organizational and environmental) and 15 items. It was founded that the content validity index of the scale items was 0.468-0.851; factor loads were 0.510-0.871; item-total score correlations were 0.467-0.768, and the Cronbach's alpha reliability coefficient of the sub-dimensions ranged between 0.703-0.876 and it was 0.912 for the whole scale. Conclusion: As a result of the validity and reliability analyzes of the Attitude Scale towards Innovative Behaviors of Nurses and Midwives, it was founded that it can be used as a valid and reliable scale.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"169 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135839407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/annalsmedres.2023.08.186
Esen ekli, Asl du, Dilcan Kotan, Asuman zkan
Aim: In order to survive, human cells must adapt to changes in the oxygen level of their environment. Many endogenous mechanisms operate in hypoxia. Hypoxia-inducible factor 1 (HIF-1) is a transcriptional protein involved in the head of the cascade that regulates the response of brain and other tissues to hypoxia. HIF-1 alpha (HIF-1α) is the main molecule that responds to hypoxia and is an important marker of cerebral hypoperfusion. Materials and Methods: 39 acute stroke patients aged between 18-80 years and 38 control patients included in our study. We aimed to examine the serum HIF-1α levels in early period of acute ischemic stroke. We also aimed to evaluate its usefulness in determining tissue damage due to hypoxia and the prognosis of the disease. Results: Serum HIF-1α levels were higher in acute ischemic stroke patients compared to the control group (p= 0.000). There was no significant correlation between infarct volumes, NHISS scores, blood pressure and HIF-1α values of the stroke patients. Conclusion: HIF-1α could be an important biomarker in detecting cerebral hypoxia. It is possible to study this in human peripheral blood. Its usability in stroke may become widespread with studies on its therapeutic target.
{"title":"Serum hypoxia-inducible factor 1 alpha levels in stroke","authors":"Esen ekli, Asl du, Dilcan Kotan, Asuman zkan","doi":"10.5455/annalsmedres.2023.08.186","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.08.186","url":null,"abstract":"Aim: In order to survive, human cells must adapt to changes in the oxygen level of their environment. Many endogenous mechanisms operate in hypoxia. Hypoxia-inducible factor 1 (HIF-1) is a transcriptional protein involved in the head of the cascade that regulates the response of brain and other tissues to hypoxia. HIF-1 alpha (HIF-1α) is the main molecule that responds to hypoxia and is an important marker of cerebral hypoperfusion. Materials and Methods: 39 acute stroke patients aged between 18-80 years and 38 control patients included in our study. We aimed to examine the serum HIF-1α levels in early period of acute ischemic stroke. We also aimed to evaluate its usefulness in determining tissue damage due to hypoxia and the prognosis of the disease. Results: Serum HIF-1α levels were higher in acute ischemic stroke patients compared to the control group (p= 0.000). There was no significant correlation between infarct volumes, NHISS scores, blood pressure and HIF-1α values of the stroke patients. Conclusion: HIF-1α could be an important biomarker in detecting cerebral hypoxia. It is possible to study this in human peripheral blood. Its usability in stroke may become widespread with studies on its therapeutic target.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134981039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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