Pub Date : 2022-06-01DOI: 10.21608/aps.2022.117937.1078
Miriam F. Ayad, A. El-Kosasy, Ahmed I. Sheta, S. Tawakkol, A. Fayed
Large molecules pass the biological membranes through accessory elements, either carriers (ionophores) and/or water channels. Ionophores shield the charge of the target ion, enabling it to cross the membrane to be released on the other side. This may consume time and some huge structures need a bulky ionophore. However, water channels enable the passage of many ions at a high rate without the need for carriers that may limit the ion transfer rate. Simulation of living cell membrane in engulfing large molecules through channels is a very interesting mechanism to enhance permselectivity of fabricated polyvinyl chloride (PVC) membrane sensors. Green synthesis of carbonaceous nanoparticles to play this role was fulfilled in nanocarbon dots (CD). The polymerization of β-cyclodextrin with acrylates, either imprinted with the target molecule (in molecularly imprinted polymer, MIP) or not (in non-imprinted polymer, NIP) provided a good enhancement of binding selectivity, in comparison with β-cyclodextrin alone as ionophore. Application of both inventions in the fabrication of three sensors (1; CD/βCD, 2; CD/NIP, and 3; CD/MIP) enabled trace analysis of azoxystrobin fungicide in ranges (10 -11 -10 -5 , 10 -13 -10 -4 and 10 -13 -10 -4 M), respectively, with high selectivity.
{"title":"Comparison between molecularly imprinted polymers and β-cyclodextrin as ionophores, in presence of nano carbon dots water channels and application in marketed product and environmental samples","authors":"Miriam F. Ayad, A. El-Kosasy, Ahmed I. Sheta, S. Tawakkol, A. Fayed","doi":"10.21608/aps.2022.117937.1078","DOIUrl":"https://doi.org/10.21608/aps.2022.117937.1078","url":null,"abstract":"Large molecules pass the biological membranes through accessory elements, either carriers (ionophores) and/or water channels. Ionophores shield the charge of the target ion, enabling it to cross the membrane to be released on the other side. This may consume time and some huge structures need a bulky ionophore. However, water channels enable the passage of many ions at a high rate without the need for carriers that may limit the ion transfer rate. Simulation of living cell membrane in engulfing large molecules through channels is a very interesting mechanism to enhance permselectivity of fabricated polyvinyl chloride (PVC) membrane sensors. Green synthesis of carbonaceous nanoparticles to play this role was fulfilled in nanocarbon dots (CD). The polymerization of β-cyclodextrin with acrylates, either imprinted with the target molecule (in molecularly imprinted polymer, MIP) or not (in non-imprinted polymer, NIP) provided a good enhancement of binding selectivity, in comparison with β-cyclodextrin alone as ionophore. Application of both inventions in the fabrication of three sensors (1; CD/βCD, 2; CD/NIP, and 3; CD/MIP) enabled trace analysis of azoxystrobin fungicide in ranges (10 -11 -10 -5 , 10 -13 -10 -4 and 10 -13 -10 -4 M), respectively, with high selectivity.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84349789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/aps.2022.143040.1092
Hagar Elmenshawy, H. Farouk, N. Sabri, M. Ahmed
The objective of our study was to investigate the impact of pharmaceutical care services on the detection and resolution of drug-related problems (DRPs) in rheumatoid arthritis (RA) patients. It was a randomized controlled study in which 60 eligible patients were recruited and randomly assigned to either an intervention (N = 30) or a control group (N = 30). The intervention group received pharmaceutical care services including management of drug-related problems (DRPs) in addition to standard care. Patients in the control group received only standard care. Both groups were evaluated for DRPs, disease activity, functional disability, adherence, quality of life, and laboratory tests that include erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) at baseline and after 6 months. After 6 months, a significant difference in DRPs, Morisky medication adherence scale (MMAS-8), number of adverse drug reactions, and administration errors were noted between the intervention and control groups. A significant reduction was observed in disease activity score 28 (DAS28), health assessment questionnaire (HAQ), RA quality of life (RAQoL) score, ESR, and CRP in the intervention group when compared to the control group. In conclusion, the introduction of pharmaceutical care services in RA patient treatment protocol effectively resulted in an improvement in the detection and prevention of drug-related problems. Moreover, these professional pharmaceutical practices showed a significant reduction in DAS28, HAQ, and RAQoL scores indicating a decrease in disease activity, and functional disability with an improvement in patient adherence and quality of life.
{"title":"The Impact of Pharmaceutical Care Services on Patients with Active Rheumatoid Arthritis: A Randomized Controlled Study","authors":"Hagar Elmenshawy, H. Farouk, N. Sabri, M. Ahmed","doi":"10.21608/aps.2022.143040.1092","DOIUrl":"https://doi.org/10.21608/aps.2022.143040.1092","url":null,"abstract":"The objective of our study was to investigate the impact of pharmaceutical care services on the detection and resolution of drug-related problems (DRPs) in rheumatoid arthritis (RA) patients. It was a randomized controlled study in which 60 eligible patients were recruited and randomly assigned to either an intervention (N = 30) or a control group (N = 30). The intervention group received pharmaceutical care services including management of drug-related problems (DRPs) in addition to standard care. Patients in the control group received only standard care. Both groups were evaluated for DRPs, disease activity, functional disability, adherence, quality of life, and laboratory tests that include erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) at baseline and after 6 months. After 6 months, a significant difference in DRPs, Morisky medication adherence scale (MMAS-8), number of adverse drug reactions, and administration errors were noted between the intervention and control groups. A significant reduction was observed in disease activity score 28 (DAS28), health assessment questionnaire (HAQ), RA quality of life (RAQoL) score, ESR, and CRP in the intervention group when compared to the control group. In conclusion, the introduction of pharmaceutical care services in RA patient treatment protocol effectively resulted in an improvement in the detection and prevention of drug-related problems. Moreover, these professional pharmaceutical practices showed a significant reduction in DAS28, HAQ, and RAQoL scores indicating a decrease in disease activity, and functional disability with an improvement in patient adherence and quality of life.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81191333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/aps.2022.145247.1093
Asmaa Shean, Gamal H Abdel Rahman, N. Sabri, M. Adel
Patient satisfaction is a significant indicator of high-quality pharmacy service delivery, and consequently, assessment of customer satisfaction is a crucial technique for identifying and filling gaps in pharmacy service delivery. Evaluation of chronic obstructive pulmonary disease (COPD) patients‟ satisfaction with pharmaceutical care services in the chest department outpatient clinic, Ain Shams University Hospitals, Cairo, Egypt, a cross-sectional study utilizing a structurally designed questionnaire was conducted to measure COPD patient satisfaction with pharmaceutical care services. The questionnaire was filled out by thirty COPD patients, after 12 weeks of providing pharmaceutical care services by the researcher (clinical pharmacist). It was a self-administered survey constituted of two main parts; the first one included the patients' demographic data, and the second part included several domains about various pharmaceutical care services. The questionnaire provided a 5-point Likert response scale system used in Arabic and English language. Most of the patients‟ responses were in the “Good” and the “Very good” categories, and the greater age median of the respondents was associated with a better rating of the services. Providing information about the side effects of the medications and the inhaler technique appeared to enhance patients‟ satisfaction. The satisfaction of COPD patients towards the services provided by the clinical pharmacist in the chest outpatient clinic is only intermediate, the older aged patients seemed to be more satisfied with the services provided than their younger counterparts. Efforts should be performed to increase the patient's satisfaction with the clinical pharmacy services.
{"title":"Assessment of Chronic Obstructive Pulmonary Disease Patient’s Satisfaction with The Provided Pharmaceutical Care Services","authors":"Asmaa Shean, Gamal H Abdel Rahman, N. Sabri, M. Adel","doi":"10.21608/aps.2022.145247.1093","DOIUrl":"https://doi.org/10.21608/aps.2022.145247.1093","url":null,"abstract":"Patient satisfaction is a significant indicator of high-quality pharmacy service delivery, and consequently, assessment of customer satisfaction is a crucial technique for identifying and filling gaps in pharmacy service delivery. Evaluation of chronic obstructive pulmonary disease (COPD) patients‟ satisfaction with pharmaceutical care services in the chest department outpatient clinic, Ain Shams University Hospitals, Cairo, Egypt, a cross-sectional study utilizing a structurally designed questionnaire was conducted to measure COPD patient satisfaction with pharmaceutical care services. The questionnaire was filled out by thirty COPD patients, after 12 weeks of providing pharmaceutical care services by the researcher (clinical pharmacist). It was a self-administered survey constituted of two main parts; the first one included the patients' demographic data, and the second part included several domains about various pharmaceutical care services. The questionnaire provided a 5-point Likert response scale system used in Arabic and English language. Most of the patients‟ responses were in the “Good” and the “Very good” categories, and the greater age median of the respondents was associated with a better rating of the services. Providing information about the side effects of the medications and the inhaler technique appeared to enhance patients‟ satisfaction. The satisfaction of COPD patients towards the services provided by the clinical pharmacist in the chest outpatient clinic is only intermediate, the older aged patients seemed to be more satisfied with the services provided than their younger counterparts. Efforts should be performed to increase the patient's satisfaction with the clinical pharmacy services.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76186594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/aps.2022.120317.1079
A. Salem, Haidy A. Gad, E. Al-sayed, S. El-Ahmady
{"title":"Recent trends on the quality assessment of Pomegranate: A detailed review.","authors":"A. Salem, Haidy A. Gad, E. Al-sayed, S. El-Ahmady","doi":"10.21608/aps.2022.120317.1079","DOIUrl":"https://doi.org/10.21608/aps.2022.120317.1079","url":null,"abstract":"","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90914261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/aps.2022.124160.1082
R. Naji, Abdallah H. Fathy, M. Bashandy
The aim is to study the possible protection of fig fruit extract with olive oil and date-palm fruit extract (FOD) as natural antioxidants in decreasing the subchronic toxicity hazards of silicon oxide nanoparticles (SiO 2 NPs), aluminum oxide nanoparticles (Al 2 O 3 NPs), or zinc oxide nanoparticles (ZnONPs) in male rats treated for 75 days. We used 80 male Wistar rats distributed into 8 groups (n = 10) according to the experiment. We used various antioxidant treatments at their recommended antioxidant doses. All nanoparticles were given orally and daily for 75 days at doses of 100 mg/kg. The oral administration of different nanoparticles (NPs) alone led to dramatic histopathological features, a significant increase in the levels of the TBARS, tumor suppressor p53, and inflammatory markers (TNF-α and IL-6) in the renal tissue. In addition, serum kidney function parameters elevated significantly in NPs treated groups compared with the control group. On the other hand, the renal TAC, GSH, SOD, and TBARS lowered significantly in the renal tissue of rats administered with different NPs compared to the control group. The FOD-NPs-treated groups recorded significantly reduced nephrotoxicity effects as compared to the groups treated with NPs alone. In Conclusion , administration of FOD provides considerable protective effects against NPs-induced subchronic nephrotoxicity in male Wistar rats.
{"title":"The protective role of some natural antioxidants against some nanoparticles-induced subchronic nephrotoxicity in Wistar rats","authors":"R. Naji, Abdallah H. Fathy, M. Bashandy","doi":"10.21608/aps.2022.124160.1082","DOIUrl":"https://doi.org/10.21608/aps.2022.124160.1082","url":null,"abstract":"The aim is to study the possible protection of fig fruit extract with olive oil and date-palm fruit extract (FOD) as natural antioxidants in decreasing the subchronic toxicity hazards of silicon oxide nanoparticles (SiO 2 NPs), aluminum oxide nanoparticles (Al 2 O 3 NPs), or zinc oxide nanoparticles (ZnONPs) in male rats treated for 75 days. We used 80 male Wistar rats distributed into 8 groups (n = 10) according to the experiment. We used various antioxidant treatments at their recommended antioxidant doses. All nanoparticles were given orally and daily for 75 days at doses of 100 mg/kg. The oral administration of different nanoparticles (NPs) alone led to dramatic histopathological features, a significant increase in the levels of the TBARS, tumor suppressor p53, and inflammatory markers (TNF-α and IL-6) in the renal tissue. In addition, serum kidney function parameters elevated significantly in NPs treated groups compared with the control group. On the other hand, the renal TAC, GSH, SOD, and TBARS lowered significantly in the renal tissue of rats administered with different NPs compared to the control group. The FOD-NPs-treated groups recorded significantly reduced nephrotoxicity effects as compared to the groups treated with NPs alone. In Conclusion , administration of FOD provides considerable protective effects against NPs-induced subchronic nephrotoxicity in male Wistar rats.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84267158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/aps.2022.126565.1083
Mai I. Shahin, Asmaa I. AbdAlhafiz, K. Abouzid, Wesam E Mehanna
The intractable emergence of bacterial resistance worldwide urges the need for developing new effective antimicrobial agents to face such virulent strains. Based on the reported effectiveness of both chalcones and benzimidazoles on different bacterial strains including MRSA (Methicillin-Resistant Staphylococcus aureus ), a hybridization strategy to design a scaffold comprising both moieties was achieved and a series of novel chalcone-benzimidazole based compounds were synthesized and tested for their antimicrobial activity. This was performed against Staphylococcus aureus as a representative of Gram-positive bacteria and Escherichia coli representing Gram-negative bacteria. The satisfactory activity shown against Staphylococcus aureus was a great promoter to test the compounds versus linezolid-resistant MRSA. Promising antimicrobial activity against MRSA was observed for three compounds 3b , 3c, and 3e where compound 3e is the most active showing MIC of 3.05 mg/ml. Modest chemical modification revealed a great difference in the activity profile which is intriguing to accomplish further modifications.
{"title":"Synthesis, Biological Evaluation and In-silico ADME Study of Novel Hybridized Benzimidazole-Chalcone Compounds with Potential Antimicrobial Activity","authors":"Mai I. Shahin, Asmaa I. AbdAlhafiz, K. Abouzid, Wesam E Mehanna","doi":"10.21608/aps.2022.126565.1083","DOIUrl":"https://doi.org/10.21608/aps.2022.126565.1083","url":null,"abstract":"The intractable emergence of bacterial resistance worldwide urges the need for developing new effective antimicrobial agents to face such virulent strains. Based on the reported effectiveness of both chalcones and benzimidazoles on different bacterial strains including MRSA (Methicillin-Resistant Staphylococcus aureus ), a hybridization strategy to design a scaffold comprising both moieties was achieved and a series of novel chalcone-benzimidazole based compounds were synthesized and tested for their antimicrobial activity. This was performed against Staphylococcus aureus as a representative of Gram-positive bacteria and Escherichia coli representing Gram-negative bacteria. The satisfactory activity shown against Staphylococcus aureus was a great promoter to test the compounds versus linezolid-resistant MRSA. Promising antimicrobial activity against MRSA was observed for three compounds 3b , 3c, and 3e where compound 3e is the most active showing MIC of 3.05 mg/ml. Modest chemical modification revealed a great difference in the activity profile which is intriguing to accomplish further modifications.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90878724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/aps.2022.106854.1076
Nora Mady, Nada M. Mostafa, Maha El-Batsh, Abdelfattah Saoud, A. Singab
Eggshell membrane (ESM), a thin membrane lining the eggshell of chicken eggs, is a natural byproduct attracting the attention of many researchers worldwide due to its valuable composition and beneficial biological activities. Its main active ingredients include collagen (types I, V, X), glucosamine sulfate, chondroitin/dermatan sulfate, and hyaluronic acid, so it possesses a beneficial value for maintaining healthy joints. Variation in eggshell color/breed of hens has been reported to affect the quality and composition of eggs and eggshells. So, the current study aimed to compare glucosamine sulfate content in ESM separated from white and brown eggs using the HPLC technique and UV detection at 195 nm. The peak area quantification method was used to determine the concentration of glucosamine in both samples. Results revealed that ESM separated from white eggs has a higher content of glucosamine sulfate (11.83% w/w) than ESM separated from brown eggs (10.68% w/w). We conclude that the use of ESM from white eggs may be more valuable than ESM from brown eggs in further biological studies to determine the effect of ESM in the prevention and management of osteoarthritis.
{"title":"HPLC Quantification of Glucosamine Sulfate in Eggshell membranes from White and Brown Chicken Eggs","authors":"Nora Mady, Nada M. Mostafa, Maha El-Batsh, Abdelfattah Saoud, A. Singab","doi":"10.21608/aps.2022.106854.1076","DOIUrl":"https://doi.org/10.21608/aps.2022.106854.1076","url":null,"abstract":"Eggshell membrane (ESM), a thin membrane lining the eggshell of chicken eggs, is a natural byproduct attracting the attention of many researchers worldwide due to its valuable composition and beneficial biological activities. Its main active ingredients include collagen (types I, V, X), glucosamine sulfate, chondroitin/dermatan sulfate, and hyaluronic acid, so it possesses a beneficial value for maintaining healthy joints. Variation in eggshell color/breed of hens has been reported to affect the quality and composition of eggs and eggshells. So, the current study aimed to compare glucosamine sulfate content in ESM separated from white and brown eggs using the HPLC technique and UV detection at 195 nm. The peak area quantification method was used to determine the concentration of glucosamine in both samples. Results revealed that ESM separated from white eggs has a higher content of glucosamine sulfate (11.83% w/w) than ESM separated from brown eggs (10.68% w/w). We conclude that the use of ESM from white eggs may be more valuable than ESM from brown eggs in further biological studies to determine the effect of ESM in the prevention and management of osteoarthritis.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84132880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/aps.2021.87917.1066
H. Ungo-kore, J. Ehinmidu, J. Onaolapo, O. Olonitola
Essential oil (EO) may confer a possible panacea to fight against multidrug-resistant dermatophytes. This study aims at determining the antidermatophytic activity of EO from the fruit peel of Nigerian Citrus senensis (L.) Osbeck against multidrug-resistant (MDR) tinea capitis dermatophytes and their possible chemical composition. The EO was reaped by hydro-distillation and the chemical composition was investigated using gas chromatography/ mass spectroscopy (GC-MS). The antidermatophytic activity was evaluated using the agar diffusion method against four dermatophyte strains (Two MDR dermatophytes, one reference strain, and one susceptible). The minimum inhibitory and fungicidal concentrations (MIC/MFC) were determined by agar dilution methods. The test results revealed EO to be active against all strains. The EO subjected to boiling temperature (100 0 C) had an increased amount of α-terpineol, β-Linalool, Carveol, Carvone, and other imaginative compounds such as 1-Octanol (Heptyl carbinols) with increased antidermatophytic activity. Our study suggests that this EO could be used clinically to treat or prevent dermatophytic infections associated with multidrug-resistant strains.
{"title":"Assessment of Antidermatophytic Activity and chemical composition of Nigerian Citrus senensis (L.) Osbeck Essential Oil against Multidrug-Resistant Pathogenic dermatophytes Isolated from Tinea capitis Samples","authors":"H. Ungo-kore, J. Ehinmidu, J. Onaolapo, O. Olonitola","doi":"10.21608/aps.2021.87917.1066","DOIUrl":"https://doi.org/10.21608/aps.2021.87917.1066","url":null,"abstract":"Essential oil (EO) may confer a possible panacea to fight against multidrug-resistant dermatophytes. This study aims at determining the antidermatophytic activity of EO from the fruit peel of Nigerian Citrus senensis (L.) Osbeck against multidrug-resistant (MDR) tinea capitis dermatophytes and their possible chemical composition. The EO was reaped by hydro-distillation and the chemical composition was investigated using gas chromatography/ mass spectroscopy (GC-MS). The antidermatophytic activity was evaluated using the agar diffusion method against four dermatophyte strains (Two MDR dermatophytes, one reference strain, and one susceptible). The minimum inhibitory and fungicidal concentrations (MIC/MFC) were determined by agar dilution methods. The test results revealed EO to be active against all strains. The EO subjected to boiling temperature (100 0 C) had an increased amount of α-terpineol, β-Linalool, Carveol, Carvone, and other imaginative compounds such as 1-Octanol (Heptyl carbinols) with increased antidermatophytic activity. Our study suggests that this EO could be used clinically to treat or prevent dermatophytic infections associated with multidrug-resistant strains.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83728625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/aps.2021.108852.1074
Amany Elgarf, Maram M. M. Aboromia, N. Sabri, S. Shaheen
Endothelial dysfunction is a crucial contributor to the development of vascular problems. Nigella sativa L. seed oil which is commonly known as black seed oil is an antioxidant, anti-inflammatory, and hypoglycemic agent that can be considered as an attractive candidate for improving endothelial dysfunction. Our objective is to evaluate the impact of Nigella sativa seed oil on intercellular adhesion molecule-1 (ICAM-1) and high sensitivity c-reactive protein (hsCRP), as well as the quality of life of type 2 diabetic patients. A prospective, randomized, placebocontrolled, double-blinded study was conducted and registered in ClinicalTrials.gov (NCT03959306). Patients were randomly given either 1800 mg/day of black seed oil or placebo capsules for 12 weeks. Full clinical history, biochemical glycemic indices, lipid profile, kidney and liver functions, hsCRP, ICAM-1, as well as diabetes-39 questionnaire were assessed at baseline and end of the study. After 12 weeks, the levels of hemoglobin A1C, total cholesterol, triglycerides, hsCRP, and ICAM-1, as well as diabetes control domain scores decreased significantly in the intervention group compared to the control group. In conclusion, the administration of black seed oil over 12 weeks showed superior efficacy over standard treatment alone in the management of type 2 diabetes mellitus and can be considered as a therapeutic option for improving endothelial dysfunction.
{"title":"Amelioration of Endothelial Dysfunction and Inflammation in Type 2 Diabetic Patients after Black Seed Oil Supplementation.","authors":"Amany Elgarf, Maram M. M. Aboromia, N. Sabri, S. Shaheen","doi":"10.21608/aps.2021.108852.1074","DOIUrl":"https://doi.org/10.21608/aps.2021.108852.1074","url":null,"abstract":"Endothelial dysfunction is a crucial contributor to the development of vascular problems. Nigella sativa L. seed oil which is commonly known as black seed oil is an antioxidant, anti-inflammatory, and hypoglycemic agent that can be considered as an attractive candidate for improving endothelial dysfunction. Our objective is to evaluate the impact of Nigella sativa seed oil on intercellular adhesion molecule-1 (ICAM-1) and high sensitivity c-reactive protein (hsCRP), as well as the quality of life of type 2 diabetic patients. A prospective, randomized, placebocontrolled, double-blinded study was conducted and registered in ClinicalTrials.gov (NCT03959306). Patients were randomly given either 1800 mg/day of black seed oil or placebo capsules for 12 weeks. Full clinical history, biochemical glycemic indices, lipid profile, kidney and liver functions, hsCRP, ICAM-1, as well as diabetes-39 questionnaire were assessed at baseline and end of the study. After 12 weeks, the levels of hemoglobin A1C, total cholesterol, triglycerides, hsCRP, and ICAM-1, as well as diabetes control domain scores decreased significantly in the intervention group compared to the control group. In conclusion, the administration of black seed oil over 12 weeks showed superior efficacy over standard treatment alone in the management of type 2 diabetes mellitus and can be considered as a therapeutic option for improving endothelial dysfunction.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73074842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/aps.2021.92280.1067
Mery Sameh Waheeb, W. Elkhatib, M. Yassien, Nadia A. Hassouna
Cellulases have been considered effective biocatalysts because of their diversity of applications. A total of 165 cellulase-producing Streptomyces isolates were recovered from Egyptian soil samples via cultivation on carboxymethyl cellulose agar medium. The results of screening by the Congo red method showed that the tested isolates revealed different levels of cellulase activity. The isolates showed a high level of cellulase production (clear zones ˃ 25 mm, n= 15) were submitted for quantitative evaluation of their cellulase productivities by using dinitrosalicylic acid assay. Cellulase production of these selected isolates ranged from 146.9 to 650.5 U/L. The highest cellulase-producing isolate (S11-6) was identified at the molecular level using 16S rRNA gene sequencing. The sequence homology analysis proved its proximity and relatedness to Streptomyces coelicoflavus strain NBRC 15399. Production improvement of cellulase was carried out by genetic manipulation using a dose of 4 Kilogray (KGy) of gamma radiation. M1 mutant showed 1.31 fold increments in cellulase production as compared to the wild-type strain of S. coelicoflavus.
纤维素酶因其应用的多样性而被认为是有效的生物催化剂。通过在羧甲基纤维素琼脂培养基上培养，从埃及土壤样品中回收了165株产纤维素酶链霉菌。刚果红法筛选结果表明，各分离株纤维素酶活性不同。采用二硝基水杨酸法对分离株纤维素酶产率进行定量评价，结果表明分离株纤维素酶产率较高(透明区≤25 mm, n= 15)。所选菌株的纤维素酶产量为146.9 ~ 650.5 U/L。通过16S rRNA基因测序，在分子水平上鉴定出产纤维素酶最高的分离物(S11-6)。序列同源性分析证实该菌株与coelicoflavus链霉菌NBRC 15399具有亲缘关系。利用4千格(KGy)的伽马辐射进行基因操作，提高纤维素酶的产量。与野生型菌株相比，M1突变体纤维素酶产量增加了1.31倍。
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