Aquaculture最新文献

{"title":"The 2023 National Proficiency Test for molecular detection of shrimp infectious myonecrosis virus (IMNV) in China","authors":"Chen Li ,&nbsp;Bing Yang ,&nbsp;Meifeng Wang ,&nbsp;Xiaoyuan Wan ,&nbsp;Weizhong Yu ,&nbsp;Wenqiang Li ,&nbsp;Xiaoling Song ,&nbsp;Li Liu ,&nbsp;Qing Li ,&nbsp;Qingli Zhang","doi":"10.1016/j.aquaculture.2024.741775","DOIUrl":"10.1016/j.aquaculture.2024.741775","url":null,"abstract":"<div><div>Infectious myonecrosis virus (IMNV), an emerging virus that can cause severe viral disease in penaeid shrimp, was first confirmed to have spread to China in 2020. In order to standardize diagnostic procedures and reliable detection capability, and to evaluate the performances of aquatic clinical diagnostic laboratories, the executive agency has launched a proficiency test (PT) scheme for the molecular detection of IMNV in China from the year of 2023. In the ring test of IMNV-PT 2023, five tissue samples containing different concentrations of IMNV were provided as a panel of test samples. A total of 68 laboratories participated in the PT test of qualitative and quantitative analysis of the five samples using standard molecular assays. The results showed that 92.65 % (63/68) of the participants correctly reported all samples in the panel using Real-time reverse transcription PCR and 62 participants achieved satisfactory results, one participant using conventional PCR and four participants had not completed the test. Out of the 62 participants in the <em>z</em>-scores analysis, 88.71 % (55/62) achieved acceptable <em>ZB</em>-scores, with two outlier laboratories and five laboratories being questionable. Additionally, 98.39 % (61/62) achieved <em>ZW</em>-scores, with only one laboratory being questionable. Furthermore, the national PT conducted in the present study proved to be an effective tool for evaluating laboratory performance in IMNV molecular testing. This assessment can aid in improving the capabilities of IMNV molecular testing of the participating laboratories, and also provide technical support to enhance the national response to IMN outbreaks.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741775"},"PeriodicalIF":3.9,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142529560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The size of the sperm head influences the gynogenetic success in teleost fish","authors":"Moe Takeuchi ,&nbsp;Yoshifumi Kawamura ,&nbsp;Tomomitsu Arai ,&nbsp;Shigeho Ijiri ,&nbsp;Eisuke Takahashi ,&nbsp;Etsuro Yamaha ,&nbsp;Takafumi Fujimoto ,&nbsp;Toshiya Nishimura","doi":"10.1016/j.aquaculture.2024.741768","DOIUrl":"10.1016/j.aquaculture.2024.741768","url":null,"abstract":"<div><div>Gynogenesis is a phenomenon in which only the maternal genome contributes to offspring development. Artificial gynogenesis induced using genetically inactivated sperm is a useful technique for sex control and rapid production of inbred lines in aquaculture and breeding. For gynogenesis induction, eggs require fertilization stimulus from the sperm; however, the effects of sperm characteristics on gynogenetic success remain unexplored. In this study, we investigated the induction of gynogenesis in medaka embryos by sperm from distant species and the correlation of sperm head size relative to the micropyle with success in gynogenesis. Results revealed that sperm heads of Nile tilapia and rainbow trout had smaller areas than those of the medaka micropyle, and thus, the sperm successfully activated medaka eggs for development. Furthermore, gynogenetic haploid embryos could be induced using genetically inactivated sperm from these species, which were viable after diploidization using heat shock treatment. In contrast, goldfish and zebrafish sperm, with larger head areas than that of medaka micropyle, could not activate medaka eggs for development, suggesting that sperm entry into medaka micropyle was inhibited. Therefore, we propose that the relative physical features of sperm and eggs influence the success of gynogenesis. The findings provide new insights into the application of gynogenesis in aquacultural fish. We developed an automatic method for measuring sperm head size, which can facilitate the recording of physical characteristics of sperm and predicting gynogenesis success.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741768"},"PeriodicalIF":3.9,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin supplementation improved the growth and health of juvenile Chinese mitten crabs (Eriocheir sinensis) fed low-fishmeal diets","authors":"Yingying Lin ,&nbsp;Qiuran Yu ,&nbsp;Zhijun Liu ,&nbsp;Xuesong Li ,&nbsp;Xiaodan Wang ,&nbsp;Jian G. Qin ,&nbsp;Liqiao Chen ,&nbsp;Erchao Li","doi":"10.1016/j.aquaculture.2024.741773","DOIUrl":"10.1016/j.aquaculture.2024.741773","url":null,"abstract":"<div><div>The substitution of fishmeal with plant proteins can induce oxidative stress in aquatic animals and impair their growth and health. Quercetin is a potent natural antioxidant, but its specific effects on the growth and health of Chinese mitten crabs, as well as the underlying molecular mechanisms, remain unclear. This study aimed to investigate the impact of quercetin on the growth, physiological and biochemical parameters, gut microbiota, and transcriptome of juvenile Chinese mitten crabs (<em>Eriocheir sinensis</em>). Crabs (0.53 ± 0.01 g) were randomly divided into seven groups. One group was fed a diet containing normal fishmeal (35 % fishmeal), whereas the other six groups were fed diets containing low levels of fishmeal (15 % fishmeal) supplemented with different levels of quercetin (0, 250, 500, 1000, 2000, or 4000 mg/kg). Each group had four replicate tanks with 40 crabs per 300 L tank, and the experiment lasted for 56 days. The growth performance of the 35 % fishmeal group was significantly greater than that of the 15 % fishmeal group without quercetin. However, quercetin significantly increased the weight gain rate and specific growth rate of juvenile crabs, especially in the 1000 mg/kg quercetin group, where these rates exceeded those of the normal fishmeal group. The antioxidant capacity and immunity of the 35 % fishmeal group were significantly greater than those of the 15 % fishmeal group. However, quercetin supplementation promoted the antioxidant capacity and immunity of the crabs. Quercetin supplementation at 4000 mg/kg changed the gut microbiota structure by decreasing beneficial bacteria and increasing pathogenic bacteria. Quercetin supplementation enhanced the growth performance and antioxidant capacity of Chinese mitten crabs by increasing glutamate metabolism, pantothenic acid and Coenzyme A biosynthesis, sphingolipid metabolism, arachidonic acid metabolism, and cysteine and methionine metabolism. This study revealedthat dietary supplementation with quercetin can promote Chinese mitten crab growth, antioxidant capacity, and nonspecific immunity; improve gut morphology; and promote gut microbiota homeostasis. The optimal dietary concentration of 688–695 mg/kg is recommended on the basis of weight gain and specific growth rate analyses.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741773"},"PeriodicalIF":3.9,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142529297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of stocking density of largemouth bass on the self-cleaning performance of a circular aquaculture tank","authors":"Xiaoyu Qu ,&nbsp;Yi Tao ,&nbsp;Dezhen Li ,&nbsp;Fuzhi Zu ,&nbsp;Yanfei Wu ,&nbsp;Fei Wang ,&nbsp;Lianhui Wu ,&nbsp;Jiajun Hu ,&nbsp;Yang Gao ,&nbsp;Dejun Feng","doi":"10.1016/j.aquaculture.2024.741770","DOIUrl":"10.1016/j.aquaculture.2024.741770","url":null,"abstract":"<div><div>Reasonable regulation of stocking density enables aquaculture tanks to achieve excellent self-cleaning performance, which plays a crucial role in the healthy growth and overall welfare of fish. Enhancing the waste collection, and removal capacity and improving the hydrodynamic characteristics in these tanks are pressing issues in the development of recirculating aquaculture systems (RAS) toward sustainable aquaculture. This study investigates the impact of fish (largemouth bass) stocking density (3, 6, and 9 kg/m³) on the self-cleaning performance of circular aquaculture tanks under single- and dual-inlet modes through experiments. The effects of stocking density and fish behavior on the hydrodynamic characteristics of the tanks were analyzed using the average velocity <em>v</em>_<em>avg</em>, drag coefficient <em>C</em>_<em>t</em>, and turbulence intensity root mean square (RMS). Finally, the sensitivity analysis method was adopted to quantify the sensitivity (<em>S</em>) of RMS to changes in stocking density and inflow rate under two different water-inlet modes. This allows the comparison of the comprehensive impacts of stocking density and inflow rate on the self-cleaning performance of culture tanks. The results indicate that under a single-inlet mode, adjusting <em>Q</em> = 8 L/min and <em>ρ</em> = 9 kg/m³ maximizes the self-cleaning performance of the tank compared with fishless conditions, with waste collection efficiency increasing by approximately 4.3 times, under a dual-inlet mode, adjusting <em>Q</em> = 10 L/min and <em>ρ</em> = 9 kg/m³ results in the greatest enhancement in self-cleaning performance relative to fishless conditions, with an increase in waste collection efficiency of approximately 3.44 times. In both single- and dual-inlet modes, when the stocking density increases from 0 to 9 kg/m³, the RMS values increase by a maximum of approximately 2.3 and 1.2 times, respectively. Furthermore, when 6 kg/m³ &lt; <em>ρ &lt;</em> 9 kg/m³ and 8 L/min &lt; <em>Q &lt;</em> 10 L/min, the RMS is more sensitive to changes in stocking density under a single-inlet mode and is more sensitive to changes in inflow rate under a dual-inlet mode. The findings can offer guidance on the optimal stocking densities for aquaculture tanks to obtain superior self-cleaning performance, and contribute to the practical management of RAS.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741770"},"PeriodicalIF":3.9,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142529299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of the molting cycle on the acoustic characteristics of clicks emitted by Litopenaeus vannamei","authors":"Silvio Peixoto,&nbsp;Cecilia Craveiro,&nbsp;Fábio Costa Filho,&nbsp;Hildemário Castro-Neto,&nbsp;Roberta Soares","doi":"10.1016/j.aquaculture.2024.741761","DOIUrl":"10.1016/j.aquaculture.2024.741761","url":null,"abstract":"<div><div>In penaeid shrimp, the mandibles are responsible for emitting click sounds when they collide during food intake, which has been used to assess feeding activity by passive acoustic monitoring. However, the acoustic parameters of the clicks could be affected by variations in the thickness of mandible cuticle during the molting cycle. Thus, the aim of this study was to evaluate the effects of the molting cycle on the acoustic characteristics of clicks emitted during the feeding activity of <em>Litopenaeus vannamei</em> fed commercial pelleted diet. Shrimp with an average weight of 8.75 ± 1.05 g were individually maintained in 24 tanks in a recirculating water system. A total of 12 animals had their feeding activity individually recorded soon after ecdysis (postmolt group), while another 12 were recorded in the middle of their molting cycle (intermolt group). Recordings took place in anechoic chambers with a hydrophone connected to a digital recorder (sampling rate of 192 kHz), and the click acoustic parameters were characterized in Raven® 1.5 Pro software. After the recordings, the shrimp had their mandibles removed for length measurement and histological analysis of the mandibular cuticle. Body (weight and length) and mandibular measurements did not differ between shrimp groups, but those in the postmolt stage showed significantly lower cuticle thickness. The acoustic characteristics of the clicks were affected by shrimp molting stages, with significantly lower maximum energy (dB) and maximum frequency (kHz) in postmolt shrimp, probably related to their lower mandibular cuticle thickness. These results may contribute to the assessment of shrimp feeding behavior associated with the molting cycle, as well as to the optimization of algorithms controlling acoustic automatic feeders in farming systems.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741761"},"PeriodicalIF":3.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable DNA extraction and loop-mediated isothermal amplification (LAMP) for the on-site detection of white spot syndrome virus (WSSV)","authors":"T. Sathish Kumar,&nbsp;S. Suvetha,&nbsp;B. Naveen Rajeshwar,&nbsp;M. Makesh,&nbsp;M. Shashi Shekhar,&nbsp;Kuldeep K. Lal","doi":"10.1016/j.aquaculture.2024.741760","DOIUrl":"10.1016/j.aquaculture.2024.741760","url":null,"abstract":"<div><div>White spot disease (WSD) has been the most severe and lethal threat to the global shrimp industry for the past three decades. WSD caused by white spot syndrome virus (WSSV) can result in severe economic losses in shrimp culture. The disease can cause rapid and mass mortality of 90–100 % within 3–10 days. Currently, there are no effective treatment measures available for the control of WSD. Strict biosecurity, possible elimination, and early diagnosis can prevent the spread of the pathogen. Several advanced diagnostic methods have been standardised for the early and rapid detection of WSD. However, the field deployable diagnostic methods are limited. LAMP is a cost-effective, alternative nucleic acid amplification method that operates under isothermal conditions. In this study, a novel field-portable LAMP was developed for the diagnosis of WSSV. The WSSV amplification was optimized at an isothermal condition of 65 °C and a reaction time of 55 min. In this closed-tube amplification, the end results were easily visualized with the naked eye by observing the simple color change. This assay is highly sensitive and can detect up to 6 copies of WSSV plasmid DNA and 0.1 fg of WSSV genomic DNA. Additionally, this diagnostic method is highly specific and did not cross-react with other shrimp pathogens such as IHHNV, IMNV, EHP and <em>Vibrio parahaemolyticus</em> or host DNA of <em>Penaeus vannamei, P. monodon, and P. indicus</em>. Furthermore, a field-deployable DNA (FDD) extraction method has been standardised with a simple rotor and dry bath. This visual LAMP-coupled FDD extraction did not require any sophisticated equipment or technical expertise. Thus this robust LAMP assay can potentially be used for routine surveillance, point-of-care testing, early detection, and timely intervention of WSD.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741760"},"PeriodicalIF":3.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142529557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salt tolerance candidate genes identified by QTL mapping, RNA-seq, and functional analysis in tilapia","authors":"Zituo Yang ,&nbsp;Le Wang ,&nbsp;Fei Sun ,&nbsp;Joey Wong ,&nbsp;May Lee ,&nbsp;Shadame Yeo ,&nbsp;Yanfei Wen ,&nbsp;Gen Hua Yue","doi":"10.1016/j.aquaculture.2024.741762","DOIUrl":"10.1016/j.aquaculture.2024.741762","url":null,"abstract":"<div><div>Salt tolerance in fish is crucial for aquaculture as it enhances survival and productivity in varying salinity conditions, thus expanding the range of viable farming environments and improving economic sustainability. Through QTL mapping and GWAS in a hybrid F₂ family of Mozambique and Nile tilapia, two large-effect QTL on chromosomes 11 and 18 were identified respectively. These two QTL explained a total of 39.9 % of the phenotypic variance. The identification of a QTL on LG11 suggests the presence of a previously unrecognized genetic factor contributing to salt tolerance. Comparative transcriptomic analysis of gill and kidney tissues between susceptible and tolerant tilapias highlights the importance of osmotic balance in regulation of salt tolerance in tilapia. Integration of QTL and RNA-seq data identified two candidate genes: acyl-coenzyme A thioesterase 5 (<em>acot5</em>) and sodium- and chloride-dependent taurine transporter (<em>slc6a6</em>) likely playing critical roles in such process. Functional analysis showed that over-expressing <em>acot5</em> or <em>slc6a6</em> in grouper kidney cells increased viability under salt stress by 4.46 % and 17.53 %, respectively. Subcellular localization revealed nuclear presence of ACOT5 and stress-induced nuclear translocation of SLC6A6. These findings highlight <em>acot5</em> and <em>slc6a6</em> as candidates for genetic manipulation and selection to enhance salt tolerance in tilapia, guiding genetic improvement efforts and promoting sustainable practices.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741762"},"PeriodicalIF":3.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ca2+-SpVAMP2 pathway promotes exosome secretion to resist the infection of Vibrio parahaemolyticus in mud crab (Scylla paramamosain)","authors":"Yifan Lei ,&nbsp;Ming Zhang ,&nbsp;Kangyue Huang ,&nbsp;Qian Sun ,&nbsp;Jinkun Li ,&nbsp;Huifen Liang ,&nbsp;Yuqing Zheng ,&nbsp;Ngoc Tuan Tran ,&nbsp;Xiuli Chen ,&nbsp;Yanqiu Zhang ,&nbsp;Yueling Zhang ,&nbsp;Shengkang Li","doi":"10.1016/j.aquaculture.2024.741766","DOIUrl":"10.1016/j.aquaculture.2024.741766","url":null,"abstract":"<div><div>Exosomes play an important role in the innate immune system. Many types of cells can secrete exosomes in both normal and abnormal status. However, the mechanism of exosome secretion in invertebrates has not been reported. In this study, the mechanism of the secretion of exosomes regulated by the Ca²⁺ signaling pathway in the hemocytes of mud crabs (<em>Scylla paramamosain</em>) under infection with <em>Vibrio parahaemolyticus</em> was investigated. The results showed that the increase of exosome secretion in mud crabs infected with <em>V. parahaemolyticus</em> was caused by the increase in intracellular Ca²⁺ level. In addition, transcriptome sequencing and differential expression analysis revealed that <em>Sp</em>VAMP2, a vesicle-associated membrane protein 2, was found to be involved in the exosome secretion regulated by Ca²⁺ signaling. Further research revealed that <em>Sp</em>VAMP2 can inhibit MVB from entering the lysosome for degradation and promote the release of exosomes. Additionally, the secretion of exosomes upregulated the expression of anti-lipopolysaccharide factors and enhanced the survival rate of mud crabs after <em>V. parahaemolyticus</em> infection. This study clarified the exosome secretion regulated by the Ca²⁺-<em>Sp</em>VAMP2 pathway, which, in turn, increased the innate immune response in mud crabs to resist the invasion of <em>V. parahaemolyticus</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741766"},"PeriodicalIF":3.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142529558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the pharmaceutical footprint and risks of combining different aquaculture modes and tailwater treatment systems","authors":"Feng Xu ,&nbsp;Yangcai Wang ,&nbsp;Qiangqiang Shi ,&nbsp;Yinliang Wu ,&nbsp;Zeming Zhang ,&nbsp;Xizhi Shi","doi":"10.1016/j.aquaculture.2024.741763","DOIUrl":"10.1016/j.aquaculture.2024.741763","url":null,"abstract":"<div><div>Aquatic environments serve as repositories for pharmaceuticals, and intensive aquaculture is becoming a significant contributor to the discharge of aquaculture wastewater. The cyclical footprint of pharmaceuticals between the aquatic environment and aquaculture has emerged as a global concern. The environmental occurrence and risk assessment of pharmaceuticals in the aquatic environment via various aquaculture modes, as well as the pharmaceutical removal efficiency in different tailwater treatment systems, have been studied through yearly investigations. Thirty-four pharmaceuticals were detected in the aquaculture environment, and the dominant pharmaceuticals were enrofloxacin, sulfamethoxazole, amantadine, and florfenicol. The highest concentration of pharmaceuticals was found in the fish pond (3826 ± 29.6 ng/L) under the greenhouse aquaculture mode, especially in the early and late aquaculture stages, and the same trend was observed in the pond aquaculture mode. The tailwater removal efficiency was greater in summer months than other months, and the treatment efficiency of the recirculating treatment system was more stable. During the aquaculture period, a low risk was observed for most pharmaceuticals, except for enrofloxacin, sulfamethoxazole, ciprofloxacin and ofloxacin, which had at medium to high risk. High-risk pharmaceuticals show a high potential to contaminate the surrounding aquatic environment and adversely affect associated biota. Reassuringly, the ecological risks of the detected pharmaceuticals can be effectively reduced after tailwater treatment. This study provides valuable data for the growing system to assess risk in aquaculture and serves as a reference to establish quality standards for aquatic products.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741763"},"PeriodicalIF":3.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-method approach to assessing consumer acceptance of sustainable aquaponics","authors":"Jeffrey Meyer ,&nbsp;Fei L. Weisstein ,&nbsp;Jonathan Kershaw ,&nbsp;Kevin Neves","doi":"10.1016/j.aquaculture.2024.741764","DOIUrl":"10.1016/j.aquaculture.2024.741764","url":null,"abstract":"<div><div>Aquaponics is a sustainable production method of co-cultivating fish and vegetables in a water-recirculating system that resembles a natural ecosystem. For aquaculturists to embrace aquaponics technology, understanding marketing positioning for both aquaponic fish and vegetables is crucial. While prior research suggests highlighting aquaponic fish's environmental benefits may improve consumer perceptions, marketing strategies for aquaponic produce remain underexplored. This research addresses consumers' concerns and identifies key communication elements that enhance consumers' sensory liking and willingness-to-pay (WTP) for aquaponic produce. Employing a multi-method approach, we conducted a taste test of 191 consumers (Study 1) to assess consumers' actual tasting experience of aquaponic produce and a choice-based conjoint analysis of 986 consumers (Study 2) to quantify their WTP. The results show that innovative aquaponics has no adverse effect on consumers' sensory liking when properly defined with a sustainability focus. Strategically aligning aquaponics benefits message focus (eco-friendly versus clean-and-safe) with consumer motivational factors (environmental concern versus health consciousness) increases sensory liking and WTP. Environmental-conscious consumers have a greater sensory liking for aquaponic produce promoted as eco-friendly but not clean-and-safe. Environmental- and health-conscious consumers have a higher WTP for eco-friendly focused aquaponic than field-grown produce. Health-conscious consumers have a higher WTP for clean-and-safe focused aquaponics. While consumers generally value local and small family farm aquaponics attributes over an organic attribute, the effect varied by their motivational factors. These findings provide insights for aquaculturists to strategically position aquaponics, supporting the transition towards more sustainable food production and consumption amid growing global food demands.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741764"},"PeriodicalIF":3.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}