Pub Date : 2024-11-02DOI: 10.1016/j.aquaculture.2024.741841
A.M. Karami , P.W. Kania , A. Al-Jubury , D. Stefanova , L. Krych , L. Madsen , T. Nielsen , K. Buchmann
Two genetic lineages of rainbow trout Oncorhynchus mykiss, with relatively high (HR) and low (LR) resistance towards the bacterial pathogen Flavobacterium psychrophilum, respectively, were produced based on Chr25 QTL marker assisted selection. A controlled bath challenge with F. psychrophilum in a common garden set-up confirmed the differential resistance status of the fish strains. Analysis of surviving fish thirty days post-challenge showed that the expression of immune related genes in the intestinal wall of the fish differed significantly between high resistant HR and LR fish. A range of genes encoding cytokines (IL-1, Il-2, IL-4/13, IL-6, IL-10, IL-17, IL-22), innate immune effector molecules (SAA, cathelicidins, complement, hepcidin) and the immunoglobulin IgD gene were significantly upregulated in the HR fish, whereas corresponding genes (except of the SAA gene) remained unregulated in LR fish. We analyzed the gut content from fish in all groups in order to elucidate to what extent the gut microbiota could be influenced by host genes and pathogen exposure. The alpha diversity was significantly higher in HR compared to LR before infection, but the difference was not significant after the bacterial exposure. The beta diversity showed significant differences between two genetic groups which faded after exposure. The study suggests a continuous cross-talk between host genetics, innate immune responses in the host intestinal wall and the microbial community of the fish gut.
在 Chr25 QTL 标记辅助选择的基础上，产生了虹鳟鱼的两个基因系，它们对细菌病原体 Flavobacterium psychrophilum 的抗性分别相对较高（HR）和较低（LR）。在一个普通的园林环境中，用F. psychrophilum进行控制性水浴挑战，证实了鱼品系的不同抗性状态。对挑战后 30 天存活鱼类的分析表明，高抗性 HR 和 LR 鱼类肠壁中免疫相关基因的表达有显著差异。编码细胞因子（IL-1、Il-2、IL-4/13、IL-6、IL-10、IL-17、IL-22）、先天性免疫效应分子（SAA、柔毛素、补体、肝素）和免疫球蛋白 IgD 基因的一系列基因在高抗性鱼类中显著上调，而在低抗性鱼类中，相应基因（除 SAA 基因外）仍未上调。我们分析了各组鱼的肠道内容物，以阐明肠道微生物群在多大程度上受宿主基因和病原体暴露的影响。感染前，HR 的α多样性明显高于 LR，但细菌暴露后差异不明显。贝塔多样性在两个基因组之间存在明显差异，但在接触细菌后差异逐渐消失。这项研究表明，宿主遗传学、宿主肠壁的先天免疫反应和鱼类肠道微生物群落之间存在持续的交叉对话。
{"title":"Gut microbiota in rainbow trout Oncorhynchus mykiss with different susceptibility to Flavobacterium psychrophilum infection","authors":"A.M. Karami , P.W. Kania , A. Al-Jubury , D. Stefanova , L. Krych , L. Madsen , T. Nielsen , K. Buchmann","doi":"10.1016/j.aquaculture.2024.741841","DOIUrl":"10.1016/j.aquaculture.2024.741841","url":null,"abstract":"<div><div>Two genetic lineages of rainbow trout <em>Oncorhynchus mykiss</em>, with relatively high (HR) and low (LR) resistance towards the bacterial pathogen <em>Flavobacterium psychrophilum</em>, respectively, were produced based on Chr25 QTL marker assisted selection. A controlled bath challenge with <em>F. psychrophilum</em> in a common garden set-up confirmed the differential resistance status of the fish strains. Analysis of surviving fish thirty days post-challenge showed that the expression of immune related genes in the intestinal wall of the fish differed significantly between high resistant HR and LR fish. A range of genes encoding cytokines (IL-1, Il-2, IL-4/13, IL-6, IL-10, IL-17, IL-22), innate immune effector molecules (SAA, cathelicidins, complement, hepcidin) and the immunoglobulin IgD gene were significantly upregulated in the HR fish, whereas corresponding genes (except of the SAA gene) remained unregulated in LR fish. We analyzed the gut content from fish in all groups in order to elucidate to what extent the gut microbiota could be influenced by host genes and pathogen exposure. The alpha diversity was significantly higher in HR compared to LR before infection, but the difference was not significant after the bacterial exposure. The beta diversity showed significant differences between two genetic groups which faded after exposure. The study suggests a continuous cross-talk between host genetics, innate immune responses in the host intestinal wall and the microbial community of the fish gut.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741841"},"PeriodicalIF":3.9,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.aquaculture.2024.741848
Jeong-Eun Lee , Hee-Kyeong Yang , Kwang-Soo Ha , Young-Mog Kim , Ji Yoon Chang , Won-Bo Shim
Kudoa septempunctata, a myxosporean parasite newly identified in 2010, was first detected in the trunk muscle of an aquacultured olive flounder imported from Korea to Japan. Since 2003, foodborne disease outbreaks in Japan have been increasing, often linked to the ingestion of raw olive flounder. These illnesses, characterized by diarrhea and emesis, typically occur within 2–20 h after consuming raw olive flounder. Recent studies on the etiological agents responsible for these illnesses and animal experiments have implicated K. septempunctata as a potential causative agent of these novel foodborne disease outbreaks. Consequently, a rapid, sensitive, and convenient method for detecting K. septempunctata is required. This study presents a colorimetric loop-mediated isothermal amplification (LAMP) assay developed using a molecular beacon (MB) and horseradish peroxidase-mimicking DNAzyme for the rapid and sensitive detection of K. septempunctata in the muscle of olive flounder. Three pairs of primers targeting the 28S rDNA gene and an MB were designed and synthesized. The colorimetric LAMP assay was optimized by determining key factors such as MB concentration, incubation temperature, and time. Its specificity was investigated, and the method was validated using contaminated muscle samples of olive flounder (101–106 spores/g). The cutoff value of the colorimetric LAMP assay, optimized at 57.5 °C, was 1 × 101 spores/g, confirming its specificity to K. septempunctata. The developed assay can be completed within 1 h. Overall, this study demonstrates that the developed K. septempunctata-specific LAMP assay shows promise as a point-of-care molecular diagnostic technology, as it does not require expensive instruments such as a thermocycler or detector.
{"title":"Point-of-care testing based on colorimetric loop-mediated isothermal amplification coupled with a nondestructive device for rapid detection of Kudoa septempunctata in live olive flounder","authors":"Jeong-Eun Lee , Hee-Kyeong Yang , Kwang-Soo Ha , Young-Mog Kim , Ji Yoon Chang , Won-Bo Shim","doi":"10.1016/j.aquaculture.2024.741848","DOIUrl":"10.1016/j.aquaculture.2024.741848","url":null,"abstract":"<div><div><em>Kudoa septempunctata</em>, a myxosporean parasite newly identified in 2010, was first detected in the trunk muscle of an aquacultured olive flounder imported from Korea to Japan. Since 2003, foodborne disease outbreaks in Japan have been increasing, often linked to the ingestion of raw olive flounder. These illnesses, characterized by diarrhea and emesis, typically occur within 2–20 h after consuming raw olive flounder. Recent studies on the etiological agents responsible for these illnesses and animal experiments have implicated <em>K. septempunctata</em> as a potential causative agent of these novel foodborne disease outbreaks. Consequently, a rapid, sensitive, and convenient method for detecting <em>K. septempunctata</em> is required. This study presents a colorimetric loop-mediated isothermal amplification (LAMP) assay developed using a molecular beacon (MB) and horseradish peroxidase-mimicking DNAzyme for the rapid and sensitive detection of <em>K. septempunctata</em> in the muscle of olive flounder. Three pairs of primers targeting the <em>28S rDNA</em> gene and an MB were designed and synthesized. The colorimetric LAMP assay was optimized by determining key factors such as MB concentration, incubation temperature, and time. Its specificity was investigated, and the method was validated using contaminated muscle samples of olive flounder (10<sup>1</sup>–10<sup>6</sup> spores/g). The cutoff value of the colorimetric LAMP assay, optimized at 57.5 °C, was 1 × 10<sup>1</sup> spores/g, confirming its specificity to <em>K. septempunctata</em>. The developed assay can be completed within 1 h. Overall, this study demonstrates that the developed <em>K. septempunctata</em>-specific LAMP assay shows promise as a point-of-care molecular diagnostic technology, as it does not require expensive instruments such as a thermocycler or detector.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741848"},"PeriodicalIF":3.9,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.aquaculture.2024.741826
Rebecca L. Stobart , Andrew G. Jeffs , Jenny R. Hillman , Bradley M. Skelton
Fish predation poses a significant and escalating challenge for the global mussel aquaculture industry. In New Zealand, Greenshell™ mussel farms experience crop losses of up to 100 % that are anecdotally attributed to fish predation, although the fish species responsible have not been confirmed. In this study, remote underwater video (RUV) was used to observe fish activity within four mussel farms at different stages of production in the Firth of Thames, New Zealand. The RUV enabled the identification of the fish species inhabiting mussel farms, including those responsible for predating cultured mussels. Four fish species were identified as frequent inhabitants of the mussel farms, with Australasian snapper (Chrysophrys auratus) and parore (Girella tricuspidata) the most abundant at three out of four mussel farm sites. Australasian snapper was the most common predator, taking as many as 2880 bites of the dropper lines holding mussels in a single 11 min 47 s video recording. Snapper formed feeding aggregations around dropper lines holding juvenile mussels (> 20 mm SL), indicating their potential to remove mussels from nursery farms in a short amount of time. Observations of the feeding behaviour of parore indicated their potential to contribute to the losses of mussels on recently seeded spat farms with these fish recorded tearing apart seeded lines, possibly targeting the macroalgae that is seeded out with the mussel spat. Besides confirming the identity and the predatory behaviour of fish species that are contributing to crop losses from mussel farms in New Zealand, this study also confirms the effectiveness of RUV methods for this purpose. This knowledge can be used to begin to develop mitigation strategies aimed at reducing crop losses in mussel aquaculture.
{"title":"Assessing the use of remote underwater video (RUV) to identify fish and their feeding behaviour in New Zealand's Greenshell™ mussel (Perna canaliculus) farms","authors":"Rebecca L. Stobart , Andrew G. Jeffs , Jenny R. Hillman , Bradley M. Skelton","doi":"10.1016/j.aquaculture.2024.741826","DOIUrl":"10.1016/j.aquaculture.2024.741826","url":null,"abstract":"<div><div>Fish predation poses a significant and escalating challenge for the global mussel aquaculture industry. In New Zealand, Greenshell™ mussel farms experience crop losses of up to 100 % that are anecdotally attributed to fish predation, although the fish species responsible have not been confirmed. In this study, remote underwater video (RUV) was used to observe fish activity within four mussel farms at different stages of production in the Firth of Thames, New Zealand. The RUV enabled the identification of the fish species inhabiting mussel farms, including those responsible for predating cultured mussels. Four fish species were identified as frequent inhabitants of the mussel farms, with Australasian snapper (<em>Chrysophrys auratus</em>) and parore (<em>Girella tricuspidata</em>) the most abundant at three out of four mussel farm sites. Australasian snapper was the most common predator, taking as many as 2880 bites of the dropper lines holding mussels in a single 11 min 47 s video recording. Snapper formed feeding aggregations around dropper lines holding juvenile mussels (> 20 mm SL), indicating their potential to remove mussels from nursery farms in a short amount of time. Observations of the feeding behaviour of parore indicated their potential to contribute to the losses of mussels on recently seeded spat farms with these fish recorded tearing apart seeded lines, possibly targeting the macroalgae that is seeded out with the mussel spat. Besides confirming the identity and the predatory behaviour of fish species that are contributing to crop losses from mussel farms in New Zealand, this study also confirms the effectiveness of RUV methods for this purpose. This knowledge can be used to begin to develop mitigation strategies aimed at reducing crop losses in mussel aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741826"},"PeriodicalIF":3.9,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.aquaculture.2024.741844
Anran Wang , Xuran Liu , Jie Xu , Mengge Li , Dongxu Luo , Xingkong Ma , Yongtao Liu , Jiachun Ge , Qinghui Ai , Shuyan Miao
In this study, two animal experiments were designed to assess the function of arachidonic acid (ARA) on the ovarian development of Chinese mitten crab (Eriocheir sinensis). In experiment 1, female crabs (35.55 ± 0.02 g) were fed seven experimental diets, each containing varying levels of ARA (0.01 %, 0.28 %, 0.48 %, 0.67 %, 0.86 %, 1.02 %, and 1.21 %) for 120 d. Dietary 0.48 %–0.86 % ARA significantly improved the growth performance of crabs, and more than dietary 0.67 % ARA significantly increased the gonadosomatic index (GSI), ovarian vitellogenin (VTG) and vitellin (Vn) contents (P < 0.05). The egg diameter was largest when dietary 0.86 % ARA. Moreover, dietary ARA also significantly affected the hepatopancreatic and ovarian fatty acid compositions and ARA content by increasing the relative expressions of phospholipase A2 (pla2), cyclooxygenase-1(cox-1), cyclooxygenase-2 (cox-2) and 5-lipoxygenase (5-lox), while decreasing the relative expressions of fatty acid synthase (fas), fatty acid desaturase (fad6), lipoprotein lipase (lpl) and fatty acid elongase (elo) (P < 0.05). Based on the GSI and egg diameter, the amount of 0.86 % ARA was selected to further investigate the mechanisms involved in ovarian development. In experiment 2, female crabs (73.34 ± 0.07 g) were fed ARA diets (0.01 % and 0.86 %) for 60 d. Results demonstrated that a diet supplemented with 0.86 % ARA notably increased the progesterone (PROG) and prostaglandins (PGs) contents in hepatopancreas, as well as the thromboxane A2 (TXA2), estradiol (E2), and estriol (E3) contents in ovaries of E. sinensis (P < 0.05). Immunohistochemical analyses showed that 0.86 % ARA significantly promoted the expression of E2 receptor. Moreover, transcriptome sequencing revealed that 15 KEGG pathways, including the mTOR and FoxO signalling pathways and steroid biosynthesis, were enriched in the differentially expressed genes (DEGs) of crabs fed 0.86 % ARA. In conclusion, dietary 0.86 % ARA accelerated the deposition of ARA in the hepatopancreas and ovaries by regulating ARA synthesis and metabolism-related genes, then subsequently providing energy for ovarian development. Meanwhile, ARA promoted steroid hormone secretion and ovarian development-related pathways by facilitating the generation of PGs and ultimately promoted ovarian development in Chinese mitten crabs.
本研究设计了两个动物实验来评估花生四烯酸（ARA）对中华绒螯蟹卵巢发育的作用。在实验 1 中，雌性中华绒螯蟹（35.55 ± 0.02 g）被喂食七种实验日粮，每种日粮含有不同水平的 ARA（0.01 %、0.28 %、0.48 %、0.67 %、0.86 %、1.02 % 和 1.21 %），持续 120 d。86%的ARA能明显改善河蟹的生长性能，比0.67%的ARA更能明显提高性腺指数（GSI）、卵巢卵黄素（VTG）和卵黄素（Vn）的含量（P <0.05）。当日粮中 ARA 含量为 0.86 % 时，蛋的直径最大。此外，日粮 ARA 还通过增加磷脂酶 A2 (pla2)、环氧化酶-1 (cox-1)、环氧化酶-2 (cox-2)的相对表达量，显著影响肝胰腺和卵巢的脂肪酸组成和 ARA 含量、环氧化酶-2（cox-2）和 5-脂氧合酶（5-lox）的相对表达量增加，而脂肪酸合成酶（fas）、脂肪酸去饱和酶（fad6）、脂蛋白脂肪酶（lpl）和脂肪酸伸长酶（elo）的相对表达量减少（P <；0.05).根据 GSI 和卵直径，选择 0.86 % ARA 的用量来进一步研究卵巢发育的相关机制。在实验 2 中，雌蟹（73.34 ± 0.07 g）喂食 ARA 日粮（0.01 % 和 0.86 %）60 d。86%的 ARA 显著增加了中华绒螯虾肝胰腺中孕酮（PROG）和前列腺素（PGs）的含量，以及卵巢中血栓素 A2（TXA2）、雌二醇（E2）和雌三醇（E3）的含量（P <0.05）。免疫组化分析表明，0.86 % ARA 能显著促进 E2 受体的表达。此外，转录组测序显示，15 个 KEGG 通路（包括 mTOR 和 FoxO 信号通路以及类固醇生物合成）富集在喂食 0.86 % ARA 的螃蟹的差异表达基因（DEGs）中。总之，膳食 0.86 % ARA 可通过调节 ARA 合成和代谢相关基因，加速 ARA 在肝胰腺和卵巢中的沉积，进而为卵巢发育提供能量。同时，ARA通过促进PGs的生成，促进类固醇激素的分泌和卵巢发育相关途径，最终促进中华绒螯蟹的卵巢发育。
{"title":"Arachidonic acid promotes ovarian development by modulating lipid metabolism and steroid hormone secretion in Chinese mitten crab (Eriocheir sinensis)","authors":"Anran Wang , Xuran Liu , Jie Xu , Mengge Li , Dongxu Luo , Xingkong Ma , Yongtao Liu , Jiachun Ge , Qinghui Ai , Shuyan Miao","doi":"10.1016/j.aquaculture.2024.741844","DOIUrl":"10.1016/j.aquaculture.2024.741844","url":null,"abstract":"<div><div>In this study, two animal experiments were designed to assess the function of arachidonic acid (ARA) on the ovarian development of Chinese mitten crab (<em>Eriocheir sinensis</em>). In experiment 1, female crabs (35.55 ± 0.02 g) were fed seven experimental diets, each containing varying levels of ARA (0.01 %, 0.28 %, 0.48 %, 0.67 %, 0.86 %, 1.02 %, and 1.21 %) for 120 d. Dietary 0.48 %–0.86 % ARA significantly improved the growth performance of crabs, and more than dietary 0.67 % ARA significantly increased the gonadosomatic index (GSI), ovarian vitellogenin (VTG) and vitellin (Vn) contents (<em>P</em> < 0.05). The egg diameter was largest when dietary 0.86 % ARA. Moreover, dietary ARA also significantly affected the hepatopancreatic and ovarian fatty acid compositions and ARA content by increasing the relative expressions of phospholipase A2 (<em>pla2</em>), cyclooxygenase-1(<em>cox-1</em>), cyclooxygenase-2 (<em>cox-2</em>) and 5-lipoxygenase (<em>5-lox</em>), while decreasing the relative expressions of fatty acid synthase (<em>fas</em>), fatty acid desaturase (<em>fad6</em>), lipoprotein lipase (<em>lpl</em>) and fatty acid elongase (<em>elo</em>) (<em>P</em> < 0.05). Based on the GSI and egg diameter, the amount of 0.86 % ARA was selected to further investigate the mechanisms involved in ovarian development<em>.</em> In experiment 2, female crabs (73.34 ± 0.07 g) were fed ARA diets (0.01 % and 0.86 %) for 60 d. Results demonstrated that a diet supplemented with 0.86 % ARA notably increased the progesterone (PROG) and prostaglandins (PGs) contents in hepatopancreas, as well as the thromboxane A2 (TXA2), estradiol (E2), and estriol (E3) contents in ovaries of <em>E. sinensis</em> (<em>P</em> < 0.05). Immunohistochemical analyses showed that 0.86 % ARA significantly promoted the expression of E2 receptor. Moreover, transcriptome sequencing revealed that 15 KEGG pathways, including the mTOR and FoxO signalling pathways and steroid biosynthesis, were enriched in the differentially expressed genes (DEGs) of crabs fed 0.86 % ARA. In conclusion, dietary 0.86 % ARA accelerated the deposition of ARA in the hepatopancreas and ovaries by regulating ARA synthesis and metabolism-related genes, then subsequently providing energy for ovarian development. Meanwhile, ARA promoted steroid hormone secretion and ovarian development-related pathways by facilitating the generation of PGs and ultimately promoted ovarian development in Chinese mitten crabs.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741844"},"PeriodicalIF":3.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.aquaculture.2024.741847
Xu Zhang , Huan Wang , Zixuan Wang , Lipeng Shan , Yufeng Shen , Jinjun He , Lei Liu , Yang Hu , Jiong Chen
Aquaculture is increasingly important in providing a sustainable and diverse source of food and protein. However, disease outbreaks, particularly viral diseases, pose significant challenges to this sector. Micropterus salmoides rhabdovirus (MSRV) is a notable threat, especially to the widely cultivated largemouth bass, causing rapid onset of disease with no specific antiviral treatments available. This study aims to repurpose praziquantel, a widely used anthelmintic, for its antiviral properties against MSRV, leveraging its established safety and pharmacological profiles to expedite its application in aquaculture. Studies found that praziquantel at concentrations as high as 100 mg/L has no toxicity to EPC cells, inhibits MSRV replication by 82 %, and significantly reduces the cytopathic effects induced by viral infection. Further studies demonstrated that pretreatment with praziquantel enhanced the antiviral resistance of EPC cells, peaking at 24 h with an 82 % inhibition rate. The therapeutic effect was most pronounced when administered within 12 h post-infection, achieving a viral replication inhibition rate of over 70 %. Stability analysis found that despite a gradual decrease over 5 days, praziquantel retained significant antiviral activity, inhibiting MSRV infection by 79 % after 5 days. Additionally, praziquantel effectively reduced MSRV-induced apoptosis and preserved mitochondrial membrane potential (ΔΨm) in EPC cells, with flow cytometry confirming a 42 % reduction in apoptosis. In vivo experiments with largemouth bass confirmed the potential of praziquantel in aquaculture. At non-toxic doses (10 mg/L), praziquantel increased the survival rate of MSRV-infected fish from 13 % to 47 % over 15 days and achieved an 80 % virus inhibition rate. Praziquantel also showed promise in preventing horizontal transmission of MSRV in cohabitation experiments, with an inhibition rate of 52 % on viral replication in recipient fish. Our findings highlight the dual function of praziquantel as an anthelmintic and antiviral agent, providing a swift and efficient path to integrated disease management in aquaculture.
{"title":"New use of praziquantel as a broad-spectrum anti-parasitic agent in blocking MSRV infection","authors":"Xu Zhang , Huan Wang , Zixuan Wang , Lipeng Shan , Yufeng Shen , Jinjun He , Lei Liu , Yang Hu , Jiong Chen","doi":"10.1016/j.aquaculture.2024.741847","DOIUrl":"10.1016/j.aquaculture.2024.741847","url":null,"abstract":"<div><div>Aquaculture is increasingly important in providing a sustainable and diverse source of food and protein. However, disease outbreaks, particularly viral diseases, pose significant challenges to this sector. <em>Micropterus salmoides</em> rhabdovirus (MSRV) is a notable threat, especially to the widely cultivated largemouth bass, causing rapid onset of disease with no specific antiviral treatments available. This study aims to repurpose praziquantel, a widely used anthelmintic, for its antiviral properties against MSRV, leveraging its established safety and pharmacological profiles to expedite its application in aquaculture. Studies found that praziquantel at concentrations as high as 100 mg/L has no toxicity to EPC cells, inhibits MSRV replication by 82 %, and significantly reduces the cytopathic effects induced by viral infection. Further studies demonstrated that pretreatment with praziquantel enhanced the antiviral resistance of EPC cells, peaking at 24 h with an 82 % inhibition rate. The therapeutic effect was most pronounced when administered within 12 h post-infection, achieving a viral replication inhibition rate of over 70 %. Stability analysis found that despite a gradual decrease over 5 days, praziquantel retained significant antiviral activity, inhibiting MSRV infection by 79 % after 5 days. Additionally, praziquantel effectively reduced MSRV-induced apoptosis and preserved mitochondrial membrane potential (ΔΨm) in EPC cells, with flow cytometry confirming a 42 % reduction in apoptosis. In vivo experiments with largemouth bass confirmed the potential of praziquantel in aquaculture. At non-toxic doses (10 mg/L), praziquantel increased the survival rate of MSRV-infected fish from 13 % to 47 % over 15 days and achieved an 80 % virus inhibition rate. Praziquantel also showed promise in preventing horizontal transmission of MSRV in cohabitation experiments, with an inhibition rate of 52 % on viral replication in recipient fish. Our findings highlight the dual function of praziquantel as an anthelmintic and antiviral agent, providing a swift and efficient path to integrated disease management in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741847"},"PeriodicalIF":3.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.aquaculture.2024.741828
Mianyu Liu , Xupeng Li , Jie Kong , Xianhong Meng , Kun Luo , Juan Sui , Ping Dai , Jian Tan , Jiawang Cao , Baolong Chen , Hongjie Wang , Sheng Luan
Acute hepatopancreatic necrosis disease (AHPND) is a major bacterial disease caused by Vibrio parahaemolyticus (VPAHPND) that results in significant economic losses to Pacific white shrimp Litopenaeus vannamei aquaculture. Breeding of disease-resistant broodstocks is regarded as a fundamental strategy for solving the disease problem. This study evaluated the feasibility of genomic selection (GS) for enhancing the AHPND resistance of L.vannamei. Following the VPAHPND challenge, the survival time was utilized as a phenotypic measure to assess the AHPND resistance. The reference population consisted of 447 genotyped and 288 no-genotyped individuals with phenotypes. The candidate population consisted of 206 uninfected and genotyped individuals. The liquid chip “Yellow Sea Chip No.1” with 40 K single nucleotide polymorphism (SNP) generated genotypes for the reference and candidate populations. Heritabilities of survival time obtained using the average information REML method with a relationship matrix (A or H) and an animal model were 0.16 ± 0.06 and 0.22 ± 0.07, respectively. Predictive accuracies of pedigree-based best linear unbiased prediction (PBLUP), single-step genomic BLUP (ssGBLUP), and single-step Bayesian regression (ssBR) were evaluated using random, full-sib and between-family cross-validation. Compared to PBLUP, the predictive accuracies of ssGBLUP and ssBR for the survival time increased by 15.00 % and 67.50 % in random cross-validation, and 13.33 % and 53.33 % in full-sib cross-validation, respectively. All methods exhibited a reduction in predictive accuracy when moving from the random cross-validation or full-sib cross-validation to the between-family cross-validation. The predictive accuracies of Genomic BLUP (GBLUP) and Bayesian A (BayesA) were evaluated using full-sib cross-validation. Compared to GBLUP, the predictive accuracy of BayesA increased by 33.33 %. ssBR was used to predict the candidates' genomic estimated breeding values (GEBVs). 80 candidates with high GEBVs, 86 with medium GEBVs, and 40 with low GEBVs were selected to breed offspring with a spectrum of resistance levels. In the VPAHPND challenge test, the high resistance offspring had mean survival times that were 10.15 % longer than the moderate resistance offspring and 102.01 % longer than the low resistance offspring. This study validates the practicality and success of GS in improving resistance to AHPND in L.vannamei.
{"title":"Genomic selection of resistance to acute hepatopancreatic necrosis disease in Litopenaeus vannamei","authors":"Mianyu Liu , Xupeng Li , Jie Kong , Xianhong Meng , Kun Luo , Juan Sui , Ping Dai , Jian Tan , Jiawang Cao , Baolong Chen , Hongjie Wang , Sheng Luan","doi":"10.1016/j.aquaculture.2024.741828","DOIUrl":"10.1016/j.aquaculture.2024.741828","url":null,"abstract":"<div><div>Acute hepatopancreatic necrosis disease (AHPND) is a major bacterial disease caused by <em>Vibrio parahaemolyticus</em> (<em>VP</em><sub>AHPND</sub>) that results in significant economic losses to Pacific white shrimp <em>Litopenaeus vannamei</em> aquaculture. Breeding of disease-resistant broodstocks is regarded as a fundamental strategy for solving the disease problem. This study evaluated the feasibility of genomic selection (GS) for enhancing the AHPND resistance of <em>L.vannamei.</em> Following the <em>VP</em><sub>AHPND</sub> challenge, the survival time was utilized as a phenotypic measure to assess the AHPND resistance. The reference population consisted of 447 genotyped and 288 no-genotyped individuals with phenotypes. The candidate population consisted of 206 uninfected and genotyped individuals. The liquid chip “Yellow Sea Chip No.1” with 40 K single nucleotide polymorphism (SNP) generated genotypes for the reference and candidate populations. Heritabilities of survival time obtained using the average information REML method with a relationship matrix (A or H) and an animal model were 0.16 ± 0.06 and 0.22 ± 0.07, respectively. Predictive accuracies of pedigree-based best linear unbiased prediction (PBLUP), single-step genomic BLUP (ssGBLUP), and single-step Bayesian regression (ssBR) were evaluated using random, full-sib and between-family cross-validation. Compared to PBLUP, the predictive accuracies of ssGBLUP and ssBR for the survival time increased by 15.00 % and 67.50 % in random cross-validation, and 13.33 % and 53.33 % in full-sib cross-validation, respectively. All methods exhibited a reduction in predictive accuracy when moving from the random cross-validation or full-sib cross-validation to the between-family cross-validation. The predictive accuracies of Genomic BLUP (GBLUP) and Bayesian A (BayesA) were evaluated using full-sib cross-validation. Compared to GBLUP, the predictive accuracy of BayesA increased by 33.33 %. ssBR was used to predict the candidates' genomic estimated breeding values (GEBVs). 80 candidates with high GEBVs, 86 with medium GEBVs, and 40 with low GEBVs were selected to breed offspring with a spectrum of resistance levels. In the <em>VP</em><sub>AHPND</sub> challenge test, the high resistance offspring had mean survival times that were 10.15 % longer than the moderate resistance offspring and 102.01 % longer than the low resistance offspring. This study validates the practicality and success of GS in improving resistance to AHPND in <em>L.vannamei</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741828"},"PeriodicalIF":3.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.1016/j.aquaculture.2024.741831
Yanjing Chen , Zhou Chen , Min Xu , Lin Zhao
The increasing productivity of mariculture has earned it recognition as a promising strategy for meeting human protein demands. However, with the rapid development of the marine economy and the increasing demand for marine space, trade-offs between mariculture activities and other sea-use activities become more complex. Sustainable development of the mariculture industry requires the application of advanced spatial planning to identify suitable mariculture sites. This study examined the potential application of the Maxent-Marxan integrated method for mariculture spatial planning and developed a set of technical process for mariculture site selection, with the marine area of Jiangsu Province used as a case study. Based on environmental data for the Jiangsu Province marine area, the Maxent model was used to predict potentially suitable habitats for seven main mariculture species across four major categories. Subsequently, in combination with marine spatial usage data, the Marxan model was used to control costs and conduct zoning studies, ultimately establish an overall feasible planning scheme for mariculture in Jiangsu Province. The results indicated the total planned mariculture area of 9794.2 km2, representing 31.85 % of the marine area in Jiangsu Province. Identified suitable areas for shellfish, algae, fish, crustacean, and integrated shellfish-crustacean mariculture were 2147.32 km2, 2761.82 km2, 2135 km2, 1520.33 km2, and 1229.73 km2, respectively. The results confirmed the applicability and scientific validity of the Maxent model for predicting suitable habitats for individual mariculture species. The Maxent-Marxan integrated method can provide scientific support for the selection of mariculture sites for different types of mariculture species, and help decision-makers in coordinating the relationships between food demand, ecological conservation, and socio-economic development during the mariculture planning process, so as to make informed spatial planning decisions. This study provided a scientific reference for the application of the Maxent-Marxan integrated method to mariculture planning.
{"title":"Identification and delineation of mariculture area based on Maxent and Marxan: A case study in Jiangsu, China","authors":"Yanjing Chen , Zhou Chen , Min Xu , Lin Zhao","doi":"10.1016/j.aquaculture.2024.741831","DOIUrl":"10.1016/j.aquaculture.2024.741831","url":null,"abstract":"<div><div>The increasing productivity of mariculture has earned it recognition as a promising strategy for meeting human protein demands. However, with the rapid development of the marine economy and the increasing demand for marine space, trade-offs between mariculture activities and other sea-use activities become more complex. Sustainable development of the mariculture industry requires the application of advanced spatial planning to identify suitable mariculture sites. This study examined the potential application of the Maxent-Marxan integrated method for mariculture spatial planning and developed a set of technical process for mariculture site selection, with the marine area of Jiangsu Province used as a case study. Based on environmental data for the Jiangsu Province marine area, the Maxent model was used to predict potentially suitable habitats for seven main mariculture species across four major categories. Subsequently, in combination with marine spatial usage data, the Marxan model was used to control costs and conduct zoning studies, ultimately establish an overall feasible planning scheme for mariculture in Jiangsu Province. The results indicated the total planned mariculture area of 9794.2 km<sup>2</sup>, representing 31.85 % of the marine area in Jiangsu Province. Identified suitable areas for shellfish, algae, fish, crustacean, and integrated shellfish-crustacean mariculture were 2147.32 km<sup>2</sup>, 2761.82 km<sup>2</sup>, 2135 km<sup>2</sup>, 1520.33 km<sup>2</sup>, and 1229.73 km<sup>2</sup>, respectively. The results confirmed the applicability and scientific validity of the Maxent model for predicting suitable habitats for individual mariculture species. The Maxent-Marxan integrated method can provide scientific support for the selection of mariculture sites for different types of mariculture species, and help decision-makers in coordinating the relationships between food demand, ecological conservation, and socio-economic development during the mariculture planning process, so as to make informed spatial planning decisions. This study provided a scientific reference for the application of the Maxent-Marxan integrated method to mariculture planning.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741831"},"PeriodicalIF":3.9,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-30DOI: 10.1016/j.aquaculture.2024.741829
Quanquan Cao , Qin Zhang , Shiqi Li , Alkhateib Gaafar , Abdelgayed Metwaly Younes , Khalid Hussain Rind , Haifeng Liu , Jun Jiang
This review aims to summarize the current knowledge regarding the effects of various dietary additives on osmoregulation in aquaculture. It covers salt supplements, potassium supplements, myo-inositol supplements, metal ion supplements, and monosaccharides, emphasizing their importance in improving osmotic adjustment. The review provides an overview of the underlying mechanisms involved in osmoregulation and highlights the role of some specific dietary additives in promoting osmotic balance. Dietary salt and inorganic potassium directly provide ions for osmotic balance, while dietary metals, inositol, and monosaccharides can help mitigate the effects of osmotic stress and enhance the body's capacity for osmoregulation. Additionally, the addition of exogenous glucose not only provides energy required for osmoregulation but also acts as an osmolyte itself, contributing to osmoregulation. The review underscores the need for further research to better understand the interactions between dietary additives and osmoregulation in aquaculture. It also highlights the importance of considering species-specific requirements, optimal dosage levels, and the potential synergistic effects of combining multiple additives. Overall, carefully selected and properly administered dietary additives have the potential to improve osmoregulatory capacity, mitigate osmotic stress, and enhance the overall performance and welfare of cultured aquatic organisms. Future research should focus on elucidating the underlying mechanisms and optimizing the application of dietary additives to maximize their effectiveness in aquaculture systems.
{"title":"Effect of some feed additives on osmoregulation in aquatic animals","authors":"Quanquan Cao , Qin Zhang , Shiqi Li , Alkhateib Gaafar , Abdelgayed Metwaly Younes , Khalid Hussain Rind , Haifeng Liu , Jun Jiang","doi":"10.1016/j.aquaculture.2024.741829","DOIUrl":"10.1016/j.aquaculture.2024.741829","url":null,"abstract":"<div><div>This review aims to summarize the current knowledge regarding the effects of various dietary additives on osmoregulation in aquaculture. It covers salt supplements, potassium supplements, myo-inositol supplements, metal ion supplements, and monosaccharides, emphasizing their importance in improving osmotic adjustment. The review provides an overview of the underlying mechanisms involved in osmoregulation and highlights the role of some specific dietary additives in promoting osmotic balance. Dietary salt and inorganic potassium directly provide ions for osmotic balance, while dietary metals, inositol, and monosaccharides can help mitigate the effects of osmotic stress and enhance the body's capacity for osmoregulation. Additionally, the addition of exogenous glucose not only provides energy required for osmoregulation but also acts as an osmolyte itself, contributing to osmoregulation. The review underscores the need for further research to better understand the interactions between dietary additives and osmoregulation in aquaculture. It also highlights the importance of considering species-specific requirements, optimal dosage levels, and the potential synergistic effects of combining multiple additives. Overall, carefully selected and properly administered dietary additives have the potential to improve osmoregulatory capacity, mitigate osmotic stress, and enhance the overall performance and welfare of cultured aquatic organisms. Future research should focus on elucidating the underlying mechanisms and optimizing the application of dietary additives to maximize their effectiveness in aquaculture systems.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741829"},"PeriodicalIF":3.9,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-29DOI: 10.1016/j.aquaculture.2024.741820
Afaf N. Abdel Rahman , Sara T. Elazab , Elsayed M. Younis , Abdelwahab A. Abdelwarith , Tarek Khamis , Simon J. Davies , Rowida E. Ibrahim
Prunus armeniaca (apricot) kernel as a plant by-product, has garnered a lot of attention lately due to its biological and medicinal properties. This study represents a novel attempt to supplement Nile tilapia (Oreochromis niloticus) diets with the P. armeniaca kernels (PK) powder. This approach evaluated its effectiveness on growth, digestive/absorptive capabilities, economic efficiency, physiological/antioxidant status, and immune capacity. Additionally, the expression of nutrient transporters, lipid metabolism, and immune-relevant genes as well as the fish resistance to the Pseudomonas putida challenge were investigated. Fish (n = 160; 21.70 ± 0.22 g body weight) were allotted for a 3-month feeding trial into four treatments (n = 40 fish/treatment) in quadri-replicates (10 fish/replicate). The fish in each treatment were fed on a basal diet fortified with 0, 2, 4, and 8 g of PK/kg diet (PK0, PK2, PK4, and PK8), respectively. The outcomes revealed that all PK-enriched diets boosted growth indices (weight gain, specific growth rate, and protein efficiency ratio), growth hormone level, and intestinal enzyme activity (lipase and amylase). The PK-fortified diets improved intestinal morphometric indices (muscular coat thickness and villus width and length) and declined feed cost/kg gain. This enhancement was in a level-dependent manner with the PK8 treatment presenting the best results (P < 0.05). Up-regulation of the intestinal nutrient transporters-associated genes (solute carrier family 15 member 2, solute carrier family 9 member 3, and sodium-glucose cotransporter 1) was observed in PK-fed treatments. The expression of muscular lipid metabolism-relevant genes was modulated by the dietary PK, where lipid deposition-relevant genes (acetyl-coenzyme A carboxylase beta and sterol regulatory element-binding protein 1) down-regulated, while the lipolysis-relevant gene (carnitine palmitoyltransferase 1) up-regulated. The antioxidant-immune responses (glutathione peroxidase, catalase, total antioxidant capacity, total protein, globulin, serum bactericidal activity, nitric oxide, and complement3) were augmented by dietary PK inclusion with increasing PK dietary levels. Moreover, the expression of splenic immune-related genes (nuclear factor kappa beta, interferon-gamma, and Kelch-like ECH-associated protein1) was up-regulated by PK diets. Post-challenge with P. putida, the fish survival rate in PK8 treatment (87.50 %) was the highest followed by PK4 (81.25 %), and PK2 (75.00 %) treatments compared to PK0 treatment (56.25 %). Overall, it is advised to feed Nile tilapia with the PK-supplemented diets to enhance their growth, immune response, and resistance to P. putida. This provides an outline for the future use of these seed kernels for supporting the aquaculture sector.
{"title":"Dietary Prunus armeniaca augments antioxidant-immune-capacity, absorptive function, and growth and upregulates nutrient transporters and immune-regulatory genes of Oreochromis niloticus","authors":"Afaf N. Abdel Rahman , Sara T. Elazab , Elsayed M. Younis , Abdelwahab A. Abdelwarith , Tarek Khamis , Simon J. Davies , Rowida E. Ibrahim","doi":"10.1016/j.aquaculture.2024.741820","DOIUrl":"10.1016/j.aquaculture.2024.741820","url":null,"abstract":"<div><div><em>Prunus armeniaca</em> (apricot) kernel as a plant by-product, has garnered a lot of attention lately due to its biological and medicinal properties. This study represents a novel attempt to supplement Nile tilapia (<em>Oreochromis niloticus</em>) diets with the <em>P. armeniaca</em> kernels (PK) powder. This approach evaluated its effectiveness on growth, digestive/absorptive capabilities, economic efficiency, physiological/antioxidant status, and immune capacity. Additionally, the expression of nutrient transporters, lipid metabolism, and immune-relevant genes as well as the fish resistance to the <em>Pseudomonas putida</em> challenge were investigated. Fish (<em>n</em> = 160; 21.70 ± 0.22 g body weight) were allotted for a 3-month feeding trial into four treatments (<em>n</em> = 40 fish/treatment) in quadri-replicates (10 fish/replicate). The fish in each treatment were fed on a basal diet fortified with 0, 2, 4, and 8 g of PK/kg diet (PK0, PK2, PK4, and PK8), respectively. The outcomes revealed that all PK-enriched diets boosted growth indices (weight gain, specific growth rate, and protein efficiency ratio), growth hormone level, and intestinal enzyme activity (lipase and amylase). The PK-fortified diets improved intestinal morphometric indices (muscular coat thickness and villus width and length) and declined feed cost/kg gain. This enhancement was in a level-dependent manner with the PK8 treatment presenting the best results (<em>P</em> < 0.05). Up-regulation of the intestinal nutrient transporters-associated genes (solute carrier family 15 member 2, solute carrier family 9 member 3, and sodium-glucose cotransporter 1) was observed in PK-fed treatments. The expression of muscular lipid metabolism-relevant genes was modulated by the dietary PK, where lipid deposition-relevant genes (acetyl-coenzyme A carboxylase beta and sterol regulatory element-binding protein 1) down-regulated, while the lipolysis-relevant gene (carnitine palmitoyltransferase 1) up-regulated. The antioxidant-immune responses (glutathione peroxidase, catalase, total antioxidant capacity, total protein, globulin, serum bactericidal activity, nitric oxide, and complement3) were augmented by dietary PK inclusion with increasing PK dietary levels. Moreover, the expression of splenic immune-related genes (nuclear factor kappa beta, interferon-gamma, and Kelch-like ECH-associated protein1) was up-regulated by PK diets. Post-challenge with <em>P. putida</em>, the fish survival rate in PK8 treatment (87.50 %) was the highest followed by PK4 (81.25 %), and PK2 (75.00 %) treatments compared to PK0 treatment (56.25 %). Overall, it is advised to feed Nile tilapia with the PK-supplemented diets to enhance their growth, immune response, and resistance to <em>P. putida</em>. This provides an outline for the future use of these seed kernels for supporting the aquaculture sector.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741820"},"PeriodicalIF":3.9,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-29DOI: 10.1016/j.aquaculture.2024.741821
Morteza Yousefi , Hossein Adineh , Seyyed Morteza Hoseini , Seyed Amir Mehdi Hashemianfar , Evgeny Vladimirovich Kulikov , Nikolay Vladimirovich Petukhov , Tatyana Aleksandrovna Ryzhova
This study was designed to assess the effects of four feeds containing 0 (CTL), 25 mg/kg (LN25), 50 mg/kg (LN50) and 100 mg/kg (LN100) Laurus nobilis (LN) essential oil nano-liposomes (LN-NP) on Nile tilapia, Oreochromis niloticus. After 56 days of feeding with the above diets, growth performance and digestive enzyme activities determined in all treatments. Then, the fish were experimentally infected by Aeromonas hydrophila and their survival, antioxidant capacity, biochemical parameters and immune-related transcripts were measured, before and after the infection. The results showed that the growth rate and intestinal activities of amylase, lipase and protease were significantly higher in the LN50 and LN100 treatments than CTL. Post-infection survivals in the LN50 and LN100 treatments were similar and significantly higher than the CTL and LN25 treatments. Compared to the CTL treatment, the LN50 and LN100 treatments showed improved hepatic antioxidant parameters and lower malondialdehyde levels before and/or after infection. Infection significantly decreased plasma lysozyme and complement activities, but the LN50 and LN100 treatments had significantly higher activities before and after infection. These treatments had lower alanine aminotransferase and aspartate aminotransferase activities in plasma before and after infection, compared to the CTL treatment. The expression of tumor necrosis factor-alpha, interleukin-8 and interleukin-1 beta increased significantly in the LN50 and LN100 treatments, before and after infection, when compared to the CTL treatment. Based on the results, LN-NP at a dosage of 50 mg/kg is suitable for feeding Nile tilapia as it is a growth promoter and immunostimulator and increases the resistance of fish to A. hydrophila.
{"title":"Effects of Laurus nobilis essential oil nano-particles on growth performance, antioxidant and immune responses to bacterial infection in Nile tilapia, Oreochromis niloticus","authors":"Morteza Yousefi , Hossein Adineh , Seyyed Morteza Hoseini , Seyed Amir Mehdi Hashemianfar , Evgeny Vladimirovich Kulikov , Nikolay Vladimirovich Petukhov , Tatyana Aleksandrovna Ryzhova","doi":"10.1016/j.aquaculture.2024.741821","DOIUrl":"10.1016/j.aquaculture.2024.741821","url":null,"abstract":"<div><div>This study was designed to assess the effects of four feeds containing 0 (CTL), 25 mg/kg (LN25), 50 mg/kg (LN50) and 100 mg/kg (LN100) <em>Laurus nobilis</em> (LN) essential oil nano-liposomes (LN-NP) on Nile tilapia, <em>Oreochromis niloticus</em>. After 56 days of feeding with the above diets, growth performance and digestive enzyme activities determined in all treatments. Then, the fish were experimentally infected by <em>Aeromonas hydrophila</em> and their survival, antioxidant capacity, biochemical parameters and immune-related transcripts were measured, before and after the infection. The results showed that the growth rate and intestinal activities of amylase, lipase and protease were significantly higher in the LN50 and LN100 treatments than CTL. Post-infection survivals in the LN50 and LN100 treatments were similar and significantly higher than the CTL and LN25 treatments. Compared to the CTL treatment, the LN50 and LN100 treatments showed improved hepatic antioxidant parameters and lower malondialdehyde levels before and/or after infection. Infection significantly decreased plasma lysozyme and complement activities, but the LN50 and LN100 treatments had significantly higher activities before and after infection. These treatments had lower alanine aminotransferase and aspartate aminotransferase activities in plasma before and after infection, compared to the CTL treatment. The expression of <em>tumor necrosis factor-alpha</em>, <em>interleukin-8</em> and <em>interleukin-1 beta</em> increased significantly in the LN50 and LN100 treatments, before and after infection, when compared to the CTL treatment. Based on the results, LN-NP at a dosage of 50 mg/kg is suitable for feeding Nile tilapia as it is a growth promoter and immunostimulator and increases the resistance of fish to <em>A. hydrophila</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741821"},"PeriodicalIF":3.9,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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