Aquaculture最新文献

{"title":"The outer membrane vesicles of Vibrio parahaemolyticus elicit innate immune responses in mud crab (Scylla paramamosain)","authors":"Qian Sun ,&nbsp;Linying Yun ,&nbsp;Kaichun Hu ,&nbsp;Han Li ,&nbsp;Pengfei Li ,&nbsp;Yiyuan Lu ,&nbsp;Runwu Gao ,&nbsp;Ming Zhang ,&nbsp;Ngoc Tuan Tran ,&nbsp;Xiuli Chen ,&nbsp;Yongzhen Zhao ,&nbsp;Yueling Zhang ,&nbsp;Shengkang Li","doi":"10.1016/j.aquaculture.2025.742289","DOIUrl":"10.1016/j.aquaculture.2025.742289","url":null,"abstract":"<div><div>Outer membrane vesicles (OMVs) are extracellular nanostructures secreted by bacteria, which carry various virulence factors and play a pivotal role in the pathogenesis of bacterial infections. In this study, outer membrane vesicles derived from <em>Vibrio parahaemolyticus</em> (Vp-OMVs) were isolated to examine their effects on innate immunity in mud crabs (<em>Scylla paramamosain</em>). Characterization of the Vp-OMVs confirmed the presence of vesicles exhibiting typical OMV morphology and size. Vp-OMVs were internalized by host cells through clathrin-mediated endocytosis, leading to cytotoxicity, increased mortality, tissue vacuolation, and reduced cell viability. Furthermore, Vp-OMVs were found to contain lipopolysaccharide (LPS), which activates the Toll2 receptor, thereby initiating the Toll2 signaling pathway and promoting the expression of antimicrobial peptides. Additionally, internalized Vp-OMVs triggered inflammasome activation and mitochondrial dysfunction, resulting in inflammation and apoptosis. These findings highlight the critical role of Vp-OMVs in the pathogenesis of <em>V. parahaemolyticus</em> infections in mud crabs and offer valuable insights into potential therapeutic strategies for managing these infections.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742289"},"PeriodicalIF":3.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examination of the effects of avermectin-induced anxiety-like behavior on growth in the Chinese mitten crab, Eriocheir sinensis","authors":"Yi Huang ,&nbsp;Qiang Huang ,&nbsp;Shu Wu ,&nbsp;Zhiqiu Huang ,&nbsp;Yuhang Hong","doi":"10.1016/j.aquaculture.2025.742276","DOIUrl":"10.1016/j.aquaculture.2025.742276","url":null,"abstract":"<div><div>The widespread use of avermectin in both agriculture and aquaculture has raised concerns about its environmental impact on non-target aquatic species. In particular, the use of avermectin in rice-crab co-culture systems poses a significant risk with regards to the production of the Chinese mitten crab, <em>Eriocheir sinensis</em>, an ecologically and economically important species in China. Through chronic exposure experiments, this research assessed how sublethal concentrations of avermectin influence crab growth, food consumption, and neurobehavioral responses. Our results showed significant reductions in both food intake and overall growth in <em>E. sinensis</em> exposed to 2 and 20 μg/L of avermectin. Neurochemical analysis revealed elevated levels of serotonin (5-HT) and gamma-aminobutyric acid (GABA) in the brain of exposed crabs, while dopamine<!--> <!--> levels remained unchanged. Up-regulation of 5-HT and GABA receptor genes was observed under avermectin stress. In addition, oxidative stress was observed, with elevated levels of oxidative products in the brain. Behavioral tests linked neurochemical alterations in serotonin and GABA levels to marked anxiety-like behaviors, characterized by decreased locomotion and increased light avoidance. Importantly, administering 5-HT and GABA receptor antagonists alleviated anxiety-like behaviors. This treatment also reversed growth inhibition, underscoring the role of these neurotransmitters in mediating the toxic effects of avermectin. Our findings highlight the neurotoxic impact of avermectin on aquatic invertebrates and suggest potential intervention strategies to mitigate its adverse effects.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742276"},"PeriodicalIF":3.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-driven alterations in skin microbiota and biochemistry parameters of sturgeons","authors":"Jiehao Liu ,&nbsp;Shen Jin ,&nbsp;Yueping Zheng ,&nbsp;Fahim Ullah Khan ,&nbsp;Jianan Xu ,&nbsp;Houyong Fan ,&nbsp;Youji Wang ,&nbsp;Menghong Hu","doi":"10.1016/j.aquaculture.2025.742275","DOIUrl":"10.1016/j.aquaculture.2025.742275","url":null,"abstract":"<div><div>Water temperature constitutes a critical environmental factor that influences the physiological and biochemical processes in fish. Heat stress resulting from global warming negatively impacts the health of fish. Fish skin serves as a crucial defensive barrier for fish. However, limited information exists concerning the impact of heat stress on the skin health of cold-water fish. In this study we analyzed the influence of heat stress on multiple biochemical parameters and microbiota associated with the skin in the hybrid sturgeon (<em>Acipenser baerii</em> ♀ × <em>Acipenser schrencki</em> ♂). Sturgeons were exposed to low (16 °C), normal (22 °C), and high (28 °C) temperatures. The concentrations of total protein (TP), alanine aminotransferase (ALT), creatine kinase (CK), superoxide dismutase (SOD), GST (glutathione S-transferase), and glutathione peroxidase (GSH-Px) in skin mucus did not demonstrate significant alterations across all three groups (<em>p</em> &gt; 0.05). The concentrations of aspartate aminotransferase (AST), alkaline phosphatase (ALP), acid phosphatase (ACP), lactate dehydrogenase (LDH), catalase (CAT), malondialdehyde (MDA), and reduced glutathione (GSH) in mucus on day 5 were significantly elevated in the 28 °C group compared to the 16 °C group (<em>p</em> &lt; 0.05). The concentrations of lysozyme (LZM) in the 28 °C group demonstrated an upward trend, with a significant increase on day 14 (<em>p</em> &lt; 0.05). The concentrations of cortisol, AST, ALP, LDH, CAT, MDA, and GSH in the 28 °C group demonstrated an upward trend, with a significant increase on day 5 (<em>p</em> &lt; 0.05). Moreover, the sequencing of 16S rRNA revealed that heat stress exerts a significant influence on the diversity and community composition of the microbiota present in sturgeon skin mucus. The relative abundance of Actinobacteriota, Bacteroidota, Firmicutes, <em>Pseudochrobactrum</em>, <em>Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium</em>, and <em>Mesorhizobium</em> in the mucus decreased after heat stress, while the abundance of Fusobacteriota, <em>Enhydrobacter</em>, and <em>Cetobacterium</em> increased. This study revealed the potential ecophysiological responses of sturgeon fish under heat stress conditions, providing guidelines and a roadmap for conservation and aquaculture of sturgeons.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742275"},"PeriodicalIF":3.9,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ctenopharyngodon idella (grass carp) pVHL suppresses antiviral innate immunity by targeting MAVS for degradation upon GCRV infection","authors":"Wen Liu ,&nbsp;Xueyi Sun ,&nbsp;Chunchun Zhu ,&nbsp;Zixuan Wang ,&nbsp;Hongyan Deng ,&nbsp;Wuhan Xiao ,&nbsp;Xing Liu","doi":"10.1016/j.aquaculture.2025.742274","DOIUrl":"10.1016/j.aquaculture.2025.742274","url":null,"abstract":"<div><div>Grass carp reovirus (GCRV), a double-stranded RNA virus, has been identified as the causative pathogen of grass carp hemorrhagic disease (GCHD), which is one of the most severe diseases in freshwater aquaculture in China. Previously, we reported that the von Hippel–Lindau protein (pVHL) suppresses antiviral innate immunity during RNA virus infection by targeting the mitochondrial antiviral signaling (MAVS) protein for degradation. However, the role of pVHL in the innate immunity against GCRV is still not clear. In the present study, the VHL gene of grass carp was cloned, and the VHL gene was found to be conserved throughout the species. Using zebrafish as a model, we showed that disruption of <em>vhl</em> facilitates antiviral gene expression during GCRV infection. After GCRV infection or poly I:C stimulation, the mRNA level of <em>Ci</em>-VHL was significantly increased. Furthermore, <em>Ci</em>-pVHL interacts with <em>Ci</em>-MAVS, promotes degradation of <em>Ci</em>-MAVS, and inhibits <em>Ci</em>-MAVS-mediated interferon (IFN) activation. In addition, we screened 249 grass carp samples and identified three different types of single nucleotide polymorphisms (SNPs) within the <em>Ci</em>-VHL gene. The effect of each SNP on antiviral gene expression and virus replication following GCRV infection was also investigated. To conclude, the findings presented here may provide valuable insights regarding the function of pVHL in innate immunity against GCRV. Furthermore, VHL may serve as a promising target for the breeding of anti-GCRV grass carp strains.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742274"},"PeriodicalIF":3.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of 50 K liquid SNP array for blunt snout bream (Megalobrama amblycephala)","authors":"Li-Fei Luo ,&nbsp;Yao Wang ,&nbsp;Shi-Long Wang ,&nbsp;Yue Yu ,&nbsp;Yu-Ye Fu ,&nbsp;Chun-Hong Nie ,&nbsp;De-Wen Tang ,&nbsp;Ze-Xia Gao","doi":"10.1016/j.aquaculture.2025.742263","DOIUrl":"10.1016/j.aquaculture.2025.742263","url":null,"abstract":"<div><div>Blunt snout bream (<em>Megalobrama amblycephala</em>) is an economically important herbivorous fish native to China. However, the lack of high-efficient genotyping tools has impeded the genomic breeding programs for <em>M. amblycephala</em>. SNP array, an important genotyping platform, is crucial for implementing genomic selection. In this study, we employed the genotyping by liquid hybridization capture sequencing technology to create a multiple single-nucleotide polymorphism (mSNP) liquid array named Fangxin-I, which comprised 49,173 evenly distributed SNP loci across the 24 chromosomes of <em>M. amblycephala</em>. The array was designed for the comprehensive assessment of allelic variations across the genome of <em>M. amblycephala</em>. A total of 79.59 % of the array loci were of high-quality, with a genotyping success rate of 98.63 % across 52 individuals. The average genotype concordance rates reached 96.56 % between batches, and 99.40 % within batches. The comparison of the genotyping results between the SNP array and whole-genome re-sequencing indicated a genotype concordance of 98.32 %. Fangxin-I SNP array was subsequently employed for population genetics and genetic sex identification to validate its efficiency and accuracy. The analysis results indicated that the phylogenetic trees constructed for six populations of <em>M. amblycephala</em>, using the genotyping data from the Fangxin-I SNP array and the identified genomic loci from re-sequencing, reflected similar genetic relationships. Fangxin-I demonstrated a high accuracy in the genetic sex identification, affirming its effectiveness in both population genetics and genetic sex determination. Additionally, the array was used for genome-wide association study and identified the loci related to the resistance of <em>Aeromonas hydrophila</em> in <em>M. amblycephala</em> mainly on chromosomes 2, 4, 11 and 20. In summary, Fangxin-I SNP array will serve as an instrumental platform for high-quality genotyping, thereby facilitating the genomic selection breeding of <em>M. amblycephala.</em></div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742263"},"PeriodicalIF":3.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation on the antiviral activity of aloe emodin against Micropterus salmoides rhabdovirus in vitro and in vivo","authors":"Yao Wang ,&nbsp;Mengmeng Wang ,&nbsp;Zhiyang Lin ,&nbsp;Guangshuo Wang ,&nbsp;Yunlong Qin ,&nbsp;Mingzhu Liu ,&nbsp;Fei Ling ,&nbsp;Haifeng Jiang ,&nbsp;Tianqiang Liu ,&nbsp;Gaoxue Wang","doi":"10.1016/j.aquaculture.2025.742265","DOIUrl":"10.1016/j.aquaculture.2025.742265","url":null,"abstract":"<div><div><em>Micropterus salmoides</em> rhabdovirus (MSRV) is a highly pathogenic rhabdovirus that has caused significant economic losses in the aquaculture industry of largemouth bass. To date, there is no approved treatment for MSRV infection. In order to address this urgent need, virtual screening of the natural product monomer compound library targeting MSRV glycoprotein was conducted and the antiviral activity of 13 natural compounds identified in the screening was studied. Among these compounds, Aloe emodin (AE) exhibited high binding affinity and antiviral activity, inhibiting MSRV replication in grass carp ovary (GCO) cells with a half-maximal inhibitory concentration (IC₅₀) of 2.55 μM. Additionally, viral titers and cytopathic effects (CPE) were significantly reduced in the presence of AE. AE was demonstrated to directly destroy MSRV virions and inhibit their adsorption to GCO cells according to ultracentrifugation assay and viral binding assay. <em>In vivo</em> studies demonstrated that AE injection increased the survival rate of MSRV-infected largemouth bass by 63.3 %, which was 46.7 % higher than that of the virus group. RT-qPCR and pathological tissue section results demonstrated that AE treatment could significantly reduce viral loads in the liver, spleen, and kidney on days 1, 4, and 7 post-infection, and AE effectively mitigated tissue damage caused by MSRV infection. Circular dichroism (CD) spectroscopy and Isothermal titration calorimetry (ITC) showed that AE induced changes in the secondary structure of the MSRV glycoprotein and exhibited high affinity for the glycoprotein (Kd = 2.803 × 10⁻⁵ M, ΔG = −25.99 kJ/mol). These results suggested that AE was a natural product with potent anti-MSRV activity through direct targeting of the glycoprotein.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742265"},"PeriodicalIF":3.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomics and metabolomics uncover metabolites and pathways in American bullfrog (Aquarana catesbeiana) response to Elizabethkingia miricola infection","authors":"Peikui Yang ,&nbsp;Jiawei Lin ,&nbsp;Jude Juventus Aweya ,&nbsp;Qiongjun Zhao ,&nbsp;Yuzhong Zheng ,&nbsp;Xianghui Zou ,&nbsp;Yanjie Sun ,&nbsp;Yaqun Liu ,&nbsp;Shixiong Zhan","doi":"10.1016/j.aquaculture.2025.742262","DOIUrl":"10.1016/j.aquaculture.2025.742262","url":null,"abstract":"<div><div><em>Elizabethkingia miricola</em> is a widespread zoonotic bacterium capable of causing diseases in both humans and animals. A comprehensive understanding of the host response mechanisms to <em>E. miricola</em> infection is critical for the development of effective control strategies. In this study, American bullfrogs (<em>Aquarana catesbeiana</em>) were experimentally infected with <em>E. miricola</em>, and transcriptomic and metabolomic changes in the liver and spleen were analyzed at 0, 1, 2, and 3 days post-infection. A total of 3818 and 4166 differentially expressed genes (DEGs) were identified in the liver and spleen, respectively, along with 518 and 464 differentially abundant metabolites (DAMs). Short time-series expression miner (STEM) analysis revealed six and seven significant gene expression patterns in the liver and spleen, respectively, with alterations in numerous immune and lipid metabolism pathways identified by gene set enrichment analysis (GSEA). The metabolomic data showed dysregulation of 86 pathways in the liver and 75 in the spleen, predominantly involved in lipid, amino acid, and carbohydrate metabolism. Additionally, the IL-17 signaling pathway and glycerophospholipid pathway were highlighted as pivotal immune and antibacterial defense pathways in bullfrogs through KEGG enrichment and STEM analysis. Notably, the multi-omics study uncovered a complex regulatory network and highlighted stage-specific immune and metabolic responses in bullfrogs during <em>E. miricola</em> infection, providing valuable insights into their defense mechanisms.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742262"},"PeriodicalIF":3.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic selection for selective breeding of tiger pufferfish (Takifugu rubripes) for integration with surrogate broodstock technology","authors":"Sho Hosoya ,&nbsp;Sota Yoshikawa ,&nbsp;Masaomi Hamasaki ,&nbsp;Tomomi Kaneko ,&nbsp;Kiyoshi Kikuchi","doi":"10.1016/j.aquaculture.2025.742266","DOIUrl":"10.1016/j.aquaculture.2025.742266","url":null,"abstract":"<div><div>Aquacultural breeding and production of many commercially valuable fish species have been advanced by recent biotechnological breakthroughs and their integration. Here, we report our selective breeding program with the objectives of producing an elite all male population of the tiger pufferfish (<em>Takifugu rubripes</em>) that uses a combination of a genomic selection program and surrogate broodstock technology, a state-of-the-art form of reproductive engineering. In this species, the testis (shirako; marketable size &gt;100 g) is regarded as a favored delicacy and has the highest price of the edible parts; by contrast, the ovary is poisonous. In 2016, we initiated a genomic selection program with the aim of generating the tiger pufferfish populations in which more than 80 % of the males have testes larger than marketable size in December, when the fish are approximately 20 months old and the price is at its highest. The selection program started with commercially farmed individuals (F₀ generation) with mean testes weight (TW) of 67.6 g. Two cycles of selective breeding were carried out using TW and standard length; selected individuals were crossed in a partial factorial manner without increasing genetic relatedness between parents. An increase in mean TW to 94.2 g was found in the F₁ generation and to 117.5 g in the F₂ generation. The proportion of individuals with a TW larger than 100 g increased from 34.0 % in the F₀ generation to 42.9 % in the F₁ generation and 61.7 % in the F₂ generation. The narrow sense heritability estimated from single nucleotide polymorphism (SNP) markers was still high for each trait (<em>h</em>^<em>2</em> = 0.6–0.7) in the F2 generation. Large variations were observed in the values of both phenotypes and genomic estimated breeding values of the selected individuals of this generation. In addition, pedigree information reconstructed from the SNPs showed that F₂ individuals had similar genetic diversity to the F₀ generation. These results indicate that the selection scheme has been effective to date and that the selected population still has the potential for further genetic improvements in these traits. The revenue from the sale of testes could be doubled by production of all-male populations. The generation of such all-male populations can be facilitated through surrogate broodstock technology even for a species such as the tiger pufferfish in which conventional sex control techniques such as hormone administration do not work well.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742266"},"PeriodicalIF":3.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whey protein hydrolysate from camel mitigates hypoxia-induced renal and gill damage in Nile Tilapia: Effects on stress, HIF-α, apoptosis, inflammation, and metabolic pathways","authors":"Areej A. Eskandrani ,&nbsp;Nawal Alsubaie Alsubaie ,&nbsp;Ahmed Abdulwahab Bawahab ,&nbsp;Yasmina M. Abd-Elhakim ,&nbsp;Rowida E. Ibrahim ,&nbsp;Tarek Khamis ,&nbsp;Ali Osman ,&nbsp;Mohamed M.M. Metwally ,&nbsp;Badriyah S. Alotaibi ,&nbsp;Amany Abdel-Rahman Mohamed","doi":"10.1016/j.aquaculture.2025.742256","DOIUrl":"10.1016/j.aquaculture.2025.742256","url":null,"abstract":"<div><div>Hypoxia is among the most prevailing stressors in aquaculture and exerts negative impacts on fish health and metabolism; hence, the search for dietary strategies that mitigate such impacts has been of prime importance. In the present work, chronic daily periodic hypoxic stress on <em>Oreochromis niloticus</em> was induced, together with the protective role of camel whey protein hydrolysate added at 75 g/kg in the diet. The present study has focused on kidney function, serum ions, cortisol, glucose, and markers of oxidative stress. Expression of hypoxia-inducible factor (<em>HIF-α)</em> and inflammation-related genes in the kidney and key genes involved in metabolic and ion-regulatory functions in the gills were assessed. Fish was divided into four groups: a control group maintained in normoxic conditions with a basal diet; a hypoxic group subjected to daily 6-h hypoxic periods at DO ∼1.7 mg/L with the basal diet; a CW group fed camel whey protein hydrolysate under normoxic conditions; and a combined HPX + CW, which received CW-replaced diet (75 mg/kg diet) during HPX conditions. The hypoxic conditions were achieved by stopping aeration and covering the aquaria with a plastic film daily for 6 h, while the temperature and ammonia levels in the water were regularly checked during the experimental period of four successive weeks. The results revealed renal tissue damage with increased levels of oxidative biomarkers. On the molecular level, down-regulation of <em>SDH</em>; and the up-regulation expression of hypoxia and inflammation-related genes (<em>HIF-α</em>, Interleukin-1β <em>(IL-1β</em>), Collagen, type I, alpha 1(<em>COL1A1</em>), and Transforming factor beta-1 (<em>TGF-β1</em>)) in the kidney. Also, histopathological alterations were observed including, nephropathic alterations and hyperplasia of gill epithelium, with enhancement in the immunoreactivity to TNF-α and Caspase-3. In turn, CW supplementation significantly improved all these parameters and hence can be used as an anti-stress dietary supplement to enhance resilience against hypoxic challenges.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742256"},"PeriodicalIF":3.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First genome-wide association and genomic prediction of ammonia‑nitrogen tolerance in tiger pufferfish (Takifugu rubripes)","authors":"Yong Chi ,&nbsp;Clémence Fraslin ,&nbsp;Robert Mukiibi ,&nbsp;Yuanri Hu ,&nbsp;Weidong Li ,&nbsp;Diego Robledo ,&nbsp;Songlin Chen ,&nbsp;Yangzhen Li","doi":"10.1016/j.aquaculture.2025.742260","DOIUrl":"10.1016/j.aquaculture.2025.742260","url":null,"abstract":"<div><div>Ammonia‑nitrogen stress is a significant threat to the growth and survival of tiger pufferfish (<em>Takifugu rubripes</em>), especially in high-density stocking integrated aquaculture systems. This study aimed to decipher the genetic background of ammonia‑nitrogen stress tolerance via heritability estimation and identification of variants associated with this stress via genome-wide association studies (GWAS). We also assessed the potential of applying genomic selection (GS) to improve this trait in <em>T. rubripes</em>. A customized a low-density 20 K SNP array was used to genotype 780 fish, and low-density genotypes were imputed to a high-density genotype dataset comprising over 1.3 million SNPs. The heritability of ammonia‑nitrogen tolerance was estimated as 0.20 and 0.15 and 0.21 for pedigree, low-density and high-density SNP markers panels, respectively. A suggestively significant quantitative trait locus (QTL) was detected on chromosome 8, containing 16 significant SNPs within a 70.0 kb region. Notably, two candidate genes, <em>fgf11A</em> and <em>serinc5</em>, were associated with ammonia‑nitrogen stress tolerance. Use of different genomic prediction models to estimate genetic merit of selection candidates showed improvement in accuracy of breeding value estimation by at least 57 % compared to traditional pedigree-based methods. Interestingly, the prediction remained significantly high even with the lowest number of panel markers (500 markers). These findings highlight the potential of GS to enhance ammonia‑nitrogen stress tolerance in <em>T. rubripes</em> breeding programs, thus contributing to the sustainability and productivity of the species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742260"},"PeriodicalIF":3.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}