Aquaculture最新文献

{"title":"Retinol binding protein 4 (RBP4): a novel orexigenic factor triggers hypothalamic appetite regulators via AMPK /AKT/mTOR signaling to promote feeding in Siberian sturgeon (Acipenser baerii)","authors":"Ni Tang ,&nbsp;Yingzi Li ,&nbsp;Yixiao Xiong ,&nbsp;Zhaoxiong Yu ,&nbsp;Fei Yang ,&nbsp;Xueling Zhang ,&nbsp;Xin Zhang ,&nbsp;Bo Zhou ,&nbsp;Zhiqiong Li ,&nbsp;Bin Wang","doi":"10.1016/j.aquaculture.2026.743670","DOIUrl":"10.1016/j.aquaculture.2026.743670","url":null,"abstract":"<div><div>Retinol binding protein 4 (RBP4), primarily recognized as an adipokine in mammals, is involved in energy metabolism and feeding regulation, with potential functions related to the hypothalamus. However, the role of RBP4 on feeding regulation remains unknown. Here, the study cloned the Siberian sturgeon (<em>Acipenser baerii</em>) <em>rbp4</em> CDS sequence which was 594 base pairs (bp) and encoded 197 amino acids. The Rbp4 protein of Siberian sturgeon exhibited low identity when compared with that of other vertebrates except for other sturgeons. Abundant expression of <em>rbp4</em> mRNA was detected in the liver of Siberian sturgeon, suggesting that Rbp4 might act as a hepatokine. In the liver, feeding elevated <em>rbp4</em> expression at 1 h (+1 h) and 3 h (+3 h) after feeding, while fasting for 10 days decreased its expression. Additionally, in the hypothalamus, feeding reduced the <em>rbp4</em> mRNA level at +3 h post-feeding, and fasting for 10 day elevated it, suggesting that rbp4 might have an orexigenic function in the central nervous system. This study expressed and purified the mature domain of the Siberian sturgeon RBP4 (SsRBP4) protein using a pET32a vector in prokaryotic cells. An intraperitoneal (<em>i.p.</em>) injection of SsRBP4 significantly promoted food intake during 1-3 h and 3-6 h periods post-injection, but not during 0-1 h period. Meanwhile, an <em>i.p.</em> injection of SsRBP4 stimulated the expression of orexigenic genes (<em>npy</em>, <em>agrp</em>, <em>apelin</em>) and inhibited the expression of anorexigenic gene (<em>cart</em>) in the hyphothalamus at 3 h post-injection, but did not affect the expression of <em>pomc</em> and <em>insulin</em>. Injection of SsRBP4 promoted hyphothalamic <em>cb1r</em> and <em>ampkα1</em> expression, but inhibited the expression of <em>lepr</em>, <em>mc4r</em>, <em>ampkα2</em>, <em>akt</em>, <em>mtor</em> and <em>s6k1</em>. This study provides the first evidence that Rbp4 acts as a potent orexigenic hepatokine in the Siberian sturgeon. Critically, the ability of peripherally administered SsRBP4 to stimulate food intake and modulate key hypothalamic appetite regulators highlights its significant potential as a target for developing novel strategies to enhance feeding and growth in this economically valuable aquaculture species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743670"},"PeriodicalIF":3.9,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vertical transmission of core microbiota from maternal tissues to early developmental stages in the golden cuttlefish Sepia esculenta","authors":"Juanwen Yu ,&nbsp;Siqing Chen ,&nbsp;Tianshi Zhang ,&nbsp;Jinchao Zhu ,&nbsp;Qing Chang ,&nbsp;Li Bian","doi":"10.1016/j.aquaculture.2026.743674","DOIUrl":"10.1016/j.aquaculture.2026.743674","url":null,"abstract":"<div><div>Microbiota play critical roles in the early development and health of marine invertebrates, yet microbial transmission and assembly across life stages remain poorly understood in cephalopods. The golden cuttlefish (<em>Sepia esculenta</em>), an important cephalopod in East Asia, suffers high larval mortality, potentially linked to microbiome instability. Here, we investigated vertical microbial transmission, stage-specific microbial succession, and functional roles of core microbiota during early development of <em>S. esculenta</em>. Using high-throughput 16S rRNA sequencing and culture-based methods, we profiled the microbiota across maternal tissues, as well as embryonic stages and post-hatching larvae (<em>n</em> = 79). Two vertically transmitted core genera, <em>Vibrio</em> and <em>Pseudomonas</em>, persisted from maternal organs to offspring and met prevalence and abundance thresholds (NorCI &gt; 0.1). Stage-specific microbial shifts were also observed, with <em>Aureisphaera</em> dominating early embryonic stages and <em>Mycoplasma</em>, <em>Acinetobacter</em>, and <em>Croceibacter</em> emerging after hatching. Functional predictions revealed enrichment of redox-related pathways in embryos and metabolism-associated functions in larvae. Source tracking demonstrated organ-specific microbial transmission, with skin microbiota and environmental inputs. Culture-based isolation successfully retrieved 176 ASVs and improved taxonomic resolution for 92% of them. Three isolates, <em>Bacillus</em>, <em>Pseudoalteromonas</em>, and <em>Sulfitobacter</em> spp., exhibited antagonistic activity against <em>Vibrio tubiashii</em>, suggesting potential probiotic applications. Overall, our results demonstrate that early-life microbiome establishment in <em>S. esculenta</em> involves both maternal inheritance and stage-specific acquisition, providing insights for microbiome-informed strategies to enhance larval health in cephalopod aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743674"},"PeriodicalIF":3.9,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient changes from aquaculture history and the environmental implications in a typical open coast of the East China Sea","authors":"Longqiang Peng ,&nbsp;Bing Deng ,&nbsp;Guosen Zhang ,&nbsp;Zhuoyi Zhu ,&nbsp;Xin Sun ,&nbsp;Zengjie Jiang ,&nbsp;Jinzhou Du ,&nbsp;Chengxu Zhou ,&nbsp;Yuanyuan Chen ,&nbsp;Yufeng Chen ,&nbsp;Lijun Hou ,&nbsp;Jing Zhang","doi":"10.1016/j.aquaculture.2026.743665","DOIUrl":"10.1016/j.aquaculture.2026.743665","url":null,"abstract":"<div><div>Nutrient changes derived from high-resolution sediment records along a typical open coast of the East China Sea were analyzed to assess environmental variations associated with aquaculture practices over recent decades. Biogenic components and stable isotopes significantly fluctuated in the sediment profiles, suggesting substantial environmental changes since the 1930s. Slight increases in total nutrient contents and carbon stable isotopes (δ¹³C_org) were observed from the 1930s to the 1970s, likely due to increasing terrestrial nutrient inputs resulting from population growth within river watersheds. From the 1970s to the 1980s, δ¹⁵N values slightly decreased, suggesting an influx of anthropogenic nutrients primarily originating from chemical fertilizers. A minor decline was observed in total nitrogen and organic phosphorus, as well as total organic carbon contents during this period, coinciding with the expansion of kelp (<em>Saccharina japonica</em>) cultivation. Since the 1990s, nitrogen and phosphorus have substantially increased by over 31% and 22%, respectively, along with a notable shift toward heavier δ¹⁵N values attributed to feed inputs. Additionally, fluctuations in δ¹³C_org indicate that feed-based aquaculture has profoundly altered both nutrient structure and composition along the Lianjiang coast. The annual nitrogen and phosphorus loadings absorbed by aquaculture were calculated to be approximately 14,592 ± 3812 and 1848 ± 762 t in 2020, respectively. Meanwhile, annual nitrogen and phosphorus discharges from feed-based farming amounted to approximately 17,038 ± 4465 t and 3343 ± 612 t, respectively. Nevertheless, the net N loadings from aquaculture in Lianjiang were one order of magnitude lower than those for discharge or absorption. Kelp accounted for over 86% of nutrient removal in Lianjiang, despite its cultivation period lasting only 4–6 months each year. This suggests that significant net absorption occurs during the kelp cultivation season, which mitigates nutrient loads from adjacent coastal regions. Conversely, during non-cultivation seasons, prominent net discharge of nutrients was expected to affect adjacent coastal areas. Thus, seasonal variations in kelp cultivation practices appear to modify nutrient cycles in the adjacent marine environments.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743665"},"PeriodicalIF":3.9,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bolstering shrimp resilience against AHPND: Biofloc reduce Vibrio parahaemolyticus population, affects microbial composition, and increase Penaeus vannamei shrimp growth and resistance","authors":"Muhamad Gustilatov ,&nbsp;Widanarni ,&nbsp;Julie Ekasari ,&nbsp;Pande Gde Sasmita Julyantoro ,&nbsp;Diana Elizabeth Waturangi","doi":"10.1016/j.aquaculture.2026.743667","DOIUrl":"10.1016/j.aquaculture.2026.743667","url":null,"abstract":"<div><div>The acute hepatopancreatic necrosis disease (AHPND) caused by <em>Vibrio parahaemolyticus</em> infection in the <em>Penaeus vannamei</em> shrimp culture causes significant losses to the aquaculture industry. This study aims to evaluate the influence of biofloc on the composition of microbiota in the shrimp rearing media and digestive tract, growth performance, and resistance of shrimp to <em>V. parahaemolyticus</em> challenge. Pacific white shrimp postlarvae (PL 30) with an average body weight of 0.66 ± 0.02 g were reared in 12 units of glass aquaria (water volume 22 L) at a density of 1500 m⁻³ for 28 days. The treatments tested in this study were a biofloc system with molasses addition at a C/N ratio of 10 and treatment without biofloc, and each followed by a challenge test by <em>V. parahaemolyticus</em> immersion at a bacterial density of 10⁵ CFU mL⁻¹ on the 15th day of culture. The results showed microbial composition of the water and shrimp gut microbiota. Biofloc treatment shows ability to reduce the relative abundance of <em>V. parahaemolyticus</em> in the shrimp gut despite being challenged with <em>V. parahaemolyticus</em>. Furthermore, biofloc significantly (<em>p</em> &lt; 0.05) improved growth performance (absolute weight gain, specific growth rate, and feed conversion ratio), digestive enzyme activity, and increased the resistance of shrimp challenged with <em>V. parahaemolyticus</em>. The conclusion of this study is that biofloc can modulate the composition of water and shrimp gut microbiota while decreasing number of <em>V. parahaemolyticus</em>, improve growth performance, and protect shrimp from AHPND disease.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743667"},"PeriodicalIF":3.9,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing intensive culture of Macrobrachium rosenbergii in a pond-scale biofilm reactor with polypropylene-fiber brushes: Performance and microbial mechanisms","authors":"Junkai Lu ,&nbsp;Xinming Gao ,&nbsp;Lihui Zhang ,&nbsp;Yin Chen ,&nbsp;Zheke Cen ,&nbsp;Danyi Shen ,&nbsp;Yaoping Lv","doi":"10.1016/j.aquaculture.2026.743672","DOIUrl":"10.1016/j.aquaculture.2026.743672","url":null,"abstract":"<div><div>The giant freshwater prawn (<em>Macrobrachium rosenbergii</em>) is a globally important aquaculture species, yet its intensive culture is constrained by toxic inorganic nitrogen accumulation. Effective nitrogen removal is critical for sustainable, intensive aquaculture. Biofilm-based systems offer a promising solution through microbial-driven nitrogen transformation, but their application in <em>M. rosenbergii</em> remains limited. In this study, a novel biofilm reactor system was developed using polypropylene-fiber brush carriers to facilitate biofilm formation. Compared with conventional aquaculture practices, the brush-based biofilm system significantly improved water quality and enhanced the yield of <em>M. rosenbergii</em>. A robust and stable nitrifying biofilm, dominated by the genus <em>Nitrospira</em>, was established on the brush carriers. In addition, the three-dimensional, high-surface-area brush carriers provided abundant niches for microbial colonization, supporting efficient biofilm development and ecological stability. These findings demonstrate that the engineered biofilm reactor with brush carriers is an effective strategy to control nitrogenous pollutants and improve prawn production, providing a practical pathway toward sustainable freshwater aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743672"},"PeriodicalIF":3.9,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stress triggers gill damage in hybrid sturgeon (A. baerii♀ × A. schrenckii♂) via synergistic effects on apoptosis, microbial dysbiosis, physical barrier dysfunction, and inflammatory response","authors":"Shiyong Yang ,&nbsp;Datian Li ,&nbsp;Yuan Gao ,&nbsp;Zhen Wei ,&nbsp;Yinlin Xiong ,&nbsp;Yan Liu ,&nbsp;Shihui Yi ,&nbsp;Xinrui Lyu ,&nbsp;Dandan Xi ,&nbsp;Yunkun Li ,&nbsp;Jiayun Wu ,&nbsp;Xiaoshuang Chen ,&nbsp;Fushi Tang ,&nbsp;Qi Tang ,&nbsp;Xiaogang Du","doi":"10.1016/j.aquaculture.2026.743668","DOIUrl":"10.1016/j.aquaculture.2026.743668","url":null,"abstract":"<div><div>Heat stress poses a significant threat to cold-water fish, such as sturgeon, with the gill being a primary target organ. However, the comprehensive pathological mechanisms remain poorly understood. In this study, the impact of heat stress (28 °C) on the gills of hybrid sturgeon (<em>A. baerii × A. schrenckii</em>) was investigated. Heat stress induced severe tissue damage, inflammatory cell infiltration, and elevated reactive oxygen species levels. Transmission electron microscopy revealed ultrastructural alterations, including chromatin condensation, apoptotic vesicles, mitochondrial swelling, and endoplasmic reticulum dilation. Furthermore, heat stress triggered endoplasmic reticulum stress, as evidenced by the upregulation of genes in the PERK pathway (<em>perk</em>, <em>eif2-α</em>, <em>atf4</em> and <em>chop</em>) and IRE1 pathway (<em>ire1</em>, <em>xbp1</em> and <em>chop</em>) as well as the elevated expressions of <em>hsp70</em>, <em>hsp90</em>, and HSP70 protein (leading to increased apoptosis). Concurrently, the expressions of tight junction (<em>cldn</em>, and <em>ocln</em>) and osmoregulatory genes (<em>aqp3</em>, <em>nhe3</em>, and <em>clc-2</em>) related to the physical barrier were dysregulated under heat stress. Further, 16S rDNA sequencing revealed microbial dysbiosis, which is characterized by an increase in the abundance of potential pathogens (<em>Rhizobiales Incertae Sedis</em> and <em>norank_o_PeM15</em>) and a decrease in the abundance of beneficial bacteria (<em>Rhodococcus erythropolis</em>); these results correlated with the activation of genes in the inflammatory pathways (<em>tlr1</em>, <em>tlr9</em>, <em>nod1</em>, <em>nod2</em>, and <em>nf-κb</em>) and an increase in the pro-inflammatory cytokine levels (<em>tnf-α, il-6</em>, and <em>il-8</em>). In summary, our findings demonstrate that heat stress exacerbates gill damage in hybrid sturgeon by promoting endoplasmic reticulum stress-mediated apoptosis, disrupting microbial homeostasis, compromising physical barrier, and inducing intense inflammatory responses.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743668"},"PeriodicalIF":3.9,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grafting surgery elicits systemic changes in antioxidant and immune responses in red abalone (Haliotis rufescens)","authors":"Alejandro Rojas-Figueroa ,&nbsp;Carlos Angulo ,&nbsp;Andrés Granados-Amores ,&nbsp;Gabriel Correa-Reyes ,&nbsp;Pedro E. Saucedo","doi":"10.1016/j.aquaculture.2026.743664","DOIUrl":"10.1016/j.aquaculture.2026.743664","url":null,"abstract":"<div><div>Grafting surgery, a key step in pearl culture process, can trigger physiological, antioxidant, and immune responses in the host mollusk. Adult red abalone (<em>Haliotis rufescens</em>) were subjected to four experimental treatments: (1) anesthesia only (AN); (2) anesthesia plus a surgical incision (IN) in the abdominal wall without mantle grafting; (3) anesthesia, incision, and placement of an autograft (AT); and (4) anesthesia, incision, and placement of an allograft (AL). The control group (CT) consisted of unmanipulated abalone. Hemolymph samples were collected during the recovery phase at 2, 4, 24, and 48 h post-grafting. In both the cell-free hemolymph fraction (CFH) and lysed hemocytes (LH), total protein (TP) content, superoxide dismutase (SOD), catalase (CAT), and myeloperoxidase (MPO) activities, as well as nitric oxide (NO) production and total hemocyte count (THC), were quantified. SOD activity increased across all treatments at 48 h, whereas CAT activity was elevated only in the AL group at 2 and 4 h and in the AT group at 24 and 48 h. THC increased rapidly in the AL group (2 h), followed by increases in the IN and AT groups (4 h), with gradual declines observed by 48 h. Immunological parameters (MPO and NO) were detected primarily in the AL group at nearly all time points. The results of this study demonstrate marked modulation of antioxidant and immunological parameters at both humoral and cellular levels in response to grafting, particularly in the AL and AT groups. Importantly, the immunological activation observed in the AL group was confined to the early post-operative period (48 h) and did not compromise survival, as no mortality was recorded in any experimental treatment. These findings support the feasibility of allografting as part of pearl culture protocols in <em>H. rufescens</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743664"},"PeriodicalIF":3.9,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary D-mannose maintains intestinal microbiota balance and alleviates dextrose sodium sulfate (DSS)-induced enteritis of juvenile grass carp (Ctenopharyngodon idella)","authors":"Chong Zhang ,&nbsp;Lin Feng ,&nbsp;Pei Wu ,&nbsp;Yang Liu ,&nbsp;Xiaowan Jin ,&nbsp;Hongmei Ren ,&nbsp;Xiaoqiu Zhou ,&nbsp;Weidan Jiang","doi":"10.1016/j.aquaculture.2026.743661","DOIUrl":"10.1016/j.aquaculture.2026.743661","url":null,"abstract":"<div><div>D-mannose, a biologically active monosaccharide, is pivotal for protein glycosylation and immune regulation, yet its role and underlying mechanisms in enhancing fish enteritis resistance remain inadequately characterized. This study aimed to investigate the effects of dietary D-mannose on gut microbiota structure and resistance to dextran sodium sulfate (DSS)-induced enteritis. All 1800 juvenile grass carp were randomly assigned to 6 treatment groups and fed diets supplemented with graded levels of D-mannose (0.52, 1.75, 3.02, 4.28, 5.50 and 6.78 g/kg) for 70 days, followed by a DSS-induced enteritis challenge. The results revealed that D-mannose improved gut microbial structure and short-chain fatty acid levels, suggesting a potential enhancement in enteritis resistance. In the DSS-induced enteritis model, D-mannose supplementation significantly reduced enteritis incidence and mitigated intestinal histopathological damage, as evidenced by improved epithelial cell arrangement and reduced lamina propria edema. Mechanistically, D-mannose enhanced intestinal antimicrobial capacity and modulated the balance of pro-inflammatory and anti-inflammatory cytokines, which was associated with the regulation of CD4⁺ T cell differentiation to maintain Th1/Th2 and Th17/Treg subset homeostasis. Additionally, D-mannose alleviated intestinal pyroptosis by suppressing the NLRP3 inflammasome pathway, which may be mediated by preserving lysosomal integrity and inhibiting cathepsin B activation. Collectively, these findings demonstrate that dietary D-mannose effectively enhances fish enteritis resistance and intestinal immune function, with mechanisms involving the modulation of gut microbiota, regulation of T cell differentiation, and inhibition of lysosome-dependent NLRP3 inflammasome-mediated pyroptosis. This study provides a theoretical basis and practical guidance for the application of D-mannose as a potential antibiotic alternative in aquafeeds to mitigate enteritis in grass carp.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743661"},"PeriodicalIF":3.9,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Summertime CO2 flux and its driving mechanisms in shellfish-dominated integrated multi-trophic aquaculture (IMTA) ponds","authors":"Chunpu Zhao,&nbsp;Anhao Wang,&nbsp;Wenhao Yin,&nbsp;Zhanfeng Liang,&nbsp;Ying Zeng,&nbsp;Kai Liao,&nbsp;Jilin Xu,&nbsp;Shuonan Ma","doi":"10.1016/j.aquaculture.2026.743663","DOIUrl":"10.1016/j.aquaculture.2026.743663","url":null,"abstract":"<div><div>The Integrated Multi-Trophic Aquaculture (IMTA) holds significant application value in pond aquaculture systems, yet the dynamics and drivers of water–air CO₂ fluxes within these systems remain unresolved. We hypothesize that pH and trophic status may act as key regulators of the CO₂ flux across the water-air interface within IMTA ponds. To verify this, we quantified in situ CO₂ fluxes and their drivers in five mariculture ponds that represent typical calcifying-bivalve (<em>Sinonovacula constricta</em>) IMTA systems. The results demonstrated that IMTA ponds generally functioned as a weak net sink of CO₂ at the water-air interface in summer. Four fluorescent dissolved organic matter (DOM) components—one humic-like and three protein-like—among which the terrestrial humic-like fluorophore (C2) dominated were identified by Excitation–emission matrix (EEM) spectroscopy combined with parallel factor analysis (PARAFAC). Further structural equation modeling (SEM) analysis revealed that trophic status (nitrogen and phosphorus concentrations) was the major environmental factor influencing DOM transformation and degradation, potentially regulating CO₂ fluxes indirectly by affecting humic substance accumulation and degradation. Water pH emerged as another major driver, directly affecting CO₂ speciation and seawater pCO₂ while indirectly modulating CO₂ fluxes through the modulation of bivalve calcification, as suggested by SEM (with a standardized effect size of −0.58). Here we systematically elucidate the summertime CO₂ sink and its underlying mechanisms in IMTA ponds, providing a theoretical framework for optimizing management practices—such as pH control and dynamic nutrient adjustment—to enhance the carbon-sequestration potential of marine aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743663"},"PeriodicalIF":3.9,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decay kinetics of white spot syndrome virus in seawater and sediment: Implications for reservoir-mediated transmission","authors":"Min Jae Kim ,&nbsp;Kwang Il Kim","doi":"10.1016/j.aquaculture.2026.743658","DOIUrl":"10.1016/j.aquaculture.2026.743658","url":null,"abstract":"<div><div>White spot syndrome virus (WSSV) is a major threat to shrimp aquaculture, and pond-based systems relying on coastal seawater exchange render seawater and sediment critical environmental reservoirs. This study aimed to characterize the decay kinetics of WSSV in these matrices and derive key parameters for reservoir-mediated transmission risk assessment. The viral extraction efficiency and viability of the recovered WSSV from seawater and sediment were evaluated using qPCR, viability qPCR (v-qPCR), and bioassays. Based on v-qPCR-derived time-course data, WSSV decay kinetics were modeled at different temperatures, and <em>T</em>_<em>90</em>/<em>T</em>_<em>99</em> (time required for 90% and 99% reductions, respectively) values were validated against experimental infectivity. WSSV persistence was inversely associated with temperature (30–4 °C), with more rapid decay observed in environmental seawater than in sediment. In seawater, <em>T</em>_<em>99</em> values ranged from 2.26 days at 30 °C to 19.10 days at 4 °C, with viable WSSV detectable for up to 42 days. In contrast, sediment exhibited markedly greater persistence, with <em>T</em>_<em>99</em> values reaching 84.55 days at 4 °C, and viable WSSV detectable for up to 90 days. A strong positive correlation (<em>r</em> &gt; 0.96) between the decay kinetics model–derived <em>T</em>_<em>90</em>/<em>T</em>_<em>99</em> values and infectivity reduction time points supports the validity of <em>T</em>_<em>90</em>/<em>T</em>_<em>99</em> as a reliable indicator of viable WSSV decay kinetics. This study provides infectivity-validated decay parameters for WSSV in seawater and sediments. These findings demonstrate that sediment serves as a long-term reservoir of WSSV infectivity, whereas seawater functions as a rapid transmission pathway, providing a quantitative basis for reservoir-mediated transmission risk assessment and biosecurity.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"615 ","pages":"Article 743658"},"PeriodicalIF":3.9,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}