Aquaculture最新文献_第4页

High breeding potential for growth exists in normal, black, and golden shell lines of tetraploid Fujian oyster revealed through three generations of mass selection 通过三代质量选择，发现福建四倍体牡蛎正常壳系、黑色壳系和金色壳系具有较高的繁殖潜力

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-26 DOI: 10.1016/j.aquaculture.2025.743595

Yuanxin Liang , Qi Li , Xianchao Bai , Geng Cheng , Jianmin Zhou , Rui Xu , Fanzhi Chu , Zhiyang Cui , Chengxun Xu , Wenshu Luo

The essential traits (such as anti-stress resistance, shell color, etc.) from tetraploid oysters could be inherited in triploids. Currently, the limited availability of high-quality tetraploid Fujian oysters (Crassostrea angulata) has failed to satisfy the polymorphic demands for the triploids in the market, particularly those with distinctive colors. We have successfully developed the normal (TNN), black (TBB) and golden (TGG) shell line of tetraploid C. angulata. However, the effectiveness of genetic improvement in these three lines remains unknown, which seriously hinders the development and adjustment of breeding programs for these three tetraploid lines of C. angulata. In this study, three consecutive generations of mass selection targeting shell height were conducted from 2022 to 2024 in these three lines of tetraploid C. angulata. The shell height (SH) and living weight (LW) were significantly higher in the selected groups of all three lines compared to the control group across all three generations. The realized heritability (

h_{R}^{2}

) for SH was 0.44, 0.47 and 0.42 for TNN, TBB and TGG, respectively. Across three consecutive generations, the average genetic gain in SH achieved by the three lines was 17.26 %. Furthermore, the living weight, as an indirectly selected trait, demonstrated genetic gains of 21.66 %, 24.00 %, and 18.97 % in the three lines, with an overall mean improvement of 21.54 %. In addition, the tetraploid rates reached 98.33 %, 99.00 %, and 96.67 % for the three lines, respectively, in the third generation. No significant variation in the median lethal temperature (LT₅₀) was observed within each of the three lines across the three consecutive generations. This study demonstrated that substantial additive genetic variation remains in the three tetraploid lines of C. angulata, indicating the potential for genetic improvement through selective breeding. The three improved tetraploid lines have enriched the germplasm of tetraploid oysters and provided valuable broodstock for the breeding of high-quality triploids with distinctive shell colors.

四倍体牡蛎的基本性状（如抗逆性、壳色等）可以在三倍体中遗传。目前，优质四倍体福建牡蛎（Crassostrea angulata）的供应有限，无法满足市场对三倍体，特别是具有独特颜色的三倍体的多态性需求。我们成功地培育了四倍体角田鸡正常壳系（TNN）、黑色壳系（TBB）和金色壳系（TGG）。然而，这3个品系的遗传改良效果尚不清楚，这严重阻碍了这3个四倍体品系育种计划的制定和调整。本研究从2022年到2024年，对这3个四倍体角田青（C. angulata）品系进行了连续3代以壳高为目标的群体选择。3个品系的壳高（SH）和活重（LW）在3代间均显著高于对照组。TNN、TBB和TGG对SH的实现遗传力（hR2）分别为0.44、0.47和0.42。在连续三代中，3个品系的平均遗传增益为17.26%。此外，作为间接选择性状的活重，3个品系的遗传增益分别为21.66%、24.00%和18.97%，总体平均提高21.54%。3个品系在第3代的四倍体率分别达到98.33%、99.00%和96.67%。3个品系的中位致死温度（LT50）在连续3代间均无显著差异。本研究表明，三种四倍体系存在大量的加性遗传变异，表明可以通过选择性育种进行遗传改良。这3个四倍体改良系丰富了四倍体牡蛎的种质资源，为培育具有独特壳色的优质三倍体提供了宝贵的亲本。

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引用次数: 0

Assessing the efficacy of a mycotoxin detoxifier in counteracting Aflatoxicosis in Pacific white shrimp Litopenaeus vannamei 评估一种霉菌毒素解毒剂对抗凡纳滨对虾黄曲霉中毒的效果

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-26 DOI: 10.1016/j.aquaculture.2025.743577

Eman Zahran , Gehad E. Elshopakey , Samia Elbahnaswy , Shaaban M. Abdel-Fattah , Ahmed I.A. Mansour , Shaymaa Rezk , Walaa El-Houseiny , Noha I. ElBanna , Dina Ibrahim , Óscar Castro , Eva León , Rokia Temmar , Raquel Codina , Insaf Riahi , Amany M. Shosha

This study investigated the effects of aflatoxin B1 (AFB1) dietary contamination and mycotoxin detoxifier (MD) on growth performance, AFB1 residues, immune response, and histomorphometry in Pacific white shrimp (Litopenaeus vannamei). Shrimp were fed diets containing AFB1 (50 μg/kg), a mycotoxin detoxifier, or a combination of both, for 8 weeks. AFB1 exposure considerably decreased growth performance, total hemocyte count (THC), digestive enzyme activities, and antioxidant capacity, and concurrently elevated oxidative stress markers and liver enzyme activities. Furthermore, it inhibits immune-related gene expression and induces histological damage in the intestines and hepatopancreas. Supplementation with mycotoxin detoxifiers mitigated most of the toxic effects of AFB1, enhancing growth, immune response, antioxidant status, and tissue morphology. The mycotoxin detoxifier effectively decreased AFB1 residues in shrimp muscles. These findings highlight the toxic effect of AFB1 on the L. vannamei and the mycotoxin detoxifier as a prospective strategy to antagonize aflatoxicosis in shrimp cultivation.

本试验研究了饲料中黄曲霉毒素B1 （AFB1）污染和霉菌毒素解毒剂（MD）对凡纳滨对虾（Litopenaeus vannamei）生长性能、AFB1残留量、免疫反应和组织形态学的影响。分别饲喂含有AFB1 （50 μg/kg）、霉菌毒素解毒剂或两者同时添加的饲料8周。AFB1暴露显著降低了生长性能、总血细胞计数（THC）、消化酶活性和抗氧化能力，同时提高了氧化应激标志物和肝酶活性。此外，它还能抑制免疫相关基因的表达，诱导肠和肝胰腺的组织学损伤。补充真菌毒素解毒剂减轻了AFB1的大部分毒性作用，促进了生长、免疫反应、抗氧化状态和组织形态。真菌毒素解毒剂可有效降低虾肌中AFB1残留。这些研究结果表明，AFB1对凡纳梅和霉菌毒素解毒剂的毒性作用是对虾养殖中拮抗黄曲霉中毒的一种有前景的策略。

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引用次数: 0

Selection for a single nucleotide polymorphism on chromosome 8 of the genome of Crassostrea gigas improves resistance to both ostreid herpesvirus 1 and Vibrio coralliilyticus 长牡蛎基因组第8染色体单核苷酸多态性的选择提高了对卵巢疱疹病毒1和珊瑚弧菌的抗性

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-26 DOI: 10.1016/j.aquaculture.2025.743597

Spencer L. Lunda , Konstantin Divilov , Timothy J. Green , Ben J.G. Sutherland , Xisheng Wang , Ling Jin , Jeremy Jennings , Mostafa Mandour , Ryan S. Mueller , Chris Langdon

Selection for a single nucleotide polymorphism (SNP) on chromosome 8 improved the survival of oyster spat in Tomales Bay, California, where ostreid herpesvirus 1 (OsHV-1) is endemic. To evaluate whether this improved survival was due to increased resistance to OsHV-1 and to determine whether selection for this SNP affects the resistance of larvae and spat to other pathogens, 12 crosses of C. gigas were produced with offspring that were either heterozygous for the SNP or homozygous for the reference allele (control families). Larvae were challenged with Vibrio coralliilyticus RE22 and OsHV-1 (Tomales variant). Larvae that were heterozygous for the putatively protective allele showed a 5.4 % increase in average survival compared to control families challenged with RE22 but no difference in survival against OsHV-1. Spat were challenged with RE22 and OsHV-1 (United Kingdom μvar). Spat that were heterozygous for the SNP showed a 28.1 % increase in average survival against RE22, and 38.9 % greater survival against OsHV-1. Degree of inbreeding was negatively associated with survival in both spat laboratory challenges. Spat were also planted in Tomales Bay, and the growth, survival, and prevalence of OsHV-1 infection in the planted oysters were assessed. Outplanted oysters that were heterozygous for the SNP had a 10.4 % increase in average survival than control families, lower prevalence of infection with OsHV-1, and lower viral loads in infected oysters. Additional spat were planted in Willapa Bay, Washington, and survival and growth were equivalent between families of the two genotypes. Across all families, survival in Tomales Bay was significantly correlated with survival after spat laboratory challenges with V. coralliilyticus (r = 0.60) and OsHV-1 (r = 0.59). These results confirm that the heterozygous genotype confers resistance of spat to OsHV-1 in laboratory and field trials and to a bacterial pathogen of oysters.

选择8号染色体上的单核苷酸多态性（SNP）提高了加州Tomales Bay牡蛎的存活率，那里是ostreid herpesvirus 1 （OsHV-1）的地方性地区。为了评估这种存活率的提高是否由于对OsHV-1的抗性增强，并确定这种SNP的选择是否会影响幼虫和幼虫对其他病原体的抗性，我们培育了12个gigas的杂交，其后代要么是SNP的杂合，要么是参考等位基因的纯合（对照家族）。用珊瑚弧菌RE22和OsHV-1（雄性变异）攻毒幼虫。与RE22攻毒的对照家族相比，对该假定的保护性等位基因杂合的幼虫的平均存活率增加了5.4%，但对OsHV-1的存活率没有差异。用RE22和OsHV-1 （uk μvar）攻毒。结果表明，SNP杂合子对RE22的平均存活率提高28.1%，对OsHV-1的平均存活率提高38.9%。在这两种实验室挑战中，近亲繁殖的程度与存活率呈负相关。在Tomales湾种植牡蛎，并对养殖牡蛎的生长、存活和OsHV-1感染情况进行评估。SNP杂合的外植牡蛎的平均存活率比对照家庭高10.4%，感染OsHV-1的流行率较低，感染牡蛎的病毒载量较低。在华盛顿州的威拉帕湾种植了更多的贝，两种基因型的家庭之间的生存和生长是相同的。在所有家庭中，番茄湾的存活率与被珊瑚弧菌（r = 0.60）和OsHV-1攻击后的存活率显著相关（r = 0.59）。这些结果证实，在实验室和田间试验中，杂合基因型赋予贝对OsHV-1和牡蛎细菌病原体的抗性。

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引用次数: 0

Impaired Sertoli-Spermatogonia interactions contribute to oligospermia and infertility in F1 captive-bred male Solea senegalensis 精子-精子细胞相互作用受损导致F1人工繁殖的塞内加尔独角鲸雄性少精子症和不育

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-25 DOI: 10.1016/j.aquaculture.2025.743593

Guillermo Barturen , Diego Robledo , Francisca Robles , Rose Ruiz Daniels , Maialen Carballeda , Dorinda Torres-Sabino , Rafael Navajas-Pérez , Paulino Martínez , Carmelo Ruiz-Rejón , Roberto De la Herrán

Reproductive dysfunction of captive-bred males of the Senegalese sole (Solea senegalensis) represents a significant bottleneck for its aquaculture, as these fish exhibit reduced sperm production and impaired fertility compared to wild-bred counterparts acclimated to farm conditions. To elucidate the cellular and molecular mechanisms underlying this phenomenon, single-nuclei RNA sequencing was performed on gonadal tissue from adult captive-bred and wild-bred males. The analysis yielded a high-quality dataset comprising ∼80.000 cells, which were grouped into eleven distinct clusters representing all major germline and somatic cell types, including spermatogonial stem cells, differentiating spermatogonia, spermatocytes, spermatids, Sertoli cells, Leydig cells, immune cells, and peritubular myoid cells. It is noteworthy that captive-bred males exhibited a marked overrepresentation of proliferative spermatogonia and a significant reduction in mature spermatids, suggesting a disruption in the progression of spermatogenesis. Differential expression and functional enrichment analyses revealed that spermatogonial cells in captive-bred males displayed heightened translational activity alongside downregulation of pathways related to cell-cell communication and interaction. Focused in-silico cell-cell communication analyses further suggested potential defective Sertoli-spermatogonia interactions as a key factor contributing to oligospermia and infertility of captive-bred males. This study provides the first single-nuclei transcriptomic atlas of the Senegalese sole male gonad, offering valuable insights into the molecular basis of reproductive failure in captivity related to gonadal development. The findings of the study will inform future strategies to enhance selective breeding and improve aquaculture productivity for this economically important species.

人工繁殖的塞内加尔比目鱼（Solea senegalensis）雄性生殖功能障碍是其水产养殖的一个重大瓶颈，因为与适应农场条件的野生比目鱼相比，这些鱼表现出精子产量减少和生育能力受损。为了阐明这一现象背后的细胞和分子机制，研究人员对成年圈养雄性和野生雄性的性腺组织进行了单核RNA测序。该分析产生了一个高质量的数据集，包括约80,000个细胞，这些细胞被分为11个不同的集群，代表所有主要的种系细胞和体细胞类型，包括精原细胞、分化精原细胞、精母细胞、精母细胞、支持细胞、间质细胞、免疫细胞和管周肌样细胞。值得注意的是，圈养繁殖的雄性表现出增殖性精原细胞的显著过度代表和成熟精子的显著减少，这表明精子发生的进程受到破坏。差异表达和功能富集分析显示，人工饲养的雄性精原细胞的翻译活性升高，同时细胞间通信和相互作用相关通路下调。集中的硅细胞-细胞通讯分析进一步表明，潜在的有缺陷的支持细胞-精原细胞相互作用是导致人工繁殖雄性少精子症和不育的关键因素。这项研究提供了塞内加尔唯一雄性性腺的第一个单核转录组图谱，为与性腺发育相关的圈养繁殖失败的分子基础提供了有价值的见解。这项研究的结果将为今后加强这种经济上重要物种的选择性育种和提高水产养殖生产力提供信息。

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引用次数: 0

Identification and characterization of interleukin-11b in the defense against bacterial infection in Nile tilapia (Oreochromis niloticus) 尼罗罗非鱼（Oreochromis niloticus）白细胞介素-11b防御细菌感染的鉴定与鉴定

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-24 DOI: 10.1016/j.aquaculture.2025.743591

Yujie Wang , Huaqing Sun , Xiaonuan Li , Chaoli He , Yiwen Chai , Xintong Zheng , Lu Zhang , Tingting Weng , Meng Chen , Zheng Guo , Jianmin Ye , Bingxi Li

Interleukin-11 (IL-11) acts as a crucial cytokine participating in immunoregulation and the activation of immune cells, including lymphocytes. In this research, we cloned and characterized the interleukin-11b molecule from Nile tilapia (Oreochromis niloticus), termed OnIL-11b, and investigated its functional role in anti-bacterial defense. A 200-residue polypeptide is encoded by the 603-base pair open reading frame of OnIL-11b. Structural analysis revealed four conserved α-helices, resembling the topology of mammalian IL-11. Phylogenetically, OnIL-11b clustered most closely with IL-11b from Atlantic halibut (Hippoglossus hippoglossus). The IL-11b gene is widely expressed in the tissues of healthy tilapia, with the highest expression level observed in the intestine, followed closely by several immune-related tissues, including peripheral blood, spleen, and head kidney. Following challenges with Streptococcus agalactiae (S. agalactiae), Aeromonas hydrophila (A. hydrophila) and lipo-poly saccharide (LPS), the mRNA expression of OnIL-11b was significantly up-regulated in peripheral blood, spleen, and head kidney, both in vivo and in vitro. Additionally, the levels of OnIL-11b protein in the spleen tissues of tilapia and in the supernatant of splenic lymphocytes in vitro were significantly increased following challenges with S. agalactiae. Functionally, recombinant OnIL-11b protein ((r)OnIL-11b) significantly upregulated the expression of inflammatory factors in the head kidney and spleen of tilapia during in vivo S. agalactiae infection, and analogous pro-inflammatory activity was detected in vitro after lymphocyte co-culture. Conversely, OnIL-11b knockdown elicited opposing in vivo effects: it significantly reduced pro-inflammatory factor expression while elevating tilapia bacterial load and mortality post S. agalactiae challenge. In conclusion, this study elucidates the characteristics of OnIL-11b and its pivotal role in antibacterial responses.

白细胞介素-11 （IL-11）是一种重要的细胞因子，参与免疫调节和免疫细胞（包括淋巴细胞）的激活。本研究从尼罗罗非鱼（Oreochromis niloticus）中克隆并鉴定了白细胞介素11b分子，命名为OnIL-11b，并研究了其在抗菌防御中的功能。OnIL-11b的603碱基对开放阅读框编码了200个残基的多肽。结构分析显示了4个保守的α-螺旋，类似于哺乳动物IL-11的拓扑结构。在系统发育上，来自大西洋大比目鱼（Hippoglossus Hippoglossus）的IL-11b与IL-11b聚类最接近。IL-11b基因在健康罗非鱼的组织中广泛表达，在肠道中表达量最高，其次是外周血、脾、头肾等免疫相关组织。在无乳链球菌（S. agalactiae）、嗜水气单胞菌（A. hydroophila）和脂多糖（LPS）的刺激下，OnIL-11b mRNA在体内和体外的外周血、脾脏和头肾中的表达均显著上调。此外，罗非鱼脾脏组织和脾脏淋巴细胞上清液中OnIL-11b蛋白水平在体外培养过程中显著升高。在功能上，重组OnIL-11b蛋白（(r)OnIL-11b）在体内无乳链球菌感染时显著上调罗非鱼头肾和脾脏中炎症因子的表达，并且在体外淋巴细胞共培养后检测到类似的促炎活性。相反，敲低OnIL-11b会引起相反的体内效应：它显著降低促炎因子的表达，同时提高罗非鱼的细菌负荷和无乳链球菌攻击后的死亡率。总之，本研究阐明了OnIL-11b的特性及其在抗菌反应中的关键作用。

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引用次数: 0

Assessment of the efficacy of percussive stunning in the industrial slaughter of turbot: New evidence from electroencephalography 工业屠宰大比目鱼冲击击晕效果评估：来自脑电图的新证据

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-24 DOI: 10.1016/j.aquaculture.2025.743590

C.A. Ramirez-Rodriguez , A. Verde , M.A. López-Patiño , M. Conde-Sieira , M. Alborja-Valado , O. González -Barreiro , A. Riaza , M. Chivite , J.M. Míguez

The current welfare guidelines for slaughtering fish state that individuals should be rendered unconscious during the process using appropriate stunning methods. The most common method of slaughtering turbot is live chilling, but this method has been questioned as it does not render the fish unconscious immediately and could therefore subject them to stressful conditions.

Consequently, farmers have turned their attention to finding alternative stunning methods for this species, such as electronarcosis or percussive stunning. However, their effectiveness under industrial conditions still needs to be proven. Our study aimed to evaluate the efficacy of percussive stunning in rendering turbot unconscious using electroencephalographic (EEG) and visual evoked response (VER) analysis together with traditional visual sensory indicators. The results showed that percussion causes a very rapid decrease in brain electrical activity, particularly affecting the high-frequency alpha and beta waves associated with conscious activity. Additionally, the absence of VERs was observed 15 min following contusion, confirming significant inhibition of brain function and sensory perception. Limitations in EEG recording caused by the percussion procedure meant that data could not be obtained for the first two minutes after stunning. However, the absence of fish responses to sensory stimuli allowed us to rule out signs of consciousness. Furthermore, when turbot were sacrificed using percussive stunning and immediate gill cutting, there was a complete lack of sensory responses and a sharp drop in electroencephalographic activity, consistent with death. Overall, these results support percussive stunning as a humane method of slaughter in the turbot industry.

目前关于屠宰鱼类的福利准则规定，在屠宰过程中，应该使用适当的电击方法使个体失去知觉。屠宰大比目鱼最常见的方法是活体冷却，但这种方法受到质疑，因为它不会使鱼立即失去知觉，因此可能使它们处于压力状态。因此，农民已将注意力转向为该物种寻找其他击晕方法，如电晕或击晕。然而，它们在工业条件下的有效性仍有待证实。采用脑电图（EEG）和视觉诱发反应（VER）分析，结合传统的视觉感觉指标，评价冲击休克对大比目鱼昏迷的疗效。结果表明，敲击会导致脑电活动的迅速减少，尤其是对与有意识活动相关的高频α和β波的影响。此外，挫伤后15分钟观察到ver的缺失，证实了脑功能和感觉知觉的显著抑制。脑电图记录的局限性是由敲击程序引起的，这意味着在昏迷后的前两分钟无法获得数据。然而，鱼类对感官刺激的反应的缺失使我们能够排除意识的迹象。此外，当使用冲击击晕和立即切鳃法处死大比目鱼时，感觉反应完全丧失，脑电图活动急剧下降，与死亡一致。总的来说，这些结果支持在大比目鱼行业中，撞击击晕是一种人道的屠宰方法。

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引用次数: 0

Corrigendum to ‘Gallic acid acts as an anti-inflammatory agent via PPARγ-mediated immunomodulation and antioxidation in fish gut-liver axis’ [Aquaculture 578 (2024) 740142] “没食子酸通过ppar γ介导的免疫调节和抗氧化在鱼肠-肝轴中作为抗炎剂”[水产养殖578(2024)740142]的勘误表

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-24 DOI: 10.1016/j.aquaculture.2025.743567

Xuyang Zhao , Lei Zhang , Nan Wu , Yuhang Liu , Jiayuan Xie , Lian Su , Qingsong Zhu , Bruno Hamish Unger , Fatima Altaf , Yuhang Hu , Weidong Ye , Zhixian Qiao , Yingyin Cheng , Wanting Zhang , Yaping Wang , Xiao-Qin Xia

引用次数: 0

Anesthetic potential of Cymbopogon citratus essential oil in tambaqui juveniles Colossoma macropomum 香蒲精油对大巨象幼鱼的麻醉作用

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-24 DOI: 10.1016/j.aquaculture.2025.743588

Jonas da Silva Costa , Lenise Vargas Flores da Silva , Hugo Napoleão Pereira da Silva , Luciano Jensen Vaz , Michelle Midori Sena Fugimura , Laura Giovanna Carvalho , Rosineia de Oliveira Silva , João Paulo Viana Figueira , Paula Raniele Freitas Tavares , Rosa Helena Veras Mourão , Matheus Dellaméa Baldissera , Bernardo Baldisserotto , Lilian Dena dos Santos

The aim of this study was to evaluate different concentrations of Cymbopogon citratus essential oil (EOCC) for induction and anesthetic recovery in tambaqui (Colossoma macropomum) and to verify its effects on biochemical parameters and oxidative stress. Two experiments were conducted, as follow: in experiment 1, the fish (N = 40, 10.06 ± 0.31 cm, and 9.9 ± 0.38 g) were exposed to different concentrations of EOCC (60, 80, 100, 120 mg/L) or an alcohol as control. In experiment 2, juveniles were submitted to biometry under deep anesthesia at the best concentration obtained in experiment 1 (100 mg/L EOCC – N = 24) and the water control group (N = 24). After 0, 30, 60, and 120 min of recovery, euthanasia was performed, and tissue samples were collected for biochemical and oxidative stress analyses. In experiment 1, it was observed that EOCC concentrations of 100 and 120 mg/L resulted in a significantly faster induction of anesthesia compared to lower concentrations. In experiment 2, EOCC stimulated the antioxidant activity of substances such as superoxide dismutase (SOD) and reduced glutathione (GSH) in tissues, with lower levels of thiobarbituric acid reactive substances (TBARS). Therefore, the use of EOCC at a concentration of 100 mg/L is recommended to anesthetize tambaqui juveniles in biometric management.

本研究旨在评价不同浓度香茅精油（Cymbopogon citratus精油，EOCC）诱导和麻醉恢复对巨像（Colossoma macropomum）的作用，并验证其对生物化学参数和氧化应激的影响。实验1分别将N = 40、10.06±0.31 cm和9.9±0.38 g的鱼暴露于不同浓度的EOCC（60、80、100、120 mg/L）和酒精中作为对照。在实验2中，以实验1中获得的最佳浓度（100 mg/L EOCC - N = 24）和水对照组（N = 24）在深度麻醉下进行生物测量。恢复0、30、60和120分钟后，进行安乐死，并收集组织样本进行生化和氧化应激分析。在实验1中，我们观察到EOCC浓度为100和120 mg/L时的麻醉诱导速度明显快于较低浓度。在实验2中，EOCC刺激了组织中超氧化物歧化酶（SOD）和还原性谷胱甘肽（GSH）等物质的抗氧化活性，并降低了硫代巴比妥酸活性物质（TBARS）的水平。因此，在生物识别管理中，推荐使用浓度为100 mg/L的EOCC麻醉坦巴基幼鱼。

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引用次数: 0

Recent understanding of crosstalk between muscle and different organs in regulating freshwater fish flesh quality under stress condition: A review 应激条件下淡水鱼肉质调控中肌肉与各器官间的串扰研究进展

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-24 DOI: 10.1016/j.aquaculture.2025.743574

Liwen Wu , Liangzi Zhang , Ling Peng , Yong Long , Guili Song , Soottawat Benjakul , Juan You , Ru Liu , Qilin Huang , Huawei Ma , Dan Jia , Tao Yin

Fish flesh, as the primary protein source in human diets, derives economic value from quality attributes. Fish is frequently stressed during the whole industry chain including aquaculture, transport, and slaughter, which significantly influences fish flesh quality. Multicellular organisms maintain body homeostasis through the division of labor and cooperation among their various organ and tissue systems. Skeletal muscle functions not only as an effector organ within the motor system but also as a crucial regulator of metabolism. Under stress conditions, skeletal muscle enhances its functions across multiple dimensions to achieve adaptive responses. This adaptation necessitates the formation of a crosstalk or muscle-organ axis reaction between muscle and organs such as brain, gut, and liver, ultimately altering flesh quality. In recent years, the effect of the crosstalk between muscle and different organs in regulating flesh quality has progressively become a frontier hot spot. However, the information available is largely scattered and disjointed. Here, we systematically review the crosstalk mechanism between muscle and different organs under stress conditions according to the classification of key signaling pathways or molecules. Furthermore the effects of crosstalk on fish flesh quality are summarized, including nutrition, pHysical properties, and flavor. This review provides new perspectives and potential strategies for food processing and biotechnology in fish flesh quality control.

鱼肉作为人类饮食中主要的蛋白质来源，其品质属性具有经济价值。鱼类在养殖、运输、屠宰等全产业链中受到的压力较大，对鱼肉品质影响较大。多细胞生物通过其各个器官和组织系统之间的分工和合作来维持体内稳态。骨骼肌不仅是运动系统中的效应器，而且是代谢的重要调节器官。在应激条件下，骨骼肌在多个维度上增强其功能，以实现适应性反应。这种适应需要在肌肉和大脑、肠道和肝脏等器官之间形成一种串音或肌肉-器官轴反应，最终改变肉质。近年来，肌肉与不同器官之间的相互作用对肉质调节的作用逐渐成为一个前沿热点。然而，可获得的信息在很大程度上是分散和脱节的。在此，我们根据关键信号通路或分子的分类，系统地综述了应激条件下肌肉与不同器官之间的串扰机制。并从营养、物理和风味等方面综述了相声对鱼肉品质的影响。本文为食品加工和生物技术在鱼肉质量控制中的应用提供了新的前景和可能的策略。

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引用次数: 0

Dual roles of host proteins in viral defense and evasion: Implications for selective breeding and disease management in groupers 宿主蛋白在病毒防御和逃避中的双重作用：对石斑鱼的选择育种和疾病管理的启示

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-23 DOI: 10.1016/j.aquaculture.2025.743586

KhaiHang Choong , ZeXin Li , LeongSeng Lim , Annita Seok Kian Yong , Peng Xu , Karsoon Tan

Grouper aquaculture, a vital part of global marine fisheries, is facing increasing threats from infectious diseases like Singapore grouper iridovirus (SGIV), Red-spotted nervous necrosis virus (RGNNV), and Vibrio spp. These diseases cause systemic infections, high mortality, and economic losses. This scoping review synthesizes advances in selective breeding for pathogen resistance in grouper, leveraging genomic tools and molecular insights to inform sustainable aquaculture strategies. 230 studies were analyzed, revealing the dual roles of host proteins in viral defense and evasion. This report synthesizes available molecular-breeding literature to enumerate the demonstrable advantages of molecular-based selection against disease threat in grouper. TRIM proteins inhibit SGIV/RGNNV replication via interferon signalling, while TRIM62 and TRIM35 enhance viral proliferation by suppressing NF-κB and MAVS pathways. Autophagy-related proteins promote SGIV replication by exploiting autophagosome formation, while heat shock proteins modulate immune responses. Non-coding RNAs regulate viral replication by dampening interferon responses, while circular RNAs and lncRNAs modulate immune gene networks during infection. Genomic technologies identified 54 SNPs linked to RGNNV resistance in Epinephelus lanceolatus, while QTL mapping in hybrid grouper uncovered 39 loci associated with growth and immune traits. CRISPR/CasRx demonstrated efficacy in reducing RGNNV loads via intracranial delivery, while DNA aptamers enabled rapid, cost-effective diagnostics for SGIV. Challenges persist, including pathogen immune evasion and technological gaps in high-throughput genotyping. Future directions emphasise multi-omics integration to decode immune-metabolic crosstalk, while proteomic profiling of mucosal exosomes and machine learning-driven CRISPR design offer non-invasive biomarkers and precision gene editing. By prioritizing multi-trait selection and translational research, stakeholders can mitigate disease impacts while enhancing resilience to climate and microbial threats, ensuring the long-term viability of this vital industry.

石斑鱼养殖业作为全球海洋渔业的重要组成部分，正面临着新加坡石斑鱼虹膜病毒（SGIV）、红斑神经坏死病毒（RGNNV）和弧菌等传染病日益严重的威胁，这些疾病造成全身感染、高死亡率和经济损失。本综述综合了石斑鱼病原体抗性选择性育种的进展，利用基因组工具和分子见解为可持续水产养殖战略提供信息。分析了230项研究，揭示了宿主蛋白在病毒防御和逃避中的双重作用。本报告综合了现有的分子育种文献，列举了基于分子的选择对抗石斑鱼疾病威胁的明显优势。TRIM蛋白通过干扰素信号抑制SGIV/RGNNV复制，而TRIM62和TRIM35通过抑制NF-κB和MAVS途径增强病毒增殖。自噬相关蛋白通过利用自噬体的形成促进SGIV复制，而热休克蛋白则调节免疫反应。非编码rna通过抑制干扰素反应来调节病毒复制，而环状rna和lncRNAs在感染过程中调节免疫基因网络。基因组技术鉴定出54个snp与石斑鱼（Epinephelus lanceolatus）的RGNNV抗性相关，而杂交石斑鱼的QTL定位发现了39个与生长和免疫性状相关的位点。CRISPR/CasRx证明了通过颅内递送减少RGNNV负荷的有效性，而DNA适体则能够快速、经济地诊断SGIV。挑战依然存在，包括病原体免疫逃避和高通量基因分型方面的技术差距。未来的方向强调多组学整合来解码免疫代谢串扰，而粘膜外泌体的蛋白质组学分析和机器学习驱动的CRISPR设计提供非侵入性生物标志物和精确的基因编辑。通过优先考虑多性状选择和转化研究，利益相关者可以减轻疾病影响，同时增强对气候和微生物威胁的抵御能力，确保这一重要行业的长期生存能力。

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