Aquaculture最新文献

{"title":"Development of a duplex fluorescent in situ hybridization (FISH) technique to detect and localize Redspotted Grouper Nervous Necrosis Virus sense and antisense genomic RNA1 and its application to the tissues of aquatic vertebrates and invertebrates","authors":"Francesca Errani ,&nbsp;Enrico Volpe ,&nbsp;Madeleine de le Roi ,&nbsp;Christina Puff ,&nbsp;Luciana Mandrioli ,&nbsp;Vito Antonio Baldassarro ,&nbsp;Wolfgang Baumgärtner ,&nbsp;Sara Ciulli","doi":"10.1016/j.aquaculture.2025.742249","DOIUrl":"10.1016/j.aquaculture.2025.742249","url":null,"abstract":"<div><div>The causative agent of viral encephalopathy and retinopathy (VER), the nervous necrosis virus (NNV), is considered one of the most impacting agents in the Mediterranean aquaculture and it has already been described in several aquatic vertebrates and invertebrates including bivalve molluscs.</div><div>In this study a novel duplex fluorescent <em>in situ</em> hybridization (FISH) assay was developed for the detection of sense and antisense RGNNV genomic RNA1 fragment, coding for the RNA-dependent RNA polymerase (RdRp), and applied to aquatic vertebrates and invertebrates' tissues. The detection of sense and antisense RNA provides useful information not only on viral localization at cellular level, but also on viral replication, improving the understanding of the host-pathogen interactions between NNV and its hosts.</div><div>The designed assay resulted in the specific detection of the RdRp of the two NNV strains most widespread in the Mediterranean basin: RGNNV and RGNNV/SJNNV.</div><div>FISH was applied to the brain of European sea bass (<em>Dicentrarchus labrax</em>) showing the localization at cellular level of both NNV genome and cRNA produced during viral replication.</div><div>The assay was also able to localize the NNV genome and its replicative intermediate form in clams (<em>Ruditapes philippinarum</em>). Clear positive signals were found in oocytes' cytoplasm confirming the intracellular localization of NNV in invertebrates. Altogether, these findings suggest NNV active replication status in clams' oocytes and its potential transmission through vertical route. In conclusion, the developed assay can find wide application in the deep understanding of host-pathogen interactions, contributing to pointing out the real role that invertebrates play in VER epidemiology.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742249"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143360498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity and dietary manipulation can ameliorate extreme summer heatwave stress in European seabass, Dicentrarchus labrax","authors":"Md Jakiul Islam ,&nbsp;Amir Hossain ,&nbsp;Andreas Kunzmann","doi":"10.1016/j.aquaculture.2025.742252","DOIUrl":"10.1016/j.aquaculture.2025.742252","url":null,"abstract":"<div><div>Climate change-induced extreme heatwaves are rapidly becoming a common environmental stressor, having a significant impact on fish. There is a growing emphasis on researching mitigation options for fish to fare better during heatwave events. To explore potential mitigation strategies, European seabass, <em>Dicentrarchus labrax</em> (36.19 ± 2.68 g) was raised under four experimental conditions: Control [30 practical salinity unit (PSU)], Treatment-1 (12 PSU), Treatment-2 (12 PSU while fed the diet supplemented with 4.5 % propolis), and Treatment-3 (30 PSU while fed the diet supplemented with propolis) for 60 days followed by 20 days of heatwave (30 °C) exposure under controlled laboratory conditions. To understand fish response, this study focused on the assessment of growth, feeding performances, and a range of biochemical, metabolic, and molecular parameters. These findings indicate that fish reared in 12 PSU while fed propolis supplemented diet (Treatment-2) exhibited significantly higher final body weight (FWG), percent weight gain (PWG), and specific growth rate (SGR) compared to the control group (<em>p</em> &lt; 0.05). During heatwave exposure, fish in the 12 PSU group, whether fed propolis (Treatment-2) or not (Treatment-1), exhibited lower cortisol levels than the fish in control condition. On the 20th day of heatwave exposure, compared to fish in control condition, fish in the 12 PSU group, whether fed propolis or not, exhibited significantly higher superoxide dismutase assay (SOD) activity in gill tissue, whereas a contrasting trend was observed in muscle tissue. Glutathione peroxidase (GPx) activity was found significantly lower in fish in the 12 PSU group, whether fed propolis or not. On day 20 of heatwave exposure, the heat shock protein 70 (HSP70) and immunoglobulin growth factor 1 (Igf1), fatty acid desaturase 2 (FADS2), and tumor necrosis alpha (TNF-1α) genes were significantly (<em>p</em> &lt; 0.05) upregulated in the liver tissue of fish raised at 30 PSU group when fed propolis (Treatment-3). Most of the tested parameters exhibited variable patterns across tissues suggesting that the fish stress response is influenced by both propolis supplemented diet and varying salinities during heatwave exposure. During the heatwave exposure European seabass reared in 12 PSU while feeding on propolis-supplemented diet or not, demonstrated higher growth and physiological responses than fish in control condition. Moreover, there were positive physiological benefits observed in fish raised in 30 PSU (Treatment-3), while fed propolis supplemented diet. The study proposes that adjusting salinity level and incorporating propolis into the diet can be effective strategies to mitigate the negative impacts of heatwave in European seabass.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742252"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential roles of LMNA in promoting longevity for larger Argopecten scallops","authors":"Yang Zhao ,&nbsp;Junhao Ning ,&nbsp;Quanchao Wang ,&nbsp;Guilong Liu ,&nbsp;Xin Xu ,&nbsp;Rongjie Chen ,&nbsp;Chunde Wang ,&nbsp;Xia Lu","doi":"10.1016/j.aquaculture.2025.742254","DOIUrl":"10.1016/j.aquaculture.2025.742254","url":null,"abstract":"<div><div>Bay scallops, <em>Argopecten irradians</em>, are a commercial bivalve species, but their small size, short longevity, and serious inbreeding depression make it difficult to further promote the farming of this species. Compared with bay scallops, some interspecific hybrids bay scallops and Peruvian scallops (<em>A. purpuratus</em>) have extraordinary growth advantages, such as longer longevity and greater sizes, which can be attributed to the longevity genes of Peruvian scallops (7–10 years). Lamin A/C (LMNA) is a nuclear scaffold protein that interacts with specific histone deacetylases (HDAC) to maintain their activity and regulate gene expression. <em>LMNA</em> mutations can lead to premature aging, and altered levels of <em>LMNA</em> are found in diverse diseases in most vertebrates; however, its roles in regulating senescence remains largely unknown in mollusk species. In this study, we cloned <em>LMNA</em> open reading frames (ORFs) from two closely correlated <em>Argopecten</em> scallops with different lifespans and explored their capacity to modulate longevity. One hundred and twenty-eight variations and one InDel of three nucleotides were discovered in <em>ApLMNA</em> and <em>AiLMNA</em> at the nucleotide sequence level, with 109 synonymous and 19 non-synonymous SNPs, leading to 16 amino acid variations and the absence of S16 in <em>AiLMNA</em>. Tissue distribution analysis revealed that these two genes exhibited different trends during the development and aging of the two scallops. A yeast two-hybrid assays revealed a stronger interaction between LMNA and SIRT1 in <em>A. purpuratus</em> than in <em>A. irradians</em>. RNA interference (RNAi) of <em>ApLMNA</em> at 6 h significantly upregulated the expression of its downstream gene, <em>SIRT1</em>; however, <em>SIRT1</em> was downregulated after RNAi of <em>AiLMNA</em>. The <em>SIRT1-</em>targeted genes <em>FoxO</em>, <em>Mn-SOD</em>, and <em>CAT</em> also showed different expression patterns. Transcriptome analysis revealed that the longevity pathways in Peruvian scallops were activated after RNAi. Overall, our study provides interesting avenues to understand the mechanism of longevity through <em>LMNA</em> genes and will help produce long-lived and large crossbred offsprings to maintain fisheries and aquaculture going.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742254"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143359836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic parameters and genotype by environment interaction for harvest traits of Malabar red snapper (Lutjanus malabaricus)","authors":"Bing Liang ,&nbsp;Dean R. Jerry ,&nbsp;Vu Nguyen ,&nbsp;Xueyan Shen ,&nbsp;Joyce Koh ,&nbsp;Celestine Terence ,&nbsp;Maria G. Nayfa ,&nbsp;Maura Carrai ,&nbsp;Purushothaman Kathiresan ,&nbsp;Rachel Jia Wen Ho ,&nbsp;Hazim Mohamed ,&nbsp;Saraphina Dianne Rwei Qing Tneo ,&nbsp;Grace Loo ,&nbsp;Shubha Vij ,&nbsp;Jose A. Domingos","doi":"10.1016/j.aquaculture.2025.742247","DOIUrl":"10.1016/j.aquaculture.2025.742247","url":null,"abstract":"<div><div>Malabar red snapper (<em>Lutjanus malabaricus</em>) is a tropical fish farmed in Singapore and Southeast Asia. Currently, eggs are produced in open net cages without controlled breeding, and no breeding program exists. The industry needs access to high-quality eggs from genetically improved stocks, highlighting the importance of developing a breeding program. However, fundamental genetic information, such as heritability and genetic correlations, is needed before implementing such a program. In this study, 2547 Malabar red snappers were genotyped using a custom Axiom 70k Red Snapper SNP array across three different rearing sites in Singapore. The body weight (BW), total length (TL), body depth (BD), Fulton's condition factor (K), body shape index (BSI) and skin redness (CIELAB *a values; Cl_a) of the fish were collected at harvest when fish were 18 months old. The mean ± standard deviation (SD) of all fish samples were 635.1 ± 222.7 g (BW), 327.0 ± 39.4 mm (TL), 115.2 ± 14.4 mm (BD), 1.76 ± 0.23 (K), 3.63 ± 0.24 (BSI) and 4.6 ± 2.9 (Cl_a). Heritabilities (<em>h</em>^<em>2</em>), genetic correlations (<em>r</em>_<em>g</em>) and genotype by environment interactions (GxE interaction) were estimated using BLUPF90 with an animal mixed model using the genomic relationship matrix (GRM). Heritabilities were observed to be moderate for BW (0.29 ± 0.03), TL (0.30 ± 0.03), BD (0.39 ± 0.05), K (0.21 ± 0.03) and BSI (0.21 ± 0.03), but low for Cl_a (0.04 ± 0.02). High genetic correlations were present among both growth (BW, TL and BD, <em>r</em>_<em>g</em> ≥ 0.90) and body-shape traits (K and BSI, <em>r</em>_<em>g</em> = 0.91), but lower for comparisons between growth (BW and BD) and body-shape traits (K and BSI) (0.21 ± 0.10 to 0.43 ± 0.09). These results indicate that both harvest growth traits and body shape of Malabar red snapper could be improved via selective breeding programs, although selection of fast growers might not maximize the genetic gain for K and BSI. In contrast, the low <em>h</em>^<em>2</em> of red colouration suggested that environmental factors (e.g. dietary carotenoids) rather than genetic effects may be primarily responsible for the phenotypic variation observed in skin redness. Moderate GxE interactions were observed for BW (0.45 ± 0.25 to 0.60 ± 0.27), TL (0.31 ± 0.24 to 0.57 ± 0.27), BD (0.40 ± 0.24), K (0.36 ± 0.25 to 0.73 ± 0.91) and BSI (0.73 ± 0.26) among the three rearing sites, suggesting that a single breeding program may not deliver equal genetic gains for all farms alike, and that genomic selection algorithms should be trained on the rearing site where animals are to be farmed. In conclusion, the present study provided valuable information for the design of future selective breeding programs for Malabar red snapper.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742247"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic effects of Aeromonas sobria infection on intestinal morphology, microbiota and goblet cells of rainbow trout (Oncorhynchus mykiss)","authors":"Li-Ping Liu ,&nbsp;Peng-Tian Kang ,&nbsp;Feng-Yuan Ding ,&nbsp;Dong-Hong Shao ,&nbsp;Bao-Shan Wang ,&nbsp;Juan Yang ,&nbsp;Wen-Hui Gao ,&nbsp;Wang-Dong Zhang ,&nbsp;Wen-Hui Wang ,&nbsp;Ji-Xing Liu","doi":"10.1016/j.aquaculture.2025.742238","DOIUrl":"10.1016/j.aquaculture.2025.742238","url":null,"abstract":"<div><div><em>Aeromonas sobria</em> is an opportunistic pathogen that caused gastroenteritis and septicemia in humans, animals and aquatic species. However, its effects on the intestinal mucosal barrier remains unclear. In this paper, the dynamic effects of <em>A.sobria</em> on intestinal morphology, microbiota and goblet cells of rainbow trout (48 fish, weighing 400 ± 50 g) were revealed by histomorphology, 16S rRNA sequencing, ELISA and Alcian blue-periodic acid sthiff (AB-PAS) staining after isolating <em>A.sobria</em> from diseased rainbow trout during outbreak. The results showed that the expression of penicillin and cephalosporin biosynthesis increased significantly at 12–72 h. The level of Lipopolysaccharide (LPS) in feces and serum increased significantly at 12–72 h and 24–72 h, respectively (<em>P</em> &lt; 0.05). In addition, <em>A.sobria</em> infection induced dysbiosis but never caused significant changes in the relative abundance of Proteobacteria. At 48 h, the richness and diversity of intestinal flora increased significantly (<em>P</em> &lt; 0.001), while the number of goblet cells decreased significantly (P &lt; 0.001). Intestinal villi underwent necrosis, shedding and cellular vacuolar degeneration, resulting in the most severe damage. At 9 d, there was no significant difference in richness, but the diversity was significantly reduced (<em>P</em> &lt; 0.05), indicating that the intestinal flora had recovered its balance but could not return the initial state. The pathological results showed that the intestinal villi became shorter and wider spaced, and the mucosal lamina propria and muscle layer became thinner. At 15 d, the diversity of intestinal flora was still decreasing. Our study indicated that the intestinal dysbiosis and LPS production caused by <em>A.sobria</em> infection may be related to the activation of penicillin and cephalosporin biosynthesis, independent of Proteobacteria. Furthermore, increased diversity of intestinal flora and decreased number of goblet cells were the characteristics of intestinal tissue damage, while sustained decreased diversity and weakened resilience of intestinal flora were the hallmarks of chronic intestinal disease. This will lay a foundation for the study of pathogenic mechanism of <em>A.sobria</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742238"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143360497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early developmental exposure to heat shock altered the response to handling stress but had limited impacts on the susceptibility to the bacterial pathogen Yersinia ruckeri in juvenile rainbow trout (Oncorhynchus mykiss)","authors":"Rob J. McFarling ,&nbsp;Mickael Teixeira Alves ,&nbsp;Ellen Blaker ,&nbsp;Lyndsay Christie ,&nbsp;Claire Joiner ,&nbsp;Ronny van Aerle ,&nbsp;Eduarda M. Santos ,&nbsp;Ioanna Katsiadaki","doi":"10.1016/j.aquaculture.2025.742244","DOIUrl":"10.1016/j.aquaculture.2025.742244","url":null,"abstract":"<div><div>Disease is a major limiting factor for aquaculture production, having significant implications for production as well as fish welfare, hence, novel, preferably drug-free approaches for preventing and alleviating these impacts are required. Exposure of fish to heat shock during early embryo development has the potential to alter responses to stress in later life. Due to crosstalk between thermal stress and immune signalling pathways and encouraged by the well-documented ability for elevated temperatures to influence immune function, we hypothesised that heat shock during early developmental life stages could influence responses and survival during infection. To test this hypothesis, rainbow trout embryos were heat shocked (18 °C for one hour, initiated at 19 h post fertilisation), whilst naïve controls were maintained at 10 °C. Following hatching and development, juvenile fish from both groups were challenged with the bacterial pathogen <em>Yersinia ruckeri,</em> or mock challenged (exposed to handling stress associated with the procedure) in parallel. No significant difference in survival following infection was observed between naïve and heat shocked fish (log-rank test, <em>p</em> = 0.7). Interrogation of hepatic transcription prior to infection revealed minimal differences between naïve and heat shocked fish. However, comparing the response of naïve and heat shocked fish to handling stress associated with the mock challenge revealed substantial differences in hepatic transcription. Enriched pathways including unfolded protein response, apoptosis, metabolism, oxidative stress and DNA damage, showed that the embryonic exposure to heat shock modified the response to subsequent handling stress. In contrast, naïve and heat shocked fish exposed to <em>Y. ruckeri</em> displayed minor differences in transcriptomic responses, and no alterations in pathogen-challenge survival.</div><div>Overall, our data demonstrate the potential for developmental heat shock exposure to condition the response of fish to subsequent stressors, particularly to handling stress. Further work is required to build on these findings and determine the potential for developmental heat shock as a management tool to alleviate the adverse effects of exposure to stressors in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742244"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TOGCN analysis offers new insights into the accelerated skeletal muscle growth of mandarin fish relative to big-eye mandarin fish","authors":"Xiaoli Yao ,&nbsp;Jinhua Gao ,&nbsp;Hossam I. Kadira ,&nbsp;Jia Zheng ,&nbsp;Xiaojun Yang ,&nbsp;Yan Zhao ,&nbsp;Shoujie Tang ,&nbsp;Jinliang Zhao","doi":"10.1016/j.aquaculture.2025.742236","DOIUrl":"10.1016/j.aquaculture.2025.742236","url":null,"abstract":"<div><div>Unlike mammals, fish grow skeletal muscle throughout their lives. Skeletal muscle growth includes hyperplasia, fusion, and protein precipitation. Mandarin fish (<em>Siniperca chuatsi</em>) and big-eye mandarin fish (<em>Siniperca kneri</em>) are two sister species with extremely similar morphology, while their skeletal muscle growth rates exhibit considerable divergence. Previous studies have revealed a significant level of genomic similarity between these two species. However, the onset of the growth advantage in mandarin fish, early skeletal muscle growth characteristics, and the distinct mechanisms compared to big-eye mandarin fish remain ambiguous. This study collected mandarin fish and big-eye mandarin fish at 10, 20, and 30 dph, and then conducted skeletal muscle phenotypes and TOGCN (Time-ordered Gene Coexpression Network) analysis. The results showed that the growth rate of mandarin fish was higher than that of big-eye mandarin fish since 20 dph. Mandarin fish showed the trend of <em>pax7+/myod-</em> at 10–20 dph and <em>pax7−/myod</em> <em>+</em> at 20–30 dph, while there was no significant difference in <em>pax7</em> in big-eye mandarin fish. The TOGCN analysis showed that the sc + sk0/sc-sk0 group enriched specific genes for satellite cell regulation. The Notch pathway genes showed an increase at 10–20 dph and a decrease at 20–30 dph. PPI analysis showed that <em>fosab</em>, <em>gata3</em>, <em>cebpb, and hand2</em> were the hub DEGs, which are associated with the specific regulation of satellite cells in mandarin fish. In conclusion, satellite cells' proliferation and differentiation capacity account for the disparity in skeletal muscle growth rates between mandarin fish and big-eye mandarin fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742236"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143359837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gibberellin induces rapid regeneration of juveniles from the Sargassum fusiforme holdfasts","authors":"Lin Luo ,&nbsp;Dongya Bao ,&nbsp;Congquan Gao ,&nbsp;Xingda Sheng ,&nbsp;Peng Jin ,&nbsp;Xu Gao ,&nbsp;Zengling Ma ,&nbsp;Binbin Chen ,&nbsp;Mingjiang Wu","doi":"10.1016/j.aquaculture.2025.742248","DOIUrl":"10.1016/j.aquaculture.2025.742248","url":null,"abstract":"<div><div>Sexual reproduction of economically valuable <em>Sargassum fusiforme</em> can lead to genetic drift of useful strains and reduce their economic benefits. The number of juveniles from <em>S. fusiforme</em> holdfasts in the natural state is very small. Gibberellin (GA) may play an important role in <em>S. fusiforme</em> holdfast regeneration. This study further explored the application of GA to promote the rapid juvenile regeneration from <em>S. fusiforme</em> holdfast and explored its mechanism. Exogenous addition of 1.5 mg⸱L⁻¹ GA significantly shortened the regeneration time of <em>S. fusiforme</em> holdfast and induced more juvenile regeneration. KEGG analysis showed that the differential genes induced by GA treatment were mainly enriched in the ribosomal pathway, carbon assimilation metabolism, and oxidative phosphorylation pathway. GA not only enhanced the photosynthetic capacity of holdfast, but also upregulated the Ca²⁺ signaling pathway by enhancing <em>CaM</em> gene expression, up-regulating the expressions of <em>Ran</em> gene associated with cell cycle regulation, <em>MDH</em>, aconite hydratase, <em>GAPDH</em>, <em>FBA</em>, and <em>G6PDH</em> associated with carbon assimilation metabolism. In addition, it also upregulated the expression of F-type H⁺-transport ATPase subunit a, promoting the accumulation of nutrients in holdfasts and the supply of energy required for the regeneration, thus promoting their growth. Thus, exogenous induction of holdfasts regeneration with 1.5 mg⸱L⁻¹ GA₃ can be used to solve the problem of the scarce number of juveniles from holdfasts and obtain more stable genetically dominant strains for more and faster regeneration of juveniles, to improve the actual production efficiency.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742248"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143338944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QTL identification for growth-related traits using a high-density bin-map in Pacific oyster (Crassostrea gigas)","authors":"Hang Yang ,&nbsp;Yong Chi ,&nbsp;Yaru Zhou ,&nbsp;Qi Li","doi":"10.1016/j.aquaculture.2025.742246","DOIUrl":"10.1016/j.aquaculture.2025.742246","url":null,"abstract":"<div><div>Growth is an important economic trait in animal genetics and breeding. The Pacific oyster (<em>Crassostrea gigas</em>) is the major cultured species of oyster in global aquaculture, yet the genetic underpinnings associated with growth have not been fully elucidated. In this study, a high-density genetic map was constructed using a F₂ progeny with different growth rates, and then QTL mapping for seven growth-related traits were performed. The map consisted of 2465 bin markers (461,572 SNP loci) spread across 10 linkage groups within a total genetic coverage of 1117.16 cM and an average density of 0.45 cM. Ten QTLs related to shell length, shell width, total weight, shell weight and soft tissue weight were identified to be distributed on three different linkage groups, and 101 candidate genes were annotated. We found that some candidate genes have been known to be linked to energy metabolism, biomineralization, lipid metabolism, cellular growth and so on. This linkage map and QTLs for important economic traits of <em>C. gigas</em> will be a powerful tool for both fine-scale QTL mapping and marker-assisted selection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742246"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143360495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of 5-Hydroxymethylfurfural in mitigating transportation stress of large yellow croaker (Larimichthys crocea)","authors":"Jichang Zheng,&nbsp;Yuan Feng,&nbsp;Yongjiang Xu","doi":"10.1016/j.aquaculture.2025.742235","DOIUrl":"10.1016/j.aquaculture.2025.742235","url":null,"abstract":"<div><div>The stress response induced by the transportation of fish from nearshore cages to aquaculture vessel is a great challenge in open ocean aquaculture. Therefore, the present study investigated the stress mitigatory potency of 5-Hydroxymethylfurfural (5-HMF) during transportation in large yellow croaker (<em>Larimichthys crocea</em>) based on the stress hormones, antioxidant capacity, gill morphology and skin transcriptome. The large yellow croaker (388 ± 58.79 g) were transported for about 2.5 h in rectangular canvas tanks treated with or without 5-HMF (0.2 mg/L). Blood, gill and skin were sampled before the transportation (control, C0), during transportation (treated with/without 5-HMF, EC/TC), upon arrival (treated with/without 5-HMF, E0/T0) as well as 12, 24 and 72 h after transportation (treated with 5-HMF, E12/E24/E72; treated without 5-HMF, T12/T24/T72). Results showed that the serum cortisol, epinephrine and platelet number were significantly elevated in both treatments during transportation and at arrival, while these stress-related parameters tended to be reduced in fish exposed to 5-HMF than those not. Apart from that, transportation also exerted negative effects on osmoregulation, characterized by significantly reduced activity of Na⁺/K⁺-ATPase and Ca²⁺/Mg²⁺-ATPase in gill during transportation and early recovery, which was ameliorated by the addition of 5-HMF. Furthermore, the differentially expressed genes in skin of transportation and recovery phase compared with that of pre-transportation were enriched in PI3K-AKT signaling pathway. As a crucial component of this signaling pathway, the <em>hsp90α</em> expression was notably elevated in fish receiving 5-HMF than those not during transportation and early recovery phase. Therefore, the addition of 5-HMF to water at 0.2 mg/L can alleviate the transportation stress and accelerate the recovery process of large yellow croaker, and this mitigatory potency might be mainly achieved by enhancing <em>hsp90α</em> expression to expedite the clearance of misfolded proteins induced by transportation stress.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742235"},"PeriodicalIF":3.9,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143338964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}