Pub Date : 2025-04-03DOI: 10.1016/j.aquaculture.2025.742456
Munusamy Ajithkumar , Marie Lillehammer , Marie-Agnés Travers , Elise Maurouard , Muhammad Luqman Aslam , Lionel Dégremont
{"title":"Corrigendum to “Genetic parameters for resistance to field mortality outbreaks and resistance to a pathogenic strain of Vibrio splendidus in Mytilus edulis, Mytilus galloprovincialis and natural hybrid” [Aquaculture, Volume 590]","authors":"Munusamy Ajithkumar , Marie Lillehammer , Marie-Agnés Travers , Elise Maurouard , Muhammad Luqman Aslam , Lionel Dégremont","doi":"10.1016/j.aquaculture.2025.742456","DOIUrl":"10.1016/j.aquaculture.2025.742456","url":null,"abstract":"","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742456"},"PeriodicalIF":3.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-02DOI: 10.1016/j.aquaculture.2025.742513
Yundong Li , Jing An , Shigui Jiang , Song Jiang , JianHua Huang , LiShi Yang , Qibin Yang , Jianzhi Shi , Zhenhua Ma , Falin Zhou
The ovary maturation process poses a significant constraint on the commercial production and breeding of Penaeus monodon, one of the most extensively cultivated crustacean species. The reproductive stage is controlled by neuropeptide hormones and various proteins released from secretory sites (X-organ/sinus gland, XO/SG) within the eyestalk. Unilateral eyestalk ablation has been a widely employed method to artificially induce ovarian maturation in farmed P. monodon. To better understand the reproductive regulation mechanism in P. monodon, we have investigated the both the transcriptomes and regulatory mechanisms of the eyestalk ablated ovary developmental stages with RNA-seq and ATACseq. For RNA-seq data, a total of 182.29 G of clean data was obtained, with the effective data distribution for each sample ranging from 6.42 to 7.04 G. Meanwhile, the differentially expressed genes (DEGs) between ovarian development stages were identified. We examined, through DEG enrichment analysis, eyestalk gene expression patterns for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. We also identified a variety of accessible regions that appear to be differentially expressed throughout ovarian maturation. These include Motifs that encode bZIP and C2H2 transciption factor families. Furthermore, by integrating DEGs and differential accessible regions, we found HIF-signaling pathway, AGE-RAGE signaling pathway, ABC transporters pathways, were involved during ovarian maturation. Our study offers new insights into the role of the eyestalk in the regulation of reproduction, providing a basis for selective breeding and exploring alternative methods to induce ovary maturation.
{"title":"Integrated landscape of accessible regions and transcriptomic profiles in Penaeus monodon during ovarian maturation","authors":"Yundong Li , Jing An , Shigui Jiang , Song Jiang , JianHua Huang , LiShi Yang , Qibin Yang , Jianzhi Shi , Zhenhua Ma , Falin Zhou","doi":"10.1016/j.aquaculture.2025.742513","DOIUrl":"10.1016/j.aquaculture.2025.742513","url":null,"abstract":"<div><div>The ovary maturation process poses a significant constraint on the commercial production and breeding of <em>Penaeus monodon</em>, one of the most extensively cultivated crustacean species. The reproductive stage is controlled by neuropeptide hormones and various proteins released from secretory sites (X-organ/sinus gland, XO/SG) within the eyestalk. Unilateral eyestalk ablation has been a widely employed method to artificially induce ovarian maturation in farmed <em>P. monodon</em>. To better understand the reproductive regulation mechanism in <em>P. monodon</em>, we have investigated the both the transcriptomes and regulatory mechanisms of the eyestalk ablated ovary developmental stages with RNA-seq and ATACseq. For RNA-seq data, a total of 182.29 G of clean data was obtained, with the effective data distribution for each sample ranging from 6.42 to 7.04 G. Meanwhile, the differentially expressed genes (DEGs) between ovarian development stages were identified. We examined, through DEG enrichment analysis, eyestalk gene expression patterns for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. We also identified a variety of accessible regions that appear to be differentially expressed throughout ovarian maturation. These include Motifs that encode bZIP and C2H2 transciption factor families. Furthermore, by integrating DEGs and differential accessible regions, we found HIF-signaling pathway, AGE-RAGE signaling pathway, ABC transporters pathways, were involved during ovarian maturation. Our study offers new insights into the role of the eyestalk in the regulation of reproduction, providing a basis for selective breeding and exploring alternative methods to induce ovary maturation.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742513"},"PeriodicalIF":3.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-02DOI: 10.1016/j.aquaculture.2025.742520
Xinnan Zhou , Yunshan Qiu , Chen Li , Ya Huang , Ping Ouyang , Defang Chen , Yi Geng , Xiaoli Huang
Nocardia seriolae (N. seriolae) is the primary pathogen identified in Micropterus salmoides, causing nocardiosis, which results in substantial mortality. Although numerous studies have been carried out, there is currently no effective method for the early diagnosis of it to facilitate timely treatment. In this study, we used histological methods to comprehensively analyze the histopathological alterations in M. salmoides infected with N. seriolae at different infection stages to observe the dynamic pathological changes and explore the earliest affected organ. Additionally, we investigated the expression of genes related to inflammation and macrophage immunity in the infected tissues using qRT-PCR. Meanwhile, a duplex PCR method was developed. The results found that the histopathological process could be categorized into three stages: early (latent) stage, middle (reaction) stage, and late (terminal) stage depending on a histopathological scoring system. The genes related to inflammation and macrophage immunity expressed earlier before histopathology, which are precursor factors for granuloma formation. Among all tissues, the head kidney was one of the earliest tissue which was found white nodules with N. seriolae invading. Therefore, the head kidney tissue was selected as the mainly early diagnosis sample for duplex PCR test. Additionally, the sensitivity of the duplex PCR could reach 1.8764 × 10−1 ng/μL. These findings provide a reference for the dynamic process of its pathogenesis, the mechanism of granuloma formation, and established a sensitive duplex PCR method by using the most earliest affected tissue as early diagnosis sample, offering new ideas for the control of N. seriolae.
{"title":"The pathological characteristics at different stages and early diagnosis by duplex PCR of Nocardia seriolae disease in largemouth bass (Micropterus salmoides)","authors":"Xinnan Zhou , Yunshan Qiu , Chen Li , Ya Huang , Ping Ouyang , Defang Chen , Yi Geng , Xiaoli Huang","doi":"10.1016/j.aquaculture.2025.742520","DOIUrl":"10.1016/j.aquaculture.2025.742520","url":null,"abstract":"<div><div><em>Nocardia seriolae</em> (<em>N. seriolae</em>) is the primary pathogen identified in <em>Micropterus salmoides</em>, causing nocardiosis, which results in substantial mortality. Although numerous studies have been carried out, there is currently no effective method for the early diagnosis of it to facilitate timely treatment. In this study, we used histological methods to comprehensively analyze the histopathological alterations in <em>M. salmoides</em> infected with <em>N. seriolae</em> at different infection stages to observe the dynamic pathological changes and explore the earliest affected organ. Additionally, we investigated the expression of genes related to inflammation and macrophage immunity in the infected tissues using qRT-PCR. Meanwhile, a duplex PCR method was developed. The results found that the histopathological process could be categorized into three stages: early (latent) stage, middle (reaction) stage, and late (terminal) stage depending on a histopathological scoring system. The genes related to inflammation and macrophage immunity expressed earlier before histopathology, which are precursor factors for granuloma formation. Among all tissues, the head kidney was one of the earliest tissue which was found white nodules with <em>N. seriolae</em> invading. Therefore, the head kidney tissue was selected as the mainly early diagnosis sample for duplex PCR test. Additionally, the sensitivity of the duplex PCR could reach 1.8764 × 10<sup>−1</sup> ng/μL. These findings provide a reference for the dynamic process of its pathogenesis, the mechanism of granuloma formation, and established a sensitive duplex PCR method by using the most earliest affected tissue as early diagnosis sample, offering new ideas for the control of <em>N. seriolae</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742520"},"PeriodicalIF":3.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-02DOI: 10.1016/j.aquaculture.2025.742521
Hong Guo , Tao Liu , Hongtao Nie
High temperatures are considered one of the major environmental factors contributing to the mass death of clams in summer, and prolonged high temperatures may increase their susceptibility to bacteria. We used the Illumina HiSeq platform to perform transcriptome sequencing on clams treated with heat stress (H), Vibrio anguillarum infection (NV), and a combination of heat stress and V. anguillarum (HV). Twelve differentially expressed genes (DEGs) related to anti-oxidation (GPx-2, GST-C, HSP70B2, HSP90), immune (Big defensin, TBK1, TRIM56, TRAF3) and apoptosis (EGR-1, BCL-3, BIRC7-a, CASP-3) were verified by real-time quantitative PCR (RT-qPCR). In addition, KEGG pathway enrichment analysis showed that, compared with the control group, the V. anguillarum infection group under high-temperature conditions exhibited significant enrichment in lysosome, autophagy, and phagosome pathway genes. The expression levels of lysosome and phagosome-related genes decreased significantly in R. philippinarum treated with high temperatures and V. anguillarum, while autophagy-related genes increased significantly. Therefore, our results suggest that long-term high temperatures inhibit the immune homeostasis regulation mechanisms mediated by the lysosomal and phagosomal pathways, while enhancing autophagy function, which may be related to the large-scale death of R. philippinarum in summer. The results of this study provide new insights into the immune response of the Manila clam under high temperature conditions when infected with V. anguillarum, offering a reference for further research into the molecular mechanisms of immune resistance in R. philippinarum.
{"title":"Molecular insights into the immune response of Ruditapes philippinarum under Vibrio anguillarum infection at high temperature","authors":"Hong Guo , Tao Liu , Hongtao Nie","doi":"10.1016/j.aquaculture.2025.742521","DOIUrl":"10.1016/j.aquaculture.2025.742521","url":null,"abstract":"<div><div>High temperatures are considered one of the major environmental factors contributing to the mass death of clams in summer, and prolonged high temperatures may increase their susceptibility to bacteria. We used the Illumina HiSeq platform to perform transcriptome sequencing on clams treated with heat stress (H), <em>Vibrio anguillarum</em> infection (NV), and a combination of heat stress and <em>V. anguillarum</em> (HV). Twelve differentially expressed genes (DEGs) related to anti-oxidation (<em>GPx-2, GST-C, HSP70B2, HSP90</em>), immune (<em>Big defensin, TBK1, TRIM56, TRAF3</em>) and apoptosis (<em>EGR-1, BCL-3, BIRC7-a, CASP-3</em>) were verified by real-time quantitative PCR (RT-qPCR). In addition, KEGG pathway enrichment analysis showed that, compared with the control group, the <em>V. anguillarum</em> infection group under high-temperature conditions exhibited significant enrichment in lysosome, autophagy, and phagosome pathway genes. The expression levels of lysosome and phagosome-related genes decreased significantly in <em>R. philippinarum</em> treated with high temperatures and <em>V. anguillarum</em>, while autophagy-related genes increased significantly. Therefore, our results suggest that long-term high temperatures inhibit the immune homeostasis regulation mechanisms mediated by the lysosomal and phagosomal pathways, while enhancing autophagy function, which may be related to the large-scale death of <em>R. philippinarum</em> in summer. The results of this study provide new insights into the immune response of the Manila clam under high temperature conditions when infected with <em>V. anguillarum</em>, offering a reference for further research into the molecular mechanisms of immune resistance in <em>R. philippinarum</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742521"},"PeriodicalIF":3.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.aquaculture.2025.742514
Hongmei Yuan , Junxian Li , Haihang Wang , Regan Nicholaus , Muhammad Naeem Ramzan , Wen Yang , Zhongming Zheng , Yangcai Wang
As the aquaculture industry continues to grow rapidly, the environmental consequences of wastewater discharge from aquaculture have become a major concern. The biological treatment method has proven to be an effective and beneficial approach for wastewater treatment. In this experiment, aquaculture wastewater was treated using two different ratios of Bacillus subtilis and Bacillus licheniformis composites in combination with sea purslane (Sesuvium portulacastrum) to investigate the wastewater treatment efficiency and microbial community. Sea purslane effectively removed nutrients from aquaculture wastewater. The addition of compound Bacillus significantly increased the removal rates of CODMn, TP, and PO43−-P, which were 54.9–56.4 %, 78.2–79.9 %, and 85.0–89.6 %, respectively. Moreover, the compound Bacillus notably influenced the microbial community composition in the water and root systems, reducing species abundance while increasing community diversity, with Treatment 2 exhibiting the highest community stability. The addition of the compound Bacillus significantly increased the relative abundance of Alphaproteobacteria. Most of the important operational taxonomic units (OTUs) that significantly impacted the groups belonged to Alphaproteobacteria. Different biomarkers in the water and root system were primarily from Alphaproteobacteria, Gammaproteobacteria, and Bacteroidia, including Idiomarina and Alteromonas, Ruegeria, Vibrio, Algoriphagus, and Maribacter, among others. These microorganisms play a significant role in promoting the removal rate of nitrogen and phosphorus. Furthermore, the compound Bacillus notably affected the abundance of nitrogen cycle-related sequences and increased the relative abundance of microorganisms associated with the nitrogen cycle. These findings provide valuable insights for optimizing aquaculture wastewater treatment processes.
{"title":"The compound Bacillus and sea purslane (Sesuvium portulacastrum) enhanced aquaculture wastewater treatment efficiency: Insights from a study on microbial community distributions","authors":"Hongmei Yuan , Junxian Li , Haihang Wang , Regan Nicholaus , Muhammad Naeem Ramzan , Wen Yang , Zhongming Zheng , Yangcai Wang","doi":"10.1016/j.aquaculture.2025.742514","DOIUrl":"10.1016/j.aquaculture.2025.742514","url":null,"abstract":"<div><div>As the aquaculture industry continues to grow rapidly, the environmental consequences of wastewater discharge from aquaculture have become a major concern. The biological treatment method has proven to be an effective and beneficial approach for wastewater treatment. In this experiment, aquaculture wastewater was treated using two different ratios of <em>Bacillus subtilis</em> and <em>Bacillus licheniformis</em> composites in combination with sea purslane (<em>Sesuvium portulacastrum</em>) to investigate the wastewater treatment efficiency and microbial community. Sea purslane effectively removed nutrients from aquaculture wastewater. The addition of compound <em>Bacillus</em> significantly increased the removal rates of COD<sub>Mn</sub>, TP, and PO<sub>4</sub><sup>3−</sup>-P, which were 54.9–56.4 %, 78.2–79.9 %, and 85.0–89.6 %, respectively. Moreover, the compound <em>Bacillus</em> notably influenced the microbial community composition in the water and root systems, reducing species abundance while increasing community diversity, with Treatment 2 exhibiting the highest community stability. The addition of the compound <em>Bacillus</em> significantly increased the relative abundance of Alphaproteobacteria. Most of the important operational taxonomic units (OTUs) that significantly impacted the groups belonged to Alphaproteobacteria. Different biomarkers in the water and root system were primarily from Alphaproteobacteria, Gammaproteobacteria, and Bacteroidia, including <em>Idiomarina</em> and <em>Alteromonas</em>, <em>Ruegeria</em>, <em>Vibrio</em>, <em>Algoriphagus</em>, and <em>Maribacter</em>, among others. These microorganisms play a significant role in promoting the removal rate of nitrogen and phosphorus. Furthermore, the compound <em>Bacillus</em> notably affected the abundance of nitrogen cycle-related sequences and increased the relative abundance of microorganisms associated with the nitrogen cycle. These findings provide valuable insights for optimizing aquaculture wastewater treatment processes.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742514"},"PeriodicalIF":3.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.aquaculture.2025.742509
Yuqing Zhang , Wenying Zhang , Ao Li , Yanmin Jian , Shujing Zhang , Dongwu Liu , Xungang Tan , Feng You , Qiuxiang Pang , Shuang Jiao
Olive flounder (Paralichthys olivaceus), a valuable mariculture species, exhibits a significant female-biased sexual size dimorphism (SSD). Despite its economic and biological importance, the molecular mechanisms underlying this phenomenon, in particular the contribution of non-coding RNAs (ncRNAs) and their associated competitive endogenous RNA (ceRNA) regulatory mechanisms, remain unclear. In this study, we conducted whole transcriptome sequencing of the brains/pituitaries and muscles of olive flounder during the critical period for SSD establishment, identifying 23/282/85 and 25/193/82 differentially expressed (DE) miRNAs/circRNAs/lncRNAs, respectively. Based on the regulatory interactions among these DE RNAs, we constructed the lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA ceRNA networks. Our analyses revealed crucial SSD-related pathways regulated by these ncRNAs/ceRNA networks, including “ovarian steroidogenesis”, “prolactin signaling pathway”, “cell adhesion molecules”, “GABAergic synapse”, and “dopaminergic synapse” pathways in brains/pituitaries, “ECM-receptor interaction”, “PI3K-Akt signaling pathway”, “focal adhesion”, “regulation of actin cytoskeleton”, and several metabolism-related pathways in muscles. Moreover, numerous genes regulated by these ncRNAs/ceRNA networks emerged as potential candidates for SSD establishment, such as frizzled3 (fzd3), cholecystokinin A receptor (cckar), carbonic anhydrase 4 (ca4), and calcium voltage-gated channel subunit alpha1 B (cacna1b) in brains/pituitaries, and DNA (cytosine-5) methyltransferase 3A (dnmt3a), Krüppel-like factor 11 (klf11), myocyte enhancer factor 2c (mef2c), insulin receptor (insr), serum- and glucocorticoid-inducible kinase 1 (sgk1), nuclear receptor 4A1 (nr4a1), and apelin in muscles. This study provides the first preliminary insights into the ncRNAs and their ceRNA networks implicated in SSD-related growth traits in flounder, providing potential targets for selective breeding programs aimed at enhancing growth in aquaculture.
{"title":"Identification of miRNAs, lncRNAs, circRNAs, and their ceRNA networks in the establishment of sexual size dimorphism in the olive flounder (Paralichthys olivaceus)","authors":"Yuqing Zhang , Wenying Zhang , Ao Li , Yanmin Jian , Shujing Zhang , Dongwu Liu , Xungang Tan , Feng You , Qiuxiang Pang , Shuang Jiao","doi":"10.1016/j.aquaculture.2025.742509","DOIUrl":"10.1016/j.aquaculture.2025.742509","url":null,"abstract":"<div><div>Olive flounder (<em>Paralichthys olivaceus</em>), a valuable mariculture species, exhibits a significant female-biased sexual size dimorphism (SSD). Despite its economic and biological importance, the molecular mechanisms underlying this phenomenon, in particular the contribution of non-coding RNAs (ncRNAs) and their associated competitive endogenous RNA (ceRNA) regulatory mechanisms, remain unclear. In this study, we conducted whole transcriptome sequencing of the brains/pituitaries and muscles of olive flounder during the critical period for SSD establishment, identifying 23/282/85 and 25/193/82 differentially expressed (DE) miRNAs/circRNAs/lncRNAs, respectively. Based on the regulatory interactions among these DE RNAs, we constructed the lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA ceRNA networks. Our analyses revealed crucial SSD-related pathways regulated by these ncRNAs/ceRNA networks, including “ovarian steroidogenesis”, “prolactin signaling pathway”, “cell adhesion molecules”, “GABAergic synapse”, and “dopaminergic synapse” pathways in brains/pituitaries, “ECM-receptor interaction”, “PI3K-Akt signaling pathway”, “focal adhesion”, “regulation of actin cytoskeleton”, and several metabolism-related pathways in muscles. Moreover, numerous genes regulated by these ncRNAs/ceRNA networks emerged as potential candidates for SSD establishment, such as <em>frizzled3</em> (<em>fzd3</em>), <em>cholecystokinin A receptor</em> (<em>cckar</em>), <em>carbonic anhydrase 4</em> (<em>ca4</em>), and <em>calcium voltage-gated channel subunit alpha1 B</em> (<em>cacna1b</em>) in brains/pituitaries, and <em>DNA (cytosine-5) methyltransferase 3A</em> (<em>dnmt3a</em>), <em>Krüppel-like factor 11</em> (<em>klf11</em>), <em>myocyte enhancer factor 2c</em> (<em>mef2c</em>), <em>insulin receptor</em> (<em>insr</em>), <em>serum- and glucocorticoid-inducible kinase 1</em> (<em>sgk1</em>), <em>nuclear receptor 4A1</em> (<em>nr4a1</em>), and <em>apelin</em> in muscles. This study provides the first preliminary insights into the ncRNAs and their ceRNA networks implicated in SSD-related growth traits in flounder, providing potential targets for selective breeding programs aimed at enhancing growth in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742509"},"PeriodicalIF":3.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.aquaculture.2025.742506
Zexun Zhou , Ye Yuan , YunYun Liu , Shandong Chen , Yongchun Li , Yan Miao , Shi Wang , Zhongyuan Shen , Lei Zeng , Li Ren , Chang Wu , Qizhi Liu , Qinbo Qin , Wuhui Li , Shaojun Liu
The aim of this study was to explore the effects of dietary bile acids (BAs) supplementation on herbivorous diet adaptation in allodiploid hybrid fish derived from blunt snout bream (♀) × topmouth culter (♂). Three experimental diets were formulated: a commercial basal diet (CG group), a basal diet supplement with duckweed (H group), and a basal diet supplemented with duckweed and 600 mg kg−1 BA (T group). After 56 days of feeding, the growth parameters increased, and greater intestinal cellulase activity was detected in the T group. Moreover, the T group fish presented a longer and denser small intestinal villi. In general, the characteristics of the H group were intermediate between those of the other two groups (T > H > CG). Compared with CG group fish, 711 specific differentially expressed genes (DEGs) were identified in the intestine of T group fish. The upregulated DEGs were primarily enriched in MAPK signaling pathway, protein digestion and absorption, and mTOR signaling pathway; the downregulated DEGs were predominantly enriched in steroid hormone biosynthesis, fatty acid metabolism and primary bile acid biosynthesis. Besides, several upregulated genes, such as jak, irs, egfr, prss, and cpa which related to cell proliferation and differentiation, digestion, and metabolism, are associated with the changes in intestinal histomorphology. These changes could promote the intestinal adaptation of an herbivorous diet. The metagenome sequencing results revealed that exogenous BAs significantly changed the structure of the gut microbiota; decreased the abundance of potentially pathogenic bacteria or fungi such as Proteobacteria, Mucoromycota, and Pseudomonas; and increased the abundance of probiotics such as the cellulose-producing bacteria Micromonospora. In addition, functional analysis revealed that the abundance of some enzyme families related to cellulose degradation was significantly greater in the T group. These findings suggested that exogenous BAs influences host intestinal gene expression and the structure of the gut microbiota via the gut microbiota-bile acid pathway, which in turn contributes to herbivorous diet adaptation in juvenile allodiploid hybrid fish.
{"title":"Dietary bile acid supplementation alters the gut transcriptome and metagenome and contributes to herbivorous diet adaptation in juvenile allodiploid hybrid fish","authors":"Zexun Zhou , Ye Yuan , YunYun Liu , Shandong Chen , Yongchun Li , Yan Miao , Shi Wang , Zhongyuan Shen , Lei Zeng , Li Ren , Chang Wu , Qizhi Liu , Qinbo Qin , Wuhui Li , Shaojun Liu","doi":"10.1016/j.aquaculture.2025.742506","DOIUrl":"10.1016/j.aquaculture.2025.742506","url":null,"abstract":"<div><div>The aim of this study was to explore the effects of dietary bile acids (BAs) supplementation on herbivorous diet adaptation in allodiploid hybrid fish derived from blunt snout bream (♀) × topmouth culter (♂). Three experimental diets were formulated: a commercial basal diet (CG group), a basal diet supplement with duckweed (H group), and a basal diet supplemented with duckweed and 600 mg kg<sup>−1</sup> BA (T group). After 56 days of feeding, the growth parameters increased, and greater intestinal cellulase activity was detected in the T group. Moreover, the T group fish presented a longer and denser small intestinal villi. In general, the characteristics of the H group were intermediate between those of the other two groups (T > H > CG). Compared with CG group fish, 711 specific differentially expressed genes (DEGs) were identified in the intestine of T group fish. The upregulated DEGs were primarily enriched in MAPK signaling pathway, protein digestion and absorption, and mTOR signaling pathway; the downregulated DEGs were predominantly enriched in steroid hormone biosynthesis, fatty acid metabolism and primary bile acid biosynthesis. Besides, several upregulated genes, such as <em>jak</em>, <em>irs</em>, <em>egfr</em>, <em>prss</em>, and <em>cpa</em> which related to cell proliferation and differentiation, digestion, and metabolism, are associated with the changes in intestinal histomorphology. These changes could promote the intestinal adaptation of an herbivorous diet. The metagenome sequencing results revealed that exogenous BAs significantly changed the structure of the gut microbiota; decreased the abundance of potentially pathogenic bacteria or fungi such as <em>Proteobacteria</em>, <em>Mucoromycota,</em> and <em>Pseudomonas</em>; and increased the abundance of probiotics such as the cellulose-producing bacteria <em>Micromonospora</em>. In addition, functional analysis revealed that the abundance of some enzyme families related to cellulose degradation was significantly greater in the T group. These findings suggested that exogenous BAs influences host intestinal gene expression and the structure of the gut microbiota via the gut microbiota-bile acid pathway, which in turn contributes to herbivorous diet adaptation in juvenile allodiploid hybrid fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742506"},"PeriodicalIF":3.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-31DOI: 10.1016/j.aquaculture.2025.742496
M. Junaid Sidiq , Alexander Ciji , Ananya Khatei , C. Siva , Narinder Kumar Chadha , Paramita Banerjee Sawant , Pramod Kumar Pandey , M.S. Akhtar
The present study used a mid-to-high altitude dwelling migratory fish, Tor putitora, as a model for studying the impact of ambient UVB on flourishing fish culture in the high-altitudinal areas of the Indian Himalayas. For this, two sets of the juveniles of T. putitora (22 ± 6.3 g) were exposed for 60 days to two ambient UVB levels- 1.0 and 1.5 W/m2 (T1 and T2). The two levels corresponded to about 24.0 % and 36.0 % of the daily average UVB incidence of the peak noon hours (1100 to 1300) of the summer. A third, unexposed set served as control (CT). After the experimental trial, the fish were evaluated for fitness indices, including thermal tolerance and respiratory metabolic rates. Important physiological, genotoxic, molecular and histological changes were also evaluated in the blood plasma and body tissues. Among the welfare indices, routine and maximum metabolic rates and lower critical temperature increased, whereas thermal scope decreased in the UVB-exposed fish. The exposure caused a significant increase in the total antioxidative capacity (TAC), nitric oxide (NO), cholesterol, cortisol, and aminotransferases in the plasma. However, the antioxidative enzyme levels significantly decreased, whereas malonaldehyde levels increased in the kidney and liver of the UVB-exposed fish, dose-dependently. In addition, the dehydrogenase enzyme (LDH and MDH) activities significantly increased in the liver, kidney, and muscle, dose-dependently. Among the non-specific immune parameters, plasma lysozyme, neutrophil respiratory burst activity, and total blood leucocyte count also increase dose-dependently. Molecular analysis revealed that the antioxidative pathway genes (gst, cat1, sod1, and cyp1a1) and immune gene (nf-κb2) were differentially expressed in the liver, kidney, and muscle tissues of the UVB-exposed fish, showing an overall downregulatory trend. The cortisol-related gene, hsd11b1a, was upregulated in the kidney and downregulated in the liver of the UVB-exposed fish. Moreover, UVB exposure resulted in structural, non-pathogenic changes in the gill, liver, and kidney. Our study showed that although prolonged exposure to ambient UVB radiations did not affect the physiology of the fish beyond their threshold, it did increase the energetic costs and affected the antioxidative mechanisms. The results indicate that any further increase in the environmental UVB can harm the welfare of the fish, both in the wild and captivity, in high-altitude regions.
{"title":"Exposure to ambient UVB light influences the welfare, energy requirements, metabolism, and relevant molecular biology of the Himalayan fish Tor putitora: Investigating the hidden costs of sunlight in upland aquaculture","authors":"M. Junaid Sidiq , Alexander Ciji , Ananya Khatei , C. Siva , Narinder Kumar Chadha , Paramita Banerjee Sawant , Pramod Kumar Pandey , M.S. Akhtar","doi":"10.1016/j.aquaculture.2025.742496","DOIUrl":"10.1016/j.aquaculture.2025.742496","url":null,"abstract":"<div><div>The present study used a mid-to-high altitude dwelling migratory fish, <em>Tor putitora</em>, as a model for studying the impact of ambient UVB on flourishing fish culture in the high-altitudinal areas of the Indian Himalayas. For this, two sets of the juveniles of <em>T. putitora</em> (22 ± 6.3 g) were exposed for 60 days to two ambient UVB levels- 1.0 and 1.5 W/m<sup>2</sup> (T1 and T2). The two levels corresponded to about 24.0 % and 36.0 % of the daily average UVB incidence of the peak noon hours (1100 to 1300) of the summer. A third, unexposed set served as control (CT). After the experimental trial, the fish were evaluated for fitness indices, including thermal tolerance and respiratory metabolic rates. Important physiological, genotoxic, molecular and histological changes were also evaluated in the blood plasma and body tissues. Among the welfare indices, routine and maximum metabolic rates and lower critical temperature increased, whereas thermal scope decreased in the UVB-exposed fish. The exposure caused a significant increase in the total antioxidative capacity (TAC), nitric oxide (NO), cholesterol, cortisol, and aminotransferases in the plasma. However, the antioxidative enzyme levels significantly decreased, whereas malonaldehyde levels increased in the kidney and liver of the UVB-exposed fish, dose-dependently. In addition, the dehydrogenase enzyme (LDH and MDH) activities significantly increased in the liver, kidney, and muscle, dose-dependently. Among the non-specific immune parameters, plasma lysozyme, neutrophil respiratory burst activity, and total blood leucocyte count also increase dose-dependently. Molecular analysis revealed that the antioxidative pathway genes (<em>gst, cat1, sod1,</em> and <em>cyp1a1</em>) and immune gene (<em>nf-κb2</em>) were differentially expressed in the liver, kidney, and muscle tissues of the UVB-exposed fish, showing an overall downregulatory trend. The cortisol-related gene, <em>hsd11b1a</em>, was upregulated in the kidney and downregulated in the liver of the UVB-exposed fish. Moreover, UVB exposure resulted in structural, non-pathogenic changes in the gill, liver, and kidney. Our study showed that although prolonged exposure to ambient UVB radiations did not affect the physiology of the fish beyond their threshold, it did increase the energetic costs and affected the antioxidative mechanisms. The results indicate that any further increase in the environmental UVB can harm the welfare of the fish, both in the wild and captivity, in high-altitude regions.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742496"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-31DOI: 10.1016/j.aquaculture.2025.742500
Raquel Quirós-Pozo , Christian Monzón , Sarah Montesdeoca-Esponda , María Esther Torres-Padrón , Javier Roo
Infestations by Neobenedenia girellae constitute significant challenges to the culture of greater amberjack (Seriola dumerili), a promising species for aquaculture diversification. The impact of these infestations is further amplified by global ocean warming, underscoring the urgent need for effective prevention and control measures. This study assessed the efficacy of different praziquantel (PZQ) treatments on growth performance, muscle kinetic profiles (Experiment 1), and their ability to control N. girellae infestations in S. dumerili juveniles reared in an open seawater system (Experiment 2). In Experiment 1, six treatments were tested: T1 (Control), T2-PZQ 2.25 %-1 (450 mg PZQ/kg live weight, single dose), T3-PZQ 1.125 %-2 (225 mg PZQ/kg live weight, 2 days), T4-PZQ 0.75 %-3 (150 mg PZQ/kg live weight, 3 days), T5-PZQ 0.75 %-2 (150 mg PZQ/kg live weight, 2 days), and T6-PZQ 0.75 %-1 (150 mg PZQ/kg live weight, single dose). In Experiment 2, protocols T1–T4 were evaluated. Results indicated that growth performance was unaffected by PZQ treatments, and muscle PZQ concentrations returned to baseline within 48 h post-administration. Protocols T3–1.125 %-2 and T4–0.75 %-3 exhibited the highest efficacy against N. girellae adults, achieving 91 % and 99.8 % parasite reduction, respectively. The protocol involving 150 mg PZQ/kg administered over three consecutive days proved to be the most effective for managing N. girellae infestations, without compromising fish growth and with minimal muscle residue persistence.
{"title":"Effective control of Neobenedenia girellae infestation by an optimized oral protocol in greater amberjack juveniles (Seriola dumerili)","authors":"Raquel Quirós-Pozo , Christian Monzón , Sarah Montesdeoca-Esponda , María Esther Torres-Padrón , Javier Roo","doi":"10.1016/j.aquaculture.2025.742500","DOIUrl":"10.1016/j.aquaculture.2025.742500","url":null,"abstract":"<div><div>Infestations by <em>Neobenedenia girellae</em> constitute significant challenges to the culture of greater amberjack (<em>Seriola dumerili</em>), a promising species for aquaculture diversification. The impact of these infestations is further amplified by global ocean warming, underscoring the urgent need for effective prevention and control measures. This study assessed the efficacy of different praziquantel (PZQ) treatments on growth performance, muscle kinetic profiles (Experiment 1), and their ability to control <em>N. girellae</em> infestations in <em>S. dumerili</em> juveniles reared in an open seawater system (Experiment 2). In Experiment 1, six treatments were tested: T1 (Control), T2-PZQ 2.25 %-1 (450 mg PZQ/kg live weight, single dose), T3-PZQ 1.125 %-2 (225 mg PZQ/kg live weight, 2 days), T4-PZQ 0.75 %-3 (150 mg PZQ/kg live weight, 3 days), T5-PZQ 0.75 %-2 (150 mg PZQ/kg live weight, 2 days), and T6-PZQ 0.75 %-1 (150 mg PZQ/kg live weight, single dose). In Experiment 2, protocols T1–T4 were evaluated. Results indicated that growth performance was unaffected by PZQ treatments, and muscle PZQ concentrations returned to baseline within 48 h post-administration. Protocols T3–1.125 %-2 and T4–0.75 %-3 exhibited the highest efficacy against <em>N. girellae</em> adults, achieving 91 % and 99.8 % parasite reduction, respectively. The protocol involving 150 mg PZQ/kg administered over three consecutive days proved to be the most effective for managing <em>N. girellae</em> infestations, without compromising fish growth and with minimal muscle residue persistence.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742500"},"PeriodicalIF":3.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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