Aquaculture最新文献

{"title":"Comparative transcriptome analysis reveals distinct immune response in different ploidy cyprinid caudal fin cells following SVCV infection","authors":"Dan Zeng ,&nbsp;Kai Yao ,&nbsp;Yixin Chen ,&nbsp;Yingbing Wei ,&nbsp;Hui Wu ,&nbsp;Jun Xiao ,&nbsp;Hao Feng","doi":"10.1016/j.aquaculture.2025.742365","DOIUrl":"10.1016/j.aquaculture.2025.742365","url":null,"abstract":"<div><div>Triploid fish (3n = 150) exhibit enhanced disease resistance compared to their parents—red crucian carp (♀, 2n = 100) and allotetraploid (♂, 4n = 200). To elucidate the innate immunity of triploid hybrid, a comparative transcriptomic analysis was carried out to elucidate the molecular events during spring viraemia of carp virus (SVCV) infection in caudal fin cell from the red crucian carp, triploid hybrid and allotetraploid, denoted as G2n, G3n, and G4n, respectively. KEGG enrichment analysis indicated that SVCV may alter the host cell's metabolic profiles post-infection, and the ECM-mediated adhesion plaque signaling pathway may facilitate the virus's recognition and infection processes. Distinct signaling regulation mechanisms in response to SVCV infection were observed across different ploidy levels. In G2n, immune-related pathways such as the TNF signaling pathway and Toll-like receptor signaling pathway were activated. The downregulation of immune related gene, such as <em>MHC I</em>, may indicate viral strategies to evade detection by the immune system through inhibited antigen presentation in diploid fish. Apoptosis-related processes including the Apoptosis, Phagosome, Rap1 signaling pathway, and Hippo signaling pathway were significantly enriched in G4n, indicating SVCV hijack the host autophagy and apoptosis to improve their replication and spreading. The outcomes of the comparative analysis demonstrate that, in G3n, robust protective barriers against SVCV invasion were established through the downregulation of <em>MMP9</em>, <em>mTOR</em>, <em>p53</em>, and <em>NCAM</em>, alongside the upregulation of <em>MHC I</em>, <em>CLDN</em>, and <em>CAV 1</em>-mediated interactions. This coordinated multi-gene response might be a key factor contributing to the enhanced resistance of triploid fish. The findings underscore the distinct signal regulation of different ploidy cyprinid fish in response to SVCV infection, providing valuable reference data for the prevention and treatment of SVCV.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742365"},"PeriodicalIF":3.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics analysis reveals the regulatory effect of exosomes on innate immunity in Chlamys farreri","authors":"Shengwen Li ,&nbsp;Lihan Wang ,&nbsp;Yansong Hou ,&nbsp;Ying Guo ,&nbsp;Mengqiang Wang","doi":"10.1016/j.aquaculture.2025.742374","DOIUrl":"10.1016/j.aquaculture.2025.742374","url":null,"abstract":"<div><div>Exosomes serve as an additional medium for inter- and intracellular communication, facilitating a range of biological processes in organisms through the transfer of bioactive substances. When shellfish responds to external stimuli, exosomes may regulate important immune responses by interacting with immune regulatory elements. However, the potential molecular basis between exosomes and innate immune in mollusks regulation remains to be explored. In this study, we used tandem mass tag (TMT) labeling technology to analyze the protein expression profiles of hemocytes stimulated by different sources of exosomes in <em>Chlamys farreri</em>, including LPS-derived exosomes stimulation group, PBS stimulation group, and untreated exosomes stimulation group. The results showed that the differentially expressed proteins (DEPs) screened in the LPS-stimulated group contained a variety of common immune-associated proteins, such as mono-ADP-ribosyltransferase PARP14 (PARP14), Dynein light chain cytoplasmic (Dynll2), Baculoviral IAP repeat-containing 1 (NAIP), MORC family CW-type zinc finger 3 (MORC3), ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase (CD38), and Calmodulin (CALM), compared to the other groups. The GO and KEGG functional annotation analysis showed that DEPs were involved in key biological functions and pathways, including cellular processes, response to stimulus, immune system process and autophagy functions, as well as signaling pathways such as the IL-17 Signaling Pathway, Phagosome, and Apoptosis. PPI network analysis revealed that TUBB4B and the down-regulated protein ACTB may act as important central regulatory proteins involved in the regulation of innate immune function. The results further indicated that exosomes were involved in the innate immune regulation by mediating the interaction between regulatory elements during cell communication. All these results demonstrate that exosomes play a core regulatory role in the innate immune defense mechanism of marine invertebrates, and also provide fundamental data for disease prevention and control and the breeding of disease-resistant cultured shellfish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742374"},"PeriodicalIF":3.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long term effects of smolt and post-smolt production strategy on mortality, growth, sexual maturation and melanized focal changes in farmed Atlantic salmon (Salmo salar L.)","authors":"Lars Eirik Myklatun ,&nbsp;Angelico Madaro ,&nbsp;Antony Jesu Prabhu Philip ,&nbsp;Audun Ø. Pedersen ,&nbsp;Sofie Remø ,&nbsp;Tom Johnny Hansen ,&nbsp;Thomas W.K. Fraser ,&nbsp;Trygve Sigholt ,&nbsp;Sigurd Stefansson ,&nbsp;Per Gunnar Fjelldal","doi":"10.1016/j.aquaculture.2025.742371","DOIUrl":"10.1016/j.aquaculture.2025.742371","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Diet-based smolt production and land-based post-smolt rearing are emerging strategies in Atlantic salmon aquaculture. However, their impact on fish performance and welfare over a full production cycle is poorly understood. To address this, we exposed 0+ Atlantic salmon parr (∼50 g) initially kept on continuous light to two different photoperiods at 12 °C: either a ‘square wave’ regime with 6 weeks of short day (12 h light / 12 h dark) followed by a return to continuous light (hereafter &lt;em&gt;LD-LL&lt;/em&gt;), or continuous light throughout (hereafter &lt;em&gt;LL&lt;/em&gt;). When LD-LL returned to continuous light, half of the LD-LL tanks and half of the LL tanks were provided a salt (&lt;em&gt;S&lt;/em&gt;) enriched diet (5.0 % NaCl, 0.5 % CaCl&lt;sub&gt;2&lt;/sub&gt;) in the expectation that it will aid the seawater transition, establishing four different freshwater (FW) regimes: &lt;em&gt;LD-LL&lt;/em&gt;, &lt;em&gt;LL&lt;/em&gt;, &lt;em&gt;LD-LLS&lt;/em&gt; and &lt;em&gt;LLS&lt;/em&gt;. FW rearing ended 4 weeks after initiation of the different dietary regimes (October). Then, fish from each FW regime (∼100–130 g) were equally distributed between three different post-smolt scenarios in a common garden design: In tanks at 12 °C under LL with 15 ppt or 34 ppt, or directly into sea-cages. This created 12 groups (×4 FW regimes and ×3 post-smolt scenarios). Five months later (March), the post-smolts in tanks on 15 and 34 ppt were transferred into sea-cages (at ∼1 kg). The experiment ended 7 months later (September, at about 3.5 kg). Response parameters were weight and length, mortality, and the prevalence of sexual maturation, vertebral deformities, and melanized focal changes (MFC) in the fillet at harvest. FW diet or photoperiod had no effect on mortality, but mortality was higher in those fish that had been moved directly to sea-cages versus those that remained on land for longer. FW diet did not affect growth, but the size at transfer to sea-cage was greater in LL (∼130 g) than LD-LL smolts (∼100 g). Post-smolt rearing on land in 34 ppt stimulated growth to harvest relative to 15 ppt, however, otherwise there was no effect of FW photoperiod or post-smolt scenario on final weights. Maturity levels were higher following the two land-based regimes (male: 53–68 %, female: 6–20 %) compared to those moved directly to sea-cages as smolts (male: 10–25 %, female: 0 %). LD-LL caused a general increase in male maturation compared to LL, while the S diet increased male maturation in fish directly transferred to sea-cages. MFC prevalence was higher in fish reared on land in 34 ppt (∼9.5 %) relative to those reared on 15 ppt (∼3.1 %), and was also higher in immature (∼6.7 %) fish compared to maturing fish (∼3.3 %). All groups had an equal and relatively high level of vertebral deformities (≥1 def. Vertebra ∼44 %, &gt;10 def. Vertebrae ∼23 % and &gt; 20 def. Vertebrae ∼9 %), mostly ‘cross stitch vertebrae’. Harvest yield (total biomass produced excluding mature fish) was higher (∼1.8 fold) in groups transferred directly to sea-cages as ","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742371"},"PeriodicalIF":3.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and evaluation of nanobodies specific for pirA and pirB from Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease","authors":"Shuoru Shao ,&nbsp;Haoran Yang ,&nbsp;Qifan Zeng ,&nbsp;Jingjie Hu ,&nbsp;Mengqiang Wang","doi":"10.1016/j.aquaculture.2025.742357","DOIUrl":"10.1016/j.aquaculture.2025.742357","url":null,"abstract":"<div><div>Acute hepatopancreatic necrosis disease (AHPND) is a bacterial disease of farmed shrimp caused by <em>Vibrio parahaemolyticus</em>. In recent years, AHPND has caused significant damage to the shrimp aquaculture industry, resulting in a notable decline in shrimp production. Given the lack of effective treatment options, it is crucial to develop an early, quick and accurate method of detecting the AHPND pathogen to help prevent disease in shrimp aquaculture. The present study was aimed to generate and evaluate specific nanobodies against the pathogenic factors of <em>V. parahaemolyticus</em> causing AHPND. Anti-pirA and anti-pirB nanobodies were obtained using phage display technology from a natural phage library derived from alpacas, and subsequently produced via the prokaryotic expression system. The specificity and affinity of the nanobodies were validated through western blot and ELISA. The nanobody colloidal gold test strips for the detection of AHPND have been preliminarily developed using colloidal gold immunochromatography technology. The application of colloidal gold test strips enables the identification of AHPND to be reduced to a few minutes, with satisfactory sensitivity and specificity. The detection limits were 125 μg/mL for pirA and 62.5 μg/mL for pirB. These works provided a rapid, inexpensive, and convenient option for the detection of diseases in shrimp aquaculture production.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742357"},"PeriodicalIF":3.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary trace mineral (Cu, Fe, Mn, Zn, and Se) source and levels on production parameters, fillet quality, mineralization, and skin morphology of 1+ and 0+ Atlantic salmon across regions in Norway","authors":"Marialena Kokkali ,&nbsp;Jens-Erik Dessen ,&nbsp;Lene Sveen ,&nbsp;Arnaud Lefrancois ,&nbsp;Gerrit Timmerhaus ,&nbsp;Elin Kvamme ,&nbsp;Antony J. Prabhu Philip ,&nbsp;Katerina Kousoulaki","doi":"10.1016/j.aquaculture.2025.742375","DOIUrl":"10.1016/j.aquaculture.2025.742375","url":null,"abstract":"<div><div>The shift from marine-based to plant-based ingredients in Atlantic salmon feeds presents a complex challenge due to varying nutrient levels and combinations, including mineral levels. Studying the effects of new feeds in large, slaughter-ready fish poses logistical and financial challenges. Unlike most mineral nutrition research studies, typically conducted under controlled conditions, the current study utilizes large-scale research license experiments, providing a vast amount of systematically gathered information. This approach allows for a more conclusive overview of how mineral supplementation affects salmon production in a commercial setup. This study aimed to explore the practical implications of using different dietary levels of essential trace minerals (Cu, Fe, Mn, Zn, and Se) in either organic or inorganic forms in salmon feeds. A total of 12 full-scale feeding trials were conducted across three locations representative of Norwegian salmon production: Northern, Mid, and Western Norway. Each location included four trials with nutritional interventions replicated for 1+ and 0+ smolt. Results indicated that quality parameters, slaughter yield, and welfare indicators were significantly influenced by smolt age, location, and mineral level. Specifically, reduced Fe levels mitigated prooxidative effects and increased the availability of essential trace minerals, while increased Zn supplementation improved skin welfare. The use of organic minerals resulted in reduced gaping, elevated mineral levels in key tissues, and enhanced production performance. This article highlights the complex interplay between dietary mineral supplementation and fish performance, quality, welfare indicators, and tissue mineralization in commercially produced Atlantic salmon. The findings from this study demonstrate the critical role of trace minerals in improving mineral availability and overall fish welfare, underscoring the importance of considering multiple factors in formulating optimal feed compositions for enhancing fish health, performance, and welfare in aquaculture practices. These insights offer significant advancements over previous studies by providing practical solutions to the challenges of modern feed formulations and their impacts on Atlantic salmon production.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742375"},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of starvation-refeeding on antioxidant status, metabolic function, and adaptive response in the muscle of Cyprinus carpio","authors":"Wenrong Feng ,&nbsp;Jianlin Li ,&nbsp;Yuanfeng Xu ,&nbsp;Gang Jiang ,&nbsp;Shengyan Su ,&nbsp;Zhihua Zhang ,&nbsp;Rui Jia ,&nbsp;Yongkai Tang","doi":"10.1016/j.aquaculture.2025.742372","DOIUrl":"10.1016/j.aquaculture.2025.742372","url":null,"abstract":"<div><div>The starvation-refeeding in fish has garnered significant attention, yet the molecular mechanisms underlying the treatment remain incompletely understood. This study aimed to evaluate the alterations in antioxidant status, metabolic function, and adaptive responses in <em>Cyprinus carpio</em> after starvation-refeeding, and to elucidate the underlying molecular mechanisms of adaptation to starvation using biochemical and transcriptomic analyses. Common carp were divided into two groups: a normal control group (NC) fed a commercial diet for 28 days, and a starvation-refeeding group (SR) experiencing 14 days of starvation followed by 14 days of refeeding. The findings indicated that starvation induced oxidative stress, as evidenced by decreased levels of total antioxidant capacity (T-AOC) and glutathione peroxidase (Gpx), alongside increased levels of glutathione-S-transferase (GST), superoxide dismutase (SOD), and malondialdehyde (MDA). Transcriptome analysis revealed that starvation resulted in the differential expression of 2001 genes, including 1259 upregulated and 742 downregulated genes. These differentially expressed genes (DEGs) were significantly enriched in the biosynthesis of amino acids and fatty acid degradation pathways. Furthermore, pathways associated with adaptive responses, including mitophagy, DNA replication, and protein processing in the endoplasmic reticulum (ER), exhibited marked changes following starvation. Additionally, the FoxO and p53 signaling pathways were involved in regulating the physiological adaptations to cope with starvation. Notably, after 14 days of refeeding, most oxidative stress parameters and gene expression profiles in the muscle of common carp returned to normal values. These findings offer new insights into the mechanisms of starvation stress in fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742372"},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of intermittent fasting on behavioral and physiological stress indicators in Pacific abalone during persistent ocean heat waves","authors":"Haowei Cai ,&nbsp;Mo Zhang ,&nbsp;Rui Gao ,&nbsp;Ying Liu ,&nbsp;Xiaolong Gao","doi":"10.1016/j.aquaculture.2025.742367","DOIUrl":"10.1016/j.aquaculture.2025.742367","url":null,"abstract":"<div><div>Global climate change has increased the frequency of marine heat waves, posing a continuous threat to the survival of economically relevant shellfish species. This, in turn, affects the stability of ecological systems and the security of the aquaculture industry. In this study, we investigated the effects of different fasting regimens (GY: fasting at 1-day intervals, GL: fasting at 2-day intervals) on the behavioral and physiological adaptability of Pacific abalone (<em>Haliotis discus hannai</em>), a marine gastropod, particularly in response to recurrent marine heat waves. Our findings revealed that the survival rates of abalone in both the GY and GL groups were significantly higher than those in the unfasted control group (ZC) (<em>P</em> &lt; 0.05). Furthermore, behavioral assessments revealed that the cumulative distance moved, feeding durations, and the percentage of cumulative feeding time relative to movement time were significantly greater in the GL group compared to both the ZC and GY groups (<em>P</em> &lt; 0.05). Sequencing of the 16S rDNA amplicon revealed that the intestinal microbial community of abalone in the GL group harbored a higher abundance of beneficial bacteria (<em>Arenimonas</em>, <em>Pseudolabrys</em>) compared to the GY and ZC groups. In contrast, the potentially harmful bacterium <em>Tenacibaculum</em> was significantly reduced in the GY group relative to the ZC group (<em>P</em> &lt; 0.05). A non-targeted metabolomics analysis identified amino acids and fatty acids, such as Citrulline, Leu-Pro, and L-Glutamate, as differential metabolic markers. KEGG enrichment analysis indicated that the ‘Biosynthesis of amino acids’ pathway was significantly enriched in both the GY and GL groups compared to the ZC group (<em>P</em> &lt; 0.05). Further analysis of 23 amino acids showed that 15 and 20 amino acids, including glycine and serine, were downregulated in the GY and GL groups, respectively, compared to the ZC group. Pearson correlation analysis revealed a positive correlation between the relative abundance of both <em>Arenimonas</em> and <em>Pseudolabrys</em> in the abalone's gut and citrulline content in the GY and GL groups (<em>P</em> &lt; 0.05). These findings suggest that intermittent fasting not only promotes energy redistribution, serving as an effective strategy for abalone to cope with oceanic heat waves, but also provides valuable insights into the behavioral and physiological plasticity of marine shellfish in response to global climate change.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742367"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “QTL mapping reveals predominantly nonadditive genetic architecture of heterotic growth traits in yearling Pacific oysters Crassostrea gigas” [Aquaculture 596 (2025) 741892, February 2025].","authors":"Xiaoshen Yin ,&nbsp;Dennis Hedgecock","doi":"10.1016/j.aquaculture.2025.742356","DOIUrl":"10.1016/j.aquaculture.2025.742356","url":null,"abstract":"","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742356"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Satellite observed rapid inland aquaculture expansion in Jianghan Plain, China from 2016 to 2022” [Aquaculture, Volume 599, 15 April 2025, 742132]","authors":"Chen Wang ,&nbsp;Jinwei Dong ,&nbsp;Yan Zhou ,&nbsp;Yifeng Cui ,&nbsp;Xi Chen ,&nbsp;Yuanyuan Di ,&nbsp;Xiangming Xiao ,&nbsp;Geli Zhang","doi":"10.1016/j.aquaculture.2025.742359","DOIUrl":"10.1016/j.aquaculture.2025.742359","url":null,"abstract":"","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742359"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas13d mediated inhibition of Siniperca chuatsi rhabdovirus infection in fish cells","authors":"Xue-Dong Yu,&nbsp;Qi-Ya Zhang,&nbsp;Fei Ke","doi":"10.1016/j.aquaculture.2025.742355","DOIUrl":"10.1016/j.aquaculture.2025.742355","url":null,"abstract":"<div><div><em>Siniperca chuatsi</em> rhabdovirus (SCRV) is an RNA virus causing lethal disease in Mandarin fish (<em>Siniperca chuatsi</em>). In the present study, the CRISPR/Cas13d system was successfully established and validated in fish cells to suppress SCRV infection. A total of 25 sgRNA spacers were designed to target the genome or mRNA of SCRV, from which five highly effective sgRNAs were selected that consistently inhibited SCRV replication at 12, 24, and 48 h post-infection (hpi). Then, the five sgRNA spacers were combined into a CRISPR array and stable CRISPR/Cas13d-expressing cells were developed to evaluate their inhibitory effect to SCRV infection. The results showed that the Cas13d effector combined with the CRISPR array efficiently suppressed viral amplification within 48 hpi. The CRISPR array also showed a stronger inhibitory effect than that of single sgRNAs. These results demonstrated that the established CRISPR/Cas13d system can effectively target and inhibit SCRV replication and gene expression in fish cells and identified efficient target sequences that could be served as potential antiviral-specific sites. These findings provided new insights into the development of novel strategies for controlling RNA viruses infection including SCRV.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742355"},"PeriodicalIF":3.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}