Aquaculture最新文献

{"title":"A genome-wide association study of growth traits in “Green Pacific hybrid abalone” (Haliotis discus hannai ♀ × H. fulgens ♂)","authors":"Yanyu Yao ,&nbsp;Linyue Wang ,&nbsp;Lu Li ,&nbsp;Xu Lin ,&nbsp;Zhen Zhao ,&nbsp;Hanling Gan ,&nbsp;Yilei Wang ,&nbsp;Ziping Zhang","doi":"10.1016/j.aquaculture.2025.742225","DOIUrl":"10.1016/j.aquaculture.2025.742225","url":null,"abstract":"<div><div>\"Green Pacific hybrid abalone\" is the F1 generation obtained by artificial hybridization of the Pacific abalone <em>Haliotis discus hannai</em> ♀ and green abalone <em>H. fulgens</em>, which has been popular in China's abalone aquaculture industry in recent years due to its advantages of rapid growth. Improving the growth traits of this species contributes to the development of abalone breeding. Therefore, in this study, the genome-wide association study (GWAS) of seven growth traits was conducted in 200 \"Green Pacific hybrid abalone\" with 521,802 SNPs by super GBS sequencing technology. A total of 88 potentially associated SNPs and 28 potentially candidate genes were identified through annotation. Five genes, <em>cdk2</em>, <em>kif13b, pde2a, prss23</em>, and <em>tmem131l</em>, were selected for RT-qPCR verification, and it was inferred that these genes may indirectly regulate the growth of organisms by participating in cell differentiation, growth regulation, material transport and signal transduction in hybrid abalone at different stages of growth and development. The study also examined the relationships between various genotypes of 27 significantly associated SNPs and growth traits. Nine of them had a heterozygous genotype as the dominant genotype, and they all performed significantly better than the homozygous genotype in terms of growth traits. In conclusion, our findings provide an experimental basis for improving the performance of hybrid abalone and accelerating the breeding process, and these markers and genes can also serve as a starting point for exploring the genetic mechanisms underlying growth in hybrid abalone.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742225"},"PeriodicalIF":3.9,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical optimization of Al2(SO4)3 in the coagulation-flocculation-resuspension process for enhanced recovery of spring viraemia of carp virus in freshwater","authors":"Hye Won Kim,&nbsp;Kwang Il Kim","doi":"10.1016/j.aquaculture.2025.742228","DOIUrl":"10.1016/j.aquaculture.2025.742228","url":null,"abstract":"<div><div>Waterborne viruses are present at low concentrations in water and require a concentration process for environmental DNA (eDNA) analysis. The coagulation-flocculation–resuspension (C-F-R) process is an effective method for concentrating viral particles in water. However, its efficiency in freshwater environments is lower than that in seawater, making optimization of the process essential for improving performance. This study optimized the C-F-R process to recover spring viraemia of carp virus (SVCV) from freshwater by comparing two coagulants—Al₂(SO₄)₃ and FeCl₃. The optimal dosage for both coagulants was 2 mg/L, with Al₂(SO₄)₃ exhibiting a more stable performance at various dosages. Al₂(SO₄)₃ achieved effective flocculation within 15 min, whereas FeCl₃ required 60 min. Furthermore, Al-based flocs demonstrated a more efficient resuspension time of 2 h compared to Fe-based flocs. Using the optimized protocols, Al₂(SO₄)₃ presented superior recovery efficiency and consistency across different SVCV concentrations, with overall yields higher for Al than for Fe. To apply the optimized C-F-R process, viral dynamics were analyzed by injecting 10², 10⁴, and 10⁶ SVCV PFU/fish into common carp and monitoring the mortality rate, viral replication, and viral shedding. Viral shedding peaked earlier than viral replication in the liver, indicating that viral shedding was more active in the early to mid-stages of infection than in the later stage. Moreover, the optimized protocols could detect the virus as early as one-day post-infection. The C-F-R process with Al₂(SO₄)₃, therefore, could be applied to enhance the early detection of SVCV and provide a reliable, noninvasive approach for SVCV surveillance.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742228"},"PeriodicalIF":3.9,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143360499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multitrophic aquaculture improves the production performance of Amazonian fishes in nursery phase","authors":"Jesaías Costa ,&nbsp;Ronãn Alves de Freitas ,&nbsp;Manoel Pio Nonato Neto ,&nbsp;Ana Paula Nunes de Sena ,&nbsp;Diany Bastos Bezerra ,&nbsp;Carolina Flores Nascimento ,&nbsp;Joana Alcina Silvestre Natividade ,&nbsp;Thyssia Bomfim Araujo da Silva","doi":"10.1016/j.aquaculture.2025.742240","DOIUrl":"10.1016/j.aquaculture.2025.742240","url":null,"abstract":"<div><div>Integrated Aquaculture (IA) is a promising production model for the Amazon region, which involves the compatibility and complementarity of fish species, with better use of space and the use of artificial and natural food. This study aimed to evaluate the production of tambaqui (<em>Colossoma macropomum,</em> initial weight 2.22 ± 0.51 g), matrinxã (<em>Brycon amazonicum</em>, initial weight 2.54 ± 0.81 g) and curimbatá (<em>Prochilodus sp.</em>, initial weight 1.64 ± 0.53 g) in integrated and monoculture systems. The experiment was carried out in a completely randomized design for 16 weeks. We stocked 120 fish per pond (46.62 m²) in the following treatments:1) Mono-T, 100 % tambaqui; 2) Mono-M, 100 % matrinxã; 3) Mono-C, 100 % curimbatá; 4) IA-TC, 70 % tambaqui and 30 % curimbatá; 5) IA -MC, 70 % matrinxã and 30 % curimbatá; 6) IA -TM, 50 % tambaqui and 50 % matrinxã; and 7) IA -TMC, 40 % matrinxã, 40 % tambaqui and 20 % curimbatá. The water quality, the production performance of the species and the system were evaluated. The IA systems (IA-TC, IA-MC and IA-TMC) with curimbatá showed higher dissolved oxygen values (&gt; 5 mg.L⁻¹), lower ammonia peaks (&lt;4 mg.L⁻¹), higher turbidity (&gt;15 NTU) and lower nitrite values (&lt;0.04 mg.L⁻¹). The matrinxã had greater growth (Final weight 253.63 ± 27.58 g) when raised in integrated with tambaqui and curimbatá (IA-TMC) (<em>P</em> &lt; 0.05). Tambaqui monoculture presented the best feed conversions (0.80 ± 0.11, <em>P</em> &lt; 0.05). In general, the IA system allowed a higher production performance of tambaqui, matrinxã and curimbatá when compared to the monoculture system (<em>P</em> &lt; 0.05), but the tambaqui monoculture had the best productive performance in the evaluated systems (<em>P</em> &lt; 0.05).</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742240"},"PeriodicalIF":3.9,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143360252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying current trends in the environmental impacts linked to fishmeal and fish oil production in Peru","authors":"Alejandro Deville ,&nbsp;Ian Vázquez-Rowe ,&nbsp;Angel Avadí ,&nbsp;Fernando Miranda ,&nbsp;Ramzy Kahhat","doi":"10.1016/j.aquaculture.2025.742239","DOIUrl":"10.1016/j.aquaculture.2025.742239","url":null,"abstract":"<div><div>The anchoveta (<em>Engraulis ringens</em>) fishery in Peru, which is almost entirely devoted to the production of fishmeal and fish oil, is one of the largest fisheries in the world. It is volatile in terms of fishing stock availability, and the fishmeal industry has been subject to technological changes to upgrade its efficiency and reduce costs to maintain its competitiveness. The objective of this study is to apply the Life Cycle Assessment methodology to the production and exportation of fishmeal and fish oil products related to a relevant producer in Peru, representing 10 % of national production. A set of 169 vessels targeting <em>E. ringens</em> were inventoried, 88 % of which are owned by third parties, and four factories belonging to the company were assessed for the years 2019 and 2021. Ecoinvent was the selected database to support the life cycle inventory, and ReCiPe 2016 and IPCC 2021 were the methods applied to compute the environmental impacts. The results show that fuel combustion in fishmeal and fish oil production was the dominating activity in most of the impact categories analyzed. In terms of greenhouse gas emissions, it was found that, on average, approximately 320 kg CO₂eq and 4430 kg CO₂eq are emitted due to the production of 1 t of fishmeal and 1 t of fish oil, respectively, when an energy allocation is followed. The fishery accounted for ca. 45 % of greenhouse gas emissions and dominated most of the impact categories, showing greater influence of the fishing stage than in previous studies. The reasons behind are linked to the combined influence of improvements in the energy matrix of the plants, by prioritizing natural gas over diesel and residual fuel oils, and a slightly higher fuel use intensity of the fishing fleet. <em>E. ringens</em> quality was found to be an important parameter, as low protein or fat yields translate into substantially higher impacts. Finally, although Peruvian fishmeal and fish oil remain as one of the lowest environmental footprint products among animal feed, future work is needed to understand the effects that climate change and El Niño-Southern Oscillation events have on this industry.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742239"},"PeriodicalIF":3.9,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143359838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the sex-linked region of Siniperca scherzeri and development of sex-specific markers","authors":"Yang Li ,&nbsp;Yu Cui ,&nbsp;Xiaojun Ye ,&nbsp;Maoyuan Wang ,&nbsp;Hao Xu ,&nbsp;Yannian Wu ,&nbsp;Jian Jin ,&nbsp;Honggui Huang ,&nbsp;Mingyong Lai ,&nbsp;Zhiyong Wang ,&nbsp;Dongling Zhang","doi":"10.1016/j.aquaculture.2025.742231","DOIUrl":"10.1016/j.aquaculture.2025.742231","url":null,"abstract":"<div><div>Spotted mandarin fish <em>Siniperca scherzeri</em>, as a significant freshwater aquaculture species in China, displays pronounced sexual dimorphism. Adult female <em>S. scherzeri</em> typically weigh over 10–30 % more than their male counterparts. Nonetheless, the external morphological differences between females and males are not readily noticeable, especially during the juvenile stage, with challenging to precisely distinguish and confirm their morphological sex using traditional methods. Moreover, the genetic mechanisms underlying sex determination in <em>S. scherzeri</em> are unclear. Hence, it is crucial to investigate the genetic basis of sex in <em>S. scherzeri</em> in-depth and develop corresponding molecular markers. Such advancements are essential for the future implementation of sex-controlled breeding program. In this study, whole-genome resequencing was conducted on 50 <em>S. scherzeri</em> (25 males and 25 females), followed by a genome-wide association analysis (GWAS) integrated with an Fst analysis. For the first time, chromosome 14 was identified as the sex chromosome in <em>S. scherzeri</em> and a 0.44 Mb region (19.34 Mb–19.78 Mb) was confirmed as the sex-linked region. Within this region, 19 SNPs and 4 InDels related to gender dimorphic were screened. From these, two InDels were selected to develop one male-specific marker and one female-specific marker, both achieving a 100 % accuracy rate in sex identification. Furthermore, the developed sex markers and sex-linked SNPs confirmed that <em>S. scherzeri</em> exhibits an XX/XY male heterogametic reproductive system. These findings provide significant propositions and essential tools for elucidating the molecular mechanisms of sex determination and differentiation in <em>S. scherzeri</em>, and for developing sex-controlled breeding programs as well.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742231"},"PeriodicalIF":3.9,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143359835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of creatine treatment at low temperature on the development of ovarian follicles before sexual maturity in Pelodiscus sinensis","authors":"Guoyin Zhang ,&nbsp;Yijing Liu ,&nbsp;Yuzheng Li ,&nbsp;Junbo Tian ,&nbsp;Yuanjie Zhu ,&nbsp;Guoying Qian ,&nbsp;Zhongfa Chen ,&nbsp;Caiyan Li ,&nbsp;Wei Wang","doi":"10.1016/j.aquaculture.2025.742242","DOIUrl":"10.1016/j.aquaculture.2025.742242","url":null,"abstract":"<div><div>As a nitrogen-containing organic acid, creatine can stabilize cellular adenosine triphosphate (ATP) levels and promote growth. For reptiles with hibernation habits, creatine has a special role in regulating energy metabolism under low temperature conditions. This study was conducted to investigate the effect of creatine on the ovarian development of <em>Pelodiscus sinensis</em> (<em>P. sinensis</em>) under low temperature. After 8 weeks of creatine treatment, in the ovaries of the low concentration creatine treatment group, follicle diameter, yolk granule accumulation, creatine kinase (CK) expression, and glycogen content were all significantly increased (<em>p</em> &lt; 0.05), while the lactic acid content significantly decreased (<em>p</em> &lt; 0.05). However, there were no significant differences in ovarian weight and of number of follicles between the high concentration treatment group and the GPA treatment group when compared to the control group (<em>p</em> &gt; 0.05). Therefore, the metabolism of the ovary at low temperatures is of great significance in growth and development. It is suggested that creatine may play an important role in improving ovarian development under low temperature conditions for <em>P. sinensis.</em> This study also provides a theoretical basis to enhance the female fertility in other hibernating animals.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742242"},"PeriodicalIF":3.9,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143338957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth, appetite, and mineral deposition in rainbow trout reared in fresh- or seawater under different CO2 regimes","authors":"Tilo Pfalzgraff ,&nbsp;Helene Volkoff ,&nbsp;Tine Moesgaard Iburg ,&nbsp;Heidi Amlund ,&nbsp;Peter Vilhelm Skov","doi":"10.1016/j.aquaculture.2025.742234","DOIUrl":"10.1016/j.aquaculture.2025.742234","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Despite degassing efforts in recirculating aquaculture systems (RAS) and short hydraulic retention time in rearing units, carbon dioxide (CO&lt;sub&gt;2&lt;/sub&gt;) concentrations reach a hypercapnic steady state. Furthermore, as CO&lt;sub&gt;2&lt;/sub&gt; excretion is correlated with the oxygen consumption of fish and bacteria, CO&lt;sub&gt;2&lt;/sub&gt; levels in RAS may undergo fluctuations, of greater or smaller magnitude, depending on systems design and operation. Experimental approaches to assess the effects of CO&lt;sub&gt;2&lt;/sub&gt; on fish usually subject fish to constant CO&lt;sub&gt;2&lt;/sub&gt; concentrations, which might not reflect rearing conditions on an industrial production scale. Here, we compare the effects of oscillating against constantly elevated CO&lt;sub&gt;2&lt;/sub&gt; levels on the appetite, growth, feed utilization, and mineral deposition in three separate growth trials. Two trials were conducted in freshwater (FW) and one in seawater (SW). In each trial, rainbow trout (&lt;em&gt;Oncorhynchus mykiss&lt;/em&gt;) were subjected to four different CO&lt;sub&gt;2&lt;/sub&gt; treatments: either constant levels of 10 mg/L CO&lt;sub&gt;2&lt;/sub&gt; (&lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; = 3.86 mmHg in FW / 4.57 mmHg in SW), 25 mg/L CO&lt;sub&gt;2&lt;/sub&gt; (&lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; = 9.65 mmHg in FW / 11.42 mmHg in SW), fluctuating between these two concentrations over 24 h, or normocapnic control conditions of ≤3 mg/L CO&lt;sub&gt;2&lt;/sub&gt; (&lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; ≤ 1.16 mmHg in FW / 1.37 mmHg in SW) for at least 5 weeks on fixed daily rations of 1.3 % of the tank biomass. In one of the freshwater trials, the diet contained high levels of phosphorus (1.8 %) to assess if elevated dietary phosphorus concentrations promoted mineralization in kidney tissues (nephrocalcinosis) under hypercapnic conditions. In both freshwater trials, fish at all CO&lt;sub&gt;2&lt;/sub&gt; levels accepted the offered feed, while in seawater daily feed intake was reduced by 35 % at exposure to 25 mg/L CO&lt;sub&gt;2&lt;/sub&gt; (&lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; = 11.42 mmHg). Despite accepting the full, albeit restricted ration, fish reared in freshwater showed that CO&lt;sub&gt;2&lt;/sub&gt; affected appetite, evidenced by changes in mRNA expression of appetite-regulating peptides in the hypothalamus and liver of the fish. This finding was confirmed by a maximum voluntary feed intake test, showing that fish consumed less food at higher CO&lt;sub&gt;2&lt;/sub&gt; concentrations. Despite consuming similar daily ration sizes, the specific growth rate (SGR) and feed conversion ratio (FCR) were significantly affected in the 25 mg/L freshwater group (&lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; = 9.65 mmHg) but not at 10 mg/L (&lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; = 3.86 mmHg) or when oscillating between those concentrations. In the seawater trial, however, SGR and FCR of the trout were already significantly reduced at 10 mg/L of dissolved CO&lt;sub&gt;2&lt;/sub&gt; (&lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; = 4.57 mmHg) compared to the control group, but even more so in the 25 mg/L CO&lt;sub&gt;2&lt;/sub&gt; group (&lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; = 11.42 mmHg). While the CO&lt;sub&gt;2&lt;/sub&gt; regimes applied in t","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742234"},"PeriodicalIF":3.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143359841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing total factor productivity change in the aquaculture industry in China: A Färe-Primont productivity index and panel quantile regression","authors":"Yujie Li ,&nbsp;Kim Huat Goh ,&nbsp;Ee Shiang Lim ,&nbsp;Ghee-Thean Lim","doi":"10.1016/j.aquaculture.2025.742230","DOIUrl":"10.1016/j.aquaculture.2025.742230","url":null,"abstract":"<div><div>Given the limited resources and growing demand for aquatic products, China's aquaculture industry must increase its output. Improving the total factor productivity (TFP) change is an important step toward long-term sustainable development of the aquaculture industry. The study aims to examine the productivity change, particularly TFP change and its components, and to identify the factors influencing TFP change in China's aquaculture industry during 2010–2018. The Färe-Primont productivity index (FPI) is employed to assess the TFP change in the aquaculture industry. Subsequently, a novel introduced model - fixed effects panel quantile regression via the method of moments is used to analyze the heterogeneous effects of the influencing factors, namely, capture fishery ratio, extension workers, disaster losses, and real gross domestic product (GDP), on TFP change and efficiency change (ECH). The results show that the TFP grew by an average of 1.35 % during the period studied, driven mainly by a 3.63 % improvement in ECH, despite a decline of 1.86 % in technical change. Notably, inland provinces such as Chongqing, Shaanxi, Guizhou, and Yunnan predominantly exhibit higher TFP change than the coastal provinces. The results also demonstrated that socio-economic factors exerted differential impacts on the TFP change and the ECH of the aquaculture industry. The real GDP has positively influenced TFP change, exhibiting a downward trend across quantiles whereas the extension workers positively contribute to TFP change only at lower quantiles (0.10–0.50). Moreover, the disaster losses exert a positive impact on TFP change only at higher quantiles (0.50–0.90). The real GDP significantly improved ECH at all quantiles, while the disaster losses have a negative impact on ECH across all the quantiles except for the 0.10 quantile level. These findings highlight several policy implications. First, the aquaculture industry should train more technical workforce and facilitate cross-departmental rotations for these personnel. Second, less economically developed provinces need to adopt a better scientific management practice and improve the allocation of resources in aquaculture operations. Lastly, regions prone to natural disasters should develop more robust systems for disaster preparedness and response systems to minimize productivity losses.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742230"},"PeriodicalIF":3.9,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yellow catfish (Pelteobagrus fulvidraco) lacking of germ cells exhibited gonadal sexual dimorphism","authors":"Huan Ye ,&nbsp;Yanling Hou ,&nbsp;Ling Huang ,&nbsp;Huamei Yue ,&nbsp;Ziling Qu ,&nbsp;Rui Ruan ,&nbsp;Chuangju Li","doi":"10.1016/j.aquaculture.2025.742227","DOIUrl":"10.1016/j.aquaculture.2025.742227","url":null,"abstract":"<div><div>Yellow catfish (<em>Pelteobagrus fulvidraco</em>) is a valuable freshwater aquaculture species in China, characterized by an XX/XY sex determination system and pronounced sexual dimorphism in growth. Primordial germ cells (PGCs), progenitors of germ cells, play an essential roles in gonadal sex differentiation and development. In order to reveal the function of germ cells in the sexual differentiation of yellow catfish, PGCs were ablated during embryogenesis through the morpholino-mediated knockdown of the <em>dead end</em> (<em>dnd</em>) gene. The PGCs-depleted gonads exhibited two distinct histological types: one characterized by an ovarian cavity structure (type I gonad) and the other by seminiferous lobule-like structures (type II gonad). Additionally, the expression profiles of germ cell-specific and gonadal somatic cell-related genes were analyzed in the 12-month-old wild-type (WT) fish and <em>dnd</em> morphants. Germ cell-specific genes, <em>dnd</em>, <em>vasa</em>, and <em>dmc1</em>, were identified in the gonads of WT fish but were absent in the gonads of morphants. Genes associated with ovarian somatic cells, <em>cyp19a1</em> and <em>foxl2</em>, were primarily found in the ovaries of WT fish and in type I gonads. Conversely, testicular somatic cell-related genes, <em>amh</em>, <em>cyp11b</em>, and <em>dmrt1</em>, exhibited predominant or elevated expression levels in the testes of WT fish and in type II gonads. These findings suggested that the differentiation of gonadal somatic cells in yellow catfish occurs independently of germ cells. Our study holds significant importance for advancing the understanding of sex differentiation in yellow catfish, and making an ideal candidate recipient for germline stem cell transplantation within the Bagridae family.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742227"},"PeriodicalIF":3.9,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143338959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and genomic characterization of multi-drug resistant Morganella morganii strain from Chinese Soft-Shell Turtle Pelodiscus sinensis","authors":"Rong-Hua Wang ,&nbsp;Meng-Lu Yan ,&nbsp;Qing Tan ,&nbsp;Jin-Long Wang ,&nbsp;Yu-Shuang Luo ,&nbsp;Zhong-Yuan Chen","doi":"10.1016/j.aquaculture.2025.742229","DOIUrl":"10.1016/j.aquaculture.2025.742229","url":null,"abstract":"<div><div>An outbreak of granulomatous disease in the visceral organs was observed at a Chinese Soft-Shell Turtle (CSST, <em>Pelodiscus sinensis</em>) aquaculture facility in Changde, Hunan Province, China. The pathology was primarily characterized by the presence of opalescent nodules in vital organs such as the liver, spleen, and heart. The study involved the isolation and identification of the pathogen responsible for the disease, and analyzed its pathogenicity, drug resistance, and genomic characteristics. Results showed the isolation of a dominant strain, HD0531g, from the liver and spleen of affected CSSTs, identified as <em>Morganella morganii</em> through physiological and biochemical tests. Phylogenetic analysis based on 16S rRNA sequences revealed that HD0531g clusters with <em>M. morganii</em> strains M11, JCM1672, and NBRC3848. Experimental infection models demonstrated that the median lethal dose (LD₅₀) of strain HD0531g in CSSTs was 1.05 × 10⁶ CFU/mL. Antimicrobial susceptibility testing revealed pronounced antibiotic resistance in HD0531g, except for its susceptibility to gentamicin. Pathological observation revealed numerous nodular lesions in the liver and spleen of CSSTs, accompanied by severe hemorrhage and inflammation. Whole-genome sequencing revealed that the HD0531g strain has a total genomic length of about 3.90 Mb, including a 2311 bp plasmid and encoding 3653 genes. Phylogenetic analysis of the entire genome placed HD0531g in the same cluster as the <em>M. morganii</em> strain NBRC3848. A virulence factor database query identified the presence of 136 virulence genes in strain HD0531g, representing 47 species across 15 categories and encoding an array of pathogenic proteins including secretion systems, adhesins, and lipooligosaccharides (LOS). Drug resistance gene mapping indicated that HD0531g contains 16 distinct antibiotic resistance genes, most of which are related to antibiotic inactivation and antibiotic efflux. In summary, this study isolated a highly pathogenic, multi-drug resistant strain of <em>M. morganii</em> from CSSTs affected by visceral white spot disease. It detailed the distribution of antibiotic resistance and virulence genes in the strain, offering valuable insights for treatment and the rational use of drugs in HD0531g infections in CSSTs.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"600 ","pages":"Article 742229"},"PeriodicalIF":3.9,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143338967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}