Aquaculture最新文献

{"title":"Food availability, but not tidal emersion, influences the combined effects of ocean acidification and warming on oyster physiological performance","authors":"Coline Caillon ,&nbsp;Elodie Fleury ,&nbsp;Carole Di Poi ,&nbsp;Frédéric Gazeau ,&nbsp;Fabrice Pernet","doi":"10.1016/j.aquaculture.2025.742459","DOIUrl":"10.1016/j.aquaculture.2025.742459","url":null,"abstract":"<div><div>Many studies on the effects of ocean acidification and warming (OAW) in intertidal mollusks overlook critical factors like tidal emersion and food availability, both of which can shape organisms' responses. Experiments on intertidal bivalves often use constant immersion and abundant food, which likely underestimate global change impacts and underscore the need for more realistic experiments mimicking natural ecosystems. This study investigated the physiological responses of juvenile Pacific oyster <em>Crassostrea gigas</em> exposed for 81 days to current and OAW conditions (+3 °C, −0.3 pH units) under two tidal treatments (0 vs. 30 % emersion) and two food levels (ad libitum vs. limited). We measured growth, reproduction, food ingestion, respiration, and biochemical traits like energy reserves and membrane fatty acids. At the experiment's end, oysters were challenged with a viral disease to assess the physiological cost of acclimation and potential trade-offs. Results showed improved oyster physiological performance under OAW with high food level. Nevertheless, food availability emerged as the predominant factor in oyster performance, limiting growth, reproduction, and energy reserves, while increasing oxygen consumption and disease susceptibility. Food deprivation attenuated the beneficial effects of OAW through antagonistic interaction, suggesting physiologically weakened oysters may struggle to adapt to environmental hazards. Finally, tidal treatment had no significant effect, implying that oysters possess physiological compensatory mechanisms, particularly in food acquisition, enabling them to meet nutritional needs during immersion periods. This study provides valuable insights for designing global climate change experiments that align with ecological realism and improves our understanding of the acclimation potential in bivalves facing rapid ocean changes.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742459"},"PeriodicalIF":3.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic parameters and genome-wide association studies of image-derived cardiac traits in Tasmanian Atlantic salmon (Salmo salar)","authors":"Timothy D.W. Luke,&nbsp;Richard S. Taylor,&nbsp;Wagdy Mekkawy,&nbsp;Roberto Carvalheiro,&nbsp;Brad S. Evans,&nbsp;Curtis E. Lind","doi":"10.1016/j.aquaculture.2025.742436","DOIUrl":"10.1016/j.aquaculture.2025.742436","url":null,"abstract":"<div><div>Cardiac morphology, including ventricle shape and epicardial fat coverage, differs significantly between farmed and wild Atlantic salmon (<em>Salmo salar</em>) and is associated with health and performance. While environmental and dietary effects are increasingly understood, the genetic basis of these traits remains less explored in part due to the difficulty in obtaining phenotypes. We developed an image-based phenotype for epicardial fat coverage, achieving a strong correlation with ground truth measurements obtained using computer tomography (<em>R</em> = 0.87). An algorithm to measure ventricle height and width from images was also developed. Using data collected from 1792 individuals from the Salmon Enterprises of Tasmania (SALTAS) selective breeding program, we performed quantitative genetic and genomic analyses of these traits, along with heart weight and cardio somatic index (CSI). Estimated heritabilities were 0.46 ± 0.06 for epicardial fat coverage, 0.33 ± 0.05 for heart weight, 0.30 ± 0.05 for CSI, and 0.29 ± 0.05 for ventricle shape. Genetic correlations between cardiac traits were weak to moderate (0.04–0.51), as were all correlations between cardiac and production traits (0.00–0.46) except for that between heart weight and harvest weight (0.85 ± 0.04). Genome wide association studies demonstrated that all cardiac traits are highly polygenic and identified candidate genes linked to cardiac function, cardiovascular development, lipid metabolism, and obesity. These results highlight the potential of image-based phenotyping for selective breeding and suggest genetic improvement or monitoring of cardiac morphology and health is achievable. However, further studies are needed to validate these findings and better understand associations between cardiac traits, health, and performance.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742436"},"PeriodicalIF":3.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic parameters of resistance to acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus and their genetic correlations with growth traits in an Ecuadorian Penaeus vannamei population","authors":"Marina Martínez Soler ,&nbsp;Hyun Suk Shin ,&nbsp;Álvaro Lorenzo-Felipe ,&nbsp;María Jesús Zamorano Serrano ,&nbsp;Pedro Luis Castro ,&nbsp;Laura Cristina Pachón Mesa ,&nbsp;Jenny Antonia Rodríguez ,&nbsp;Cecilia Tomalá ,&nbsp;Stanislaus Sonnenholzner ,&nbsp;Roberto Carvalheiro ,&nbsp;Wagdy Mekkawy ,&nbsp;Luis Fernando Aranguren ,&nbsp;Eduardo Reyes Abad ,&nbsp;Juan Manuel Afonso López","doi":"10.1016/j.aquaculture.2025.742458","DOIUrl":"10.1016/j.aquaculture.2025.742458","url":null,"abstract":"<div><div>Infections with strains of <em>Vibrio parahaemolyticus</em> causing acute hepatopancreatic necrosis disease (VP_AHPND) in <em>Penaeus vannamei</em> have not yet caused high levels of mortality in Ecuador like those reported in Asia; however, target animals resistant to VP_AHPND are a goal for industrial farmers. The implementation of breeding programs to produce fast-growing and disease-resistant shrimp would be a key to addressing the expected situation. The main objective of this study was to estimate heritabilities and genetic correlations for VP_AHPND resistance and growth traits in an Ecuadorian population cultured under industrial conditions. A total of 3345 animals from 155 sibling families of the PMG-BIOGEMAR© genetic breeding program were individually tagged, measured for initial length and weight (only for Test-1), and tested for VP_AHPND in two trials: Test-1 at a concentration of 2 × 10⁵ CFU/ml and infection by immersion and Test-2 at a concentration of 2 × 10⁸ CFU/g and by oral infection. After 72 h, all shrimp were analysed for final weight and length, survival, and infection levels by Ct values (qPCR) of the <em>pirA</em> and <em>pirB</em> genes. Genetic parameters of growth and resistance traits were obtained using two statistical models: Linear and Threshold models. Heritabilities were medium (0.16–0.31) for growth and low (&lt;0.09) for infection-level traits, by both methods and in both tests. In the case of survival, the heritability was low using the Linear model (0.04), and medium (0.22 and 0.26) with the Threshold model, in Test-1 and Test-2, respectively. However, the genetic correlations found between growth and survival traits were high and positive (&gt;0.55) with both methodologies in Test-1 and low-medium and positive in Test-2 using Threshold model. The results suggest that genetic selection for growth in <em>P. vannamei</em> has a positive effect on resistance to AHPND. Furthermore, the genetic selection for growth over time in this Ecuadorian population may have induced possible resistance or tolerance to the disease.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742458"},"PeriodicalIF":3.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of heat tolerance and molecular adaptations in Venerupis aspera and Ruditapes philippinarum","authors":"Shuang Li ,&nbsp;Yue Tan ,&nbsp;Yumei Tang ,&nbsp;Teng Liang ,&nbsp;Yunzhi Li ,&nbsp;Xiwu Yan ,&nbsp;Zhongming Huo","doi":"10.1016/j.aquaculture.2025.742451","DOIUrl":"10.1016/j.aquaculture.2025.742451","url":null,"abstract":"<div><div>The impact of climate change on marine organisms is increasingly significant, highlighting the importance of understanding their heat tolerance mechanisms to predict and respond to future environmental changes. This study compares the physiological and molecular responses of the closely related clam species <em>Venerupis aspera</em> and <em>Ruditapes philippinarum</em> under heat stress conditions, revealing adaptive mechanisms of heat tolerance. Energy metabolism measurements and enzyme activity analyses revealed that <em>V. aspera</em> exhibits significantly higher heat tolerance than <em>R. philippinarum</em> in heat stress. <em>V. aspera</em> showed greater metabolic flexibility and elevated enzyme activities, enhancing physiological adaptability. Furthermore, in-depth transcriptomic analysis of the gene expression patterns under heat stress demonstrated that <em>V. aspera</em> up-regulated genes associated with heat shock proteins, antioxidant enzymes, and energy metabolism. In contrast, <em>R. philippinarum</em> exhibited fewer changes in the expression of these critical genes. This study provides original insights into the heat tolerance differences between <em>V. aspera</em> and <em>R. philippinarum</em>, elucidating their underlying molecular mechanisms. The dual regulation observed in <em>V. aspera</em>, achieved through metabolic adjustments and gene expression under heat stress conditions, contributes valuable knowledge to our understanding of biological adaptation to climate change. The findings have implications for marine ecological conservation strategies and resource management.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742451"},"PeriodicalIF":3.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance, swimming capacity, and fillet quality in rainbow trout fed a transgene-derived omega-3 and carotenoid-enriched oil","authors":"Pauline Wischhusen ,&nbsp;Angelico Madaro ,&nbsp;Malthe Hvas ,&nbsp;Richard Broughton ,&nbsp;Lihua Han ,&nbsp;Karla Fernandez Quiroz ,&nbsp;Kasidis Chaiyasut ,&nbsp;Akhil Gupta ,&nbsp;Rolf Erik Olsen ,&nbsp;Stéphanie Fontagné-Dicharry ,&nbsp;Johnathan A. Napier ,&nbsp;Mónica B. Betancor","doi":"10.1016/j.aquaculture.2025.742453","DOIUrl":"10.1016/j.aquaculture.2025.742453","url":null,"abstract":"<div><div>A new oil from transgenic <em>Camelina sativa</em> was evaluated for its potential to serve as a primary source for eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and astaxanthin in feed for rainbow trout (<em>Oncorhynchus mykiss</em>). Experimental diets were formulated containing either the transgene-derived oil (CAM) or a blend of fish and vegetable oils (CTL). The two diets were given to quadruplicate tanks of 30 fish (body weight 294 ± 32 g) for 10 weeks. Some of the fish were exercised in a swim tunnel prior to sampling. At sampling, muscle tissue was collected for fillet quality assessment and plasma to measure oxidative stress markers. Dietary treatment had no significant impact on final body weight. The fatty acid profile of rainbow trout fillets reflected that of the experimental diets with higher levels of linoleic acid, arachidonic acid, EPA and DHA in fish fed CAM. Levels of corresponding lipid inflammatory mediators, except for those derived from DHA, were pre-exercise elevated in plasma of fish fed CAM. Similarly, dietary ketocarotenoid levels were reflected in the fillet of fish with lower astaxanthin in fish fed CAM compared to CTL, which agreed with differences in fillet color. Dietary treatment had no significant impact on swimming performance. Plasma cortisol and 8-isoprostane were elevated post-exercise, irrespective of dietary treatment. Feeding the transgene-derived oil supported normal growth and increased fillet EPA and DHA levels above standard. A comparable swimming capacity and stress response suggests that the inclusion of the transgenic camelina oil did not impair fish welfare.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742453"},"PeriodicalIF":3.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of feed satiation on energy metabolism in the mussel Mytilus coruscus under ocean acidification and warming and possible hormonal regulation","authors":"Bole Wang ,&nbsp;Fahim Ullah Khan ,&nbsp;Menghong Hu ,&nbsp;Youji Wang","doi":"10.1016/j.aquaculture.2025.742440","DOIUrl":"10.1016/j.aquaculture.2025.742440","url":null,"abstract":"<div><div>Over the past several decades, it is thought that human activities have been a significant factor in bringing about increased atmospheric carbon dioxide concentrations, thereby contributing to elevated ocean acidification and warming. Due to their sessile nature, marine bivalves are considered to be particularly vulnerable to such alterations of the marine environment. Much remains unknown concerning the underlying strategies by which marine bivalves modify energy demand under such conditions. In addition, whether feed availability has an effect and plays a role in this respect is unclear. In the present study, we attempted to address these questions by investigating metabolism and energy pathways in the mussel <em>Mytilus coruscus</em>. Warming, acidification, and food shortage have significantly impacted metabolism, energy pathways, circadian rhythms, hormone activity, and gene expression related to metabolism and circadian rhythms in mussels, leading to energy limitations. Thermal tolerance data indicate that <em>M. coruscus</em> has substantial adaptive capacity to environmental stressors. Bivalves can regulate energy production pathways by modulating serotonin and triiodothyronine hormones, and adequate food availability enhances this regulation. Our findings suggest that ocean warming and acidification synergistically alter energy metabolism in mussels, causing energy limitations, and that food availability is crucial for maintaining mussel condition.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742440"},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new type of triploid fish derived from female Carassius auratus red var. × male Gobiocypris rarus","authors":"Fanglei Liu ,&nbsp;Ziyi Huang ,&nbsp;Xuanyi Zhang,&nbsp;Bei Li,&nbsp;Lujiao Duan,&nbsp;Jianming Yu,&nbsp;Qizhi Liu,&nbsp;Siyang Huang,&nbsp;Hongwen Liu,&nbsp;Qiuli Liang,&nbsp;Qingfeng Liu,&nbsp;Shaojun Liu","doi":"10.1016/j.aquaculture.2025.742442","DOIUrl":"10.1016/j.aquaculture.2025.742442","url":null,"abstract":"<div><div>Distant hybridization can combine the genomes of the parental species, leading to changes in both genotypes and phenotypes in the hybrids, often accompanied by the appearance of polyploid progeny. In this study, we obtained an allotriploid hybrid (RR, 3n = 125) through inter-subfamily hybridization between female red crucian carp (<em>Carassius auratus</em> red var., RCC, 2n = 100) and male rare gudgeon (<em>Gobiocypris rarus</em>, RG, 2n = 50). We investigated the fertilization rate, hatching rate, morphological traits, chromosome number, DNA content, 5S rDNA, mitochondrial DNA (mtDNA), growth rate and muscle fibers in RR. The results indicated that RR is an allotriploid fish with 125 chromosomes. RR exhibited a fertilization rate of 70.30 %, a hatching rate of 52.70 %, and displayed morphological traits that were intermediate between those of its parents. The 5S rDNA of RR inherited specific bands from both parents, with recombination and mutation. Through sequence alignment and the NJ evolutionary tree analysis, the mtDNA of RR showed a higher similarity to that of RCC, with some partial base insertions observed, indicating that the mitochondria of RR follows maternal inheritance. At 12 months of age, the mean weight of RR was significantly increased by 18.43 % compared to RCC. Furthermore, the total area and mean area of muscle fiber of RR increased significantly than those of RCC, indicating muscle hypertrophy and hyperplasia. This study provided a new approach to create allotriploid fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742442"},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockout of the fih gene by CRISPR/Cas9 enhances the hypoxia tolerance in grass carp (Ctenopharyngodon idella)","authors":"Huimei Zhang ,&nbsp;Ziquan Yang ,&nbsp;Jianjun Fu ,&nbsp;Ziming Yuan ,&nbsp;Jie Chen ,&nbsp;Rengao Shen ,&nbsp;Guodong Zheng ,&nbsp;Shuming Zou ,&nbsp;Zaijie Dong","doi":"10.1016/j.aquaculture.2025.742452","DOIUrl":"10.1016/j.aquaculture.2025.742452","url":null,"abstract":"<div><div>Grass carp (<em>Ctenopharyngodon idella</em>), a key freshwater fish species in China, is threatened by hypoxia in aquaculture due to high stocking densities and climate change. Factor inhibiting hypoxia-inducible factor (FIH), an asparagine hydroxylase that represses the expression of hypoxia-inducible factor (HIF), inhibits the transcription of hypoxia-responsive genes by blocking interactions between HIF and the coactivators CBP and p300. In this study, we used CRISPR-Cas9 genome editing to generate <em>fih</em>-deficient heterozygotes of grass carp. Our study demonstrates that individuals with a deletion of the <em>fih</em> gene exhibit significantly enhanced hypoxia tolerance compared to wild-type grass carp, as evidenced by the critical oxygen tension at which equilibrium is lost (LOE_crit). Furthermore, although the hypoxia tolerance of grass carp has increased, <em>fih</em> gene knockout did not have a significant impact on their growth and development. We performed real-time quantitative PCR and TUNEL staining to assess hypoxic responses in <em>fih</em>-deficient grass carp, and accordingly found that compared with the wild grass carp, certain hypoxia-responsive genes, including <em>hif1α</em>, <em>phd3</em>, and g<em>lut1</em>, were upregulated in <em>fih</em>-deficient individuals. Additionally, we established that, under hypoxic conditions, levels of apoptosis in the gills and brains of <em>fih</em>-deficient fish were significantly lower than those in their wild counterparts. These findings indicate that <em>fih</em> plays an essential role in hypoxia tolerance of grass carp and providing candidate genes for the genetic breeding of hypoxia-tolerant varieties.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742452"},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A TaqMan probe-based RT-qPCR for the detection of brine shrimp reovirus 1","authors":"Xuan Li ,&nbsp;Guohao Wang ,&nbsp;Haoyu Lou ,&nbsp;Chengyan Zhou ,&nbsp;Xiangmin Meng ,&nbsp;Xinping Wang ,&nbsp;Xuan Dong","doi":"10.1016/j.aquaculture.2025.742444","DOIUrl":"10.1016/j.aquaculture.2025.742444","url":null,"abstract":"<div><div>Brine shrimp (<em>Artemia</em>) are widely utilized as a vital live feed in the aquaculture industry. Recently, we found a novel reovirus - brine shrimp reovirus 1 (BSR1) in brine shrimp. Considering the potential impact of reovirus on the industry, it is necessary to develop a highly sensitive and specific detection method in advance for identifying BSR1. In this study, we established a real-time quantitative reverse transcription PCR (RT-qPCR) method based on TaqMan probe. The detection limit of this method is as low as 4.4 × 10¹ copies/reaction. The standard curve between 4.4 × 10¹ to 4.4 × 10⁹ copies/reaction showed a high correlation coefficient (R² = 0.997). This newly developed method was used to screen for BSR1 in brine shrimp cyst samples from Africa, Asia, Europe, Oceania, South America and North America, and the results showed that BSR1 was detected in samples from Asia and North America. The brine shrimp cyst samples tested covered seven different species, with BSR1-positive samples found in <em>A. franciscana</em>, <em>A. urmiana</em>, <em>A. sinica</em>, and parthenogenetic <em>Artemia</em> lineage. The diagnostic sensitivity (DSe) and diagnostic specificity (DSp) values of the newly developed RT-qPCR method, when compared to the high-throughput sequencing, were determined to be 100 % and 88 %. This newly developed RT-qPCR method can serve as a crucial tool for detecting BSR1, facilitating early warning and swift response to viruses carried by brine shrimp, thereby effectively mitigating the risk of pathogen transmission to cultured animals.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742444"},"PeriodicalIF":3.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and metabolomic analysis reveals molecular mechanisms of infectious myonecrosis virus (IMNV) pathogenesis in Litopenaeus vannamei muscle tissue","authors":"Yan Rong ,&nbsp;Yi Qiao ,&nbsp;Xiaohui Cao ,&nbsp;Ge Jiang ,&nbsp;Jie Cheng ,&nbsp;Huiwen Jiang ,&nbsp;Hui Shen","doi":"10.1016/j.aquaculture.2025.742443","DOIUrl":"10.1016/j.aquaculture.2025.742443","url":null,"abstract":"<div><div>The Infectious myonecrosis virus (IMNV) poses a significant threat to <em>Litopenaeus vannamei</em>, and there is limited research on the transcriptomic and metabolomic changes in shrimp muscle tissue post-infection. This study aims to integrate transcriptomic and metabolomic data to elucidate the gene expression and metabolic alterations in shrimp muscle tissue induced by IMNV. We present a molecular model of IMNV invasion and the mechanisms leading to muscle cell necrosis. Sequencing analyses at 30, 60, and 90 days post-infection identified 695, 2411, and 401 differentially expressed genes (DEGs) and 118, 131, and 68 significantly different metabolites (SDMs), respectively. In the early infection stage (IE, 30 days), we observed alterations in gene expression related to glucose and lipid metabolism, along with upregulation of antioxidant-related genes. The intermediate stage (IM, 60 days) exhibited significant reprogramming of metabolic pathways and signal transduction. In the persistent infection stage (IP, 90 days), we noted significant changes in energy metabolism and cell repair-related gene expression, with upregulation of genes involved in glycolysis and fatty acid biosynthesis to maintain energy supply.</div><div>Our proposed model for IMNV invasion encompasses viral receptor recognition, endosomal escape, replication, and maintenance of the host cell environment. IMNV binds to host cells via the laminin receptor (Lamr), with the SMPD1 gene facilitating endosomal escape through ceramide production. The PI3K-Akt-mTOR pathway provides energy for viral replication, while the JAK-STAT pathway may be hijacked by IMNV. The virus reprograms metabolism by regulating pathways and metabolites such as <span>d</span>-glucose-6-phosphate, D-aspartic acid, and L-glutamic acid, while enhancing autophagy and regulating sphingolipid metabolism and antioxidant mechanisms. During viral particle assembly and release, SMPD1 promotes necroptosis and ceramide production. Additionally, IMNV modulates cytoskeletal remodeling and adhesion by regulating the Act57B, Act5C, and Act88F genes. IMNV leads to host muscle cell necrosis by suppressing the immune response, inducing cytoskeletal remodeling, oxidative stress, and activating cell death pathways such as apoptosis and ferroptosis. These processes disrupt the structure and function of muscle cells, leading to extensive necrosis. This study provides insights into the pathogenic mechanisms of IMNV and establishes a foundation for the development of anti-IMNV strategies.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"604 ","pages":"Article 742443"},"PeriodicalIF":3.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}