Aquaculture最新文献_第2页

Improved swimming performance from exercise training is driven by optimized muscle energy metabolism and antioxidant capacity in juvenile large yellow croaker Larimichthys crocea 运动训练对大黄鱼幼鱼游泳性能的改善是由优化的肌肉能量代谢和抗氧化能力驱动的

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-05 DOI: 10.1016/j.aquaculture.2026.743621

Sijian Deng , Yuhe Yang , Tingting Lin , Xin Liu , Yazhou Jiang , Zhengli Fang , Siping Li

Swimming performance is a critical locomotor trait that directly influences fish survival and reproductive success, playing an increasingly recognized role in the conservation and recovery of wild fish populations. Although exercise training can enhance swimming performance through both muscular aerobic and anaerobic metabolic phenotypes, the specific effects and underlying mechanisms remain poorly understood in the large yellow croaker (Larimichthys crocea). Herein, we subjected 5 cm juvenile large yellow croaker to exercise training at intensities of 0, 1, 2, and 4 BL/s for 6 h per day over 30 d, and evaluated both critical swimming speed (U_crit) and constant acceleration speed (U_cat). Behavioral results showed that training at 4 BL/s significantly improved U_crit and U_cat, with increases of 34.65 % and 24.66 %, respectively. Physiological analysis indicated that all exercise groups exhibited a significant increase in lactate dehydrogenase (LDH) activity and a significant decrease in 5-hydroxytryptamine (5-HT) concentration. In contrast, superoxide dismutase (SOD) activity significantly declined only in 2 BL/s, and cytochrome c oxidase (COX) concentration remained unchanged across all groups. Non-targeted metabolomics identified 143 significantly altered metabolites, primarily enriched in pathways including “glycerolipid metabolism”, “glycine, serine and threonine metabolism”, “choline metabolism in cancer”, “lysine degradation”, and “nucleotide metabolism”. Integrated transcriptomic and metabolomic analysis revealed that exercise training enhanced muscular metabolic phenotype by modulating key metabolites such as palmitoylcarnitine, glycerol-3-phosphate, allysine, guanosine, hypoxanthine, and glycerophosphocholine. These changes facilitated more efficient substrate utilization, optimized energy allocation, and improved antioxidant capacity, collectively contributing to the remodeling of muscular and behavioral performance. This study demonstrates that exercise training can effectively promote behavioral fitness in stock-enhanced fish species and provides insights into the metabolic basis underlying these improvements.

游泳是一种重要的运动特征，直接影响鱼类的生存和繁殖成功，在野生鱼类种群的保护和恢复中发挥着越来越重要的作用。尽管运动训练可以通过肌肉有氧和无氧代谢表型来提高游泳成绩，但在大黄鱼（Larimichthys crocea）中，具体效果和潜在机制仍然知之甚少。在此，我们对5厘米的大黄鱼幼鱼进行了0、1、2和4 BL/s强度的运动训练，每天6小时，持续30天，并评估了临界游泳速度（Ucrit）和恒定加速速度（Ucat）。行为学结果显示，4 BL/s训练显著提高了Ucrit和Ucat，分别提高了34.65%和24.66%。生理分析表明，所有运动组均表现出乳酸脱氢酶（LDH）活性显著增加，5-羟色胺（5-HT）浓度显著降低。相反，超氧化物歧化酶（SOD）活性仅在2 BL/s时显著下降，细胞色素c氧化酶（COX）浓度在各组间保持不变。非靶向代谢组学鉴定出143种显著改变的代谢物，主要富集于“甘油脂代谢”、“甘氨酸、丝氨酸和苏氨酸代谢”、“癌症中胆碱代谢”、“赖氨酸降解”和“核苷酸代谢”等途径。综合转录组学和代谢组学分析显示，运动训练通过调节关键代谢物，如棕榈酰基肉碱、甘油-3-磷酸、丙氨酸、鸟苷、次黄嘌呤和甘油磷胆碱，增强了肌肉代谢表型。这些变化促进了更有效的底物利用，优化了能量分配，提高了抗氧化能力，共同促进了肌肉和行为表现的重塑。这项研究表明，运动训练可以有效地促进种群增强型鱼类的行为健康，并为这些改善背后的代谢基础提供了见解。

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引用次数: 0

Adipose tissue-secreted Igf2 during promoting adipocyte hyperplasia improves muscle texture caused by high-fat diet through inhibiting myostatin in grass carp (Ctenopharyngodon idellus) 促进脂肪细胞增生过程中脂肪组织分泌的Igf2通过抑制肌肉生长抑制素改善草鱼高脂饲料引起的肌肉质地

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-04 DOI: 10.1016/j.aquaculture.2026.743618

Zhiqi Tian , Handong Li , Mingkui Wei , Hong Ji , Gen He , Jian Sun

With the rapid development of the aquaculture industry, it encounters several challenges, notably the increased costs of fishmeal protein sources. Although dietary lipid supplementation can decrease protein demand, high-fat diet (HFD) often compromises the aquatic products' muscle texture. Adipose tissue is an organ for storing energy. Recent study indicates that altering its expansion pattern by promoting adipocyte hyperplasia increased the tolerance of fish to HFD; however, in this context, whether the muscle texture could be improved remains elusive. A total of 270 subadult grass carp (Ctenopharyngodon idellus) (357.04 ± 1.32 g) were randomly allocated into 15 net cages. The experimental group received a HFD supplemented with the peroxisome proliferators-activated receptors γ (pparγ) agonist rosiglitazone to augment adipocyte hyperplasia. Promoting adipocyte hyperplasia significantly increased growth performance and enhanced muscle texture of grass carp fed with HFD, in the condition of raw and cooked meat, including spring, shear force, gumminess, chewiness, cohesiveness, and hardness (P < 0.05). Mechanistic investigations showed that promoting adipocyte hyperplasia increased the secretion of adipokine insulin-like growth factor-2 (Igf2). Subsequently, myogenic differentiation was promoted via myostatin expression inhibition in the muscle (P < 0.05), thereby improving the muscle texture of grass carp fed with HFD. Our data proved the existence of adipose tissue-muscle crosstalk driven by Igf2 and myostatin during promoting adipocyte hyperplasia. Thus, this research suggests targeting adipocyte expansion patterns via nutritional interventions, especially by incorporating pparγ-activating compounds, could be utilized to develop functional feed additives to counteract HFD's negative impacts on muscle texture.

随着水产养殖业的快速发展，它遇到了一些挑战，特别是鱼粉蛋白质来源的成本增加。虽然膳食脂质补充可以减少蛋白质需求，但高脂肪饮食（HFD）往往会损害水产品的肌肉质地。脂肪组织是储存能量的器官。最近的研究表明，通过促进脂肪细胞增生来改变其扩张模式可以增加鱼类对HFD的耐受性；然而，在这种情况下，肌肉纹理是否可以改善仍然是难以捉摸的。试验选取草鱼（Ctenopharyngodon idellus） 270尾（357.04±1.32 g），随机放入15个网箱中。实验组接受添加过氧化物酶体增殖物激活受体γ (pparγ)激动剂罗格列酮的HFD，以增加脂肪细胞增生。促进脂肪细胞增生显著提高了草鱼生肉和熟肉条件下的生长性能和肌肉质地，包括弹性、剪切力、胶性、咀嚼性、黏结性和硬度（P < 0.05）。机制研究表明，促进脂肪细胞增生可增加脂肪因子胰岛素样生长因子-2 （Igf2）的分泌。随后，通过抑制肌肉中肌肉生长抑制素的表达，促进了草鱼的成肌分化（P < 0.05），从而改善了饲料中草鱼的肌肉质地。我们的数据证明，在促进脂肪细胞增生过程中，存在由Igf2和肌肉生长抑制素驱动的脂肪组织-肌肉串扰。因此，本研究表明，通过营养干预，特别是通过添加γ γ γ活化化合物来靶向脂肪细胞的扩张模式，可以用来开发功能性饲料添加剂，以抵消HFD对肌肉质地的负面影响。

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引用次数: 0

Total suspended solids induced gill oxidative damage and inflammation in tilapia: Reversibility upon transition to clear-water systems revealed by integrated transcriptomics and histopathology 总悬浮固体诱导的罗非鱼鳃氧化损伤和炎症：综合转录组学和组织病理学揭示的向清水系统过渡的可逆性

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-02 DOI: 10.1016/j.aquaculture.2025.743617

Yanshuo Guo , Jincheng Lin , Wenchang Liu , Changqing Wang , Guozhi Luo , Hongxin Tan

Total suspended solids (TSS) commonly accumulate in biofloc technology (BFT) systems and can compromise fish health in intensive culture. In this study, a staged transition from BFT (high TSS) to a recirculating clear-water (CW) system was evaluated in tilapia (genetically improved Oreochromis niloticus), with continuous BFT serving as the control. During the CW stage, TSS was maintained at <70 mg/L, whereas single BFT exceeded 1500 mg/L. Gill histology showed progressive recovery after transition, accompanied by increased mucous cell counts. Enzyme assays indicated alleviated oxidative damage and restoration of osmoregulatory and energy-metabolism enzyme activities. Integrative transcriptomic analyses revealed that high TSS elicited severe gill injury characterized by oxidative stress, chronic inflammation, and apoptosis, whereas transition to CW reversed these responses by upregulating antioxidant defenses, suppressing inflammatory pathways, and promoting tissue repair. Improved environmental conditions also enhanced growth performance, yielding superior culture outcomes. Collectively, the findings demonstrate that a BFT to CW transition effectively mitigates TSS-induced physiological stress and improves fish health, providing a practical strategy for sustainable intensive aquaculture.

总悬浮固体（TSS）通常在生物絮团技术（BFT）系统中积累，并可能在集约化养殖中危害鱼类健康。在本研究中，以罗非鱼（转基因尼罗鱼）为对照，评价了从BFT（高TSS）到循环水（CW）系统的阶段性过渡。连续期TSS维持在70 mg/L，单次BFT超过1500 mg/L。鳃组织学显示转化后逐渐恢复，并伴有黏液细胞计数增加。酶分析表明，氧化损伤减轻，渗透调节和能量代谢酶活性恢复。综合转录组学分析显示，高TSS引发了以氧化应激、慢性炎症和细胞凋亡为特征的严重鳃损伤，而向CW的转变通过上调抗氧化防御、抑制炎症通路和促进组织修复来逆转这些反应。改善的环境条件也提高了生长性能，产生了优异的培养效果。综上所述，BFT向CW过渡可有效缓解tss引起的生理应激，改善鱼类健康，为可持续集约化养殖提供实用策略。

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引用次数: 0

Size limits for the use of Ballan wrasse (Labrus bergylta) as cleaner fish in salmon aquaculture cages 使用巴兰濑鱼（Labrus bergylta）作为鲑鱼养殖网箱清洁鱼的尺寸限制

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-02 DOI: 10.1016/j.aquaculture.2025.743613

Terje Jørgensen , Manu Sistiaga , Bent Herrmann

Ballan wrasse (Labrus bergylta) is an important cleaner fish species used by the salmon farming industry for sea louse (Lepeophtheirus salmonis) control and demand for it is high. However, supply of wild-caught fish is limited and most ballan wrasse used by the industry are farmed individuals. Ballan wrasse can escape from aquaculture cages if netting mesh size is too large for retention. Further, mesh shape and state can also affect the size of ballan wrasse that can escape through the cage netting. Escaping fish may result in genetic contamination of local fish populations through hybridization and spread of diseases. Based on morphology measurements combined with fall through tests, the escape risk of ballan wrasse was predicted using computer simulation. The simulations considered fish morphology and tissue compressibility, and square meshes between 10 and 100 mm in stiff, semi-slack and slack states. The predictions showed that the escape risk increases with mesh size and mesh openness, peaking at an openness of ca. 90 %, and was highest for slack meshes. Size selectivity results from crowding experiments in the laboratory complied well with the simulation results and demonstrated the importance of considering mesh states other than stiff meshes when evaluating the potential escape of ballan wrasse through meshes in aquaculture cages. Finally, the morphology of wild and farmed ballan wrasse was compared to conclude that size selectivity predictions for farmed ballan wrasse cannot be used to predict the escape risk for wild fish because the latter exhibits a smaller cross section.

巴兰濑鱼（Labrus bergylta）是鲑鱼养殖业用于控制海虱（Lepeophtheirus salmonis）的重要清洁鱼类，需求量很大。然而，野生捕捞的鱼类供应有限，该行业使用的大多数巴兰濑鱼都是养殖的个体。如果网目尺寸太大而无法保留，巴兰濑鱼可以从水产养殖笼中逃脱。此外，网眼的形状和状态也会影响通过网眼逃跑的濑鱼的大小。逃逸的鱼类可能通过杂交和疾病传播导致当地鱼类种群的遗传污染。在形态测量与坠井试验相结合的基础上，利用计算机模拟预测了濑鱼的逃生风险。模拟考虑了鱼类形态和组织可压缩性，以及在僵硬、半松弛和松弛状态下10至100毫米之间的方形网格。预测表明，逃逸风险随着网格大小和网格开放度的增加而增加，在开放度约为90%时达到峰值，松弛网格的逃逸风险最高。实验室拥挤实验的尺寸选择性结果与模拟结果吻合较好，表明在评估养殖网箱网眼逃逸可能性时，考虑网眼状态而非刚性网眼的重要性。最后，对野生和养殖白濑鱼的形态进行了比较，得出结论：养殖白濑鱼的大小选择性预测不能用于预测野生鱼类的逃跑风险，因为后者具有较小的横截面。

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引用次数: 0

Effect of transport temperature and duration on physicochemical and metabolic properties of Pelteobagrus fulvidraco 运输温度和时间对黄颡鱼理化代谢特性的影响

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-01 DOI: 10.1016/j.aquaculture.2025.743612

Chan Bai , Zhaoding Wang , Jinxiu Wang , Li Zhou , Juguang Wang , Yi Chai , Guangquan Xiong , Lan Wang , Tao Liao

Transport stress during live fish transit is a major challenge in aquaculture. This study employed an integrated approach, combining serum biochemistry, liver histopathology, and muscle metabolomics, to assess the physiological and metabolic responses of Pelteobagrus fulvidraco to simulated transport at different temperatures (10, 14, 18, and 22 °C) and durations (0–24 h). Key findings revealed that transport stress significantly (p < 0.05) elevated serum levels of cortisol, glucose, and malondialdehyde, and induced marked liver damage. Metabolomic analysis of muscle tissue from sampled fish (n = 4 per group) demonstrated a time-dependent shift in the global metabolic response, transitioning from bile acid biosynthesis to purine metabolism. Furthermore, temperature significantly influenced the specific metabolic profile, with energy-related pathways dominating at 10 °C and 18 °C, while a shift towards nucleotide degradation products was notable at 14 °C. An integrated assessment identified 18 °C as the optimal transport temperature, causing the least physiological and metabolic disruption. These findings provide a scientific framework for optimizing transport conditions to mitigate stress and improve welfare in aquaculture, with the identified biomarkers holding promise for broader application in farmed fish species.

活鱼运输过程中的运输应激是水产养殖面临的主要挑战。本研究采用综合方法，结合血清生化、肝脏组织病理学和肌肉代谢组学，评估黄颡鱼（Pelteobagrus fulvidraco）在不同温度（10、14、18和22°C）和时间（0-24 h）下对模拟运输的生理和代谢反应。主要研究结果显示，运输应激显著（p < 0.05）升高血清皮质醇、葡萄糖和丙二醛水平，并诱导明显的肝损伤。对鱼样本肌肉组织的代谢组学分析（每组n = 4）表明，整体代谢反应发生了时间依赖性转变，从胆汁酸生物合成过渡到嘌呤代谢。此外，温度显著影响了特定的代谢谱，在10°C和18°C时，能量相关的途径占主导地位，而在14°C时，向核苷酸降解产物的转变是显著的。综合评估确定18°C为最佳运输温度，造成的生理和代谢干扰最小。这些发现为优化运输条件以减轻压力和提高水产养殖福利提供了科学框架，所鉴定的生物标志物有望在养殖鱼类中得到更广泛的应用。

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引用次数: 0

Gaping restriction as a mechanism to mitigate live transport and re-immersion physiological stress in mussels 间隙限制作为减轻贻贝活体运输和再浸泡生理应激的机制

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-31 DOI: 10.1016/j.aquaculture.2025.743614

Leonardo N. Zamora , David J. Burritt , Norman L.C. Ragg , Paula Casanovas , Natalí J. Delorme

Many shellfish species are intentionally taken out of water at different times throughout their farming cycle; especially those sold to live markets. For example, mussels can spend days out of the water after harvest during transport. In this study, we evaluated the potential to enhance recovery in seawater after aerial exposure in the green-lipped mussel, Perna canaliculus. Survival and oxidative stress kinetics were assessed in mussels with no gaping restriction (NGR) versus mussels in which gaping was moderately (MGR) or fully restricted (FGR) during different emersion periods (3, 5 or 7 days (d) after harvest) and their ability to recover in seawater. Results suggested that gaping restriction had a positive effect on re-immersion recovery survival after 3 d of emersion (43 % higher in FGR and 78 % higher in MGR mussels); however, the benefits were time-dependent, as survival decreased after 5 and 7 d of emersion, regardless of gaping restriction treatment. This was reflected in the changes observed in the oxidative stress biomarkers of mussels emersed for 7 d before re-immersion. These mussels appeared unable to compensate for the accumulated damage during emersion and subsequent reoxygenation stress. Damage was apparent as accumulation of protein carbonyls, lipid hydroperoxide and 8-hydroxydeoxyguanosine within one hour of re-immersion in seawater, with all mussels dying within the following ten days. In mussels that recovered after 3 or 5 d of emersion, levels of oxidative damage decreased within 8 h of re-immersion for the NGR and FGR mussels, while the MGR mussels showed no significant reoxygenation damage after 3 d of emersion and recovered faster (i.e., within 4 h) after 5 d of emersion. The antioxidant enzymes showed similar trends in all gaping restriction treatments, and only catalase and glutathione peroxidase activities decreased within ten days after re-immersion. Understanding the changes over time of the mussels' antioxidant capacity and oxidative damage helps explain emersion thresholds for recovery survival. This study presents a promising tool (i.e., gaping restriction) for consideration to enhance the mussels' ability to cope with live transport, and the subsequent reoxygenation stress when mussels are placed back in seawater in live shellfish markets.

许多贝类在整个养殖周期的不同时间被有意地从水中取出；尤其是那些卖给活体市场的。例如，贻贝在收获后的运输过程中可能要离开水好几天。在这项研究中，我们评估了绿唇贻贝（Perna canaliculus）在空气暴露后在海水中提高恢复的潜力。在不同的重现期（收获后3、5或7天），研究人员评估了无开口限制（NGR）贻贝与适度开口限制（MGR）或完全开口限制（FGR）贻贝的生存和氧化应激动力学，以及它们在海水中的恢复能力。结果表明，开口限制对重新浸泡3 d后的恢复存活率有积极影响（FGR贻贝提高43%，MGR贻贝提高78%）；然而，这种益处是有时间依赖性的，无论是否进行间隙限制治疗，存活时间都会在5天和7天后下降。这反映在重新浸泡前7天贻贝氧化应激生物标志物的变化中。这些贻贝似乎无法补偿在恢复和随后的再氧化应激期间累积的损伤。贻贝在海水中再次浸泡1小时内，蛋白质羰基、脂质过氧化氢和8-羟基脱氧鸟苷的积累对贻贝造成了明显的损害，10天内全部死亡。在浸泡3 d或5 d后恢复的贻贝中，NGR和FGR贻贝的氧化损伤水平在浸泡8 h内下降，而MGR贻贝在浸泡3 d后没有明显的再氧化损伤，在浸泡5 d后恢复得更快（即在4 h内）。抗氧化酶的变化趋势在所有限制处理中都相似，只有过氧化氢酶和谷胱甘肽过氧化物酶活性在重新浸泡后10 d内下降。了解贻贝抗氧化能力和氧化损伤随时间的变化有助于解释恢复生存的再现阈值。本研究提出了一种很有前途的工具（即缺口限制），可以考虑提高贻贝在活贝类市场中应对活运输的能力，以及贻贝被放回海水后的再氧化应激。

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引用次数: 0

Multi-omics insights into the adaptation of sea urchins (Strongylocentrotus intermedius) to ocean oxygen fluctuations 多组学研究海胆（Strongylocentrotus intermedius）对海洋氧气波动的适应

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-31 DOI: 10.1016/j.aquaculture.2025.743610

Yi Chen , Yuntian Zhang , Guanying Lv , Jiadong Guo , Rongwei Zhang , Xiaoran Zhao , Yuzhe Han , Tongjun Ren

Oxygen fluctuations strongly affect marine invertebrates, such as the sea urchins (Strongylocentrotus intermedius). To examine its physiological and molecular responses to dissolved oxygen (DO) fluctuations, individuals were exposed for 50 days to hypoxia (LO: 4.0 ± 0.5 mg/L), normoxia (NO: 7.5 ± 0.5 mg/L), or hyperoxia (HO: 23.5 ± 0.5 mg/L). We evaluated growth performance, coelomic fluid biochemistry, gut microbiota, and gonadal transcriptomics. S. intermedius in the HO group showed significantly higher weight gain rate (WGR), specific growth rate (SGR), and gonadosomatic index (GI) than those in the LO and NO groups. In coelomic fluid, oxidative stress was elevated in both LO and HO groups compared with NO, and was highest under LO; adenosine triphosphate (ATP) and glucose (GLU) levels increased in hyperoxia, alongside a reduction in total amino acids (T-AA); oxygen pressure [P(O₂)] rose with increasing DO, whereas H⁺ and HCO₃⁻ concentrations shifted among treatments. Gut microbiota composition also changed with DO levels: Proteobacteria abundance increased under hyperoxia, while overall microbial diversity remained stable under normoxia, as indicated by Shannon and Simpson indices. Integrated 16S rRNA and RNA-seq analyses revealed parallel metabolic adaptations in gut microbiota and host under hyperoxia, particularly in lipid, amino acid, and carbohydrate metabolism, these highlighting host-microbe cooperation in oxygen fluctuations. Gonadal transcriptome analysis (HO vs. NO) identified 1781 differentially expressed genes (DEGs), validated by RT-qPCR, enriched in pathways related to cholesterol metabolism, fat digestion and absorption, pancreatic-like secretion, and protein digestion. These results indicate that S. intermedius uses physiological, microbial, and molecular strategies to cope with DO fluctuations, providing new insights into echinoderm resilience in changing marine environments.

氧气波动强烈影响海洋无脊椎动物，如海胆（strongylocentrrotus intermedius）。为了研究其对溶解氧（DO）波动的生理和分子反应，将个体暴露在低氧（LO: 4.0±0.5 mg/L）、常氧（NO: 7.5±0.5 mg/L）或高氧（HO: 23.5±0.5 mg/L）环境中50天。我们评估了生长性能、体腔流体生化、肠道微生物群和性腺转录组学。HO组中鳉鱼的增重率（WGR）、特定生长率（SGR）和促性腺指数（GI）显著高于LO和NO组。在体腔液中，与NO相比，LO组和HO组氧化应激均升高，且LO组最高；三磷酸腺苷（ATP）和葡萄糖（GLU）水平在高氧状态下升高，同时总氨基酸（T-AA）减少；氧压[P(O2)]随DO的增加而升高，而H+和HCO3−浓度在不同处理之间发生变化。肠道菌群组成也随着DO水平的变化而变化：Shannon和Simpson指数表明，高氧条件下变形菌群丰度增加，而常氧条件下总体微生物多样性保持稳定。综合16S rRNA和RNA-seq分析揭示了高氧条件下肠道微生物群和宿主的平行代谢适应，特别是在脂质、氨基酸和碳水化合物代谢方面，这些突出了宿主-微生物在氧波动中的合作。性腺转录组分析（HO vs. NO）鉴定了1781个差异表达基因（DEGs），并通过RT-qPCR验证，这些基因在胆固醇代谢、脂肪消化和吸收、胰腺样分泌和蛋白质消化等相关途径中富集。这些结果表明，中间棘球蚴利用生理、微生物和分子策略来应对DO波动，为研究棘皮动物在变化的海洋环境中的恢复力提供了新的见解。

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引用次数: 0

Establishment of a spinal cord-derived cell line from Micropterus salmoides reveals differential host-pathogen interactions and innate immune evasion strategies 小翼鱼脊髓源性细胞系的建立揭示了不同宿主-病原体相互作用和先天免疫逃避策略

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-31 DOI: 10.1016/j.aquaculture.2025.743611

Xin Chu , Lingyun Lin , Jinpeng Gong , Mingyue Gao , Jinyu Shen , Yan Zhang , Xiaoyi Pan

The establishment of fish cell lines is important for advancing our understanding of fish diseases and their underlying molecular mechanisms. Here, we report the development and characterization of a continuous spinal cord-derived cell line from Micropterus salmoides, which successfully maintained for over 120 passages. Species origin was confirmed by sequencing the mitochondrial 16S rRNA, cytochrome c oxidase subunit 1 (CO1), and cytochrome b (Cytb) genes. Karyotype analysis revealed that the cells possessed a diploid chromosome number of 2n = 46. Following transfection with the pEGFP-N1 plasmid, the cells exhibited distinct green fluorescence and demonstrated the ability to tolerate high-temperature culture conditions at 37 °C. Crucially, the MSSC cell line exhibited high susceptibility to largemouth bass rhabdovirus (MSRV), largemouth bass ranavirus (LMBV), and infectious spleen and kidney necrosis virus (ISKNV), with viral titers reaching 10^7.10, 10^8.52, and 10^6.17 TCID₅₀/mL, respectively. This was demonstrated by the occurrence of severe cytopathic effects (CPE) and abundant virus particles observed within the cytoplasm. Additionally, infection of MSSC cells with the three viruses induced nuclear morphological changes and significant reorganization of the actin cytoskeleton, characterized by nuclear condensation and fragmentation, along with a marked reduction in actin stress fibers. Notably, ISKNV infection resulted in the formation of filamentous pseudopodia. In all virus-infected MSSC cells, immune-related genes including TNF-α, IL-1β, STAT1, and MX were upregulated, cGAS and STING were significantly upregulated only in response to LMBV infection. In contrast, the DNA virus ISKNV failed to induce this pathway, suggesting a sophisticated viral immune evasion strategy. The newly established MSSC cell line provides a unique and powerful platform for virus isolation and amplification, mechanistic studies of host–pathogen interactions and innate immunity, and temperature-resolved experimentation (including sustained culture at 37 °C) in an economically important aquaculture species.

鱼类细胞系的建立对于提高我们对鱼类疾病及其潜在分子机制的认识具有重要意义。在这里，我们报道了一个连续的脊髓来源的小翼鱼细胞系的发展和特征，它成功地维持了超过120传代。通过对线粒体16S rRNA、细胞色素c氧化酶亚基1 （CO1）和细胞色素b （Cytb）基因的测序，证实了物种起源。核型分析表明，细胞具有二倍体染色体数2n = 46。转染pEGFP-N1质粒后，细胞显示出明显的绿色荧光，并表现出耐受37℃高温培养条件的能力。最重要的是，MSSC细胞系对大嘴黑鲈横纹蛇病毒（MSRV）、大嘴黑鲈ranv （LMBV）和感染性脾肾坏死病毒（ISKNV）表现出高易感，病毒滴度分别达到107.10、108.52和106.17 TCID50/mL。严重的细胞病变效应（CPE）和细胞质内观察到的大量病毒颗粒证明了这一点。此外，三种病毒感染MSSC细胞诱导细胞核形态改变和肌动蛋白细胞骨架的显著重组，以核凝聚和断裂为特征，同时肌动蛋白应激纤维明显减少。值得注意的是，ISKNV感染导致丝状假足的形成。在所有病毒感染的MSSC细胞中，免疫相关基因包括TNF-α、IL-1β、STAT1和MX上调，cGAS和STING仅在LMBV感染时显著上调。相比之下，DNA病毒ISKNV未能诱导这一途径，这表明一种复杂的病毒免疫逃避策略。新建立的MSSC细胞系为病毒分离和扩增、宿主-病原体相互作用和先天免疫的机制研究以及在经济上重要的水产养殖物种中进行温度解决实验（包括37°C持续培养）提供了独特而强大的平台。

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引用次数: 0

Microbiota of larval red drum (Sciaenops ocellatus): Influence of parental diet 红颡鱼幼鱼的微生物群：亲本饮食的影响

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-30 DOI: 10.1016/j.aquaculture.2025.743609

Andrea M. Tarnecki , Cynthia K. Faulk , Lee A. Fuiman

Variability in survival of larval stages represents a significant hurdle in finfish aquaculture. Research suggests that parental diet may influence the microbiota of resulting offspring, yet this hypothesis has remained unexplored. To understand the relationship between broodstock diet and larval bacterial communities, this study investigated the microbiota composition of 7- and 10-day post-hatch (dph) larvae of red drum (Sciaenops ocellatus) collected from 11 spawning events. Parental broodstock populations were fed a mixture of shrimp, squid, and sardines (Full diet) or shrimp only (Shrimp diet). Fatty acid composition of eggs was influenced by parental diet, whereas that of larvae was driven by larval feeds (rotifers, Artemia). Importantly, microbiota structure was different between larvae originating from parents fed the Shrimp versus the Full diets. Correlations between fatty acid profiles and the larval microbiota indicate that host (larval) fatty acids impact and respond to the microbiota. These results suggest a mechanism by which broodstock diet determines yolk composition, which alters adherence sites and selects for unique microbiota. These communities differently metabolize fatty acids and alter their availability for absorption by the host. As microbiota exert strong influence on larval growth and survival, exploring links between larva-associated bacteria and broodstock is important for optimizing marine finfish larviculture.

幼虫期存活率的变化是鳍鱼养殖的一个重大障碍。研究表明，父母的饮食可能会影响后代的微生物群，但这一假设尚未得到证实。为了解饲料与幼鱼细菌群落的关系，本研究对11个产卵事件中采集的红颡鱼（Sciaenops ocellatus）孵化后7天和10天幼虫的微生物群组成进行了研究。父母养殖种群的混合喂养虾、鱿鱼、沙丁鱼(完整的饮食)或只虾(虾饮食)。卵的脂肪酸组成受亲代饮食的影响，而幼虫的脂肪酸组成受幼虫饲料（轮虫、蒿）的影响。重要的是，父母喂养对虾和完全饮食的幼虫的微生物群结构不同。脂肪酸谱与幼虫微生物群之间的相关性表明，宿主（幼虫）脂肪酸影响并响应微生物群。这些结果提示了一种机制，通过饲料决定卵黄组成，改变粘附位点和选择独特的微生物群。这些群落以不同的方式代谢脂肪酸，并改变它们被宿主吸收的有效性。由于微生物群对鱼类幼鱼的生长和存活具有重要影响，因此探索与幼鱼相关的细菌与亲鱼之间的联系对于优化海洋鱼类幼鱼养殖具有重要意义。

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引用次数: 0

Dynamics of intestinal organization and microbiota during early development of Procypris mera 原螺旋体发育早期肠道组织和微生物群的动态变化

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2025-12-30 DOI: 10.1016/j.aquaculture.2025.743599

Lilong Chen , Junquan Ren , Ye Tian , Xiaoqi Tang , Jingting Yao , Weijun Wu , Zhe Li , Yong Lin , Hua Ye , Yusen Li , Shengqi Su

The development of the fish digestive system, especially during early growth stages, is critical for advancing fish feeding techniques. This study investigated the intestinal structure and microbiota of early developmental stages of Procypris mera using conventional paraffin sectioning and 16S rDNA sequencing technologies. We also analyzed the correlations between intestinal microbiota and bait/water flora. Results showed that P. mera intestinal structure development progressed with fish growth, marked by the emergence of intestinal glands at 50 DAH (Days After Hatching), indicating near completion of intestinal maturation. The intestinal flora diversity fluctuated during early development, with the microbiota primarily composed of Proteobacteria and Firmicutes. Before first feeding, intestinal microbiota likely originated from maternal inheritance and water environment. After first feeding, the similarity between intestinal and water microbiota was higher than that between intestinal and bait. At the phylum level, shared dominant bacterial flora were observed in both the intestinal-water and intestinal-bait comparisons, whereas no dominant bacterial flora was shared at the genus level. This study provides valuable theoretical insights into gut microbiota formation during early fish development, contributing to healthy fish culture.

鱼类消化系统的发育，特别是在早期生长阶段，对提高鱼类饲养技术至关重要。本研究采用常规石蜡切片技术和16S rDNA测序技术对mera Procypris发育早期的肠道结构和微生物群进行了研究。我们还分析了肠道微生物群与饵料/水中菌群的相关性。结果表明，mera P. mera肠道结构的发育随着鱼的生长而进展，在孵化后50天出现肠腺，表明肠道成熟接近完成。肠道菌群多样性在发育早期波动，微生物群主要由变形菌门和厚壁菌门组成。在首次喂养之前，肠道微生物群可能来源于母体遗传和水环境。首次投食后，肠道微生物群与水中微生物群的相似性高于肠道与饵料微生物群的相似性。在门水平上，在肠道-水和肠道-饵的比较中观察到共同的优势菌群，而在属水平上没有共同的优势菌群。该研究为鱼类早期发育过程中肠道菌群的形成提供了有价值的理论见解，有助于健康的鱼类养殖。

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引用次数: 0