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Accumulation patterns and chronic toxic effects of triphenyl phosphine in Mytilus coruscus 三苯基膦在贻贝中的积累模式和慢性毒性效应
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-12 DOI: 10.1016/j.aquaculture.2024.741869
Ningbo Wang , Yinan Wang , Rongrong Zhang , Aili Sun , Yin Lu , Zeming Zhang , Xizhi Shi
Triphenylphosphine oxide (TPPO) as a new organophosphorus flame retardant is extensively used in the industrial and pharmaceutical fields due to its chemical stability and abundance. In marine environments, TPPO accumulates as a result of natural ecological processes. This study aimed to evaluate the accumulation process and toxicological effects of TPPO utilizing mussels (Mytilus coruscus), a species traditionally employed in marine monitoring and commonly cultivated in offshore aquaculture. GC–MS/MS was employed to quantify the accumulation of TPPO in the gill, gonad, muscle, and digestive gland tissues of mussels exposed to three sublethal concentrations (20.0, 100.0, and 500.0 μg/L) over durations of 3, 7, 14, and 28 days, as well as the examining of the chemical and molecular parameters. Results showed that the accumulation of TPPO (100.0 and 500.0 μg/L) increased throughout the 28 d of exposure, and the peak accumulation of 20.0 μg/L TPPO was detected on 14 d. Short-term TPPO exposure did not cause oxidative stress in mussels. Chronic exposure for 28 days resulted in significant oxidative damage and oxidative stress in mussels, as evidenced by elevated expression and activity of SOD, increased levels of glutathione and malondialdehyde, and upregulation of HSP70 and HSP90, metallothionein, and CYP4Y1, alongside the initiation of apoptotic cell death. TPPO exposure activated natural immune genes and disrupted lipid metabolism in mussels. This study demonstrated the persistent accumulation of TPPO in mussels, highlighting its ecotoxicological hazards and underscoring the necessity to predict the risks associated with widespread environmental exposure to TPPO.
三苯基膦氧化物(TPPO)作为一种新型有机磷阻燃剂,因其化学性质稳定、数量丰富而被广泛应用于工业和医药领域。在海洋环境中,TPPO 会随着自然生态过程而累积。本研究旨在利用贻贝(Mytilus coruscus)来评估 TPPO 的积累过程和毒理学效应。研究采用气相色谱-质谱/质谱(GC-MS/MS)分析了在 3、7、14 和 28 天三种亚致死浓度(20.0、100.0 和 500.0 μg/L)条件下,TPPO 在贻贝的鳃、生殖腺、肌肉和消化腺组织中的积累情况,并对化学和分子参数进行了检测。结果表明,在 28 天的接触过程中,TPPO(100.0 和 500.0 μg/L)的累积量不断增加,在 14 天时检测到 20.0 μg/L 的 TPPO 累积峰值。持续 28 天的慢性暴露会导致贻贝出现严重的氧化损伤和氧化应激,表现为 SOD 的表达和活性升高、谷胱甘肽和丙二醛的水平升高、HSP70 和 HSP90、金属硫蛋白和 CYP4Y1 的上调以及细胞凋亡的启动。暴露于 TPPO 会激活天然免疫基因并破坏贻贝的脂质代谢。这项研究证明了 TPPO 在贻贝中的持续积累,突出了其生态毒理学危害,并强调了预测与环境中广泛接触 TPPO 相关的风险的必要性。
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引用次数: 0
Propagation guide for Salicornia s. l. (Amaranthaceae, Magnoliophyta): A review focused on North American species Salicornia s. l. (Amaranthaceae, Magnoliophyta) 的繁殖指南:北美物种综述
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-12 DOI: 10.1016/j.aquaculture.2024.741868
Kennia Brum Doncato
Salicornia is a genus of species with high salt-tolerance (i.e. halophytes), and the most prominent edible plants for Saline Agriculture, Marine Aquaculture and both combined (IAAS – Integrated Agri-Aquaculture systems). They can be part of the coastal vegetation (e.g. salt marsh and mangroves), and as they are distributed in the interface between water and land, being a novel range of products to be included in Marine Aquaculture. In some places, their presence in nature can be in danger for diverse reasons. One of the bottlenecks for Salicornia production is the nursery stage, which was never fully explained as other sectors of Marine Aquaculture. In New England, there are three species of Salicornia (Salicornia ambigua, Salicornia bigelovii and Salicornia depressa) recorded more than a century ago. Since then, little progress has been made to establish protocols to propagate these species that are important for ecological and commercial purposes. Our aim was to synthesize all the nursery steps for controlled production of three Salicornia species, and it can also be adapted to other species.
盐生植物属(Salicornia)是一种具有高度耐盐性的物种(即盐生植物),也是盐碱地农业、海水养殖和两者结合(IAAS - 综合农业-水产养殖系统)的最主要食用植物。它们可以是沿海植被(如盐沼和红树林)的一部分,由于分布在水和陆地的交界处,因此是海洋水产养殖中的一种新产品。在一些地方,由于各种原因,它们在自然界中的存在可能面临危险。沙丁鱼生产的瓶颈之一是育苗阶段,这一点从未像海水养殖的其他部门那样得到充分解释。在新英格兰,一个多世纪前就记录了三种盐角藻(Salicornia ambigua、Salicornia bigelovii 和 Salicornia depressa)。从那时起,在制定繁殖这些对生态和商业目的都很重要的物种的规程方面进展甚微。我们的目标是总结出控制生产这三种盐生植物的所有育苗步骤,它也可适用于其他物种。
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引用次数: 0
Tannic acid reduced the growth performance, antioxidant, and immune functions of the Nile tilapia (Oreochromis niloticus) 单宁酸降低尼罗罗非鱼(Oreochromis niloticus)的生长性能、抗氧化性和免疫功能
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-10 DOI: 10.1016/j.aquaculture.2024.741872
Junhui Liu, Xingqian Zhang, Qifeng Lu, Huai Zhang, Li Lin, Qingqing Li
The Nile tilapia (Oreochromis niloticus) is highly regarded for its delicious taste and abundant nutritional value. With the growing demand for sustainable aquaculture, plant proteins have become crucial in fish feeds due to their cost-effectiveness and environmental benefits. Proteins such as soy protein, rapeseed meal, and faba bean protein have partially replaced traditional animal-derived proteins. However, these plant proteins often contain significant amounts of antinutritional factors, such as tannic acid (TA), and the effects of high doses on Nile tilapia remain not fully understood. The objective of this 60-day experiment was to evaluate how varying levels of TA affect the growth performance, intestinal structure, physiological indices, and immune functions of Nile tilapia. Five groups were designed for this experiment: TA0 (0 g/kg), TA1 (5 g/kg), TA2 (10 g/kg), TA3 (15 g/kg), and TA4 (20 g/kg). The results of the study showed that the body weight of Nile tilapia in the other TA groups was significantly lower compared to the TA0 groups (P < 0.05). The liver somatic index and viscerosomatic index of the TA0 group were significantly lower than those of the other TA groups (P < 0.05). Observations of intestine tissues indicated varying degrees of structural damage in the treatment groups. The muscle thickness of the TA0 was significantly greater than that of the TA4 group (P < 0.05). The villus width was significantly reduced in the TA3 and TA4 groups (P < 0.05), while the villus height in the TA0 group was significantly greater than that of the other groups (P < 0.05). Regarding physiological indicators, there were no notable variations in the levels of total antioxidant capacity and superoxide dismutase in the serum, liver, and intestine. However, catalase activity was significantly reduced in serum and liver (P < 0.05), with a decreasing trend in the levels of glucose-6-phosphate dehydrogenase observed in the serum, liver, and muscle tissues of the TA1, TA2, and TA3 groups. Also, the expression levels of Hsp70 and IL-1β were significantly upregulated in the liver, intestine, and muscle tissues of the treatment groups (P < 0.05). These results will help to clarify the effects of high concentrations of TA on Nile tilapia and provide valuable insights for selection of appropriate dosage of TA.
尼罗罗非鱼(Oreochromis niloticus)因其鲜美的味道和丰富的营养价值而备受推崇。随着可持续水产养殖需求的不断增长,植物蛋白因其成本效益和环境效益而成为鱼饲料的关键。大豆蛋白、菜籽粕和蚕豆蛋白等蛋白质已部分取代了传统的动物源性蛋白质。然而,这些植物蛋白通常含有大量的抗营养因子,如单宁酸(TA),而且高剂量对尼罗罗非鱼的影响仍未得到充分了解。这项为期 60 天的实验旨在评估不同水平的 TA 如何影响尼罗罗非鱼的生长性能、肠道结构、生理指标和免疫功能。本实验设计了五个组:TA0(0 克/千克)、TA1(5 克/千克)、TA2(10 克/千克)、TA3(15 克/千克)和 TA4(20 克/千克)。研究结果表明,与 TA0 组相比,其他 TA 组尼罗罗非鱼的体重明显较低(P < 0.05)。TA0组的肝脏体细胞指数和粘液指数明显低于其他TA组(P< 0.05)。对肠道组织的观察表明,各处理组均存在不同程度的结构性损伤。TA0 组的肌肉厚度明显大于 TA4 组(P < 0.05)。TA3和TA4组的绒毛宽度明显减少(P <0.05),而TA0组的绒毛高度明显高于其他组(P <0.05)。在生理指标方面,血清、肝脏和肠道中的总抗氧化能力和超氧化物歧化酶水平没有明显变化。不过,TA1、TA2 和 TA3 组血清和肝脏中过氧化氢酶活性明显降低(P < 0.05),血清、肝脏和肌肉组织中葡萄糖-6-磷酸脱氢酶水平呈下降趋势。此外,在治疗组的肝脏、肠道和肌肉组织中,Hsp70 和 IL-1β 的表达水平显著上调(P < 0.05)。这些结果将有助于澄清高浓度 TA 对尼罗罗非鱼的影响,并为选择合适的 TA 剂量提供有价值的见解。
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引用次数: 0
Antiparasitic effect of imidacloprid on the copepodite and adult stages of Caligus rogercresseyi 吡虫啉对桡足类和成虫的抗寄生效果
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-10 DOI: 10.1016/j.aquaculture.2024.741878
Margarita P. González-Gómez , Javier Campanini-Salinas , Paola Olmos-Iturrieta , Ananías Peralta , Marlen Espinoza , Jorge Mancilla-Schulz
In Chile, there are currently eight authorized pharmaceutical chemical compounds for the control of sea lice (Caligus rogercresseyi), namely emamectin benzoate, diflubenzuron, lufenuron, hexaflumuron, hydrogen peroxide, deltamethrin, cypermethrin and azamethiphos; however, being this range of tools considered scarce, moreover, loss of sensitivity to some of these compounds have been described in previous studies. Imidacloprid is a neonicotinoid synthetic insecticide that was authorized in Norway in 2021 and has revealed good efficacy in controlling Lepeophtheirus salmonis on salmon farms; however, it has not been authorized for use in Chile. Therefore, in this study, we investigated the efficacy of imidacloprid on the copepodite and adult stages of C. rogercresseyi, which, to our knowledge, has not been investigated in previous studies. We observed that imidacloprid immobilized the parasite at both stages; however, adults were able to recover after the exposure, with 50 % of the adults exposed to concentrations <40 ppm for 24 h recovering, suggesting that a concentration > 20 ppm is required for 100 % mortality of this parasite. In contrast, the copepodite stage was immobilized at 20 ppm, suggesting that copepodites are more sensitive to imidacloprid than the adults. Further studies are required to understand the biological responses of the different stages of C. rogercresseyi to design effective control strategies, improve our understanding of the mechanisms of imidacloprid, and determine the optimal concentrations for control of the parasite.
在智利,目前有 8 种经授权的药物化学合成物可用于控制海虱(Caligus rogercresseyi),即苯甲酸依维菌素、除虫脲、氟苯脲、六氟隆、过氧化氢、溴氰菊酯、氯氰菊酯和氮甲硫磷;然而,这些工具被认为是稀缺的,此外,在以前的研究中也描述过对其中一些化合物失去敏感性的情况。吡虫啉是一种新烟碱类合成杀虫剂,挪威于 2021 年批准使用该杀虫剂,它对控制鲑鱼养殖场中的鲑鳟鱼病毒(Lepeophtheirus salmonis)有很好的效果;但是,智利尚未批准使用该杀虫剂。因此,在本研究中,我们调查了吡虫啉对 C. rogercresseyi 的桡足类和成虫的药效,据我们所知,以前的研究从未调查过这种药效。我们观察到,吡虫啉对这两个阶段的寄生虫都有固定作用;不过,成虫在接触后能够恢复,50%的成虫在接触浓度为百万分之 40 的吡虫啉 24 小时后能够恢复,这表明浓度为百万分之 20 的吡虫啉能够使这种寄生虫 100%死亡。与此相反,桡足虫阶段在 20 ppm 浓度下无法动弹,这表明桡足虫比成虫对吡虫啉更敏感。需要进一步研究了解 C. rogercresseyi 不同阶段的生物反应,以设计有效的控制策略,提高我们对吡虫啉作用机制的认识,并确定控制寄生虫的最佳浓度。
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引用次数: 0
Comparative transcriptomic analysis reveals osmoregulatory mechanisms of juvenile Lateolabrax maculatus responses to long term hypotonic and hypertonic acclimation 转录组比较分析揭示了巨尾蜥幼体对长期低渗和高渗适应性反应的渗透调节机制
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-10 DOI: 10.1016/j.aquaculture.2024.741864
Changlin Li , Longbin Wang , Miaojun Pan , Runze Wang , Haien Zhang , Weidong Li , Xiangli Tian
Salinity limits the abundance and distribution of some fishes in a particular ecological niche. The spotted sea bass (Lateolabrax maculatus), an euryhaline species, exhibits remarkable adaptability to various salinity levels. To elucidate the osmoregulation mechanisms of this species, the growth performance, serum biochemical parameters and gill transcriptomic profiles of the juvenile spotted sea bass subjected to long term hypotonic (0 ppt) and hypertonic environment (30 and 45 ppt), with the isotonic environment (12 ppt) as the control, were investigated. During the 30 days' salinity acclimation, the growth performance of the juvenile sea bass was inhibited significantly (P < 0.05) at hypertonic condition of 45 ppt, while no significant differences were observed among those at 0, 12, and 30 ppt. The serum biochemical indicators of plasma osmolality, concentration of Na+, Cl, glucose, urea, total protein, and ALT and AST activities significantly increased during the hypertonic acclimation. Transcriptome analysis identified 1179, 103, and 443 differentially expressed genes in the 0, 30, and 45 ppt salinity groups, respectively, compared to the isotonic (12 ppt) group. Bioinformatics analysis revealed that signal transduction pathways were promoted during hypotonic acclimation, while energy metabolism pathways were activated during hypertonic acclimation. Protein-protein interaction and WGCNA analysis identified hub genes that may play important functions during acclimation. Among them, protein synthesis related-genes including Rps15, Rps16, Rps9, Rpl4, Rpl15, Rpl30, Rpl31, Rpl13a and Eef2 were down-regulated under hypotonic acclimation, metabolic related-genes including Cs, Idh2, Idh3, Mdh2, Pck1, Pck2, Pdha1 and Ldhb were up-regulated under the hypertonic condition. Rac1 were up-regulated in both conditions. This study provides new insights into the osmoregulatory mechanisms of spotted sea bass in response to long-term hypotonic and hypertonic acclimation.
盐度限制了某些鱼类在特定生态位中的数量和分布。斑点叉尾鲈(Lateolabrax maculatus)是一种极海洋性鱼类,对各种盐度具有显著的适应性。为了阐明该物种的渗透调节机制,研究人员以等渗环境(12 ppt)为对照,对长期处于低渗(0 ppt)和高渗(30 和 45 ppt)环境中的斑海鲈幼鱼的生长表现、血清生化指标和鳃转录组图谱进行了调查。在 30 天的盐度适应过程中,幼鲈的生长性能在 45 ppt 的高渗条件下受到显著抑制(P < 0.05),而在 0、12 和 30 ppt 条件下则无显著差异。在高渗条件下,血浆渗透压、Na+、Cl-、葡萄糖、尿素、总蛋白、ALT 和 AST 活性等血清生化指标明显升高。转录组分析发现,与等渗(12 ppt)组相比,0、30 和 45 ppt 盐度组分别有 1179、103 和 443 个差异表达基因。生物信息学分析表明,信号转导通路在低渗适应过程中得到促进,而能量代谢通路在高渗适应过程中被激活。蛋白质-蛋白质相互作用和 WGCNA 分析发现了可能在适应过程中发挥重要功能的枢纽基因。其中,蛋白质合成相关基因包括Rps15、Rps16、Rps9、Rpl4、Rpl15、Rpl30、Rpl31、Rpl13a和Eef2在低渗条件下下调,代谢相关基因包括Cs、Idh2、Idh3、Mdh2、Pck1、Pck2、Pdha1和Ldhb在高渗条件下上调。Rac1 在两种条件下均上调。这项研究为了解斑点叉尾鲈在长期低渗和高渗条件下的渗透调节机制提供了新的视角。
{"title":"Comparative transcriptomic analysis reveals osmoregulatory mechanisms of juvenile Lateolabrax maculatus responses to long term hypotonic and hypertonic acclimation","authors":"Changlin Li ,&nbsp;Longbin Wang ,&nbsp;Miaojun Pan ,&nbsp;Runze Wang ,&nbsp;Haien Zhang ,&nbsp;Weidong Li ,&nbsp;Xiangli Tian","doi":"10.1016/j.aquaculture.2024.741864","DOIUrl":"10.1016/j.aquaculture.2024.741864","url":null,"abstract":"<div><div>Salinity limits the abundance and distribution of some fishes in a particular ecological niche. The spotted sea bass (<em>Lateolabrax maculatus</em>), an euryhaline species, exhibits remarkable adaptability to various salinity levels. To elucidate the osmoregulation mechanisms of this species, the growth performance, serum biochemical parameters and gill transcriptomic profiles of the juvenile spotted sea bass subjected to long term hypotonic (0 ppt) and hypertonic environment (30 and 45 ppt), with the isotonic environment (12 ppt) as the control, were investigated. During the 30 days' salinity acclimation, the growth performance of the juvenile sea bass was inhibited significantly (<em>P</em> &lt; 0.05) at hypertonic condition of 45 ppt, while no significant differences were observed among those at 0, 12, and 30 ppt. The serum biochemical indicators of plasma osmolality, concentration of Na<sup>+</sup>, Cl<sup>−</sup>, glucose, urea, total protein, and ALT and AST activities significantly increased during the hypertonic acclimation. Transcriptome analysis identified 1179, 103, and 443 differentially expressed genes in the 0, 30, and 45 ppt salinity groups, respectively, compared to the isotonic (12 ppt) group. Bioinformatics analysis revealed that signal transduction pathways were promoted during hypotonic acclimation, while energy metabolism pathways were activated during hypertonic acclimation. Protein-protein interaction and WGCNA analysis identified hub genes that may play important functions during acclimation. Among them, protein synthesis related-genes including <em>Rps15</em>, <em>Rps16</em>, <em>Rps9</em>, <em>Rpl4</em>, <em>Rpl15</em>, <em>Rpl30</em>, <em>Rpl31</em>, <em>Rpl13a</em> and <em>Eef2</em> were down-regulated under hypotonic acclimation, metabolic related-genes including <em>Cs</em>, <em>Idh2</em>, <em>Idh3</em>, <em>Mdh2</em>, <em>Pck1</em>, <em>Pck2</em>, <em>Pdha1</em> and <em>Ldhb</em> were up-regulated under the hypertonic condition. <em>Rac1</em> were up-regulated in both conditions. This study provides new insights into the osmoregulatory mechanisms of spotted sea bass in response to long-term hypotonic and hypertonic acclimation.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"596 ","pages":"Article 741864"},"PeriodicalIF":3.9,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Three-way crossed scallops between Argopecten irradians irradians, A. i. concentricus and A. purpuratus exhibit strong heterosis in growth, survival and temperature tolerance Argopecten irradians irradians、A. i. concentricus 和 A. purpuratus 之间的三元杂交扇贝在生长、存活和耐温性方面表现出很强的异质性
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-10 DOI: 10.1016/j.aquaculture.2024.741866
Jinjing Wang , Oscar Yichen Liu , Min Shen , Guilong Liu , Yinchu Wang , Xia Lu , Xin Xu , Quanchao Wang , Chunde Wang , Junhao Ning
The bay scallops, including the northern subspecies Argopecten irradians irradians (NN) and southern subspecies A. i. concentricus (SS), are economically important cultured bivalves in China. Due to their hermaphroditic nature, the currently farmed bay scallops often suffered from significant inbreeding depression characterized by slow growth, high mortality and low-stress tolerance, especially in the hot summers. In this study, to further improve the production performance and stress resistance of bay scallops, we first conducted a two-way intraspecific hybridization between the two subspecies of the bay scallops (NN and SS), and then hybridized the two-way hybrids with the Peruvian scallop, A. purpuratus (PP) and obtained two three-way hybrids, NSP and SNP. During embryonic and larval stages, both two three-way hybrids exhibited higher fertilization rate, hatching rate and metamorphosis rate, larger initial D-larvae size, and faster embryonic development and larvae growth than those of their parental stocks. The two three-way crossed hybrids displayed excellent heterosis in both growth and survival at larval stage. Both egg origin and mating strategy had significant effects on growth at the larval stage, while the survival rate was mainly dictated by mating strategy. At adult grow-out stage, the heteroses in NSP and SNP cohorts were 12.36 and 19.92 in shell height, 40.37 and 53.27 in whole body weight, and 13.73 and 21.13 in survival rate at harvest, respectively. Moreover, temperature stress tolerance was significantly improved in the three-way crossed scallops. The gonads of most three-way crossed hybrids seemed to be morphologically and functionally females, although the fertility of eggs was very low, suggesting that more energy would be allocated for growth and survival. Notably, the genetic diversity was increased in both three-way hybrids. Therefore, the three-way crossed scallops are potentially precious breeding material for selecting new scallop strains and can be potentially widely cultured along both the southern and northern China coasts.
海湾扇贝,包括北方亚种 Argopecten irradians irradians(NN)和南方亚种 A. i. concentricus(SS),是中国重要的经济养殖双壳贝类。由于其雌雄同体的特性,目前养殖的海湾扇贝经常遭受严重的近亲繁殖抑制,其特点是生长缓慢、死亡率高、抗应激能力低,尤其是在炎热的夏季。在本研究中,为了进一步提高海湾扇贝的生产性能和抗逆性,我们首先在海湾扇贝的两个亚种(NN和SS)之间进行了双向种内杂交,然后将双向杂交种与秘鲁扇贝A. purpuratus(PP)杂交,得到了两个三向杂交种NSP和SNP。与亲本相比,两个三向杂交种在胚胎期和幼体期均表现出较高的受精率、孵化率和变态率,初始D型幼体较大,胚胎发育和幼体生长速度较快。两个三系杂交种在幼虫期的生长和存活率方面都表现出很好的异质性。卵源和交配策略对幼虫期的生长均有显著影响,而成活率则主要受交配策略的影响。在成虫生长阶段,NSP 和 SNP 组群在壳高方面的异质性分别为 12.36 和 19.92,在全体重方面的异质性分别为 40.37 和 53.27,在收获成活率方面的异质性分别为 13.73 和 21.13。此外,三系杂交扇贝对温度胁迫的耐受性明显提高。虽然卵的受精率很低,但大多数三向杂交种的性腺在形态和功能上似乎都是雌性的,这表明更多的能量将用于生长和存活。值得注意的是,两种三向杂交种的遗传多样性都有所增加。因此,三向杂交扇贝可能是选育扇贝新品系的珍贵育种材料,可在中国南部和北部沿海广泛养殖。
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引用次数: 0
Stress and immune responses in rainbow trout (Oncorhynchus mykiss) treated by a low dose vaccine injection 注射低剂量疫苗后虹鳟鱼(Oncorhynchus mykiss)的应激和免疫反应
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-10 DOI: 10.1016/j.aquaculture.2024.741871
X.H. Liu , M. Teles , A. Tvarijonaviciute , I. Brandts , Y.G. Zhang , L. Tort
The animal stress response is initiated by the locus coeruleus-noradrenaline (LC-NE) system and followed by an activation of hypothalamic-pituitary-interrenal (HPI) axis. Pathogens represent a common physiological stressor, and vaccination is the most used method to prevent bursts of infectious disease in aquaculture. Moreover, vaccination is an alternative way to study the physiological changes corresponding to an infectious process. In the present study, with the aim of investigating the interaction between neuroendocrine and immune responses during the initial stage of natural pathogen infection, rainbow trout (Oncorhynchus mykiss) were injected with inactivated Vibrio anguillarum bacterin (100 μL). Fish were sampled at different time-points: 1, 6, 24, and 72 h post-infection (hpi), and blood, brain, pituitary, head kidney, spleen, intestine, and liver were sampled. Biochemical parameters were analyzed in plasma (e.g., concentrations of corticotropin-releasing hormone (CRH) and cortisol), and the mRNA levels of genes involved in catecholamine synthesis and metabolism, hypothalamic-pituitary-interrenal (HPI) axis, and immune responses were assessed in the sampled tissues. Results showed that stress parameters, including plasma glucose, protein level, cortisol, and expression of pomcα in the pituitary were significantly changed at 1hpi (p < 0.05), while plasma IL1β levels and expression of immune related genes, including il1β, il8, il6, were mainly significantly changed at the latter stages (24 hpi, 72 hpi) in the spleen and brain (p < 0.05). This indicates a first stress response with a latter immune response. Significant changes in the expression of mao, pnmt and dbh in the brain were also observed, implying firstly the effective local and systemic role of catecholamines and secondly interactivities between the LC-NE system and HPI in the maintenance of stress response.
动物应激反应由脑皮质-去甲肾上腺素(LC-NE)系统启动,随后激活下丘脑-垂体-肾上腺(HPI)轴。病原体是一种常见的生理应激源,接种疫苗是水产养殖中预防传染病爆发的最常用方法。此外,接种疫苗也是研究感染过程相应生理变化的另一种方法。在本研究中,为了研究自然病原体感染初期神经内分泌和免疫反应之间的相互作用,给虹鳟鱼(Oncorhynchus mykiss)注射了灭活的鳗鲡弧菌细菌素(100 μL)。在不同的时间点对鱼进行采样:分别在感染后 1、6、24 和 72 小时(hpi)采集血液、脑、垂体、头肾、脾、肠和肝脏样本。对血浆中的生化指标(如促肾上腺皮质激素释放激素(CRH)和皮质醇的浓度)进行了分析,并对取样组织中涉及儿茶酚胺合成和代谢、下丘脑-垂体-肾上腺(HPI)轴和免疫反应的基因的 mRNA 水平进行了评估。结果表明,应激参数,包括血浆葡萄糖、蛋白质水平、皮质醇和垂体中 pomcα 的表达在 1 hpi 有显著变化(p < 0.05),而血浆 IL1β 水平和免疫相关基因(包括 il1β、il8、il6)的表达主要在后期(24 hpi、72 hpi)在脾脏和大脑中有显著变化(p < 0.05)。这表明先有应激反应,后有免疫反应。在大脑中也观察到 mao、pnmt 和 dbh 表达的显著变化,这首先意味着儿茶酚胺在局部和系统中的有效作用,其次意味着 LC-NE 系统和 HPI 在维持应激反应中的相互作用。
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引用次数: 0
Diet shift and allometric growth in the early development Odontobutis potamophila 食性转变和钝齿鳗早期发育的异速生长
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-10 DOI: 10.1016/j.aquaculture.2024.741880
Miao Xiang , Kun Xiao , Hongliang Xu , Bo Li , Hongxi Guo , Zhanhu Yang , Chuankun Zhu , Man Wang , Jie Wang , Wei Xin , Songguang Xie , Xiaoming Zhu , Lei Zhang
The viability of fish during their early developmental stages is pivotal for population recruitment, a period during which individuals are frequently confronted with significant risks of starvation. There is limited knowledge regarding Odontobutis potamophila food selectivity before its transition to feeding on fish. Thus, this study aimed to elucidate its prey composition and diet shift from 0 to 22 days after hatching. We also examined early allometric growth patterns to confirm whether there was a correlation between the rapid onset of exogenous feeding in juvenile carnivorous fish and the earlier appearance of inflection points in various morphological traits. Cluster analysis showed a gradual shift in prey composition from nauplii to predominantly cladocerans and copepods as individuals grew. This adaptive shift in diet revealed a mechanism whereby the larger the individual, the larger the size of the bait ingested, and the faster the growth rate. The results of the allometric growth study demonstrated that O. potamophila emerged from the membrane with a total length of 6.53 ± 0.51 mm and a body weight of 1.93 ± 0.56 mg. The positive allometric growth of body weight relative to total length provides the required energy for organ development and onset of the juvenile stage. Feeding commences at one day post-hatching, with the yolk sac fully depleted by day 6, thereby facilitating the transition into the exogenous nutritional phase as early as possible. The initial manifestation of certain morphological growth inflection points occurred predominantly around 10 days of age, concomitant with the advent of a shift in feeding habits. The prey composition, diet shifts, and growth patterns of early individuals with equilibrium strategy, represented by O. potamophila, have important implications for improving the survival of early life stages, thereby providing new strategies for population replenishment.
鱼类在早期发育阶段的生存能力对种群数量的增加至关重要,在此期间,鱼类个体经常面临巨大的饥饿风险。目前,人们对锅盖鱼过渡到以鱼类为食之前的食物选择性了解有限。因此,本研究旨在阐明其猎物组成以及孵化后 0-22 天内的食性转变。我们还研究了早期的异速生长模式,以确认幼年肉食性鱼类快速开始外源摄食与各种形态特征的拐点提前出现之间是否存在相关性。聚类分析显示,随着个体的成长,猎物组成从稚鱼逐渐转变为主要为衣壳纲和桡足类。这种食性的适应性转变揭示了一种机制,即个体越大,摄取的饵料体积越大,生长速度越快。异速生长研究结果表明,O. potamophila出膜时的总长度为 6.53 ± 0.51 毫米,体重为 1.93 ± 0.56 毫克。体重相对于总长度的正异速增长为器官发育和幼体阶段的开始提供了所需的能量。孵化后一天开始喂食,卵黄囊在第 6 天完全耗尽,从而促进尽早过渡到外源营养阶段。某些形态生长拐点的最初表现主要出现在 10 日龄左右,与此同时出现了摄食习惯的转变。以壶瓶鱼为代表的具有平衡策略的早期个体的猎物组成、食性转变和生长模式对提高早期生命阶段的存活率具有重要意义,从而为种群补充提供了新的策略。
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引用次数: 0
Rapid, DNA extraction-free, detection and quantification of Escherichia coli in mussels by Most Probable Number colorimetric Loop-mediated isothermal amplification 通过最可能数比色环介导等温扩增技术,快速、免 DNA 提取、检测和量化贻贝中的大肠埃希氏菌
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-10 DOI: 10.1016/j.aquaculture.2024.741873
Lucía García-Sanmartín , Rosalía Creo-Menéndez , Juan José Rodríguez-Herrera , Alejandro Garrido-Maestu
Escherichia coli is a very well-known microorganism typically selected as indicator of fecal contamination in foods and water. The reference method for the quantification of E. coli in live mollusks relies on Most Probable Number (MPN) followed by tube confirmation on a chromogenic medium, TBX, ISO 16649-3. Even though reliable, this approach needs a minimum of two days to be completed, making it not ideal for short shelve life foods such as seafood. In the current study the conventional TBX confirmation was replaced by a novel, colorimetric Loop-mediated isothermal amplification (LAMP) assay. The MPN-LAMP method allowed to reduce by half the turnaround time of the method, 24 vs 48 h, when compared to the standard MPN-TBX. In addition to this, given the robustness of this technique to conventional inhibitory compounds, it was possible to directly add the presumptive positive tube suspension of the tube to the reaction vessel, without DNA extraction, and interpret the results in 30–35 min, after the MPN step. This approach allowed to simplify the workflow, reduce hands-on work, and costs associated to the assay. By analyzing a total of 39 mussel samples spiked with concentration ranges from <0.42–76.82 MPN/ g, minor differences in the results were observed among both confirmatory approaches, not being these statistically significant.
大肠杆菌是一种非常著名的微生物,通常被选为食品和水粪便污染的指标。活体软体动物中大肠杆菌的定量参考方法依赖于最大可能数 (MPN),然后在发色培养基 TBX(ISO 16649-3)上进行试管确认。这种方法虽然可靠,但至少需要两天才能完成,因此不适合海鲜等货架期短的食品。在本研究中,传统的 TBX 确认方法被一种新颖的比色环介导等温扩增(LAMP)检测方法所取代。与标准的 MPN-TBX 方法相比,MPN-LAMP 方法的周转时间缩短了一半(24 小时对 48 小时)。除此之外,鉴于该技术对传统抑制性化合物的稳健性,可以直接将推定的阳性试管悬浮液加入反应容器中,无需提取 DNA,在 MPN 步骤后的 30-35 分钟内即可解释结果。这种方法简化了工作流程,减少了实际操作,降低了检测成本。通过分析总计 39 个贻贝样本,其浓度范围为 0.42-76.82 MPN/g,两种确证方法的结果略有不同,但无统计学意义。
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引用次数: 0
Corrigendum to “Assessment of refractory dissolved organic carbon in sediments of marine culture ponds: An example of two culture systems for Penaeus vannamei” [Aquaculture Volume 593, 15 December 2024, 741368] 海水养殖池塘沉积物中难溶有机碳的评估:以万年青的两种养殖系统为例"[《水产养殖》第593卷,2024年12月15日,741368]
IF 3.9 1区 农林科学 Q1 FISHERIES Pub Date : 2024-11-09 DOI: 10.1016/j.aquaculture.2024.741832
Chenxiao Huang , Teng Jiang , Boshan Zhu , Dapeng Liu , Hongwei Shan , Fang Wang
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引用次数: 0
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