Aquaculture最新文献

{"title":"Exposure to glyphosate causes hypoxia in crustaceans by targeting hemocyanin","authors":"Rui-Geng Niu,&nbsp;Cheng-Ming Yin,&nbsp;Jia-Yu Si,&nbsp;Meng-Di Tang,&nbsp;Jiang-Feng Lan","doi":"10.1016/j.aquaculture.2025.742314","DOIUrl":"10.1016/j.aquaculture.2025.742314","url":null,"abstract":"<div><div>Glyphosate is the most widely used herbicide in the world, and it has been detected in aquatic environments. However, the toxicity mechanism of glyphosate is not fully understood, especially its target in animals. This study investigates the impact of glyphosate exposure on <em>Procambarus clarkii</em> (crayfish) and explores the underlying biological mechanisms. The results demonstrate that glyphosate exposure significantly alters crayfish behavior and causes histopathological damage. Further analysis revealed that glyphosate exposure induces hypoxia and notably reduces the expression of hemocyanin (HMC). Glyphosate was found to directly bind to hemocyanin, potentially reducing its oxygen-carrying capacity. Glyphosate exposure activated the MAPK-AP1 signaling pathway, which subsequently modulated the transcription of hemocyanin subunits through the transcription factor AP1, likely playing a role in the regulation of hemocyanin production. Glyphosate exposure also disrupted the microbial composition of crayfish hemolymph, affecting both microbial load and diversity. This study provides novel insights into the complex toxicological effects of glyphosate on crayfish, revealing its interference with hemocyanin function and microbiome composition, and underscores the need for further research into its broader ecological consequences.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"602 ","pages":"Article 742314"},"PeriodicalIF":3.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA sequencing reveals the heterogeneity of hepatopancreas cells and their association with gonadal development in the red swamp crayfish Procambarus clarkii","authors":"Lin Du ,&nbsp;Yuning Hu ,&nbsp;Siyu Lu ,&nbsp;Xuguang Li ,&nbsp;Jiajia Li ,&nbsp;Hai Lin ,&nbsp;Jiaxin Yang ,&nbsp;Yu Xu ,&nbsp;Zhiqiang Xu","doi":"10.1016/j.aquaculture.2025.742311","DOIUrl":"10.1016/j.aquaculture.2025.742311","url":null,"abstract":"<div><div>The hepatopancreas plays a crucial role in synthesizing yolk substances during ovarian development in crustaceans, but the diverse types of hepatopancreas cells and their specific roles in ovarian development in <em>Procambarus clarkii</em> remain unclear. During ovarian development, the hepatopancreatic index increased from 6.5 % to 8.14 % between stages I and IV, then decreased to 4.35 % by stage VI. To reveal the heterogeneity of hepatopancreas cells and their association with ovarian development in <em>P. clarkii</em>, single-cell transcriptome sequencing was performed on the hepatopancreas from three distinct phases of ovarian development: the large growth phase (stage III), the mature phase (stage V), and the recovery phase (stage VI), analyzing a total of 16,626 cells. The cells were classified into five types: R-cells, <em>E</em>-cells, B-cells, F-cells, and M-cells. R-cells had the highest proportion (52.86 %), followed by E-cells (24.68 %). The proportion of R-cells varied from 65.10 % at stage III to 26.42 % at stage V, and 50.62 % at stage VI. Pseudo-time analysis indicated that <em>E</em>-cells were the first to differentiate, gradually giving rise to other cell types. Key pathways related to ovarian development were enriched in R- and <em>E</em>-cells, such as “oxidative phosphorythlation”, “protein processing in the endoplasmic reticulum” and “antigen processing and presentation”. By screening for differentially expressed genes within these pathways, potential functional marker genes linked to ovarian development in the hepatopancreas were identified and validated through qPCR, including <em>Lysophosphatidylcholine Acyltransferase</em> and <em>Vitelline Membrane Outer Layer Protein I</em>. These findings may provide insights into the molecular regulatory mechanisms governing ovarian development in crustaceans.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742311"},"PeriodicalIF":3.9,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal transcriptome analysis provides molecular insights into night-time molting of juvenile mud crab (Scylla paramamosain)","authors":"Shujian Chen ,&nbsp;Jiahao Liu ,&nbsp;Ce Shi ,&nbsp;Herve Migaud ,&nbsp;Xuelin Zhao ,&nbsp;Qingyang Wu ,&nbsp;Yangfang Ye ,&nbsp;Ronghua Li ,&nbsp;Weiwei Song ,&nbsp;Changkao Mu ,&nbsp;Chunlin Wang","doi":"10.1016/j.aquaculture.2025.742312","DOIUrl":"10.1016/j.aquaculture.2025.742312","url":null,"abstract":"<div><div>Molting is an important physiological trait in crustaceans, through which they can realize metamorphosis, growth, and reproduction. In this study, the diurnal differences in molting of <em>Scylla paramamosain</em> were investigated with a 9-week experiment, and the temporal transcriptome was introduced to investigate the potential roles of circadian clock in entraining the molting rhythm. The results conducted from 6 molting cycles showed that crabs exposed to a simulated natural photoperiod (SNP, 12 L:12D) molted primarily during the nocturnal phase (61.54 % - 82.50 %), which is significantly higher than daytime (26.82 ± 7.20 % vs. 73.18 ± 7.20 %) (<em>P</em> &lt; 0.05). The melatonin and ecdysone content in the haemolymph of crabs reared under SNP conditions exhibited obvious daily rhythms (<em>P</em> &lt; 0.05); phase analysis showed that the melatonin content reached its peak at ZT0/ZT24, and the ecdysone content peaked during the photophase. Temporal transcriptome analysis from crabs exposed to natural photoperiod revealed that 4296 expressed genes (38.34 %) displayed significant daily rhythmicity. GO and KEGG enrichment analysis showed that rhythmic expressed genes were mainly involved in catalytic activity on nucleic acid, ribosome biogenesis, mitochondrial protein-containing complex, protein acetylation, and pathways such as mRNA biogenesis, ribosome biogenesis, spliceosome, DNA repair, and cell cycle. In addition, diurnal expressed genes (peak at ZT0/ZT24-ZT8) mainly included methyltransferase activity, ATP hydrolysis activity, chromatin binding, and protein methyltransferase activity terms. Nocturnal expressed genes (peak at ZT12-ZT20) were primarily involved in spliceosome, arginine biosynthesis, circadian rhythm, amino sugar and nucleotide sugar metabolism, and nitrogen metabolism, which providing insight into the circadian entrainment of crustacean molting. Moreover, the expression of circadian clock-related genes (<em>cry1</em>, <em>cry2</em>, <em>period</em>, <em>clock</em>, and <em>cycle</em>), 20E signaling pathway-related genes (<em>rxr</em>, <em>hr4</em>, <em>ftzf1</em>), and chitin metabolism-related genes (<em>g6pi</em>, <em>gna</em>, <em>pagm</em>, <em>pgm</em>, <em>chit</em>, and <em>nag</em>) displayed significant diurnal oscillations (<em>P</em> &lt; 0.05). Overall, the present research confirmed that the molting of <em>S. paramamosain</em> under natural photoperiod display a daily rhythm, that may be entrained by the rhythmic expression of genes involved in circadian clock, arginine biosynthesis and chitin metabolism pathways.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742312"},"PeriodicalIF":3.9,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raising temperature at lower heating rate is an effective way to prevent Micropterus salmoides rhabdovirus disease","authors":"Runzhen He ,&nbsp;Xiaoming Chen ,&nbsp;Ningyu Zhu,&nbsp;Qianrong Liang,&nbsp;Gaohua Yao,&nbsp;Quanquan Tian,&nbsp;Fan Zhou,&nbsp;Xueyan Ding","doi":"10.1016/j.aquaculture.2025.742287","DOIUrl":"10.1016/j.aquaculture.2025.742287","url":null,"abstract":"<div><div><em>Micropterus salmoides</em> rhabdovirus (MSRV), is a single-stranded RNA virus that results in mass morbidity and mortality in largemouth bass (<em>Micropterus salmoides)</em>, leading to severe economic losses for the largemouth bass aquaculture. To control outbreaks of MSRV disease, we investigated the influence of water temperature on the infectivity of MSRV and the resistance of largemouth bass to MSRV. In addition, we explored whether higher water temperature is an effective way to prevent outbreaks of MSRV disease. The rhabdovirus was added to three sterile pond water samples and incubated at 10 °C, 20 °C, and 30 °C for 14 days. The viral load of each pond water sample was calculated by RT-qPCR, and the infectivity was evaluated by inoculating grass carp ovary cells with samples from each pond water. In addition, juvenile fish were challenged with the MSRV at different water temperatures (15 °C, 20 °C, 25 °C and 30 °C). After the challenged, these juveniles were transferred to ponds with a water temperature ranging from 25 °C to 30 °C with different heating rates. The results showed that the number of viral copies decreased as temperature increased, and the viral copies in the 10 °C, 20 °C, and 30 °C groups were lower than the detection threshold at 11, 7, and 3 dpi, respectively. The viral infectivity was significantly reduced in the 30 °C group, with infectivity lost at 5, 5, and 1 dpi in the 10 °C, 20 °C and 30 °C group, respectively. In addition, the fish mortality rates were 56.67 % ± 10.41 %, 73.33 % ± 5.77 %, 60 % ± 10 % and 5 % ± 5 % at 15 °C, 20 °C, 25 °C and 30 °C, respectively. The mortality rate of the juvenile fish decreased from 59.52 % ± 4.12 % to 14.28 % ± 7.14 %, and the viral copies decreased significantly in the liver, spleen, and kidney of the T2 group that was exposed to a temperature increase of 0.5 °C every 1 h from 25 °C to 30 °C. In conclusion, higher temperatures (30 °C) can reduce MSRV infectivity, and a low heating rate (0.5 °C every 1 h) exerts a synergistic antioxidant effect by enhancing SOD and CAT activity and improving host immunity, ultimately helping prevent outbreaks of MSRV disease in largemouth bass. These results can be applied to improve biosecurity practices in largemouth bass farms to control the outbreaks of MSRV disease.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742287"},"PeriodicalIF":3.9,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic dissection of high-temperature resistance in hybrid oysters (Crassostrea gigas ♀ × C. angulata ♂) using SNP- and InDel-GWAS based on whole-genome resequencing","authors":"Shilong Dou ,&nbsp;Gaowei Jiang ,&nbsp;Ben Yang ,&nbsp;Lingling Sun ,&nbsp;Qi Li","doi":"10.1016/j.aquaculture.2025.742310","DOIUrl":"10.1016/j.aquaculture.2025.742310","url":null,"abstract":"<div><div>Global warming has significantly impacted the aquaculture industry, creating an urgent need for genetic analysis of traits related to heat tolerance. Oysters are economically vital shellfish, but high summer temperatures cause severe mortality, challenging global aquaculture. Understanding the genetic basis of high-temperature tolerance in oysters and advancing genetic improvement strategies are crucial for rapidly developing heat-tolerant strains. The hybrid lines (GA) between the Pacific oysters (<em>Crassostrea gigas</em>) ♀ and the Fujian oysters (<em>Crassostrea angulata</em>) <strong>♂</strong>, as well as the self-cross progeny of these hybrids, have exhibited significant heat tolerance and notable phenotypic variation, making them excellent materials for genetic analysis. In this study, GA oysters that had undergone high-temperature selection for four generations were used as samples and subjected to heat stress experiments at their semi-lethal temperature (LT₅₀, 42 °C). Genotyping through whole-genome resequencing identified 9,692,180 single nucleotide polymorphisms (SNPs) and 3,883,291 insertions/deletions (InDels). Genome-wide association studies (GWAS) revealed 17 SNPs and 14 InDels significantly associated with high-temperature resistance, identifying 25 candidate genes through gene annotation. Gene Ontology (GO) enrichment analysis indicated that the candidate genes are associated with key biological processes such as phosphorylation, centrosome, kinase activity, and molecular adaptor activity. The focused 12 candidate genes were validated by RT-PCR in the sensitive and tolerant groups, identifying four essential regulatory genes (<em>TIMP3</em>, <em>THH</em>, <em>SLC38A11</em>, <em>TRIM2</em>) associated with protein synthesis, degradation, cell stability and other physiological processes related to heat tolerance in oysters. These findings provide valuable insights for marker-assisted selection (MAS) and genomic selection (GS) breeding of heat-tolerant oysters.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742310"},"PeriodicalIF":3.9,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biologger attachment and retention in channel catfish (Ictalurus punctatus)","authors":"Melanie R. Boudreau ,&nbsp;Shelby Rainbolt ,&nbsp;Hunter Lister ,&nbsp;Ali Diamond ,&nbsp;Alison M. Lee ,&nbsp;Peter J. Allen","doi":"10.1016/j.aquaculture.2025.742281","DOIUrl":"10.1016/j.aquaculture.2025.742281","url":null,"abstract":"<div><div>Accelerometers capture detailed, real-time information over extended periods, offering perspective on the movements, physiology, and behavior of free-roaming animals in varying environments. Within economically significant aquaculture systems, acceleration can be used as a quantitative surrogate for metabolism and contribute data for energy models and fish welfare indices. For channel catfish (<em>Ictalurus punctatus</em>), the most commonly cultured fish in the United States, little is known about how various attachment types (either external or internal) might impact the acceleration-metabolism relationship or, for internally attached tags, how well fish can retain tags given the species' propensity to expel foreign objects. We placed accelerometers either internally or externally in channel catfish and took simultaneous measurements of acceleration and metabolic rate over increasing water velocities in a flume. For internal attachments, we also examined retention time of accelerometer units of varying shapes that had similar volumes (the manufacturer's original shape that was rectangular and designed for hydrodynamics while attached to an outer fin, a flattened cylindrical shape, and an oval shape). We found a distinct relationship between metabolism and acceleration in relation to water velocity given the location of accelerometer placement, with a 23 % lower metabolic rate and 43 % lower acceleration for fish with internal accelerometers compared to external accelerometers. This translated to a lower metabolic-ODBA relationship for internal accelerometers which is likely indicative of an effect of drag and tail undulations for units that were externally attached. When looking at retention, we found that the manufacturer's accelerometer shape drastically increased the chances of mortality over the course of 15 weeks (71 % mortality compared to 20 % for the oval shape and 8 % for the flattened cylinder). The odds of retention dropped by 66 %, 72 %, and 67 % for the oval, flattened cylindrical, and manufacturer's shapes over 10 weeks, respectively. Notably, channel catfish had a remarkable propensity to expel tags implanted into the peritoneal cavity, with no accelerometer tags retained after 15 weeks, a propensity which has been documented in other studies on catfish and in other species such as rainbow trout (<em>Oncorhynchus mykiss</em>). We recommend that users determine acceleration-physiology relationships in relation to tag placement before broad scale use and that flattened cylindrical shapes are used for internal tags. This work further emphasizes the need for groundwork for biologging technology before it is used as a tool for improving management of aquaculture operations.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742281"},"PeriodicalIF":3.9,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing flow-through aquaculture system monitoring: A comparative study of machine learning algorithms for missing-data imputation","authors":"Hakjong Shin,&nbsp;Taehyun Park,&nbsp;Seng-Kyoun Jo,&nbsp;Jae Young Jung","doi":"10.1016/j.aquaculture.2025.742303","DOIUrl":"10.1016/j.aquaculture.2025.742303","url":null,"abstract":"<div><div>This study evaluated flow-through aquaculture systems by assessing the efficiency of various machine learning algorithms for imputing missing water-quality data, including dissolved oxygen, water temperature, pH, and salinity. Artificial missing data were generated based on real-world missing data mechanisms, and a comprehensive statistical analysis of the data characteristics was conducted to identify suitable imputation methods. Results showed that basic imputation methods like linear interpolation, often insufficient for datasets with high variability and non-linear relationships, performed well for certain data distributions, particularly for salinity and pH data with high kurtosis and symmetric distributions. However, advanced machine learning-based imputation techniques, especially TimesNet, consistently outperformed other methods in handling complex and variable patterns in the water-quality data. This study underscores the importance of selecting appropriate imputation methods based on data properties to enhance environmental monitoring systems in aquaculture and improve operational efficiency and sustainability.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742303"},"PeriodicalIF":3.9,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chlorpyrifos stress on the Eriocheir sinensis suffering from Metschnikowia bicuspidata infection","authors":"Jiaming Dong ,&nbsp;Yuanyuan Tan ,&nbsp;Chengcheng Feng ,&nbsp;Qingbiao Hu ,&nbsp;Yanhong Zhao ,&nbsp;Jie Bao ,&nbsp;Hongbo Jiang","doi":"10.1016/j.aquaculture.2025.742293","DOIUrl":"10.1016/j.aquaculture.2025.742293","url":null,"abstract":"<div><div>This study investigated the effects of chlorpyrifos (CPF) stress on the prevalence of “milky disease” caused by <em>Metschnikowia bicuspidata</em> in Chinese mitten crabs (<em>Eriocheir sinensis</em>), a key issue affecting crab aquaculture. The 96 h LC₅₀ of CPF was determined to be 0.568 mg/L. The experimental setup included a control group (no CPF exposure) and three CPF-treated groups exposed to low (0.0189 mg/L), medium (0.0568 mg/L), and high (0.189 mg/L) CPF concentrations. Over a 7-day period, histomorphological analyses, immune enzyme activity assessments, and gene expression evaluations were conducted. CPF exposure resulted in appendage rigidity, neurological symptoms, and increased severity of “milky disease” and hepatopancreatic damage. Yeast proliferation on day 7 increased with CPF concentration and was significantly higher in CPF-exposed crabs when low doses of <em>M. bicuspidata</em> were injected. Crabs subjected to higher CPF doses and infection showed elevated mortality rates. Physiological tests revealed CPF exposure reduced antioxidant defenses by inhibiting acid and alkaline phosphatase activities and altering phenol oxidase, malondialdehyde, lysozyme, and glutathione peroxidase levels. Transcriptome analysis showed CPF exposure impaired lipid metabolism, energy supply, and oxygen transport pathways, weakening immune defense mechanisms. These results demonstrate that CPF exposure reduces immune function and antioxidant capacity, thereby accelerating <em>M. bicuspidata</em> proliferation and worsening disease outcomes. This study offers critical insights into the role of environmental stressors in the spread of “milky disease,” providing valuable guidance for sustainable crab aquaculture practices.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742293"},"PeriodicalIF":3.9,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of egg quality parameters to evaluate the effect of a diet supplemented with algae and antioxidants in turbot (Scophthalmus maximus)","authors":"S. Ramos-Júdez ,&nbsp;E. Fatsini ,&nbsp;C. Marrero-Alemán ,&nbsp;C. García-Pichel ,&nbsp;P. Parente ,&nbsp;D. Medina ,&nbsp;C. Castro ,&nbsp;E. Cabrita ,&nbsp;C. C.V. Oliveira","doi":"10.1016/j.aquaculture.2025.742306","DOIUrl":"10.1016/j.aquaculture.2025.742306","url":null,"abstract":"<div><div>Enhancing egg quality can be achieved by improving breeders' diet through the antioxidant content, as oxidative stress could adversely affect egg quality. Micro- and macroalgae species are natural sources of antioxidants and other essential nutrients that can be incorporated in fish feeds. In this study the quality of <em>Scophthalmus maximus</em> eggs were compared between breeders fed a commercial (non-supplemented) diet and those fed a diet enriched with 5 % <em>Arthrospira platensis</em> and 1 % of the iodine-rich <em>Laminaria digitata</em>, further fortified with antioxidants (astaxanthin, vit. C and E) (supplemented diet). Several egg parameters were evaluated. Hierarchical clustering of all the egg batches grouped them into two main categories: higher (≥ 50 % buoyancy) and lower-quality eggs (≤ 30 % buoyancy). The expression of transcripts related to egg quality (<em>ctsz</em>, <em>ccna2</em>), oxidative response (<em>nrf2</em>, <em>cat</em>), and apoptosis (<em>bax</em>, <em>casp3a</em>) was also measured in batches categorized by quality, as well as in higher-quality batches from females fed the commercial versus supplemented diet. Eggs of higher quality (greater buoyancy), regardless of females' diet, had significantly higher total antioxidant status (TAS) levels (<em>P</em> &lt; 0.05), suggesting TAS as an egg quality marker. The expression of <em>ccna2</em> was upregulated, while <em>ctsz</em> showed almost no expression in higher-quality eggs but was down-regulated in lower-quality eggs, highlighting their potential as markers of egg quality in turbot. Higher-quality eggs from females fed the supplemented diet exhibited higher TAS, lower superoxide dismutase activity, and an upregulation of <em>nrf2</em> compared to higher-quality eggs from non-supplemented females. This suggests a more efficient cellular mechanism to restore oxidative homeostasis. Supplementing the diet increased the likelihood of achieving ≥80 % buoyant eggs and overall cumulative egg production, contributing to more effective and sustainable turbot farming.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742306"},"PeriodicalIF":3.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of dual specificity phosphatase genes (DUSP) in silver pomfret (Pampus argenteus): Protective effects of DUSP1 and DUSP5 against cold stress","authors":"Mengke Tang ,&nbsp;Man Zhang ,&nbsp;Jiabao Hu ,&nbsp;Yubei Qiu ,&nbsp;Youyi Zhang ,&nbsp;Yaya Li ,&nbsp;Yuanbo Li ,&nbsp;Qinqin Dai ,&nbsp;Shangliang Xu ,&nbsp;Lu Zhang ,&nbsp;Zi Yuan ,&nbsp;Yajun Wang","doi":"10.1016/j.aquaculture.2025.742307","DOIUrl":"10.1016/j.aquaculture.2025.742307","url":null,"abstract":"<div><div>Silver pomfret is an important economic marine fish, because of its inability to overwinter in natural water of artificial culture process, the development of silver pomfret industrial culture is limited. The water was cooled from 19 °C to 7 °C, then rewarmed to 15 °C. Tissue structure and mitochondria damage of liver, gill and muscle were observed under cold stress. The gill showed swelling and vacuolization, liver showed swelling and cell degeneration, and muscle showed fiber rupture under cold stress in silver pomfret. Besides, the apoptosis of cells in the gill is most pronounced. Mitochondrial structure showed significant damage in liver, gill and muscle at 7 °C. Dual-specificity phosphatases (Dusps) play crucial regulatory roles in cellular responses to various stresses. Therefore, it is necessary to understand the physiological changes and the <em>dusp</em>s of silver pomfret in response to cold stress. To investigate whether <em>dusp</em> can reduce cold stress damage, we identified twenty-nine members of the <em>dusp</em>s family according to the genome database of silver pomfret. Phylogenetic and structural analyses suggest some conservation in the <em>dusp</em>s family. And <em>dusp1</em>, <em>dusp2</em>, <em>dusp5</em>, <em>dusp6</em>, <em>dusp14a</em> and <em>dusp22a</em> were found highly expressed in different tissues by analyzing the cold transcriptome. The liver cell of silver pomfret line was subjected to overexpression of <em>dusp1</em> and <em>dusp5</em>. The expression of downstream genes in the MAPK signaling pathway (<em>jnk</em>, <em>erk</em> and <em>p38</em>) were downregulated after the overexpression of <em>dusp1</em> and <em>dusp5</em>. And the expression of apoptosis-related genes (<em>p53</em>, <em>caspase3</em> and <em>caspase8</em>) and stress-related genes (<em>sod3</em>, <em>atf4</em> and <em>atf6</em>) were downregulated after the overexpression of <em>dusp1</em>. After the overexpression of <em>dusp5</em> the expression of apoptosis-related genes (<em>bcl2</em> and <em>bax</em>), stress-related genes (<em>sod2</em>, <em>sod3</em>, <em>chop</em>, <em>perk</em>, <em>atf4</em> and <em>atf6</em>) and mitochondria fusion genes (<em>mfn</em> and <em>letm</em>) were downregulated. The results suggested that <em>dusp1</em> and <em>dusp5</em> may help silver pomfret adapt to cold stress by reducing cell apoptosis and stress reaction. This study helps understand the damage by cold stress, the response of <em>dusp</em>s family to cold stress, and the regulatory role of <em>dusp1</em> and <em>dusp5</em> in cold stress, that also provides theoretical basis for low-temperature tolerance breeding of silver pomfret.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742307"},"PeriodicalIF":3.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}