Aquaculture最新文献_第2页

Assessing environmental innovation capacity and needs of mussel farmers in Italy: A mixed-method approach 评估意大利贻贝养殖户的环境创新能力和需求：一种混合方法

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-22 DOI: 10.1016/j.aquaculture.2026.743693

Elena Pagliarino , Greta Falavigna , Sara Bonanomi , Giuseppe Prioli , Luca Bolognini

Mussel farming represents a key segment of Italian aquaculture but faces significant economic and environmental sustainability challenges. This study examines the environmental innovation capacity and needs of mussel producers in Italy's Campania region through a mixed-method approach combining Data Envelopment Analysis (DEA) with qualitative insights from farmers, experts, and research and development (R&D) projects literature. DEA was applied to 24 mussel farms using financial data from 2016 to 2019 to calculate efficiency as a reliable proxy for innovation capacity, revealing low average efficiency (mean: 0.236) and high variability. The regression analysis revealed that firm liquidity and profitability emerged as significant determinants of efficiency. A structured questionnaire survey with 14 producers showed strong interest in expanding farming areas, modernizing equipment, and adopting environmental innovations that are also economically beneficial, such as waste management and energy saving solutions. Producers demonstrated limited awareness of the environmental impact of their activity as well as the need for financial support and technical training. The review of R&D projects indicated that environmental innovation is mainly understood in terms of replacing plastic with biodegradable materials in farming equipment. However, emerging technologies are not yet available, whereas waste management solutions appear more feasible. Interviews and discussions with experts further highlighted structural weaknesses in the sector, undermining both development and propensity to innovate. Findings suggest that fostering environmental innovation in mussel farming requires integrated strategies that combine policy inducement mechanisms, including financial incentives, training, quality control, and promotion of production standards, with continued experimentation in technological change and a deep understanding of the specific socio-environmental context in which innovation must be applied.

贻贝养殖是意大利水产养殖的重要组成部分，但面临着重大的经济和环境可持续性挑战。本研究采用数据包络分析（DEA）与来自农民、专家和研发项目文献的定性见解相结合的混合方法，考察了意大利坎帕尼亚地区贻贝生产商的环境创新能力和需求。利用2016年至2019年的财务数据，将DEA应用于24个贻贝养殖场，以计算效率作为创新能力的可靠代理，结果显示平均效率低（平均值：0.236）且变动性高。回归分析显示，企业的流动性和盈利能力成为效率的重要决定因素。对14个生产者进行的结构化问卷调查显示，他们对扩大农业面积、现代化设备和采用环境创新（如废物管理和节能解决方案）也有浓厚的兴趣。生产者对其活动对环境的影响以及对财政支助和技术培训的需要的认识有限。对研发项目的回顾表明，环境创新主要是在农业设备中以生物可降解材料代替塑料。然而，新兴技术尚未出现，而废物管理解决方案似乎更为可行。与专家的访谈和讨论进一步强调了该行业的结构性弱点，这些弱点既破坏了发展，也破坏了创新的倾向。研究结果表明，促进贻贝养殖的环境创新需要综合战略，将政策诱导机制（包括财政激励、培训、质量控制和提高生产标准）与持续的技术变革实验和对必须应用创新的特定社会环境背景的深刻理解结合起来。

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引用次数: 0

Development and validation of an RPA-CRISPR/Cas12a based platform for rapid and sensitive detection of Anguillid herpesvirus 1 基于RPA-CRISPR/Cas12a的安圭利德疱疹病毒1型快速灵敏检测平台的建立与验证

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-22 DOI: 10.1016/j.aquaculture.2026.743692

Bin Sun, Qiang Chen, Jun-Qing Ge

Anguillid herpesvirus 1 (AngHV), an enveloped double-stranded DNA virus, is the causative agent of “mucus sloughing and haemorrhagic septicaemia” disease of eels, leading to significant economic losses to the eel farming industry in China. Due to the absence of commercially effective drugs and vaccines, early and accurate diagnosis of AngHV is critical for disease prevention and management. However, existing diagnostic methods are often time-consuming or necessitate specialized equipment. In this study, we developed a CRISPR-based detection platform that enables rapid and sensitive detection of AngHV, utilizing either fluorescent or lateral flow assay readouts. This platform combines recombinase polymerase amplification (RPA) and the CRISPR/Cas12a system, in which nucleic acid targets are amplified by RPA reaction and subsequently detected by Cas12a-mediated cleavage, producing an observable signal under blue/UV light or via a lateral flow strip (LFS). The platform can accurately detect AngHV within 30 min at 40 °C, exhibited no cross-reactivity with other common aquatic viral pathogens, and possesses a limit of detection as low as 50 copies/μL of AngHV. Additionally, thirty clinical eel samples were evaluated using conventional PCR, quantitative PCR (qPCR), and the RPA-CRISPR/Cas12a platform. The positive detection rate of the developed platform was consistent with that of qPCR and more sensitive than that of conventional PCR. Overall, the developed RPA-CRISPR/Cas12a detection platform offers a rapid, sensitive, and reliable tool for on-site diagnosis of AngHV, thereby contributing to improved disease surveillance and control strategies in eel aquaculture.

鳗鲡疱疹病毒1 （AngHV）是一种包膜双链DNA病毒，是鳗鱼“粘液脱落和出血性败血症”病的病原体，给中国鳗鲡养殖业造成重大经济损失。由于缺乏商业上有效的药物和疫苗，早期和准确诊断AngHV对于疾病预防和管理至关重要。然而，现有的诊断方法往往耗时或需要专门的设备。在这项研究中，我们开发了一种基于crispr的检测平台，可以利用荧光或侧流分析读数快速灵敏地检测AngHV。该平台结合了重组酶聚合酶扩增（RPA）和CRISPR/Cas12a系统，通过RPA反应扩增核酸靶点，随后通过Cas12a介导的裂解检测，在蓝光/紫外光下或通过侧流条带（LFS）产生可观察的信号。该平台在40℃条件下可在30 min内准确检测出AngHV，与其他常见水生病毒病原体无交叉反应，检测限低至50拷贝/μL。此外，采用常规PCR、定量PCR （qPCR）和RPA-CRISPR/Cas12a平台对30份临床鳗鱼样本进行评估。该平台的阳性检出率与qPCR一致，比传统PCR更灵敏。总之，所建立的RPA-CRISPR/Cas12a检测平台为现场诊断AngHV提供了一种快速、灵敏、可靠的工具，有助于改善鳗鱼养殖疾病监测和控制策略。

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引用次数: 0

The phylogenetics and pathogen population dynamics of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in global shrimp populations 全球虾群中感染性皮下和造血坏死病毒（IHHNV）的系统发育和病原体种群动态

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-21 DOI: 10.1016/j.aquaculture.2026.743684

Emmanuel Ofori-Amponsah , Maria Andrade Martinez , Michael T. Meehan , Kelly Condon

Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) has posed a persistent threat to global shrimp aquaculture since its emergence in the 1980s, with transmission exacerbated by intensified farming and international trade. While previous studies have focused on regional outbreaks, the global evolutionary dynamics of IHHNV remain poorly understood. Here, we analyse 126 IHHNV capsid protein sequences (107 sequences extracted from GenBank and 19 newly collected sequences from northern Queensland, Australia) and 63 NS1 gene sequences (44 from GenBank and 19 from northern Queensland, Australia) collected from 12 countries between 1986 and 2022. Bayesian coalescent analyses estimate a mean nucleotide substitution rate of 4.5 × 10⁻⁴ (95% HPD 2.8 × 10⁻⁴, 6.4 × 10⁻⁴) substitutions/site/year for the capsid region and 1.9 × 10⁻⁴ (95% HPD 1.1 × 10⁻⁴, 2.8 × 10⁻⁴) for NS1, consistent with earlier region-specific results. The time to the most recent common ancestor was dated to the early 1980s (95% HPD: 1976–1986). Demographic reconstruction revealed periods of exponential growth in the 1990s and early 2000s, followed by a decline in the mid-2010s. Phylogenetic analysis revealed marked genetic diversity and strong geographic structuring, with some evidence of international viral exchange. Distinct regional SNP patterns mirrored the phylogenetic structure, supporting geographically constrained viral evolution and diversification. The high evolutionary rates and structured global distribution of IHHNV underscore the need for continued molecular surveillance and enhanced biosecurity to mitigate the risk of future outbreaks.

传染性皮下和造血坏死病毒（IHHNV）自20世纪80年代出现以来，一直对全球对虾养殖业构成持续威胁，集约化养殖和国际贸易加剧了传播。虽然以前的研究侧重于区域暴发，但对IHHNV的全球进化动态仍然知之甚少。在此，我们分析了1986年至2022年间从12个国家收集的126条IHHNV衣壳蛋白序列（107条序列来自GenBank， 19条新收集的序列来自澳大利亚昆士兰州北部）和63条NS1基因序列（44条来自GenBank， 19条来自澳大利亚昆士兰州北部）。贝叶斯聚结分析估计衣壳区的平均核苷酸取代率为4.5 × 10−4 (95% HPD为2.8 × 10−4,6.4 × 10−4)/位点/年，NS1区的平均核苷酸取代率为1.9 × 10−4 (95% HPD为1.1 × 10−4,2.8 × 10−4)，与先前的区域特异性结果一致。最近的共同祖先可以追溯到20世纪80年代初（95% HPD: 1976-1986）。人口重建显示，20世纪90年代和21世纪初出现了指数增长时期，随后在2010年代中期出现了下降。系统发育分析显示遗传多样性显著，地理结构强，有一定的国际病毒交换证据。不同的区域SNP模式反映了系统发育结构，支持地理上受限的病毒进化和多样化。IHHNV的高进化率和有组织的全球分布强调需要继续进行分子监测和加强生物安全，以减轻未来暴发的风险。

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引用次数: 0

AiTFAM modulates growth and thermotolerance of scallop Argopecten irradians irradians via regulating mitochondrial dynamics AiTFAM通过调节线粒体动力学调节扇贝的生长和耐热性

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-21 DOI: 10.1016/j.aquaculture.2026.743686

Xiaoxing Zhao , Jiaxi Chang , Junhao Zhang , Longfei Chu , Zhicheng Ruan , Chao Yu , Ancheng Liu , Jian Liu , Lisui Bao , Xiaoting Huang , Qiang Xing , Zhenmin Bao

Mitochondrial transcription factor A (TFAM) is a core regulator of mitochondrial DNA (mtDNA) replication and transcription, playing a vital role in cellular energy metabolism and physiological traits regulation. However, its function in marine bivalves remains poorly understood. In this study, a TFAM ortholog (AiTFAM) was identified in the bay scallop Argopecten irradians irradians via genome-wide homology-based screening. Structural and phylogenetic analyses revealed that AiTFAM, like its counterpart in the Yesso scallop, possesses three high-mobility group (HMG) domains—a distinctive feature that may support adaptation to dynamic intertidal environments. Spatiotemporal expression analysis demonstrated that AiTFAM transcript levels were positively correlated with mtDNA copy number throughout embryonic/larval development and across adult tissues, reflecting stage- and tissue-specific energy demands. Functional investigations using siRNA-mediated RNA interference revealed that AiTFAM knockdown led to a significant reduction in mtDNA copy number. In 24-h short-term experiments, this suppression impaired individual's thermotolerance, as indicated by a decreased Arrhenius break temperature (ABT) and a premature peak in the rate-amplitude product (RAP). In the 30-day long-term knockdown experiment, AiTFAM suppression led to growth retardation and significant defects in adductor muscle development, characterized by impaired hypertrophy and hyperplasia of muscle fibers. These findings demonstrate that AiTFAM modulates thermotolerance and growth in bay scallops by regulating mitochondrial dynamics, highlighting its potential as a molecular marker for breeding stress-resistant and fast-growing scallop varieties.

线粒体转录因子A （Mitochondrial transcription factor A， TFAM）是线粒体DNA （Mitochondrial DNA, mtDNA）复制和转录的核心调控因子，在细胞能量代谢和生理性状调控中起着至关重要的作用。然而，它在海洋双壳类动物中的作用仍然知之甚少。在本研究中，通过全基因组同源性筛选，在海湾扇贝Argopecten irradians irradians中鉴定了一个TFAM同源物（AiTFAM）。结构和系统发育分析表明，AiTFAM与Yesso扇贝一样，具有三个高迁移群（HMG）结构域，这一独特的特征可能支持对动态潮间带环境的适应。时空表达分析表明，AiTFAM转录水平与整个胚胎/幼虫发育和成体组织的mtDNA拷贝数呈正相关，反映了阶段和组织特异性的能量需求。使用sirna介导的RNA干扰进行功能研究显示，AiTFAM敲低导致mtDNA拷贝数显著减少。在24小时的短期实验中，这种抑制损害了个体的耐热性，表现为Arrhenius断裂温度（ABT）降低，速率-振幅乘积（RAP）提前达到峰值。在30天的长期敲低实验中，AiTFAM抑制导致生长迟缓，内收肌发育出现明显缺陷，表现为肌纤维肥厚增生受损。这些研究结果表明，AiTFAM通过调节线粒体动力学调节海湾扇贝的耐热性和生长，突出了其作为培育抗逆性和速生扇贝品种的分子标记的潜力。

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引用次数: 0

Genetic and non-genetic factors influencing reproductive traits of female Clarias magur in a line selected for improved harvest body weight 遗传和非遗传因素对改良收获体重的克拉丽亚斯母系生殖性状的影响

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-21 DOI: 10.1016/j.aquaculture.2026.743690

Kishor B. Gowda , Shrinivas Jahageerdar , Mujahidkhan Pathan , Jayasankar Jayaraman

The Indian catfish species Clarias magur is a potential candidate for aquaculture. ICAR-CIFE is implementing a genetic selection programme to harness its production potential by improving harvest body weight. This study estimated genetic parameters and quantified the effects of non-genetic factors on female reproductive traits in selectively bred individuals. Reproductive traits were recorded from 617 individuals across 174 families over three generations during spawning seasons from 2016 to 2021. The spawning age significantly affected all reproductive traits. In contrast, the spawning year, body weight at tagging (TagBW), and pond significantly affected pre-stripping weight (preSW), post-stripping weight (postSW), and spawning success (SS) (p < 0.05). Heritability estimates for preSW, postSW, total egg weight (TEW) and egg weight to body weight ratio (EBI) were 0.37 ± 0.10, 0.35 ± 0.09, 0.38 ± 0.11 and 0.22 ± 0.12, respectively. Genetic correlations between reproductive traits ranged from −0.41 to 0.98. Heritability estimates of reproductive traits suggest potential for genetic improvement. The genetic correlation between the preSW and TEW (0.33 ± 0.15) indicates a moderate association, suggesting that selecting magur for body weight may lead to a favourable correlated response in TEW. The overall SS was 37.60%, with 41.69% in males and 34.85% in females. SS exhibited moderate heritability on the logit (observed) scale (0.42 ± 0.01), indicating genetic variation in this binary trait. Overall, these results indicate that reproductive traits possess additive genetic variance, allowing for direct selection, and correlation with preSW suggests that preSW can be used as a selection criterion to improve reproductive performance in this species.

印度鲶鱼品种Clarias magur是一个潜在的水产养殖候选者。ICAR-CIFE正在实施一项遗传选择计划，通过改善收获体重来利用其生产潜力。本研究估计了遗传参数，量化了非遗传因素对选择性育种个体雌性生殖性状的影响。在2016年至2021年的产卵季节，记录了174个家庭三代617只个体的生殖特征。产卵年龄对各生殖性状影响显著。产卵年份、标记时体重（TagBW）和池塘对脱壳前体重（preSW）、脱壳后体重（postSW）和产卵成功率（SS）有显著影响（p < 0.05）。preSW、postSW、总蛋重（TEW）和蛋重体重比（EBI）的遗传力分别为0.37±0.10、0.35±0.09、0.38±0.11和0.22±0.12。生殖性状的遗传相关系数为- 0.41 ~ 0.98。生殖性状的遗传力估计表明遗传改良的潜力。preSW与TEW的遗传相关为（0.33±0.15），提示体重选择magur可能导致TEW的有利相关反应。总SS为37.60%，其中雄性41.69%，雌性34.85%。在logit （observed）标度上，SS表现出中等的遗传力（0.42±0.01），表明该二元性状存在遗传变异。总体而言，这些结果表明生殖性状具有可加性遗传变异，允许直接选择，并且与preSW的相关性表明preSW可以作为提高该物种生殖性能的选择标准。

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引用次数: 0

Targeting ACSL4: A potential strategy to alleviate deoxynivalenol-mediated intestinal ferroptosis, oxidative stress, and immunosuppression in Channa argus 靶向ACSL4：一种潜在的策略来缓解脱氧雪腐镰刀菌醇介导的肠铁中毒、氧化应激和免疫抑制

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743675

Yi-Ran Zhao , Tong-Hao Wang , Ze-Xin Zhao , Xiao-Tian Niu , Hong-Ming Lv , Yan-Bing Li , Yang Cao , Mu-Yang Li

In the present study, we evaluated the effects of deoxynivalenol (DON) on intestinal oxidative stress, immunity, ferroptosis, and endoplasmic reticulum stress in Channa argus. Furthermore, we investigated the mechanism underlying DON-induced cytotoxicity by inhibiting ACSL4 in macrophages. In vivo analysis demonstrated DON inhibits growth and even leading to increased mortality. DON induces intestinal ROS and iron ion accumulation, thereby causing endoplasmic reticulum dysfunction and triggering oxidative stress and ferroptosis. This process is accompanied by decreased levels of ferroptosis-inhibiting proteins (GPX4, FPN1, and FSP1), along with increased ACSL4 and ROS levels. Furthermore, DON-induced ROS accumulation elicits endoplasmic reticulum dysfunction and drives the progression of immunosuppression and inflammation. In vitro analysis demonstrated that inhibition of ACSL4 affects macrophage activity as well as ROS and ATP levels, representing a potential intervention strategy to restore immune cell function. This study demonstrates that targeting ACSL4 may serve as a potential strategy for alleviating DON-mediated intestinal injury in C. argus.

在本研究中，我们评估了脱氧雪腐镰刀鱼醇（DON）对鳢肠氧化应激、免疫、铁中毒和内质网应激的影响。此外，我们研究了don通过抑制巨噬细胞ACSL4诱导细胞毒性的机制。体内分析表明，DON抑制生长，甚至导致死亡率增加。DON诱导肠道ROS和铁离子积累，引起内质网功能障碍，引发氧化应激和铁下垂。这一过程伴随着凋亡抑制蛋白（GPX4、FPN1和FSP1）水平的降低，以及ACSL4和ROS水平的升高。此外，don诱导的ROS积累引起内质网功能障碍，并推动免疫抑制和炎症的进展。体外分析表明，抑制ACSL4影响巨噬细胞活性以及ROS和ATP水平，代表了恢复免疫细胞功能的潜在干预策略。该研究表明，靶向ACSL4可能是缓解don介导的大肠杆菌肠道损伤的潜在策略。

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引用次数: 0

Single nuclei transcriptomics reveals programming of metabolic functions by embryonic temperature in the developing Atlantic salmon liver 单核转录组学揭示了大西洋鲑鱼肝脏发育过程中胚胎温度对代谢功能的影响

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743685

Carl I.J. Milton , Richard S. Taylor , Jianxuan Sun , Carlo C. Lazado , Nicholas M. Wade , Erik Burgerhout , Daniel J. Macqueen

Environmental temperature pervasively affects the physiology of poikilothermic fishes. Embryogenesis is a sensitive period of development, where altering temperature can promote plasticity in phenotypes that persist deeper into the life cycle, with potential implications for aquaculture. However, the mechanisms by which embryonic temperature shapes phenotypic plasticity remain poorly understood. Here, we investigate the impact of embryonic temperature regime on the transcriptome of 15,688 liver cells from farmed Atlantic salmon fry. Three fish groups were grown at 4, 6 or 8 °C solely from fertilisation to the eyed-stage of embryogenesis, then reared at the same temperature until sampling, one-month post start-feeding (three months post treatment). Single nuclei transcriptomics revealed hepatocyte, cholangiocyte, mesenchymal, endothelial and immune cell populations. Seven hepatocyte subpopulations were identified with distinct expression and functional characteristics. We identified strong impacts of embryonic temperature on the transcriptome of hepatocytes, implicating diverse metabolic functions, pathways and regulatory genes. This included changes in mevalonate and steroid biosynthesis pathways, as well as the growth hormone-insulin-like growth factor axis, consistent with alterations in cholesterol/lipid metabolism and the endocrine regulation of growth. We provide evidence for increased antiviral gene expression in the 4 °C group, alongside significant changes in the expression of epigenetic and stress regulation genes across temperature groups. Collectively, our results indicate that altering temperature for a short window of Atlantic salmon embryogenesis programs the development of hepatic, metabolic and immune functions deeper into the aquaculture production cycle.

环境温度普遍影响着变温鱼类的生理机能。胚胎发生是发育的一个敏感时期，温度的变化可以促进表型的可塑性，这种可塑性会持续到生命周期的更深处，对水产养殖有潜在的影响。然而，胚胎温度塑造表型可塑性的机制仍然知之甚少。在这里，我们研究了胚胎温度对养殖大西洋鲑鱼鱼苗15688个肝细胞转录组的影响。三组鱼从受精到胚胎眼期分别在4、6或8°C的温度下生长，然后在相同的温度下饲养直到取样，即开始喂食后一个月（处理后三个月）。单核转录组学揭示了肝细胞、胆管细胞、间充质细胞、内皮细胞和免疫细胞群。七个肝细胞亚群被鉴定出具有不同的表达和功能特征。我们发现了胚胎温度对肝细胞转录组的强烈影响，涉及多种代谢功能、途径和调控基因。这包括甲羟戊酸盐和类固醇生物合成途径的变化，以及生长激素-胰岛素样生长因子轴的变化，与胆固醇/脂质代谢和生长内分泌调节的变化一致。我们提供的证据表明，在4°C组中抗病毒基因表达增加，同时表观遗传和应激调节基因的表达在温度组中也发生了显著变化。总的来说，我们的研究结果表明，在大西洋鲑鱼胚胎发生的短时间内改变温度可以使肝脏、代谢和免疫功能的发育深入到水产养殖生产周期。

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引用次数: 0

Myo-inositol-mediated efferocytosis promotes lysosomal function and ROS clearance related to the pentose phosphate pathway in fish muscle 肌醇介导的efferocytosis促进了鱼类肌肉中与戊糖磷酸途径相关的溶酶体功能和ROS清除

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743681

Meiqi Wang , Lin Feng , Pei Wu , Yang Liu , Hongyun Zhang , Yaobin Ma , Hongmei Ren , Xiaowan Jin , Xiaoqiu Zhou , Weidan Jiang

Skeletal muscle constitutes the main consumable part of fish, and its quality and physiological integrity are critical to the sustainable advancement of aquaculture. This investigation focused on the consequences of dietary myo-inositol (MI) deficiency on muscle homeostasis in grass carp (Ctenopharyngodon idella) using six experimental diets with varying amounts of MI: 35 (basal diet, deficient group), 98, 195, 292, 389, and 487 mg/kg. Fish were fed for 8 weeks. Transcriptome analysis of grass carp muscle tissue was first performed, revealing that efferocytosis and the pentose phosphate pathway (PPP) were among the primary metabolic pathways affected by MI intervention. Further investigation demonstrated that MI administration within the range of 195–487 mg/kg effectively activated the PPP, as indicated by significantly increased activities of two key enzymes in its oxidative branch-glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH). This enzymatic activation led to enhanced NADPH production. The consequent rise in NADPH levels resulted in decreased intracellular ratios of NADP⁺/NADPH and GSH/GSSG, reflecting a strengthened reductive cellular environment and thereby improving the capacity to scavenge reactive oxygen species (ROS). Furthermore, MI reduces cell death in muscle tissue and enhance macrophage-mediated efferocytosis. Concurrently, MI inhibits the polarization of macrophages towards pro-inflammatory phenotypes by enhancing lysosomal function. Therefore, the inclusion of appropriate levels of MI (195–389 mg/kg) in the diet is beneficial for maintaining muscle tissue homeostasis in grass carp.

骨骼肌是鱼类的主要食用部分，其质量和生理完整性对水产养殖的可持续发展至关重要。本研究主要研究了草鱼（Ctenopharyngodon idella）饲料中肌醇（MI）缺乏对肌肉稳态的影响，采用6种不同MI含量的试验饲料：35（基础饲料，缺乏组）、98、195、292、389和487 mg/kg。饲喂8周。首先对草鱼肌肉组织进行转录组分析，发现心肌梗死干预影响的主要代谢途径包括胞吐和戊糖磷酸途径（PPP）。进一步的研究表明，在195-487 mg/kg范围内的MI可以有效激活PPP，其氧化分支中两个关键酶-葡萄糖-6-磷酸脱氢酶（G6PDH）和6-磷酸葡萄糖酸脱氢酶（6PGDH）的活性显著增加。这种酶激活导致NADPH产生增强。NADPH水平随之升高，导致细胞内NADP+/NADPH和GSH/GSSG比值降低，反映了还原性增强的细胞环境，从而提高了清除活性氧（ROS）的能力。此外，心肌梗死减少肌肉组织细胞死亡，增强巨噬细胞介导的efferocytosis。同时，MI通过增强溶酶体功能抑制巨噬细胞向促炎表型的极化。因此，在饲料中添加适当水平的心肌蛋白（195 ~ 389 mg/kg）有利于草鱼维持肌肉组织稳态。

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引用次数: 0

Projecting the future of aquaculture in Egypt under climate and socio-economic scenarios 在气候和社会经济情景下预测埃及水产养殖的未来

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743677

Muhammed A. Oyinlola , Hussam Shaheen , Nada Habashi , Sameh Attia , Lydia C.L. Teh , Ahmed Nasr-Allah , William W.L. Cheung

Aquaculture is essential to Egypt's food and nutrition security, contributing over 1.5 million tonnes of fish annually, approximately 80% of the country's total fish production. However, the sector faces increasing risks from climate change and socio-economic transformations such as population growth, food demand, land-use dynamics, and governance shifts. Here, we adapt the GOMAP model for land-based systems to project aquaculture production potential (APP) under three Shared Socio-economic Pathways (SSP1–2.6, SSP2–4.5, SSP5–8.5) across five major governorates: Behera, Damietta, Kafr El-Sheikh, Ismailia and Port Said. Our analysis integrates species distribution models for key farmed fish species in Egypt, machine learning-based projections of pond water temperatures and the dietary demands of farmed species. Results indicate that under SSP1–2.6, most regions sustain or improve their APP through the 21st century, with governorates like Ismailia and Port Said maintaining 100% potential relative to the 2020s. In SSP2–4.5, however, APP becomes increasingly uneven; Behera and Kafr El-Sheikh decline by up to 79% and 74% respectively, by the 2090s, while Port Said and Ismailia retain higher resilience. Under SSP5–8.5, APP declines are most severe and widespread, especially for tilapia, with production potential falling below 50% in multiple regions by the end of the 21st century. Mullets and catfish show greater climate resilience across all scenarios. These findings highlight the urgent need for targeted adaptation strategies, including selective breeding, shading and aeration systems, and spatial reallocation to climatically stable regions. This modelling framework offers a valuable decision-support tool for ensuring sustainable and climate-resilient aquaculture development in Egypt.

水产养殖对埃及的粮食和营养安全至关重要，每年提供150多万吨鱼类，约占该国鱼类总产量的80%。然而，该部门面临着气候变化和社会经济转型（如人口增长、粮食需求、土地利用动态和治理转变）带来的越来越大的风险。在这里，我们将GOMAP模型应用于陆基系统，在Behera、Damietta、Kafr El-Sheikh、Ismailia和Port Said五个主要省份的三个共享社会经济路径（SSP1-2.6、SSP2-4.5、SSP5-8.5）下预测水产养殖生产潜力（APP）。我们的分析整合了埃及主要养殖鱼类的物种分布模型、基于机器学习的池塘水温预测和养殖物种的饮食需求。结果表明，在SSP1-2.6下，大多数地区在21世纪维持或改善其APP，其中Ismailia和Port Said等省份相对于21世纪20年代保持100%的潜力。然而，在SSP2-4.5中，APP变得越来越不均衡；到2090年代，Behera和Kafr El-Sheikh分别下降79%和74%，而Port Said和Ismailia则保持较高的弹性。根据SSP5-8.5， APP下降最为严重和广泛，尤其是罗非鱼，到21世纪末，多个地区的生产潜力将下降到50%以下。鲻鱼和鲶鱼在所有情况下都表现出更强的气候适应能力。这些发现强调了迫切需要有针对性的适应策略，包括选择性育种，遮阳和通风系统，以及向气候稳定地区的空间重新分配。这一建模框架为确保埃及水产养殖的可持续和气候适应型发展提供了宝贵的决策支持工具。

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引用次数: 0

Enhancing robustness of Pacific oyster (Crassostrea gigas) through breeding strategy based on larval phototaxis 基于幼虫趋光性的繁殖策略提高太平洋牡蛎（长牡蛎）的健壮性

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743688

Deqi Sun , Ben Yang , Chao Guo , Weiying Sun , Qi Li , Shikai Liu

The Pacific oyster (Crassostrea gigas) aquaculture industry faces significant challenges from recurrent summer mass mortality events attributed to synergistic environmental and biological stressors. To address this issue, an innovative breeding strategy was developed by engineering a phototactic behavior-based screening device to select oyster larvae with differential robustness. Using this custom-designed sorting apparatus, oyster larvae were categorized into distinct cohorts with variable robustness, anticipating that enhanced locomotor performance and response capacity after perceiving light might correlate with superior robustness throughout the life cycle. A comprehensive multi-stage field evaluation suggested significant performance superiority of strong robustness cohort over other cohorts. The strong robustness cohort demonstrated superior larval growth rate and survival rate. Remarkably, these advantages persisted through juvenile and adult stages, with enhanced thermal tolerance, improved resistance to Vibrio infection, and accelerated growth trajectories in both spat and adult stages. These findings underscore the potential of selective breeding through larval locomotor performance and response capacity after perceiving light based on phototaxis selection to enhance the robustness of C. gigas, toward developing environment resilient strains to advance the sustainability and productivity of oyster aquaculture.

太平洋牡蛎（长牡蛎）养殖业面临着由协同环境和生物应激因素引起的反复夏季大量死亡事件的重大挑战。为了解决这一问题，研究人员设计了一种基于光致行为的筛选装置，以选择具有不同稳健性的牡蛎幼虫，从而开发了一种创新的繁殖策略。使用这种定制设计的分类装置，牡蛎幼虫被分为不同的鲁棒性群体，预计感知光后增强的运动性能和反应能力可能与整个生命周期的优越鲁棒性相关。综合多阶段现场评价表明，强稳健性队列优于其他队列。强稳健性队列显示出优越的幼虫生长速度和存活率。值得注意的是，这些优势在幼鱼和成虫阶段持续存在，增强了热耐受性，提高了对弧菌感染的抵抗力，并加速了贝虫和成虫阶段的生长轨迹。这些发现强调了在趋光性选择的基础上，通过幼虫的运动性能和感知光后的反应能力进行选择性育种，以增强C. gigas的稳健性，从而开发具有环境适应性的菌株，从而提高牡蛎养殖的可持续性和生产力。

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引用次数: 0