Aquaculture最新文献_第3页

The contribution of host transfer in the infection dynamics of Caligus elongatus and salmon lice (Lepeophtheirus salmonis) in a salmon farming network 在鲑鱼养殖网络中，寄主转移对长钩鲑和鲑鱼虱感染动态的贡献

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743683

Gunnvør á Norði , Birgitta Andreasen , Kirstin Eliasen , Tróndur J. Kragesteen , Signar P. Dam , Lars Are Hamre

The sea lice Lepeophtheirus salmonis and Caligus elongatus have direct lifecycles, with infective copepodids attaching to hosts and developing into adults. However, increased delousing and fish handling have raised concern about whether preadult or adult lice stages that detach during crowding might reattach to other fish. C. elongatus have a significant reservoir of wild hosts, and little is known to which extent salmon in farms are infected directly by copepodids or by adults transferring from other hosts. In the Faroe Islands, third-party sea lice monitoring ensures consistent data collection across farms. The data is stored in a database including additional farm specific information. When fish are transferred to sea, attached copepodids require a temperature-dependent period to develop into pre-adult and adult stages. If these are found prematurely on the fish, they have likely attached as mobiles. Few L. salmonis were found prematurely, revealing that host transfer of preadult and adult L. salmonis between salmon cages was highly unusual with little impact on the overall epidemiology of salmon lice on the farmed fish. In contrast, transmission of adult C. elongatus was common, accounting for ∼44% of the total infections. C. elongatus abundances on farmed salmon were seasonal and new infections were dominated by adult host transfer in July when abundances started to increase. These contrasting transmission patterns highlight the need for species-specific management strategies and further research into the ecology and transmission dynamics of C. elongatus, whose epidemiological behavior and impact remain less understood despite its significant contribution to overall infection pressure.

沙门Lepeophtheirus salmonis和Caligus elongatus的海虱有直接的生命周期，感染的桡足类附着在宿主上并发育成成虫。然而，越来越多的除虫和对鱼的处理引起了人们的关注，即在拥挤期间分离的成虫或成虫阶段是否会重新附着在其他鱼身上。C. elongatus有大量的野生宿主，很少有人知道养殖场的鲑鱼在多大程度上直接被桡足类感染或被从其他宿主转移过来的成虫感染。在法罗群岛，第三方海虱监测确保了整个养殖场的一致数据收集。数据存储在数据库中，包括其他特定于农场的信息。当鱼被转移到海洋中时，附着的桡足类动物需要一段温度依赖期来发育为成虫前期和成虫阶段。如果过早地在鱼身上发现这些，它们很可能会作为手机附着在鱼身上。很少过早发现沙门氏菌，这表明在鲑鱼网箱之间，未成虫和成虫沙门氏菌的宿主转移非常罕见，对养殖鱼类鲑鱼虱的总体流行病学影响不大。相比之下，成年长形c虫的传播很常见，占总感染的44%。养殖三文鱼上的长形弧菌丰度具有季节性，新感染以7月开始丰度增加的成虫转移为主。这些截然不同的传播模式凸显了物种特异性管理策略的必要性，以及对长形卷虫生态学和传播动力学的进一步研究的必要性。尽管长形卷虫对总体感染压力有重要贡献，但其流行病学行为和影响仍鲜为人知。

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引用次数: 0

Corrigendum to “Immersion-based infection model of nervous necrosis virus in Oryzias pectoralis.” [Volume 614, 15 March 2026, 743572] “胸大角羚神经坏死病毒浸没感染模型”的勘误。[第614卷，2026年3月15日，743572]

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743640

Runqing Huang, Lin Liu, Siqi Qiu, Kun Ye, Zhuoyan Yin, Xiaoqing Zeng, Xinyue Chen, Xuechong Yan, Jiangyong Wang

引用次数: 0

The impact of blue economy on sustainable development: An empirical study of BRICS nations 蓝色经济对可持续发展的影响：基于金砖国家的实证研究

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743678

Rajalaxmi Mohanty, Sanjaya Kumar Lenka

The Blue Economy represents a transformative paradigm for harnessing oceanic resources to promote equitable and sustainable development. This study empirically analyses the impact of the Blue Economy on sustainable development in the BRICS nations over the period 2006–2024. Using a novel sustainability index constructed through Principal Component Analysis (PCA), this study integrates multidimensional indicators aligned with SDGs 1, 2, 8, 9, and 14. Key Blue Economy variables—total fisheries production, aquaculture, agriculture-forestry-fishing, marine protected areas, and ocean renewable energy inventions are analysed using a suite of robust panel econometric techniques, including Fixed Effects, Random Effects, Panel-Corrected Standard Errors (PCSEs), and Generalised Method of Moments (GMM). The findings reveal a significant positive association between sustainable development and variables such as aquaculture production, marine conservation, and ocean-based innovation, whereas trade openness and inflation exhibit a negative influence. These results underscore the strategic importance of ocean-centric policies and marine resource management in fostering ecological and economic synergies. This study contributes to the discourse on sustainability transitions in emerging economies and offers actionable insights for policymakers aiming to align national growth with SDG objectives.

蓝色经济是利用海洋资源促进公平和可持续发展的变革性范例。本研究实证分析了2006-2024年蓝色经济对金砖国家可持续发展的影响。本研究利用主成分分析（PCA）构建了一个新的可持续发展指数，整合了与可持续发展目标1、2、8、9和14相一致的多维指标。关键的蓝色经济变量——渔业生产总量、水产养殖、农林渔业、海洋保护区和海洋可再生能源发明——使用一套强大的面板计量经济学技术进行分析，包括固定效应、随机效应、面板校正标准误差（PCSEs）和广义矩量法（GMM）。研究结果表明，可持续发展与水产养殖生产、海洋保护和海洋创新等变量之间存在显著的正相关关系，而贸易开放和通货膨胀则表现出负面影响。这些结果强调了以海洋为中心的政策和海洋资源管理在促进生态和经济协同作用方面的战略重要性。本研究有助于对新兴经济体可持续转型的讨论，并为旨在使国家增长与可持续发展目标保持一致的政策制定者提供可操作的见解。

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引用次数: 0

Metabolic regulation of hypoxia-tolerant Grass Carp (Ctenopharyngodon idellus) under hypoxia stress 缺氧胁迫下耐缺氧草鱼的代谢调控

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-20 DOI: 10.1016/j.aquaculture.2026.743662

Zhongyu Zhang , Jingya Li , Xiaohui Li, Cui Feng, Nannan Feng, Hongwei Liang

The economic value and biological significance of grass carp (Ctenopharyngodon idellus) make it important to explore the potential molecular mechanisms of the species' response to hypoxia. In this study, metabolic characteristics of intestine, liver, muscle, brain, gill, heart and plasma compartments of grass carp in three groups: normoxic group (GC), hypoxia-tolerant group (GN) under hypoxic stress conditions, hypoxia-intolerant group (GB), were systematically investigated by using LC-MS/MS techniques. Comparative analysis of differential metabolites across the GC, GB, and GN groups identified 109, 123, 103, 103, 36, 67, 73, and 56 hypoxia tolerance-associated metabolites in seven tissues (intestine, liver, muscle, brain, gill, heart, and plasma), respectively. These metabolites were linked to 27 significant metabolic pathways and predominantly involved in the regulation of lipid, carbohydrate, and amino acid metabolic pathways. Further analysis revealed that hypoxia-tolerant grass carp elevate glycine and other amino acids in the liver and muscle, regulating cellular osmosis to adapt to hypoxia. Additionally, these fish reduced brain and gill pyruvate levels, increased fumarate synthesis, and utilized lactate transport to maintain energy balance under hypoxia. This metabolic adaptation activated systemic lipid metabolism, providing an effective response to hypoxic stress. Moreover, the hypoxia-tolerant grass carps preferentially utilized fatty acids from muscle and gills as an energy source, enhancing the oxidation of fatty acids, thereby reducing the synthesis and accumulation of triacylglycerol. In summary, this study elucidates the metabolic regulatory mechanisms underlying hypoxia adaptation in grass carp at the metabolomic level. It identifies key biomarkers of grass carp such as glycine, pyruvate, fumarate, and triacylglycerol, providing a foundational understanding of the physiological adjustments under hypoxic stress.

草鱼（Ctenopharyngodon idellus）的经济价值和生物学意义使得探索该物种对缺氧反应的潜在分子机制具有重要意义。本研究采用LC-MS/MS技术，系统研究了常氧组（GC）、耐缺氧组（GN）和缺氧不耐受组（GB） 3组草鱼的肠道、肝脏、肌肉、脑、鳃、心脏和血浆室的代谢特性。对比分析了GC组、GB组和GN组的差异代谢物，分别在7个组织（肠、肝、肌肉、脑、鳃、心脏和血浆）中鉴定出109、123、103、103、36、67、73和56种低氧耐受性相关代谢物。这些代谢物与27个重要的代谢途径有关，主要参与脂质、碳水化合物和氨基酸代谢途径的调节。进一步分析表明，耐缺氧草鱼提高肝脏和肌肉中的甘氨酸和其他氨基酸，调节细胞渗透以适应缺氧。此外，这些鱼降低了脑和鳃的丙酮酸水平，增加了富马酸的合成，并利用乳酸运输来维持缺氧下的能量平衡。这种代谢适应激活了全身脂质代谢，提供了对缺氧应激的有效反应。此外，耐缺氧草鱼优先利用来自肌肉和鳃的脂肪酸作为能量来源，促进脂肪酸的氧化，从而减少甘油三酯的合成和积累。综上所述，本研究在代谢组学水平上阐明了草鱼缺氧适应的代谢调控机制。鉴定草鱼的甘氨酸、丙酮酸、富马酸和甘油三酯等关键生物标志物，为草鱼在缺氧胁迫下的生理调节提供基础认识。

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引用次数: 0

Population identification in northern snakehead (Channa argus) through machine learning integration of informative SNPs from whole-genome sequencing and 50 K SNP Array 利用机器学习整合全基因组测序和50 K SNP阵列的信息性SNP对北方黑鱼（Channa argus）种群进行鉴定

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-19 DOI: 10.1016/j.aquaculture.2026.743659

Jin Zhang , Tongxin Cui , Ziwen Yang , Jiajiu Liu , Jiafeng Liu , Mi Ou , Qing Luo , Shuzhan Fei , Xinping Zhu , Kunci Chen , Jian Zhao , Haiyang Liu

Northern snakehead (Channa argus) in China harbors rich genetic resources and pronounced phenotypic diversity, underpinning its high commercial and economic value in aquaculture. However, the rapid expansion of intensive farming has rendered traditional morphology-based identification and conventional molecular markers inadequate for accurate, efficient, and reproducible population discrimination, thereby limiting the effective utilization and conservation of genetic resources. In this study, we developed a robust population identification framework for Northern snakehead (C. argus) by integrating whole-genome sequencing (WGS) and SNP array data with machine learning approaches. We systematically evaluated three trait SNP selection strategies (Delta, F_ST, and I_n) and their combinations across multiple machine learning models (ANN, KNN, NB, RF, SVM, and XGBoost) under different reference population sizes and marker densities. Results showed that within WGS-based data, the DFI detection strategy outperformed or equaled other single methods. Among machine learning models, ANN, SVM, and RF demonstrated superior classification performance compared with KNN, NB, and XGBoost. Notably, the integrated ASR (ANN, SVM, and RF) combined with DFI consistently outperformed any single model, achieving an accuracy of 0.95 when the number of trait loci reached approximately 1500. In contrast, low-density SNP array data achieved the same accuracy (0.95) with only 200 markers, regardless of whether loci were DFI-selected or randomly sampled, demonstrating clear advantages over WGS-derived SNP panels in terms of efficiency and cost-effectiveness. These results underscore the importance of rational marker selection for small-scale datasets and demonstrate that SNP array data are sufficient to support high-precision population identification in large-scale or practical aquaculture applications. Overall, this study provides an effective and economical strategy for population certification and genetic resource management of Northern snakehead (C. argus), with broad applicability to other aquaculture species.

中国北方黑鱼（Channa argus）遗传资源丰富，表型多样性显著，具有很高的养殖商业和经济价值。然而，随着集约化农业的迅速发展，传统的基于形态的鉴定和传统的分子标记已不足以实现准确、高效和可复制的群体鉴别，从而限制了遗传资源的有效利用和保护。在这项研究中，我们将全基因组测序（WGS）和SNP阵列数据与机器学习方法相结合，开发了一个强大的北方蛇头（C. argus）种群识别框架。在不同参考种群规模和标记密度下，我们系统地评估了三种性状SNP选择策略（Delta、FST和In）及其在多个机器学习模型（ANN、KNN、NB、RF、SVM和XGBoost）上的组合。结果表明，在基于wgs的数据中，DFI检测策略优于或等于其他单一方法。在机器学习模型中，与KNN、NB和XGBoost相比，ANN、SVM和RF表现出更好的分类性能。值得注意的是，综合ASR （ANN， SVM和RF）与DFI相结合，始终优于任何单一模型，当性状位点数量达到约1500时，准确率达到0.95。相比之下，低密度SNP阵列数据仅使用200个标记获得相同的准确性（0.95），无论位点是dfi选择还是随机抽样，在效率和成本效益方面都比wgs衍生的SNP面板具有明显优势。这些结果强调了对小规模数据集进行合理标记选择的重要性，并表明SNP阵列数据足以支持大规模或实际水产养殖应用中的高精度种群识别。本研究为北方黑鱼种群认证和遗传资源管理提供了一种经济有效的策略，对其他水产养殖品种具有广泛的适用性。

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引用次数: 0

Multi-omics analysis investigates the effects of replacing fishmeal with intestinal mucosal protein on growth performance and intestinal health in hybrid sturgeon 多组学分析研究了肠黏膜蛋白替代鱼粉对杂交鲟生长性能和肠道健康的影响

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-19 DOI: 10.1016/j.aquaculture.2026.743679

Yuanfa He , Jun Wen , Hang Chen , Guangjun Lv , Yongjun Chen , Shimei Lin , Haiping Liu , Zhijian Wang , Hailong Ge

This study evaluated hydrolyzed porcine intestinal mucosa protein (IMP) as a sustainable fishmeal alternative for hybrid sturgeon (Acipenser baerii ♀ × Acipenser schrenckii ♂). Six isonitrogenous and isolipidic diets were formulated with IMP replacing 0% (IMP0), 5% (IMP5), 10% (IMP10), 20% (IMP20), 40% (IMP40), and 60% (IMP60) of fishmeal. Juvenile hybrid sturgeons (initial weight 14.00 ± 0.30 g) were fed the diets in triplicate for 60 days. Results showed fish meal replacement level significantly influenced growth performance (weight gain rate, feed conversion ratio, feeding rate, and protein efficiency ratio; P < 0.001), with linear and quadratic relationships (P < 0.001). When replacement exceeded 20%, weight gain rate showed a significant decline (P < 0.05). H&E staining and microstructure showed that the IMP10 group maintained intact plicae and regularly arranged villi; intestinal structural damage emerged in the IMP20 group, while the IMP60 group exhibited intestinal villus breakage and shedding, thinning of the muscular layer, and increased cell apoptosis. Intestinal permeability markers (diamine oxidase, D-lactic acid, and lipopolysaccharide) increased significantly at the 20% replacement level (P < 0.05). The IMP10 group had higher microbial abundance and diversity compared to the IMP0 group, while the IMP60 group showed significantly lower diversity (P < 0.05) and abundance of the beneficial bacterium Cetobacterium (P < 0.05). Transcriptome analysis indicated that the IMP10 and IMP0 comparison groups were significantly enriched in pathways related to protein digestion and absorption (Q < 0.001) and pancreatic secretion (Q < 0.001). Metabolome results showed significant upregulation of long-chain fatty acids and inosine (P < 0.05). In contrast, the IMP60 and IMP0 comparison groups exhibited significant enrichment of stress-related pathways, including staphylococcus aureus infection and inflammatory bowel disease, along with activation of the pentose phosphate pathway (P < 0.05). Mantel test analysis revealed significant correlations of weight gain rate, trypsin, and lipopolysaccharide with Plesiomonas, lcat gene, and ketoleucine (P < 0.05). In conclusion, IMP can effectively replace 10% of fishmeal in hybrid sturgeon diets without adverse effects. High replacement levels, however, tend to induce intestinal structural damage, microbial alterations, and metabolic disturbances, ultimately affecting growth performance and intestinal health. This study provides a scientific basis for formulating sustainable feeds for hybrid sturgeon aquaculture and offers multi-omics insights into the functional mechanisms of alternative protein sources.

本研究评价了水解猪肠黏膜蛋白（IMP）作为杂交鲟（baerii♀× schrenckii♂）的可持续鱼粉替代品。以IMP代替0% （IMP0）、5% （IMP5）、10% （IMP10）、20% （IMP20）、40% （IMP40）和60% （IMP60）的鱼粉配制6种等氮等脂饲料。杂交中华鲟幼鱼（初始体重14.00±0.30 g）分3次投喂60 d。结果表明：鱼粉替代水平对生长性能（增重率、饲料系数、摄食率和蛋白质效率）有显著影响（P < 0.001），且呈线性和二次关系（P < 0.001）。替代量超过20%时，增重率显著下降（P < 0.05）。H&；E染色和微观结构显示，IMP10组保持完整的皱襞和排列规则的绒毛；IMP20组出现肠结构损伤，IMP60组肠绒毛断裂脱落，肌层变薄，细胞凋亡增加。在20%替代水平下，肠道通透性指标（二胺氧化酶、d -乳酸和脂多糖）显著升高（P < 0.05）。IMP10组微生物丰度和多样性高于IMP0组，而IMP60组有益菌群的多样性（P < 0.05）和丰度显著低于IMP0组（P < 0.05）。转录组分析显示，IMP10和IMP0对照组在蛋白质消化吸收（Q < 0.001）和胰腺分泌（Q < 0.001）相关通路中显著富集。代谢组学结果显示，长链脂肪酸和肌苷显著上调（P < 0.05）。相比之下，IMP60和IMP0对照组表现出显著的应激相关途径的富集，包括金黄色葡萄球菌感染和炎症性肠病，以及戊糖磷酸途径的激活（P < 0.05）。Mantel试验分析显示，增重率、胰蛋白酶和脂多糖与Plesiomonas、lcat基因和酮氨酸呈显著相关（P < 0.05）。综上所述，IMP可有效替代杂交鲟饲料中10%的鱼粉，且无不良反应。然而，高替代水平往往会引起肠道结构损伤、微生物改变和代谢紊乱，最终影响生长性能和肠道健康。本研究为杂交鲟水产养殖可持续饲料的制定提供了科学依据，并为替代蛋白质来源的功能机制提供了多组学视角。

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引用次数: 0

Characteristics of microbial communities in water, sediment, and fish intestines: A comparison between lotus-fish co-culture and intensive pond culture systems and their potential ecological impacts 水、沉积物和鱼肠中微生物群落的特征：莲花鱼共养和集约化池塘养殖系统的比较及其潜在的生态影响

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-19 DOI: 10.1016/j.aquaculture.2026.743673

Shengnan Li, Xinyu Xie, Zhe Wang, Xuting Ye, Lu Yang, Zhongyuan Shen, Kaikun Luo, Qianhong Gu

Microbial communities are vital for aquaculture ecosystem health. This study evaluated the effects of two distinct aquaculture modes, lotus-fish co-culture (LFC) and conventional intensive pond culture (IPC), on microbial diversity and composition in water, sediment, and fish gut. Using metagenomic sequencing combined with microbial source tracking and partial least squares path modeling (PLS-PM), we systematically examined microbial variations across different aquaculture modes and times, and identified the underlying driving factors. The results showed that 60% of microbial species were shared across fish gut, sediment and water habitats. While both mode and time influenced microbial communities, aquaculture mode was the dominant factor, particularly in the fish gut. Compared to IPC, LFC significantly increased fish gut microbial alpha diversity (p < 0.05) and reduced the relative abundance of several taxa containing potential pathogens, such as Aeromonas, Romboutsia, Clostridium, as well as viruses and Microsporidia. Source tracking analysis indicated that 31.0% of gut microbiota in LFC originated from sediment, significantly higher than the 11.6% observed in IPC. PLS-PM revealed that aquaculture mode indirectly influenced gut microbiota by altering water quality (e.g., TN, DO, COD,) and sediment properties (e.g., TN, TP, NH₄⁺-N), thereby reshaping environmental microbial communities, while also exerting direct effects potentially via host behavior and diet. These findings demonstrate that LFC enhances fish gut microbial diversity and suppresses potential pathogens through a structured “environment–microbiota–host” pathway, supporting the optimization of aquaculture modes for sustainable fish farming.

微生物群落对水产养殖生态系统的健康至关重要。本研究评价了荷鱼共养（LFC）和常规集约化池塘养殖（IPC）两种不同的养殖模式对水体、沉积物和鱼肠中微生物多样性和组成的影响。采用宏基因组测序、微生物源跟踪和偏最小二乘路径建模（PLS-PM）技术，系统分析了不同养殖模式和时间的微生物变异，并确定了潜在的驱动因素。结果表明，60%的微生物物种在鱼肠、沉积物和水生境中共享。虽然养殖方式和养殖时间都对微生物群落有影响，但养殖方式是主要因素，尤其是在鱼类肠道中。与IPC相比，LFC显著增加了鱼类肠道微生物α多样性（p < 0.05），并降低了含有潜在病原体的几个分类群的相对丰度，如气单胞菌、Romboutsia、Clostridium以及病毒和微孢子虫。来源跟踪分析表明，LFC中31.0%的肠道微生物群来自沉积物，显著高于IPC中11.6%的肠道微生物群。PLS-PM揭示了养殖模式通过改变水质（如TN、DO、COD）和沉积物性质（如TN、TP、NH4+-N）间接影响肠道微生物群，从而重塑环境微生物群落，同时也可能通过宿主行为和饮食产生直接影响。这些结果表明，LFC通过结构化的“环境-微生物-宿主”途径增强鱼类肠道微生物多样性，抑制潜在病原体，支持水产养殖模式的优化，实现鱼类可持续养殖。

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引用次数: 0

Genome-wide association study of resistance to infectious spleen and kidney necrosis virus in mandarin fish (Siniperca chuatsi) 鳜鱼抗传染性脾肾坏死病毒的全基因组关联研究

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-19 DOI: 10.1016/j.aquaculture.2026.743676

Yuqin Su , Shiyu Qu , Yang Liu , Jinmeng Teng , Junyan Zeng , Shuang Liu , Shuisheng Li , Songlin Chen

Mandarin fish (Siniperca chuatsi) is one of China's most economically important aquatic species, yet its farming is severely threatened by the infectious spleen and kidney necrosis virus (ISKNV). Enhancing disease resistance via genetic improvement is critical, but the genetic basis of ISKNV resistance in this species remains poorly understood. Here, we performed a genome-wide association study (GWAS) with 511 susceptible and 223 resistant individuals undergoing resequencing to dissect this trait's genetic architecture. The estimated heritability of ISKNV resistance was 0.187 ± 0.060. From 1,392,678 high-quality single nucleotide polymorphisms (SNPs), two significant SNPs (17_3,872,606 and 17_3,873,247) were identified, explaining 3.36% and 3.29% of phenotypic variance, respectively. Functional enrichment analysis revealed candidate genes were involved in neural signaling, metabolic processes, and MAPK signaling pathways, shedding light on the molecular basis of ISKNV resistance in Siniperca chuatsi. These findings not only illuminate the genetic basis of ISKNV resistance in Siniperca chuatsi, but also lay a foundation for subsequent genomic selection in aquaculture.

鳜鱼（sininiperca chuatsi）是中国最具经济价值的水生物种之一，但其养殖受到传染性脾肾坏死病毒（ISKNV）的严重威胁。通过遗传改良增强抗病能力是至关重要的，但该物种中ISKNV抗性的遗传基础仍然知之甚少。在这里，我们进行了一项全基因组关联研究（GWAS），对511名易感个体和223名抗性个体进行了重测序，以剖析该性状的遗传结构。ISKNV抗性遗传力估计为0.187±0.060。从1,392,678个高质量单核苷酸多态性（snp）中，鉴定出两个显著snp(17_3,872,606和17_3,873,247)，分别解释了3.36%和3.29%的表型变异。功能富集分析显示候选基因参与神经信号、代谢过程和MAPK信号通路，揭示了翘嘴鳜抗ISKNV的分子基础。这些发现不仅阐明了翘嘴鳜抗ISKNV的遗传基础，也为后续的水产基因组选择奠定了基础。

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引用次数: 0

A warning about the double-edged sword of integrated aquaculture: Mangrove degradation ongoing in Bac Lieu, Vietnam, as a case study 对综合水产养殖双刃剑的警告：以越南北Lieu的红树林退化为例

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-19 DOI: 10.1016/j.aquaculture.2026.743680

Tran Van Viet , Wen-Pei Tsai , Hoang Huy Huynh

Mangroves play a vital role in coastal protection, biodiversity, and aquatic habitats; however, their rapid decline continues under intense human pressure. This study identifies key drivers of mangrove degradation in Bac Lieu Province, Vietnam, with emphasis on aquaculture expansion and related socio-economic factors. Data were collected from March 2023 to March 2024 using a combination of quantitative and qualitative methods. Surveys covered 150 aquaculture households to document farm size, yields, and income. Structured interviews with 30 local loggers and 30 fishers provided additional insights into forest use and livelihood conditions. Statistical analysis showed that small-scale aquaculture, often pursued to compensate for low income from allocated plots, was the major cause of mangrove loss. Additional pressures came from wood harvesting, root-damaging fishing practices, and illegal land encroachment. Although integrated mangrove-shrimp systems are viewed as a sustainable model, the approach remains vulnerable due to weak governance, limited socio-economic support, and persistent policy gaps. Current land-use allocations restrict aquaculture development, prompting forest clearing as communities seek to expand income sources. Poverty, limited education, and few alternative livelihoods further reinforce short-term decisions that undermine long-term mangrove conservation. Findings highlight the need for policy reform, improved governance, and better alignment between economic impulses and conservation goals. Without such measures, integrated aquaculture systems may continue to cause mangrove degradation. The case of Bac Lieu provides lessons for broader efforts to balance mangrove protection and sustainable livelihoods.

红树林在海岸保护、生物多样性和水生栖息地方面发挥着至关重要的作用；然而，在巨大的人类压力下，它们的迅速减少仍在继续。本研究确定了越南北寮省红树林退化的主要驱动因素，重点是水产养殖扩张和相关的社会经济因素。数据收集时间为2023年3月至2024年3月，采用定量与定性相结合的方法。调查覆盖了150户水产养殖户，记录养殖场规模、产量和收入。对30名当地伐木工人和30名渔民的结构化访谈提供了对森林利用和生计条件的进一步了解。统计分析表明，往往为了补偿分配地块的低收入而进行的小规模水产养殖是红树林损失的主要原因。额外的压力来自木材采伐、破坏根系的捕鱼行为和非法侵占土地。尽管红树林-虾综合系统被视为一种可持续模式，但由于治理薄弱、社会经济支持有限以及持续存在的政策差距，这种方法仍然很脆弱。目前的土地使用分配限制了水产养殖业的发展，促使社区在寻求扩大收入来源时砍伐森林。贫困、有限的教育和很少的替代生计进一步加剧了破坏红树林长期保护的短期决定。研究结果强调了政策改革、改善治理以及更好地协调经济冲动和保护目标的必要性。如果不采取这些措施，综合水产养殖系统可能会继续造成红树林退化。Bac Lieu的案例为平衡红树林保护和可持续生计的更广泛努力提供了经验教训。

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引用次数: 0

Recovery of Edwardsiella tarda and Edwardsiella piscicida in freshwater fishes and assessment of their pathogenicity in Nile tilapia (Oreochromis niloticus) 淡水鱼中迟缓爱德华菌和淡水鱼爱德华菌的恢复及其对尼罗罗非鱼的致病性评价

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-01-19 DOI: 10.1016/j.aquaculture.2026.743671

Onanong Charoenwai , Ha Thanh Dong , Vuong Viet Nguyen , Satid Chatchaiphan , Saengchan Senapin , Patharapol Piamsomboon

Edwardsiellosis, a bacterial disease affecting global aquaculture, is caused by several species in the genus Edwardsiella. This study examined cases of Edwardsiella infections in multiple fish hosts from intensive aquaculture farms in Thailand during 2021–2022. Twenty putative Edwardsiella isolates were identified, comprising eighteen E. tarda isolates from Nile tilapia Oreochromis niloticus (11 isolates), red tilapia Oreochromis sp. (6 isolates), and snakehead fish Channa striata (1 isolate). In addition, two E. piscicida isolates were identified from red tilapia and barramundi Lates calcarifer. Antimicrobial susceptibility testing using disc diffusion assays with eight classes of antibiotics revealed multiple antibiotic resistance (MAR) indices ranging from 0.22 to 0.71. Representative E. tarda and E. piscicida isolates were selected to assess their pathogenicity in juvenile Nile tilapia. The cumulative mortality of Nile tilapia intraperitoneally injected with E. tarda (10⁷ CFU/fish) was 60%, while injection of E. piscicida at the same dose resulted in 100% mortality within seven days post-challenge. Grossly, moribund fish displayed diffuse pinpoint white spots on the pale liver and spleen. Significant histopathological alterations were noted in the liver and spleen, characterized by tissue degeneration, cellular disassociation, necrosis, intracellular bacterial colonization, and granuloma formation. No appreciable difference in the clinical manifestations was observed in challenged fish among the two Edwardsiella species. In conclusion, the present study reports evidence of pathogenic E. tarda and E. piscicida infections in freshwater fishes, underscoring the importance of implementing effective disease prevention and control measures.

爱德华氏菌病是一种影响全球水产养殖的细菌性疾病，由爱德华氏菌属的几种物种引起。本研究调查了2021-2022年期间泰国集约化水产养殖场多种鱼类宿主的爱德华氏菌感染病例。从尼罗罗非鱼（Oreochromis niloticus）（11株）、红罗非鱼（Oreochromis sp.）（6株）和黑头鱼（Channa striata）（1株）中分离出18株疑似爱德华氏菌。此外，从红罗非鱼和barramundi Lates calcarifer中分离出2株piscicida。采用纸片扩散法对8类抗生素进行药敏试验，多重抗生素耐药指数在0.22 ~ 0.71之间。选取具有代表性的迟缓E.和淡鱼E.分离株，对其在尼罗罗非鱼幼鱼中的致病性进行了评价。尼罗罗非鱼腹腔注射迟发绦虫（107 CFU/条）的累计死亡率为60%，而相同剂量注射淡化鱼绦虫在攻毒后7天内的死亡率为100%。肉眼可见，垂死的鱼在苍白的肝脏和脾脏上可见弥漫性的针状白色斑点。肝脏和脾脏有明显的组织病理学改变，表现为组织变性、细胞分离、坏死、细胞内细菌定植和肉芽肿形成。两种爱德华氏菌攻毒鱼的临床表现无明显差异。总之，本研究报告了淡水鱼中存在致病性迟缓E.和淡水鱼E.感染的证据，强调了实施有效疾病预防和控制措施的重要性。

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