Aquaculture最新文献

{"title":"Transcriptome-based investigation on the mechanism of bacterial inhibition of Aeromonas hydrophila by tea tree oil","authors":"Yachao Wang ,&nbsp;Yilei Liang ,&nbsp;Qing Jiang ,&nbsp;Qunlan Zhou ,&nbsp;Jiang Li ,&nbsp;Bo Liu","doi":"10.1016/j.aquaculture.2025.742259","DOIUrl":"10.1016/j.aquaculture.2025.742259","url":null,"abstract":"<div><div><em>Aeromonas hydrophila</em> is one of the major conditional pathogens causing loss in freshwater aquaculture, with strong drug resistance. Tea tree oil (TTO) can be a good alternative to antibiotics to achieve bacteriostatic effect against <em>A. hydrophila</em>. In this study, an in vitro bacterial inhibition assay was used to evaluate the inhibitory mechanism of TTO against <em>A. hydrophila</em> by detecting the minimum inhibitory concentration (MIC), growth and inhibition curves, cell membrane integrity, bacterial death, and transcriptome sequencing analysis. The results showed that the MIC of TTO against <em>A. hydrophila</em> was 39 μg/mL. Meanwhile, TTO significantly altered the cell membrane permeability and biofilm formation, which in turn increased the concentration of intracellular K⁺ and DNA exocytosis, and ultimately led to the death of <em>A.hydrophila</em>. Transcriptional sequencing revealed that TTO significantly altered both bacterial chemotaxis and two-component system (TCS) pathways in KEGG enrichment (<em>P</em> &lt; 0.05), which are related to intracellular survival, growth, motility, virulence and environmental stress response. In addition, Outer membrane porin (<em>OmpA</em>), serine protease (<em>ahpA</em>), repeat in toxin A (<em>rtxA</em>), temperature-sensitive Protease (<em>eprCAI</em>), adhesin (<em>aha</em>) and aerolysin (<em>aerA</em>) mRNA levels decreased (<em>P</em> &lt; 0.05). In summary, 1 MIC TTO can prevent bacterial colonisation in host tissues by disrupting the integrity of the membrane structure of <em>A. hydrophila</em>, reducing the activity and environmental resistance of serine proteases and other extracellular proteases to prevent bacterial colonisation, and thus reducing the survivability of <em>A. hydrophila</em> to achieve its bacteriostatic effect. It will provide a theoretical basis for the wide application of TTO in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742259"},"PeriodicalIF":3.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Pacific abalone progeny from cryopreserved sperm: Molecular insights into growth, reproduction, and immunity","authors":"Shaharior Hossen,&nbsp;Zahid Parvez Sukhan,&nbsp;Yusin Cho,&nbsp;Md Abu Hanif,&nbsp;Doo Hyun Cho,&nbsp;Kang Hee Kho","doi":"10.1016/j.aquaculture.2025.742290","DOIUrl":"10.1016/j.aquaculture.2025.742290","url":null,"abstract":"<div><div>This study aimed to produce market-sized Pacific abalone, <em>Haliotis discus hannai</em> progeny from cryopreserved sperm (PCS) and assess their mRNA stability. Embryonic development in PCS was moderately delayed until the veliger larval stage compared to control progeny (CP), although both groups exhibited similar morphological structures. Shell width (SW) and shell height (SH) in PCS and CP remained consistent throughout the experimental periods. However, the shell length (SL) and body weight (BW) of PCS were significantly lower than those of CP during the early developmental stages (up to 9 months). After this period, PCS and CP exhibited comparable body traits at 18 months and 3 years. At 9 months, the mRNA expression patterns of growth-associated genes (<em>Hdh-HGAP, Hdh-IGFBP7, Hdh-MSTN</em>), shell-forming genes (<em>Hdh-PRLSTN,</em> and <em>Hdh-SMP5</em>), immune regulatory genes (<em>Hdh-CAT, Hdh-GR, Hdh-GPx, Hdh-Caspase 3</em>, <em>Hdh-BAX,</em> and <em>Hdh-BCL2</em>) were similar between PCS and CP. However, <em>Hdh-MGF, Hdh-SOD, Hdh-IL17,</em> and <em>Hdh-NF-kB</em> exhibited significantly lower mRNA expression, whereas <em>Hdh-MIRP3</em> showed significantly higher expression in PCS compared to CP at 9 months. Furthermore, the expression patterns of all examined genes were similar in both PCS and CP at 3 years. In summary, PCS initially demonstrated lower growth traits (SL, BW) and impaired mRNA stability until 9 months; however, these traits normalized over time, resulting in comparable growth traits and gene expression to CP by 3 years. These findings suggest that cryopreserved sperm can effectively produce viable offspring with normal growth traits and mRNA stability for vital growth (<em>Hdh-MGF</em>, <em>Hdh-HGAP</em>, <em>Hdh-IGFBP7</em>, <em>Hdh-MIRP3</em> and <em>Hdh-MSTN</em>), shell formation (<em>Hdh-SMP5</em> and <em>Hdh-PRLSTN</em>), reproduction (<em>Hdh-GnRH</em>, <em>Hdh-GnRH-R</em>, <em>Hdh-APGWamide</em> and <em>Hdh-5HT</em>), and immune (<em>Hdh-SOD</em>, <em>Hdh-CAT</em>, <em>Hdh-GR</em>, <em>Hdh-GPx</em>, <em>Hdh-BCL2</em>, <em>Hdh-BAX</em>, <em>Hdh-Caspase 3</em>, <em>Hdh-IL17</em> and <em>Hdh-NF-kB</em>) associated genes.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742290"},"PeriodicalIF":3.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobubbles of oxygen and ozone are effective tools for pathogen treatment and for improving the survival rates of common carp larvae (Cyprinus carpio) – A case study","authors":"Vu Thi Trang ,&nbsp;Pham Thai Giang ,&nbsp;Vlastimil Stejskal ,&nbsp;Elayaraja Sivaramasamy ,&nbsp;Pham Thi Thanh ,&nbsp;Nguyen Thi Nguyen ,&nbsp;Phan Trong Binh ,&nbsp;Dang Thi Lua","doi":"10.1016/j.aquaculture.2025.742292","DOIUrl":"10.1016/j.aquaculture.2025.742292","url":null,"abstract":"<div><div>Nanobubble technology has demonstrated significant potential in enhancing the effectiveness of oxygen and ozone for water treatment and pathogen control. This study identifies the potential application of oxygen and ozone nanobubbles in improving the hatching efficiency of common carp eggs. Preliminary results indicated that an O₃NB concentration of 0.5 mg/L was effective in treating <em>Saprolegnia</em> spp. However, concentrations of 0.1–0.3 mg/L caused severe gill damage and significant mortality in fish larvae. Therefore, in this study, O₃NB was applied to fish embryos at 24 h post-hatch at concentrations of 0.46 ± 0.04 to 0.55 ± 0.06 mg/L for 1 h (T1), 2 h (T2), and 3 h (T3). Following this, from 48 h post-hatch, oxygen nanobubbles (O₂NB) were used to maintain optimal oxygen levels (9.98 ± 0.10 to 12.12 ± 1.04 mg/L) for common carp hatching. Embryo development and the presence of pathogens were assessed at 6, 24, 48, and 72 h. The results showed that the administration of O₃NB had no negative effect on the development of carp embryos. No <em>Saprolegnia</em> spp., <em>Dermocystidium</em> spp., and <em>Fragilaria</em> spp. were observed on embryos treated with O₃NB. In the control group, infection rates were 12.67 %, 3.33 %, and 73 %, respectively. At 72 h, the T1, T2, and T3 treatments had decreased rates of gill fungus infection (13.67 ± 5.51 %, 7.67 ± 0.58 %, and 5.33 ± 1.15 %, respectively) compared to the control (84.67 %, <em>P</em> &lt; 0.05). The larvae survival rates after 72 h in the three treatments using O₂NB and O₃NB were higher compared to the control, with the T2 group achieving the highest effectiveness at 94.07 ± 0.90 %. Our findings indicate that the application of O₂NB and O₃NB at appropriate developmental stages improves hatching efficiency in common carp.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742292"},"PeriodicalIF":3.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hybridization on growth, nutrition and reproductive potentiality of the Pacific oyster: Examination of annual variation in phenotypic and biochemical composition","authors":"Yuanxin Liang ,&nbsp;Jiafeng Fang ,&nbsp;Chengxun Xu ,&nbsp;Qi Li","doi":"10.1016/j.aquaculture.2025.742288","DOIUrl":"10.1016/j.aquaculture.2025.742288","url":null,"abstract":"<div><div>The Pacific oyster (<em>Crassostrea gigas</em>) is a key aquaculture species, contributing significantly to global marine production. Hybridization between different populations or strains of <em>C. gigas</em> can significantly improve its growth, survival or stress tolerance. However, the effects of hybridization on biochemical composition and reproductive potentiality were poorly understood. In this study, the growth, nutrient variations and reproductive potentiality of “Haida No. 1” (HH) line, Orange-shell line (OO) and their hybrids (OH, OO ♀ × HH ♂) of <em>C. gigas</em> were comprehensively investigated from February 2021 to January 2022. The shell height and living weight of OH were significantly higher than those of HH and OO throughout the year, except for the first four months, showing high-parental heterosis ranging from 7.58 % to 16.22 % for shell height and 6.97 % to 13.47 % for living weight, respectively. Glycogen and lipid decreased significantly after gamete discharge (summer) and gradually accumulated when the gonads were inactive (winter) in the four tissues. However, the protein content did not undergo significant alterations during either the reproductive or non-reproductive periods in the gill and muscle. In addition, the glycogen content and lipid content in the mantle and gonad-visceral mass of OH were higher than those of HH and OO, and the protein content consistently exceeded 50 % of dry weight in all three lines. All three of these <em>C. gigas</em> lines can be considered as high-quality food sources. The hybrid line (Orange-shell line ♀ × “Haida No. 1” ♂) of <em>C. gigas</em> appears to be the most nutritionally valuable variety. Moreover, the lagging gonadal development was not identified in the hybrid population (OH) compared to the inbred population (OO). Furthermore, the reproductive efforts and hatching rates in OH were significantly higher than those in OO, implying depression of reproductive potential as a consequence of inbreeding can be abolished or even enhanced through hybridization. Our study provides evidence that hybridization can improve the growth and nutritional quality of oysters, as well as ameliorate the deterioration of reproductive capacity caused by inbreeding, which can help us to better understand and employ hybridization in oyster aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742288"},"PeriodicalIF":3.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic assessment of feed efficiency in the spotted Babylon Babylonia areolata","authors":"Linwei Nie ,&nbsp;Yijie Kong ,&nbsp;Ziyi Ma ,&nbsp;Jingqiang Fu ,&nbsp;Shengtai Liu ,&nbsp;Xuan Luo ,&nbsp;Weiwei You ,&nbsp;Caihuan Ke","doi":"10.1016/j.aquaculture.2025.742284","DOIUrl":"10.1016/j.aquaculture.2025.742284","url":null,"abstract":"<div><div>The spotted Babylon (<em>Babylonia areolata</em>) is an emerging aquaculture species in China. Feed accounts for 40–60 % of the total expense of spotted Babylon rearing, making feed efficiency one of the most important traits for genetic improvement in this species. This study evaluated the growth and feed efficiency of 528 spotted Babylons from 22 families for 56 days, comprising two periods: the initial 1–28 days and days 29–56. The feed efficiency ratio (FER) and residual feed intake (RFI) displayed genetic correlations in both periods, with heritability values of 0.20 ± 0.077 and 0.14 ± 0.064, respectively, indicating medium and low heritability, respectively. The RFI had a high genetic and phenotypic correlation with the daily feed intake (DFI), and the FER had a high genetic and phenotypic correlation with the specific growth rate (SGR) and a high genetic correlation with the DFI. A comparative experiment was conducted on 11 families under the group feeding mode. The results showed that there were no significant differences in feed efficiency, growth traits, or feeding traits between the two modes. The study established an evaluation method for determining the feed efficiency of the spotted Babylon using a single individual rearing mode and found that spotted Babylon feed efficiency exhibited good breeding potential.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742284"},"PeriodicalIF":3.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA damage may cause the reproductive differences between the male tetraploid fish and diploid red crucian carp","authors":"Haitao Zhong ,&nbsp;Hong Chen ,&nbsp;Mingli Liu ,&nbsp;Chiye Zhao ,&nbsp;Chaoying Luo ,&nbsp;Zheduo Xiong ,&nbsp;Yan Li ,&nbsp;Yilin Wu ,&nbsp;Yuheng Wang ,&nbsp;Chun Zhang ,&nbsp;Chang Wu ,&nbsp;Qizhi Liu ,&nbsp;Yu Sun ,&nbsp;Shi Wang ,&nbsp;Ming Wen ,&nbsp;Fangzhou Hu ,&nbsp;Shaojun Liu","doi":"10.1016/j.aquaculture.2025.742283","DOIUrl":"10.1016/j.aquaculture.2025.742283","url":null,"abstract":"<div><div>Tetraploid fish are valuable diploid sperm donors and vertebrate models, and their reproductive characteristics have been widely attracted. In previous study, we obtained a new tetraploid fish (4nNT, 4n = 200, AAAB) derived from a cross between female autotetraploid fish (4nAU, 4n = 200, AAAA) and male allotetraploid fish (4nAL, 4n = 200, AABB). Compared with the original parent red crucian carp (RCC, 2n = 100, AA), a high proportion of abnormal sperm was observed in the semen of 4nNT. This study was conducted to gain further insight into the reproductive potential and fertility mechanisms of male 4nNT. During meiosis prophase, 100 pairs of bivalents were observed in germ cells of 4nNT, implying a doubling of crossover events in individual spermatocytes. The sperm concentration, motility (MOT), average path velocity (VAP), straight-line velocity (VSL), and curvilinear velocity (VCL) of RCC were significantly higher than 4nNT, and the lifetime was significantly shorter than 4nNT. In the artificial insemination test, higher fertilization and hatching rates were observed in groups using RCC as sperm donors, and higher abnormality rate were observed in groups using 4nNT as sperm donors. Regarding sperm DNA integrity, the higher comet rate was observed in 4nNT sperm, which may contribute to their lower hatching rate and higher abnormality rate. Transcriptome data indicated that the upregulated genes identified in the testis of 4nNT were significantly enriched in 14 signaling pathways, including the homologous recombination, fanconi anemia pathway, p53 signaling pathway and apoptosis, which are link to DNA damage and repair. This indicates the 4nNT spermatocytes may be unable to fully withstand the DNA damage repair pressure caused by the doubling of crossover events. In conclusion, this study suggests that the reproductive differences between 4nNT and RCC may be caused by DNA damage, our findings provide novel insights into the fertility mechanism of male 4nNT, which is of great significance for improving its fertility, population reproduction, and efficient production of triploids.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742283"},"PeriodicalIF":3.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of green LED-accelerated wound healing in olive flounder (Paralichthys olivaceus)","authors":"Ju-Yeop Lee ,&nbsp;Yoonhang Lee ,&nbsp;Jiyeon Park ,&nbsp;Hyo-Young Kang ,&nbsp;Young Ung Heo ,&nbsp;Ahran Kim ,&nbsp;Nameun Kim ,&nbsp;HyeongJin Roh ,&nbsp;Do-Hyung Kim","doi":"10.1016/j.aquaculture.2025.742267","DOIUrl":"10.1016/j.aquaculture.2025.742267","url":null,"abstract":"<div><div>Skin injuries of aquatic animals are common and often cause serious problems as a damaged barrier cannot maintain osmotic balance and homeostasis. A few studies have reported that particular wavelengths green light can promote wound healing in mammals and fish. However, Although Light emitting diode (LED) illumination has been applied to cure skin wound in mammals, little is known about wound healing responses in fish. The purposes of this study, therefore, were to understand healing process of skin wound of fish exposed to green LED using transcriptional data. Sixty skin-wounded olive flounder in each group were exposed to ambient, white LED (400–700 nm), blue LED (465 nm), and green LED (520 nm) light in 12-h light/ dark cycles for 28 days. Fish were observed at 28 days post injuries (dpi) to examine for wound closure. For transcriptome analysis, fish in ambient, white LED, and green LED groups were sacrificed to obtain tissue samples from the wounded area at 3 and 7 dpi. As a result, there were no significant differences between groups in survival rate for a month. However, the size of wounds of fish in the green light exposed-group was significantly reduced followed by white and blue LED groups, and ambient light group. Fish in the green LED group showed up-regulation of many genes involved in electron transfer systems and ATP/ROS production in fish. This indicates that green LED photons might be absorbed as energy in cells in the fish skin including chromophores. Those factors further activate signal pathways such as JAK/STAT, FoxO and MAPK to transmit signals to cell division and stimulating various growth factors, which are necessary for accelerating wound healing. This study demonstrates that the illumination of green LED can accelerate wound healing and will contribute to a better understanding of phototherapy and healing mechanism in fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742267"},"PeriodicalIF":3.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soybean meal-refined treatment mitigated high soybean meal diet-induced oxidative damage in the gut of crayfish via microbial metabolic function remodeling","authors":"Minglang Cai ,&nbsp;Chuang Shao ,&nbsp;Zhigang He ,&nbsp;Ruibing Chang ,&nbsp;Huajun Zhang ,&nbsp;Yi Hu","doi":"10.1016/j.aquaculture.2025.742286","DOIUrl":"10.1016/j.aquaculture.2025.742286","url":null,"abstract":"<div><div>The high cost and unreliable supply of fishmeal has stimulated concern over low fishmeal feed, making soy-derived protein receive more attention recently. However, dietary supplementation with high soybean meal (SBM) negatively affects host growth, health status, and gut microbial metabolic functions, encouraging the search for novel soy-derived protein sources to replace SBM. Accordingly, this experiment was designed to investigate the effects of replacing SBM with fermented soybean meal and soy protein concentrate on the growth, gut histomorphology, antioxidant capacities, and non-specific immunity of crayfish, together with the underlying metabolic mechanisms of microbial regulation. 600 crayfish (4.00 g) were randomized into four treatments (FM, SBM, FSM, and SPC) and fed diets with fishmeal and three different soy-derived proteins for six weeks. The present findings revealed that dietary FSM and SPC inclusion mitigated the growth decline and intestine injury caused by the SBM diet, as evidenced by the well-developed villus, epithelium, and dense connective tissue, as well as lower hemolymph malondialdehyde and lactic acid contents (<em>P</em> &lt; 0.05). Moreover, crayfish supplemented with FSM and SPC diets experienced enhanced immunity compared to the SBM group, together with the increased hemolymph lysozyme and acid phosphatase levels (<em>P</em> &lt; 0.05), and the decreased <em>nfkb</em> and <em>alf</em> mRNA expression levels (<em>P</em> &lt; 0.05). Also, crayfish given FSM and SPC diets exhibited increases in the intestine glutathione content and <em>nrf2</em> mRNA expression level (<em>P</em> &lt; 0.05). Furthermore, dietary FSM and SPC supplementation eliminated SBM-induced microbial dysbiosis and dysfunction, characterized by the decreased <em>Citrobacter</em> and <em>Anaerorhabdus</em> and increased <em>RsaHf231</em>. Further findings revealed that the identified 88 differential microbes and 66 differential metabolites were shared between the SBM group and the FM and SBM-refined groups, with <em>Candidatus</em> Bacilloplasma, <em>Cyanobium PCC-6307,</em> and <em>Vogesella</em>, and phosphatidylcholine being the main contributors to crayfish health. Moreover, dietary SBM-refined treatment contributed to more robust and complicated microbial co-occurrence networks and activated amino acid metabolism-related pathways. To conclude, dietary FSM and SPC supplementation reversed the weakened immunity and antioxidant capacities of crayfish given a soybean meal diet by reshaping microbial metabolic functions, which in turn improved the growth and gut histomorphological structure.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742286"},"PeriodicalIF":3.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medicinal plant-product encapsulated with herb-hydrogel for ammonia removal and stress alleviation in semi-biofloc system containing aquaponics water cultured with Pangasianodon hypophthalmus","authors":"Kalpana Arambam ,&nbsp;Kishore Kumar Krishnani ,&nbsp;Rajkumar Debarjeet Singh ,&nbsp;Ajit Kumar Verma ,&nbsp;Kaushik Banerjee ,&nbsp;Thongam Ibemcha Chanu ,&nbsp;Neeraj Kumar","doi":"10.1016/j.aquaculture.2025.742285","DOIUrl":"10.1016/j.aquaculture.2025.742285","url":null,"abstract":"<div><div>The study investigates the use of microencapsulated product of Indian gooseberry (<em>Phyllanthus emblica</em>) developed using hydrogel derived from isabgol (<em>Plantago ovata</em>) for ammonia removal in aquaponics water and the growth performance of <em>Pangasianodon hypophthalmus</em> for a 45-day period. The study consists of a control (C) and two treatments: T1 (isabgol) and T2 (isabgol+Indian gooseberry). Ammonia was spiked weekly at varying concentrations. T2 showed significantly highest (<em>p</em> &lt; 0.05) ammonia removal in all the different spiking dosages, followed by T1. Ammonia was eliminated more rapidly in the 4th week. T2 removed 83.24 ± 0.37 % of ammonia in 36 h and 95.27 ± 0.59 % in 48 h. The decline in pH levels brought on by the nitrification process is connected with less ammonia removal in the 5th week. The fish growth indices (final total biomass, net weight gain, specific growth rate) and feed conversion ratio were significantly (<em>p</em> &lt; 0.05) improved in treatment groups, with T2 exhibiting superior performance. The hematological and serum biochemical analyses indicated reduced stress in treatment groups compared to the control. The characterization of isabgol and gooseberry also revealed their potential as bioactive carriers. LC-MS analysis confirmed the presence of oxy-chromen-4-one, 4-indolecarbaldehyde, kaempferol, and apigenin in isabgol. Phenolic acid, flavonoids, essential amino acids, and vitamins were found in Indian gooseberry. The study revealed that the developed product has the potential to decontaminate high ammonia levels, mitigate stress, and improve the growth performance of <em>P hypophthalmus</em>, which can be attributed to the controlled release and immunostimulating effect of microencapsulated Indian gooseberry product coupled with semi-biofloc formation facilitated by hydrogel derived from isabgol.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742285"},"PeriodicalIF":3.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel C-type lectin 4E from Cromileptes altivelis (CaCTL4E) participates in antibacterial innate immunity","authors":"Zhenjie Cao ,&nbsp;Zhiru Liu ,&nbsp;Jinying Zhang ,&nbsp;Jing Zhang ,&nbsp;Qingyu Wu ,&nbsp;Aie Kang ,&nbsp;Ying Wu ,&nbsp;Yongcan Zhou ,&nbsp;Yun Sun","doi":"10.1016/j.aquaculture.2025.742282","DOIUrl":"10.1016/j.aquaculture.2025.742282","url":null,"abstract":"<div><div>C-type lectins (CTLs) play a crucial role as pattern recognition receptors (PRRs) in the innate immune system. Nevertheless, the underlying mechanisms of CTL-mediated immune recognition and response in bony fish remain poorly understood. In the current study, a CTL member, namely CaCTL4E, was characterized in <em>Cromileptes altivelis</em>, and its biological functions and roles in response to bacterial infection were analyzed. The CaCTL4E gene encodes 254 amino acids with a 765 bp coding sequence and exhibits typical C-type lectin structural features, including a carbohydrate recognition domain (CRD), six conserved cysteine residues, a WIGL motif, and a predicted mannose-binding EPN motif. In healthy <em>C. altivelis</em>, <em>CaCTL4E</em> was differentially expressed in all tested tissues, with a predominant expression in the liver. Notably, upon <em>Vibrio harveyi</em> stimulation, the expression of CaCTL4E in the spleen and head kidney was significantly upregulated, indicating its active involvement in the immune response. The recombinant CaCTL4E (rCaCTL4E) exhibited the ability to agglutinate red blood cells and four bacteria (<em>V. harveyi</em>, <em>Edwardsiella tarda</em>, <em>Streptococcus agalactiae</em>, and <em>Staphylococcus aureus</em>), as well as to bind these bacteria and four pathogen-associated molecular patterns (PAMPs: lipopolysaccharide, peptidoglycan, mannose, and galactose). Interestingly, the binding of rCaCTL4E to PAMPs competitively inhibited its bacterial binding and agglutination activities. Moreover, rCaCTL4E directly inhibited the growth of the four bacteria <em>in vitro</em>. <em>In vivo</em>, the overexpression or knockdown of CaCTL4E led to a decrease or increase in the bacterial burden, respectively. Importantly, rCaCTL4E also exhibited immunomodulatory properties by enhancing the phagocytic activity of head kidney macrophages. These results provide evidence that CaCTL4E acts as the important PRR, and lay a foundation for further understanding the process of CaCTL4E participating in the immune response to pathogenic infections.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"601 ","pages":"Article 742282"},"PeriodicalIF":3.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}