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The Effect of Extract Turmeric (Curcuma longa) on Spermatozoa Motility in Fresh Semen of Goats 姜黄提取物对山羊新鲜精液中精子活力的影响
Pub Date : 2023-05-09 DOI: 10.9734/ajob/2023/v18i2339
S. Sitepu, J. Marisa, M. Farid
Aims: Determine the motility of spermatozoa in fresh goat semen after being given turmeric extract. Study Design:  Randomized Block Design. Place and Duration of Study: Sample: Galang, Deli Serdang Regency, Indonesia, between December 2022 and January 2023. Methodology: Giving turmeric extract to the goat once a day. The treatment included adding 0ml, 25 ml, 50 ml, 75 ml, and 100 ml turmeric extract. The parameter observed was the percentage of motility spermatozoa in fresh goats semen. The preparation of turmeric extract, Feeding goats using turmeric extract every day at 08.00, collection of fresh semen, evaluating motility spermatozoa. This  research obtained  conducted  using  a  Randomized  Block  Design  consisting  of  5  treatment  levels  and  five replications. Semen storage using 30 Bucks, which has done for three days. Results: The addition of turmeric extract 0 ml showed an motility value of 65%, 25 ml = 70%, 50 ml = 75%, 75 ml = 75% and 100 ml = 75%. The analysis of variance showed that the results give a real impact on the motility of spermatozoa. The results showed that the addition of turmeric extract had significant effect (P <0.01). Conclusion: Adding 50 ml of turmeric extract was the best result on spermatozoa motility in fresh goat semen.
目的:测定姜黄提取物对新鲜山羊精液中精子活力的影响。研究设计:随机区组设计。研究地点和时间:样本:加朗,Deli Serdang摄政,印度尼西亚,2022年12月至2023年1月。方法:每天给山羊一次姜黄提取物。处理包括加入0ml、25ml、50ml、75ml、100ml姜黄提取物。观察到的参数是新鲜山羊精液中活力精子的百分比。制备姜黄提取物,每天08.00用姜黄提取物饲喂山羊,采集新鲜精液,评价精子活力。本研究采用随机区组设计,包括5个处理水平和5个重复。用30美元储存精液,已经用了三天了。结果:加入0 ml的姜黄提取物,其运动值分别为65%、25 ml = 70%、50 ml = 75%、75 ml = 75%、100 ml = 75%。方差分析表明,该结果对精子的活力有真实的影响。结果表明,添加姜黄提取物有显著影响(P <0.01)。结论:添加50 ml姜黄提取物对新鲜山羊精液中精子活力的影响最佳。
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引用次数: 0
Food and Medicinal Aspects of Freshwater Snail Filopaludina bengalensis (Lamarck, 1822) 淡水蜗牛Filopaludina bengalensis的食物和药用方面(Lamarck, 1822)
Pub Date : 2023-05-06 DOI: 10.9734/ajob/2023/v18i2338
Subhalakshmi Rout, S. Rath, Sanjeet Kumar
Food is becoming more and more scarce due to several negative factors. People only focus on the readily available foods in their vicinity that are limited and well-known. Unexplored foods from the forest are out of our food baskets due to a metallic lifestyle that creates a lot of health problems and retards the development of a nation. From the forest’s wealth, plants are more known, but edible insects, snails, fish, and other such species are less known and need to be explored. Keeping this in view, an attempt has been made to find medicinal foods from the faunal wealth of Odisha. A survey was carried out in selected districts (Sundargarh, Mayurbhanj, Cuttack, and Keonjhar) of Odisha state in 2023 and enumerated the species from the faunal wealth having food and medicinal values. Results revealed that snails are most common and consumed as a nutraceutical and have fewer scientifically known taxa in medico-food aspects. In this communication, the food and medicinal uses of Filopaludina bengalensis, a freshwater snail, are discussed, and further advanced work is recommended to do value addition for sustainable utilization.
由于一些负面因素,食物变得越来越稀缺。人们只关注他们附近的食物,这些食物是有限的和众所周知的。由于金属的生活方式造成了许多健康问题,阻碍了一个国家的发展,森林中未开发的食物已经从我们的食物篮子中消失了。从森林的财富中,植物是众所周知的,但可食用的昆虫、蜗牛、鱼和其他这类物种却鲜为人知,需要探索。考虑到这一点,人们尝试从奥里萨邦丰富的动物中寻找药用食物。2023年,在奥里萨邦选定的地区(Sundargarh、Mayurbhanj、Cuttack和Keonjhar)进行了一项调查,并从动物财富中列举了具有食物和药用价值的物种。结果表明,蜗牛是最常见的营养食品,在医学食品方面科学已知的分类群较少。本文讨论了淡水蜗牛Filopaludina bengalensis的食品和药用价值,并建议进一步开展增值工作以实现可持续利用。
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引用次数: 2
Critical Evaluation of the Role of Lycopene on Reproduction of Male Wistar Rats Exposed to Dimethoate 番茄红素对暴露于乐果的雄性Wistar大鼠生殖作用的关键性评价
Pub Date : 2023-05-04 DOI: 10.9734/ajob/2023/v18i2337
Mary Odeh, A. Amachree, A. Obulor, E. E. Orlu
Aim: This study was aimed at evaluating the role of Lycopene on reproduction of male Wistar rats exposed to Dimethoate. Experimental Design: A completely randomized experimental design using standard methods for analysis.  Location and Duration of Study: This study was carried out in the Animal house, Department of Animal and Environmental Biology of Rivers State University, Nkpolu-Oroworukwo Port Harcourt, Nigeria. GPS 4o47'50''N 6o58'49''E. This study lasted for 21days.  Methodology: Thirty male rats were randomly selected into five (5) groups A-E (6 animals per group). Groups B, C and D were gavaged 10, 20, 30 mg/kg/bw/day of dimethoate respectively, and co-administered Lycopene at 10mg/animal daily. Group E were administered 30mg/kg/bw/day of dimethoate without Lycopene.  All animals were allowed access to cool clean water and standard rat pellet ad libitum. Twenty-four hours to the termination of the experiment, feed was withdrawn from the animals. Blood samples were collected by ocular puncture into heparinized tubes for hormonal analysis. Hormones such as Testosterone, Progesterone, estradiol, Follicle Stimulating Hormone (FSH) and luteinizing hormone (LH) were analysed based on the manufacturer’s instruction using Randox Monza assay kit. For Histopathological analysis of the testis, 0.5g of the testis was fixed in 10% formalin and sectioned with a digital Microtome (AO spencer No. 820) at 5μm thick. Histological sections were mounted on clean glass slides and stained with Hematoxylin and Eosin (H&E). The slides were viewed at X400 magnification (modified Orlu, 2014). Statistical analysis was carried out using one-way Analysis of Variance (ANOVA) and expressed as their respective units. Where significant differences were found, Pair-wise comparisons conducted with Tukey test using SPSS 22 software. Results: It was observed that the level of all hormone considered decreased significantly with an increase in Dimethoate exposure despite the administration of lycopene. However, administration of dimethoate only resulted in a significant (p<0.05) reduction in the concentration of all hormones compared with groups given lycopene. The massive cellular degeneration observed in the seminiferous epithelium of experimental animals exposed to higher concentrations (20 and 30mg/kg/bw/day) of Dimethoate is indicative of a targeted action of the pesticide on male reproductive organ. The antioxidant property of Lycopene is clearly visible in testicular cross section of animals in groups given lycopene as gradual regeneration of both mitotic and meiotic spermatogenic elements were evidence.
目的:探讨番茄红素对雄性Wistar大鼠接触乐果后生殖功能的影响。实验设计:采用标准方法进行分析的完全随机实验设计。研究地点和时间:本研究在尼日利亚哈科特港Nkpolu-Oroworukwo河流州立大学动物与环境生物系动物舍进行。GPS 4047 '50 "北纬6058 '49 "东经。试验期21d。方法:雄性大鼠30只,随机分为5组(A-E),每组6只。B、C、D组分别灌胃乐果10、20、30 mg/kg/bw/ D,同时以10mg/ D / D的剂量联合给予番茄红素。E组给予不含番茄红素的乐果果30mg/kg/bw/d。所有动物都可以随意使用清凉的清洁水和标准的大鼠颗粒。实验结束前24小时,停止给动物喂食。通过眼穿刺肝素化管采集血样进行激素分析。睾酮、孕酮、雌二醇、促卵泡激素(FSH)和黄体生成素(LH)等激素依据生产厂家说明书使用Randox Monza检测试剂盒进行分析。将0.5g睾丸固定在10%福尔马林中,用数字切片机(AO spencer No. 820)切片,切片厚度为5μm。组织切片贴于干净的玻片上,用苏木精和伊红(H&E)染色。以X400倍率观察载玻片(修改Orlu, 2014)。采用单因素方差分析(ANOVA)进行统计分析,并以各自单位表示。如发现有显著差异,采用SPSS 22软件进行两两比较,采用Tukey检验。结果:我们观察到,所有激素的水平考虑显著下降,尽管施用番茄红素的敌多畏暴露的增加。然而,与番茄红素组相比,给药乐果只导致所有激素浓度显著(p<0.05)降低。高浓度(20和30mg/kg/bw/d)的乐果对雄性生殖器官有靶向作用,实验动物精系上皮细胞大量变性。番茄红素在有丝分裂和减数分裂生精元素逐渐再生的动物睾丸横切面上显示出明显的抗氧化特性。
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引用次数: 0
Assessment of Plant Diversity: A Medicinal, Conservational and Environmental Study 植物多样性评估:药用、保护和环境研究
Pub Date : 2023-04-28 DOI: 10.9734/ajob/2023/v18i1336
Binodini Mishra
An assessment study of the plant diversity in the campus of Prananath College (Autonomous), Khordha was carried out during 2019-20 and a checklist was prepared. A total of 241 vascular plant species belonging to 72 different families were recorded including four species of Gymnosperms. Among the families, Fabaceae, Apocynaceae, Euphorbiaceae, Malvaceae, Lamiaceae and Poaceae were the dominating families of the vascular plants in the study area. Aquatic plants, medicinal plants and ornamental plants are included in the study area. The floristic composition also include occurrence of invasive alien species such as Parthenium, Ageratum, Cassia, Croton sparsiflorus. The study also gives attention towards the conservation of bio-resources of the campus, toxic effects of the plants along with their medicinal values, proper utilization of bio-wealth in research and academic activities. Documentation of flora check list will be helpful in the environmental study too.
在2019- 2020年期间,对科尔达Prananath学院(自治)校园的植物多样性进行了评估研究,并编制了清单。共记录到维管植物72科241种,其中裸子植物4种。其中,豆科、夹竹桃科、大戟科、锦葵科、兰科和禾本科是研究区维管植物的优势科。研究区包括水生植物、药用植物和观赏植物。植物区系组成还包括Parthenium、Ageratum、决明子、Croton sparsiflorus等外来入侵种。该研究还关注校园生物资源的保护,植物的毒性作用及其药用价值,在研究和学术活动中合理利用生物财富。植物区系检查表的编制也有助于环境研究。
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引用次数: 0
Morphometric Study of the Breast in Nulliparous Nigerian Females at the University of Port Harcourt, Rivers State, Nigeria 尼日利亚河州哈科特港大学对未产尼日利亚女性乳房的形态计量学研究
Pub Date : 2023-04-25 DOI: 10.9734/ajob/2023/v18i1335
E. I. Edibamode, I. Bob-Manuel, T. Hart
Background: The breast, also known as the mammary gland, is an essential aesthetic feature in a woman. Anthropometric breast measurements are vital for research and in reconstructive surgery as a quantitative tool for preoperative and postoperative evaluation. Aim: This study aimed to measure morphometric parameters of the breast of nulliparous Nigerian females to establish reference baseline data for the region. Methodology: In this prospective descriptive and analytical study, 66 female students from the University of Port-Harcourt in Rivers State, Nigeria, ranging in age from 16 to 30, were used. Various morphometric parameters were observed and measured using measuring tape, a metre rule, and skin markers in a standing position. Descriptive and inferential analysis of data was done using Statistical Package for Social Sciences (SPSS) version 23. Results: Most of the mean values of the measured anthropometric parameters of the left breast were higher than the right but displayed no significant difference except the nipple and mammary projections, which showed a significant difference between the right and left breasts. This study on the morphometry of the female breast revealed the slender and severe ptosis shapes as the most prevalent breast shapes, while the least prevalent were the bell and pseudoptosis breast shapes. The mammary size volume showed the left breast to be significantly bigger than the right, while of all the breast parameters analysed, the nipple projection (left) and mammary projection (right) displayed significant differences in this study, with all the right and left measured breast parameters indicating a positive correlation. Conclusion: The values from this study can be used as a guide by plastic surgeons providing services to females of African descent requiring aesthetic and reconstructive breast surgeries, as well as in physical anthropological studies and racial identification.
背景:乳房,也被称为乳腺,是女性必不可少的审美特征。人体乳房测量作为术前和术后评估的定量工具,对研究和重建手术至关重要。目的:本研究旨在测量尼日利亚未产女性乳房的形态计量参数,为该地区建立参考基线数据。方法:在这项前瞻性描述性和分析性研究中,研究对象是来自尼日利亚河流州哈科特港大学的66名女学生,年龄从16岁到30岁不等。使用卷尺、米尺和站立位置的皮肤标记物观察和测量各种形态计量参数。使用社会科学统计软件包(SPSS)第23版对数据进行描述性和推断性分析。结果:左乳人体测量参数的平均值大多高于右乳,但除乳头和乳腺突起外,其余均无显著差异,显示左右乳差异显著。对女性乳房形态的研究表明,细长和严重的上睑下垂是最常见的乳房形状,而最不常见的是钟形和假下垂乳房形状。乳腺尺寸体积显示左乳明显大于右乳,而本研究分析的所有乳腺参数中,乳头投影(左)和乳腺投影(右)在本研究中表现出显著差异,左右测量的乳腺参数均为正相关。结论:本研究的价值可以作为整形外科医生为需要进行乳房美容和重建手术的非洲裔女性提供服务的指导,以及在身体人类学研究和种族识别方面的指导。
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引用次数: 0
Relationship between Age and Lumbosacral Angle (LSA) of Adult Nigerians: A Cross-sectional Study 年龄与尼日利亚成年人腰骶角(LSA)关系的横断面研究
Pub Date : 2023-04-24 DOI: 10.9734/ajob/2023/v18i1334
I. Bob-Manuel, Oghenefego Michael Adheke
Background: The lumbosacral angle (LSA), is a key morphometric parameter important in the management of patients with low back disorders. Aim: The study evaluated the relationship between age and lumbosacral angle of adult Nigerians. Methods: Two hundred and twenty-six (226) radiographs (113 males to 113 females) were obtained from Radiology departments of Federal Medical Centre (FMC), Owerri. Inclusion criteria were, lateral radiographs of lumbosacral region marked above 18 years of ages and only clear X-ray films were used.Using the Ferguson's approach, the lumbosacral angle was measured. All data obtained were expressed both in descriptive and inferential statistics. Results: Both male and female categories showed a progressive increase in lumbosacral angle across age groups, but later decreased in the age group of 60 years and above. The mean lumbosacral angle of male subjects was 35.74° while that of females was 41.46°. There was a significant difference (t = 3.16, p = 0.002) between the lumbosacral angle of male and female categories. However, there was a weak, positive correlation between age and lumbosacral angle of males (r = 0.187) and females (r = 0.392). Conclusion: The males had a lower mean lumbosacral angle compared to the females. Age was significantly correlated with lumbosacral angle in both sexes.
背景:腰骶角(LSA)是腰背部疾病患者治疗中一个重要的形态学参数。目的:探讨尼日利亚成年人腰骶角与年龄的关系。方法:从奥韦里联邦医疗中心(FMC)放射科获得226张x线片(男113张,女113张)。纳入标准为:18岁以上腰骶侧位片,且仅使用清晰x线片。采用弗格森入路测量腰骶角。所有获得的数据均以描述性统计和推理统计两种方式表示。结果:男性和女性腰骶角在不同年龄组中均呈进行性增加,但在60岁及以上年龄组中呈下降趋势。男性腰骶角平均值为35.74°,女性腰骶角平均值为41.46°。男女两组腰骶角差异有统计学意义(t = 3.16, p = 0.002)。而年龄与腰骶角之间存在微弱的正相关关系,男性(r = 0.187),女性(r = 0.392)。结论:男性患者腰骶角平均值低于女性患者。男女腰骶角与年龄有显著相关。
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引用次数: 1
Metopism: Its Prevalence and Morphometry in Adult Nigerian Dry Skulls 异位:尼日利亚成人干颅骨的患病率和形态计量学
Pub Date : 2023-04-14 DOI: 10.9734/ajob/2023/v18i1333
E. I. Edibamode, I. Bob-Manuel, S. F. Bob-Manuel
Background: Metopic suture also known as the frontal, interfrontal, or median frontal suture is formed in the midline at the meeting of the two halves of the frontal bone. Normally it gets obliterated by early childhood, but in some cases, it persists and is described as metopism. This study is aimed at investigating metopism prevalence in dry Nigerian skull.  Methods: This study was conducted on ninety-six (65 males and 31 females) adult Nigerian dry skulls from 5 selected Universities in the Southern part of Nigeria. Metopic suture (metopism) was considered to be complete when it continued uninterruptedly from the nasion to the bregma and incomplete when it was not present over its entire length. The incomplete metopic sutures were further classified into shapes. Results: Metopism was observed in 31 skulls (32.3%) of which 22 (21.9%) were males and 9 (9.4%) were females. Complete metopic suture was found in only 1 skull (1.04%) and incomplete metopic suture was seen in 30 skulls (31.25%). Among the incomplete ones, linear shape metopic suture was the most common in 16 skulls (16.67%). 7 (7.29%) were V-shaped metopic suture and another 7 (7.29%) were double-type metopic suture. Prevelence of metopic suture was slightly higher in males than in females. However, there was no statistical level of association with respect to sexual dimorphism. Conclusion: The prevalence and morphometry of metopism in the 96 adult Nigerian skulls studied, revealed complete metopism to be 1.04% with a length of 127.0mm as against incomplete metopism of 31.25% making a total prevalence of 32.29%. The prevalence of metopic suture was slightly higher in males than in females with the linear metopic suture occurring the most. Sexual dimorphism with respect to incomplete metopism types and shapes revealed no association This study is important for radiologists and neurosurgeons in diagnosis of frontal bone fractures and during surgical intervention including frontal craniotomy.
背景:异位缝合也称为额、额间或额正中缝合,形成于额骨两半的中线交汇处。通常情况下,它会在儿童早期消失,但在某些情况下,它会持续存在,并被描述为反位性。本研究的目的是调查在干尼日利亚头盖骨上移的患病率。方法:选取尼日利亚南部5所大学的96例尼日利亚成年干颅骨(男65例,女31例)进行研究。异位缝合(metopism)从鼻窦持续到鼻窦时被认为是完整的,当它不存在于整个长度时被认为是不完整的。对不完全止位缝线进行进一步的形状分类。结果:31例(32.3%)颅骨出现异位,其中男性22例(21.9%),女性9例(9.4%)。完全异位缝合仅1例(1.04%),不完全异位缝合30例(31.25%)。在不完全缝合中,16例颅骨以线状异位缝合最为常见(16.67%)。v型异位缝合7例(7.29%),双型异位缝合7例(7.29%)。异位缝合在男性中的患病率略高于女性。然而,在两性二态性方面没有统计水平的关联。结论:对96例尼日利亚成人颅骨的异位率和形态测定结果显示,完全异位率为1.04%,长度为127.0mm;不完全异位率为31.25%,总患病率为32.29%。异位缝合在男性中的发生率略高于女性,其中线性异位缝合发生率最高。该研究对放射科医生和神经外科医生诊断额骨骨折以及包括额骨开颅术在内的手术干预具有重要意义。
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引用次数: 0
Effects of Light on the Growth and β-carotene Accumulation in the Green Algae Dunaliella salina 光照对盐藻生长和β-胡萝卜素积累的影响
Pub Date : 2023-04-12 DOI: 10.9734/ajob/2023/v18i1332
Quynh Anh Tran-Nguyen, T. V. Tran, M. Trinh-Dang
The marine microalgae Dunaliella salina holds significant economic value due to its rich content of β-carotene, a natural pigment with high antioxidant capacity, immune-stimulating properties, and a crucial role in antioxidant reactions with vitamins C and E. This study evaluated the effects of light factors on the growth and biosynthesis of β-carotene in the Dunaliella salina strain isolated from coastal waters in Central Vietnam. The microalgae strain was cultured in the static f/2 medium at 25°C, under different light spectra (blue, red, and white) and light intensities (13.5, 27.0, and 40.5 µmol photon/m2/s), with a similar light/dark cycle of 16/8. The results indicated that the microalgae strain showed the highest growth rate and production yield of β-carotene under the red LED light, whereas the highest β-carotene accumulation in each microalgal cell was obtained under the blue LED light. Regarding light intensity, the best growth and β-carotene production yield was observed under the highest experimental light intensity of 40.5 µmol photon/m2/s, but the highest β-carotene content per algal cell was reached under the lowest intensity (13.5 µmol photon/m2/s). These findings provide important scientific data for further research to identify the optimal conditions for increasing biomass and stimulating the accumulation of valuable secondary compounds of the microalgae Dunaliella salina in Vietnam.
海洋微藻盐杜氏藻(Dunaliella salina)富含β-胡萝卜素,是一种具有高抗氧化能力和免疫刺激特性的天然色素,在与维生素C和e的抗氧化反应中起着至关重要的作用,因此具有重要的经济价值。本研究评估了光照因素对从越南中部沿海水域分离的盐杜氏藻菌株β-胡萝卜素生长和生物合成的影响。微藻菌株在25°C的静态f/2培养基中,在不同光谱(蓝、红、白)和光强(13.5、27.0和40.5µmol photon/m2/s)下培养,光/暗周期相似,为16/8。结果表明,红色LED光下微藻菌株的生长速率和β-胡萝卜素产量最高,而蓝色LED光下各微藻细胞中β-胡萝卜素积累量最高。在光强方面,最高光强为40.5µmol光子/m2/s时,藻细胞的生长和β-胡萝卜素产量最佳,但最低光强为13.5µmol光子/m2/s时,每个藻细胞β-胡萝卜素含量最高。这些发现为进一步研究越南盐杜氏微藻增加生物量和促进有价值次生化合物积累的最佳条件提供了重要的科学数据。
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引用次数: 0
The Effect of Indomethacin on the Sperm Motility of Adult Wistar Rats 吲哚美辛对成年Wistar大鼠精子活力的影响
Pub Date : 2023-04-11 DOI: 10.9734/ajob/2023/v17i4330
L. Zitte, Prisca I. Uzodinma
Indomethacin is a non-steroidal anti-inflammatory drug (NSAID) used mostly in the treatment of pain, feverish condition, headaches and inflammation. This study was therefore carried out to evaluate the effect of indomethacin on the male reproductive system, focusing on sperm quality. Twenty male rats were divided into four groups, (groups1,2,3,and 4) with group one serving as control. The other three groups receiving different doses of indomethacin, (groups 2-4 receiving 1mg/kg, 2mg/kg and 4mg/kg respectively) for 21 days. After the period, sperm samples were collected from all the groups and analyzed to evaluate sperm quality. The sperm qualities analyzed include; sperm motility, semen viscosity, sperm morphology, sperm count, etc. the results of the study indicated that indomethacin had a negative impact on the sperm qualities of the rats, as it decreased sperm count and sperm motility , and had a detrimental effect on the overall sperm quality. This study provides important insights into the potential side effects of indomethacin on male fertility. While its therapeutic benefits are well established, its impact on male fertility has not been documented. The findings of this study suggest that men who use indomethacin regularly may be at a higher risk of experiencing negative impacts on their reproductive health, including reduced sperm quality.
吲哚美辛是一种非甾体抗炎药(NSAID),主要用于治疗疼痛、发烧、头痛和炎症。因此,本研究旨在评估吲哚美辛对男性生殖系统的影响,重点关注精子质量。将20只雄性大鼠分为4组(1、2、3、4组),1组为对照组。其余3组分别给予不同剂量的吲哚美辛(2 ~ 4组分别给予1mg/kg、2mg/kg和4mg/kg),疗程21 d。在这段时间后,从所有组中收集精子样本并进行分析以评估精子质量。分析的精子质量包括;精子活力、精液粘度、精子形态、精子数量等研究结果表明,吲哚美辛对大鼠精子质量有负面影响,降低了精子数量和精子活力,对精子整体质量产生不利影响。这项研究为吲哚美辛对男性生育能力的潜在副作用提供了重要的见解。虽然它的治疗效果是公认的,但它对男性生育能力的影响还没有文献记载。这项研究的结果表明,经常使用吲哚美辛的男性可能会对他们的生殖健康产生负面影响,包括精子质量下降。
{"title":"The Effect of Indomethacin on the Sperm Motility of Adult Wistar Rats","authors":"L. Zitte, Prisca I. Uzodinma","doi":"10.9734/ajob/2023/v17i4330","DOIUrl":"https://doi.org/10.9734/ajob/2023/v17i4330","url":null,"abstract":"Indomethacin is a non-steroidal anti-inflammatory drug (NSAID) used mostly in the treatment of pain, feverish condition, headaches and inflammation. This study was therefore carried out to evaluate the effect of indomethacin on the male reproductive system, focusing on sperm quality. Twenty male rats were divided into four groups, (groups1,2,3,and 4) with group one serving as control. The other three groups receiving different doses of indomethacin, (groups 2-4 receiving 1mg/kg, 2mg/kg and 4mg/kg respectively) for 21 days. After the period, sperm samples were collected from all the groups and analyzed to evaluate sperm quality. The sperm qualities analyzed include; sperm motility, semen viscosity, sperm morphology, sperm count, etc. the results of the study indicated that indomethacin had a negative impact on the sperm qualities of the rats, as it decreased sperm count and sperm motility , and had a detrimental effect on the overall sperm quality. This study provides important insights into the potential side effects of indomethacin on male fertility. While its therapeutic benefits are well established, its impact on male fertility has not been documented. The findings of this study suggest that men who use indomethacin regularly may be at a higher risk of experiencing negative impacts on their reproductive health, including reduced sperm quality.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74007279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Scent Leaf (Ocimum gratissimum) Aqueous Extract on Neuromuscular Functions of Albino Rats (Rattus norvegicus) 香叶水提物对褐家鼠神经肌肉功能的影响
Pub Date : 2023-04-11 DOI: 10.9734/ajob/2023/v17i4331
L. Zitte, Udoh, Matthew Edem
Ocimum gratissimum (O.G.) (scent leaf) has been used for the treatment of various disease situations from ancient time because of its anti-convulsion, anti-diabetic anti septic, antibacterial, anticancer, antioxidant properties. In the present study, the effects of O.G extract preparation was evaluated on the neuromuscular system of the albino Wister rat (Rattus norvegicus). Twenty five young animals of 40-90g were used for the experiment. They were divided into five groups of five animals per group. The different groups (groups 2, 3, 4, and 5) were administered with 100mg/kg, 200mg/kg, 400mg/kg, and 800mg/kg daily, respectively while group 1 was used as the control. The neuromuscular activities were assessed and studied by using scientific assays like the handgrip test, inverted screen test, swimming test, and the beam walking for checking non-declarative memory function. From the result obtained , One way analysis of variance (ANOVA) was used to compare the mean values among the different groups. Differences were considered significant whenever the p-value was P ≤ 0.05. From the different results evaluated it was observed that in handgrip test, groups 2, 3 and 5 with hanging time of 11.7±1.18, 13.55±1.37 and 11.5±0.52 respectively were significantly different ≤ 0.05 from the control with 6.6±1.13 second hanging time. Beam walking had a dose dependent slowness in walking across the beam among the treated groups as compared with the control. Inverted screen test has group 3 with the highest performance time of 20.55±8.42 which was significantly higher than the control with 8.4±1.98 seconds This result reveals that the administration of O.G. have significant effect on the cognitive, coordination and neuromuscular functions of the albino rats. It also shows a window of effectiveness of the extract on neuromuscular activities.
香叶因其抗惊厥、抗糖尿病、抗脓毒症、抗菌、抗癌、抗氧化等特性,自古以来就被用于治疗各种疾病。本研究以白化褐家鼠(Rattus norvegicus)为研究对象,探讨了黄芪提取物制剂对其神经肌肉系统的影响。实验选用40 ~ 90g幼龄动物25只。它们被分成五组,每组5只。2、3、4、5组分别给予100mg/kg、200mg/kg、400mg/kg、800mg/kg的日剂量,1组为对照组。采用握力测试、倒屏测试、游泳测试、横走测试等科学方法对神经肌肉活动进行评估和研究,检查非陈述性记忆功能。根据所得结果,采用单因素方差分析(One - way analysis of variance, ANOVA)比较各组间的平均值。当P值P≤0.05时,认为差异显著。从不同的评估结果来看,在握力测试中,悬挂时间分别为11.7±1.18、13.55±1.37和11.5±0.52的2、3和5组与悬挂时间为6.6±1.13秒的对照组相比差异≤0.05。与对照组相比,治疗组在光束上行走的速度与剂量有关。倒屏试验中,第3组表现时间最高,为20.55±8.42秒,显著高于对照组的8.4±1.98秒。结果表明,给药O.G.对白化大鼠的认知、协调和神经肌肉功能有显著影响。它还显示了提取物对神经肌肉活动的有效性。
{"title":"Effect of Scent Leaf (Ocimum gratissimum) Aqueous Extract on Neuromuscular Functions of Albino Rats (Rattus norvegicus)","authors":"L. Zitte, Udoh, Matthew Edem","doi":"10.9734/ajob/2023/v17i4331","DOIUrl":"https://doi.org/10.9734/ajob/2023/v17i4331","url":null,"abstract":"Ocimum gratissimum (O.G.) (scent leaf) has been used for the treatment of various disease situations from ancient time because of its anti-convulsion, anti-diabetic anti septic, antibacterial, anticancer, antioxidant properties. In the present study, the effects of O.G extract preparation was evaluated on the neuromuscular system of the albino Wister rat (Rattus norvegicus). Twenty five young animals of 40-90g were used for the experiment. They were divided into five groups of five animals per group. The different groups (groups 2, 3, 4, and 5) were administered with 100mg/kg, 200mg/kg, 400mg/kg, and 800mg/kg daily, respectively while group 1 was used as the control. The neuromuscular activities were assessed and studied by using scientific assays like the handgrip test, inverted screen test, swimming test, and the beam walking for checking non-declarative memory function. From the result obtained , One way analysis of variance (ANOVA) was used to compare the mean values among the different groups. Differences were considered significant whenever the p-value was P ≤ 0.05. From the different results evaluated it was observed that in handgrip test, groups 2, 3 and 5 with hanging time of 11.7±1.18, 13.55±1.37 and 11.5±0.52 respectively were significantly different ≤ 0.05 from the control with 6.6±1.13 second hanging time. Beam walking had a dose dependent slowness in walking across the beam among the treated groups as compared with the control. Inverted screen test has group 3 with the highest performance time of 20.55±8.42 which was significantly higher than the control with 8.4±1.98 seconds This result reveals that the administration of O.G. have significant effect on the cognitive, coordination and neuromuscular functions of the albino rats. It also shows a window of effectiveness of the extract on neuromuscular activities.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75785424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Asian Journal of Cell Biology
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