Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647683
Sang-hoon Choi, C. Oh
Flounder B lymphocytes isolated from different tissues were studied in terms of cell proliferation, apoptosis and the effects of cortisol on these processes. B lymphocytes, isolated from the flounder head kidney and spleen, were characterized by higher proliferation and lower intracellular calcium (Ca2*i) response to Ig‐crosslinking compared with peripheral blood B lymphocytes. Cortisol induced high levels of apoptosis (150% of control levels) in peripheral blood B lymphocytes, in combination with a stimulatory LPS signal. Head kidney and to a lesser extent spleen B lymphocytes, although less sensitive than their equivalent in peripheral blood, underwent cortisol‐induced apoptosis irrespective of extra stimulation up to 142% of control levels. Also proliferation with and without LPS stimulation was suppressed by cortisol (compared to plasma values measured during stress conditions) that is effective in inducing a significant increase in apoptosis in all three populations of B‐cells, suggesting that cortisol may be important for immunoregulation in both stressed and non‐stressed conditions. This implies possible severe impact of stress on lymphocyte development and activity. Different sensitivity of B‐cells to the corticosteroid, with respect to developmental stage and activity, may prevent excessive and long lasting depletion of B‐lymphocytes.
{"title":"The effect of cortisol on proliferative properties of flounder (Paralychthys Olivaceus) B Lymphocytes","authors":"Sang-hoon Choi, C. Oh","doi":"10.1080/12265071.2003.9647683","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647683","url":null,"abstract":"Flounder B lymphocytes isolated from different tissues were studied in terms of cell proliferation, apoptosis and the effects of cortisol on these processes. B lymphocytes, isolated from the flounder head kidney and spleen, were characterized by higher proliferation and lower intracellular calcium (Ca2*i) response to Ig‐crosslinking compared with peripheral blood B lymphocytes. Cortisol induced high levels of apoptosis (150% of control levels) in peripheral blood B lymphocytes, in combination with a stimulatory LPS signal. Head kidney and to a lesser extent spleen B lymphocytes, although less sensitive than their equivalent in peripheral blood, underwent cortisol‐induced apoptosis irrespective of extra stimulation up to 142% of control levels. Also proliferation with and without LPS stimulation was suppressed by cortisol (compared to plasma values measured during stress conditions) that is effective in inducing a significant increase in apoptosis in all three populations of B‐cells, suggesting that cortisol may be important for immunoregulation in both stressed and non‐stressed conditions. This implies possible severe impact of stress on lymphocyte development and activity. Different sensitivity of B‐cells to the corticosteroid, with respect to developmental stage and activity, may prevent excessive and long lasting depletion of B‐lymphocytes.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"57 - 62"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647683","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647695
Min-Jeong Kim, D. Chang, C. Lim, W. Park, B. Kaang
Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast‐based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.
{"title":"Screening of cDNAs encoding secreted and membrane proteins in the nervous system of marine snail Aplysia kurodai","authors":"Min-Jeong Kim, D. Chang, C. Lim, W. Park, B. Kaang","doi":"10.1080/12265071.2003.9647695","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647695","url":null,"abstract":"Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast‐based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"133 - 137"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647695","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647686
Hak Yong Kim
Highly purified high performance thin layer chromatography (HPTLC) fractions containing a putative calcium influx factor (CIF) were prepared from the Jurkat cells and Xenopus oocytes in which Ca2+ stores were depleted by thapsigargin treatment and from the yeast in which intracellular Ca2+ stores were also depleted by genetic means. Micro injection of the fractions has been shown to elicit Ca2+‐dependent currents in Xenopus oocytes. The nature of the membrane currents evoked by the putative CIF appeared to be carried by chloride ions since the current was blocked by the selective chloride channel blocker 1 mM niflumic acid and its reversal potential was about ‐24 mV. Injection of the calcium chelator 1,2‐bis(2‐aminophenoxy)ethane‐N, N,N’,N‘‐tetraacetic acid (BAPTA) eradicated the current activities, suggesting the current responses are entirely Ca2+‐dependent. Moreover, the currents were sensitive to the removal of extracellular calcium, indicating the dependence on calcium entry through the plasma membrane calcium entry channels. CIF activities were insensitive to protease, heat, and acid treatments and to Dische‐reaction whereas the activities were sensitive to nucleotide pyrophosphatase and hydrazynolysis. The fraction might have a sugar because it was sensitive to Molisch test and Seliwaniff's resorcinol reaction. From the above results, CIF as a small and stable molecule seems to have pyrimidine, pyrophosphate, and a sugar moiety.
{"title":"Biochemical characterization of a putative calcium influx factor as a diffusible messenger in Jurkat cells, Xenopus oocytes, and yeast","authors":"Hak Yong Kim","doi":"10.1080/12265071.2003.9647686","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647686","url":null,"abstract":"Highly purified high performance thin layer chromatography (HPTLC) fractions containing a putative calcium influx factor (CIF) were prepared from the Jurkat cells and Xenopus oocytes in which Ca2+ stores were depleted by thapsigargin treatment and from the yeast in which intracellular Ca2+ stores were also depleted by genetic means. Micro injection of the fractions has been shown to elicit Ca2+‐dependent currents in Xenopus oocytes. The nature of the membrane currents evoked by the putative CIF appeared to be carried by chloride ions since the current was blocked by the selective chloride channel blocker 1 mM niflumic acid and its reversal potential was about ‐24 mV. Injection of the calcium chelator 1,2‐bis(2‐aminophenoxy)ethane‐N, N,N’,N‘‐tetraacetic acid (BAPTA) eradicated the current activities, suggesting the current responses are entirely Ca2+‐dependent. Moreover, the currents were sensitive to the removal of extracellular calcium, indicating the dependence on calcium entry through the plasma membrane calcium entry channels. CIF activities were insensitive to protease, heat, and acid treatments and to Dische‐reaction whereas the activities were sensitive to nucleotide pyrophosphatase and hydrazynolysis. The fraction might have a sugar because it was sensitive to Molisch test and Seliwaniff's resorcinol reaction. From the above results, CIF as a small and stable molecule seems to have pyrimidine, pyrophosphate, and a sugar moiety.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"75 - 79"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647686","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647705
Hansol Lee, Hae‐Mi Yoo, Chang-Seok Lee
White snakeroot (Ageratina altissima (L.) R. King & H. Robinson) as an invasive alien plant appeared more abundantly at lower elevations where frequent artificial interferences prevailed than at higher elevations where such impacts were less. They appeared abundantly in introduced forests such as black locust plantation but they did not appear or were rare in natural forests such as oak forest. But an exceptional phenomenon where white snakeroot did not appear was found in a Korean pine stand with dense cover afforested recently. Appearance status of white snakeroot in each section of trampling path depended on breadth of the path and relative light intensity. Growth of white snakeroot measured as the number of ramet per genet, height, and biomass was better near the trampling path and was reduced toward the forest interior. The growth was proportionate to the relative light intensity measured according to distance from the trampling path. Such results support the fact generally known in relation to invasion and expansion of the invasive alien plants. From this viewpoint, we suggest a management plan that applies ecological restoration principles to address ecosystems infected with white snakeroot by restoring the integral feature of the degraded nature and more thoroughly conserving the remaining nature.
{"title":"Distribution pattern of white snakeroot as an invasive alien plant and restoration strategy to inhibit its expansion in Seoripool park, Seoul","authors":"Hansol Lee, Hae‐Mi Yoo, Chang-Seok Lee","doi":"10.1080/12265071.2003.9647705","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647705","url":null,"abstract":"White snakeroot (Ageratina altissima (L.) R. King & H. Robinson) as an invasive alien plant appeared more abundantly at lower elevations where frequent artificial interferences prevailed than at higher elevations where such impacts were less. They appeared abundantly in introduced forests such as black locust plantation but they did not appear or were rare in natural forests such as oak forest. But an exceptional phenomenon where white snakeroot did not appear was found in a Korean pine stand with dense cover afforested recently. Appearance status of white snakeroot in each section of trampling path depended on breadth of the path and relative light intensity. Growth of white snakeroot measured as the number of ramet per genet, height, and biomass was better near the trampling path and was reduced toward the forest interior. The growth was proportionate to the relative light intensity measured according to distance from the trampling path. Such results support the fact generally known in relation to invasion and expansion of the invasive alien plants. From this viewpoint, we suggest a management plan that applies ecological restoration principles to address ecosystems infected with white snakeroot by restoring the integral feature of the degraded nature and more thoroughly conserving the remaining nature.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"197 - 205"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647705","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647710
Y. Jung, Sungwon J. Hong
Recent evidence on behaviors in macaque monkeys indicate that the medial temporal cortical areas such as the entorhinal cortex (EC), perirhinal cortex, and parahippocampal cortex (PHC) are importantly involved in limbic and sensory memory function. Neuroanatomical studies also have demonstrated that the medial temporal cortical areas are connected with the ventral striatum, although comparatively little is known about the precise topography of these connections. We investigated the topographic organization of connections between the medial temporal cortical areas and the ventral striatum by placing retrograde tracers into five different regions of the ventral striatum: the ventromedial caudate nucleus, ventral shell, central shell, dorsal core of the nucleus accumbens (NA), and ventrolateral putamen. We found that the shell of the NA was the main projection site from the medial temporal cortical areas. Within the shell of the NA, there were also differential connections: EC diffusely innervates shell of the NA, while the projections from the perirhinal cortex and PHC concentrate on the ventral shell of the NA. Taken together, it is possible that the ventral shell of the NA is the main integration site of the limbic and sensory memory coming from the EC, perirhinal cortex, and PHC.
{"title":"Organization of projections from the medial temporal cortical areas to the ventral striatum in macaque monkeys","authors":"Y. Jung, Sungwon J. Hong","doi":"10.1080/12265071.2003.9647710","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647710","url":null,"abstract":"Recent evidence on behaviors in macaque monkeys indicate that the medial temporal cortical areas such as the entorhinal cortex (EC), perirhinal cortex, and parahippocampal cortex (PHC) are importantly involved in limbic and sensory memory function. Neuroanatomical studies also have demonstrated that the medial temporal cortical areas are connected with the ventral striatum, although comparatively little is known about the precise topography of these connections. We investigated the topographic organization of connections between the medial temporal cortical areas and the ventral striatum by placing retrograde tracers into five different regions of the ventral striatum: the ventromedial caudate nucleus, ventral shell, central shell, dorsal core of the nucleus accumbens (NA), and ventrolateral putamen. We found that the shell of the NA was the main projection site from the medial temporal cortical areas. Within the shell of the NA, there were also differential connections: EC diffusely innervates shell of the NA, while the projections from the perirhinal cortex and PHC concentrate on the ventral shell of the NA. Taken together, it is possible that the ventral shell of the NA is the main integration site of the limbic and sensory memory coming from the EC, perirhinal cortex, and PHC.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"237 - 248"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647710","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647723
Hye-Jeong Park, Hyeon-cheol Cha
We analyzed and compared profiles of flavonols extracted from leaves and exocarps of the grape Kyoho by TLC, HPLC and UV spectrophotometry. In the exocarps, quercetin 3‐O‐glucoside was the main compound while isorhamnetin 3‐O‐glycoside (I) was present in minor amounts. In leaves, on the other hand, quercetin 3‐O‐glucoside and quercetin 3‐O‐glucoside‐7‐O‐glucronide were the major compounds while isorhamnetin 3‐O‐glycoside (II) and kaempferol 3, 7‐O‐diglycoside were present in minor amounts.
{"title":"Flavonoids from leaves and exocarps of the grape Kyoho","authors":"Hye-Jeong Park, Hyeon-cheol Cha","doi":"10.1080/12265071.2003.9647723","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647723","url":null,"abstract":"We analyzed and compared profiles of flavonols extracted from leaves and exocarps of the grape Kyoho by TLC, HPLC and UV spectrophotometry. In the exocarps, quercetin 3‐O‐glucoside was the main compound while isorhamnetin 3‐O‐glycoside (I) was present in minor amounts. In leaves, on the other hand, quercetin 3‐O‐glucoside and quercetin 3‐O‐glucoside‐7‐O‐glucronide were the major compounds while isorhamnetin 3‐O‐glycoside (II) and kaempferol 3, 7‐O‐diglycoside were present in minor amounts.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"327 - 330"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647723","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647701
T. Angeline, K. Ramadevi, R. Aruna, G. Mohan, N. Jeyaraj
Sodium, potassium, calcium, zinc and magnesium levels in the serum of 31 patients diagnosed as acute myocardial infarction were analyzed on admission (within 24 Hours) and after 48 hours. The results were compared with those of 26 age matched controls. No significant difference was observed in the mean sodium, potassium, calcium and zinc levels between the cases and controls. Compared to the controls, however, the variation in the level of magnesium is highly significant at the time of admission as well as after 48 hours. When the risk factors like diabetes mellitus, hypertension, smoking and alcohol were considered, it is found that there is no significant difference between the risk groups as well as between the patients. The alteration in magnesium level in acute myocardial infarction is independent of these risk factors. Within the first 24 hours, the significant decrease in serum magnesium (35–51% fall when compared with the control group), correlates with its entry into the cell following ischemia. From this hypo‐magnesemic state, it rises to 9–22 times after 48 hours. This hyper‐magnesemia after 48 hours is probably due to the shift of magnesuim from the intracellular fluid compartment to the extracellular fluid compartment that follows cellular recovery. Therefore, including magnesium in the immediate management of acute myocardial infarction will be beneficial in the early recovery.
{"title":"Alteration in magnesium level in acute myocardial infarction","authors":"T. Angeline, K. Ramadevi, R. Aruna, G. Mohan, N. Jeyaraj","doi":"10.1080/12265071.2003.9647701","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647701","url":null,"abstract":"Sodium, potassium, calcium, zinc and magnesium levels in the serum of 31 patients diagnosed as acute myocardial infarction were analyzed on admission (within 24 Hours) and after 48 hours. The results were compared with those of 26 age matched controls. No significant difference was observed in the mean sodium, potassium, calcium and zinc levels between the cases and controls. Compared to the controls, however, the variation in the level of magnesium is highly significant at the time of admission as well as after 48 hours. When the risk factors like diabetes mellitus, hypertension, smoking and alcohol were considered, it is found that there is no significant difference between the risk groups as well as between the patients. The alteration in magnesium level in acute myocardial infarction is independent of these risk factors. Within the first 24 hours, the significant decrease in serum magnesium (35–51% fall when compared with the control group), correlates with its entry into the cell following ischemia. From this hypo‐magnesemic state, it rises to 9–22 times after 48 hours. This hyper‐magnesemia after 48 hours is probably due to the shift of magnesuim from the intracellular fluid compartment to the extracellular fluid compartment that follows cellular recovery. Therefore, including magnesium in the immediate management of acute myocardial infarction will be beneficial in the early recovery.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"169 - 171"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647701","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647702
Ronojoy Sen Gupta, J. Ahnn
The transition metal cadmium is a serious occupational and environmental toxin. To inhibit cadmium‐induced damage, cells respond by increasing the expression of genes that encode stress‐responsive proteins. The metal‐regulatory transcription factor 1 (MTF‐1) is a key regulator of heavy‐metal induced transcription of metallothionein‐I and II and other genes in mammals and other metazoans. Transcriptional activation of genes by MTF‐1 is mediated through binding to metal‐responsive elements in the target gene promoters. Phosphorylation of MTF‐1 plays a critical role in the cadmium‐inducible transcriptional activation of metallothionein and other responses. Studies using inhibitors indicate that multiple kinases and signal transduction cascades, including those mediated by protein kinase C, tyrosine kinase and casein kinase II, are essential for cadmium‐mediated transcriptional activation. In addition, calcium signaling is also involved in regulating metal‐activated transcription. In several species, cadmium induces heat shock genes. Recently much progress has been made in elucidating the cellular machinery that regulates this metal‐inducible gene expression. This review summarizes these recent advances in understanding the role of some known cadmium‐responsive genes and the molecular mechanisms that activate metal‐responsive transcription factor, MTF‐1.
{"title":"Cadmium‐induced gene expression is regulated by MTF‐1, a key metal‐responsive transcription factor","authors":"Ronojoy Sen Gupta, J. Ahnn","doi":"10.1080/12265071.2003.9647702","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647702","url":null,"abstract":"The transition metal cadmium is a serious occupational and environmental toxin. To inhibit cadmium‐induced damage, cells respond by increasing the expression of genes that encode stress‐responsive proteins. The metal‐regulatory transcription factor 1 (MTF‐1) is a key regulator of heavy‐metal induced transcription of metallothionein‐I and II and other genes in mammals and other metazoans. Transcriptional activation of genes by MTF‐1 is mediated through binding to metal‐responsive elements in the target gene promoters. Phosphorylation of MTF‐1 plays a critical role in the cadmium‐inducible transcriptional activation of metallothionein and other responses. Studies using inhibitors indicate that multiple kinases and signal transduction cascades, including those mediated by protein kinase C, tyrosine kinase and casein kinase II, are essential for cadmium‐mediated transcriptional activation. In addition, calcium signaling is also involved in regulating metal‐activated transcription. In several species, cadmium induces heat shock genes. Recently much progress has been made in elucidating the cellular machinery that regulates this metal‐inducible gene expression. This review summarizes these recent advances in understanding the role of some known cadmium‐responsive genes and the molecular mechanisms that activate metal‐responsive transcription factor, MTF‐1.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"173 - 186"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59656008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647684
J. H. Kim, Young Ju Lee, Juyeon Nam, H. Shim, S. Choe
Until recently, interest in human complement receptor type I (CR1) has focused on immune complex processing, which contributed to our understanding of regulatory mechanism of complement activation. However, the promoter structure and transcriptional regulation of human CR1 gene has not been clear. To study the unique regulation of human CR1 gene expression, we assessed promoter activity of the 5'‐flanking region of human CR1 gene using transient transfection and gel mobility shift assays. In this study we demonstrated that NF‐Y binds to the inverted CCAAT element and that the functional interaction with protein(s) which bind to the GC‐rich motif may be necessary for optimal transcription of human CR1 gene. We also show that sequence elements which located at ‐95/‐58 and +45/ +50 are important for optimal transcription of CR1 gene.
{"title":"Promoter structure and transcriptional activity of human complement receptor type I (CR1) gene","authors":"J. H. Kim, Young Ju Lee, Juyeon Nam, H. Shim, S. Choe","doi":"10.1080/12265071.2003.9647684","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647684","url":null,"abstract":"Until recently, interest in human complement receptor type I (CR1) has focused on immune complex processing, which contributed to our understanding of regulatory mechanism of complement activation. However, the promoter structure and transcriptional regulation of human CR1 gene has not been clear. To study the unique regulation of human CR1 gene expression, we assessed promoter activity of the 5'‐flanking region of human CR1 gene using transient transfection and gel mobility shift assays. In this study we demonstrated that NF‐Y binds to the inverted CCAAT element and that the functional interaction with protein(s) which bind to the GC‐rich motif may be necessary for optimal transcription of human CR1 gene. We also show that sequence elements which located at ‐95/‐58 and +45/ +50 are important for optimal transcription of CR1 gene.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"63 - 68"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647684","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647690
Y. Park, J. Choe
Morphological characteristics of nymphal instars are described for a Korean wood‐feeding cockroach, Cryptocercus kyebangensis. Eleven instars, including adults, were tentatively identified. Female adults had apicolateral emargination and a truncated apical median prominence in the seventh stemite, and female nymphs of the other instars except for the last had a narrowly rounded apical median prominence in the seventh stemite. In the last instar, the seventh stemite was partially desclerotized and somewhat shriveled at the start of the emarginated area. In contrast, males showed no emargination in adults, and had a rounded broad apical median in this area of all instars. In the ninth stemite, female nymphs had a medium notch on the caudal margin and styli were separated before reaching adulthood, whereas no such notch was observed in male nymphs. The styli remained prominent in the ninth stemite of male adults.
{"title":"Morphological differences of immature stages between males and females in a Korean wood‐feeding cockroach (cryptocercus kyebangensis)","authors":"Y. Park, J. Choe","doi":"10.1080/12265071.2003.9647690","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647690","url":null,"abstract":"Morphological characteristics of nymphal instars are described for a Korean wood‐feeding cockroach, Cryptocercus kyebangensis. Eleven instars, including adults, were tentatively identified. Female adults had apicolateral emargination and a truncated apical median prominence in the seventh stemite, and female nymphs of the other instars except for the last had a narrowly rounded apical median prominence in the seventh stemite. In the last instar, the seventh stemite was partially desclerotized and somewhat shriveled at the start of the emarginated area. In contrast, males showed no emargination in adults, and had a rounded broad apical median in this area of all instars. In the ninth stemite, female nymphs had a medium notch on the caudal margin and styli were separated before reaching adulthood, whereas no such notch was observed in male nymphs. The styli remained prominent in the ninth stemite of male adults.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"105 - 109"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647690","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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