Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647698
C. Jeon, Jin-Young Sung, S. Hong
Calretinin is thought to play roles in calcium buffering. Its site of expression has been extensively studied in the central nervous system. We previously reported (Hong et al., 2002, Neurosci. Res., 44:325–335) calretinin expression in the superficial layers of the cat superior colliculus (SC). In the present study, we studied the distribution of calretinin in the intermediate and deep layers by immunocytochemistry. We found striking differences in calretinin immunoreactivity among the superficial, intermediate, and deep layers. In contrast to the superficial layers, the intermediate and deep layers contained many calretinin‐immunoreactive (IR) neurons. They formed two laminar tiers. The first tier, which was very distinctive, was found within the upper intermediate gray layers and formed clusters of labeled neurons in many sections. The second tier of calretinin‐IR neurons was found in the deep gray layer. However, the second tier was not distinctive compared to the first tier and the labeled neurons did not form any clusters. Calretinin‐IR neurons in the intermediate and deep layers varied dramatically in morphology and included vertical fusiform, pyriform, and stellate neurons. Neurons with varicose dendrites were also labeled. Most of the labeled neurons were small to medium in size. Enucleation appeared to have no effect on the distribution of calretinin immunoreactivity in the contralateral intermediate and deep layers of the SC. The results indicate that calretinin is present in various neurons, at different locations. These results should be applicable for better understanding of the functional organization of the SC.
{"title":"Distribution and morphology of calretinin‐immunoreactive neurons in the intermediate and deep layers of cat superior colliculus","authors":"C. Jeon, Jin-Young Sung, S. Hong","doi":"10.1080/12265071.2003.9647698","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647698","url":null,"abstract":"Calretinin is thought to play roles in calcium buffering. Its site of expression has been extensively studied in the central nervous system. We previously reported (Hong et al., 2002, Neurosci. Res., 44:325–335) calretinin expression in the superficial layers of the cat superior colliculus (SC). In the present study, we studied the distribution of calretinin in the intermediate and deep layers by immunocytochemistry. We found striking differences in calretinin immunoreactivity among the superficial, intermediate, and deep layers. In contrast to the superficial layers, the intermediate and deep layers contained many calretinin‐immunoreactive (IR) neurons. They formed two laminar tiers. The first tier, which was very distinctive, was found within the upper intermediate gray layers and formed clusters of labeled neurons in many sections. The second tier of calretinin‐IR neurons was found in the deep gray layer. However, the second tier was not distinctive compared to the first tier and the labeled neurons did not form any clusters. Calretinin‐IR neurons in the intermediate and deep layers varied dramatically in morphology and included vertical fusiform, pyriform, and stellate neurons. Neurons with varicose dendrites were also labeled. Most of the labeled neurons were small to medium in size. Enucleation appeared to have no effect on the distribution of calretinin immunoreactivity in the contralateral intermediate and deep layers of the SC. The results indicate that calretinin is present in various neurons, at different locations. These results should be applicable for better understanding of the functional organization of the SC.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"151 - 157"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647721
H. Yoon, Y. H. Kim, Sung Wook Park, Hwanghee-Blaise Lee, H. Park
Renal dipeptidase (RDPase, membrane dipeptidase, dehydropeptidase 1, EC 3.4.13.19) has been widely studied since it was first purified from porcine kidney brush border membrane. It was reported that RDPase activity in urine samples of acute and chronic renal failure patients decreases. Nitric oxide (NO) is a highly reactive free radical involved in a number of physiological and pathological processes. NO is able to act in a dual mode, leading either to induction of apoptosis or to blunted execution of programmed cell death. NO inhibited the RDPase release from porcine renal proximal tubules, which could be blocked by L‐NAME. Chitosan, the linear polymer of D‐glucosamine in β(1?4) linkage, not only reversed the decreased RDPase release by NO but also increased NO production in the proximal tubule cells. The stimulatory effect of NO on RDPase release from proximal tubules in the presence of chitosan must be different from the previously proposed mechanism of RDPase release via NO signaling pathway. Chitosan stimulated the RDPase release in the proximal tubules and increased RDPase activity to 220% and 250% at 0.1% and 1%, respectively. RDPase release was decreased to about 40% in the injured proximal tubules and was recovered in proportion to the increase of chitosan. Chitosan may be useful in recovery of renal function from HgCl2 injury.
{"title":"Chitosan increases the release of renal dipeptidase from porcine renal proximal tubule cells","authors":"H. Yoon, Y. H. Kim, Sung Wook Park, Hwanghee-Blaise Lee, H. Park","doi":"10.1080/12265071.2003.9647721","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647721","url":null,"abstract":"Renal dipeptidase (RDPase, membrane dipeptidase, dehydropeptidase 1, EC 3.4.13.19) has been widely studied since it was first purified from porcine kidney brush border membrane. It was reported that RDPase activity in urine samples of acute and chronic renal failure patients decreases. Nitric oxide (NO) is a highly reactive free radical involved in a number of physiological and pathological processes. NO is able to act in a dual mode, leading either to induction of apoptosis or to blunted execution of programmed cell death. NO inhibited the RDPase release from porcine renal proximal tubules, which could be blocked by L‐NAME. Chitosan, the linear polymer of D‐glucosamine in β(1?4) linkage, not only reversed the decreased RDPase release by NO but also increased NO production in the proximal tubule cells. The stimulatory effect of NO on RDPase release from proximal tubules in the presence of chitosan must be different from the previously proposed mechanism of RDPase release via NO signaling pathway. Chitosan stimulated the RDPase release in the proximal tubules and increased RDPase activity to 220% and 250% at 0.1% and 1%, respectively. RDPase release was decreased to about 40% in the injured proximal tubules and was recovered in proportion to the increase of chitosan. Chitosan may be useful in recovery of renal function from HgCl2 injury.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"309 - 315"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647721","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647726
A. Kim, Meoung‐Sook Lee, E. Park
The effects of acute acid stress on hatching success and hatching period of laboratory‐reared hermaphroditic fish Rivulus marmoratus were examined. The effects of acute acid toxicity on mortality was also determined in three life stages of this fish. There was a significant negative effect of acid stress on hatching performance in the R. marmoratus embryos. The hatching success was only 5% at pH 3.5 compared to over 78% at pH higher than 4.0. The hatching period was also delayed by low pH treatments. The larval and juvenile stages were more sensitive to acid toxicity on mortality than the adult stage, but larvae and juveniles showed similar sensitivity. The 96‐h LC50 value was pH 3.8 in larval and juvenile stages and pH 3.3 in adult stage.
{"title":"Effects of Acute Acid Stress on Hatching and Mortality of Hermaphroditic Teleost, Rivulus marmoratus (Cyprinodontiformes; Rivulidae)","authors":"A. Kim, Meoung‐Sook Lee, E. Park","doi":"10.1080/12265071.2003.9647726","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647726","url":null,"abstract":"The effects of acute acid stress on hatching success and hatching period of laboratory‐reared hermaphroditic fish Rivulus marmoratus were examined. The effects of acute acid toxicity on mortality was also determined in three life stages of this fish. There was a significant negative effect of acid stress on hatching performance in the R. marmoratus embryos. The hatching success was only 5% at pH 3.5 compared to over 78% at pH higher than 4.0. The hatching period was also delayed by low pH treatments. The larval and juvenile stages were more sensitive to acid toxicity on mortality than the adult stage, but larvae and juveniles showed similar sensitivity. The 96‐h LC50 value was pH 3.8 in larval and juvenile stages and pH 3.3 in adult stage.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"345 - 348"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647726","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647703
C. Sim, K. Lee
A new sponge species of the genus Hymeniacidon (family Halichondriidae) from Munsum, Jeju Island, Korea is described. This species is similar to H. sinapium in spicule size, but the color, oscules, external surface and arrangement of the spicules are considerably different. This species is bright yellow in live, and retains multiple oscules and a plumose bundle of styles in the subectosomal region.
{"title":"A new species of the genus hymeniacidon (Demospongiae: Halichondrida: Halichondriidae) from Korea","authors":"C. Sim, K. Lee","doi":"10.1080/12265071.2003.9647703","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647703","url":null,"abstract":"A new sponge species of the genus Hymeniacidon (family Halichondriidae) from Munsum, Jeju Island, Korea is described. This species is similar to H. sinapium in spicule size, but the color, oscules, external surface and arrangement of the spicules are considerably different. This species is bright yellow in live, and retains multiple oscules and a plumose bundle of styles in the subectosomal region.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"187 - 189"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647703","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59656021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647687
Yong Sun Lee, June-Chul Lee, H. Kim, Sukhyun Kang, Joo Seok Han, J. B. Kim, D. Hwang
While screening proteins that interact with DnaA protein, the initiator protein for Escherichia coli chromosomal DNA replication, we found a 52‐kD sized protein which bound to DnaA protein in a salt‐dependent manner. This protein was identified as trigger factor, a ribosome‐associated peptidyl‐prolyl‐cis/trans isomerase with chaperone activity. Trigger factor was overproduced and purified to near homogeneity, and its effect on the function of DnaA protein was examined. Enhanced binding of DnaA protein to DnaA box with no apparent super shift in the gel‐shift experiments suggested that trigger factor, by virtue of its chaperone activity, exerts a change on DnaA protein thus increasing its binding affinity for DnaA box.
{"title":"Trigger factor interacts with DnaA protein to stimulate its interaction with DnaA box","authors":"Yong Sun Lee, June-Chul Lee, H. Kim, Sukhyun Kang, Joo Seok Han, J. B. Kim, D. Hwang","doi":"10.1080/12265071.2003.9647687","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647687","url":null,"abstract":"While screening proteins that interact with DnaA protein, the initiator protein for Escherichia coli chromosomal DNA replication, we found a 52‐kD sized protein which bound to DnaA protein in a salt‐dependent manner. This protein was identified as trigger factor, a ribosome‐associated peptidyl‐prolyl‐cis/trans isomerase with chaperone activity. Trigger factor was overproduced and purified to near homogeneity, and its effect on the function of DnaA protein was examined. Enhanced binding of DnaA protein to DnaA box with no apparent super shift in the gel‐shift experiments suggested that trigger factor, by virtue of its chaperone activity, exerts a change on DnaA protein thus increasing its binding affinity for DnaA box.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"81 - 87"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647687","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647691
Y. Park, J. Choe
Ecological aspects of Cryptocercus kyebangensis life history were investigated via laboratory rearing and field observations. The number of antennal segments and head width were used to classify the first four instars. The results, which combine both the field collection and the laboratory rearing, indicate that eleven instars occur in C. kyebangensis. It supports the proposal on the number of instars of Park and Choe (2003c) based on field collections. A total of 388 nymphs from 13 colonies were collected prior to winter to investigate overwintering stages. Of them, 4% (n = 17) were the second instars, 57% (n = 220) were the third instars, and 39% (n = 151) were the fourth instars, respectively. Thus, most of them overwinter in the third or fourth instars. The results indicate that young nymphs of C. kyebangensis have to reach at least 3rd or 4th instar to survive low temperature environment of winter. According to seasonal dynamics of populations, C. kyebangensis reaches adulthood in the summer of the fourth or fifth year (4–5 yr span) after their birth.
{"title":"Life history and population dynamics of Korean woodroach (Cryptocercus kyebangensis) populations","authors":"Y. Park, J. Choe","doi":"10.1080/12265071.2003.9647691","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647691","url":null,"abstract":"Ecological aspects of Cryptocercus kyebangensis life history were investigated via laboratory rearing and field observations. The number of antennal segments and head width were used to classify the first four instars. The results, which combine both the field collection and the laboratory rearing, indicate that eleven instars occur in C. kyebangensis. It supports the proposal on the number of instars of Park and Choe (2003c) based on field collections. A total of 388 nymphs from 13 colonies were collected prior to winter to investigate overwintering stages. Of them, 4% (n = 17) were the second instars, 57% (n = 220) were the third instars, and 39% (n = 151) were the fourth instars, respectively. Thus, most of them overwinter in the third or fourth instars. The results indicate that young nymphs of C. kyebangensis have to reach at least 3rd or 4th instar to survive low temperature environment of winter. According to seasonal dynamics of populations, C. kyebangensis reaches adulthood in the summer of the fourth or fifth year (4–5 yr span) after their birth.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"111 - 117"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647691","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647692
J. Rho, J. Choe
In order to determine whether foals of more dominant mothers were harassed less by mares than those of lower‐ranked mothers, harassment of foals was studied in a herd of Jeju ponies (Equus caballus) consisting of 73 mares, their 53 foals, and one stallion. Mares harassed the foals on 71 occasions between March to September, 1998. The frequency of harassment tended to decrease as foals aged, but the relationship was not significant (P= 0.079). Foals were harassed when sleeping (n = 14), grazing (n = 19), moving (n = 8), approaching a mare in the vicinity (n = 13), or play‐fighting with the foal of the harassing mare (n = 17). Mares harassed foals of younger, more subordinate mares than those of older, more dominant mares (P< 0.001). We conclude that early social experience of foals is associated with the dominance rank of its mother.
{"title":"Difference of early experience of foals according to dominance ranks of their mothers in the Jeju pony (Equus caballus)","authors":"J. Rho, J. Choe","doi":"10.1080/12265071.2003.9647692","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647692","url":null,"abstract":"In order to determine whether foals of more dominant mothers were harassed less by mares than those of lower‐ranked mothers, harassment of foals was studied in a herd of Jeju ponies (Equus caballus) consisting of 73 mares, their 53 foals, and one stallion. Mares harassed the foals on 71 occasions between March to September, 1998. The frequency of harassment tended to decrease as foals aged, but the relationship was not significant (P= 0.079). Foals were harassed when sleeping (n = 14), grazing (n = 19), moving (n = 8), approaching a mare in the vicinity (n = 13), or play‐fighting with the foal of the harassing mare (n = 17). Mares harassed foals of younger, more subordinate mares than those of older, more dominant mares (P< 0.001). We conclude that early social experience of foals is associated with the dominance rank of its mother.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"119 - 122"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647692","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647706
Jongmin Kim, Jaeyoung Shin, Heonyong Park
Caveolin‐1 is a principal protein in the plasma membrane microdomains called caveolae. Caveolae play an important role in the transcytosis and pinocytosis. Therefore, caveolin‐1 is most likely to work for the membrane dynamic events. In addition, caveolin‐1 interacts with various signaling molecules. Although caveolin‐1 possesses a variety of physiological functions, its structural properties were little construed. Here we analyzed the structural dynamics of the N‐terminal caveolin‐1 (residues 1–101), in order to better understand the structural properties in terms of its versatile functionality. We first analyzed its oligomeric form using GST‐fused N‐terminal domain, revealing that it equilibrates between a dimer and monomers in a concentration‐dependent manner. The N‐terminal domain of caveolin‐1 was previously found to form a heptamer, so that our data suggest the dimeric form as an intermediate structure for the heptamer formation. Then, we obtained the folding profile, which indicated that AGH2o is about 0.5 ±0.03 kcal/mol. The stability of N‐terminal domain is relatively low, indicating that N‐terminal domain may not be crystalline. Conclusively, the dynamic and flexible structure of N‐terminal domain appears more favorable to maintain the versatile functions of caveolin‐1.
{"title":"Structural characterization for N‐terminal domain of caveolin‐1","authors":"Jongmin Kim, Jaeyoung Shin, Heonyong Park","doi":"10.1080/12265071.2003.9647706","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647706","url":null,"abstract":"Caveolin‐1 is a principal protein in the plasma membrane microdomains called caveolae. Caveolae play an important role in the transcytosis and pinocytosis. Therefore, caveolin‐1 is most likely to work for the membrane dynamic events. In addition, caveolin‐1 interacts with various signaling molecules. Although caveolin‐1 possesses a variety of physiological functions, its structural properties were little construed. Here we analyzed the structural dynamics of the N‐terminal caveolin‐1 (residues 1–101), in order to better understand the structural properties in terms of its versatile functionality. We first analyzed its oligomeric form using GST‐fused N‐terminal domain, revealing that it equilibrates between a dimer and monomers in a concentration‐dependent manner. The N‐terminal domain of caveolin‐1 was previously found to form a heptamer, so that our data suggest the dimeric form as an intermediate structure for the heptamer formation. Then, we obtained the folding profile, which indicated that AGH2o is about 0.5 ±0.03 kcal/mol. The stability of N‐terminal domain is relatively low, indicating that N‐terminal domain may not be crystalline. Conclusively, the dynamic and flexible structure of N‐terminal domain appears more favorable to maintain the versatile functions of caveolin‐1.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"207 - 211"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647706","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647715
Jae‐Chang Jung
Matrix metalloproteinases (MMPs) have been implicated in corneal diseases of the eye. Among the MMPs, collagenase‐1 (MMP‐1) participates in remodeling the extracellular matrix of corneal stroma during wound healing. Here the temporal expression patterns of both IL‐1α and collagenase‐1 were examined during regenerative corneal wound healing. IL‐1α was highly expressed in the healing epithelium at 3 d after epithelial abrasion wound, thereafter its expression returned to normal level. Primary cultured quiescent stromal cells (keratocytes) from uninjured normal cornea were round‐shaped and did not expresse collagenase‐1. However, freshly‐cultured stromal cells isolated 3d after epithelial abrasion wound (abrasion wound) underwent marked morphological changes, and a certain percentage of cell populations was transformed into fibroblasts. By immunofluorescence, collagenase‐1 expression was detected only in spindle‐shaped fibroblasts. Interestingly, most freshly‐isolated cells from remodeling corneal repair tissue 10 d after abrasion injury were found to have fibroblastic morphology, and also constitutively expressed a high level of collagenase‐1 in vitro primary culture. However, its expression was not found in the cells isolated 14 d after wound and cells seemed to have returned to normal quiescent stromal cells. Taken together, these results suggest that differentially expressed IL‐1α in the epithelium might play important roles in wound healing, and important signalling pathways are altered as cells take on the fibroblastic morphology.
{"title":"Cellular changes of phenotype and collagenase‐1 expression in healing corneal stromal cells","authors":"Jae‐Chang Jung","doi":"10.1080/12265071.2003.9647715","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647715","url":null,"abstract":"Matrix metalloproteinases (MMPs) have been implicated in corneal diseases of the eye. Among the MMPs, collagenase‐1 (MMP‐1) participates in remodeling the extracellular matrix of corneal stroma during wound healing. Here the temporal expression patterns of both IL‐1α and collagenase‐1 were examined during regenerative corneal wound healing. IL‐1α was highly expressed in the healing epithelium at 3 d after epithelial abrasion wound, thereafter its expression returned to normal level. Primary cultured quiescent stromal cells (keratocytes) from uninjured normal cornea were round‐shaped and did not expresse collagenase‐1. However, freshly‐cultured stromal cells isolated 3d after epithelial abrasion wound (abrasion wound) underwent marked morphological changes, and a certain percentage of cell populations was transformed into fibroblasts. By immunofluorescence, collagenase‐1 expression was detected only in spindle‐shaped fibroblasts. Interestingly, most freshly‐isolated cells from remodeling corneal repair tissue 10 d after abrasion injury were found to have fibroblastic morphology, and also constitutively expressed a high level of collagenase‐1 in vitro primary culture. However, its expression was not found in the cells isolated 14 d after wound and cells seemed to have returned to normal quiescent stromal cells. Taken together, these results suggest that differentially expressed IL‐1α in the epithelium might play important roles in wound healing, and important signalling pathways are altered as cells take on the fibroblastic morphology.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"271 - 277"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647715","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1080/12265071.2003.9647699
I. Choi
The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. The RAD3 encoded protein possesses a single stranded DNA‐dependent ATPase and DNA and DNA‐RNA helicase activities. To examine the extent of conservation of structure and function of a S. pombe RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (homolog of RAD3 gene). Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as HRD3 gene and this gene exists as a single copy in S. pombe. The transcript of 2. 8 kb was detected by Northern blot analysis. The level of transcripts increased by ultraviolet (UV) irradiation, indicating that HRD3 is one of the UV‐inducible genes in S. pombe. Furthermore, the predicted partial sequence of HRD3 protein has 60% identity to S. cerevisiae RAD3 gene. This homology was particularly striking in the regions identified as being conserved in a group of DNA helicases. Gene deletion experiments indicate that the HRD3 gene is essential for viability and DNA repair function. These observations suggest evolutionary conservation of other protein components with which HRD3 might interact in mediating its DNA repair and viability functions.
酿酒酵母的RAD3基因是切除修复所必需的，也是细胞生存所必需的。RAD3编码的蛋白具有单链DNA依赖的atp酶以及DNA和DNA - RNA解旋酶活性。为了研究pombe S. RAD3在真核生物进化过程中结构和功能的保守程度，通过筛选基因组DNA文库分离出RAD3同源基因。分离得到的基因被命名为HRD3(与RAD3基因同源)。Southern blot分析证实，pombe S. pombe染色体含有与HRD3基因相同的DNA，该基因在pombe S. pombe中以单拷贝的形式存在。[译文]Northern blot检测到8kb。在紫外线照射下，转录本水平升高，表明HRD3是紫外光诱导基因之一。预测的HRD3蛋白部分序列与酿酒酵母RAD3基因的同源性为60%。这种同源性在一组DNA解旋酶的保守区域尤为显著。基因缺失实验表明，HRD3基因对生存能力和DNA修复功能至关重要。这些观察结果表明，HRD3可能与其他蛋白质成分相互作用，介导其DNA修复和生存功能的进化守恒。
{"title":"Characterization of HRD3, a Schizosaccharomyces pombe gene involved in DNA repair and cell viability","authors":"I. Choi","doi":"10.1080/12265071.2003.9647699","DOIUrl":"https://doi.org/10.1080/12265071.2003.9647699","url":null,"abstract":"The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. The RAD3 encoded protein possesses a single stranded DNA‐dependent ATPase and DNA and DNA‐RNA helicase activities. To examine the extent of conservation of structure and function of a S. pombe RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (homolog of RAD3 gene). Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as HRD3 gene and this gene exists as a single copy in S. pombe. The transcript of 2. 8 kb was detected by Northern blot analysis. The level of transcripts increased by ultraviolet (UV) irradiation, indicating that HRD3 is one of the UV‐inducible genes in S. pombe. Furthermore, the predicted partial sequence of HRD3 protein has 60% identity to S. cerevisiae RAD3 gene. This homology was particularly striking in the regions identified as being conserved in a group of DNA helicases. Gene deletion experiments indicate that the HRD3 gene is essential for viability and DNA repair function. These observations suggest evolutionary conservation of other protein components with which HRD3 might interact in mediating its DNA repair and viability functions.","PeriodicalId":85060,"journal":{"name":"Korean journal of biological sciences","volume":"7 1","pages":"159 - 164"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/12265071.2003.9647699","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59655164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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