Behavioural Processes最新文献

The study of animal behaviour sometimes requires unique identification of individuals, especially in the study of social behaviours involving the interactions of multiple individuals. To this end, researchers have developed many different methods of marking individuals. For small animals like insects, paint marks are often applied to their bodies by anaesthetizing them using low temperature or carbon dioxide_. Despite this procedure being ubiquitous when studying social insects, the effect of paint and anaesthetics on their behaviour has not been well investigated, especially their effect on performance during a collective task. In our study, we investigate how paint marks and anaesthetics affect the movement and recruitment behaviours of the ant Temnothorax rugatulus in a house hunting context. We painted two thirds of colony members, half of them using CO₂ and the other half using low temperature as methods of anaesthetization, and left the one third unpainted as a control group. We then measured their exploratory behaviour prior to house hunting and their recruitment behaviours during house hunting. We found that neither paint marks nor anaesthetics reduce activity levels of these behaviours. However, low-temperature anaesthetized ants performed a higher number of recruitment behaviours than control ants. Because CO₂ anaesthetized ants performed all tasks at the same level as control ants, our data suggest that this is a good technique for paint marking ants, especially T. rugatulus. This is the first study empirically testing negative effects of paint marking on individual and collective outcomes in social insects. Our study represents an important step towards routine validation of individual identification methods used in the study of animal behaviour.

Increased reinforcer motivation in rats has been repeatedly demonstrated following intermittent-access (IntA) training, where the reinforcer is only available for brief periods during a session, compared to continuous-access (ContA) training where the reinforcer is available throughout the session. The present study investigated whether different associations learned during training on the two procedures contributes to the effect. Two experiments tested the importance of the stimulus-response (S-R) and stimulus-outcome (S-O) associations between the IntA availability cues and the training response and reinforcer, respectively. In Exp. 1, separate groups of rats were trained to lever press for saccharin on the IntA or ContA procedures. Increased motivation for saccharin was observed in the IntA group on a later progressive ratio test where nosepoking was the operant (but not when lever pressing was the operant). The outcome of the nosepoke test suggests that a potential S-R association formed during IntA training was not critical for the effect. In Exp. 2, increased saccharin motivation (on nosepoke tests) after IntA training (with lever pressing) was observed regardless of the presence or absence of IntA availability cues, indicating that the S-O association formed during training is not critical for the effect either. Overall, these results suggest that the elemental associations learned on IntA procedures may not be what drives increased motivation observed after IntA training.

Under certain conditions, pigeons prefer information about whether food will be forthcoming at the end of an interval to a higher chance of obtaining the food. In the typical protocol, choosing one option (Informative) is followed by one of two 10-s long terminal-link stimuli: S^G always ending in food or S^R never ending in food, with S^G occurring only 20% of the trials. The other option (Non-informative) is also followed by one of two 10-s long terminal-link stimuli: S^B or S^Y, both ending in food 50% of the trials. Although the Informative option yields food with a lower probability than the Non-informative (0.2 vs. 0.5), pigeons prefer it. To determine whether such preference occurs because S^G and S^R disambiguate the trial outcome immediately upon choice, we delayed the moment the disambiguation took place in two experiments. In Experiment 1, when the Informative option was chosen, S^G always ensued for t seconds of the terminal-link, and then the standard contingencies followed. Experiment 2 was similar, except that S^R always ensued for t seconds. Across conditions, t varied from 0 to 10 s. In both experiments, preference for the Informative option decreased with t, but the effect was stronger in Experiment 1. We discuss the implication of these findings for functional and mechanistic models of suboptimal choice.

Many animals regularly move between different locations within their home range. During these journeys, individuals are expected to use the shortest path, because this strategy minimizes energy expenditure and reduces exposure to adverse conditions, such as predation. The ability to find the shortest distance route has been demonstrated in ants, migrating birds and a few mammals. We investigated whether a freshwater fish, Danio rerio, exhibits this ability. Small groups of zebrafish were allowed to move between the two compartments of their tank using two paths differing in length. They developed a preference for the shorter path gradually over the six days of the experiment. Subjects’ accuracy in choosing the shorter path varied from below 60%, with a 20% length disparity, to 80% when one path was twice as long as the other. In a second experiment, zebrafish were initially allowed to practice in groups and then tested individually. We found evidence of individual and sex differences in performance, with males performing more accurately than females. However, due to our experimental design, we cannot conclusively determine whether these differences are indeed cognitive or influenced by confounding factors during the group phase of the experiment.

There is a growing recognition of the influence of both genetic and ecological context in shaping different cognitive traits. The hippocampal region is identified as a critical area for memory and learning in mammals, susceptible to modification by environmental influences. Although previous studies have identified the effects of various factors on cognitive parameters during early development, comparatively few research was conducted on wild species to analyze the role of natural environmental stimuli in the formation of spatial learning and memory abilities. Thus, to assess the importance of exposure to a complex and challenging environment during early development, we compared spatial learning performance of captive-born tuco-tucos with previous data obtained in our laboratory from wild-born adult tuco-tucos. The results showed that wild-born individuals learned faster, requiring less time to complete a labyrinth and making fewer errors than those who had no experience in their natural environment. These findings underscore the importance of considering ecological factors in understanding the evolution of brains and cognitive abilities.

Three experiments were conducted to investigate Conditioned Olfactory Preferences using orthonasal inhalation, which is a less explored perceptual pathway compared to retronasal inhalation. In these experiments, odors were impregnated onto plastic disks to prevent the subjects from consuming or tasting them. The reinforcers used were a sucrose solution (Caloric groups) and a saccharin solution (Non-Caloric groups). The influence of nutritional deprivation was analyzed, with unrestricted access to food throughout the procedure in Experiment 1, food restriction during the conditioning phase in Experiment 2, and limited access to food during the test phase in Experiment 3. The results revealed conditioned preferences using both sucrose and saccharin as reinforcers. Furthermore, dietary restriction reduced the conditioned preference induced by saccharin, but not the preference induced by sucrose. These findings are discussed in light of the potential differences between orthonasal and retronasal presentation of odors during conditioning.

American black bears (Ursus americanus) may be more social than currently understood. We used long-term video and genetic data to evaluate social interactions among wild, independent-aged black bear on a conservation property in western Montana, USA. We used multinomial logistic regression to evaluate predictions about male-male interactions within the context of individual fitness, female-female interactions within the context of inclusive fitness, and male-female interactions within the context of female counterstrategies to infanticide. Overall, our findings challenged the assumption that independent-aged bears interact only during the mating season or when concentrated feeding sites are present. We documented 169 interaction events by at least 66 bear pairs, 92 (54%) of which occurred outside of the peak mating season and in the absence of concentrated feeding sites. The probability that male-male pairs engaged in play and other non-agonistic behaviours was higher than that for female-female pairs. Conversely, the probability that female-female pairs engaged in chase behaviour was higher than that for male-male and male-female pairs. We documented evidence of female mate choice, female resource defense, sexually selected infanticide (SSI), and female counterstrategies to avoid SSI. Our findings improve our understanding of ursid ethology and underscore the complexity of ursid sociality.

How stimulant drugs affect risky choice and the role of reinforcement magnitude has been an important question for research on impulsivity. This study investigated rats’ responding on a rapid acquisition, concurrent chains, probability discounting task under methamphetamine administration. In each block of four sessions, probability of reinforcement delivery was unequal (0.5/1.0, 1.0/0.5) or equal, (1.0/1.0, 0.5/0.5) while amount of reinforcement was constant and unequal. This allowed for an estimate of probability discounting and the magnitude effect (where larger reinforcers are discounted at a greater rate) in each block. Baseline, acute and chronic methamphetamine administration, and re-establish baseline phases were completed. Rats showed sensitivity to probability and magnitude in baseline, as well as a magnitude effect whereby preference for the larger reinforcement was greater with 100% than 50% reinforcement probability. Acute methamphetamine dose-dependently reduced the probability effect. There were no effects of chronic administration and only probability discounting was maintained in the re-establish baseline phase. This was the first procedure to find a magnitude effect with rats in a probability discounting procedure and demonstrates that acute methamphetamine reduces both the probability and magnitude effects which increases propensity for risky choice.

This study was conducted to determine the senses that facilitate prey detection in the marble goby (Oxyeleotris marmorata) larvae. The ingestion ratios of live (generate chemical and mechanical stimuli) or frozen Artemia nauplii (generate chemical but no mechanical stimuli) by the intact or free neuromast (mechanoreceptor)-ablated O. marmorata larvae (11 mg/L streptomycin treatment before feeding) under the light or dark (fish vision was obstructed) condition were examined. Vision, mechano-, and chemoreceptions were all found to be essential in prey detection of the O. marmorata larvae. Prey movement has a significant influence as a visual stimuli on the O. marmorata larval feeding as the Artemia nauplii ingestion ratio was approximately 40% higher with significant (p = 0.001, d = 3.0), when the intact larvae were fed with the live (78.1 ± 1.5%), rather than the frozen (40.9 ± 2.8%) Artemia nauplii, under the light condition. This result was assured when no significant difference (p = 0.572, d = 0.2) was found between the ingestion ratios of frozen Artemia nauplii by the intact O. marmorata larvae under light and dark conditions. These findings demonstrate that prey detection in the O. marmorata larvae was facilitated by multi-modal senses, allowing O. marmorata larvae to survive in their natural habitats.

Across three experiments, participants underwent conditioning sequences where the self-referential term I AM (Conditioned Stimulus, or CS+) or a scrambled counterpart M IA (CS-) was paired with either neutral (Unconditioned Stimulus, or US-) or positive attributes (US+). CS and US were presented under subliminal and/or visible conditions. A normalized indicator of affective shift and an explicit self-esteem measure were deployed as outcome measures. In Experiment 1 (N = 60), subliminal CS+ followed by visible US+ produced a significant affective shift only. Experiment 2 (N = 59) presented CS and US under subliminal conditions, which did not influence either outcome measure. In Experiment 3 (N = 60), visible CS appeared with visible US, which resulted in a significant effect on explicit self-esteem only. These findings highlight the central roles of CS and/or US visibility towards influencing reported affect and self-esteem. We theorize that configural components of subliminally presented stimuli can become perceptually encoded and influence self-related affect non-consciously.