Biocatalysis and agricultural biotechnology最新文献_第10页

Biochemical and physiological priming by silica nanoparticles enhances early defense in Coffea arabica var. Bourbon 生化和生理启动二氧化硅纳米颗粒增强早期防御阿拉比卡咖啡品种波旁

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-13 DOI: 10.1016/j.bcab.2025.103853

Alexis Salazar-Navarro , Víctor Ruíz-Valdiviezo , Gregorio Joya-Dávila , Andy Gómez-Valdéz , Daniel Gonzalez-Mendoza , Benjamín Valdez-Salas , Ali Abdelmoteleb

This study evaluated the biochemical and physiological responses of Coffea arabica var. Bourbon to foliar application of silica nanoparticles (SiNPs) at concentrations of 1.5, 3, 6, and 12 mM under greenhouse conditions. Treatments were arranged in a completely randomized design with four replicates, and analyses were performed 72 h post-application. Physiological parameters included chlorophyll content (Soil Plant Analysis Development, SPAD), chlorophyll fluorescence (maximum quantum yield of photosystem II, Fv/Fm) and non-photochemical quenching (NPQ), electrolyte leakage (EL), and membrane stability index (MSI). Biochemical assays evaluated the activity of defense-related enzymes: phenylalanine ammonia-lyase (PAL), chitinase, β-1,3-glucanase, peroxidase, and catalase. The 6 and 12 mM SiNPs treatments significantly enhanced enzymatic activity, particularly catalase, chitinase, and glucanase at 6 mM, and PAL and peroxidase at 12 mM, suggesting activation of salicylic acid–mediated defense pathways. The 1.5 mM treatment resulted in the lowest EL values and the highest MSI, indicating improved cell integrity without phytotoxic effects. Although Fv/Fm and NPQ values did not differ significantly among treatments, plants treated with 1.5 and 3 mM SiNPs showed favorable trends in photosystem performance, maintaining stable photochemical efficiency and reduced energy dissipation. To our knowledge, this is the first report demonstrating biochemical evidence consistent with SiNPs-induced priming in Coffea arabica, highlighting their potential as elicitors for enhancing crop resilience and supporting integrated disease management strategies.

本研究评估了温室条件下，叶面施用浓度分别为1.5、3、6和12 mM的纳米二氧化硅（SiNPs）对波本咖啡（Coffea arabica var. Bourbon）的生化和生理反应。处理采用完全随机设计，共4个重复，在施用72 h后进行分析。生理参数包括叶绿素含量（Soil Plant Analysis Development， SPAD）、叶绿素荧光（光系统II的最大量子产率，Fv/Fm）和非光化学猝灭（NPQ）、电解质泄漏（EL）和膜稳定性指数（MSI）。生化分析评估了防御相关酶的活性：苯丙氨酸解氨酶（PAL）、几丁质酶、β-1,3-葡聚糖酶、过氧化物酶和过氧化氢酶。6和12 mM SiNPs处理显著提高了酶活性，特别是6 mM的过氧化氢酶、几丁质酶和葡聚糖酶，以及12 mM的PAL和过氧化物酶，表明激活了水杨酸介导的防御途径。1.5 mM处理导致最低的EL值和最高的MSI，表明细胞完整性得到改善，但没有植物毒性作用。虽然不同处理间Fv/Fm和NPQ值差异不显著，但1.5和3 mM SiNPs处理的植株表现出良好的光系统性能，保持了稳定的光化学效率，减少了能量耗散。据我们所知，这是第一份证明与sinps诱导的阿拉比卡咖啡启动一致的生化证据的报告，强调了它们作为提高作物抗灾能力和支持综合疾病管理策略的激发子的潜力。

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引用次数: 0

Comparative effects of nano and conventional molybdenum fertilizers on oilseed rape growth and yield under acidic soil conditions 酸性土壤条件下纳米钼肥与常规钼肥对油菜生长和产量的影响比较

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-12 DOI: 10.1016/j.bcab.2025.103857

Muhamad Syaifudin , Mohamed G. Moussa , Muhammad Shoaib Rana , Parashuram Bhantana , Yiwen Wang , Chengxiao Hu , Xuecheng Sun

Oilseed rape (Brassica napus L.) often experiences molybdenum (Mo) deficiency, which impairs growth and yield-traits, particularly in acidic soils. Nano Mo fertilizers, offering enhanced absorption and targeted delivery, present a promising solution for Mo deficiency, especially prevalent in acidic soils. This study evaluated the effects of foliar-applied nano potassium molybdate (nano MoK) on Mo accumulation, growth, and yield in oilseed rape, compared to conventional Mo fertilizers. A pot experiment was conducted with five foliar treatments: control (no Mo), nano MoK, potassium molybdate (MoK), ammonium molybdate (MoA), and potassium (K). Compared to the control, all Mo treatments significantly increased Mo content and accumulation in stems, glumes, and seeds, as well as chlorophyll b, intercellular CO₂ concentration (C_i), seed dry weight, seed yield, and harvest index. Crucially, nano MoK significantly enhanced chlorophyll b content by 25.3 % compared to MoK, and led to a pronounced increase in pods per plant, specifically 29.3 % higher than MoK and 25.6 % higher than MoA. While Mo accumulation in seeds was highest under nano MoK, it was significantly greater only when compared to MoA. Principal Component Analysis (PCA) confirmed nano MoK's distinct and overall positive influence on growth and yield components relative to conventional Mo sources. These findings suggest that foliar-applied nano MoK demonstrates enhanced effectiveness compared to conventional Mo fertilizers, particularly in specific physiological responses and key reproductive traits like pod formation. Its pronounced impact on reproductive development underscores its potential as a targeted strategy to mitigate Mo deficiency and significantly improve oilseed rape productivity.

油菜（Brassica napus L.）经常经历钼（Mo）缺乏，这损害了生长和产量性状，特别是在酸性土壤中。纳米Mo肥料具有增强吸收和定向输送的特点，是解决酸性土壤中普遍存在的Mo缺乏的一种很有前景的解决方案。研究了叶面施用纳米钼酸钾（nano MoK）对油菜钼积累、生长和产量的影响，并与常规钼肥进行了比较。盆栽试验采用对照（无Mo）、纳米MoK、钼酸钾（MoK）、钼酸铵（MoA）和钾(K) 5种叶面处理。与对照相比，各Mo处理均显著提高了水稻茎、颖花和种子中Mo含量和积累量，提高了叶绿素b、细胞间CO2浓度（Ci）、种子干重、产量和收获指数。最重要的是，与MoK相比，纳米MoK显著提高了叶绿素b含量25.3%，导致单株荚果显著增加，比MoK高29.3%，比MoA高25.6%。在纳米MoK下，Mo在种子中的积累量最高，但只有在MoA下才显著高于MoK。主成分分析（PCA）证实，相对于传统Mo源，纳米MoK对生长和产量成分有明显的整体正向影响。这些发现表明，叶面施用纳米MoK比传统的钼肥具有更高的有效性，特别是在特定的生理反应和关键的生殖性状（如豆荚形成）方面。它对生殖发育的显著影响强调了其作为缓解钼缺乏和显著提高油菜生产力的有针对性战略的潜力。

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引用次数: 0

Biodiesel production from Ulva lactuca: Insights into kinetic and thermodynamic evaluation of lipid transesterification 从乳脂树生产生物柴油：对脂质酯交换动力学和热力学评价的见解

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-12 DOI: 10.1016/j.bcab.2025.103856

Fozy Binhweel , Mardiana Idayu Ahmad , Abdul Khalil H.P.S , Mohammad Aliff Shakir

The escalating demand for sustainable energy necessitates innovative and eco-friendly alternatives, positioning algal biomass such as Ulva lactuca as a potential feedstock for biodiesel production. A major challenge in optimizing biodiesel production from Ulva lactuca lies in the limited understanding of the reaction kinetics and thermodynamics governing the transesterification process. These factors play a crucial role in determining reaction efficiency and energy requirements. This study addresses this challenge by conducting a detailed evaluation of the first-order rate kinetic and thermodynamic parameters governing the transesterification reaction. Ulva lactuca samples were dried and ground before undergoing lipid extraction using Soxhlet extraction method, yielding 7.60 ± 0.10 % lipids. The extracted lipids were converted into biodiesel via base-catalyzed transesterification under controlled reaction conditions. First-order kinetic and thermodynamic modelling was applied to evaluate rate constant, activation energy (E_a), enthalpy change (ΔH), entropy change (ΔS), and Gibbs free energy change (ΔG). The analyses revealed an activation energy of 46.35 kJ/mol, enthalpy change of 43.64 kJ/mol, entropy change of −149.98 J/mol·K, and Gibbs free energy change ranging from 91.24 to 93.50 kJ/mol across different conditions. These findings confirm that the transesterification reaction is temperature-dependent, endothermic, and nonspontaneous, with reduced system disorder. This study enhances the fundamental understanding of the kinetic and thermodynamic parameters related to the transesterification of Ulva lactuca lipids, reinforcing its use for biodiesel production.

对可持续能源不断增长的需求需要创新和环保的替代品，将藻类生物质（如Ulva lactuca）定位为生物柴油生产的潜在原料。优化从紫叶树生产生物柴油的一个主要挑战在于对反应动力学和控制酯交换过程的热力学的有限理解。这些因素在决定反应效率和能量需求方面起着至关重要的作用。本研究通过对控制酯交换反应的一级速率动力学和热力学参数进行详细评估来解决这一挑战。Ulva lactuca样品干燥研磨后，采用索氏提取法提取脂质，脂质得率为7.60±0.10%。在控制的反应条件下，通过碱催化酯交换反应将提取的油脂转化为生物柴油。采用一级动力学和热力学模型计算了反应的速率常数、活化能（Ea）、焓变（ΔH）、熵变（ΔS）和吉布斯自由能变化（ΔG）。结果表明，不同反应条件下的活化能为46.35 kJ/mol，焓变为43.64 kJ/mol，熵变为- 149.98 J/mol·K，吉布斯自由能变化范围为91.24 ~ 93.50 kJ/mol。这些发现证实了酯交换反应是温度依赖的、吸热的、非自发的，系统紊乱程度较低。本研究提高了对乳酸脂质的酯交换反应动力学和热力学参数的基本认识，加强了其在生物柴油生产中的应用。

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引用次数: 0

Optimizing xanthan gum production using okara and response surface methodology: Unlocking sustainable biopolymer production 利用黄原胶和响应面方法优化黄原胶生产：解锁可持续生物聚合物生产

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-11 DOI: 10.1016/j.bcab.2025.103852

Richard Vincent Asase, Tatiana Vladimirovna Glukhareva

Xanthan gum is a commercially important exopolysaccharide with production influenced by raw materials costs and nutrient availability. This study optimized okara, glucose and KH₂PO₄ concentrations for xanthan gum production by Xanthomonas campestris B6720 using response surface methodology combined with a desirability function. A maximum yield of 29.137 g/L was achieved in a confirmatory run under the optimized conditions of 10, 0.2, and 2 g/100 ml for okara, KH₂PO₄, and glucose respectively. Structural characterization using FTIR, ¹H NMR, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) compared xanthan gum produced from okara (OKAXG), standard media (STDXG), and a commercial source (COMXG). Principal component analysis (PCA) of FTIR data revealed distinct structural differences, with OKAXG being unique. TGA showed initial mass losses of approximately 12 % water evaporation, followed by major degradation mass losses of 38.14 % (COMXG), 53.49 % (STDXG), and 45.06 % (OKAXG). Derivative thermogravimetry indicated sharper decomposition peaks for COMXG and STDXG and broader peaks for OKAXG, suggesting enhanced thermal stability. Elemental analysis showed significant differences (p < 0.05) in carbon, hydrogen, and nitrogen contents among the xanthan gum samples except for hydrogen between STDXG and COMXG. Viscosity measurements showed lower viscosity for STDXG and OKAXG than for COMXG. Antioxidant activity by DPPH scavenging was highest in COMXG (65 %), followed by OKAXG (55 %) and STDXG (48 %). These findings indicate that the production substrate influences xanthan gum's molecular structure, thermal behavior, and bioactivity. Okara as an alternative nutrient source offers a promising, sustainable, and cost-effective approach to xanthan production by valorizing agro-industrial by-products.

黄原胶是一种重要的商业外多糖，其生产受原料成本和养分利用率的影响。本研究利用响应面法结合期望函数优化了油菜黄单胞菌B6720生产黄原胶所需的okara、葡萄糖和KH2PO4浓度。在10、0.2和2 g/100 ml的优化条件下，对菜心、KH2PO4和葡萄糖进行验证，产率最高可达29.137 g/L。利用FTIR、1H NMR、热重分析（TGA）和差示扫描量热法（DSC）对由okara （OKAXG）、标准培养基（STDXG）和商业来源（COMXG）生产的黄原胶进行了结构表征。主成分分析（PCA）显示出明显的结构差异，其中OKAXG是唯一的。TGA显示初始质量损失约为12%，其次是主要降解质量损失38.14% (COMXG), 53.49% （STDXG）和45.06% （OKAXG）。导数热重分析表明，COMXG和STDXG的分解峰更陡，OKAXG的分解峰更宽，表明其热稳定性增强。元素分析表明，除氢外，STDXG和COMXG黄原胶样品的碳、氢、氮含量差异显著（p < 0.05）。粘度测量表明，STDXG和OKAXG的粘度低于COMXG。COMXG对DPPH的清除活性最高（65%），其次是OKAXG（55%）和STDXG（48%）。这些发现表明，生产底物影响黄原胶的分子结构、热行为和生物活性。Okara作为一种替代营养来源提供了一个有前途的，可持续的，具有成本效益的方法黄原胶生产的农业工业副产品。

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引用次数: 0

Citrus Peel–Mediated ZnO Nanoparticles: Green synthesis, plant responses, and antibacterial activity 柑橘皮介导的ZnO纳米颗粒：绿色合成、植物反应和抗菌活性

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-11 DOI: 10.1016/j.bcab.2025.103854

Yağmur Uysal, Zeynep Görkem Doğaroğlu

The pursuit of sustainable agricultural practices has spurred interest in eco-friendly nanomaterials. This study investigates the green synthesis of zinc oxide nanoparticles (ZnO-NP) using peel extracts of orange (Citrus sinensis, O-) and bitter orange (Citrus aurantium, BO-), abundant agro-wastes as an alternative to conventional chemical methods. The synthesized nanoparticles were characterized; XRD confirmed a hexagonal wurtzite structure and FTIR indicated phytochemical capping. Key quantitative outcomes were as follows: Average crystallite sizes (Debye–Scherrer) were 26.78 nm (raw), 23.34 nm (O-), and 19.84 nm (BO-). In germination tests, raw ZnO-NP achieved 100 % germination at 2000 mg L⁻¹, while higher concentrations decreased performance. BO-ZnO-NP markedly reduced root–shoot elongation at elevated levels, indicating phytotoxicity. In foliar application, raw and BO-ZnO-NP at 1000–2000 mg L⁻¹ increased chlorophyll and promoted growth; chlorophyll peaked at 5000 mg L⁻¹ (raw) and 1000 mg L⁻¹ (BO-). Biomass followed a dose-dependent pattern: raw ZnO-NP increased biomass above 3000 mg L⁻¹, whereas BO-ZnO-NP reduced biomass. Maximum Zn accumulation occurred under soil application at 3000 (raw), 4000 (O-), and 5000 (BO-) mg L⁻¹, and under foliar application at 3000 (raw), 2000 (O-), and 4000 (BO-) mg L⁻¹, respectively. Antibacterial tests showed greater sensitivity of S. aureus to O-/BO-ZnO-NP at low doses compared to E. coli, while raw ZnO-NP exhibited the highest overall inhibition. These findings highlight the importance of synthesis route and application strategy in maximizing the agronomic benefits of ZnO-NP while minimizing phytotoxic and environmental risks.

对可持续农业实践的追求激发了人们对生态友好型纳米材料的兴趣。本研究以柑橘（Citrus sinensis, O-）和苦橙（Citrus aurantium, BO-）这两种丰富的农业废弃物为原料，研究了绿色合成氧化锌纳米粒子（ZnO-NP）的方法。对合成的纳米颗粒进行了表征；XRD证实为六方纤锌矿结构，FTIR证实为植物化学盖层。主要定量结果如下：平均晶粒尺寸（Debye-Scherrer）为26.78 nm（生晶）、23.34 nm （O-）和19.84 nm （BO-）。在萌发试验中，粗ZnO-NP在2000 mg L−1条件下萌发率达到100%，但浓度越高则萌发率越低。BO-ZnO-NP水平升高显著降低了根冠伸长，表明植物毒性。在叶面施用中，1000 ~ 2000 mg L−1的生料和BO-ZnO-NP增加了叶绿素，促进了生长；叶绿素峰值为5000 mg L−1（生）和1000 mg L−1 （BO-）。生物量遵循剂量依赖模式：在3000 mg L−1以上，原始ZnO-NP增加了生物量，而BO-ZnO-NP减少了生物量。土壤施锌量分别为3000（生）、4000 （O-）和5000 (BO-) mg L - 1，叶面施锌量分别为3000（生）、2000 （O-）和4000 (BO-) mg L - 1。抗菌试验显示，与大肠杆菌相比，低剂量的金黄色葡萄球菌对O-/BO-ZnO-NP更敏感，而未经处理的ZnO-NP表现出最高的总体抑制作用。这些发现强调了合成路线和应用策略对于最大限度地提高ZnO-NP的农艺效益，同时最大限度地降低植物毒性和环境风险的重要性。

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引用次数: 0

Fractionation of polyphenolic extract from walnut male flowers (Juglans regia L.) and evaluation of biological activity towards cancer cells 核桃雄花多酚提取物的分离及抗肿瘤活性评价

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-08 DOI: 10.1016/j.bcab.2025.103850

Natalia Żurek, Ireneusz Kapusta

The bioactive properties of male walnut flowers are among the least understood of all morphological parts of the Juglans regia tree. In this study, C18 bed fractionation technology was used for the first time to define the component or fraction of flowers with the highest cytotoxic activity against cancer cells and antioxidant activity. The work resulted in a crude extract (CE) and four fractions with significantly different profiles and polyphenolic compositions. A total of 32 phenolic compounds were identified, including phenolic acids, flavonoids, juglanoid isomers, and tannins. Their content in CE was 22.58 mg/g and was 6.3, 9.9, and 2.1 times higher in fractions 1, 2, and 3, respectively. In contrast, for fraction 4, this value was 3.4 times lower. Cytotoxic activity against six cancer cell lines can be ranked in the following order: fraction 4 > fraction 3 > fraction 2 > fraction 1 > CE. The most active fraction showed cytotoxicity 1.7 to 19.4 times weaker than cisplatin. Fractions 2 and 4 also showed the highest anti-migration (inhibition of 95.8 % and 86.0 %, respectively) and anti-invasive (92.2 % and 85.0 %, respectively) potential. In turn, the antioxidant activity in the chemical system significantly depended on the content of phenolic compounds and was ordered as follows: fraction 2 > fraction 3 > fraction 1 > fraction 4 > CE. This activity was also confirmed in cell tests (CCD841CoN, RAW 246.7 lines) through its ability to protect against cellular stress, ROS and NO generation. These findings allow for a comprehensive understanding of the influence of the phytochemical composition of walnut flowers on biological activity and provide insight into its potential therapeutic applications.

雄性核桃花的生物活性特性是在核桃树的所有形态部分中最不为人所知的。本研究首次采用C18床分离技术确定了花中具有最高细胞毒活性和抗氧化活性的成分或部位。这项工作产生了一个粗提取物（CE）和四个馏分具有显著不同的剖面和多酚成分。共鉴定出32种酚类化合物，包括酚酸、类黄酮、类核桃素异构体和单宁。其在CE中的含量为22.58 mg/g，在馏分1、2和3中分别高出6.3、9.9和2.1倍。相比之下，对于分数4，这个值要低3.4倍。对六种癌细胞的细胞毒活性可按以下顺序排列：部分4 >；部分3 >；部分2 >；部分1 >； CE。最活性部位的细胞毒性较顺铂弱1.7 ~ 19.4倍。组分2和组分4的抗迁移活性最高，分别为95.8%和86.0%，抗侵袭活性最高，分别为92.2%和85.0%。化学体系中的抗氧化活性显著依赖于酚类化合物的含量，顺序为：组分2 >；组分3 >；组分1 >；组分4 >； CE。这种活性也在细胞试验（CCD841CoN, RAW 246.7系）中得到证实，因为它具有抵抗细胞应激、ROS和NO生成的能力。这些发现有助于全面了解核桃花的植物化学成分对生物活性的影响，并为其潜在的治疗应用提供见解。

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引用次数: 0

Himalayan botanical mediated elicitation regulates wheat growth through secondary metabolites and hormonal signalling 喜马拉雅植物介导的诱导通过次生代谢物和激素信号调节小麦生长

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-07 DOI: 10.1016/j.bcab.2025.103851

Seema Devi, Yashoda Nandan Tripathi, Richa Raghuwanshi

Plants as a resource of novel elicitors capable of inhibiting pathogen and promoting crop growth are gaining significant attention. Himalayan plants (Colebrookea oppositifolia, Digitalis purpurea and Rumex hastatus), well documented for their pharmacological value and antimicrobial properties were studied for their growth promoting potentials on wheat. Methanolic crude extract obtained from leaf samples of the selected Himalayan plants were applied on wheat through seed priming and foliar spray method. Morphological parameters selected for phyto-stimulatory assessment of treatments were germination percent, shoot length, root length, seedling fresh weight and dry weight, vigour index, stem thickness and root volume, where significant improvements were recorded. Biochemical changes in response to treatment measured were photosynthetic pigments, total phenolic and flavonoid content, osmolytes and antioxidant activity. Wheat plants showed an increment in all the parameters after treatment with botanicals. The growth-promoting potentials of the selected botanicals were further evaluated for quantifying endogenous phytohormone (IAA and GA) levels in wheat through High-Performance Liquid Chromatography (HPLC). Increased expression of hormones was recorded in wheat, which was treatment specific. Wheat stems examined through transverse sections showed increased lignification around the vascular tissue and hypodermis. This study represents the first documented investigation into the growth-promoting effects of selected botanicals on wheat, providing novel insights into their potential as biostimulants.

植物作为一种能够抑制病原菌和促进作物生长的新型激发子资源正受到越来越多的关注。对喜马拉雅植物（colebrokea oppositifolia， Digitalis purpurea和Rumex hastatus）的药理价值和抗菌特性进行了研究，研究了它们对小麦的促生长潜力。选用喜马拉雅植物叶片样品提取的甲醇粗提物，通过种子灌种和叶面喷雾的方法施用于小麦上。用于植物刺激评价的形态学参数为发芽率、茎长、根长、幼苗鲜重和干重、活力指数、茎粗和根体积，其中有显著改善。光合色素、总酚和类黄酮含量、渗透物和抗氧化活性等生化指标对处理的影响。小麦植株经植物药处理后，各项指标均有增加。利用高效液相色谱法（HPLC）测定小麦内源植物激素（IAA和GA）水平，进一步评价所选植物制剂的促生长潜力。小麦中记录到激素表达增加，这是处理特异性的。小麦茎横切面显示维管组织和皮下组织周围木质化增加。本研究首次对选定植物药对小麦的促生长作用进行了文献调查，为其作为生物刺激素的潜力提供了新的见解。

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引用次数: 0

Kombucha fermentation enhances bioactive profile and sensory quality of sea buckthorn leaf infusion 康普茶发酵提高沙棘叶浸剂的生物活性和感官品质

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-06 DOI: 10.1016/j.bcab.2025.103849

Nikita Sanwal, Mohammed A. Bareen, Jatindra K. Sahu

The sea buckthorn leaves (SBL) possess significant medicinal properties, making them a promising substrate for developing a functional beverage. A symbiotic culture of bacteria and yeast (SCOBY) was used to ferment the SBL infusion at 30 ± 2 °C, producing kombucha; phytoconstituent dynamics were monitored on days 3, 7, 10, and 14. Principal component analysis separated day-0 infusions from day-14 kombuchas and distinguished the two species (PC1 55.72 %, PC2 21.91 %), reflecting lower pH, higher titratable acidity, higher TPC/TFC, and species-specific shifts in organic acids and phenolics. Polyphenolic compounds such as epigallocatechin, kaempferol, hamamelitannin, rutin, and quercetin increased significantly (p < 0.05). Cu and Zn levels decreased (Log2 change −0.51 and −0.18), while Mg, K, Ca, and Ni increased (Log2 change 0.201, 0.15, 0.44, 0.41). Fe content was augmented from 0 to 70.51 ppb because of kombucha cultivation in the SBL infusion. Fermentation enhanced titratable acidity, reduced pH, and produced flavour compounds like 3-methylmannoside, xanthosine, and diisopropyl silyl isopropyl ether. This study highlights the nutritional, flavour, and valorisation potential of SBL in beverage development.

沙棘叶具有重要的药用价值，是开发功能饮料的重要原料。采用细菌与酵母菌共生培养（SCOBY），在30±2℃条件下对SBL输注进行发酵，制得康普茶；在第3、7、10和14天监测植物成分动态。主成分分析分离出第0天和第14天的红茶菌，并区分出两个物种（PC1 55.72%, PC2 21.91%），反映出较低的pH值、较高的可滴定酸度、较高的TPC/TFC，以及有机酸和酚类物质的物种特异性变化。表没食子儿茶素、山奈酚、金缕梅单宁、芦丁和槲皮素等多酚类化合物显著增加（p < 0.05）。Cu和Zn水平降低（Log2变化为- 0.51和- 0.18），Mg、K、Ca和Ni水平升高（Log2变化为0.201、0.15、0.44、0.41）。在SBL输注中加入康普茶后，铁含量由0提高到70.51 ppb。发酵提高了可滴定的酸度，降低了pH值，并产生了风味化合物，如3-甲基甘露糖苷，黄嘌呤和二异丙基硅基异丙基醚。这项研究强调了SBL在饮料开发中的营养、风味和增值潜力。

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引用次数: 0

Beneficial cyanobacteria as seed coatings to enhance the storability and viability indices of spinach seeds 有益蓝藻作为种子包衣提高菠菜种子的贮藏性和活力指标

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-05 DOI: 10.1016/j.bcab.2025.103844

Ravi Mourthy Nivedha , Radha Prasanna , Mujtahida Khatun , Deepti Varsha , Akanksha Bhardwaj , Sandeep Kumar Lal , Sudipta Basu , Samrat Rej , Awani Kumar Singh , Yashbir Singh Shivay

Cyanobacteria, known for their carbon- and nitrogen-fixing abilities, can be beneficial as biofertilizers and growth stimulating options in agriculture; hence, their use as seed coatings can be a viable strategy for sustainable agriculture. Therefore, this study aimed to evaluate and compare the storability of spinach seeds coated with three different cyanobacterial formulations by assessing germination-related biometric and physiological attributes at monthly intervals over a twelve-month storage period. Biometric indices were enhanced by 15–40 % in the coated seeds, with a significant increase in germination and vigor indices of 46–83 %. After 12 months of storage, seeds from each treatment were sown in nursery protrays placed under climate-controlled conditions. Their viability, growth, nutrient profile, and enzyme activities related to C-N metabolism were compared with control plants. Significantly greater values (20–22 %) were recorded in terms of germination indices, fresh/dry weight, leaf enzymatic activities, and micronutrient content in plants derived from coated seeds. Overall, all the cyanobacterial treatments enhanced seed storability indices and plant performance, demonstrating their potential as effective seed coating interventions towards improving shelf life and sustaining the quality traits.

蓝藻以其固定碳和氮的能力而闻名，在农业中可以作为生物肥料和刺激生长的选择；因此，将它们用作种子包衣是可持续农业的一种可行策略。因此，本研究旨在评估和比较三种不同蓝藻配方包被的菠菜种子的可储存性，通过评估发芽相关的生物特征和生理属性，每个月间隔12个月的储存期。包被种子的生物特征指数提高了15 ~ 40%，萌发和活力指数显著提高了46 ~ 83%。经过12个月的储存，每个处理的种子在气候控制条件下被播种在苗圃中。并与对照植株进行了活力、生长、营养成分和碳氮代谢相关酶活性的比较。包衣种子获得的植物在发芽指数、鲜/干重、叶片酶活性和微量营养素含量方面均有显著提高（20 - 22%）。总体而言，所有蓝藻处理都提高了种子的储存性指标和植株性能，表明它们有潜力作为有效的种子包衣干预措施，提高种子的保质期和保持品质性状。

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引用次数: 0

Phytomolecule-doped green synthesis of Ag@Fe bimetallic nanocomposites from Abutilon indicum leaf extract: Antimicrobial activity against Mycobacterium spp. and pathogenic bacteria and their cytotoxicity assessment in zebrafish embryos 紫竹叶提取物Ag@Fe双金属纳米复合材料的植物分子掺杂绿色合成：对斑马鱼胚胎分枝杆菌和致病菌的抑菌活性及其细胞毒性评价

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-11-05 DOI: 10.1016/j.bcab.2025.103846

Mary Shamya Arokiarajan , John Christopher J. , Ranjani S. , Wasim Akram S.A. , Ramkumar K.M. , Tirumala Santoshkumar S. , Noman Anwar , Rajaseker T. , M Radhakrishnan , Muzammil Ahmed V. , Murali Krishna Prasad , Kabiruddin Ahmed K. , Zaheer Ahmed N. , Rampratap Meena

Plants utilize phytomolecules as both reducing and capping agents in the green synthesis of nanoparticles. Ag@Fe bimetallic nanocomposites have attracted significant attention due to their unique properties. However, the presence of harmful surface compounds is challenging to eliminate, reducing their biocompatibility and limiting their potential biological applications. In this study, Ag@Fe nanocomposites were synthesized using a green approach, employing Abutilon indicum leaf extract to coat the nanocomposites with phytomolecules for enhanced antibacterial activity against pathogenic bacterial strains. The antibacterial efficacy was assessed using the agar well diffusion method, and the minimum inhibitory concentration (MIC) was determined and compared with a standard drug and plant extract. The synthesized nanocomposites were characterized using XRD, FTIR, SEM, EDX, and TEM. Their toxicity was evaluated using zebrafish embryos and the MDA-MB-231 cell line. Phytomolecule-coated Ag@Fe nanocomposites exhibited strong antibacterial activity, effectively inhibiting Methicillin-resistant Staphylococcus aureus (MRSA) at 0.312 μg/mL and Mycobacterium tuberculosis H37Rv, showing 84 % inhibition at 200 μg/mL. Additionally, they demonstrated cytotoxic effects on the MDA-MB-231 cell line, with the lowest cell viability recorded at 83.3 ± 0.02 % at 5 μg/mL. Furthermore, phytomolecule-coated Ag@Fe NC's exhibited good biocompatibility, with 90 % cell viability in zebrafish embryos. The green synthesis of nanocomposites using Abutilon indicum leaf extract presents an efficient and cost-effective approach, yielding biocompatible and biologically active nanoparticles with promising therapeutic potential.

植物利用植物分子作为纳米颗粒绿色合成的还原和封盖剂。Ag@Fe双金属纳米复合材料以其独特的性能引起了人们的广泛关注。然而，有害表面化合物的存在很难消除，降低了它们的生物相容性，限制了它们潜在的生物应用。在本研究中，采用绿色方法合成了Ag@Fe纳米复合材料，利用Abutilon indicum叶片提取物在纳米复合材料表面包裹植物分子，增强了对病原菌菌株的抗菌活性。采用琼脂孔扩散法评价其抑菌效果，测定其最低抑菌浓度（MIC），并与标准药物和植物提取物进行比较。采用XRD、FTIR、SEM、EDX和TEM对合成的纳米复合材料进行了表征。使用斑马鱼胚胎和MDA-MB-231细胞系对其毒性进行了评估。植物分子包被Ag@Fe纳米复合材料具有较强的抗菌活性，对耐甲氧西林金黄色葡萄球菌（MRSA）的抑制作用为0.312 μg/mL，对结核分枝杆菌H37Rv的抑制作用为200 μg/mL，抑制率为84%。此外，它们对MDA-MB-231细胞系显示出细胞毒性作用，在5 μg/mL时，细胞存活率最低，为83.3±0.02%。此外，植物分子包被的Ag@Fe NC具有良好的生物相容性，在斑马鱼胚胎中具有90%的细胞存活率。利用Abutilon indicum叶片提取物绿色合成纳米复合材料是一种高效、经济的方法，可获得具有生物相容性和生物活性的纳米颗粒，具有良好的治疗潜力。

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引用次数: 0