Biocatalysis and agricultural biotechnology最新文献

{"title":"Phytochemical, cytotoxic, and insecticidal effects of crude extracts of the alga Alsidium triquetrum (SGGmelin) Trevisan on Aedes aegypti","authors":"Katiane Cruz Magalhaes Xavier ,&nbsp;Dayane Kelly Dias do Nascimento Santos ,&nbsp;Rômulo Carlos Dantas da Cruz ,&nbsp;Lidiane Quérolin Macena da Silva ,&nbsp;Alex Michel Silva Araújo ,&nbsp;PatryckÉrmerson Monteiro dos Santos ,&nbsp;Larissa Araújo Rolim ,&nbsp;Patrícia Maria Guedes Paiva ,&nbsp;Ivone Antônia de Souza ,&nbsp;Severino Alves Júnior","doi":"10.1016/j.bcab.2024.103461","DOIUrl":"10.1016/j.bcab.2024.103461","url":null,"abstract":"<div><div><em>Aedes aegypti,</em> the yellow fever mosquito, is the main vector of several arboviruses. It represents a public health problem and can develop resistance to chemical insecticides over time, requiring new formulations of ecologically friendly larvicides for its control. Seaweed is an abundant source of phytochemicals with diverse chemical compositions and is a prospective natural alternative to commercial insecticides. In this study, we aimed to investigate the larvicidal efficacy of the seaweed <em>Alsidium triquetrum</em> against <em>Ae. aegypti</em> by evaluating the toxic, morphological, and enzymatic effects of the ethanolic (AE) and hydroalcoholic (AH) extracts of the seaweed. The chemical composition of the extracts was evaluated using high-performance liquid chromatography and liquid chromatography coupled with high-resolution mass spectrometry, and the toxic effects were investigated by assessing the larvicidal activity, morphology of the larvae, and <em>in vitro</em> toxicity, along with enzymatic tests. The AH and AE extracts comprised primary and secondary metabolites, including amino and fatty acids and other compounds. The insecticidal bioassays showed larvicidal potential against <em>Ae. aegypti</em> at all concentrations tested, reaching 100% mortality in 24 h for both extracts. Morphological analysis of the larvae showed damage to the abdomen, changes in colour, disruption of the midgut, and loss of gill leaflets. The extracts stimulated similar activity for the enzymes amylase, trypsin, and acetylcholine esterase at concentrations above 0.5 μg mL⁻¹. The cytotoxicity of the algal extracts <em>in vitro</em> indicated non-toxicity at the concentrations tested. Therefore, both seaweed extracts are potential sources of new formulations for use as natural insecticides.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103461"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and fractionation of protein hydrolysate produced from fish sausage (Keropok Lekor) by-products by Lactobacillus casei fermentation: Optimisation and impact of peptide molecular weight on antioxidant and functional properties","authors":"Nur Yuhasliza Abd Rashid ,&nbsp;Musaalbakri Abdul Manan ,&nbsp;Khairul Faizal Pa'ee ,&nbsp;Nazamid Saari ,&nbsp;Fadzlie Wong Faizal Wong","doi":"10.1016/j.bcab.2024.103459","DOIUrl":"10.1016/j.bcab.2024.103459","url":null,"abstract":"<div><div>A comprehensive analysis of the functional properties of fish protein hydrolysate (FPH) is necessary before its incorporation into food products. This work aimed to optimise the fermentation process for the Malaysian fish sausage FBPs with <em>Lactobacillus casei</em> in terms of protein yield and antioxidant activities using response surface methodology (RSM). The second aim was to fractionate the FPH using ultrafiltration (UF) through 3, 5, and 10 kDa and to characterise the fractions in relation to peptide yield, antioxidant activities, and functional properties. The RSM optimisation elevated protein yield, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activities were effectively raised by 16.3%, 50.5%, and 21.7%, respectively. The DPPH (EC₅₀: 4.4 mg/mL) and ABTS (EC₅₀: 0.2 mg/mL) radical scavenging activities and protein solubility (79.9%) of the 10 kDa fraction were significantly higher than other fractions. However, lower MW fractions exhibited the highest emulsion activity index (5 kDa: 16.3 m²/g) and foaming expansion (3 kDa: 25.0%), which were 1.3 and 12.5 times higher than the FBPs, respectively. Surface hydrophobicity increased significantly in the FPH and UF fractions relative to FBPs by 23.9–26.3%, with no discernible difference between FPH and UF fractions. This study's findings can serve as a theoretical foundation and data in support of FBPs' optimisation of the fermentation process to produce bioactive FPH with high protein yield. The bioprocessing aspect outlined in the study significantly enhanced the antioxidant activity and functionality of the FPH. Therefore, boosting its potential for application as a healthy food ingredient.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103459"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential biomedical applications of biosurfactants derived from extremophilic bacteria: A review","authors":"Amir Mohammad Bagheri ,&nbsp;Mohammad Amin Raeisi Estabragh ,&nbsp;Abbas Pardakhty ,&nbsp;Ibrahim M. Banat ,&nbsp;Mandana Ohadi ,&nbsp;Bagher Amirheidari","doi":"10.1016/j.bcab.2024.103455","DOIUrl":"10.1016/j.bcab.2024.103455","url":null,"abstract":"<div><div>Surfactants are surface-active compounds mainly synthesized from petroleum. While widely used in various industrial applications, concerns about their economic and environmental impacts have led to the search for more sustainable alternatives, such as biosurfactants (BSs). BSs are diverse amphipathic molecules with unique chemical structures, synthesized by various microorganisms as secondary metabolites crucial for their survival. Extremophiles are a diverse group of microorganisms that thrive in extreme conditions and possess remarkable physicochemical properties and adaptability, making them excellent candidates for mining novel surface-active compounds and other molecules. Recent descriptions highlight how BSs from extremophilic bacteria possess properties that make them suitable for food, agriculture, pharmaceutical, and cosmetic applications. This review focuses on the biomedical applications of BSs from extremophilic bacteria, highlighting their anti-inflammatory, immunomodulatory, antimicrobial, antibiofilm, antiviral, anti-proliferative, wound healing, cosmeceutical, drug delivery, and green nanostructure synthesis properties.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103455"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Aschersonia placenta: Characterization and submerged cultivation in an air-lift bioreactor","authors":"Tarcisio M.S. Aragão ,&nbsp;Camila de S. Varize ,&nbsp;Tárcio S. Santos ,&nbsp;Sona Jain ,&nbsp;Marcelo da C. Mendonça","doi":"10.1016/j.bcab.2024.103463","DOIUrl":"10.1016/j.bcab.2024.103463","url":null,"abstract":"<div><div>The genus <em>Aschersonia</em> is readily found in natural epizootics against <em>Aleurocanthus woglumi</em>, an important pest in citrus crops commonly known as the citrus blackfly. It was one of the first microbiological pathogens studied in agricultural pest control. Host invasion begins with the attachment of fungal spores to the insect's cuticle, which is composed mainly of proteins and the polysaccharide chitin. However, <em>Aschersonia</em> is a fungus that is difficult to cultivate on a large scale, limiting its use in biological pest control programs. Therefore, this study aims to evaluate the production of <em>Aschersonia</em> in different solid and liquid culture media, investigate its potential for chitinase production, and finally evaluate the use of an air-lift bioreactor for the development of this fungus. We identified a fungus collected from a natural epizootic of <em>A. woglumi</em> and conducted a biochemical and molecular study focusing on the microbiological potential to produce chitinases. In addition, we investigated seven different additives (casein, yeast extract, pasteurized milk, chitin, <em>Manihot esculenta</em> starch and insect tegument) to accelerate and increase the growth of the isolated strain in synthetic culture media. We also investigated alternative production techniques, such as submerged cultivation in an orbital shaker and the air-lift bioreactor. Based on DNA sequencing and morphological analysis, the isolated fungus was identified as <em>Aschersonia placenta</em>. The solid culture with bromocresol purple dye showed higher enzymatic activity when cultured in media supplemented with chitin. Analysis of enzymatic activity in submerged cultivation confirmed that the addition of chitin increased chitinase production by more than fivefold compared to chitin-free broth cultivation. SDS-PAGE analysis allowed the identification of two regions at 37 and 115 kDa as possible chitinases. Submerged cultivation of <em>A. placenta</em> in an air-lift bioreactor resulted in production of 501.67 g L⁻¹ of wet biomass at 5 days (25 °C, 1.5 vvm aeration rate) using 20 g L⁻¹ dextrose, 4 g L⁻¹ potato broth, and 10 g L⁻¹ <em>Manihot esculenta</em> starch. These results support the suitability of submerged cultivation, especially in air-lift bioreactors, with the addition of <em>Manihot esculenta</em> starch in Potato Dextrose medium for the alternative production of <em>A. placenta</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103463"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of silk sericin/silk fibroin/Moringa oleifera-based hydrogel with antibacterial activity","authors":"Muhammad Iqbal Hidayat ,&nbsp;Andri Hardiansyah ,&nbsp;Riesca Ayu Kusuma Wardhani ,&nbsp;Khoiriah Khoiriah ,&nbsp;Evi Yulianti ,&nbsp;Muhammad Rayhan Izzati Yusuf ,&nbsp;Fahrialdi Fahrialdi","doi":"10.1016/j.bcab.2024.103482","DOIUrl":"10.1016/j.bcab.2024.103482","url":null,"abstract":"<div><div>Hydrogels with excellent antibacterial and mechanical properties represent ideal biomaterials for addressing skin defects, especially in burn wound dressings. In this study, PVA was combined with sericin (PVA/SS), fibroin (PVA/SF), sericin-<em>Moringa oleifera</em> (PVA/SS/MO), and fibroin-<em>Moringa oleifera</em> (PVA/SF/MO) to enhance the stability of the resulting hydrogel and have the antibacterial properties. Hydrogels were synthesized through freezing at −15 °C for 20 h and thawing at 5 °C for 4 h (freeze-thawing method), this process diminished residual waste substances and aligning with green chemistry principles. The FTIR analysis demonstrated that all hydrogel components were successfully blended. The resulting hydrogel had a rough surface with many uniform pores by SEM measurement. Hydrogels showed very hydrophilic properties with lower values of contact angle measurement with a range of 12 and 33°. The swelling properties of the hydrogel showed excellent performance and could absorb water for 5 days. The mechanical properties of the hydrogel were described as tensile strength of 0.29–0.77 MPa, tensile strain of 188–410%, elastic modulus of 0.18–0.72 MPa, and break elongation of 198–413%. Furthermore, PVA/SS/MO and PVA/SF/MO hydrogels display excellent antibacterial properties against Gram-positive (<em>Staphylococcus aureus</em>) and Gram-negative (<em>Escherichia coli</em>) bacteria. Thus, the developing hydrogel is expected to be a future candidate for addressing skin defect problems due to wound infection.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103482"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chamomile waste: A comprehensive insight on phytochemical and safety profile, extraction techniques and potential application","authors":"Nela Drača ,&nbsp;Krunoslav Aladić ,&nbsp;Marija Banožić ,&nbsp;Drago Šubarić ,&nbsp;Stela Jokić ,&nbsp;Ivana Nemet","doi":"10.1016/j.bcab.2024.103468","DOIUrl":"10.1016/j.bcab.2024.103468","url":null,"abstract":"<div><div>Chamomile is widely recognized as both an aromatic and medicinal plant, valued for its exceptional therapeutic properties. Studies has demonstrated that chamomile possesses a range of beneficial effects, including anti-inflammatory, antioxidant, antiseptic, analgesic, antimicrobial, antispasmodic, antiviral, and sedative properties. With the growing global interest in natural ingredients and healthier alternatives to synthetic compounds, there is an increasing demand for accessible and cost-effective bioactive sources. In this context, chamomile waste—such as flower powder or distillation byproducts—emerges as a promising source of bioactive compounds with diverse potential applications. This paper provides a comprehensive overview of the phytochemical composition and safety profile of chamomile waste, highlighting its rich content of polyphenols, polysaccharides, pyrrolizidine alkaloids, and other bioactive compounds. Safety considerations, with particular attention to the presence of pyrrolizidine alkaloids, are also addressed. In addition to discussing its phytochemical composition, the paper reviews various extraction techniques for isolating bioactive compounds from chamomile waste. Traditional solvent extraction methods, including Soxhlet extraction, are compared to green extraction techniques such as supercritical fluid extraction, ultrasound-assisted extraction, and microwave-assisted extraction. Furthermore, the potential applications of chamomile waste in the pharmaceutical, cosmetic, and food industries are explored. Due to its bioactive properties, chamomile waste holds significant promise as an ingredient for skincare products, dietary supplements, and functional foods. The versatility of chamomile waste as a natural source of bioactive compounds presents opportunities for innovative product development and sustainable waste management practices.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103468"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Lactobacilli strains from Algerian traditional dairy products for probiotic potential benefits","authors":"Malika Bouchibane ,&nbsp;Nabil Touzout ,&nbsp;Djamel Ait Saada ,&nbsp;Abdelkader Elamine Dahou ,&nbsp;Farida Boumediene ,&nbsp;Djahira Hamed ,&nbsp;Selma Toumi ,&nbsp;Abderrahim Cheriguene","doi":"10.1016/j.bcab.2024.103472","DOIUrl":"10.1016/j.bcab.2024.103472","url":null,"abstract":"<div><div>This study explores the probiotic potential of five lactic acid bacteria species (LAB) isolated from traditional Algerian dairy products, with the goal of advancing the development of customized probiotics for innovative applications within the food industry. Tailoring probiotics for specific uses represents a significant advancement in this field. The focus is on five species: <em>Limosilactobacillus fermentum, Lactiplantibacillus pentosus, Lactiplantibacillus plantarum, Lacticaseibacillus paracasei</em> ssp <em>paracasei,</em> and <em>Lactiplantibacillus paraplantarum</em>, all identified through MALDI-TOF mass spectrometry, with confirmation via PCR and 16S rRNA sequencing. The probiotic potential of these strains was evaluated through <em>in vitro</em> experiments assessing tolerance to bile salts and acidity, hydrophobicity, hemolytic activity, sugar fermentation profiles, and resistance to antibiotics and phenols. Results indicated that all the strains displayed significant survival rates at pH 3, ranging from 77.18% ± 1.74–93% ± 1.95. Bile salt tolerance varied among strains, with survival rates between 60.77% ± 2.10 and 91.46% ± 1.68 at a 0.3% concentration, and between 53.91% ± 2.39 and 87.02% ± 2.57 at a 0.5% concentration. Hydrophobicity tests demonstrated moderate adhesion capabilities. Notably, only three strains exhibited resistance to 0.4% phenols, with growth percentages between 29.60% ± 2.03 and 45.97% ± 5.49. Antibiotic susceptibility tests revealed a high sensitivity to various antibiotics, while resistance was noted for cefoxidim, tetracycline, and vancomycin, with the exception of <em>L. fermentum</em>, which remained sensitive to vancomycin. All tested strains were non-hemolytic and capable of fermenting a majority of sugars. Overall, the isolated strains showcase promising probiotic characteristics, providing opportunities for innovation in the food sector by developing new fermented dairy products that meet consumer demands for health and naturalness.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103472"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of avocado oil: Insights into obesity metabolism and immune regulation through in vitro models","authors":"Manuela Machado,&nbsp;Eduardo M. Costa,&nbsp;Sara Silva,&nbsp;Ana Maria Gomes,&nbsp;Manuela Pintado","doi":"10.1016/j.bcab.2024.103464","DOIUrl":"10.1016/j.bcab.2024.103464","url":null,"abstract":"<div><div>Avocado oil boasts a compelling nutritional profile characterized by a richness of unsaturated fatty acids and key lipophilic compounds. This study aimed to explore the potential of avocado oil in obesity-related metabolic processes and immune responses modulation in <em>in vitro</em> cellular models. Results obtained unveiled avocado oil capacity to effectively regulate triglyceride accumulation in differentiated 3T3-L1 adipocytes, showcasing a remarkable five-fold increase in adipolysis rate at 15 mg/mL, and an increase in glucose uptake in insulin-stimulated cells. Furthermore, avocado oil exhibited significant immunomodulatory effects in IL-1β-stimulated Caco-2 cells, as it reduced IL-6 (by 11%) and IL-8 (by 12%) production at 10 mg/mL, while only a marginal uptick in TNF-α secretion was observed at the same concentration. These findings underscore the potential of avocado oil to be a beneficial component in functional foods and nutraceuticals targeted at obesity management.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103464"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological evaluation of 2-(3,4-dihydroxyphenyl)-4-((4-methoxyphenyl)imino)-4H-chromene-3,5,7-triol: Design, synthesis, and molecular docking studies","authors":"Udaya Rajesh R,&nbsp;Dhanaraj Sangeetha","doi":"10.1016/j.bcab.2024.103471","DOIUrl":"10.1016/j.bcab.2024.103471","url":null,"abstract":"<div><div>Plant materials are known to contain a category of naturally occurring compounds referred to as flavonoids. Quercetin is classified as a flavonol, which is present in various parts of plants and serves multiple functions within biological systems. This article outlines the synthesis of quercetin Schiff base (Qpa) through a condensation reaction involving quercetin and <em>p</em>-anisidine, followed by characterization using spectroscopic methods such as FTIR, UPLC, ¹H NMR, ¹³C NMR, elemental analysis, and HRMS. The biological applications of Qpa were investigated through antioxidant assays, anti-inflammatory tests, antibacterial evaluations, cytotoxicity assessments, and molecular docking studies. The results indicated that the ¹H NMR and ¹³C NMR spectra exhibited a distinct peak for OCH₃ at 3.66 and 55.73 ppm, respectively. In terms of biological activity, Qpa demonstrated significant antioxidant and anti-inflammatory effects at concentrations of 142.7 μg/mL ± 0.0015 and 247 μg/mL ± 0.0015, respectively. Additionally, Qpa exhibited antibacterial properties comparable to the positive control against both gram-positive and gram-negative bacteria at a concentration of 248 μg. Cytotoxicity studies revealed that Qpa had pronounced effects on MCF-7 and HepG2 cell lines. Furthermore, molecular docking analyses indicated that Qpa displayed high binding energy with AKT, tRNA synthetase, and TNF-α. Future <em>in vivo</em> studies are recommended to elucidate the specific mechanisms of action and to achieve significant results in the pursuit of a novel therapeutic agent.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103471"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UV/EMS mutagenized thermo-alkalophilic polygalacturonase from Glutamicibacter sp.: Physicochemical insights and biotechnological prospects","authors":"Mary Arpana,&nbsp;Aneesa Fasim,&nbsp;Sunil S. More","doi":"10.1016/j.bcab.2024.103465","DOIUrl":"10.1016/j.bcab.2024.103465","url":null,"abstract":"<div><div>The current study discusses the physicochemical properties of an alkalophilic polygalacturonase (PGmut) that was extracted from the UV/EMS mutagenized <em>Glutamicibacter</em> sp. MAIDO R22b-13 strain in comparison to its wildtype (PGwt). Further, its applications in various biotechnology sectors were also assessed. The PGwt and PGmut enzymes were purified by gel permeation chromatography and biochemical analysis determined them to be alkalophilic with pH optima of 10, however mutagenesis enhanced the temperature optima from 37 °C (PGwt) to 50 °C. The molecular weight of both enzymes was confirmed to be 48.67 kDa by SDS-PAGE and LC-MS. PGmut showed higher substrate affinity and velocity, with Km and Vmax of 0.051 mg/ml and 300.12 μmol/min, respectively, compared to 0.072 mg/ml and 205.30 μmol/min for PGwt. Co²⁺ and Mn²⁺ ions enhanced PGmut activity, while Ag²⁺, Cu²⁺, Hg²⁺, and EDTA lowered it. Nevertheless, dialysis-based EDTA removal moderately restored enzyme activity, confirming it to be a metalloenzyme. In addition, PGmut enhanced olive oil production, enabled lycopene extraction from tomato peels, and effectively softened sugarcane bagasse pulp for papermaking. Therefore, this study highlights PGmut's enhanced properties that offers cost-efficient, sustainable, time-saving bioprocess solution, benefiting industry and the environment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103465"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}