Biocatalysis and agricultural biotechnology最新文献

{"title":"Harnessing Bacillus safensis as biofertilizer for sustainable drought alleviation in Brassica juncea L","authors":"","doi":"10.1016/j.bcab.2024.103388","DOIUrl":"10.1016/j.bcab.2024.103388","url":null,"abstract":"<div><div>Carrier-based biofertilizers, which involve the introduction of plant growth-promoting bacteria into agricultural industry waste materials, are gaining increasing attention due to their profoundly positive impacts on soil health. In current study, various carriers, including dry leaves, sugarcane husk (SCH), rice husk (RH), and a combination/Mixture (MIX) of all three, were employed to support the bacterial strain, <em>Bacillus safensis</em> (SCAL1). This strain was utilized as a bioinoculant in the production of carrier-based biofertilizers. This particular strain was sourced from the Environmental and Microbial Botany Lab at Quaid-i-Azam University in Islamabad. <em>Bacillus safensis</em> strain exhibited notable improvements, evident in a high rate of longevity and larger colony-forming units within the carriers, as confirmed by measurements taken after 21 days of incubation. The drought stress was induced using polyethylene glycol solutions with concentrations of 15% and 25% administrated 6 and 15 days after germination, respectively. Four distinct biofertilizer types were formulated in the study: DL + SCAL1, RH + SCAL1, SCH + SCAL1, and MIX + SCAL1. When applied to <em>Brassica juncea</em> L. plants, all of these biofertilizers demonstrated the ability to mitigate drought stress and enhance the quality of <em>Brassica juncea</em> L. The immobilization onto SCAL1 by using SCH showed the best result compared to the liquid inoculum as carrier materials improve stability and shelf life. Furthermore, our results affirmed that carrier-based biofertilizers improved biochemical and physiological attributes of plants during drought conditions, highlighting their importance as a valuable resource for mitigating drought stress in <em>Brassica juncea</em> L. The findings revealed that carrier-based biofertilizers are an excellent source of diverse phytohormones, which are pivotal in alleviating drought stress.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro-nano bubbles in action: AC/TiO2 hybrid photocatalysts for efficient organic pollutant degradation and antibacterial activity","authors":"","doi":"10.1016/j.bcab.2024.103400","DOIUrl":"10.1016/j.bcab.2024.103400","url":null,"abstract":"<div><div>Developing highly active and sustainable photocatalysts is crucial for environmental remediation. This work focuses on the enhanced photocatalytic degradation efficiency of synthetic dyes using TiO₂-coated AC hybrid photocatalysts under MNB aeration. The TiO₂-coated AC hybrid photocatalyst (HP) was synthesized via a sol-gel method and characterized using XRD, FTIR, SEM, EDS, HR-TEM, and DRS. The study investigated the photocatalytic degradation of three dyes indigo carmine (IC), reactive black 5 (RB5), and methylene blue (MB) in wastewater, with initial dye concentrations ranging from 10 to 100 μM. Under UVA irradiation, the hybrid photocatalyst with MNB aeration (HP + UVA + MNB) achieved the highest degradation efficiencies of 69.09%, 60.06%, and 55.19% for IC, MB, and RB5, respectively. Further analysis showed that the chromophores and complex structures of these dyes were broken into intermediate products. The Langmuir-Hinshelwood kinetic model was used to describe the reaction mechanisms. The HP + UVA + MNB system demonstrated superior photocatalytic degradation activity, making it suitable for operational and environmental applications in dye wastewater treatment. Additionally, the antibacterial properties of AC and HP were tested at 100 μg/mL, showing effectiveness against both Gram-positive <em>Staphylococcus aureus</em> and Gram-negative <em>Escherichia coli.</em></div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of agricultural by-product for cleaner and sustainable production of microbial pigments and cellulolytic enzymes in a zero-waste approach","authors":"","doi":"10.1016/j.bcab.2024.103397","DOIUrl":"10.1016/j.bcab.2024.103397","url":null,"abstract":"<div><div>This work presents a zero-waste concept for the valorization of banana peel into <em>Monascus</em> value-added metabolites, such as pigments and cellulolytic enzymes, and for evaluation of the feasibility of treated peel as a promising source for animal feedstuff. <em>M. purpureus</em> YRU01 exhibited satisfactory pigment and cellulolytic enzyme production in solid-state fermentation. Particularly, repeated solid-state fermentation of non-sterile peel with 90% substrate replacement was effective in offering high recovery of pigments, xylanase, and cellulase (7.19-, 31.19-, and 92.62-fold higher than those of solid-state fermentation, respectively). The LC/MS profile of fermented peel provided important evidence of fungal metabolites (&gt;100 metabolites) for understanding and evaluating the fermentation. Moreover, citrinin-related mycotoxin fragments were not found. The treated peel had high carbohydrate, neutral detergent fiber, and acid detergent fiber contents, but low protein content, indicating its potential to be used as a roughage in animal feed.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and optimization of biofuels from locally isolated algal biomass: Strategies for circular economy integration","authors":"","doi":"10.1016/j.bcab.2024.103383","DOIUrl":"10.1016/j.bcab.2024.103383","url":null,"abstract":"<div><div>In the present study, we investigated the potential of locally isolated algal strains as alternative energy sources for sustainable biofuel production. The focus of this study was to identify algal strains that are capable of accumulating oils rich in essential fatty acids. Algal samples were collected from different areas and 14 isolates were obtained. Among the various pretreatment methods tested, hydrothermal pretreatment using sulfuric acid at 95 °C yielded the best results, with sample IIB-14 containing more than 2% reducing sugars. These sugars were then used for fermentation with the <em>S. cerevisiae</em> strain, resulting in an ethanol concentration of 3.52% ± 0.2%. This holistic approach contributes to the development of low-cost and environmentally friendly alternatives to traditional energy sources. While algal biofuels offer a promising substitute for fossil fuels, further advancements are needed before they can be widely adopted in the fuel market. Among these, isolates IIB-8 and IIB-9 showed the highest oil yields of 22.84% and 24.69% (w/w), respectively. The specific environmental settings for optimal growth of these strains were determined, and the physicochemical parameters of the oils, including iodine value, viscosity, density, acid value, saponification value, unsaponifiable mass, and peroxide value, were analyzed. The transesterification of oils into fatty acid methyl esters (FAMEs) revealed the presence of significant amounts of fatty acids, including EPA, DHA, and linoleic acid. Moreover, the study also explored the potential of algal biomass for bioethanol production, addressing the sustainability concerns of renewable energy supplies. Hydrothermal pretreatment using sulfuric acid at 95 °C yielded the highest concentration of reducing sugars (&gt;2%) in IIB-14. Sugar extracted from algal biomass was used for fermentation. The <em>Saccharomyces cerevisiae</em> strain used for the fermentation process yielded an ethanol concentration of 3.52% ± 0.2%. This holistic approach contributes to the development of low-cost and environment-friendly alternatives to renewable energy sources. Algal biofuels may offer a practical substitute for fossil fuels, but there is still a long way to go before they can enter the fuel market and are widely used.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasible biosynthesis of biologically active metabolites in in vitro culture of Macrotyloma uniflorum","authors":"","doi":"10.1016/j.bcab.2024.103374","DOIUrl":"10.1016/j.bcab.2024.103374","url":null,"abstract":"<div><div><em>Macrotyloma uniflorum,</em> commonly called “Horse gram” is an underutilized pulse crop recognized for its great nutritional significance and a broad range of biological properties. There have been no <em>in vitro</em> studies for the biosynthesis of enhanced bioactive metabolites in the callus culture of <em>M. uniflorum</em>. In the study reported here, we have designed a feasible protocol for high-frequency callus induction and maintenance utilizing a leaf as an explant grown on MS media enriched with various concentrations of different plant growth regulators (PGRs) including α-naphthalene acetic acid (NAA), 6-benzylaminopurine (BAP) and thidiazuron (TDZ) either alone or in combination. Among all the tested PGRs, NAA alone resulted in high-frequency callus induction (97%), and maximum biomass accumulation (Fresh weight (FW): 200 g/L: Dry weight (DW): 20.4 g/L). Moreover, hormonally optimized callus cultures exhibit maximum production of phenolic compounds (166.6 mg/L) and flavonoid compounds (351.6 mg/L). The antioxidant potential of calli extracts was also determined by utilizing various antioxidant activities. Maximum antioxidant activities ((2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) ABTS = 497.9; TEAC μM; (Ferric reducing antioxidant power) FRAP = 823 TEAC μM; (Cellular Antioxidant Activity) CAA: 58.2%) were recorded in leaf-derived calli supplemented with different PGRs treatments. High-performance liquid chromatography (HPLC) analysis further revealed maximum biosynthesis of caffeic acid (1.63 mg/g DW), gallic acid (8.92 mg/g DW), kaempferol (0.71 mg/g DW), myricetin (0.39 mg/g DW), apigenin (0.64 mg/g DW) at 10 mg/L BAP and isorhamnetin (0.68 mg/g DW) at 1 mg/L TDZ + 10 mg/L NAA. The objective of this study was to explore <em>in vitro</em> biosynthesis of enhanced bioactive metabolites in the callus culture of <em>M. uniflorum</em>, leveraging a feasible protocol for high-frequency callus induction and maintenance. The results showcase the remarkable efficacy of NAA in callus induction and biomass accumulation, highlighting the hormonally optimized callus cultures as a potent source for enhanced biosynthesis of bioactive metabolites, paving the way for further applications in pharmaceutical and commercial industries.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot synthesis of stable vitamin C analogue using trans-glycosylating enzyme from Aspergillus carbonarius BTCF 5","authors":"","doi":"10.1016/j.bcab.2024.103401","DOIUrl":"10.1016/j.bcab.2024.103401","url":null,"abstract":"<div><div>Biocatalysis using transglycosidases for one-pot glycosylation of small molecules is a greener alternative to chemical glycosylation. The transglycosylation potential of the crude culture filtrate of <em>Aspergillus carbonarius</em> BTCF 5 was evaluated based on the biotransformation of the target molecule, ascorbic acid (Vitamin-C), to its more stable glycosylated analogue -2-O-α-D-glucopyranosyl ascorbic acid (AA2G), using maltose as the glycosyl donor. Media engineering increased the transglycosylation yield by 64%, whereas the use of concentrated culture filtrate increased the transglycosylation yield by 70%. Protein purification studies identified the candidate transglycosylating enzyme to be a GH 31 family CAZyme (α-transglucosidase). A minimally purified enzyme could enhance the transglycosylation yield by 77% with a total AA2G yield of 91.5 mM (10.32 g/L) and improved the overall conversion rate of ascorbic acid and maltose in the reaction by 16% and 6.3% respectively from the base level. A two-step column chromatography helped in product purification with 46% recovery. The structural characterization of purified AA2G provided a detailed insight into the regioselectivity of the enzyme to glycosylate C2 of ascorbic acid and its ability to form AA2G as the predominant isomer, which is also the most preferred one for end applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant activity evaluation of the combination of oregano (Origanum vulgare) and laurel (Laurus nobilis) essential oil as two natural “chain-breaking” antioxidants by direct measurement of sunflower oil oxidation","authors":"","doi":"10.1016/j.bcab.2024.103403","DOIUrl":"10.1016/j.bcab.2024.103403","url":null,"abstract":"<div><div>Antioxidants are commonly used to reduce oxidative damage in lipids, and essential oils offer natural alternatives with antioxidant properties. This research seeks to determine the antioxidant efficiency of the combination of laurel (laurus nobilis) and oregano (origanum vulgare) essential oils exhibiting a chain-breaking mechanism. To this end, an accelerated oxidation of sunflower vegetable oil was carried out at 60 °C for 28 days, in combination with concentrations of 0.05% w/w (1) and 0.02% w/w (2) of the essential oils. BHT at 0.02% w/w was used as a reference antioxidant. The chemical indicators of oxidation (dienes conjugated, peroxides, anisidine and TOTOX values) and volatiles (2-heptenal Z, 2,4-Decadienal EZ and EE) were determined. While no substantial differences were found between the studied concentrations of the essential oils, a decrease in the protective effect was observed when the concentration of laurel was increased. Interestingly, the antioxidant effect of both oregano and laurel essential oils was observed to be similar to that of BHT. The results suggest that the combination of these essential oils with chain-breaking antioxidant mechanisms might be used as an antioxidant in food.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of antifungal properties in medicinal plant extracts for sustainable agriculture","authors":"","doi":"10.1016/j.bcab.2024.103398","DOIUrl":"10.1016/j.bcab.2024.103398","url":null,"abstract":"<div><div>The escalating environmental repercussions of pesticide use in pest and disease management underscore the urgency for alternative strategies. One promising avenue involves harnessing natural antifungal properties present in plant extracts. This study aimed to assess the antifungal efficacy of aqueous and ethanolic extracts from five medicinal plants, viz., <em>C. sativa</em> L., <em>S. moorcraftiana</em> L., <em>S. nigrum</em> L., <em>F. vesca</em> L., and <em>R. pseudoacacia,</em> which were screened for their antifungal activity against various soil-borne fungi, viz., <em>A. niger</em>, <em>A. flavus</em>, <em>A</em>. <em>terreus</em>, <em>R. stolonifer, M. mucedo, F. oxysporum, A. alternata, P. notatum, C. cladosporioides,</em> and <em>C. lunata</em>. Phytochemical analysis exhibited the presence of phenols, alkaloids, tannins, flavonoids, quinines, and terpenoids. Results revealed potent antifungal activity across all tested fungi, significantly inhibiting mycelial growth compared to control. <em>S. nigrum</em> and <em>C. sativa</em> extracts exhibited the highest efficacy against multiple fungal pathogens. The inhibition in mycelial growth in ethanolic and aqueous plant extracts of <em>C. sativa</em> against <em>A. niger</em> varies between 3.00 mm to 6.00 mm and 4.00 mm–6.33 mm, against <em>A. flavus</em> 3.33 mm–11.66 mm and 4 mm–12.66 mm, <em>A. terreus</em> (3.00 mm–6.00 mm and 3.66 mm–7.00 mm), <em>R. stolonifer</em> (3.00 mm–5.33 and 3.66 mm–6.33 mm), <em>M. mucedo</em> (8.33 mm–17.66 mm and 9.66 mm–18.66 mm), <em>F. oxysporum</em> (6.66 mm–12.33 mm and 7.33 mm–13.33 mm), <em>A. alternata</em> (4.66 mm–11.33 mm and 5.33 mm–14.00 mm), <em>P. notatum</em> (9.33 mm–23.00 mm and 9.66 mm–24.33 mm), <em>C. cladosporioides</em> (4.33 mm–8.66 mm and 5.33 mm–9.66 mm), and <em>C. lunata</em> (14.00 mm–20.33 mm and 17.00 mm–21.33 mm) in different concentrations of plant extracts respectively. Likewise, the inhibition in mycelial growth in ethanolic and aqueous plant extracts of <em>S. nigrum</em> against <em>A. niger</em> at different concentrations varies between 8.00 mm to 17.66 mm and 9.00 mm–18.66 mm, <em>A. flavus</em> (8.33 mm–12.66 mm and 9 mm–14 mm), <em>A. terreus</em> (2.33 mm–5.66mm and 3 mm–7 mm), <em>R. stolonifer</em> (13.33 mm–20.33 mm and 17.66 mm–24.66 mm), <em>M. mucedo</em> (10.66 mm–12.00 mm and 11.66 mm–13.00 mm), <em>F. oxysporum</em> (15.66 mm–22.33 and 18.00 mm–24.00 mm), <em>A. alternata</em> (10.66 mm–13.00 mm and 11.66 mm–14.00 mm), <em>P. notatum</em> (5.33 mm–13.33 mm and 6.33 mm–18.66 mm, <em>C. cladosporioides</em> (3.33 mm–7.66 mm and 4.00 mm–8.00 mm), and <em>C. lunata</em> (3.66 mm–6.33 mm and 4.66 mm–6.66 mm), respectively. These findings suggest that these plant extracts have the potential to be natural fungicides, providing promising alternatives for disease management in agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine protease-producing bacteria as potential probionts for advancing mariculture","authors":"","doi":"10.1016/j.bcab.2024.103392","DOIUrl":"10.1016/j.bcab.2024.103392","url":null,"abstract":"<div><div>The research delves into the isolation and characterization of protease-producing bacteria from seawater, sediment, and the gastrointestinal tracts (GITs) of Snubnose pompano (<em>Trachinotus blochii</em>) for potential use as probiotics in marine finfish culture. Five bacterial strains screened from the marine environmental samples-<em>Chryseobacterium indologenes Chryseobacterium culicis, Bacillus aerius</em>, <em>Bacillus altitudinis</em>, and <em>Bacillus pumilus</em> were isolated and characterized both phenotypically and genotypically. Protease activity was assessed across acidic to alkaline pH levels (5–10) and temperatures (20 °C–120 °C) to identify the optimum conditions. <em>B. pumilus</em>, isolated from the GIT of <em>T. blochii</em>, exhibited the highest protease activity (0.088 Unit/ml) at pH 6 and 40 °C, making it a particularly potent strain. <em>B. altitudinis</em> and <em>B. aerius</em> also demonstrated significant protease activity at pH 7, with values of 0.086 Unit/ml and 0.081 Unit/ml, respectively. The <em>in vitro</em> study suggests that all three <em>Bacillus</em> strains, have the potential to serve as probiotics in marine finfish mariculture, emphasizing <em>B. pumilus</em> due to its superior protease activity under defined conditions. This research contributes to the development of tailored probiotics for improving protein digestibility in mariculture systems, advancing sustainable practices in marine finfish production.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing bioencapsulation of yeast cells by Aspergillus tubingensis TSIP9 and applications in bioethanol production through repeated-batch fermentation","authors":"","doi":"10.1016/j.bcab.2024.103377","DOIUrl":"10.1016/j.bcab.2024.103377","url":null,"abstract":"<div><div>This study aimed to immobilize yeast cells using filamentous fungi owing to a number of advantages including less chemical usage, spontaneous encapsulation, strong adhesion and biocompatibility. Filamentous fungus, <em>Aspergillus tubingensis</em> TSIP9 could form sphere-shape and packed pellets using either fungal fresh spores or those stored in liquid medium for 6 weeks at 4 °C. The fungus was used to immobilize yeast <em>Saccharomyces cerevisiae</em> FAI via adsorption and co-cultivation methods. Scanning electron microscopy images revealed that the bioencapsulated yeast cells via co-cultivation seemed to be more tightly adhered on fungal mycelia and surrounded by extracellular polymeric substances. The yeast biocapsules also exhibited higher stability and maintained their structural integrity during repeated-batch fermentation while the immobilized yeast cells by adsorption gradually degraded during their repeated uses. The bioethanol production from glucose by yeast biocapsules were in the range of 95–98 g/L with the bioethanol yield of 0.49–0.54 g-ethanol/g-consumed glucose. The yeast biocapsules could produce bioethanol well when using enzymatic hydrolysate of lignocellulosic palm waste, as alternative cheap carbon source, with the comparable bioethanol yield of 0.49 ± 0.17 g-ethanol/g-consumed glucose. The spontaneous and inter-species bioencapsulation show the perspectives as active biocatalysts with high cell retention for repeated uses.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}