Biocatalysis and agricultural biotechnology最新文献_第7页

Morphological, ultrastructural, biochemical and yield attributes variations in Nigella sativa L. by cadmium (Cd) stress: Implications for human health risk assessment

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103514

Adnan Khan , Athar Ali Khan , Sayma Samreen , Moh Sajid Ansari , Syed Aiman Hasan , Mohd Irfan

This study was conducted in the pots where each pot was filled with 4 kg soil that was contaminated with varying concentrations of cadmium (Cd) which are 1.5 mM, 2.0 mM, 2.5 mM, 3.0 mM, 3.5 mM, and 4.0 mM equal to 168.61, 224.82, 281, 337.23, 393.44 and 449.64 mg/L respectively), except the control group, each treatment was replicated thrice (n = 3) including control. We evaluated and examined growth, yield, metal stress tolerance, and metal buildup characteristics at the harvesting stage of black cumin (Nigella sativa L.) plants. The findings indicated that all levels of Cd had a substantial (p ≤ 0.05) negative impact on black cumin plants' growth and yield-related characteristics. Low (1.5 mM) to high doses (4.0 mM) of Cd significantly (p ≤ 0.05) reduced chlorophyll a (30%–72%), chlorophyll b (25%–69%) and total chlorophyll (56% to 82%) levels in the fresh leaf tissues, as well as significantly (p ≤ 0.05) decreased the fruit yield (12%–45%) and seed yield (39%–75%) of black cumin plants. Energy dispersive x-ray (EDX) analysis and scanning electron microscopy (SEM) of control and Cd-treated plant leaf samples showed significant Cd accumulation, and the ultrastructure of both revealed that Cd stress reduced the stomatal density and size of the stomatal aperture compared to the control. Bioaccumulation levels of Cd in the seeds of all treated plants are above the threshold limit (0.3 mg/kg) set by the World Health Organization (WHO) for medicinal plants and are unsafe for human consumption. Therefore, farmers must avoid cultivating black cumin crops in Cd polluted soil from economic and health perspectives.

{"title":"Morphological, ultrastructural, biochemical and yield attributes variations in Nigella sativa L. by cadmium (Cd) stress: Implications for human health risk assessment","authors":"Adnan Khan , Athar Ali Khan , Sayma Samreen , Moh Sajid Ansari , Syed Aiman Hasan , Mohd Irfan","doi":"10.1016/j.bcab.2025.103514","DOIUrl":"10.1016/j.bcab.2025.103514","url":null,"abstract":"<div><div>This study was conducted in the pots where each pot was filled with 4 kg soil that was contaminated with varying concentrations of cadmium (Cd) which are 1.5 mM, 2.0 mM, 2.5 mM, 3.0 mM, 3.5 mM, and 4.0 mM equal to 168.61, 224.82, 281, 337.23, 393.44 and 449.64 mg/L respectively), except the control group, each treatment was replicated thrice (n = 3) including control. We evaluated and examined growth, yield, metal stress tolerance, and metal buildup characteristics at the harvesting stage of black cumin (Nigella sativa L.) plants. The findings indicated that all levels of Cd had a substantial (p ≤ 0.05) negative impact on black cumin plants' growth and yield-related characteristics. Low (1.5 mM) to high doses (4.0 mM) of Cd significantly (<em>p</em> ≤ 0.05) reduced chlorophyll <em>a</em> (30%–72%), chlorophyll <em>b</em> (25%–69%) and total chlorophyll (56% to 82%) levels in the fresh leaf tissues, as well as significantly (<em>p</em> ≤ 0.05) decreased the fruit yield (12%–45%) and seed yield (39%–75%) of black cumin plants. Energy dispersive x-ray (EDX) analysis and scanning electron microscopy (SEM) of control and Cd-treated plant leaf samples showed significant Cd accumulation, and the ultrastructure of both revealed that Cd stress reduced the stomatal density and size of the stomatal aperture compared to the control. Bioaccumulation levels of Cd in the seeds of all treated plants are above the threshold limit (0.3 mg/kg) set by the World Health Organization (WHO) for medicinal plants and are unsafe for human consumption. Therefore, farmers must avoid cultivating black cumin crops in Cd polluted soil from economic and health perspectives.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103514"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143369765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification, and bioprospection of endophytic bacteria from medicinal plant Mikania glomerata (Spreng.) and the consortium of Pseudomonas as plant growth promoters

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103530

Bianca Rosini , Aline Maria Bulla , Julio Cesar Polonio , Andressa Domingos Polli , Angela Aparecida da Silva , Rodrigo Pawloski Schoffen , Verci Alves de Oliveira-Junior , Shalene da Silva Santos , Halison Correia Golias , João Lúcio Azevedo , João Alencar Pamphile

Brazil is the largest exporter of soybeans in the world, and it is of great socio-economic importance to the country. Plants, including soybeans, benefit significantly from endophytic bacteria, some of which are able to promote plant growth and stimulate cell development. In this study, we isolated endophytic bacteria from Mikania glomerata, a medicinal plant commonly known as ‘guaco,’ carried out molecular identification analyses, assessed their biological activities (inorganic phosphate solubilization, biological nitrogen fixation, indolic compounds, and siderophore production), and investigated the potential of the most promising isolates as growth promoters of soybean. Taxonomic analysis identified 31 endophytic bacteria, including Acidovorax, Acinetobacter, Bacillus, Chryseobacterium, Comamonas, Curtobacterium, Delftia, Kurthia, Microbacterium, Micrococcus, Pseudomonas, Serratia, Stenotrophomonas, and Streptomyces. Strains of Pseudomonas japonica and Pseudomonas tolaasii were tested for their ability to promote soybean growth under greenhouse conditions in association with a coating polymer for seed treatment. Plant height (43.67 cm), trifoliate leaf number (9.25), root length (108.4 cm), root fresh weights (15.7 g), root dry weights (1.06 g), shoot fresh weights (5.7 g) and shoot dry weights (1.39 g) were positively influenced by combined treatment of soybean seeds with two endophytic isolates and the polymer, compared to the control group (35 cm, 8 leaves, 91.42 cm, 10.93 g, 0.70 g, 3.41 g, and 0.87 g, respectively), with only the shoot dry weight showing statistical significance. This results in improved seedling development and plant growth, indicating a promising option for reducing chemical fertilizers.

{"title":"Isolation, identification, and bioprospection of endophytic bacteria from medicinal plant Mikania glomerata (Spreng.) and the consortium of Pseudomonas as plant growth promoters","authors":"Bianca Rosini , Aline Maria Bulla , Julio Cesar Polonio , Andressa Domingos Polli , Angela Aparecida da Silva , Rodrigo Pawloski Schoffen , Verci Alves de Oliveira-Junior , Shalene da Silva Santos , Halison Correia Golias , João Lúcio Azevedo , João Alencar Pamphile","doi":"10.1016/j.bcab.2025.103530","DOIUrl":"10.1016/j.bcab.2025.103530","url":null,"abstract":"<div><div>Brazil is the largest exporter of soybeans in the world, and it is of great socio-economic importance to the country. Plants, including soybeans, benefit significantly from endophytic bacteria, some of which are able to promote plant growth and stimulate cell development. In this study, we isolated endophytic bacteria from <em>Mikania glomerata</em>, a medicinal plant commonly known as ‘guaco,’ carried out molecular identification analyses, assessed their biological activities (inorganic phosphate solubilization, biological nitrogen fixation, indolic compounds, and siderophore production), and investigated the potential of the most promising isolates as growth promoters of soybean. Taxonomic analysis identified 31 endophytic bacteria, including <em>Acidovorax</em>, <em>Acinetobacter</em>, <em>Bacillus</em>, <em>Chryseobacterium</em>, <em>Comamonas</em>, <em>Curtobacterium</em>, <em>Delftia</em>, <em>Kurthia</em>, <em>Microbacterium</em>, <em>Micrococcus</em>, <em>Pseudomonas</em>, <em>Serratia</em>, <em>Stenotrophomonas</em>, and <em>Streptomyces</em>. Strains of <em>Pseudomonas japonica</em> and <em>Pseudomonas tolaasii</em> were tested for their ability to promote soybean growth under greenhouse conditions in association with a coating polymer for seed treatment. Plant height (43.67 cm), trifoliate leaf number (9.25), root length (108.4 cm), root fresh weights (15.7 g), root dry weights (1.06 g), shoot fresh weights (5.7 g) and shoot dry weights (1.39 g) were positively influenced by combined treatment of soybean seeds with two endophytic isolates and the polymer, compared to the control group (35 cm, 8 leaves, 91.42 cm, 10.93 g, 0.70 g, 3.41 g, and 0.87 g, respectively), with only the shoot dry weight showing statistical significance. This results in improved seedling development and plant growth, indicating a promising option for reducing chemical fertilizers.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103530"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms underlying the decolorization of indigo carmine and coomassie blue R-250 by Streptomyces salinarius CS29 laccase

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103513

Kamonpan Sanachai , Bodee Nutho , Rakrudee Sarnthima , Wiyada Mongkolthanaruk , Jirada Pluemjai , Methus Kittika , Saranyu Khammuang

Laccase, a multicopper oxidase enzyme, possesses broad substrate specificity, enabling the oxidation of a diverse array of compounds. Among various microbial sources, Streptomyces species are prominent for producing stable and highly efficient laccases. This study investigated the decolorization potential of crude laccase extracted from Streptomyces salinarius CS29, specifically targeting indigo carmine and Coomassie Blue R-250 (CBBR). Optimal decolorization of both dyes was achieved within a pH range of 3–3.5, with pH 3.5 selected for subsequent experiments. Indigo carmine, at a concentration of 100 μM, demonstrated superior decolorization efficiency, reaching approximately 90% within 100 min. In contrast, decolorization of CBBR was less efficient. At concentrations of 50, 100, and 250 μM, approximately 50% decolorization was observed after 180 min. These findings suggest that laccase from S. salinarius CS29 exhibits greater efficacy in decolorizing indigo carmine compared to CBBR. Additionally, molecular docking and molecular dynamics (MD) simulations were employed to investigate the structural dynamics of the enzyme-dye complexes. MD simulations revealed that both indigo carmine and CBBR bind within the active site of the enzyme, predominantly through van der Waals interactions. Furthermore, key binding residues crucial for these interactions were identified. The findings of this study offer a foundational understanding that could significantly contribute to the development of environmentally sustainable strategies for the detoxification of dye-contaminated wastewater.

{"title":"Molecular mechanisms underlying the decolorization of indigo carmine and coomassie blue R-250 by Streptomyces salinarius CS29 laccase","authors":"Kamonpan Sanachai , Bodee Nutho , Rakrudee Sarnthima , Wiyada Mongkolthanaruk , Jirada Pluemjai , Methus Kittika , Saranyu Khammuang","doi":"10.1016/j.bcab.2025.103513","DOIUrl":"10.1016/j.bcab.2025.103513","url":null,"abstract":"<div><div>Laccase, a multicopper oxidase enzyme, possesses broad substrate specificity, enabling the oxidation of a diverse array of compounds. Among various microbial sources, <em>Streptomyces</em> species are prominent for producing stable and highly efficient laccases. This study investigated the decolorization potential of crude laccase extracted from <em>Streptomyces salinarius</em> CS29, specifically targeting indigo carmine and Coomassie Blue R-250 (CBBR). Optimal decolorization of both dyes was achieved within a pH range of 3–3.5, with pH 3.5 selected for subsequent experiments. Indigo carmine, at a concentration of 100 μM, demonstrated superior decolorization efficiency, reaching approximately 90% within 100 min. In contrast, decolorization of CBBR was less efficient. At concentrations of 50, 100, and 250 μM, approximately 50% decolorization was observed after 180 min. These findings suggest that laccase from <em>S. salinarius</em> CS29 exhibits greater efficacy in decolorizing indigo carmine compared to CBBR. Additionally, molecular docking and molecular dynamics (MD) simulations were employed to investigate the structural dynamics of the enzyme-dye complexes. MD simulations revealed that both indigo carmine and CBBR bind within the active site of the enzyme, predominantly through van der Waals interactions. Furthermore, key binding residues crucial for these interactions were identified. The findings of this study offer a foundational understanding that could significantly contribute to the development of environmentally sustainable strategies for the detoxification of dye-contaminated wastewater.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103513"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143204541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of bacterial cellulose from banana waste (Musa paradisiaca): A sustainable material for making cardboard paper

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103484

Juan Camilo Martinez , Lina Davila , Robin Zuluaga , Armando Ricardo , Jorge Figueroa , Maria Goreti de Almeida Oliveira , Yaremis Meriño-Cabrera

Bacterial cellulose (BC) is a biopolymer with advantages over plant cellulose, but its high cost has led to the search for alternative substrates. In Colombia, banana processing generates organic waste that can be utilized for BC production. Thus, the aim of this study was to assess the feasibility of using banana pulp waste (Musa paradisiaca) as an alternative carbon source in culture media for BC production by the bacteria K. xylinus and analyze its physicochemical properties and applicability in cardboard manufacturing. It was found that the 1:2 ratio of banana pulp to water yielded the highest BC production, suggesting greater nutrient availability in this ratio. A pH of 4.4 proved optimal for BC production, providing an appropriate acidic environment for bacterial growth. After optimizing the culture medium, BC yield increased significantly, with the production reaching up to 30 g/L, demonstrating the effectiveness of optimizing the substrate ratio and pH in enhancing cellulose synthesis. Physicochemical tests confirmed the purity of the obtained BC, with structural characteristics consistent with expected cellulose. Furthermore, cardboard made with this BC exhibited superior mechanical properties, with greater tensile and compression strength compared to the control cardboard, indicating enhanced durability and strength for industrial use. In conclusion, the use of banana waste as a carbon source for BC production offers a promising solution with significant economic and environmental benefits. The optimization of cultivation conditions, such as ratio and pH, and the confirmation of final product purity and quality, underscore the potential of this approach for sustainable industrial applications in biomaterial production.

{"title":"Production of bacterial cellulose from banana waste (Musa paradisiaca): A sustainable material for making cardboard paper","authors":"Juan Camilo Martinez , Lina Davila , Robin Zuluaga , Armando Ricardo , Jorge Figueroa , Maria Goreti de Almeida Oliveira , Yaremis Meriño-Cabrera","doi":"10.1016/j.bcab.2024.103484","DOIUrl":"10.1016/j.bcab.2024.103484","url":null,"abstract":"<div><div>Bacterial cellulose (BC) is a biopolymer with advantages over plant cellulose, but its high cost has led to the search for alternative substrates. In Colombia, banana processing generates organic waste that can be utilized for BC production. Thus, the aim of this study was to assess the feasibility of using banana pulp waste (<em>Musa paradisiaca</em>) as an alternative carbon source in culture media for BC production by the bacteria <em>K. xylinus</em> and analyze its physicochemical properties and applicability in cardboard manufacturing. It was found that the 1:2 ratio of banana pulp to water yielded the highest BC production, suggesting greater nutrient availability in this ratio. A pH of 4.4 proved optimal for BC production, providing an appropriate acidic environment for bacterial growth. After optimizing the culture medium, BC yield increased significantly, with the production reaching up to 30 g/L, demonstrating the effectiveness of optimizing the substrate ratio and pH in enhancing cellulose synthesis. Physicochemical tests confirmed the purity of the obtained BC, with structural characteristics consistent with expected cellulose. Furthermore, cardboard made with this BC exhibited superior mechanical properties, with greater tensile and compression strength compared to the control cardboard, indicating enhanced durability and strength for industrial use. In conclusion, the use of banana waste as a carbon source for BC production offers a promising solution with significant economic and environmental benefits. The optimization of cultivation conditions, such as ratio and pH, and the confirmation of final product purity and quality, underscore the potential of this approach for sustainable industrial applications in biomaterial production.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103484"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytohormone delivered through GRAS nano-platform promoting plant root growth: A promising strategy towards sustainable agricultural practices

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103474

Edwin Davidson , Jacqueline Tejada , Giulio Diracca , Preeti Maiti , Swadeshmukul Santra

Global food security will impose a major challenge in the years to come. The use of nanoscale delivery platforms in the agricultural sector holds the promise to provide a more sustainable alternative compared to conventional delivery of agrochemicals. Therefore, there is an imperative need to develop robust and cost-effective nanoscale delivery platforms. This work reports a nanodelivery system using tannic acid and polyvinylpyrrolidone (TA-PVP), GRAS ingredients, as a crop management tool to deliver plant growth regulators (PGR) for sustainable agriculture. Particularly, indole-3-acetic acid (IAA), a member of the auxin class of phytohormone due to the high hydrophobicity and degradability that limited its commercialization. The IAA nanoparticles (IAA NP) showed enhanced chemical stability in solution and sustained release, described by the Ritger and Korsmeyer–Peppas models. The non-phytotoxicity properties were studied with tomato seedlings up to 2500 ppm of IAA and the in vitro non-cytotoxicity was demonstrated with macrophages (J774A.1) and dermal fibroblasts (HDF) at the concentration below 39 ppm of IAA. Root growth and plant health assessments indicated enhanced root length and no detrimental impact on photosynthesis at 1 ppm IAA. Furthermore, the phosphomonoesterase activity was enhanced boosting the phosphorus metabolism. This study supports nano-enabled PGR delivery for sustainable agriculture.

{"title":"Phytohormone delivered through GRAS nano-platform promoting plant root growth: A promising strategy towards sustainable agricultural practices","authors":"Edwin Davidson , Jacqueline Tejada , Giulio Diracca , Preeti Maiti , Swadeshmukul Santra","doi":"10.1016/j.bcab.2024.103474","DOIUrl":"10.1016/j.bcab.2024.103474","url":null,"abstract":"<div><div>Global food security will impose a major challenge in the years to come. The use of nanoscale delivery platforms in the agricultural sector holds the promise to provide a more sustainable alternative compared to conventional delivery of agrochemicals. Therefore, there is an imperative need to develop robust and cost-effective nanoscale delivery platforms. This work reports a nanodelivery system using tannic acid and polyvinylpyrrolidone (TA-PVP), GRAS ingredients, as a crop management tool to deliver plant growth regulators (PGR) for sustainable agriculture. Particularly, indole-3-acetic acid (IAA), a member of the auxin class of phytohormone due to the high hydrophobicity and degradability that limited its commercialization. The IAA nanoparticles (IAA NP) showed enhanced chemical stability in solution and sustained release, described by the Ritger and Korsmeyer–Peppas models. The non-phytotoxicity properties were studied with tomato seedlings up to 2500 ppm of IAA and the in vitro non-cytotoxicity was demonstrated with macrophages (J774A.1) and dermal fibroblasts (HDF) at the concentration below 39 ppm of IAA. Root growth and plant health assessments indicated enhanced root length and no detrimental impact on photosynthesis at 1 ppm IAA. Furthermore, the phosphomonoesterase activity was enhanced boosting the phosphorus metabolism. This study supports nano-enabled PGR delivery for sustainable agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103474"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Technological advancements for the management of chromium: An unavoidable pollutant for plants 铬管理的技术进步：植物不可避免的污染物

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103466

Anuj Choudhary , Harmanjot Kaur , Saurabh Awasthi , Monika Koul , Sahil Mehta , Azamal Husen

Chromium (Cr) is a naturally occurring metal and an emerging heavy metal pollutant. With a surge in agricultural activities and industrial revolution, Cr accumulation is rising incrementally in the environment posing serious threats to all living entities. The toxicity and mobility of Cr (VI) is more compared to Cr (III) in living systems. Cr toxicity in soil and its further uptake in the plants affects the plants at pre-reproductive and post-reproductive stages and is manifested through changes in photosynthesis, seed formation, and seed germination primarily. High Cr levels in soil disturb the soil ecosystem further affecting water and nutrient uptake, which consequently affects many other physiological processes. The present paper is a comprehensive account of Cr toxicity in plants and highlights the most prevalent Cr remediation strategies based on physical, chemical, and biological methods. The use of novel methods using membrane systems like ultrafiltration, nanofiltration, reverse osmosis, etc. is increasingly being used in Cr remediation. The efficacy and efficiency of methods have been further evaluated. Contemporary research on Cr toxicity, the role of Cr in both plants and soil, and its ecological and environmental consequences on the environment have also been elucidated. However, with the advent of modern tools and techniques, Cr remediation methods have been modified. Research in the field of nanotechnology and information technology and further advancements in mitigating Cr remediation will be witnessed in the future.

{"title":"Technological advancements for the management of chromium: An unavoidable pollutant for plants","authors":"Anuj Choudhary , Harmanjot Kaur , Saurabh Awasthi , Monika Koul , Sahil Mehta , Azamal Husen","doi":"10.1016/j.bcab.2024.103466","DOIUrl":"10.1016/j.bcab.2024.103466","url":null,"abstract":"<div><div>Chromium (Cr) is a naturally occurring metal and an emerging heavy metal pollutant. With a surge in agricultural activities and industrial revolution, Cr accumulation is rising incrementally in the environment posing serious threats to all living entities. The toxicity and mobility of Cr (VI) is more compared to Cr (III) in living systems. Cr toxicity in soil and its further uptake in the plants affects the plants at pre-reproductive and post-reproductive stages and is manifested through changes in photosynthesis, seed formation, and seed germination primarily. High Cr levels in soil disturb the soil ecosystem further affecting water and nutrient uptake, which consequently affects many other physiological processes. The present paper is a comprehensive account of Cr toxicity in plants and highlights the most prevalent Cr remediation strategies based on physical, chemical, and biological methods. The use of novel methods using membrane systems like ultrafiltration, nanofiltration, reverse osmosis, etc. is increasingly being used in Cr remediation. The efficacy and efficiency of methods have been further evaluated. Contemporary research on Cr toxicity, the role of Cr in both plants and soil, and its ecological and environmental consequences on the environment have also been elucidated. However, with the advent of modern tools and techniques, Cr remediation methods have been modified. Research in the field of nanotechnology and information technology and further advancements in mitigating Cr remediation will be witnessed in the future.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103466"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal exergy analysis in fed-batch enzymatic hydrolysis: Assessing irreversibilities and sustainability in lignocellulosic biomass conversion

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103470

Suzimara Reis Silva , Jaqueline Alves Roberto , Tomaz Aprigio Silva , Esly Ferreira da Costa Jr. , Andréa Oliveira Souza da Costa

Although not yet widely industrialized, biofuels from lignocellulosic biomass show promise in enhancing industrial sustainability, with potential for a reduced carbon footprint and alignment with renewable energy goals. In this context, enzymatic hydrolysis assumes a fundamental position, being essential to make these biofuels viable. This study investigates the performance and thermodynamic sustainability of enzymatic hydrolysis in a fed-batch bioreactor, employing exergy analysis. The total exergy potential available to the system was 1239.93 kJ, with 30% of this exergy being destroyed at the end of the operation. The system achieved an overall exergy efficiency of 70.04%, while the rational efficiency reached 37.33% at the end of the process. Furthermore, the process sustainability indicator (PSI) reached a value of 1.67 and the system achieved a value for thermodynamic sustainability of 3.34. This study provides insights into the optimization of enzymatic hydrolysis processes, focusing on the reduction of irreversibilities, the improvement of exergy efficiency, and the enhancement of sustainability.

{"title":"Temporal exergy analysis in fed-batch enzymatic hydrolysis: Assessing irreversibilities and sustainability in lignocellulosic biomass conversion","authors":"Suzimara Reis Silva , Jaqueline Alves Roberto , Tomaz Aprigio Silva , Esly Ferreira da Costa Jr. , Andréa Oliveira Souza da Costa","doi":"10.1016/j.bcab.2024.103470","DOIUrl":"10.1016/j.bcab.2024.103470","url":null,"abstract":"<div><div>Although not yet widely industrialized, biofuels from lignocellulosic biomass show promise in enhancing industrial sustainability, with potential for a reduced carbon footprint and alignment with renewable energy goals. In this context, enzymatic hydrolysis assumes a fundamental position, being essential to make these biofuels viable. This study investigates the performance and thermodynamic sustainability of enzymatic hydrolysis in a fed-batch bioreactor, employing exergy analysis. The total exergy potential available to the system was 1239.93 kJ, with 30% of this exergy being destroyed at the end of the operation. The system achieved an overall exergy efficiency of 70.04%, while the rational efficiency reached 37.33% at the end of the process. Furthermore, the process sustainability indicator (PSI) reached a value of 1.67 and the system achieved a value for thermodynamic sustainability of 3.34. This study provides insights into the optimization of enzymatic hydrolysis processes, focusing on the reduction of irreversibilities, the improvement of exergy efficiency, and the enhancement of sustainability.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103470"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Filamentous fungi as a biotechnological tool for the biotransformation of betulin

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103476

Feyisayo O. Adepoju , Tarek M. Itani , Elena G. Kovaleva

Betulin, an inexpensive substrate extracted primarily from birch bark, exhibits wound-healing properties; yet its high hydrophobicity limits its bioavailability. Given that biotransformation can produce metabolites with modified chemical and biological properties, we sought to develop an efficient biocatalyst for the biotransformation of betulin. We isolated, characterized, and evaluated the ability of six fungi to biotransform betulin. The isolates were identified as Penicillium citreonigrum, Fusarium oxysporum, Fusarium redolens, Blastobotrys sp., Aspergillus niger, and Penicillium pimiteouiense and were cultured in biotransformation medium supplemented with betulin (10 mg/mL) for 4–7 days. Under the biotransformation conditions, Aspergillus niger exhibited the highest metabolic activity, producing biomass up to 1.29 g/L, acidifying the medium by increasing redox potential (404 mV), and lowering pH (3.29), while Blastobotrys sp. and Penicillium pimiteouiense had the lowest metabolic activity. All six isolates converted betulin to betulonic acid with Fusarium oxysporum exhibiting the highest bioconversion (0.74 g/L), followed by Fusarium redolens (0.72 g/L). Furthermore, at the adopted concentration, betulin biotransformation exerts no toxicity on fungal biomass. Therefore, the obtained results imply that the application of fungal cells as biological systems for betulin biotransformation can produce betulonic acid and for the first time, fungal-mediated biotransformation of betulin to betulonic acid was demonstrated.

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引用次数: 0

Corrigendum to “Comparative study on the production and immobilization of stem bromelain using polyacrylamide gel matrix and arginine coupled magnetic nanoparticles for enhanced operational stability” [Biocatalys. Agricult. Biotech. 62 (2024) 103451]

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103467

Fariha Javaid , Madeeha Shahzad Lodhi , Muhammad Tahir Khan , Muhammad Sarwar , Zahoor Qadir Samra

引用次数: 0

Microbial biofertilizers and biopesticides from vermicompost tea and rhizosphere of organic soilless melon crop: In vitro assessment

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103453

P.A. Mejía-Guerra , M.C. Salas-Sanjuán , M.R. Martínez-Gallardo , M.M. Jurado , M.J. Estrella-González , J.A. López-González , F. Suárez-Estrella , M.J. López

Microorganisms offer an environmentally sustainable alternative to synthetic pesticides and fertilizers. This study focuses on the identification and selection of microorganisms from vermicompost tea and the rhizosphere of an organic soilless melon crop, with potential for use as biopesticides and biofertilizers in agriculture. The biofertilizing capabilities of these microorganisms, such as nitrogen fixation, phosphate solubilization, and siderophore production, were evaluated. As part of the biopesticide assessment, their in vitro antagonistic effects against eight bacterial and fungal plant pathogens were tested. Additionally, a predictive study on the production of secondary metabolites of agronomic interest was conducted for the selected strains. The bacteria Acinetobacter beijerinckii B9, Kocuria palustris B23, Pantoea agglomerans B32, Rahnella aquatilis B21, Streptomyces griseorubiginosus B29 and Bacillus licheniformis B15, as well as the fungi Aspergillus fumigatus H4, Aspergillus niger H6 and Aspergillus terreus H2 showed excellent biopesticidal and biofertilizer activities. Among these, the selected fungi and the filamentous bacterium S. griseorubiginosus B29 were particularly notable for producing many secondary metabolites associated with antimicrobial activity and the induction of plant defense mechanisms. These microorganisms represent promising novel biobased solutions to replace environmentally harmful agrochemicals.

微生物是合成杀虫剂和肥料的一种环境可持续替代品。本研究的重点是从蛭石堆肥茶和一种有机无土栽培甜瓜作物的根瘤菌层中鉴定和筛选出具有在农业中用作生物农药和生物肥料潜力的微生物。评估了这些微生物的生物肥料能力，如固氮、磷酸盐溶解和苷元生产。作为生物农药评估的一部分，还测试了这些微生物对八种细菌和真菌植物病原体的体外拮抗作用。此外，还对所选菌株产生的具有农艺学意义的次生代谢物进行了预测性研究。细菌 Acinetobacter beijerinckii B9、Kocuria palustris B23、Pantoea agglomerans B32、Rahnella aquatilis B21、Streptomyces griseorubiginosus B29 和 Bacillus licheniformis B15，以及真菌 Aspergillus fumigatus H4、Aspergillus niger H6 和 Aspergillus terreus H2 都表现出卓越的生物杀虫和生物肥料活性。其中，所选真菌和丝状菌 S. griseorubiginosus B29 尤为突出，它们产生了许多与抗菌活性和诱导植物防御机制有关的次级代谢产物。这些微生物是替代对环境有害的农用化学品的新型生物解决方案，前景广阔。

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引用次数: 0