Biocatalysis and agricultural biotechnology最新文献_第7页

Biodegradation of ibuprofen from Trichoderma pseudoasperelloides CBMAI 2752 and Penicillium sp. cf. guaibinense CBMAI 2758 isolated from amazonian soil 亚马逊土壤中假曲霉CBMAI 2752和绿皮青霉CBMAI 2758对布洛芬的生物降解研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103871

Beatriz L. Ferreira , Adriana M. Ferreira , Fabricio H. Holanda , Patrícia de A. Nóbrega , Thiago A.M. Brito , Josean F. Tavares , Willian G. Birolli , André Luiz M. Porto , Lilian Grace da S. Solon , Irlon M. Ferreira

Ibuprofen (IBU) is one of the most widely consumed non-steroidal anti-inflammatory drugs (NSAIDs) worldwide, and its improper disposal has raised significant concern for the World Health Organization. In recent years, several technologies have been developed as alternatives for managing pharmaceutical-derived organic micropollutants (OMPs). This study investigates the biodegradation and biotransformation of IBU mediated by whole-cells of fungi isolated from iron mine soils in the Amazon region. The strains Trichoderma pseudoasperelloides CBMAI 2752 and Penicillium sp. cf. guaibinense CBMAI 2758 were selected for biodegradation experiments using a three-variable Box-Behnken factorial design. Both strains achieved over 99 % IBU biodegradation under multiple conditions, with pH emerging as a key parameter influencing efficiency. The positive Pareto chart values (6.74 and 4.65) indicated that lower pH values enhanced the biodegradation process. Three potential metabolites were identified by LC–MS, including 2-(2-hydroxy-4-isobutylphenyl)propanoic acid. Toxicity assays with zebrafish embryos revealed that extracts obtained after IBU biodegradation and biotransformation were non-toxic at the tested concentrations, allowing normal embryonic development. In contrast, commercial IBU was classified as toxic, with no embryo formation observed. Overall, this research highlights the remarkable biotechnological potential of Amazonian soil fungi for the efficient degradation of IBU, offering an ecologically sustainable solution to mitigate pharmaceutical contamination in global aquatic environments.

布洛芬（IBU）是世界上使用最广泛的非甾体抗炎药（NSAIDs）之一，其处置不当引起了世界卫生组织的严重关注。近年来，已经开发了几种技术作为管理药物来源的有机微污染物（OMPs）的替代方案。本研究研究了从亚马逊地区铁矿土壤中分离的真菌全细胞介导的IBU的生物降解和转化。采用三变量Box-Behnken析因设计，选取假曲霉（Trichoderma pseudoasperelloides） CBMAI 2752和愈创木青霉（Penicillium sp. cf. guaibinense） CBMAI 2758进行生物降解试验。两株菌株在多种条件下均实现了99%以上的IBU生物降解，其中pH值成为影响效率的关键参数。帕累托图阳性值（6.74和4.65）表明，较低的pH值促进了生物降解过程。通过LC-MS鉴定出3种潜在代谢物，包括2-（2-羟基-4-异丁基苯基）丙酸。对斑马鱼胚胎的毒性试验表明，经过IBU生物降解和生物转化后获得的提取物在测试浓度下是无毒的，允许正常的胚胎发育。相比之下，商业IBU被归类为有毒，未观察到胚胎形成。总体而言，本研究突出了亚马逊土壤真菌在有效降解IBU方面的显著生物技术潜力，为减轻全球水生环境中的药物污染提供了生态可持续的解决方案。

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引用次数: 0

Immobilization and characteristics of Trichoderma longibrachiatum pectinase on periwinkle (Tympanotonos fuscatus) shell-derived chitosan support 长尾木霉果胶酶在长春花壳源壳聚糖载体上的固定化及特性研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103870

Chukwunonso Anthony Nsude , Arinze Linus Ezugwu , Samson Chinekwu Onoyima , Ozoemena Emmanuel Eje , Innocent Izuchukwu Ujah , Cosmas Ezekaibeya Achikanu , Moses Obinna Ezenwali , Lotanna Chidera Okwesili , Sabinus Oscar Onyebuchi Eze , Ikechukwu Noel Emmanuel Onwurah , Ferdinand Chiemeka Chilaka

Industrial applications of soluble pectinases face drawbacks due to losses in catalytic activity, downstream processing of products, and enzymes' non-recoverability. Immobilizing pectinases on chitosan support can circumvent these limitations and enhance the stability, recoverability, and reusability of the enzymes. This study focuses on immobilization of pectinase from Trichoderma longibrachiatum onto chitosan support. Crude pectinase extracted from submerged fermentation of mango pectin by T. longibrachiatum was partially purified via Sephadex G-200 gel filtration chromatography. Chitosan extracted by deacetylation of chitin from periwinkle (Tympanotonos fuscatus NCBI: txid496830) shells gave 66.79 ± 0.70 % yield, 76.00 ± 0.87 % solubility in 1 % acetic acid solution, and a degree of deacetylation (DDA) of 61.67 %. FTIR spectroscopic analysis revealed an absorption maximum at 1636.3 cm⁻¹, corresponding to the NH₂ bending of primary amine in chitosan. Partially purified pectinase was immobilized by adsorption on chitosan beads. Chitosan beads prepared in NaOH-methanol solution gave better immobilized pectinase activity (48.22 μmol/min) than those prepared in NaOH-ethanol solution (16.82 μmol/min) and were then adopted for bead formations. Maximal enzyme immobilization was achieved at contact pH (5.0) and time (3 h) with pectinase-specific activities of 0.55 ± 0.01 U/mg and 1.44 ± 0.06 U/mg, respectively. An immobilization efficiency (IE) of 80.12 % was achieved at optimal pH (5.0) and temperature (40^oC). The immobilized pectinase retained 57.19 ± 0.24 % of its original activity after three cycles of reuse. The results of this study revealed that the productions of pectinase and chitosan from locally sourced T. longibrachiatum and Tympanotonos fuscatus shells (respectively) are cost-effective and that the reusability of immobilized pectinase makes it robust for biotechnological applications.

可溶性果胶酶的工业应用面临着催化活性损失、产品下游加工和酶的不可恢复性等缺点。壳聚糖载体固定化果胶酶可以克服这些限制，提高酶的稳定性、可恢复性和可重复使用性。研究了壳聚糖载体固定化长尾木霉果胶酶的方法。采用Sephadex G-200凝胶过滤层析法对芒果果胶深层发酵中提取的粗果胶酶进行了部分纯化。以长春花（Tympanotonos fuscatus NCBI: txid496830）壳聚糖为原料，经去乙酰化提取壳聚糖的收率为66.79±0.70%，在1%醋酸溶液中的溶解度为76.00±0.87%，去乙酰化度（DDA）为61.67%。FTIR光谱分析表明，壳聚糖在1636.3 cm−1处的吸收最大值与壳聚糖中伯胺的NH2弯曲相对应。用壳聚糖吸附固定化部分纯化的果胶酶。甲醇溶液制备的壳聚糖微球比乙醇溶液（16.82 μmol/min）具有更好的固定化果胶酶活性（48.22 μmol/min），可用于壳聚糖微球的形成。在接触pH（5.0）和接触时间（3 h）下，固定化效果最佳，果胶酶特异性活性分别为0.55±0.01 U/mg和1.44±0.06 U/mg。在最佳pH（5.0）和温度（40℃）条件下，固定化效率（IE）为80.12%。经3次循环使用后，固定化果胶酶的活性保持了57.19±0.24%。本研究结果表明，利用当地的长尾竹和褐斑鼓壳分别生产果胶酶和壳聚糖具有成本效益，并且固定化果胶酶的可重复使用性使其具有良好的生物技术应用前景。

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引用次数: 0

Coffee fermentation using polyethylene bags as a bioreactor: effect of adding coffee peels and a starter culture 用聚乙烯袋作为生物反应器的咖啡发酵：添加咖啡皮和发酵剂的效果

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103869

Antônio Sergio Souza , José Maria Rodrigues da Luz , Fabrícia Queiroz Mendes , Willian dos Santos Gomes , Lucas Louzada Pereira , Paulo Sérgio Monteiro , Evandro Galvão Tavares Menezes , André Mundstock Xavier de Carvalho

Due to the popularization of specialty coffees, producers began to invest in the development of post-harvest processing techniques to improve the chemical and sensory characteristics of the coffee beverage. Thus, the objective of this study was to evaluate the effect of using coffee peels and a starter culture of Saccharomyces cerevisiae as inocula in coffee fermentation, conducted in polyethylene bags as a bioreactor, on the microbiological, physical, chemical, and sensory parameters of the coffee. The use of coffee peels as an inoculum source and the choice of this yeast were based on our previous studies. Peeled coffee cherries without or with the addition of peels were used in spontaneous and induced fermentations by S. cerevisiae at 30 °C Fermentation times were 24, 48, 72, 96, and 120 h. A significant yeast colony count after 120 h of fermentation was observed only in the musts from S. cerevisiae-induced fermentations. The addition of the coffee bark also did not influence the viable microbial cell count. A reduction in pH, Brix, and average temperature values was observed in the wort during fermentation. The bands in the infrared spectra in the regions of 1317 and 1095 cm⁻¹ and 1093 and 873 cm⁻¹ showed the chemical differences between the treatments after 120 h of fermentation. S. cerevisiae-induced fermentations had a greater contribution to the sensory perceptions of the coffee beverage than spontaneous fermentation. Thus, fermentation efficiency was directly proportional to fermentation time, anaerobic environment, and inoculum source (endogenous coffee cherry or starter culture).

由于精品咖啡的普及，生产商开始投资开发采收后的加工技术，以提高咖啡饮料的化学和感官特性。因此，本研究的目的是评估使用咖啡皮和酿酒酵母发酵剂作为接种剂，在聚乙烯袋中作为生物反应器进行咖啡发酵，对咖啡的微生物，物理，化学和感官参数的影响。使用咖啡皮作为接种源和酵母的选择是基于我们之前的研究。在30℃条件下，将去皮的咖啡樱桃与不加果皮的咖啡樱桃分别进行自发发酵和诱导发酵，发酵时间分别为24、48、72、96和120 h。发酵120 h后，仅在酿酒酵母诱导发酵的酵母菌中观察到显著的酵母菌落计数。咖啡皮的添加也不影响活菌细胞数。在发酵过程中观察到麦芽汁的pH值、糖度和平均温度值的降低。发酵120 h后，1317和1095 cm−1以及1093和873 cm−1区域的红外光谱显示了不同处理之间的化学差异。酿酒酵母诱导的发酵比自发发酵对咖啡饮料的感官知觉有更大的贡献。因此，发酵效率与发酵时间、厌氧环境和接种物来源（内源性咖啡樱桃或发酵剂）成正比。

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引用次数: 0

Dose-dependent effects of Bacillus subtilis SB008 on root development, photosynthetic efficiency, and antioxidant activity in soybean plants 枯草芽孢杆菌SB008对大豆根系发育、光合效率和抗氧化活性的剂量依赖性影响

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103878

João Arthur dos Santos Oliveira , Erika Fritegotto Leite , Giovana Gomes Ferreira Matos , Natieli Jenifer Mateus Corniani , Carolina Fedrigo Coneglian , Maria Eduarda de Souza Rocha , Patricia Viero , Luana Thais Varize Marcusso , Carlos Augusto Corniani da Silva , Willian Martire Marcusso

This study demonstrates the biotechnological potential of the endophytic bacterium Bacillus subtilis SB008 as a multifunctional bioinoculant for sustainable agriculture. The strain produced key metabolites, including indole-3-acetic acid (3.3 μg/mL), exopolysaccharides (361 μg/mL), siderophores (75.7 %), and amino acids, alongside hydrolytic enzymes (amylase, cellulase, protease, and chitinase). Polynomial regression modeling revealed dose-dependent effects: seed inoculation (1 mL/kg) enhanced root system development, while single foliar application (0.220 L/ha) enhanced chlorophyll accumulation. Notably, foliar treatment at 0.160 L/ha elicited a robust antioxidant response, simultaneously increasing catalase (1354 nmol/min/μg protein) and superoxide dismutase (3075 nmol/min/μg protein) activities while maintaining balanced peroxidase levels (7715 nmol/min/μg protein). These findings suggest that SB008 functions as a dual-purpose bioinoculant, enhancing plant development through morphological effects on root architecture and biochemical modulation of photosynthesis and antioxidant enzymes. Its compatibility with soybean cultivation suggests promise for integration into sustainable management systems.

该研究证明了内生细菌枯草芽孢杆菌SB008作为可持续农业多功能生物接种剂的生物技术潜力。该菌株主要代谢产物包括吲哚-3-乙酸（3.3 μg/mL）、胞外多糖（361 μg/mL）、铁载体（75.7%）和氨基酸，以及水解酶（淀粉酶、纤维素酶、蛋白酶和几丁质酶）。多项式回归模型显示剂量依赖性效应：种子接种（1 mL/kg）促进根系发育，单叶接种（0.220 L/ha）促进叶绿素积累。值得注意的是，叶面浓度为0.160 L/ha的处理引起了强烈的抗氧化反应，同时增加了过氧化氢酶（1354 nmol/min/μg蛋白）和超氧化物歧化酶（3075 nmol/min/μg蛋白）活性，同时保持了平衡的过氧化物酶水平（7715 nmol/min/μg蛋白）。上述结果表明，SB008作为一种具有双重作用的生物接种剂，通过对根系形态的影响以及光合作用和抗氧化酶的生化调节来促进植物发育。它与大豆种植的兼容性表明，它有望纳入可持续管理系统。

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引用次数: 0

Sargassum wightii Greville promotes tomato root growth by alleviating urea-induced soil acidification in tropical red soil 马尾藻通过缓解尿素引起的热带红壤土壤酸化促进番茄根系生长

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103867

Kanagaraj Muthu-Pandian Chanthini , Ponnusamy Murugan , Ganesh-Subbaraja Pavithra , Arulsoosairaj Deva-Andrews , Narayanan Thulasi-Raman , Sengottayan Senthil-Nathan

Soil health is fundamental to agricultural productivity, yet intensive fertilisation practices accelerate degradation, particularly through urea-driven acidification, which lowers pH, reduces nutrient availability, and increases metal ion toxicities. This study evaluated the potential of Sargassum wightii Greville extract (SgWE) to remediate acidified tropical red soils and enhance tomato growth. Soils from a monocropped tomato field (pH 4.8) were amended with SgWE (80 %) and compared against untreated acid soil, a control, and a commercial seaweed biostimulant. Application of SgWE significantly increased soil pH and nutrient availability, while mitigating the adverse effects of H⁺ ion accumulation. Tomato seedlings grown in SgWE-treated soils showed higher germination rates, greater root biomass and architecture, enhanced shoot growth, and elevated chlorophyll content. Qualitative analyses, including SEM–EDS imaging, indicated improved root membrane integrity and reduced Al deposition, while microbial plate counts revealed increased microbial populations under SgWE treatment. Physiological assays demonstrated increased activities of antioxidant enzymes (superoxide dismutase, peroxidase) and reduced lipid peroxidation (MDA), suggesting improved stress tolerance. Together, these findings indicate that SgWE functions as an ecofriendly amendment that restores acidic soils and supports crop productivity.

土壤健康是农业生产力的基础，但密集施肥做法加速了土壤退化，特别是通过尿素驱动的酸化，这会降低pH值，减少养分供应，并增加金属离子毒性。本研究评价了马尾藻提取物（SgWE）修复酸化热带红壤和促进番茄生长的潜力。在一个单作番茄田（pH值4.8）的土壤中添加80%的SgWE，并与未经处理的酸性土壤、对照土壤和商业海藻生物刺激素进行比较。施用SgWE显著提高了土壤pH值和养分有效性，同时减轻了H+离子积累的不利影响。在sgwe处理的土壤中生长的番茄幼苗具有更高的发芽率、更大的根生物量和结构、更强的芽生长和更高的叶绿素含量。定性分析，包括SEM-EDS成像，表明根膜完整性改善，Al沉积减少，而微生物板计数显示SgWE处理下微生物种群增加。生理实验表明，抗氧化酶（超氧化物歧化酶、过氧化物酶）活性增加，脂质过氧化（MDA）活性降低，表明胁迫耐受性提高。总之，这些发现表明，SgWE是一种生态友好型改良剂，可以恢复酸性土壤并支持作物生产力。

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引用次数: 0

Production metrics of lemongrass essential oil under the combined influence of organics and silicon nanoparticles 有机和纳米硅复合作用下柠檬草精油的生产指标

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103835

Soumik Samanta , Kaushik Maity , Sangam Subba Lawti , Sujit Kumar Bhunia , Manish Kumar Naskar , Nilanjan Biswas , Apurba Bandyopadhyay , Amit Baran Sharangi

Lemongrass, containing citral and geraniol, offers huge medicinal benefits, making it popular in food preservation, cosmetics, and pharmaceuticals. Lemongrass in Eastern India faces challenges due to nutrient deficiencies and environmental fluctuations. To address these challenges and enhance productivity, sustainable strategies such as the use of silicon nanoparticles (SiNPs) and organic inputs like vermicompost (VC) and farmyard manure(FYM) are being investigated. These approaches enhance agronomic traits, essential oil and nutrient absorption by stimulating secondary metabolites in aromatic plants suitable for these agro-climatic conditions. This study addresses a critical gap by investigating the combinations of SiNPs and organic inputs that enhance nutrient bioavailability and interact with seasonal weather patterns. Using a randomized complete block design with eleven treatments and three replications, the findings indicated that combining FYM (30 t ha⁻¹) with SiNPs (200 mg L⁻¹) significantly plant height (13.04 %), tillers plant⁻¹(48.64 %), leaves plant⁻¹(51.28 %), leaf area index (96.40 %), biomass (61.29 %), and essential oil (33.33 %) compared to control. The interaction between VC (30 t ha⁻¹) and SiNPs(200 mg L⁻¹) notably enhanced plant height (14.70 %), tillers plant⁻¹(55.01 %), leaves plant⁻¹(55.28 %), and leaf area index (135.30 %), suggesting better light interception and nutrient-driven biomass in response to the control. VC and SiNPs produced 82.79 % more biomass and 116.67 % more oil than the control, underscoring the effectiveness of these interventions in sustaining productivity. Favourable weather conditions, including moderate rainfall and temperatures, enhanced these benefits. By integrating VC with SiNPs, this approach optimizes nutrient use and economic returns, offering an eco-friendly solution for improving lemongrass cultivation in resource-limited agroecosystems.

柠檬草含有柠檬醛和香叶醇，具有巨大的药用价值，因此在食品保鲜、化妆品和制药方面很受欢迎。由于营养缺乏和环境波动，印度东部的柠檬草面临挑战。为了应对这些挑战并提高生产力，正在研究诸如使用硅纳米颗粒（SiNPs）和有机投入物（如蚯蚓堆肥（VC）和农家肥（FYM））等可持续战略。这些方法通过刺激适合这些农业气候条件的芳香植物的次生代谢物来提高农艺性状、精油和营养吸收。本研究通过调查SiNPs和有机输入的组合来提高养分生物利用度并与季节性天气模式相互作用，从而解决了一个关键的空白。采用11个处理、3个重复的随机完全区组设计，结果表明：与对照相比，FYM （30 tha−1）与SiNPs （200 mg L−1）组合显著提高了株高（13.04%）、分蘖株−1（48.64%）、叶片株−1（51.28%）、叶面积指数（96.40%）、生物量（61.29%）和挥发油（33.33%）。VC （30 t ha−1）和SiNPs（200 mg L−1）的交互作用显著提高了植株高度（14.70%）、分蘖株- 1（55.01%）、叶片株- 1（55.28%）和叶面积指数（135.30%），表明对照具有更好的光截留和养分驱动生物量。与对照相比，VC和SiNPs的生物量增加了82.79%，产油量增加了116.67%，这表明这些干预措施在维持生产力方面是有效的。有利的天气条件，包括适度的降雨和温度，增强了这些好处。通过将VC与sinp相结合，该方法优化了养分利用和经济回报，为在资源有限的农业生态系统中改善柠檬草种植提供了一种生态友好的解决方案。

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引用次数: 0

Somatic hybridization: a Cinderella of plant biotechnology? 体细胞杂交：植物生物技术的灰姑娘？

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103875

Elena Rakosy-Tican, Daniel Cruceriu, Imola Molnar

Somatic hybridization is a plant biotechnological technique that belongs to genetic manipulation/improvement since it involves the genetic manipulation of naked plant cells or protoplasts. Protoplast from two different species or genotypes can be fused and somatic hybrid plants regenerated based on plant somatic cell totipotency. The first somatic hybrids were obtained and regenerated in the 1970s and many groups of crops and horticultural species have benefited from the isolation of protoplasts, their fusion and regeneration of improved somatic or parasexual hybrids. In some crops, such as potato, this genetic manipulation tool has produced good results with exploitation of wild Solanum species as donors of resistance genes. Still, there are many reviews on plant biotechnology that only discuss the most recent techniques, such as plant genetic transformation, RNAi and genome editing techniques, as successful plant biotechnologies. In this review we aim to discuss some of the reasons for the neglect shown to somatic hybridization, a technique able to bypass pre- and postzygotic sexual incompatibility barriers between related but incongruent species and to facilitate the introgression of multiple genes (i.e. QTLs) or multiple resistance traits into the gene pool of different crops. We also discuss the new role of this technique in the genomic era, and how this Cinderella of plant biotechnology can benefit plant breeding as a non-GMO technology in a complex, combinatorial biotechnological scheme under the urgent challenges of climate change and population growth.

体细胞杂交是一种植物生物技术，它涉及对植物裸细胞或原生质体进行遗传操作，属于遗传操作/改良。利用植物体细胞的全能性，可以实现两种不同物种或基因型原生质体的融合和体细胞杂交植株的再生。第一批体细胞杂交种是在20世纪70年代获得和再生的，许多作物和园艺物种都受益于原生质体的分离、它们的融合和体细胞或拟性杂交种的再生。在某些作物中，如马铃薯，这种遗传操作工具在利用野生茄属植物作为抗性基因供体方面取得了良好的效果。然而，关于植物生物技术的许多评论只讨论了最新的技术，如植物遗传转化、RNAi和基因组编辑技术，作为成功的植物生物技术。在这篇综述中，我们的目的是讨论一些忽视体细胞杂交的原因，体细胞杂交是一种技术，能够绕过亲缘但不一致的物种之间的合子前和合子后性不亲和障碍，并促进多个基因（即qtl）或多个抗性性状渗入不同作物的基因库。我们还讨论了这种技术在基因组时代的新作用，以及在气候变化和人口增长的紧迫挑战下，这种植物生物技术的灰姑娘如何在复杂的、组合的生物技术方案中有益于植物育种。

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引用次数: 0

Application of feruloyl esterase BpFae03105 and xylanase XynAS Co-enzymolysis to produce ferulic acid from wheat bran 应用阿魏酰酯酶BpFae03105和木聚糖酶XynAS辅酶解麦麸制备阿魏酸

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103868

Han Lichun , Zhong Xiufang , Fu Zhilei , Yan Jinlong , Xiao Lin , Zhu Yuting , Liu Xiaoyan , Rana Abdul Basit , Wang Jinpeng , Yin Dafeng , Fan Guangsen

Wheat bran, rich in phenolic compounds like ferulic acid (FA), faces limited bioconversion due to its recalcitrant lignocellulosic structure. This study developed a synergistic catalytic system using feruloyl esterase (BpFae03105) and xylanase (XynAS) for efficient conversion into FA and xylo-oligosaccharides (XOS). A multi-stage optimization strategy was employed. First, the effective ranges of eight basic parameters were determined through single-factor experiments. Then, Plackett-Burman design was used to screen for significant factors influencing FA yield, identifying enzymatic hydrolysis temperature, time, and enzyme addition ratio as the most critical. The conditions were further optimized using the Box-Behnken response surface method, resulting in the following optimal conditions: enzymatic hydrolysis temperature of 43 °C, reaction time of 4.2 h, shaker rotational speed of 120 rpm, pH 5.0 phosphate buffer, solid-to-liquid ratio of 1:115 (w/v), enzyme addition ratio (BpFae03105:XynAS) of 1:11.2, BpFae03105 concentration of 8 U/mL, and wheat bran particle size greater than 20 mesh. Under these conditions, the FA yield reached 77.98 %, a 2.65-fold increase compared to the unoptimized process. In addition, XOS production achieved a substantial yield of 26.24 %, marking a significant breakthrough in co-product generation. The proposed mechanism suggests XynAS degrades xylan, disrupting the lignocellulosic matrix and exposing feruloyl ester groups, thereby enhancing substrate accessibility and BpFae03105 catalytic efficiency. The synergistic enzymatic action overcame limitations of traditional individual enzymes systems, particularly product inhibition, enabling integrated wheat bran valorization. Overall, this study provides a novel, scalable biorefinery approach for high-value utilization of agricultural by-products, offering economic and environmental benefits for sustainable biomass processing.

麦麸富含阿魏酸（FA）等酚类化合物，由于其顽固的木质纤维素结构，其生物转化受到限制。本研究建立了阿铁酰酯酶（BpFae03105）和木聚糖酶（XynAS）协同催化体系，高效转化FA和低聚木糖（XOS）。采用多阶段优化策略。首先，通过单因素实验确定8个基本参数的有效范围；然后采用Plackett-Burman设计筛选影响FA产率的显著因素，确定酶解温度、酶解时间和酶加比为最关键因素。采用Box-Behnken响应面法进一步优化条件，得到酶解温度43℃，反应时间4.2 h，振动筛转速120 rpm，磷酸盐缓冲液pH 5.0，料液比1:15 15 (w/v)，酶加比（BpFae03105:XynAS） 1:11.2， BpFae03105浓度8 U/mL，麦麸粒径大于20 mesh。在此条件下，FA收率达到77.98%，比未优化工艺提高了2.65倍。此外，XOS产量达到了26.24%，标志着副产物生成的重大突破。该机制表明，XynAS降解木聚糖，破坏木质纤维素基质并暴露阿铁酰酯基团，从而提高底物可及性和BpFae03105的催化效率。协同酶的作用克服了传统的单个酶系统的局限性，特别是产物抑制，使整体麦麸增值。总的来说，这项研究为农业副产品的高价值利用提供了一种新颖的、可扩展的生物炼制方法，为可持续的生物质加工提供了经济和环境效益。

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引用次数: 0

Phytotoxicity of Hesperozygis ringens (Benth.) Epling essential oil on Lolium multiflorum Lam 黄皮参的植物毒性研究（下）何首乌精油的研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103881

Maiqueli Longaretti Bonetz , Fernanda Bruxel , Mara Cíntia Winhelmann , Camila Thaís Scheibler , Shirley Martins Silva , Gabriela Larissa Kock , Luana Lermen Becchi , Julia Gastmann , Amanda Pichani Primaz , Lucélia Hoehne , Elisete Maria de Freitas

The development of alternative practices for weed management, such as the use of essential oil EO based herbicides, seeks to reduce dependence on synthetic herbicides and promote agricultural sustainability. In this context, we investigated the effects of Hesperozygis ringens essential oil on Lolium multiflorum. The essential oil was obtained by hydrodistillation, and then subjected to bioassays of germination and initial seedling development exposed to the essential oil at the indicated concentrations of 0.0 %, 0.001 %, 0.0025 %, 0.005 %, 0.01 %, and 0.02 %; as well as in vitro tests with adult plants (0.0 %, 0.02 %, and 0.04 %); and field tests (1.0 % EO, conventional herbicide ammonium glufosinate and water, water and an adjuvant, and only water). Chemical characterization of the EO identified 29 compounds, the major was pulegone (70.65 %), limonene (4.49 %), bicyclogermacrene (2.54 %), linalool (2.45 %), and (E)-caryophyllene (2.27 %). The EO had a phytotoxic effect on L. multiflorum starting at 0.001 %, with higher efficacy at the concentrations of 0.01 % and 0.02 %. At these concentrations, there was complete inhibition of germination and seedling formation, significant reduction in growth of roots and aerial parts of seedlings, decreased chlorophyll and dry mass values, as well as damages to cell membranes. In the field tests, there was decreased chlorophyll and relevant anatomical changes in plant tissue; cell lesions observed in anatomical cuts were similar to those caused by synthetic herbicides, and similar to those observed in the treatment with glufosinate. H. ringens EO was shown to be promising for the development of a natural herbicide.

开发杂草管理的替代方法，例如使用精油基除草剂，旨在减少对合成除草剂的依赖，促进农业的可持续性。以何首乌为研究对象，研究了何首乌精油对何首乌生长的影响。通过加氢蒸馏法获得精油，并在精油浓度为0.0%、0.001 %、0.0025%、0.005%、0.01%和0.02%的条件下进行萌发和幼苗发育的生物测定；以及成年植物的体外试验（0.0%，0.02%和0.04%）；现场试验（1.0% EO，常规除草剂草甘膦铵和水，水和助剂，和只有水）。化学性质鉴定出29个化合物，主要为普莱酮（70.65%）、柠檬烯（4.49%）、双环绿烯（2.54%）、芳樟醇（2.45%）和(E)-石竹烯（2.27%）。从0.001%浓度开始，EO对何首乌具有一定的植物毒性，以0.01%和0.02%浓度的效果更好。在这些浓度下，萌发和成苗完全受到抑制，幼苗根系和地上部分的生长显著减少，叶绿素和干质量值下降，细胞膜受损。在田间试验中，叶绿素含量降低，植物组织发生了相关的解剖变化；解剖切口中观察到的细胞病变与合成除草剂引起的细胞病变相似，与草铵膦治疗中观察到的细胞病变相似。结果表明，绿根草具有开发天然除草剂的良好前景。

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引用次数: 0

Valorization of banana leaf (Musa spp. var. Cavendish): A comprehensive study of bioactive compounds and biological activities 香蕉叶（Musa spp. var. Cavendish）的增值：生物活性化合物和生物活性的综合研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 DOI: 10.1016/j.bcab.2025.103877

Thi-Dung Ha , Thi Hue Cao , Van Nhan Le , Y Doan Trang Tran , Hien Y Hoang

Banana leaf is an abundant, commonly discarded agricultural by-product with culinary and medicinal potential, yet its phytochemical composition and biological potential remain underexplored. This study aims to investigate the impact of drying temperature on the bioactive compounds and biological activity in banana leaf. Leaves were oven-dried at different temperatures or sun-dried, and polyphenol, flavonoid, and tannin content were quantified. Obtained results revealed that drying temperature significantly influenced the content of these compounds, with 50 °C identified as the optimal condition for preserving these bioactive compounds. GC-MS analysis of ethanol extract from leaf dried at 50 °C showed a high level of fatty acids (46.40 %), terpenes and derivatives (22.99 %), along with other bioactive constituents. Pathway enrichment analysis of detected compounds suggested potential biological functions of banana leaf. Extract of leaf dried at 50 °C showed the highest antioxidant activity (DPPH EC₅₀: 33.69–54.40 μg/mL; reducing power EC₅₀: 76.04–97.75 μg/mL, beta-carotene bleaching inhibition IC₅₀: 295.33 ± 7.35 μg/mL), NO-production inhibition (IC₅₀: 43.79–68.09 μg/mL) with no detectable cytotoxicity in vitro, and antifungal activity against Candida albicans (MIC: 256 μg/mL). These findings highlight that banana leaf possesses potent biological potential, and oven-drying at 50 °C is the most effective drying temperature for retaining banana leaf's bioactive properties, supporting its potential application in food and pharmacy while promoting sustainable agricultural waste utilization.

香蕉叶是一种丰富的、通常被丢弃的农业副产品，具有烹饪和药用潜力，但其植物化学成分和生物潜力仍未得到充分开发。本研究旨在探讨干燥温度对香蕉叶中生物活性成分及生物活性的影响。将叶片在不同温度下烘干或晒干，定量测定多酚、类黄酮和单宁含量。结果表明，干燥温度对这些化合物的含量有显著影响，50℃是保存这些生物活性化合物的最佳条件。50°C干燥的乙醇提取物的GC-MS分析显示，脂肪酸（46.40%），萜烯及其衍生物（22.99%）含量高，以及其他生物活性成分。途径富集分析表明，香蕉叶具有潜在的生物学功能。50℃干燥叶提取物抗氧化活性最高（DPPH EC50: 33.69 ~ 54.40 μg/mL，还原力EC50: 76.04 ~ 97.75 μg/mL）， β -胡萝卜素漂白抑制作用IC50: 295.33±7.35 μg/mL)， no生成抑制作用（IC50: 43.79 ~ 68.09 μg/mL），体外无细胞毒性，抗白色念珠菌活性最高（MIC: 256 μg/mL）。这些研究结果表明，香蕉叶具有强大的生物潜力，50°C的干燥温度是保持香蕉叶生物活性特性的最有效干燥温度，支持其在食品和制药方面的潜在应用，同时促进农业废弃物的可持续利用。

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引用次数: 0