Biocatalysis and agricultural biotechnology最新文献_第6页

Optimization of ultrasound pretreatment for enzymatic hydrolysis of Bambara groundnut protein isolate by hybrid catfish viscera trypsin and characterization of the hydrolysate 杂化鲶鱼内脏胰蛋白酶水解班巴拉花生分离蛋白的超声预处理优化及水解产物的表征

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-20 DOI: 10.1016/j.bcab.2025.103897

Sakonwat Kuepethkaew , Sappasith Klomklao , Soottawat Benjakul , Hideki Kishimura

The objective of this study was to investigate and optimize the effect of ultrasound (US) pretreatment on the enzymatic hydrolysis of Bambara groundnut (Vigna subterranea (L.) Verdc.) protein isolate (BGPI) using trypsin from hybrid catfish (Clarias macrocephalus × Clarias gariepinus) viscera (HCVT). The optimal enzymatic hydrolysis conditions using HCVT alone were first determined at 60 °C and pH 9.5, with enzyme concentration and reaction time significantly influencing hydrolysis efficiency, measured by the degree of hydrolysis (DH). The optimal hydrolysis condition was achieved using 10 % HCVT for 25 min (p < 0.05). Subsequently, the influences of US pretreatment parameters including ultrasonic frequency, sonification duration, and temperature were examined prior to enzymatic hydrolysis. US pretreatment significantly improved DH, with the highest DH (74.55 %) obtained at 24 kHz and 30 °C for 20 min, which was significantly higher than the non-pretreated control (p < 0.05). In a comparison of the hydrolytic activities of HCVT and porcine trypsin under the same optimized US conditions, HCVT produced a slightly lower DH (p < 0.05). The resulting Bambara groundnut protein hydrolysate (BGPH), obtained under optimized US and HCVT conditions, was also characterized. BGPH exhibited high protein content (84.44 %, dry weight), elevated essential amino acids, particularly leucine, lysine, and valine, and low fat content (0.68 %, dry weight). The hydrolysate displayed a reddish-brown color. These findings demonstrate that US pretreatment effectively enhances enzymatic hydrolysis of BGPI by HCVT, improving both hydrolysis efficiency and nutritional quality, and highlight its potential application in producing high-value functional protein ingredients for the food industry.

本研究的目的是研究超声波预处理对板栗（Vigna subterranea (L.)）酶解的影响，并对其进行优化。用胰蛋白酶从杂交鲶鱼（claras macrocephalus × Clarias gariepinus）内脏（HCVT）中分离出Verdc.)蛋白（BGPI）。首先确定单独使用HCVT进行酶解的最佳条件为60℃，pH 9.5，酶浓度和反应时间对酶解效率有显著影响，以水解度（DH）衡量。最佳水解条件为10% HCVT，水解时间为25 min （p < 0.05）。随后，考察了酶解前超声频率、超声时间和温度等预处理参数的影响。US预处理显著提高了DH，在24 kHz、30°C、20 min时DH最高（74.55%），显著高于未预处理的对照组（p < 0.05）。在相同的US优化条件下，HCVT和猪胰蛋白酶的水解活性比较，HCVT产生的DH略低（p < 0.05）。在优化的US和HCVT条件下得到的Bambara花生蛋白水解产物（BGPH）也进行了表征。BGPH具有高蛋白质含量（84.44%，干重），高必需氨基酸含量，特别是亮氨酸，赖氨酸和缬氨酸，低脂肪含量（0.68%，干重）。水解产物呈红褐色。研究结果表明，US预处理能有效地促进HCVT酶解BGPI，提高水解效率和营养品质，在食品工业生产高价值功能性蛋白原料方面具有潜在的应用前景。

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引用次数: 0

Comparative evaluation of antioxidant and antidiabetic activities of kombucha fermented with three ginger (Zingiber officinale) varieties 三种生姜发酵康普茶抗氧化和抗糖尿病活性的比较研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-19 DOI: 10.1016/j.bcab.2025.103888

Hani Mulyani , Nina Artanti , Ratna Yuniati , Silmy Nasyitha , Yasman Yasman

Kombucha is a traditional fermented tea known for its health-promoting properties, including antioxidant and antidiabetic activities. Ginger (Zingiber officinale) is widely used as a functional ingredient in kombucha due to its rich phytochemical content. This study aimed to comparatively evaluate the antioxidant and antidiabetic activities of kombucha prepared using three ginger varieties—elephant, emprit, and red ginger—during an 18-day fermentation period. Each ginger extract was brewed in sugared black tea (Camellia sinensis), inoculated with a symbiotic culture of bacteria and yeast (SCOBY), and fermented at room temperature. Samples were collected at 0, 6, 12, and 18 days to analyze pH, total acidity, and total sugar content. Antioxidant activity was assessed using DPPH assays, while α-glucosidase inhibition was measured to evaluate antidiabetic potential. Results showed a consistent decline in pH and sugar levels with a concurrent increase in total acidity, indicating progressive microbial activity. Among the variants, elephant ginger kombucha displayed the highest antioxidant capacity and α-glucosidase inhibition, particularly on day 12, suggesting this as the optimal fermentation time for functional efficacy. Differences in activity among the ginger types were attributed to their unique phytochemical profiles, influencing fermentation dynamics and metabolite production. In conclusion, ginger-based kombucha, particularly from gajah variety, emerges as a promising functional beverage with potential health benefits related to oxidative stress reduction, glycemic control. These findings support the potential development of ginger-based kombucha as a functional beverage for managing oxidative stress and postprandial hyperglycemia.

康普茶是一种传统的发酵茶，以其促进健康的特性而闻名，包括抗氧化和抗糖尿病的活性。生姜（Zingiber officinale）因其丰富的植物化学成分而被广泛用作康普茶的功能成分。本研究旨在比较评价3种生姜品种——大象姜、埃普里特姜和红姜在18 d发酵过程中制备的康普茶的抗氧化和抗糖尿病活性。每种生姜提取物在加糖红茶（Camellia sinensis）中冲泡，接种细菌和酵母的共生培养物（SCOBY），并在室温下发酵。在第0、6、12和18天采集样品，分析pH、总酸度和总糖含量。采用DPPH测定抗氧化活性，α-葡萄糖苷酶抑制测定抗糖尿病潜能。结果显示，pH值和糖水平持续下降，同时总酸度增加，表明微生物活动不断增加。其中，大象姜康普茶的抗氧化能力和α-葡萄糖苷酶抑制能力最高，特别是在第12天，这表明这是功能功效的最佳发酵时间。不同类型姜的活性差异归因于其独特的植物化学特征，影响发酵动力学和代谢物的产生。总之，生姜康普茶，特别是来自gajah品种的康普茶，是一种很有前途的功能性饮料，具有潜在的健康益处，可以减少氧化应激，控制血糖。这些发现支持了姜汁康普茶作为控制氧化应激和餐后高血糖的功能性饮料的发展潜力。

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引用次数: 0

Effect of thermal alkaline and thermal hydrolysis on the methane production and antibiotic resistance genes removal during sewage sludge anaerobic digestion 热碱性和热水解对污泥厌氧消化过程中甲烷生成和抗生素抗性基因去除的影响

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-18 DOI: 10.1016/j.bcab.2025.103891

Ruoxin Li , Chunsheng Qiu , Nannan Liu , Wen Zhang , Xu Chen , Yaping Zhang , Chenchen Wang , Shaopo Wang

Anaerobic digestion technology has gained wide attention as a potential method to reduce the spread of antibiotic resistance genes (ARGs). Thermal alkaline hydrolysis and thermal hydrolysis pretreatments were selected for pretreatment of sewage sludge, the effects of different pretreatment methods on anaerobic digestion efficiency and ARGs removal in the combined pretreatment-anaerobic digestion process of sludge was investigated in this study. Metagenomic sequencing and quantitative PCR were used to systematically analyze the dissemination of ARGs in different sludge samples. The results showed that both thermal alkaline hydrolysis and thermal hydrolysis pretreatments could promote the efficiency of sludge anaerobic digestion, with the cumulative methane production increased by 44.20 % and 58.73 %, respectively, compared with the raw sludge. Moreover, the absolute abundance of ARGs was reduced by 19.52 % and 98.97 % by thermal alkaline hydrolysis and thermal hydrolysis pretreatments, respectively, compared with the raw sludge. Although a rebound of the reduced ARGs occurred in the subsequent anaerobic digestion process, compared with the raw sludge, the removal efficiency of the absolute abundance of ARGs by thermal hydrolysis-anaerobic digestion (91.98 %) was still higher than that of the thermal alkaline hydrolysis-anaerobic digestion process (89.62 %). The results of the correlation analysis indicated a significant negative correlation between pH value and tetG as well as tetX (p < 0.01). Additionally, horizontal gene transfer mediated by intI1 was found to significantly promote the dissemination of ARGs. It also revealed that there was a significant correlation between the fate of ARGs and the succession of microbial community structure.

厌氧消化技术作为一种潜在的减少抗生素耐药基因（ARGs）传播的方法受到了广泛的关注。选择热碱性水解和热水解预处理对污泥进行预处理，研究不同预处理方法对污泥预处理-厌氧消化联合工艺中厌氧消化效率和ARGs去除率的影响。采用宏基因组测序和定量PCR技术对不同污泥样品中ARGs的分布进行了系统分析。结果表明，热碱性水解和热水解预处理均能提高污泥厌氧消化效率，累计产甲烷量较原污泥分别提高44.20%和58.73%。与原液相比，热碱性水解和热水解预处理分别使ARGs的绝对丰度降低了19.52%和98.97%。虽然在随后的厌氧消化过程中还原后的ARGs出现反弹，但与原污泥相比，热水解-厌氧消化对ARGs绝对丰度的去除效率（91.98%）仍高于热碱性水解-厌氧消化工艺（89.62%）。相关性分析结果表明，pH值与tetG、tetX呈极显著负相关（p < 0.01）。此外，发现由intI1介导的水平基因转移可显著促进ARGs的传播。这也揭示了ARGs的命运与微生物群落结构的演替有显著的相关性。

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引用次数: 0

Heavy metal tolerance, alkane biodegradation, and biosurfactant production by Chryseomicrobium amylolyticum RP2 isolated from industrial effluents 从工业废水中分离的溶淀粉黄菌RP2的重金属耐受性、烷烃生物降解和生物表面活性剂生产

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-17 DOI: 10.1016/j.bcab.2025.103892

Ranjan Pandey , Bhaskar Krishnamurthy , Abdullah A. Alarfaj , Mohammad Javed Ansari , Daizy Rani Batish , Harminder Pal Singh

The current study examined the heavy metal tolerance, biodegradation potential, and biosurfactant production of a strain of bacteria isolated from industrial effluents. The 16S rRNA analysis identified the strain as Chryseomicrobium amylolyticum RP2. The strain RP2 exhibited a tolerance of at least 0.5 g/L towards various elements, including As⁵⁺, Fe²⁺, Mo⁶⁺, Pb²⁺, Co²⁺, Cu²⁺, Mn²⁺, Ni²⁺, and Zn²⁺. The isolate emulsified several organic substrates, such as aviation turbine fuel, diesel, and xylene, with an emulsification index above 60%. The fluorescence microscopy showed that the RP2 strain degrades ≥95% of the model hydrocarbons (n-hexadecane, diesel, and kerosene) within 14 days of incubation. The biosurfactant was rich in hexa-, octadecenoic, and octadecadienoic acids, and was stable at pH 6–10 and 20–60 °C temperature. NMR, FTIR, and MALDI-TOF analyzes showed that the biosurfactant was mostly lipid and peptide but also contained minor carbohydrate residues, making the compound more accurately described as lipopeptide-like, complex lipo-glyco-lipid-like, or mixed-type biosurfactants. At a critical micelle concentration of 0.380 mg/mL, the biosurfactant effectively reduced the surface tension of water to 29.56 mN/m. This study is the first to report the biosurfactant properties and remediation potential of a heavy metal-tolerant RP2 strain of C. amylolyticum. The results of the present study reflect as preliminary evidence that the C. amylolyticum RP2 strain, with its resistance to heavy metals and effective remediation capability, could remove organic pollutants; however, the presence of genes responsible for the degradation of alkanes and further investigation into the industrial applications of biosurfactant is necessary.

本研究考察了从工业废水中分离出的一株细菌的重金属耐受性、生物降解潜力和生物表面活性剂的生产。16S rRNA鉴定该菌株为溶淀粉孢菌RP2。菌株RP2对As5+、Fe2+、Mo6+、Pb2+、Co2+、Cu2+、Mn2+、Ni2+和Zn2+的耐受性至少为0.5 g/L。分离物乳化了几种有机底物，如航空涡轮燃料、柴油和二甲苯，乳化指数在60%以上。荧光显微镜显示，RP2菌株在培养14天内降解≥95%的模式碳氢化合物（正十六烷、柴油和煤油）。该生物表面活性剂富含六烯酸、十八烯酸和十八烯酸，在pH 6-10和20-60℃温度下稳定。NMR， FTIR和MALDI-TOF分析表明，生物表面活性剂主要是脂质和肽，但也含有少量碳水化合物残基，使该化合物更准确地描述为类脂肽，复合脂-糖-脂类或混合型生物表面活性剂。在临界胶束浓度为0.380 mg/mL时，生物表面活性剂可有效降低水的表面张力至29.56 mN/m。本研究首次报道了耐重金属溶淀粉酵母RP2菌株的生物表面活性剂特性和修复潜力。本研究结果初步表明，溶淀粉酵母RP2菌株具有对重金属的抗性和有效的修复能力，能够去除有机污染物；然而，对烷烃降解相关基因的研究和生物表面活性剂工业应用的进一步研究是必要的。

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引用次数: 0

Halotolerant plant growth promoting Streptomyces maritimus strain SST3 alleviates salinity stress in Solanum lycopersicum by improving growth, nutritional content and antioxidant enzymes 促进耐盐植物生长的海洋链霉菌SST3菌株通过改善茄的生长、营养成分和抗氧化酶来缓解盐胁迫

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-17 DOI: 10.1016/j.bcab.2025.103894

Sivagnanam Silambarasan , Pablo Cornejo , Cledir Santos , Cecilia García , Balu Kamaraj , Alisa S. Vangnai

Soil salinity is one of the most severe environmental stresses that hampers plant growth and crop yield globally. To overcome this, we isolated and characterized the potent halotolerant Streptomyces maritimus strain SST3 from marine sediment. A salt tolerance assay exhibited that strain SST3 could grow in the presence of NaCl up to 1200 mM. This actinobacterial strain showed beneficial plant growth-promoting (PGP) traits at different NaCl concentrations. Strain SST3 produced 5.73 mg mL⁻¹ of exopolysaccharides under 800 mM NaCl stress. The inoculation of strain SST3 under 50–200 mM NaCl stress conditions increased the root length (147–244 %), shoot length (96–226 %), fresh weight (82–303 %), dry weight (43–294 %) and chlorophyll contents (chlorophyll a 40–142 %, chlorophyll b 29–144 % and carotenoids 56–85 %) of Solanum lycopersicum as compared to corresponding uninoculated salt-stressed plants. Strain SST3 maintained a greater K⁺/Na⁺ ratio (0.21–2.14) while decreasing Na⁺ absorption (48–74 %), which mitigated the effects of salt stress on plant growth. The plants inoculated with strain SST3 showed a higher uptake of N (86–167 %), P (74–178 %), Ca (41–85 %), Mg (34–145 %) and Fe (46–125 %) in their leaves than the corresponding uninoculated salt-stressed plants. Furthermore, strain SST3-inoculated plants had reduced malondialdehyde concentrations (41–60 %) and increased catalase (28–147 %), superoxide dismutase (26–134 %) and ascorbate peroxidase (24–97 %) activities under salinity stress. Our findings suggest that utilizing the halotolerant strain SST3 can be an economical and sustainable method of alleviating salinity stress in crop production.

土壤盐碱化是影响全球植物生长和作物产量的最严重的环境压力之一。为了克服这一问题，我们从海洋沉积物中分离出了强耐盐的海洋链霉菌菌株SST3。耐盐试验表明，菌株SST3可以在高达1200 mM的NaCl环境下生长，该放线菌在不同NaCl浓度下表现出有益的植物生长促进（PGP）特性。菌株SST3在800 mM NaCl胁迫下产生5.73 mg mL−1的胞外多糖。在50 ~ 200 mM NaCl胁迫条件下接种菌株SST3后，茄根长（147 ~ 244%）、茎长（96 ~ 226%）、鲜重（82 ~ 303%）、干重（43 ~ 294%）和叶绿素含量（叶绿素a 40 ~ 142%、叶绿素b 29 ~ 144%、类胡萝卜素56 ~ 85%）较未接种盐胁迫植株显著增加。菌株SST3保持较高的K+/Na+比值（0.21-2.14），同时降低Na+吸收量（48 - 74%），减轻了盐胁迫对植株生长的影响。接种SST3菌株的植株叶片对N（86 ~ 167%）、P（74 ~ 178%）、Ca（41 ~ 85%）、Mg（34 ~ 145%）和Fe（46 ~ 125%）的吸收量高于未接种SST3菌株的植株。此外，sst3菌株接种植株在盐胁迫下丙二醛浓度降低（41% ~ 60%），过氧化氢酶（28 ~ 147%）、超氧化物歧化酶（26 ~ 134%）和抗坏血酸过氧化物酶（24 ~ 97%）活性升高。研究结果表明，利用耐盐菌株SST3是缓解作物生产中盐胁迫的一种经济、可持续的方法。

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引用次数: 0

Insecticidal activity of saline extract, protein fraction, and lectin (BmoLL) from Bauhinia monandra Kurz (Fabaceae) leaves on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Dryophthoridae) 紫荆（豆科）叶片生理盐水提取物、蛋白质组分和凝集素（BmoLL）对玉米象的杀虫活性（鞘翅目：干蛾科）

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-16 DOI: 10.1016/j.bcab.2025.103893

Thiago Lucas da Silva Lira , Cintia Ferreira de Almeida , Luis Afonso Silveira Farias , Ewelyn Cintya Felipe dos Santos , Magda Rhayanny Assunção Ferreira , Luiz Alberto Lira Soares , Thiago Henrique Napoleão , Patrícia Maria Guedes Paiva , Luana Cassandra Breitenbach Barroso Coelho

Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Dryophthoridae) is a major insect pest of stored maize grains, whose control relies on synthetic insecticides that pose environmental risks and promote resistance development. Plant-derived compounds, including secondary metabolites and lectins, are promising alternatives for pest management. In this study, the insecticidal effects of saline extract (SE), protein fraction (PF), and lectin (BmoLL) from Bauhinia monandra Kurz leaves on mortality, feeding, and nutritional parameters of S. zeamais adults were evaluated via incorporation into artificial diets. In vitro effects on trypsin, protease, α-amylase, and acetylcholinesterase activities were also assessed. Phytochemical analysis revealed flavonoids, cinnamic acid derivatives, saponins, and reducing sugars in SE and PF, with rutin confirmed by high-performance liquid chromatography (HPLC). BmoLL exhibited two bands (33 and 27 kDa) in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), confirming its presence in SE and PF and its successful purification. SE, PF, and BmoLL induced dose- and time-dependent mortality, with LC₅₀ values of 326.86, 119.50, and 7.00 mg/g at 7 days, and 227.39, 101.31, and 5.09 mg/g at 14 days, respectively. All samples caused feeding deterrence ranging from weak to strong and negatively affected nutritional parameters. SE and PF stimulated trypsin and protease activities, PF reduced α-amylase activity, whereas BmoLL increased it. SE and PF also enhanced acetylcholinesterase activity. In conclusion, SE, PF, and BmoLL exert lethal, antifeedant, antinutritional, and antimetabolic effects on S. zeamais, with BmoLL showing high efficacy at low concentrations, highlighting their potential as sustainable grain protection agents.

玉米象（Sitophilus zeamais Motschulsky, 1855）是贮藏玉米籽粒的主要害虫，其防治依赖于具有环境风险和促进抗性产生的合成杀虫剂。植物衍生化合物，包括次生代谢物和凝集素，是害虫管理的有希望的替代品。本研究通过将紫荆叶生理盐水提取物（SE）、蛋白质组分（PF）和凝集素（BmoLL）掺入人工饲料中，研究了其对玉米玉米螟成虫死亡率、摄食和营养参数的杀虫效果。对胰蛋白酶、蛋白酶、α-淀粉酶和乙酰胆碱酯酶活性的体外影响也进行了评估。植物化学分析表明，SE和PF中含有黄酮类化合物、肉桂酸衍生物、皂苷和还原糖，高效液相色谱法证实了芦丁的存在。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中，BmoLL显示出33和27 kDa的条带，证实其存在于SE和PF中，并且纯化成功。SE， PF和BmoLL诱导剂量和时间依赖性死亡率，LC50值在第7天分别为326.86,119.50和7.00 mg/g，第14天分别为227.39,101.31和5.09 mg/g。所有样本都引起了从弱到强的饲养威慑，并对营养参数产生了负面影响。SE和PF刺激胰蛋白酶和蛋白酶活性，PF降低α-淀粉酶活性，BmoLL提高α-淀粉酶活性。SE和PF均能增强乙酰胆碱酯酶活性。综上所述，SE、PF和BmoLL对玉米玉米具有致死性、拒食性、抗营养性和抗代谢作用，且BmoLL在低浓度条件下表现出较高的效果，具有作为可持续谷物保护剂的潜力。

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引用次数: 0

Exploring the advantages of Lactobacillus species from plants for future therapeutics and foods 探索植物乳酸菌在未来治疗和食品中的优势

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-13 DOI: 10.1016/j.bcab.2025.103889

Sureeporn Wichiansri , Surasak Siripornadulsil , Hironori Iwasaki , Wilailak Siripornadulsil

Lactic acid bacteria (LAB) are known for fermenting sugars into beneficial metabolites that promote host health, making them promising candidates for probiotic development. This study focused on isolating LAB from various plant sources, assessing their probiotic properties, evaluating safety features, and exploring their potential use in fruit-based probiotic products. Among 140 LAB isolates from various plant sources, 16 were classified into nine species, with 5 identified as potential probiotics: Lacticaseibacillus casei, Lacticaseibacillus paracasei, Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, and Lactococcus lactis. These isolates, along with commercial probiotics, exhibited multidrug-resistance (MDR) to certain antibiotics, particularly from the aminoglycoside, glycopeptide, and polyketide classes. Unprotected LAB cells demonstrated resistance to simulated gastric juice at pH 2 and effectively inhibited both gram-positive and gram-negative bacterial pathogens. Notably, three strains isolated from fruits and flowers: L. paracasei OR1, L. plantarum FL10, and L. rhamnosus MA3, survived in low-pH orange and coconut juices during cold storage, with a significant increase in viable cell counts after 24 h, indicating their ability to ferment sugars in natural fruit juices. L. paracasei OR1, derived from oranges, exhibited the highest viability and an MDR profile comparable to commercial strains. Whole-genome analysis of OR1 revealed genes associated with bacteriocin production, amino acid biosynthesis, and antioxidant activity, with no genes related to acquired antibiotic resistance. These findings suggest that OR1 could be cultivated in high-fructose fruit juices to produce viable probiotic cells and bioactive agents, with therapeutic potential. However, additional assessment is needed to confirm safety and prevent resistance gene transfer.

众所周知，乳酸菌（LAB）可以将糖发酵成有益的代谢物，促进宿主健康，这使它们成为益生菌开发的有希望的候选者。本研究的重点是从各种植物源中分离乳酸菌，评估其益生菌特性，评估其安全性，并探索其在水果益生菌产品中的潜在应用。从不同植物源分离的140株乳酸菌中，鉴定出9种16株，其中5株为潜在益生菌，分别为干酪乳杆菌、副干酪乳杆菌、植物乳杆菌、鼠李糖乳杆菌和乳酸球菌。这些分离菌与商业益生菌一起，对某些抗生素表现出多重耐药（MDR），特别是氨基糖苷类、糖肽类和聚酮类抗生素。未受保护的乳酸菌细胞对pH为2的模拟胃液表现出耐药性，并有效抑制革兰氏阳性和革兰氏阴性细菌病原体。值得注意的是，从水果和花朵中分离出的三株菌株：L. paracasei OR1、L. plantarum FL10和L. rhamnosus MA3在低ph的橙汁和椰子汁中冷藏后存活，24小时后活细胞计数显著增加，表明它们具有发酵天然果汁中糖的能力。从橙子中提取的副干酪乳杆菌OR1表现出最高的生存能力和与商业菌株相当的耐多药谱。OR1的全基因组分析发现了与细菌素产生、氨基酸生物合成和抗氧化活性相关的基因，没有与获得性抗生素耐药性相关的基因。这些发现表明OR1可以在高果糖果汁中培养，产生有活力的益生菌细胞和生物活性物质，具有治疗潜力。然而，需要进一步的评估来确认安全性并防止抗性基因转移。

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引用次数: 0

Selenite seedling priming enhances cadmium tolerance in Solanum lycopersicum cv. Micro-Tom 亚硒酸盐对茄幼苗镉耐受性的影响。Micro-Tom

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-13 DOI: 10.1016/j.bcab.2025.103887

Emilaine da Rocha Prado, Reginaldo de Oliveira, Mirela Vantini Checchio, Priscila Lupino Gratão

Cadmium (Cd) severely impacts crops as one of the most deleterious metals, whereas selenium (Se) enhances plant defense mechanisms under stress conditions. Seedling priming represents a promising pre-conditioning strategy to improve plant tolerance, but the effectiveness of different Se sources against Cd stress remains poorly understood. This study investigated the effects of Se priming in Solanum lycopersicum cv. Micro-Tom seedlings with selenate or selenite on Cd tolerance. Seedlings were treated with 10 μM selenate or selenite from 10 to 20 days after planting (DAP), and Cd stress (0.5 mM) was applied at 40 DAP. Growth, pigment contents, malondialdehyde (MDA), and hydrogen peroxide (H₂O₂) levels were evaluated. Cd stress reduced chlorophyll a, chlorophyll b, and carotenoids by 64.7 %, 56.6 %, and 54.8 %, respectively, and decreased leaf, stem, and root dry mass by 32.2 %, 52.9 %, and 30.0 %. Selenite priming mitigated these effects, reducing MDA by 87 % in leaves and 58 % in roots, while increasing leaf dry mass by 12 % and chlorophylls and carotenoids by up to 74 % under Cd stress. In contrast, selenate increased root dry mass but showed limited effectiveness in reducing oxidative stress markers. Principal component analysis (PCA) showed clear separation of treatments, with selenite + Cd clustering with tolerant responses and Cd-only positioned with stress indicators. This is the first study to demonstrate the efficacy of seedling priming with selenite (not selenate) in Micro-Tom tomato, highlighting its potential for improving crop tolerance to Cd stress.

镉（Cd）是对作物危害最大的金属之一，而硒（Se）能增强植物在逆境条件下的防御机制。幼苗启动是一种很有前途的预处理策略，可以提高植物的耐受性，但不同硒源对镉胁迫的有效性尚不清楚。研究了硒对茄类植物生长的影响。硒酸盐或亚硒酸盐对微tom幼苗的Cd耐受性。幼苗在播种后10 ~ 20天分别用10 μM硒酸盐或亚硒酸盐（DAP）处理，并在40 DAP下施加0.5 mM的Cd胁迫。评估生长、色素含量、丙二醛（MDA）和过氧化氢（H2O2）水平。Cd胁迫使叶绿素a、叶绿素b和类胡萝卜素分别降低64.7%、56.6%和54.8%，叶片、茎和根干质量分别降低32.2%、52.9%和30.0%。亚硒酸盐处理减轻了这些影响，在Cd胁迫下，叶片MDA减少87%，根系MDA减少58%，叶片干质量增加12%，叶绿素和类胡萝卜素增加74%。相比之下，硒酸盐增加了根干质量，但在降低氧化应激标志物方面效果有限。主成分分析（PCA）显示，亚硒酸盐+ Cd聚类具有耐受性，Cd仅定位于胁迫指标。该研究首次证实了亚硒酸盐（非硒酸盐）对微汤姆番茄幼苗的影响，强调了其提高作物对镉胁迫耐受性的潜力。

{"title":"Selenite seedling priming enhances cadmium tolerance in Solanum lycopersicum cv. Micro-Tom","authors":"Emilaine da Rocha Prado, Reginaldo de Oliveira, Mirela Vantini Checchio, Priscila Lupino Gratão","doi":"10.1016/j.bcab.2025.103887","DOIUrl":"10.1016/j.bcab.2025.103887","url":null,"abstract":"<div><div>Cadmium (Cd) severely impacts crops as one of the most deleterious metals, whereas selenium (Se) enhances plant defense mechanisms under stress conditions. Seedling priming represents a promising pre-conditioning strategy to improve plant tolerance, but the effectiveness of different Se sources against Cd stress remains poorly understood. This study investigated the effects of Se priming in <em>Solanum lycopersicum</em> cv. Micro-Tom seedlings with selenate or selenite on Cd tolerance. Seedlings were treated with 10 μM selenate or selenite from 10 to 20 days after planting (DAP), and Cd stress (0.5 mM) was applied at 40 DAP. Growth, pigment contents, malondialdehyde (MDA), and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels were evaluated. Cd stress reduced chlorophyll <em>a</em>, chlorophyll <em>b</em>, and carotenoids by 64.7 %, 56.6 %, and 54.8 %, respectively, and decreased leaf, stem, and root dry mass by 32.2 %, 52.9 %, and 30.0 %. Selenite priming mitigated these effects, reducing MDA by 87 % in leaves and 58 % in roots, while increasing leaf dry mass by 12 % and chlorophylls and carotenoids by up to 74 % under Cd stress. In contrast, selenate increased root dry mass but showed limited effectiveness in reducing oxidative stress markers. Principal component analysis (PCA) showed clear separation of treatments, with selenite + Cd clustering with tolerant responses and Cd-only positioned with stress indicators. This is the first study to demonstrate the efficacy of seedling priming with selenite (not selenate) in Micro-Tom tomato, highlighting its potential for improving crop tolerance to Cd stress.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103887"},"PeriodicalIF":3.8,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial deterioration of salt-cured hides: Impact of halophilic bacteria on protein integrity and sustainable leather processing 盐腌皮革的微生物劣化：嗜盐细菌对蛋白质完整性和可持续皮革加工的影响

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-13 DOI: 10.1016/j.bcab.2025.103890

Yasmin Khambhaty , Sugapriya Samidurai

The quality of animal hides and skins is adversely affected due to bacterial attack particularly during the preservation and soaking process. Owing to inadequate/inefficient preservation, the bacteria residing in the hides and skins cause damaging effects like stains, pinpricks, collagen degradation, uneven dyeing etc., which cannot be reversed, leading to production of substandard leathers. Sodium chloride (NaCl) is conventionally used for the curing of raw hides and skins in leather industries due to its perceived advantages over other preservation methods; nevertheless, the efficacy of salt preservation remains ambiguous. Salt-preserved animal hides and skins often develop unpleasant odour, loss of substance, grain damage, hair slip and pigmentation, which have been attributed to halophilic bacteria present in the salt. Hence, understanding the role of bacteria, both native to salts used for curing and those that thrive in salted hides and skins is crucial for addressing the damage caused to cured animal hides and skins and for improving the preservation methods. By integrating the knowledge about the specific bacteria involved in spoilage and the conditions that support their growth, it is possible to reduce waste, and improve the overall sustainability of hide and skin processing.

由于细菌的侵袭，特别是在保存和浸泡过程中，动物皮革的质量受到不利影响。由于保存不充分或效率低下，存在于皮革和皮肤中的细菌会造成污渍、针刺、胶原蛋白降解、染色不均匀等破坏性影响，这些影响无法逆转，导致生产不合格的皮革。氯化钠（NaCl）通常用于皮革工业中生皮的固化，因为它比其他保存方法有明显的优势；然而，盐保存的功效仍然不明确。盐保存的动物皮革和皮肤经常产生难闻的气味，物质损失，谷物损坏，头发脱落和色素沉着，这些都归因于盐中存在的嗜盐细菌。因此，了解细菌的作用，无论是用于腌制的盐的原生细菌，还是那些在盐渍皮革和皮肤中茁壮成长的细菌，对于解决对腌制动物皮革和皮肤造成的损害以及改进保存方法至关重要。通过整合涉及腐败的特定细菌的知识和支持它们生长的条件，有可能减少浪费，并提高皮革和皮肤加工的整体可持续性。

{"title":"Microbial deterioration of salt-cured hides: Impact of halophilic bacteria on protein integrity and sustainable leather processing","authors":"Yasmin Khambhaty , Sugapriya Samidurai","doi":"10.1016/j.bcab.2025.103890","DOIUrl":"10.1016/j.bcab.2025.103890","url":null,"abstract":"<div><div>The quality of animal hides and skins is adversely affected due to bacterial attack particularly during the preservation and soaking process. Owing to inadequate/inefficient preservation, the bacteria residing in the hides and skins cause damaging effects like stains, pinpricks, collagen degradation, uneven dyeing etc., which cannot be reversed, leading to production of substandard leathers. Sodium chloride (NaCl) is conventionally used for the curing of raw hides and skins in leather industries due to its perceived advantages over other preservation methods; nevertheless, the efficacy of salt preservation remains ambiguous. Salt-preserved animal hides and skins often develop unpleasant odour, loss of substance, grain damage, hair slip and pigmentation, which have been attributed to halophilic bacteria present in the salt. Hence, understanding the role of bacteria, both native to salts used for curing and those that thrive in salted hides and skins is crucial for addressing the damage caused to cured animal hides and skins and for improving the preservation methods. By integrating the knowledge about the specific bacteria involved in spoilage and the conditions that support their growth, it is possible to reduce waste, and improve the overall sustainability of hide and skin processing.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103890"},"PeriodicalIF":3.8,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145754137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal Inulinase: An insight into the unexplored dimension of multiproduct biorefineries 真菌菊粉酶：对多产品生物炼制未开发维度的洞察

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-10 DOI: 10.1016/j.bcab.2025.103886

Deepshikha , Pradeep Verma , Komal Agrawal

Global sustainability efforts increasingly focus on using renewable resources to efficiently develop bio-based products. In this context, the structural components of lignocellulosic materials have received significant attention. However, other naturally occurring polysaccharides also offer immense potential. Amongst which a reserved polysaccharide, inulin, holds significant importance as a renewable resource. It is a fructose-based polymer found in plants which is hydrolyzed by inulinase to generate intermediary precursor of industrial significance. These precursors exhibit bifunctional properties, serving as energy substrates and structural intermediates in product-driven biorefinery processes. The current review comprehensively discusses the ongoing research on inulinase while emphasizing towards its diverse microbial sources, catalytic mechanism and production enhancement techniques. The objective of the review is to critically analyze the advancements and role of inulinase towards bio-based product development and sustainability. Further, a comparative scientometric analysis of inulinase with various industrially important enzymes such as cellulase, laccase, xylanase, pectinase and hemi-cellulase has also been discussed. The findings of this study revealed the potential of including inulinase in multi-enzyme systems for complete bioconversion of renewable resources while improving the efficiency of bio-based production processes. In addition, the review highlights the future prospects of inulinase research and its potential in advancing sustainable biotechnological innovations.

全球可持续发展的努力日益集中于利用可再生资源高效开发生物基产品。在这种情况下，木质纤维素材料的结构成分受到了极大的关注。然而，其他天然存在的多糖也提供了巨大的潜力。其中一种保留的多糖菊粉作为一种可再生资源具有重要意义。它是一种在植物中发现的以果糖为基础的聚合物，通过菊粉酶水解产生具有工业意义的中间前体。这些前体具有双重功能，在产品驱动的生物炼制过程中作为能量底物和结构中间体。本文综述了菊粉酶的研究现状，重点介绍了菊粉酶的微生物来源、催化机理和提高产量的技术。综述的目的是批判性地分析菊粉酶在生物基产品开发和可持续性方面的进展和作用。此外，还讨论了菊粉酶与纤维素酶、漆酶、木聚糖酶、果胶酶和半纤维素酶等工业上重要的酶的比较科学计量分析。本研究的结果揭示了在多酶系统中加入菊粉酶的潜力，以完成可再生资源的生物转化，同时提高生物基生产过程的效率。此外，综述还强调了菊粉酶研究的未来前景及其在促进可持续生物技术创新方面的潜力。

{"title":"Fungal Inulinase: An insight into the unexplored dimension of multiproduct biorefineries","authors":"Deepshikha , Pradeep Verma , Komal Agrawal","doi":"10.1016/j.bcab.2025.103886","DOIUrl":"10.1016/j.bcab.2025.103886","url":null,"abstract":"<div><div>Global sustainability efforts increasingly focus on using renewable resources to efficiently develop bio-based products. In this context, the structural components of lignocellulosic materials have received significant attention. However, other naturally occurring polysaccharides also offer immense potential. Amongst which a reserved polysaccharide, inulin, holds significant importance as a renewable resource. It is a fructose-based polymer found in plants which is hydrolyzed by inulinase to generate intermediary precursor of industrial significance. These precursors exhibit bifunctional properties, serving as energy substrates and structural intermediates in product-driven biorefinery processes. The current review comprehensively discusses the ongoing research on inulinase while emphasizing towards its diverse microbial sources, catalytic mechanism and production enhancement techniques. The objective of the review is to critically analyze the advancements and role of inulinase towards bio-based product development and sustainability. Further, a comparative scientometric analysis of inulinase with various industrially important enzymes such as cellulase, laccase, xylanase, pectinase and hemi-cellulase has also been discussed. The findings of this study revealed the potential of including inulinase in multi-enzyme systems for complete bioconversion of renewable resources while improving the efficiency of bio-based production processes. In addition, the review highlights the future prospects of inulinase research and its potential in advancing sustainable biotechnological innovations.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103886"},"PeriodicalIF":3.8,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145754136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0