Biocatalysis and agricultural biotechnology最新文献_第6页

Impact of organic and conventional cropping systems on plant growth-promoting rhizobacteria (PGPR)

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103527

Trasmundi Flaviano , Molina Hernandez Junior Bernardo , Galieni Angelica , Campanelli Gabriele , Leteo Fabrizio , Polilli Walter , Platani Cristiano , Chaves-Lopez Clemencia , Stagnari Fabio

Maintaining soil health and productivity requires an understanding of soil biodiversity and the interactions within the soil microbiome and rhizosphere. This study focuses on Plant Growth-Promoting Rhizobacteria (PGPR) associated with horticultural crops (Solanaceae and Cucurbitaceae), grown under both conventional and organic no-till systems (within the MOnsampolo VEgetables Long Term Experiment - MOVE-LTE - established in 2001 by CREA-OF). We examined how these farming systems affect the PGPR population. Moreover, we investigated the ability of PGPR to induce indole-3-acetic acid (IAA) and siderophores production, phosphate solubilization, and antagonistic activity against Fusarium oxysporum and Alternaria alternata. A total of 147 bacterial isolates were tested; 28 identified strains with plant growth-promoting (PGP) traits were identified by sequencing the 16S rRNA gene, with their genomes matched to those in the NCBI database. Bacillus megaterium showed high siderophore units (90.55), P-solubilization Index (3.58), and antagonism activities. B. altitudinis and B. halotolerans were notable for high IAA production (46.23 and 40.7 ppm, respectively) while Paenarthrobacter nitroguajacolicus and Variovorax paradoxus exhibited high P-solubilization Index (3.97 and 4.39, respectively). Moreover, the distribution of strains with high relative abundance (>10%) among treatments reveal key characteristics of the PGPR community of the cropping systems: microbial community is significantly influenced by crop (tomato vs zucchini and pumpkin), with organic no-till management enhancing strain diversity more than abundance.

These results advance PGPR knowledge and indicate specific strengths and weaknesses around which consortia could be strategically developed to test performance under real conditions and ultimately improve crop yield and health across all agricultural systems.

{"title":"Impact of organic and conventional cropping systems on plant growth-promoting rhizobacteria (PGPR)","authors":"Trasmundi Flaviano , Molina Hernandez Junior Bernardo , Galieni Angelica , Campanelli Gabriele , Leteo Fabrizio , Polilli Walter , Platani Cristiano , Chaves-Lopez Clemencia , Stagnari Fabio","doi":"10.1016/j.bcab.2025.103527","DOIUrl":"10.1016/j.bcab.2025.103527","url":null,"abstract":"<div><div>Maintaining soil health and productivity requires an understanding of soil biodiversity and the interactions within the soil microbiome and rhizosphere. This study focuses on Plant Growth-Promoting Rhizobacteria (PGPR) associated with horticultural crops (<em>Solanaceae</em> and <em>Cucurbitaceae</em>), grown under both conventional and organic no-till systems (within the MOnsampolo VEgetables Long Term Experiment - MOVE-LTE - established in 2001 by CREA-OF). We examined how these farming systems affect the PGPR population. Moreover, we investigated the ability of PGPR to induce indole-3-acetic acid (IAA) and siderophores production, phosphate solubilization, and antagonistic activity against <em>Fusarium oxysporum</em> and <em>Alternaria alternata</em>. A total of 147 bacterial isolates were tested; 28 identified strains with plant growth-promoting (PGP) traits were identified by sequencing the 16S rRNA gene, with their genomes matched to those in the NCBI database. <em>Bacillus megaterium</em> showed high siderophore units (90.55), P-solubilization Index (3.58), and antagonism activities. <em>B. altitudinis</em> and <em>B. halotolerans</em> were notable for high IAA production (46.23 and 40.7 ppm, respectively) while <em>Paenarthrobacter nitroguajacolicus</em> and <em>Variovorax paradoxus</em> exhibited high P-solubilization Index (3.97 and 4.39, respectively). Moreover, the distribution of strains with high relative abundance (>10%) among treatments reveal key characteristics of the PGPR community of the cropping systems: microbial community is significantly influenced by crop (tomato <em>vs</em> zucchini and pumpkin), with organic no-till management enhancing strain diversity more than abundance.</div><div>These results advance PGPR knowledge and indicate specific strengths and weaknesses around which consortia could be strategically developed to test performance under real conditions and ultimately improve crop yield and health across all agricultural systems.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103527"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of procyanidins in early immature durian fruit using multi-platform metabolomics approach and their bioactivities

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103531

Putthamas Pewlong , Apinya Tubtimrattana , Pornkanok Pongpamorn , Sutthaorn Pothongsrisit , Nattaya Pattarapipatkul , Gholamreza Khaksar , Supakorn Potijun , Nat Tansrisawad , Atchara Paemanee , Manchumas Prousoontorn , Varisa Pongrakhananon , Supaart Sirikantaramas

Climate change and increasing population growth have led to a rise in food demand, resulting in greater agricultural waste. Furthermore, changing weather patterns damage crops, including durian, exacerbating this waste problem. In this study, we explore the potential of early immature durian fruit (ED) which is typically discarded during the thinning process to maintain fruit quality. Solvent extract optimization was implemented to prepare the ED extract, which was further analyzed for high-value compounds using a multi-platform metabolomics approach, incorporating UHPLC-ESI-QTOF-MS/MS and GC-MS techniques. Notably, we identified six bioactive secondary metabolites, consisting of procyanidin B2, procyanidin C1, (−)-epicatechin, quercitrin, quercetin-3-O-rutinoside and salipurposide. This research focuses on procyanidins to identify new production sources beyond grape seeds. Procyanidins were further quantified using HPLC. To evaluate their bioactivities, a crude extract was obtained from freeze-dried ED using 50% ethanol. The extract demonstrated strong anti-glycation and antioxidant activities, as confirmed by chemical assays. Additionally, it was observed that the extract enhanced the expression of enzymatic antioxidant genes (SOD, CAT, and GPX) in human keratinocyte cells (HaCaT) in vitro. This study highlights the potential of valorizing agricultural waste from ED as a novel source of procyanidins, which could be used in the nutraceutical, cosmetic, and pharmaceutical industries, thereby contributing to sustainability.

{"title":"Analysis of procyanidins in early immature durian fruit using multi-platform metabolomics approach and their bioactivities","authors":"Putthamas Pewlong , Apinya Tubtimrattana , Pornkanok Pongpamorn , Sutthaorn Pothongsrisit , Nattaya Pattarapipatkul , Gholamreza Khaksar , Supakorn Potijun , Nat Tansrisawad , Atchara Paemanee , Manchumas Prousoontorn , Varisa Pongrakhananon , Supaart Sirikantaramas","doi":"10.1016/j.bcab.2025.103531","DOIUrl":"10.1016/j.bcab.2025.103531","url":null,"abstract":"<div><div>Climate change and increasing population growth have led to a rise in food demand, resulting in greater agricultural waste. Furthermore, changing weather patterns damage crops, including durian, exacerbating this waste problem. In this study, we explore the potential of early immature durian fruit (ED) which is typically discarded during the thinning process to maintain fruit quality. Solvent extract optimization was implemented to prepare the ED extract, which was further analyzed for high-value compounds using a multi-platform metabolomics approach, incorporating UHPLC-ESI-QTOF-MS/MS and GC-MS techniques. Notably, we identified six bioactive secondary metabolites, consisting of procyanidin B2, procyanidin C1, (−)-epicatechin, quercitrin, quercetin-3-<em>O</em>-rutinoside and salipurposide. This research focuses on procyanidins to identify new production sources beyond grape seeds. Procyanidins were further quantified using HPLC. To evaluate their bioactivities, a crude extract was obtained from freeze-dried ED using 50% ethanol. The extract demonstrated strong anti-glycation and antioxidant activities, as confirmed by chemical assays. Additionally, it was observed that the extract enhanced the expression of enzymatic antioxidant genes (<em>SOD</em>, <em>CAT</em>, and <em>GPX</em>) in human keratinocyte cells (HaCaT) <em>in vitro</em>. This study highlights the potential of valorizing agricultural waste from ED as a novel source of procyanidins, which could be used in the nutraceutical, cosmetic, and pharmaceutical industries, thereby contributing to sustainability.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103531"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling a novel uronate dehydrogenase from industrial wastewater metagenomes for efficient galactaric acid production in engineered Saccharomyces cerevisiae

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103517

Piyada Bussadee , Nattapol Arunrattanamook , Ngoentra Samnaknit , Wuttichai Mhuantong , Phitsanu Pinmanee , Pattanop Kanokratana , Thidarat Nimchua , Xin-Qing Zhao , Verawat Champreda , Surisa Suwannarangsee

Galactaric acid is a valuable chemical utilized in the cosmetic and polymer industries. This platform chemical can be produced through a single enzymatic step catalyzed by uronate dehydrogenase (UDH) from D-galacturonic acid, which can be derived from the hydrolysis of pectin-rich feedstock. However, most characterized UDHs have been sourced from cultured microorganisms, leaving a significant gap in our understanding of UDHs from uncultured microbial communities. In this study, we identified and characterized three novel UDHs from the metagenomes of industrial wastewater. Following activity screening, the m2UDH was selected due to its superior enzyme activity in a Saccharomyces cerevisiae host. Through semi-rational protein engineering, the m2UDH^T123V variant was generated with 13-fold increase in enzyme activity compared to the wild-type enzyme. Engineered thermotolerant S. cerevisiae expressing m2UDH^T123V variants exhibited approximately 2.8-fold higher enzymatic activity and 2.4-fold higher galactaric acid production at 40 °C compared to the wild-type enzyme. Thus, this study highlights the significance of exploring diverse metagenomic environments for enzyme discovery and presents a promising approach for the bioprocessing of pectin-rich feedstocks into value-added biochemicals.

{"title":"Unveiling a novel uronate dehydrogenase from industrial wastewater metagenomes for efficient galactaric acid production in engineered Saccharomyces cerevisiae","authors":"Piyada Bussadee , Nattapol Arunrattanamook , Ngoentra Samnaknit , Wuttichai Mhuantong , Phitsanu Pinmanee , Pattanop Kanokratana , Thidarat Nimchua , Xin-Qing Zhao , Verawat Champreda , Surisa Suwannarangsee","doi":"10.1016/j.bcab.2025.103517","DOIUrl":"10.1016/j.bcab.2025.103517","url":null,"abstract":"<div><div>Galactaric acid is a valuable chemical utilized in the cosmetic and polymer industries. This platform chemical can be produced through a single enzymatic step catalyzed by uronate dehydrogenase (UDH) from D-galacturonic acid, which can be derived from the hydrolysis of pectin-rich feedstock. However, most characterized UDHs have been sourced from cultured microorganisms, leaving a significant gap in our understanding of UDHs from uncultured microbial communities. In this study, we identified and characterized three novel UDHs from the metagenomes of industrial wastewater. Following activity screening, the m2UDH was selected due to its superior enzyme activity in a <em>Saccharomyces cerevisiae</em> host. Through semi-rational protein engineering, the m2UDH<sup>T123V</sup> variant was generated with 13-fold increase in enzyme activity compared to the wild-type enzyme. Engineered thermotolerant <em>S. cerevisiae</em> expressing m2UDH<sup>T123V</sup> variants exhibited approximately 2.8-fold higher enzymatic activity and 2.4-fold higher galactaric acid production at 40 °C compared to the wild-type enzyme. Thus, this study highlights the significance of exploring diverse metagenomic environments for enzyme discovery and presents a promising approach for the bioprocessing of pectin-rich feedstocks into value-added biochemicals.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103517"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing starch-stabilized biogenic silver nanoparticles for sustainable bacterial blight management in soybean

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103509

Federico N. Spagnoletti , Irma N. Torres , Alejandra I. Hernandez , Romina Giacometti

Green nanotechnology is a new strategy that contributes to sustainable agriculture by enhancing crop production, restoring soil quality, and improving pest management control. Green nanotechnology has emerged as a promising strategy to overcome the limitations of conventional chemical synthesis of nanomaterials. In this study, biogenic silver nanoparticles (st-AgNPs) were synthesized in the presence of extracellular metabolites of the fungus Macrophomina phaseolina with further modification of the particle's corona using starch to enhance stability and performance. After confirming the biocidal activity of st-AgNPs, their potential use against bacterial blight caused by Pseudomonas savastanoi in soybean was analyzed. Under controlled conditions, the effects of different NP concentrations were tested on healthy and infected plants. No evidence of phytotoxicity was observed in plants treated with 50–400 μg ml⁻¹ of NPs. A 100 μg ml⁻¹ st-AgNPs dose was the most effective in controlling the disease progression. Subsequent greenhouse experiments showed that infected plants sprayed with st-AgNPs (100 μg ml⁻¹) improved their general status and increased the aerial biomass (36.6%) compared to non-treated plants. Furthermore, spray treatment with NPs partially reversed the negative effect of the infection, showing a 38.9% recovery in the greenness index. Notably, the severity of infection decreased by 78%, with no detectable silver traces in plant leaves. Biochemical analyses revealed that st-AgNPs treatment resulted in a reduction of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX) activities, as well as lipid peroxidation, compared to non-treated infected plants. These findings suggest that the developed st-AgNPs not only effectively control P. savastanoi infection in soybean but also exhibit the potential to serve as a low-impact, environmentally friendly tool for inclusion in plant disease management protocols across various crops.

{"title":"Harnessing starch-stabilized biogenic silver nanoparticles for sustainable bacterial blight management in soybean","authors":"Federico N. Spagnoletti , Irma N. Torres , Alejandra I. Hernandez , Romina Giacometti","doi":"10.1016/j.bcab.2025.103509","DOIUrl":"10.1016/j.bcab.2025.103509","url":null,"abstract":"<div><div>Green nanotechnology is a new strategy that contributes to sustainable agriculture by enhancing crop production, restoring soil quality, and improving pest management control. Green nanotechnology has emerged as a promising strategy to overcome the limitations of conventional chemical synthesis of nanomaterials. In this study, biogenic silver nanoparticles (st-AgNPs) were synthesized in the presence of extracellular metabolites of the fungus <em>Macrophomina phaseolina</em> with further modification of the particle's corona using starch to enhance stability and performance. After confirming the biocidal activity of st-AgNPs, their potential use against bacterial blight caused by <em>Pseudomonas savastanoi</em> in soybean was analyzed. Under controlled conditions, the effects of different NP concentrations were tested on healthy and infected plants. No evidence of phytotoxicity was observed in plants treated with 50–400 μg ml<sup>−1</sup> of NPs. A 100 μg ml<sup>−1</sup> st-AgNPs dose was the most effective in controlling the disease progression. Subsequent greenhouse experiments showed that infected plants sprayed with st-AgNPs (100 μg ml<sup>−1</sup>) improved their general status and increased the aerial biomass (36.6%) compared to non-treated plants. Furthermore, spray treatment with NPs partially reversed the negative effect of the infection, showing a 38.9% recovery in the greenness index. Notably, the severity of infection decreased by 78%, with no detectable silver traces in plant leaves. Biochemical analyses revealed that st-AgNPs treatment resulted in a reduction of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX) activities, as well as lipid peroxidation, compared to non-treated infected plants. These findings suggest that the developed st-AgNPs not only effectively control <em>P. savastanoi</em> infection in soybean but also exhibit the potential to serve as a low-impact, environmentally friendly tool for inclusion in plant disease management protocols across various crops.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103509"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trichoderma asperellum (T42)-mediated expression of CabHLH genes enhances nitrogen use efficiency and nutritional values of chickpea under salt and Fusarium wilt stresses

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103507

Nidhi Rai , Shashi Pandey Rai , Birinchi Kumar Sarma

Trichoderma asperellum T42 combats diverse phytopathogens and other stresses. However, its impact under combined stresses in modulating nutritional value and antioxidant properties in edible plant parts has not been thoroughly studied. Three chickpea cultivars, viz., wilt-resistant (JG-315), wilt-tolerant (JG-36), and wilt-susceptible (JG-62), were used to assess nutritional value and antioxidant contents under salt and pathogen (Foc)-challenged conditions. A sharp decrease in nodule numbers and biomass was observed in plants challenged with the combined stresses of Foc and salt in all three cultivars. However, seed treatment with T42 restored the nutritional value, enhanced antioxidant activities (1–2 folds) and increased total phenolic content (1.3–1.5 folds), protein (19–28%), proline, and micronutrients (7–28%) in chickpea seeds, particularly in the T42-treated plants subjected to the combined stress compared to the plants subjected to the combined stress without T42. The expression of two chickpea bHLH transcription factor genes, CabHLH114 and CabHLH115, associated with nodule development and nitrogen fixation, varied under different stresses. The genes were upregulated in T42-treated plants and correlated with the development of root nodules. The results thus suggest that Trichoderma-mediated expression of both nodulation-responsive genes led to the formation of healthy and functional nodules, which helped improve nitrogen use efficiency in the chickpea plants and contributed to the nutritional value of the chickpea seeds. The results highlighted that reduction in nutritional value due to environmental stresses could be restored in crop plants by applying potential bioagents such as T42 that restore nutritional quality and make the crops climate resilient.

{"title":"Trichoderma asperellum (T42)-mediated expression of CabHLH genes enhances nitrogen use efficiency and nutritional values of chickpea under salt and Fusarium wilt stresses","authors":"Nidhi Rai , Shashi Pandey Rai , Birinchi Kumar Sarma","doi":"10.1016/j.bcab.2025.103507","DOIUrl":"10.1016/j.bcab.2025.103507","url":null,"abstract":"<div><div><em>Trichoderma asperellum</em> T42 combats diverse phytopathogens and other stresses. However, its impact under combined stresses in modulating nutritional value and antioxidant properties in edible plant parts has not been thoroughly studied. Three chickpea cultivars, viz., wilt-resistant (JG-315), wilt-tolerant (JG-36), and wilt-susceptible (JG-62), were used to assess nutritional value and antioxidant contents under salt and pathogen (<em>Foc</em>)-challenged conditions. A sharp decrease in nodule numbers and biomass was observed in plants challenged with the combined stresses of <em>Foc</em> and salt in all three cultivars. However, seed treatment with T42 restored the nutritional value, enhanced antioxidant activities (1–2 folds) and increased total phenolic content (1.3–1.5 folds), protein (19–28%), proline, and micronutrients (7–28%) in chickpea seeds, particularly in the T42-treated plants subjected to the combined stress compared to the plants subjected to the combined stress without T42. The expression of two chickpea bHLH transcription factor genes, <em>CabHLH114</em> and <em>CabHLH115</em>, associated with nodule development and nitrogen fixation, varied under different stresses. The genes were upregulated in T42-treated plants and correlated with the development of root nodules. The results thus suggest that <em>Trichoderma</em>-mediated expression of both nodulation-responsive genes led to the formation of healthy and functional nodules, which helped improve nitrogen use efficiency in the chickpea plants and contributed to the nutritional value of the chickpea seeds. The results highlighted that reduction in nutritional value due to environmental stresses could be restored in crop plants by applying potential bioagents such as T42 that restore nutritional quality and make the crops climate resilient.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103507"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional profile, chemical composition and health promoting properties of Salicornia emerici Duval-Jouve and Sarcocornia alpini (Lag.) Rivas Mart. from southern Tunisia

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103502

Nesrine Harboub , Hedi Mighri , Naima Bennour , Mohamed Dbara , Catarina Pereira , Naima Chouikhi , Luísa Custódio , Raoudha Abdellaoui , Ahmed Akrout

The aerial biomass of the edible halophyte species Salicornia emerici Duval-Jouve and Sarcocornia alpini (Lag.) Rivas Mart. (Amaranthaceae), collected from southeastern Tunisia, was analyzed for its potential as a source of functional bioactive ingredients. For this purpose, the dried biomass was analyzed for its proximate and mineral composition, while its hydroethanolic extracts were evaluated for phenolic content by spectrophotometric and chromatographic techniques (HPLC-ESI-MS). The extracts were also assessed for in vitro antioxidant properties, including radical scavenging activity (RSA) against DPPH and ABTS^•+ radicals, as well as ferric reducing antioxidant power (FRAP). Furthermore, they were evaluated for in vitro cytotoxicity and enzymatic inhibition toward key enzymes related to human diseases, namely acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) (Alzheimer's disease: AD), α-amylase and α-glucosidase (type 2 Diabetes Mellitus: T2DM), and tyrosinase (skin hyperpigmentation disorders). The findings revealed that both halophytes contained significant levels of minerals, particularly sodium, potassium, and iron. The extracts were rich in bioactive phytochemicals, with naringin identified as the major compound in S. alpini, while quinic acid was predominant in S. emerici. Extracts from both species were non-toxic and demonstrated significant antioxidant properties. Neither species exhibited inhibitory activity toward cholinesterases; however, both showed strong α-glucosidase inhibition (>50%), with S. alpini also displaying superior tyrosinase inhibition activity (51%). These findings suggest that S. alpini and S. emerici hold promise as safe and sustainable sources of nutrients and natural compounds with antioxidant, anti-diabetic, and anti-tyrosinase properties. This potential could translate into significant economic benefits for the southeastern region of Tunisia.

{"title":"Nutritional profile, chemical composition and health promoting properties of Salicornia emerici Duval-Jouve and Sarcocornia alpini (Lag.) Rivas Mart. from southern Tunisia","authors":"Nesrine Harboub , Hedi Mighri , Naima Bennour , Mohamed Dbara , Catarina Pereira , Naima Chouikhi , Luísa Custódio , Raoudha Abdellaoui , Ahmed Akrout","doi":"10.1016/j.bcab.2025.103502","DOIUrl":"10.1016/j.bcab.2025.103502","url":null,"abstract":"<div><div>The aerial biomass of the edible halophyte species <em>Salicornia emerici</em> Duval-Jouve and <em>Sarcocornia alpini</em> (Lag.) Rivas Mart. (Amaranthaceae), collected from southeastern Tunisia, was analyzed for its potential as a source of functional bioactive ingredients. For this purpose, the dried biomass was analyzed for its proximate and mineral composition, while its hydroethanolic extracts were evaluated for phenolic content by spectrophotometric and chromatographic techniques (HPLC-ESI-MS). The extracts were also assessed for <em>in vitro</em> antioxidant properties, including radical scavenging activity (RSA) against DPPH and ABTS<sup>•+</sup> radicals, as well as ferric reducing antioxidant power (FRAP). Furthermore, they were evaluated for <em>in vitro</em> cytotoxicity and enzymatic inhibition toward key enzymes related to human diseases, namely acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) (Alzheimer's disease: AD), α-amylase and α-glucosidase (type 2 Diabetes Mellitus: T2DM), and tyrosinase (skin hyperpigmentation disorders). The findings revealed that both halophytes contained significant levels of minerals, particularly sodium, potassium, and iron. The extracts were rich in bioactive phytochemicals, with naringin identified as the major compound in <em>S. alpini</em>, while quinic acid was predominant in <em>S. emerici</em>. Extracts from both species were non-toxic and demonstrated significant antioxidant properties. Neither species exhibited inhibitory activity toward cholinesterases; however, both showed strong α-glucosidase inhibition (>50%), with <em>S. alpini</em> also displaying superior tyrosinase inhibition activity (51%). These findings suggest that <em>S. alpini</em> and <em>S. emerici</em> hold promise as safe and sustainable sources of nutrients and natural compounds with antioxidant, anti-diabetic, and anti-tyrosinase properties. This potential could translate into significant economic benefits for the southeastern region of Tunisia.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103502"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the wound healing potential of biocompatible nano-hydroxyapatite derived from parrotfish scale (Scarrus ghobban) waste for bone tissue engineering

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103493

Surya Parthasarathy , Palanisamy Arulselvan , Radha Gosala , Balakumar Subramanian

Fish waste management and the development of sustainable applications for fish byproducts have gained significant importance in recent times. This study focuses on repurposing discarded fish scales from Scarus ghobban to synthesize nano-hydroxyapatite (n-HAp) using an alkaline hydrolysis method. The n-HAp was comprehensively characterized through various analytical techniques, including FESEM, FT-IR, XRD, and TEM. The results revealed the successful production of n-HAp particles with an average size of 20–50 nm and the presence of functional groups such as esters, ethers, halogen compounds, and nitriles along with secondary amines, phenols, and alcohols which contribute to enhanced material characteristics. Additionally, through elemental mapping minerals such as Calcium, Phosphorous, Magnesium, and Sodium were confirmed. Cytotoxicity tests using L929 fibroblast cells demonstrated biocompatibility and wound healing assays indicated its potential for tissue regeneration. This approach not only harnesses valuable resources from fish waste but also holds promise for various biomedical applications, contributing to both economic growth and environmental sustainability. Ultimately, this nano-hydroxyapatite derived from fish scales showcases remarkable biocompatibility, positioning it as a promising candidate for the development of wound dressings.

{"title":"Exploring the wound healing potential of biocompatible nano-hydroxyapatite derived from parrotfish scale (Scarrus ghobban) waste for bone tissue engineering","authors":"Surya Parthasarathy , Palanisamy Arulselvan , Radha Gosala , Balakumar Subramanian","doi":"10.1016/j.bcab.2025.103493","DOIUrl":"10.1016/j.bcab.2025.103493","url":null,"abstract":"<div><div>Fish waste management and the development of sustainable applications for fish byproducts have gained significant importance in recent times. This study focuses on repurposing discarded fish scales from <em>Scarus ghobban</em> to synthesize nano-hydroxyapatite (n-HAp) using an alkaline hydrolysis method. The n-HAp was comprehensively characterized through various analytical techniques, including FESEM, FT-IR, XRD, and TEM. The results revealed the successful production of n-HAp particles with an average size of 20–50 nm and the presence of functional groups such as esters, ethers, halogen compounds, and nitriles along with secondary amines, phenols, and alcohols which contribute to enhanced material characteristics. Additionally, through elemental mapping minerals such as Calcium, Phosphorous, Magnesium, and Sodium were confirmed. Cytotoxicity tests using L929 fibroblast cells demonstrated biocompatibility and wound healing assays indicated its potential for tissue regeneration. This approach not only harnesses valuable resources from fish waste but also holds promise for various biomedical applications, contributing to both economic growth and environmental sustainability. Ultimately, this nano-hydroxyapatite derived from fish scales showcases remarkable biocompatibility, positioning it as a promising candidate for the development of wound dressings.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103493"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological valorization of yeast strains for lipids and carotenoids production from renewable resources

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103499

Andreísa Teixeira de Castro , Angélica Cristina de Souza , Cristina Ferreira Silva , Silvia Juliana Martinez , Rosane Freitas Schwan , Disney Ribeiro Dias

This study aimed to select yeast strains from the Agricultural Microbiology Culture Collection (CCMA) to produce lipids and/or carotenoids using crude glycerol and sugarcane molasses as alternative carbon sources. Among the selected strains, nine exhibited oleaginous characteristics, with Torulaspora maleeae (CCMA 0039) reaching the highest accumulation of lipid content (77.7%) in the glucose medium. Notably, the Exophiala spinifera (CCMA, 2073) strain exhibited 35% lipid accumulation in glycerol pure. Furthermore, Rhodotorula dairenensis (CCMA 945), Rhodotorula mucilaginosa (CCMA 0156), Rhodosporidium toruloides (CCMA, 2032) and Cystofilobasidium ferigula (CCMA 1623) were identified as carotenogenic. Crude glycerol has proven to be the most effective medium for lipogenesis and carotenogenesis, also expressing diversity in fatty acid profiles, with C. ferigula demonstrating promise in the coproduction of lipids and carotenoids, achieving 41.88% lipid accumulation and 2.76 μg/mL of total carotenoids. These findings highlight the potential for utilizing industrial by-products for the sustainable production of important metabolites, paving the way for their integration into industrial bioprocesses.

{"title":"Biotechnological valorization of yeast strains for lipids and carotenoids production from renewable resources","authors":"Andreísa Teixeira de Castro , Angélica Cristina de Souza , Cristina Ferreira Silva , Silvia Juliana Martinez , Rosane Freitas Schwan , Disney Ribeiro Dias","doi":"10.1016/j.bcab.2025.103499","DOIUrl":"10.1016/j.bcab.2025.103499","url":null,"abstract":"<div><div>This study aimed to select yeast strains from the Agricultural Microbiology Culture Collection (CCMA) to produce lipids and/or carotenoids using crude glycerol and sugarcane molasses as alternative carbon sources. Among the selected strains, nine exhibited oleaginous characteristics, with <em>Torulaspora maleeae</em> (CCMA 0039) reaching the highest accumulation of lipid content (77.7%) in the glucose medium. Notably, the <em>Exophiala spinifera</em> (CCMA, 2073) strain exhibited 35% lipid accumulation in glycerol pure. Furthermore, <em>Rhodotorula dairenensis</em> (CCMA 945), <em>Rhodotorula mucilaginosa</em> (CCMA 0156), <em>Rhodosporidium toruloides</em> (CCMA, 2032) and <em>Cystofilobasidium ferigula</em> (CCMA 1623) were identified as carotenogenic. Crude glycerol has proven to be the most effective medium for lipogenesis and carotenogenesis, also expressing diversity in fatty acid profiles, with <em>C. ferigula</em> demonstrating promise in the coproduction of lipids and carotenoids, achieving 41.88% lipid accumulation and 2.76 μg/mL of total carotenoids. These findings highlight the potential for utilizing industrial by-products for the sustainable production of important metabolites, paving the way for their integration into industrial bioprocesses.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103499"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging compost extract for heavy metal bioremediation and preserving the antioxidant activity of celery cabbage: A sustainable approach

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2024.103483

Mohammed Haroun , Ali Barham , Juanjuan Wang , Xiaoqing Qian

The study examined the efficiency of a combined bioadditive containing molasses with Bacillus firmus and Pseudomonas aeruginosa in the production of compost extract and their impact on the physiochemical, heavy metals bioremediation, and antioxidant activity in the plant. The methods involved aeration and a compost-to-water ratio of 1:10, following the CRD. The extract physiochemical parameters were measured. The extract heavy metals were determined according to ICP-MS (inductively coupled plasma-mass spectrometry). The activities of catalase, peroxidase, ascorbate peroxidase, and superoxide dismutase were determined using the methods of liquid portion. The results showed significant improvements in the pH from 5.5 to 8, Ec from 7.1 to 8.2, and O.M from 27.5% to 50%. The TN increased from 0.14 to 0.203 mg/kg, TP from 0.46 to 1.24 mg/kg, TK from 0.25 to 0.58 mg/kg, Ca from 20.3 to 25.11 mg/kg, and Mg from 10.75 to 7.75 mg/kg. Besides, the heavy metal bioremediation; Fe was lightly reduced from 8.3 to 7.4 mg/kg, Zn from 1.0 to 0.1 mg/kg, Cu from 0.1 to 0.01 mg/kg, and Cd from 0.1 to 0.02 mg/kg. CE significantly improved plant shoot and dry weight. The antioxidant activity of SOD and POD was significantly higher than APX and CAT. A positive correlation between physiochemical properties and heavy metals, while a negative correlation with antioxidant activity, specifically APX and POD. Beneficially, the application of CE enhances antioxidant enzyme activities, reduces heavy metal uptake in plants, and adopts healthier growth, thereby supporting sustainable agricultural practices.

{"title":"Emerging compost extract for heavy metal bioremediation and preserving the antioxidant activity of celery cabbage: A sustainable approach","authors":"Mohammed Haroun , Ali Barham , Juanjuan Wang , Xiaoqing Qian","doi":"10.1016/j.bcab.2024.103483","DOIUrl":"10.1016/j.bcab.2024.103483","url":null,"abstract":"<div><div>The study examined the efficiency of a combined bioadditive containing molasses with <em>Bacillus firmus</em> and <em>Pseudomonas aeruginosa</em> in the production of compost extract and their impact on the physiochemical, heavy metals bioremediation, and antioxidant activity in the plant. The methods involved aeration and a compost-to-water ratio of 1:10, following the CRD. The extract physiochemical parameters were measured. The extract heavy metals were determined according to ICP-MS (inductively coupled plasma-mass spectrometry). The activities of catalase, peroxidase, ascorbate peroxidase, and superoxide dismutase were determined using the methods of liquid portion. The results showed significant improvements in the pH from 5.5 to 8, Ec from 7.1 to 8.2, and O.M from 27.5% to 50%. The TN increased from 0.14 to 0.203 mg/kg, TP from 0.46 to 1.24 mg/kg, TK from 0.25 to 0.58 mg/kg, Ca from 20.3 to 25.11 mg/kg, and Mg from 10.75 to 7.75 mg/kg. Besides, the heavy metal bioremediation; Fe was lightly reduced from 8.3 to 7.4 mg/kg, Zn from 1.0 to 0.1 mg/kg, Cu from 0.1 to 0.01 mg/kg, and Cd from 0.1 to 0.02 mg/kg. CE significantly improved plant shoot and dry weight. The antioxidant activity of SOD and POD was significantly higher than APX and CAT. A positive correlation between physiochemical properties and heavy metals, while a negative correlation with antioxidant activity, specifically APX and POD. Beneficially, the application of CE enhances antioxidant enzyme activities, reduces heavy metal uptake in plants, and adopts healthier growth, thereby supporting sustainable agricultural practices.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103483"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas12a mediated rapid and efficient detection of Tomato Leaf Curl Karnataka Virus without amplification

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103528

Thalooru Shashikala , Dhawane Yogi , Kumar Akshay , Karuppannasamy Ashok , Somakalahalli Narasimhappa Nagesha , Maligeppagol Manamohan , Girish Kumar Jha , Ramasamy Asokan

Transboundary movement of agricultural produce increases the risk of introduction of plant pathogens into newer areas which pose a serious economic threat. This requires a quick, accurate detection method that will help in diagnosing the plant pathogens and make appropriate containment. The diagnostic methods thus developed will also be handy in the early screening for plant pathogens in the asymptomatic stage. Removing the infected plants in the early stage will help in maintaining the field stand to realize the full yield potential. Since vector-mediated transmission occurs at the seedling stage, random screening of the seedlings at the nursery stage will help in the clean planting programme. In this communication, we have compared the differential sensitivities of two methods viz. polymerase chain reaction and also CRISPR/Cas12a. In this regard, we have carried out whole genome sequencing for the ToLCKV isolate (PP763439.1) collected from tomatoes. We have expressed and purified Cas12a protein (187 kDa) and designed two guide RNAs (gRNAs) each for two genes viz. CP and Rep of ToLCKV and formed ribonucleoprotein (RNP) complex (sgRNA + Cas12a). Among the four RNP complexes tested, CPgRNA-1, CPgRNA-2 and REP gRNA1 enabled the detection of ToLCKV virus titre 10-fold lower (0.1 ng) than that of PCR assay (1.0 ng). Additionally, we also used CPgRNA-1 and CPgRNA-2 RNP to detect ToLCKV in field samples with high accuracy.

{"title":"CRISPR/Cas12a mediated rapid and efficient detection of Tomato Leaf Curl Karnataka Virus without amplification","authors":"Thalooru Shashikala , Dhawane Yogi , Kumar Akshay , Karuppannasamy Ashok , Somakalahalli Narasimhappa Nagesha , Maligeppagol Manamohan , Girish Kumar Jha , Ramasamy Asokan","doi":"10.1016/j.bcab.2025.103528","DOIUrl":"10.1016/j.bcab.2025.103528","url":null,"abstract":"<div><div>Transboundary movement of agricultural produce increases the risk of introduction of plant pathogens into newer areas which pose a serious economic threat. This requires a quick, accurate detection method that will help in diagnosing the plant pathogens and make appropriate containment. The diagnostic methods thus developed will also be handy in the early screening for plant pathogens in the asymptomatic stage. Removing the infected plants in the early stage will help in maintaining the field stand to realize the full yield potential. Since vector-mediated transmission occurs at the seedling stage, random screening of the seedlings at the nursery stage will help in the clean planting programme. In this communication, we have compared the differential sensitivities of two methods viz. polymerase chain reaction and also CRISPR/Cas12a. In this regard, we have carried out whole genome sequencing for the ToLCKV isolate (PP763439.1) collected from tomatoes. We have expressed and purified Cas12a protein (187 kDa) and designed two guide RNAs (gRNAs) each for two genes viz. <em>CP</em> and <em>Rep</em> of ToLCKV and formed ribonucleoprotein (RNP) complex (sgRNA + Cas12a). Among the four RNP complexes tested, CPgRNA-1, CPgRNA-2 and REP gRNA1 enabled the detection of ToLCKV virus titre 10-fold lower (0.1 ng) than that of PCR assay (1.0 ng). Additionally, we also used CPgRNA-1 and CPgRNA-2 RNP to detect ToLCKV in field samples with high accuracy.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103528"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0