Biocatalysis and agricultural biotechnology最新文献_第9页

Biodegradation of ibuprofen from Trichoderma pseudoasperelloides CBMAI 2752 and Penicillium sp. cf. guaibinense CBMAI 2758 isolated from amazonian soil 亚马逊土壤中假曲霉CBMAI 2752和绿皮青霉CBMAI 2758对布洛芬的生物降解研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-20 DOI: 10.1016/j.bcab.2025.103871

Beatriz L. Ferreira , Adriana M. Ferreira , Fabricio H. Holanda , Patrícia de A. Nóbrega , Thiago A.M. Brito , Josean F. Tavares , Willian G. Birolli , André Luiz M. Porto , Lilian Grace da S. Solon , Irlon M. Ferreira

Ibuprofen (IBU) is one of the most widely consumed non-steroidal anti-inflammatory drugs (NSAIDs) worldwide, and its improper disposal has raised significant concern for the World Health Organization. In recent years, several technologies have been developed as alternatives for managing pharmaceutical-derived organic micropollutants (OMPs). This study investigates the biodegradation and biotransformation of IBU mediated by whole-cells of fungi isolated from iron mine soils in the Amazon region. The strains Trichoderma pseudoasperelloides CBMAI 2752 and Penicillium sp. cf. guaibinense CBMAI 2758 were selected for biodegradation experiments using a three-variable Box-Behnken factorial design. Both strains achieved over 99 % IBU biodegradation under multiple conditions, with pH emerging as a key parameter influencing efficiency. The positive Pareto chart values (6.74 and 4.65) indicated that lower pH values enhanced the biodegradation process. Three potential metabolites were identified by LC–MS, including 2-(2-hydroxy-4-isobutylphenyl)propanoic acid. Toxicity assays with zebrafish embryos revealed that extracts obtained after IBU biodegradation and biotransformation were non-toxic at the tested concentrations, allowing normal embryonic development. In contrast, commercial IBU was classified as toxic, with no embryo formation observed. Overall, this research highlights the remarkable biotechnological potential of Amazonian soil fungi for the efficient degradation of IBU, offering an ecologically sustainable solution to mitigate pharmaceutical contamination in global aquatic environments.

布洛芬（IBU）是世界上使用最广泛的非甾体抗炎药（NSAIDs）之一，其处置不当引起了世界卫生组织的严重关注。近年来，已经开发了几种技术作为管理药物来源的有机微污染物（OMPs）的替代方案。本研究研究了从亚马逊地区铁矿土壤中分离的真菌全细胞介导的IBU的生物降解和转化。采用三变量Box-Behnken析因设计，选取假曲霉（Trichoderma pseudoasperelloides） CBMAI 2752和愈创木青霉（Penicillium sp. cf. guaibinense） CBMAI 2758进行生物降解试验。两株菌株在多种条件下均实现了99%以上的IBU生物降解，其中pH值成为影响效率的关键参数。帕累托图阳性值（6.74和4.65）表明，较低的pH值促进了生物降解过程。通过LC-MS鉴定出3种潜在代谢物，包括2-（2-羟基-4-异丁基苯基）丙酸。对斑马鱼胚胎的毒性试验表明，经过IBU生物降解和生物转化后获得的提取物在测试浓度下是无毒的，允许正常的胚胎发育。相比之下，商业IBU被归类为有毒，未观察到胚胎形成。总体而言，本研究突出了亚马逊土壤真菌在有效降解IBU方面的显著生物技术潜力，为减轻全球水生环境中的药物污染提供了生态可持续的解决方案。

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引用次数: 0

Immobilization and characteristics of Trichoderma longibrachiatum pectinase on periwinkle (Tympanotonos fuscatus) shell-derived chitosan support 长尾木霉果胶酶在长春花壳源壳聚糖载体上的固定化及特性研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-22 DOI: 10.1016/j.bcab.2025.103870

Chukwunonso Anthony Nsude , Arinze Linus Ezugwu , Samson Chinekwu Onoyima , Ozoemena Emmanuel Eje , Innocent Izuchukwu Ujah , Cosmas Ezekaibeya Achikanu , Moses Obinna Ezenwali , Lotanna Chidera Okwesili , Sabinus Oscar Onyebuchi Eze , Ikechukwu Noel Emmanuel Onwurah , Ferdinand Chiemeka Chilaka

Industrial applications of soluble pectinases face drawbacks due to losses in catalytic activity, downstream processing of products, and enzymes' non-recoverability. Immobilizing pectinases on chitosan support can circumvent these limitations and enhance the stability, recoverability, and reusability of the enzymes. This study focuses on immobilization of pectinase from Trichoderma longibrachiatum onto chitosan support. Crude pectinase extracted from submerged fermentation of mango pectin by T. longibrachiatum was partially purified via Sephadex G-200 gel filtration chromatography. Chitosan extracted by deacetylation of chitin from periwinkle (Tympanotonos fuscatus NCBI: txid496830) shells gave 66.79 ± 0.70 % yield, 76.00 ± 0.87 % solubility in 1 % acetic acid solution, and a degree of deacetylation (DDA) of 61.67 %. FTIR spectroscopic analysis revealed an absorption maximum at 1636.3 cm⁻¹, corresponding to the NH₂ bending of primary amine in chitosan. Partially purified pectinase was immobilized by adsorption on chitosan beads. Chitosan beads prepared in NaOH-methanol solution gave better immobilized pectinase activity (48.22 μmol/min) than those prepared in NaOH-ethanol solution (16.82 μmol/min) and were then adopted for bead formations. Maximal enzyme immobilization was achieved at contact pH (5.0) and time (3 h) with pectinase-specific activities of 0.55 ± 0.01 U/mg and 1.44 ± 0.06 U/mg, respectively. An immobilization efficiency (IE) of 80.12 % was achieved at optimal pH (5.0) and temperature (40^oC). The immobilized pectinase retained 57.19 ± 0.24 % of its original activity after three cycles of reuse. The results of this study revealed that the productions of pectinase and chitosan from locally sourced T. longibrachiatum and Tympanotonos fuscatus shells (respectively) are cost-effective and that the reusability of immobilized pectinase makes it robust for biotechnological applications.

可溶性果胶酶的工业应用面临着催化活性损失、产品下游加工和酶的不可恢复性等缺点。壳聚糖载体固定化果胶酶可以克服这些限制，提高酶的稳定性、可恢复性和可重复使用性。研究了壳聚糖载体固定化长尾木霉果胶酶的方法。采用Sephadex G-200凝胶过滤层析法对芒果果胶深层发酵中提取的粗果胶酶进行了部分纯化。以长春花（Tympanotonos fuscatus NCBI: txid496830）壳聚糖为原料，经去乙酰化提取壳聚糖的收率为66.79±0.70%，在1%醋酸溶液中的溶解度为76.00±0.87%，去乙酰化度（DDA）为61.67%。FTIR光谱分析表明，壳聚糖在1636.3 cm−1处的吸收最大值与壳聚糖中伯胺的NH2弯曲相对应。用壳聚糖吸附固定化部分纯化的果胶酶。甲醇溶液制备的壳聚糖微球比乙醇溶液（16.82 μmol/min）具有更好的固定化果胶酶活性（48.22 μmol/min），可用于壳聚糖微球的形成。在接触pH（5.0）和接触时间（3 h）下，固定化效果最佳，果胶酶特异性活性分别为0.55±0.01 U/mg和1.44±0.06 U/mg。在最佳pH（5.0）和温度（40℃）条件下，固定化效率（IE）为80.12%。经3次循环使用后，固定化果胶酶的活性保持了57.19±0.24%。本研究结果表明，利用当地的长尾竹和褐斑鼓壳分别生产果胶酶和壳聚糖具有成本效益，并且固定化果胶酶的可重复使用性使其具有良好的生物技术应用前景。

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引用次数: 0

Coffee fermentation using polyethylene bags as a bioreactor: effect of adding coffee peels and a starter culture 用聚乙烯袋作为生物反应器的咖啡发酵：添加咖啡皮和发酵剂的效果

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-27 DOI: 10.1016/j.bcab.2025.103869

Antônio Sergio Souza , José Maria Rodrigues da Luz , Fabrícia Queiroz Mendes , Willian dos Santos Gomes , Lucas Louzada Pereira , Paulo Sérgio Monteiro , Evandro Galvão Tavares Menezes , André Mundstock Xavier de Carvalho

Due to the popularization of specialty coffees, producers began to invest in the development of post-harvest processing techniques to improve the chemical and sensory characteristics of the coffee beverage. Thus, the objective of this study was to evaluate the effect of using coffee peels and a starter culture of Saccharomyces cerevisiae as inocula in coffee fermentation, conducted in polyethylene bags as a bioreactor, on the microbiological, physical, chemical, and sensory parameters of the coffee. The use of coffee peels as an inoculum source and the choice of this yeast were based on our previous studies. Peeled coffee cherries without or with the addition of peels were used in spontaneous and induced fermentations by S. cerevisiae at 30 °C Fermentation times were 24, 48, 72, 96, and 120 h. A significant yeast colony count after 120 h of fermentation was observed only in the musts from S. cerevisiae-induced fermentations. The addition of the coffee bark also did not influence the viable microbial cell count. A reduction in pH, Brix, and average temperature values was observed in the wort during fermentation. The bands in the infrared spectra in the regions of 1317 and 1095 cm⁻¹ and 1093 and 873 cm⁻¹ showed the chemical differences between the treatments after 120 h of fermentation. S. cerevisiae-induced fermentations had a greater contribution to the sensory perceptions of the coffee beverage than spontaneous fermentation. Thus, fermentation efficiency was directly proportional to fermentation time, anaerobic environment, and inoculum source (endogenous coffee cherry or starter culture).

由于精品咖啡的普及，生产商开始投资开发采收后的加工技术，以提高咖啡饮料的化学和感官特性。因此，本研究的目的是评估使用咖啡皮和酿酒酵母发酵剂作为接种剂，在聚乙烯袋中作为生物反应器进行咖啡发酵，对咖啡的微生物，物理，化学和感官参数的影响。使用咖啡皮作为接种源和酵母的选择是基于我们之前的研究。在30℃条件下，将去皮的咖啡樱桃与不加果皮的咖啡樱桃分别进行自发发酵和诱导发酵，发酵时间分别为24、48、72、96和120 h。发酵120 h后，仅在酿酒酵母诱导发酵的酵母菌中观察到显著的酵母菌落计数。咖啡皮的添加也不影响活菌细胞数。在发酵过程中观察到麦芽汁的pH值、糖度和平均温度值的降低。发酵120 h后，1317和1095 cm−1以及1093和873 cm−1区域的红外光谱显示了不同处理之间的化学差异。酿酒酵母诱导的发酵比自发发酵对咖啡饮料的感官知觉有更大的贡献。因此，发酵效率与发酵时间、厌氧环境和接种物来源（内源性咖啡樱桃或发酵剂）成正比。

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引用次数: 0

Dose-dependent effects of Bacillus subtilis SB008 on root development, photosynthetic efficiency, and antioxidant activity in soybean plants 枯草芽孢杆菌SB008对大豆根系发育、光合效率和抗氧化活性的剂量依赖性影响

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-12-02 DOI: 10.1016/j.bcab.2025.103878

João Arthur dos Santos Oliveira , Erika Fritegotto Leite , Giovana Gomes Ferreira Matos , Natieli Jenifer Mateus Corniani , Carolina Fedrigo Coneglian , Maria Eduarda de Souza Rocha , Patricia Viero , Luana Thais Varize Marcusso , Carlos Augusto Corniani da Silva , Willian Martire Marcusso

This study demonstrates the biotechnological potential of the endophytic bacterium Bacillus subtilis SB008 as a multifunctional bioinoculant for sustainable agriculture. The strain produced key metabolites, including indole-3-acetic acid (3.3 μg/mL), exopolysaccharides (361 μg/mL), siderophores (75.7 %), and amino acids, alongside hydrolytic enzymes (amylase, cellulase, protease, and chitinase). Polynomial regression modeling revealed dose-dependent effects: seed inoculation (1 mL/kg) enhanced root system development, while single foliar application (0.220 L/ha) enhanced chlorophyll accumulation. Notably, foliar treatment at 0.160 L/ha elicited a robust antioxidant response, simultaneously increasing catalase (1354 nmol/min/μg protein) and superoxide dismutase (3075 nmol/min/μg protein) activities while maintaining balanced peroxidase levels (7715 nmol/min/μg protein). These findings suggest that SB008 functions as a dual-purpose bioinoculant, enhancing plant development through morphological effects on root architecture and biochemical modulation of photosynthesis and antioxidant enzymes. Its compatibility with soybean cultivation suggests promise for integration into sustainable management systems.

该研究证明了内生细菌枯草芽孢杆菌SB008作为可持续农业多功能生物接种剂的生物技术潜力。该菌株主要代谢产物包括吲哚-3-乙酸（3.3 μg/mL）、胞外多糖（361 μg/mL）、铁载体（75.7%）和氨基酸，以及水解酶（淀粉酶、纤维素酶、蛋白酶和几丁质酶）。多项式回归模型显示剂量依赖性效应：种子接种（1 mL/kg）促进根系发育，单叶接种（0.220 L/ha）促进叶绿素积累。值得注意的是，叶面浓度为0.160 L/ha的处理引起了强烈的抗氧化反应，同时增加了过氧化氢酶（1354 nmol/min/μg蛋白）和超氧化物歧化酶（3075 nmol/min/μg蛋白）活性，同时保持了平衡的过氧化物酶水平（7715 nmol/min/μg蛋白）。上述结果表明，SB008作为一种具有双重作用的生物接种剂，通过对根系形态的影响以及光合作用和抗氧化酶的生化调节来促进植物发育。它与大豆种植的兼容性表明，它有望纳入可持续管理系统。

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引用次数: 0

Optimization of the extraction of phenolic compounds from red grape potsuit aimed at inhibiting Botrytis cinerea in vitro 体外抑制葡萄灰霉病的酚类化合物提取工艺优化

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-15 DOI: 10.1016/j.bcab.2025.103858

Larissa Fernanda Finazzi da Costa , Larissa Perreira Machado , Sthefanie Lemos Barbosa , Sabrina Carra , Eloane Malvessi

Red grape pomace, a byproduct of wine production, has significant levels of phenolic compounds with biological activity, including antifungal characteristics. The recovery of these compounds facilitates the formulation of natural fungicidal compositions that adhere to circular economy principles. The goal of this study was to optimize the extraction of phenolic compounds from the red grape pomace of the Vitis vinifera varieties Merlot and Pinot noir, as well as to evaluate their in vitro antifungal efficacy against Botrytis cinerea. Initial studies were performed to assess the extraction parameters: solvent type, temperature, and temperature. After these were determined, additional parameters (solvent concentration, agitation, and biomass percentage) were enhanced using a central composite rotary design (CCRD). Ethanol was discovered to be the most effective solvent, delivering up to 151.06 g GAE·100 g⁻¹ under ideal conditions (50 % v/v ethanol, 30 % w/v pomace, 700 rpm, 50 °C, 2 h). Merlot and Pinot Noir extracts show significant fungicidal effects, limiting B. cinerea growth in vitro through contact and volatile action, as well as lowering the production of extracellular enzymes associated with fungal pathogenicity (amylase, cellulase, and esterase). These findings show the feasibility of producing bioactive extracts from winemaking residues as long-term alternatives to chemical fungicides. Future research should concentrate on process scalability, compound stability, and field validation to encourage commercial applications and make larger contributions to SDGs 12 and 13.

红葡萄渣是葡萄酒生产的副产品，含有大量具有生物活性的酚类化合物，包括抗真菌特性。这些化合物的回收有利于符合循环经济原则的天然杀菌剂组合物的配方。以葡萄品种梅洛（Merlot）和黑皮诺（Pinot noir）为原料，对红葡萄渣中酚类化合物的提取工艺进行了优化，并对其体外抗葡萄灰霉病（Botrytis cinerea）的效果进行了评价。进行初步研究以评估提取参数：溶剂类型，温度和温度。在确定这些参数后，使用中心复合旋转设计（CCRD）增强其他参数（溶剂浓度，搅拌和生物量百分比）。发现乙醇是最有效的溶剂，在理想条件下（50% v/v乙醇，30% w/v渣油，700转/分钟，50°C， 2小时），最高可达151.06 g GAE·100 g−1。梅洛和黑皮诺提取物显示出显著的杀真菌作用，通过接触和挥发作用限制了灰葡萄球菌在体外的生长，并降低了与真菌致病性相关的细胞外酶（淀粉酶、纤维素酶和酯酶）的产生。这些发现表明，从酿酒残留物中生产生物活性提取物作为化学杀菌剂的长期替代品是可行的。未来的研究应侧重于工艺可扩展性、化合物稳定性和现场验证，以鼓励商业应用，并为可持续发展目标12和13做出更大的贡献。

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引用次数: 0

Optimizing xanthan gum production using okara and response surface methodology: Unlocking sustainable biopolymer production 利用黄原胶和响应面方法优化黄原胶生产：解锁可持续生物聚合物生产

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-11 DOI: 10.1016/j.bcab.2025.103852

Richard Vincent Asase, Tatiana Vladimirovna Glukhareva

Xanthan gum is a commercially important exopolysaccharide with production influenced by raw materials costs and nutrient availability. This study optimized okara, glucose and KH₂PO₄ concentrations for xanthan gum production by Xanthomonas campestris B6720 using response surface methodology combined with a desirability function. A maximum yield of 29.137 g/L was achieved in a confirmatory run under the optimized conditions of 10, 0.2, and 2 g/100 ml for okara, KH₂PO₄, and glucose respectively. Structural characterization using FTIR, ¹H NMR, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) compared xanthan gum produced from okara (OKAXG), standard media (STDXG), and a commercial source (COMXG). Principal component analysis (PCA) of FTIR data revealed distinct structural differences, with OKAXG being unique. TGA showed initial mass losses of approximately 12 % water evaporation, followed by major degradation mass losses of 38.14 % (COMXG), 53.49 % (STDXG), and 45.06 % (OKAXG). Derivative thermogravimetry indicated sharper decomposition peaks for COMXG and STDXG and broader peaks for OKAXG, suggesting enhanced thermal stability. Elemental analysis showed significant differences (p < 0.05) in carbon, hydrogen, and nitrogen contents among the xanthan gum samples except for hydrogen between STDXG and COMXG. Viscosity measurements showed lower viscosity for STDXG and OKAXG than for COMXG. Antioxidant activity by DPPH scavenging was highest in COMXG (65 %), followed by OKAXG (55 %) and STDXG (48 %). These findings indicate that the production substrate influences xanthan gum's molecular structure, thermal behavior, and bioactivity. Okara as an alternative nutrient source offers a promising, sustainable, and cost-effective approach to xanthan production by valorizing agro-industrial by-products.

黄原胶是一种重要的商业外多糖，其生产受原料成本和养分利用率的影响。本研究利用响应面法结合期望函数优化了油菜黄单胞菌B6720生产黄原胶所需的okara、葡萄糖和KH2PO4浓度。在10、0.2和2 g/100 ml的优化条件下，对菜心、KH2PO4和葡萄糖进行验证，产率最高可达29.137 g/L。利用FTIR、1H NMR、热重分析（TGA）和差示扫描量热法（DSC）对由okara （OKAXG）、标准培养基（STDXG）和商业来源（COMXG）生产的黄原胶进行了结构表征。主成分分析（PCA）显示出明显的结构差异，其中OKAXG是唯一的。TGA显示初始质量损失约为12%，其次是主要降解质量损失38.14% (COMXG), 53.49% （STDXG）和45.06% （OKAXG）。导数热重分析表明，COMXG和STDXG的分解峰更陡，OKAXG的分解峰更宽，表明其热稳定性增强。元素分析表明，除氢外，STDXG和COMXG黄原胶样品的碳、氢、氮含量差异显著（p < 0.05）。粘度测量表明，STDXG和OKAXG的粘度低于COMXG。COMXG对DPPH的清除活性最高（65%），其次是OKAXG（55%）和STDXG（48%）。这些发现表明，生产底物影响黄原胶的分子结构、热行为和生物活性。Okara作为一种替代营养来源提供了一个有前途的，可持续的，具有成本效益的方法黄原胶生产的农业工业副产品。

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引用次数: 0

Bacillus licheniformis inoculation accelerates the humification process through regulating the shikimic acid pathway and lignin degradation in pilot-scale composting 地衣芽孢杆菌接种通过调节莽草酸途径和木质素降解加速中试堆肥腐殖质化过程

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-13 DOI: 10.1016/j.bcab.2025.103859

Rixin Bao, Xiaolin Lu, Wenyu Huang, Qunliang Li

Lignin degradation (LD) and shikimic acid pathway (SAP) are important for the synthesis of humus precursor. This pilot-scale study investigated the regulatory role of Bacillus licheniformis (TA65) on these pathways and the humification process. Results demonstrated that TA65 inoculation significantly accelerated the LD rate, with the experimental group (CT) achieving a degradation rate of 35.60 %, substantially higher than that of the control (CK, 26.80 %). Compared with CK, CT raised humus substances (HS) and humic acid (HA) contents by 20.72 % and 170.01 %, respectively. These enhancements were driven by a notable increase in key enzyme activities; peak polyphenol oxidase activity and laccase activity in CT reached 14.20 U/g and 950.78 U/g, respectively, both significantly higher than in CK. Moreover, microbial analysis revealed that TA65 inoculation reshaped the humification-associated microbial community, significantly elevating the relative abundances of Saccharomonospora and Planifilum. Concurrently, CT upregulated a suite of SAP-related genes (including aroC, aroF/H, aroG/A, aroK/L). Furthermore, correlation analysis revealed strong positive correlations among LD, the relative abundance of SAP-related genes, and humification parameters. These integrated metabolic regulations reduced fulvic acid (FA), promoted the accumulation of HS and HA during composting. The findings confirm that TA65 enhances humus synthesis primarily by altering the compost microenvironment and improving the metabolic functions of key microbes, which provides a theoretical foundation for the targeted enhancement of humification in large-scale composting.

木质素降解（LD）和莽草酸途径（SAP）是腐殖质前体合成的重要途径。本中试研究探讨了地衣芽孢杆菌（Bacillus licheniformis, TA65）对这些途径和腐殖化过程的调控作用。结果表明，接种TA65显著提高了降解率，试验组（CT）降解率达到35.60%，显著高于对照（CK）的26.80%。与对照相比，处理后腐殖质（HS）和腐殖酸（HA）含量分别提高了20.72%和170.01%。这些增强是由关键酶活性的显著增加所驱动的；多酚氧化酶和漆酶活性峰值分别达到14.20 U/g和950.78 U/g，均显著高于对照。此外，微生物分析显示，TA65接种重塑了腐殖化相关微生物群落，显著提高了Saccharomonospora和Planifilum的相对丰度。同时，CT上调一系列sap相关基因（包括aroC、aroF/H、aroG/ a、aroK/L）。此外，相关分析显示，LD与sap相关基因的相对丰度和腐殖化参数之间存在较强的正相关。这些综合代谢调控在堆肥过程中减少了黄腐酸（FA），促进了HS和HA的积累。研究结果证实，TA65主要通过改变堆肥微环境和改善关键微生物代谢功能来促进腐殖质合成，为大规模堆肥中定向增强腐殖质化提供了理论基础。

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引用次数: 0

Enhancing β-carotene and biomass production in Blakeslea trispora using Plackett–Burman design screening and predictive modeling 利用Plackett-Burman设计筛选和预测模型提高三孢子黑藻β-胡萝卜素和生物量产量

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-04 DOI: 10.1016/j.bcab.2025.103845

Negin Ghazanfari , Ali Motlagh Hosseini , Morteza Taki , Fereshteh Falah , Behrooz Alizadeh Behbahani , Farideh Tabatabaei Yazdi , Alireza Vasiee

Blakeslea trispora is a filamentous fungus known for producing β-carotene, especially in the all-trans form, a powerful antioxidant and precursor to vitamin A. This study aims to identify and evaluate key factors influencing β-carotene and biomass production using the Plackett–Burman design. Seven variables were tested, including concentrations of dairy sludge, soybean meal, yeast extract, incubation time, temperature, and shaker speed. Among these, soybean meal, dairy sludge, and yeast extract were identified as the most significant contributors to enhanced β-carotene yield and biomass accumulation. These substrates not only offer nutritional value but also present a low-cost and sustainable alternative for culture medium formulation. In the second phase, a Gaussian Process Regression (GPR) model was applied to predict β-carotene concentration and biomass yield based on the selected input parameters. The model was validated using k-fold cross-validation, which showed minimal standard deviation in prediction errors and demonstrated strong predictive performance. The Mean Absolute Percentage Error (MAPE) for biomass and β-carotene prediction was 1.82 % and 4.39 %, respectively. These findings support the combined use of experimental design and machine learning techniques for optimizing fungal fermentation processes in β-carotene production.

Blakeslea trispora是一种丝状真菌，以生产β-胡萝卜素而闻名，特别是全反式，一种强大的抗氧化剂和维生素a的前体。本研究旨在利用Plackett-Burman设计确定和评估影响β-胡萝卜素和生物量生产的关键因素。测试了7个变量，包括乳污泥、豆粕、酵母提取物、孵育时间、温度和摇床速度的浓度。其中，豆粕、乳污泥和酵母提取物被认为是提高β-胡萝卜素产量和生物量积累的最显著贡献者。这些基质不仅具有营养价值，而且还提供了一种低成本和可持续的培养基配方替代方案。在第二阶段，基于选择的输入参数，应用高斯过程回归（GPR）模型预测β-胡萝卜素浓度和生物量产量。采用k-fold交叉验证对模型进行验证，预测误差标准差最小，预测效果较好。生物量和β-胡萝卜素预测的平均绝对百分比误差（MAPE）分别为1.82%和4.39%。这些发现支持结合实验设计和机器学习技术来优化真菌发酵过程中β-胡萝卜素的生产。

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引用次数: 0

Biocatalytic degradation of fluoroquinolone antibiotics in wastewater using laccase immobilized on spent mushroom waste biochar 蘑菇废生物炭固定化漆酶降解废水中的氟喹诺酮类抗生素

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-10-27 DOI: 10.1016/j.bcab.2025.103834

Anamika Ghose , Debaditya Gupta , Veswuh Nuzelu , Latha Rangan , Sudip Mitra

The present study highlights the effective biodegradation of two fluoroquinolone antibiotics, ciprofloxacin and norfloxacin commonly detected in surface water, river water, and treated water through the application of laccase immobilized on raw biochar derived from spent mushroom waste (SMW) of Pleurotus florida. The immobilized enzyme system demonstrated high degradation efficiencies of 96.44 % and 93.22 % for ciprofloxacin and norfloxacin, respectively, within a time of 3 h, as quantified by high-performance liquid chromatography (HPLC). The enhanced biodegradation efficiency is closely linked to improved immobilization efficiency, which contributes to greater enzyme stability and activity. To achieve this, key process parameters such as pH (3–6), temperature (30–50 °C), and enzyme dosage (1–5 U g⁻¹) were evaluated using Box-Behnken Design (BBD) and Box-Behnken Design coupled with Artificial Neural Network (BBD-ANN). The approach accurately predicted a maximum adsorptive immobilization efficiency of 65.03 % at pH 4.5, 30 °C and 5 U g⁻¹ enzyme dosage, validating the experimental data. Compared to free laccase, the immobilized system exhibited enhanced stability across a wide pH range (3–10), higher temperatures (20–75 °C), and in the presence of metal ions and inhibitors. Further, the environmental safety of the degradation process was assessed by performing algal toxicity test using Scenedesmus abundans showing reduced toxicity in the degraded products, confirming the non-toxic nature of the degraded products. Nevertheless, this study offers a practical and sustainable strategy for antibiotic remediation in wastewater, emphasizing the conjunction of waste-derived materials with enzyme-based biocatalysis to advance circular bioeconomy approaches and improve water treatment technologies.

本研究通过将漆酶固定化在佛罗里达平菇（Pleurotus florida）废蘑菇（SMW）生炭上，对地表水、河水和处理水中常见的两种氟喹诺酮类抗生素环丙沙星和诺氟沙星进行了有效的生物降解。经高效液相色谱法测定，固定化酶体系对环丙沙星和诺氟沙星的降解效率分别为96.44%和93.22%，时间为3 h。生物降解效率的提高与固定化效率的提高密切相关，固定化效率有助于提高酶的稳定性和活性。为了实现这一目标，使用Box-Behnken设计（BBD）和Box-Behnken设计结合人工神经网络（BBD- ann）对关键工艺参数如pH（3-6）、温度（30-50°C）和酶用量（1 - 5 U g−1）进行了评估。该方法准确预测了在pH 4.5, 30°C， 5 U g−1酶用量下的最大吸附固定效率为65.03%，验证了实验数据。与游离漆酶相比，固定化体系在较宽的pH范围（3-10）、较高的温度（20-75°C）以及金属离子和抑制剂存在下都表现出更高的稳定性。此外，通过使用Scenedesmus abundance进行藻类毒性测试，对降解过程的环境安全性进行了评估，结果显示降解产物的毒性降低，证实了降解产物的无毒性。尽管如此，本研究为废水中的抗生素修复提供了一个实用和可持续的策略，强调将废物衍生材料与酶基生物催化相结合，以推进循环生物经济方法和改进水处理技术。

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引用次数: 0

Himalayan botanical mediated elicitation regulates wheat growth through secondary metabolites and hormonal signalling 喜马拉雅植物介导的诱导通过次生代谢物和激素信号调节小麦生长

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-07 DOI: 10.1016/j.bcab.2025.103851

Seema Devi, Yashoda Nandan Tripathi, Richa Raghuwanshi

Plants as a resource of novel elicitors capable of inhibiting pathogen and promoting crop growth are gaining significant attention. Himalayan plants (Colebrookea oppositifolia, Digitalis purpurea and Rumex hastatus), well documented for their pharmacological value and antimicrobial properties were studied for their growth promoting potentials on wheat. Methanolic crude extract obtained from leaf samples of the selected Himalayan plants were applied on wheat through seed priming and foliar spray method. Morphological parameters selected for phyto-stimulatory assessment of treatments were germination percent, shoot length, root length, seedling fresh weight and dry weight, vigour index, stem thickness and root volume, where significant improvements were recorded. Biochemical changes in response to treatment measured were photosynthetic pigments, total phenolic and flavonoid content, osmolytes and antioxidant activity. Wheat plants showed an increment in all the parameters after treatment with botanicals. The growth-promoting potentials of the selected botanicals were further evaluated for quantifying endogenous phytohormone (IAA and GA) levels in wheat through High-Performance Liquid Chromatography (HPLC). Increased expression of hormones was recorded in wheat, which was treatment specific. Wheat stems examined through transverse sections showed increased lignification around the vascular tissue and hypodermis. This study represents the first documented investigation into the growth-promoting effects of selected botanicals on wheat, providing novel insights into their potential as biostimulants.

植物作为一种能够抑制病原菌和促进作物生长的新型激发子资源正受到越来越多的关注。对喜马拉雅植物（colebrokea oppositifolia， Digitalis purpurea和Rumex hastatus）的药理价值和抗菌特性进行了研究，研究了它们对小麦的促生长潜力。选用喜马拉雅植物叶片样品提取的甲醇粗提物，通过种子灌种和叶面喷雾的方法施用于小麦上。用于植物刺激评价的形态学参数为发芽率、茎长、根长、幼苗鲜重和干重、活力指数、茎粗和根体积，其中有显著改善。光合色素、总酚和类黄酮含量、渗透物和抗氧化活性等生化指标对处理的影响。小麦植株经植物药处理后，各项指标均有增加。利用高效液相色谱法（HPLC）测定小麦内源植物激素（IAA和GA）水平，进一步评价所选植物制剂的促生长潜力。小麦中记录到激素表达增加，这是处理特异性的。小麦茎横切面显示维管组织和皮下组织周围木质化增加。本研究首次对选定植物药对小麦的促生长作用进行了文献调查，为其作为生物刺激素的潜力提供了新的见解。

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引用次数: 0