Biocatalysis and agricultural biotechnology最新文献_第9页

UV/EMS mutagenized thermo-alkalophilic polygalacturonase from Glutamicibacter sp.: Physicochemical insights and biotechnological prospects

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103465

Mary Arpana, Aneesa Fasim, Sunil S. More

The current study discusses the physicochemical properties of an alkalophilic polygalacturonase (PGmut) that was extracted from the UV/EMS mutagenized Glutamicibacter sp. MAIDO R22b-13 strain in comparison to its wildtype (PGwt). Further, its applications in various biotechnology sectors were also assessed. The PGwt and PGmut enzymes were purified by gel permeation chromatography and biochemical analysis determined them to be alkalophilic with pH optima of 10, however mutagenesis enhanced the temperature optima from 37 °C (PGwt) to 50 °C. The molecular weight of both enzymes was confirmed to be 48.67 kDa by SDS-PAGE and LC-MS. PGmut showed higher substrate affinity and velocity, with Km and Vmax of 0.051 mg/ml and 300.12 μmol/min, respectively, compared to 0.072 mg/ml and 205.30 μmol/min for PGwt. Co²⁺ and Mn²⁺ ions enhanced PGmut activity, while Ag²⁺, Cu²⁺, Hg²⁺, and EDTA lowered it. Nevertheless, dialysis-based EDTA removal moderately restored enzyme activity, confirming it to be a metalloenzyme. In addition, PGmut enhanced olive oil production, enabled lycopene extraction from tomato peels, and effectively softened sugarcane bagasse pulp for papermaking. Therefore, this study highlights PGmut's enhanced properties that offers cost-efficient, sustainable, time-saving bioprocess solution, benefiting industry and the environment.

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引用次数: 0

Heterologous laccase from the marine environment: Purification, characterization, and degradation of synthetic dyes

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103485

Igor Vinicius Ramos Otero , Magdalena Haslbeck , Lara Cavalari Santello , Henrique Ferreira , Volker Sieber , Lara Durães Sette

The marine-derived fungus Peniophora sp. CBMAI 1063 is a hyper-producer of laccase. Laccases are multicopper oxidases able to oxidize different aromatic compounds while reducing molecular oxygen to water. Several laccases from terrestrial environments have been purified and characterized. However, little is known about marine-derived laccases. In this study, Pnh_Lac1 (Lac1) from the fungus Peniophora sp. CBMAI 1063 was heterologously expressed in Pichia pastoris, purified, characterized, and used for the degradation/detoxification of synthetic dyes. Lac1 (∼72 kDa) exhibited optimal activity at 60 °C and pH 3, with good thermostability (T₅₀^1h = 56 °C) and high tolerance to metal ions and organic solvents. Lac 1 decolorized/degraded different classes of dyes, under low enzyme concentrations (0.2–0.02 U mL^-1), with an excellent performance regarding the decolorization of Indigo Carmine (93% after 2 h) in the presence of syringaldehyde. Additionally, 65% of the azo dye Reactive Black 5 was degraded by the Lac1-mediator system into lower molecular weight metabolites, with a significant reduction in phytotoxicity. These results demonstrate that the marine-derived Lac1 is a fungal laccase highly active under low concentration, with the potential to mitigate environmental pollutants on biodegradation strategies based on biocatalysis.

{"title":"Heterologous laccase from the marine environment: Purification, characterization, and degradation of synthetic dyes","authors":"Igor Vinicius Ramos Otero , Magdalena Haslbeck , Lara Cavalari Santello , Henrique Ferreira , Volker Sieber , Lara Durães Sette","doi":"10.1016/j.bcab.2024.103485","DOIUrl":"10.1016/j.bcab.2024.103485","url":null,"abstract":"<div><div>The marine-derived fungus <em>Peniophora</em> sp. CBMAI 1063 is a hyper-producer of laccase. Laccases are multicopper oxidases able to oxidize different aromatic compounds while reducing molecular oxygen to water. Several laccases from terrestrial environments have been purified and characterized. However, little is known about marine-derived laccases. In this study, Pnh_Lac1 (Lac1) from the fungus <em>Peniophora</em> sp. CBMAI 1063 was heterologously expressed in <em>Pichia pastoris</em>, purified, characterized, and used for the degradation/detoxification of synthetic dyes. Lac1 (∼72 kDa) exhibited optimal activity at 60 °C and pH 3, with good thermostability (T<sub>50</sub><sup>1h</sup> = 56 °C) and high tolerance to metal ions and organic solvents. Lac 1 decolorized/degraded different classes of dyes, under low enzyme concentrations (0.2–0.02 U mL<sup>-1</sup>), with an excellent performance regarding the decolorization of Indigo Carmine (93% after 2 h) in the presence of syringaldehyde. Additionally, 65% of the azo dye Reactive Black 5 was degraded by the Lac1-mediator system into lower molecular weight metabolites, with a significant reduction in phytotoxicity. These results demonstrate that the marine-derived Lac1 is a fungal laccase highly active under low concentration, with the potential to mitigate environmental pollutants on biodegradation strategies based on biocatalysis.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103485"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in conversion technologies for biofuels from wheat and corn straws 小麦和玉米秸秆生物燃料转化技术的进展

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103481

Elisiane Rocha Lufan Prado, Rafael Cardoso Rial

This work reviews recent technologies and processes for the production of biofuels from wheat and corn straw, highlighting their potential as renewable energy sources. These often underutilized agricultural residues offer a significant opportunity for the production of bioethanol, biogas, and bio-oil, contributing to the diversification of the energy matrix and the mitigation of greenhouse gas emissions. The analysis details the impact of the chemical constituents of these straws on the efficiency of conversion processes, such as lignin and phenolic acids, which can inhibit the production of bioethanol and biogas. Among the technological advances, methods such as microwave-assisted pyrolysis and the use of ionic liquids have shown significant improvements in conversion efficiency, removing inhibitors and increasing biofuel productivity. However, these processes still face challenges related to scalability and costs. Additionally, the use of wheat and corn straw can add economic value for farmers and promote rural development, creating new sources of income and encouraging a circular economy. Overall, this study concludes that second-generation biofuels derived from wheat and corn straw hold immense potential to address environmental and economic challenges, but their large-scale adoption requires continued technological innovation and supportive public policies.

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引用次数: 0

Potential use of Ecdysone in protecting wheat (Triticum aestivum) germination under cadmium stress

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103479

Mostafa Lamhamdi , Saliha Gharbi , Mohamed El Arras , Ouiam El Galiou , Hamza El Moudden , Learn-Han Lee , Abdelhakim Bouyahya , Ahmed Bakrim

The purpose of this work is to explore the potential of Ecdysone (Ecd) in protecting wheat seedlings from the toxic effects of cadmium (Cd). Various growth parameters such as germination, tolerance index, plant biomass, and vigor index were assessed. Additionally, germination metabolic parameters including soluble sugar content, soluble proteins, free amino acids, amylase, and protease activities were measured. During this study, we investigated the stress markers content, proline, chlorophyll, and antioxidant enzymes. The amount of Cd uptake and its distribution at the cellular level were evaluated. In the absence of pretreatment. The results showed that high concentrations of Cd (0.1 and 0.5 mM) caused a decrease in germination (13%–35%) and growth (24%–53%). In addition, indicators of oxidative stress were observed, suggesting an alteration of physiological processes. Key germination enzymes α-amylase and proteases activities decreased with increasing Cd concentration, leading to a reduction in solubles sugar (48%–64%) and amino acid (55%–64%) levels. Ecdysone pretreatment (10 μM) significantly mitigated Cd toxicity in young plants by decreasing its absorption (71%–40% in leaf and root respectively at 0.5 mM Cd), alleviating oxidative stress (36%–43% reducing MDA), and enhancing detoxification processes. In addition, Ecd pretreatment maintained normal germination, tolerance, biomass, and metabolism in the presence of Cd. According to our results, Ecdysone ensures the preservation of oxidative balance and promotes wheat germination metabolism.

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引用次数: 0

Mitigation of H2O2 induced Reactive Oxygen species and Proinflammatory cytokines by Buckwheat (Fagopyrum tataricum) rutin in cultured RAW264.7 cells

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103462

Dennis Soosai , R. Ravindhran , Elumalai Perumal , C. Sheela Sasikumar , Charles P , Leo Arockia Raj S

Background

Buckwheat rutin, sourced from the Himalayan region of Nepal, exhibits significant free radical-scavenging potential. The rutin fraction derived from a tartary buckwheat seeds cultivar NGRC03731 exerts protective effects by preventing cellular damage and regulating proinflammatory cytokines in the mouse macrophage cell line RAW264.7.

Methods

The rutin fraction antioxidant properties were investigated using DPPH and ABTS methods in a cell-free system. RAW 264.7 cell viability following exposure to rutin fraction and standard rutin in the presence or absence of H₂O₂ was determined using the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay. Free radical generation was evaluated through DCFDA staining. Pro-inflammatory cytokine gene expression levels were assessed using real-time PCR, while the corresponding protein levels were measured using Enzyme linked immunosorbent assay (ELISA).

Results

The rutin fraction markedly reduced NO and PGE2 levels. The viability of RAW 264.7 cells exposed to H₂O₂ and co-treated with the rutin fraction retained cell viability. Moreover, the rutin fraction alone (200 μg/mL) did not alter the morphology of RAW 264.7 cells, and reduced cellular damage in the presence of H₂O₂. TNF-α, COX-2a, IL-1, IL-6, IL-8, and IL-10 gene expression was significantly downregulated as well the cytokine protein levels of TNF-α, IL-1β, IL-8, IL-6, and IL-10 were markedly down regulated in RAW 264.7 cells treated with the rutin fraction.

Conclusion

The results of our study demonstrate the remarkable anti-inflammatory properties of the buckwheat rutin fraction in suppressing inflammatory responses.

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引用次数: 0

Optimizing extraction techniques for enhanced polyphenol yield from Rheum emodi and Urtica dioica: Comparative analysis of solvent systems and methods

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103475

Saadiya Naqash, Tajamul Rouf Shah, Arooj Manzoor Matoo, Darakshan Majid, Tehmeena Ahad, Sajad A. Sofi, Khalid Muzaffar, Hilal A. Makroo, B.N. Dar

The study optimized polyphenol extraction from Rheum emodi and Urtica dioica, varying solvents (Ethanol, Methanol, Water), concentrations, and extraction methods (CE, MAE, UAE). UAE consistently outperformed, yielding significantly higher phenolics, flavonoids, and antioxidants. In the 60% methanolic extract of U. diocia, the highest antioxidant activities were recorded, with DPPH (69.4 ± 2.2%), ABTS (94.61 ± 2.1%), and FRAP (0.82 ± 0.005 mol Fe2+/g). For Rheum emodi, the 70% ethanolic extract exhibited superior flavonoid content (63.6 ± 2.1 mg RE/g), phenolic content (132.7 ± 2.2 mg E GA/g), and antioxidant activities, including DPPH (69.4 ± 2.2%), ABTS (94.6 ± 2.1%), and FRAP (0.82 ± 0.005 μmol Fe2+/g). HPLC analysis identified eight phenolic acids, five flavonoids, and two anthraquinones in both extracts. These results highlight the potential of Rheum emodi and Urtica dioica as rich sources of phytochemicals for various food and pharmaceutical applications.

该研究通过改变溶剂（乙醇、甲醇、水）、浓度和萃取方法（CE、MAE、UAE），对大黄和荨麻中的多酚萃取进行了优化。UAE 的表现始终优于其他方法，酚类、类黄酮和抗氧化剂的产量明显更高。在 U. diocia 的 60% 甲醇提取物中，DPPH（69.4 ± 2.2%）、ABTS（94.61 ± 2.1%）和 FRAP（0.82 ± 0.005 mol Fe2+/g）的抗氧化活性最高。大黄的 70% 乙醇提取物显示出较高的类黄酮含量（63.6 ± 2.1 mg RE/g）、酚含量（132.7 ± 2.2 mg E GA/g）和抗氧化活性，包括 DPPH（69.4 ± 2.2%）、ABTS（94.6 ± 2.1%）和 FRAP（0.82 ± 0.005 μmol Fe2+/g）。高效液相色谱分析确定了两种提取物中的八种酚酸、五种黄酮类化合物和两种蒽醌类化合物。这些结果凸显了大黄和荨麻作为植物化学物质的丰富来源在各种食品和医药应用中的潜力。

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引用次数: 0

Comparative phenolic profile and antioxidant potential of mentha hairy roots and aerial parts

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103469

Fazileh Esmaeili, Zahra Rahimi, Shirin Yousefian, Mohsen Farhadpour, Tahmineh Lohrasebi

The Mentha genus is a well-known medicinal plant and is found to contain various phenolic compounds. Most of these compounds are typically extracted; however, utilizing biotechnology methods, such as hairy root culture, offers a viable option for producing these compounds. Hairy roots were generated from four Mentha species, including Mentha spicata, Mentha piperita, Mentha aquatica, and Mentha longifolia. Afterward, the phenolic content and antioxidant properties of both parts were compared. The results indicated that both the type of explant and the species studied are crucial factors in determining the types and levels of phenolic compounds present. It was also found that the highest total phenol and flavonoid contents were related to the aerial parts extracts of M. aquatica and M. spicata, while these contents were higher in the hairy roots of M. piperita and M. longifolia. Moreover, the HPLC analysis of the aerial parts extracts of different Mentha species showed that the highest levels of caffeic acid, chlorogenic acid, rosmarinic acid, and quercetin were found in M. spicata. However, in the case of hairy roots, M. piperita extract showed the highest amounts of these compounds. In general, the amount of secondary metabolites in the hairy root extract was lower than in the aerial parts. Additionally, the highest level of antioxidant properties was found in the leaf extracts. In conclusion, the aerial parts of the Mentha species have more phenolic compounds than the hairy roots under conditions where no inducers or growth stimulants are used.

{"title":"Comparative phenolic profile and antioxidant potential of mentha hairy roots and aerial parts","authors":"Fazileh Esmaeili, Zahra Rahimi, Shirin Yousefian, Mohsen Farhadpour, Tahmineh Lohrasebi","doi":"10.1016/j.bcab.2024.103469","DOIUrl":"10.1016/j.bcab.2024.103469","url":null,"abstract":"<div><div>The <em>Mentha</em> genus is a well-known medicinal plant and is found to contain various phenolic compounds. Most of these compounds are typically extracted; however, utilizing biotechnology methods, such as hairy root culture, offers a viable option for producing these compounds. Hairy roots were generated from four <em>Mentha</em> species, including <em>Mentha spicata</em>, <em>Mentha piperita</em>, <em>Mentha aquatica</em>, and <em>Mentha longifolia</em>. Afterward, the phenolic content and antioxidant properties of both parts were compared. The results indicated that both the type of explant and the species studied are crucial factors in determining the types and levels of phenolic compounds present. It was also found that the highest total phenol and flavonoid contents were related to the aerial parts extracts of <em>M. aquatica</em> and <em>M. spicata</em>, while these contents were higher in the hairy roots of <em>M. piperita</em> and <em>M. longifolia</em>. Moreover, the HPLC analysis of the aerial parts extracts of different <em>Mentha</em> species showed that the highest levels of caffeic acid, chlorogenic acid, rosmarinic acid, and quercetin were found in <em>M. spicata</em>. However, in the case of hairy roots, <em>M. piperita</em> extract showed the highest amounts of these compounds. In general, the amount of secondary metabolites in the hairy root extract was lower than in the aerial parts. Additionally, the highest level of antioxidant properties was found in the leaf extracts. In conclusion, the aerial parts of the <em>Mentha</em> species have more phenolic compounds than the hairy roots under conditions where no inducers or growth stimulants are used.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103469"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of nucleotide diversity among alleles of intronless bacterial blight resistant genes Xa27 and Xa23 in rice

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103477

Prajna Priyadarshini Das , Kailash Pati Bhandari , Komal Bhati , C. Gireesh , Gouri Sankar Laha , Raman Meenakshi Sundaram , Irfan Ahmad Ghazi

Identifying novel effective alleles from different gene pools can provide a stress-free response in any crop plant by allele mining approach. Nucleotide diversity analysis through allele mining helps to study diverse function of genes and their role in disease resistance enhancement. The executor R genes; Xa27 and Xa23 provide resistance against several strains of the rice bacterial blight pathogen, Xanthomonas oryzae pv. Oryzae (Xoo). A total 114 wild relatives of Oryza species were examined to assess nucleotide diversities among the natural alleles of the two genes. Among these, 21 accessions were identified as homologous to Xa27, and 5 accessions were homologous to Xa23. Pathogenicity test was conducted to assess the resistance efficiency of the gathered wild accessions using disease scoring assays for phenotyping. Various parameters for the nucleotide diversity study, including polymorphic sites, InDels, number of haplotypes, single nucleotide polymorphisms, as well as Tajima's D and Fu and Li's D tests, were analyzed for the identified alleles in both the promoter, Effector Binding Element (EBE), and coding regions of these two genes. Xa27 alleles from the rice accessions such as CG86, CG105, CG180, and CG02 exhibit higher numbers of substitutions compared to other identified alleles. CG12 accession showed numerous nucleotide substitutions, indicating a possible novel allele unrelated to Xa23 alleles. Molecular mining of Xa27 and Xa23 in wild rice relatives will help to identify the major novel allele for the breeding program to enhance crop improvement.

{"title":"Analysis of nucleotide diversity among alleles of intronless bacterial blight resistant genes Xa27 and Xa23 in rice","authors":"Prajna Priyadarshini Das , Kailash Pati Bhandari , Komal Bhati , C. Gireesh , Gouri Sankar Laha , Raman Meenakshi Sundaram , Irfan Ahmad Ghazi","doi":"10.1016/j.bcab.2024.103477","DOIUrl":"10.1016/j.bcab.2024.103477","url":null,"abstract":"<div><div>Identifying novel effective alleles from different gene pools can provide a stress-free response in any crop plant by allele mining approach. Nucleotide diversity analysis through allele mining helps to study diverse function of genes and their role in disease resistance enhancement. The executor R genes; <em>Xa27</em> and <em>Xa23</em> provide resistance against several strains of the rice bacterial blight pathogen, <em>Xanthomonas oryzae</em> pv. <em>Oryzae</em> (<em>Xoo</em>). A total 114 wild relatives of <em>Oryza</em> species were examined to assess nucleotide diversities among the natural alleles of the two genes. Among these, 21 accessions were identified as homologous to <em>Xa27</em>, and 5 accessions were homologous to <em>Xa23</em>. Pathogenicity test was conducted to assess the resistance efficiency of the gathered wild accessions using disease scoring assays for phenotyping. Various parameters for the nucleotide diversity study, including polymorphic sites, InDels, number of haplotypes, single nucleotide polymorphisms, as well as Tajima's D and Fu and Li's D tests, were analyzed for the identified alleles in both the promoter, Effector Binding Element (EBE), and coding regions of these two genes. <em>Xa27</em> alleles from the rice accessions such as CG86, CG105, CG180, and CG02 exhibit higher numbers of substitutions compared to other identified alleles. CG12 accession showed numerous nucleotide substitutions, indicating a possible novel allele unrelated to <em>Xa23</em> alleles. Molecular mining of <em>Xa27</em> and <em>Xa23</em> in wild rice relatives will help to identify the major novel allele for the breeding program to enhance crop improvement.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103477"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of palm petiole waste as natural biocoagulants: Optimizing coagulation-flocculation for sustainable wastewater treatment and advancing circular economy in agriculture

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103473

Ali Zourif, Salma Kouniba, Mohamed El Guendouzi

This study investigates the potential of palm petiole waste (PPW) and its extracted lignin as natural biocoagulants for removing methylene blue, crystal violet dyes, and turbidity from wastewater. Comprehensive characterization using X-ray diffraction, fourier-transform infrared spectroscopy, scanning electron microscopy coupling with energy-dispersive X-ray spectroscopy, X-ray fluorescence, and elemental analysis revealed the distinct physicochemical properties of PPW and lignin. A Box-Behnken experimental design optimized coagulation conditions, achieving 98.26 % pollutant removal with PPW and 90.17 % with lignin. Crucially, PPW's superior performance stems from a synergy of adsorption, charge neutralization by metal salts, and bridging effects, while lignin relies primarily on adsorption and bridging. This challenges the notion that lignin alone dictates coagulation efficacy in lignocellulosic wastes. The study also explored the economic and environmental implications of using PPW as a bio coagulants-flocculant, demonstrating significant reductions in carbon footprint, energy consumption, and water use compared to conventional chemical coagulants.

{"title":"Valorization of palm petiole waste as natural biocoagulants: Optimizing coagulation-flocculation for sustainable wastewater treatment and advancing circular economy in agriculture","authors":"Ali Zourif, Salma Kouniba, Mohamed El Guendouzi","doi":"10.1016/j.bcab.2024.103473","DOIUrl":"10.1016/j.bcab.2024.103473","url":null,"abstract":"<div><div>This study investigates the potential of <em>palm petiole waste</em> (PPW) and its extracted lignin as natural biocoagulants for removing methylene blue, crystal violet dyes, and turbidity from wastewater. Comprehensive characterization using X-ray diffraction, fourier-transform infrared spectroscopy, scanning electron microscopy coupling with energy-dispersive X-ray spectroscopy, X-ray fluorescence, and elemental analysis revealed the distinct physicochemical properties of PPW and lignin. A Box-Behnken experimental design optimized coagulation conditions, achieving 98.26 % pollutant removal with PPW and 90.17 % with lignin. Crucially, PPW's superior performance stems from a synergy of adsorption, charge neutralization by metal salts, and bridging effects, while lignin relies primarily on adsorption and bridging. This challenges the notion that lignin alone dictates coagulation efficacy in lignocellulosic wastes. The study also explored the economic and environmental implications of using PPW as a bio coagulants-flocculant, demonstrating significant reductions in carbon footprint, energy consumption, and water use compared to conventional chemical coagulants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103473"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capsicum chinense cell cultures: A biotechnological platform for the sustainable production of bioactive metabolites for the cosmetics market

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.bcab.2024.103478

Amir Akhgari , Maria Sulli , Anna Ramata-Stunda , Suvi T. Häkkinen , Liisa Nohynek , Aleksander Salwinski , Olivia Costantina Demurtas , Mārtiņš Borodušķis , Maria Pajumo , Richard M. Twyman , Kirsi-Marja Oksman-Caldentey , Gianfranco Diretto , Heiko Rischer

Capsicum species produce a broad range of bioactive metabolites with properties relevant to the cosmetics industry. However, the direct sourcing from Capsicum plants is hindered by the low and variable yields, which depend on genotype and environmental conditions, and by the pungent components of chili plants that act as irritants. We therefore set out to derive callus and cell suspension cultures from the non-pungent variety C. chinense cv. Trinidad Pimento as a sustainable and consistent source. Cultures were grown at the 2-L scale in darkness (D) or with a photoperiodic light source (P) and we prepared hydroethanolic and ethyl acetate extracts for the analysis of metabolic profiles and activities. Comprehensive untargeted metabolomic analysis revealed different clusters of metabolites under D and P conditions, although phenolamides and fatty acids were the most abundant compounds in both cases. Targeted analysis indicated the presence of capsiate, dihydrocapsiate and nordihydrocapsiate as key bioactive products in the hydroethanolic extracts and 18 different carotenoids in the ethyl acetate extracts. Bioactivity assays applied to the hydroethanolic extracts indicated significant antioxidant activity and the ability to inhibit the growth of methicillin resistant Staphylococcus aureus. The extracts were neither cytotoxic nor phototoxic toward BALB/c 3T3 and HaCaT cell lines at concentrations low enough to exert antimicrobial effects. Our work shows that C. chinense cv. Trinidad Pimento cell cultures can produce specific metabolites in response to different cultivation conditions, which will allow their exploitation as a sustainable source of safe and efficacious ingredients for the cosmetics industry.

{"title":"Capsicum chinense cell cultures: A biotechnological platform for the sustainable production of bioactive metabolites for the cosmetics market","authors":"Amir Akhgari , Maria Sulli , Anna Ramata-Stunda , Suvi T. Häkkinen , Liisa Nohynek , Aleksander Salwinski , Olivia Costantina Demurtas , Mārtiņš Borodušķis , Maria Pajumo , Richard M. Twyman , Kirsi-Marja Oksman-Caldentey , Gianfranco Diretto , Heiko Rischer","doi":"10.1016/j.bcab.2024.103478","DOIUrl":"10.1016/j.bcab.2024.103478","url":null,"abstract":"<div><div>Capsicum species produce a broad range of bioactive metabolites with properties relevant to the cosmetics industry. However, the direct sourcing from Capsicum plants is hindered by the low and variable yields, which depend on genotype and environmental conditions, and by the pungent components of chili plants that act as irritants. We therefore set out to derive callus and cell suspension cultures from the non-pungent variety <em>C. chinense</em> cv. Trinidad Pimento as a sustainable and consistent source. Cultures were grown at the 2-L scale in darkness (D) or with a photoperiodic light source (P) and we prepared hydroethanolic and ethyl acetate extracts for the analysis of metabolic profiles and activities. Comprehensive untargeted metabolomic analysis revealed different clusters of metabolites under D and P conditions, although phenolamides and fatty acids were the most abundant compounds in both cases. Targeted analysis indicated the presence of capsiate, dihydrocapsiate and nordihydrocapsiate as key bioactive products in the hydroethanolic extracts and 18 different carotenoids in the ethyl acetate extracts. Bioactivity assays applied to the hydroethanolic extracts indicated significant antioxidant activity and the ability to inhibit the growth of methicillin resistant Staphylococcus aureus. The extracts were neither cytotoxic nor phototoxic toward BALB/c 3T3 and HaCaT cell lines at concentrations low enough to exert antimicrobial effects. Our work shows that <em>C. chinense</em> cv. Trinidad Pimento cell cultures can produce specific metabolites in response to different cultivation conditions, which will allow their exploitation as a sustainable source of safe and efficacious ingredients for the cosmetics industry.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103478"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0