Biocatalysis and agricultural biotechnology最新文献_第2页

The cytotoxic potential of water-methanol-extracted secondary metabolites from lichens as an important source for treating human cancer cell lines 地衣水甲醇萃取次生代谢物作为治疗人类癌细胞的重要来源，具有潜在的细胞毒性

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-13 DOI: 10.1016/j.bcab.2026.103934

Łukasz Furmanek , Natalia Żurek , Ireneusz Kapusta , Mark R.D. Seaward , Paweł Czarnota

The results of in vitro experiments on the cytotoxic potential of water-methanol extracts obtained from 11 lichen species, Cetraria islandica, Cladonia arbuscula, Cladonia digitata, Cladonia gracilis, Cladonia phyllophora, Cladonia rangiferina, Cladonia uncialis, Hypogymnia physodes, Platismatia glauca, Pseudevernia furfuracea and Usnea dasopoga, against five human cancer cell lines - AGS, Dld-1, U251mg, LS180 and Ht-29 are presented. The antioxidant potential of the extracts was determined by means of the DPPH˙, FRAP and chelating ability of metals ion (ChP) tests. The highest activity for DPPH˙ (225.33 mmol TE/100 g d.m.) and FRAP (229.02 mmol TE/100 g d.m.) tests were shown for C. digitata extract, while for the ChP test, the extracts obtained from C. digitata and H. physodes species (IC₅₀: 2.10 mg/mL). For the cytotoxicity test, the strongest potential was shown for the C. digitata extract against all the tested cancer cell lines - AGS (IC₅₀: 123.14 μg/mL), Dld-1 (113.20 μg/mL), U251mg (137.20 μg/mL), LS180 (125.67 μg/mL) and Ht-29 (111.64 μg/mL). Slightly weaker cytotoxic efficacy was shown by extracts obtained from H. physodes and P. furfuracea. The qualitative-quantitative analysis of the profile of extracted lichen secondary metabolites performed by UPLC-Q-TOF-MS/MS identified 30 secondary compounds, the most abundant of which were fumarprotocetraric acid, physodic acid isomer, 3-hydroxyphysodic acid and usnic acid. The cytotoxicity potential of the tested extracts against AGS, DLD-1 and LS180 cell lines has been performed as a first approach worldwide. The results of all tests and analyses showed research opportunities in phytotherapy in the future.

本文报道了海岛天蚕、灌木天蚕、digitata天蚕、花叶天蚕、rangiferina天蚕、uncladonia uncalalis、Hypogymnia physodes、Platismatia glauca、furfuracea pseudovernia和Usnea dasopoga等11种地衣水甲醇提取物对5种人类癌细胞AGS、ddd -1、U251mg、LS180和Ht-29的体外毒性实验结果。通过DPPH˙、FRAP和金属离子螯合能力（ChP）测试来测定提取物的抗氧化能力。在DPPH˙（225.33 mmol TE/100 g d.m.）和FRAP （229.02 mmol TE/100 g d.m.）试验中，金针桃提取物的活性最高，而在ChP试验中，金针桃和金针桃提取物的IC50为2.10 mg/mL。在细胞毒性实验中，对AGS （IC50: 123.14 μg/mL）、Dld-1 （113.20 μg/mL）、U251mg （137.20 μg/mL）、LS180 （125.67 μg/mL）和tt -29 （111.64 μg/mL）的抑制作用最强。植酸草和糠醛草提取物的细胞毒作用稍弱。利用ulc -q - tof -MS/MS对提取的地衣次生代谢物进行定性定量分析，鉴定出30种次生化合物，其中富马原二酸、植酸异构体、3-羟基植酸和枸杞酸含量最高。所测试的提取物对AGS， DLD-1和LS180细胞系的细胞毒性潜力已在全球范围内首次进行。所有测试和分析的结果表明，植物疗法在未来的研究机会。

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引用次数: 0

The promising effects of bacterial and microalgal inoculants in enhancing tomato growth and improving soil quality 细菌和微藻接种剂在促进番茄生长和改善土壤质量方面的前景

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-13 DOI: 10.1016/j.bcab.2026.103937

Tongtong Xu , Qixiang Xian , Yunqian Cui , Huali Pei , Wei Liu , Mingjun Chen , Jingling Liu , Hongbo Yu , Nortoji A. Khujamshukurov , Meimei Wu

The overuse of chemical fertilizers threatens soil health and sustainable agriculture. While bacterial biofertilizers are widely used, microalgae offer a promising alternative due to their low cost and accessibility. This study investigated the effects of bacterial and microalgal inoculants on tomato (Solanum lycopersicum L.) growth, fruit quality, and soil properties through a 100-day pot experiment. The results showed that the application of composite bacteria inoculant and microalgae inoculant significantly promoted tomato growth, increased soil nutrient levels, and improved tomato yield and fruit quality. At 100 days post-inoculation, the T4 group (60 mL combined bacterial inoculant) exhibited the most pronounced growth-promoting effects on tomato plants. Compared with the T1 group (soil not inoculated with bacteria/microalgae), the T4 group significantly enhanced plant height (13.8 %), leaf number (48.3 %), leaf dimensions (19.7 % and 31.2 % in length and width, respectively), and stem diameter (11.3 %). Regarding fruit quality, the T4 group yielded tomatoes with the highest vitamin C content (23.52 ± 0.30 mg·100 g⁻¹). Notably, among all microalgae treatments, the T8 group (Tribonema sp.) demonstrated optimal performance, showing significantly higher contents of both fructose (3.62 ± 0.13 mg g⁻¹) and titratable acid (0.29 ± 0.03 %) in fruits compared to other groups. Actinobacteriota and Proteobacteria were the dominant phyla in all soil groups. This study contributes to the systematic evaluation of the promoting effects and application prospects of composite bacteria inoculant and microalgae inoculant for enhancing crop growth, yield, and soil amelioration.

化肥的过度使用威胁着土壤健康和农业的可持续发展。虽然细菌生物肥料被广泛使用，但微藻由于其低成本和可获得性而提供了一个有前途的替代品。通过100天盆栽试验，研究了细菌和微藻接种剂对番茄生长、果实品质和土壤性状的影响。结果表明，施用细菌和微藻复合接种剂可显著促进番茄生长，提高土壤养分水平，提高番茄产量和果实品质。接种100 d后，T4组（60ml联合细菌接种剂）对番茄植株的促生长作用最为显著。与T1组（未接种细菌/微藻的土壤）相比，T4组的株高（13.8%）、叶片数（48.3%）、叶片尺寸（长、宽分别为19.7%和31.2%）和茎粗（11.3%）显著提高。在果实品质方面，T4组番茄的维生素C含量最高（23.52±0.30 mg·100 g−1）。值得注意的是，在所有微藻处理中，T8组（Tribonema sp.）表现最佳，果实中果糖（3.62±0.13 mg g−1）和可滴定酸（0.29±0.03%）含量均显著高于其他处理组。放线菌门和变形菌门是各土壤类群的优势门。本研究系统评价了细菌和微藻复合接种剂对作物生长、产量和土壤改良的促进作用和应用前景。

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引用次数: 0

Three-phase partitioning (TPP) purification, characterization and dye decolorization application of laccase from Pseudolagarobasidium acaciicola TDW-48 acaciicola Pseudolagarobasidium TDW-48漆酶的三相分割纯化、表征及染料脱色应用

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-13 DOI: 10.1016/j.bcab.2026.103940

Thi Thu Huong Luong, Supattra Poeaim

This study focused on the efficient and economical purification of laccase from P. acaciicola TDW-48 and its application in dye decolorization. Firstly, the P. acaciicola TDW-48's laccase was purified by the three-phase partitioning (TPP) method, and then the artificial neural network-genetic algorithm (ANN-GA) optimization was used to improve the purification yield. The result indicated that the TPP method successfully purified P. acaciicola TDW-48's laccase with a high purification yield. After ANN-GA optimization, the strong interactive effects of the TPP parameters on purification yield were demonstrated by a high-accuracy ANN model (R-value of 0.99918 for all datasets). An optimum TPP system was developed, achieving 138.7 % activity recovery and 1.62-fold purity at a 57.82 % salt concentration, pH 5.75, and a t-butanol/enzyme ratio of 1.5. In the following, the enzyme characteristics and application potential of purified laccase were determined. The P. acaciicola TDW-48's laccase showed a molecular weight of 60.5 kDa and functioned optimally at pH 3 and 30 °C. The kinetic parameters implied high affinity and catalytic efficiency for the ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) substrate, with a low Km (37.9 μM) and a high Vmax (46.01 mM/min). Moreover, the purified laccase showed excellent potential for dye decolorization, with 44 % Congo red, 80 % bromophenol blue, and 58 % phenol red decolorized after 8 h of treatment.

本文主要研究了从紫杉树TDW-48中高效、经济地提纯漆酶及其在染料脱色中的应用。首先，采用三相分割（TPP）法对紫杉树TDW-48漆酶进行纯化，然后采用人工神经网络遗传算法（ANN-GA）优化提高纯化收率。结果表明，采用TPP法纯化了紫杉树TDW-48的漆酶，纯化率较高。经过ANN- ga优化后，通过高精度的ANN模型（所有数据集的r值为0.99918）证明了TPP参数对纯化率的强交互效应。在盐浓度为57.82%、pH为5.75、t-丁醇/酶比为1.5的条件下，最佳TPP体系的活性回收率为138.7%，纯度为1.62倍。本文对纯化漆酶的酶学特性和应用潜力进行了探讨。该漆酶的分子量为60.5 kDa，在pH为3、温度为30℃的条件下具有最佳的酶活性。动力学参数表明，ABTS（2,2′-氮基-双（3-乙基苯并噻唑-6-磺酸））底物具有较低的Km （37.9 μM）和较高的Vmax (46.01 mM/min)，具有较高的亲和力和催化效率。此外，纯化的漆酶表现出良好的染料脱色潜力，经过8小时的处理，脱色率为44%刚果红，80%溴酚蓝和58%酚红。

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引用次数: 0

Consolidated bio-saccharification (CBS) enhanced hydrogen production via bioaugmentation to eliminate cellobiose inhibition: Investigation on hydrogen production performance and microbial community 强化生物糖化（CBS）通过生物强化来消除纤维二糖抑制来提高制氢：产氢性能和微生物群落的研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-13 DOI: 10.1016/j.bcab.2026.103938

Sheng Tao , Song Chengwei , Liu Qianchi , Song Zhiwei , Yang Chunxue , Sun Caiyu , Li Lixin , Huang Linlin , Wang Miao

Acetivibrio thermocellus is a key thermophilic, cellulolytic bacterium used in Consolidated bio-saccharification (CBS) for hydrogen production from lignocellulosic biomass. However, the main saccharification product of cellulosic feedstocks, cellobiose, has strong inhibition on A. thermocellus which inhibited cellulase activity and limit overall saccharification and biofuel production efficiency. In this study, a wild-type Acetivibrio thermocellus (strain M3), that could bypass cellobiose inhibition was co-cultured with different hydrogen-producing inocula to test the feasibility of hydrogen production bioaugmentation to eliminate cellobiose inhibition. The results revealed that the addition of A. thermocellus M3 promoted hydrogen production ranging from 42.9 % to 105 %, with the peak hydrogen production obtained from domesticated compost combined with strain M3 (233.02 mL/g). A. thermocellus M3 positively correlated with the hydrogen-producing bacteria. The addition of Acetivibrio thermocellus M3 increased the relative abundance of genes associated with cellulosic feedstocks hydrolysis. Furthermore, the relative abundance of genes associated with stress tolerance and sugar uptake also increased—these genes might help the consolidated bio-saccharification (CBS) hydrogen production system adapt to adverse conditions and thereby alleviate cellobiose inhibition.

热细胞活动弧菌（actiactivibrio thermocellus）是一种关键的嗜热降解纤维素细菌，用于木质纤维素生物质的固结生物糖化（CBS）制氢。然而，纤维素原料的主要糖化产物纤维素二糖对A. thermocellus有很强的抑制作用，抑制了纤维素酶的活性，限制了整体糖化和生物燃料的生产效率。本研究通过与不同产氢疫苗共培养一株能绕过纤维素二糖抑制的野生型热胞活动弧菌（M3菌株），验证产氢生物增菌消除纤维素二糖抑制的可行性。结果表明，添加热cella . M3菌对发酵产氢的促进作用在42.9% ~ 105%之间，其中驯化堆肥与菌株M3组合产氢最高（233.02 mL/g）。A. thermocellus M3与产氢菌呈正相关。热胞活动弧菌M3的添加增加了纤维素原料水解相关基因的相对丰度。此外，与胁迫耐受性和糖摄取相关的基因的相对丰度也有所增加，这些基因可能有助于巩固生物糖化（CBS）制氢系统适应不利条件，从而减轻纤维二糖抑制。

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引用次数: 0

Optimizing humification and valorization of sawdust and manure via bioaugmentation and moisture control in composting 通过生物强化和水分控制优化木屑和粪肥在堆肥中的腐殖质化和增值

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-13 DOI: 10.1016/j.bcab.2026.103928

Nedio Ricardo Rogoski , Sabrina Pinto Salamoni , Gustavo Graciano Fonseca

The objective of this study was to produce and characterize compounds obtained from sawdust and swine and bovine waste, as well as their combinations. Three composting strategies using solid bovine manure (SBM), solid swine manure (SSM), raw wood sawdust (RWS), and decomposed wood sawdust (DWS) were evaluated over a period of 28–60 days to optimize organic waste valorization. In Experiment 1, mixtures containing 10 % RWS achieved rapid moisture equilibration and reached mesophilic temperatures (∼35 °C) within 15 days, whereas 100 % SBM or SSM remained below ambient (25 °C) throughout. In Experiment 2, adjusting initial moisture to 60 % triggered a 24-h thermophilic surge up to 43 °C, and C/N ratios declined from 21.7 to 26.8 to ∼10 by day 28. Microbial counts increased linearly, indicating activation of indigenous microbiota without inoculum. In Experiment 3, bioaugmentation with Bacillus sp. and Penicillium sp. increased total nitrogen from ∼1.2 % to 1.6 % and accelerated C/N ratio reduction to <17:1, while urea addition suppressed microbial growth and limited humic substance formation to 44.5 %. Across all experiments, ash content increased by 15–25 %, and humification (measured as total humic extract) increased by up to 49.5 % in inoculated treatments. It was concluded that (i) RWS improves moisture balance and aeration, (ii) 60 % moisture and bioaugmentation optimize thermophilic activation and organic matter degradation, and (iii) excessive inorganic nitrogen (urea) can inhibit microbial activity and humification.

本研究的目的是生产和表征从木屑和猪和牛的废物中获得的化合物，以及它们的组合。采用固体牛粪（SBM）、固体猪粪（SSM）、原木屑（RWS）和分解木屑（DWS） 3种堆肥策略，在28-60 d的时间内对有机废物的价值最大化进行了评价。在实验1中，含有10% RWS的混合物实现了快速水分平衡，并在15天内达到中温温度（~ 35℃），而100% SBM或SSM始终低于环境温度（25℃）。在实验2中，将初始湿度调整到60%，可触发24小时的嗜热激增，温度高达43°C，到第28天，C/N比率从21.7下降到26.8，再下降到~ 10。微生物数量呈线性增加，表明原生微生物群在没有接种的情况下被激活。在实验3中，芽孢杆菌和青霉菌的生物强化使总氮从~ 1.2%增加到1.6%，C/N加速降低到17:1，而尿素抑制了微生物的生长，将腐殖质形成限制在44.5%。在所有试验中，接种处理的灰分含量增加了15 - 25%，腐殖质（以总腐殖质提取物衡量）增加了49.5%。结果表明：(1)RWS改善了水分平衡和曝气，(2)60%的水分和生物增湿优化了嗜热活化和有机物降解，(3)过量的无机氮（尿素）抑制了微生物活性和腐殖化。

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引用次数: 0

Volatile profile of Calicotome spinosa (L.) Link. revealed by SPME-GC-MS: Phytotoxic and insecticidal activities supported by in silico analysis 花椰菜（calictomome spinosa）的挥发性特征链接。SPME-GC-MS显示：植物毒性和杀虫活性得到硅分析的支持

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-12 DOI: 10.1016/j.bcab.2026.103931

Roukia Zatout , Ouided Benslama , Chaima Zatout , Saliha Kaouache , Stefania Garzoli

In the search for eco-friendly alternatives to synthetic agrochemicals, the volatile organic compounds (VOCs) of Calicotome spinosa (L.) Link, a Mediterranean shrub valued for its medicinal and ecological significance, were investigated. Molecular identification using ITS sequencing showed high similarity to reference material (accession PX285687). The study aimed to characterize the plant's volatiles through solid-phase microextraction coupled with gas chromatography–mass spectrometry (SPME-GC–MS) and to evaluate their phytotoxic and insecticidal properties, supported by in silico molecular docking. SPME-GC–MS analysis identified 20 VOCs, with hexahydrofarnesyl acetone (33.6 %), dihydroactinidiolide (13.4 %), trans-β-ionone (12.7 %), farnesane (6.0 %), and 3,5-di-tert-butylphenol (5.6 %) as major constituents. Bioassays showed that hexane and dichloromethane extracts exhibited strong, dose-dependent phytotoxicity against Lens culinaris, significantly inhibiting germination and seedling growth. Insecticidal assays demonstrated potent fumigant activity: dichloromethane extract caused up to 100 % mortality of Stegobium paniceum and 90 % of Ephestia kuehniella within 72 h, while n-hexane showed slightly lower efficacy. Residual toxicity confirmed consistent mortality across treatments. Molecular docking indicated strong binding of hexahydrofarnesyl acetone and 3,5-di-tert-butylphenol to acetylcholinesterase (AChE) and the octopamine receptor (Oamb) in Drosophila melanogaster, suggesting a dual neurotoxic mode of action. Overall, C. spinosa VOCs represent promising natural candidates for sustainable bioherbicide and bioinsecticide development.

在寻找合成农药的环保替代品的过程中，Calicotome spinosa （L.）的挥发性有机化合物（VOCs）研究了地中海灌木林克的药用和生态价值。ITS测序结果表明，与标准物质（accession PX285687）具有较高的相似性。本研究旨在通过固相微萃取-气相色谱-质谱联用技术（SPME-GC-MS）对植物挥发物进行表征，并在硅分子对接的支持下评估其植物毒性和杀虫性能。SPME-GC-MS分析鉴定出20种挥发性有机化合物，主要成分为六氢法尼基丙酮（33.6%）、二氢actinidiolide（13.4%）、反式β-离子酮（12.7%）、法尼烷（6.0%）和3,5-二叔丁基苯酚（5.6%）。生物实验表明，正己烷和二氯甲烷提取物对蓝锥虫表现出强烈的剂量依赖性植物毒性，显著抑制发芽和幼苗生长。杀虫试验表明，二氯甲烷提取物具有较强的熏蒸活性：72 h内，对虎尾剑虫和库氏Ephestia的致死率分别为100%和90%，而正己烷的致死率略低。残余毒性证实了不同治疗的死亡率一致。分子对接表明，六氢法尼酯丙酮和3,5-二叔丁基苯酚与黑腹果蝇乙酰胆碱酯酶（AChE）和章鱼胺受体（Oamb）有很强的结合，表明其具有双重神经毒性。综上所述，棘草挥发性有机化合物是开发可持续生物除草剂和生物杀虫剂的天然候选物质。

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引用次数: 0

Purification and characterization of chitinase from Cellulosimicrobium cellulans RS7: Insights into its antimicrobial and anticancer potential 从纤维素微生物RS7中分离纯化几丁质酶及其抑菌和抗癌潜力

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-10 DOI: 10.1016/j.bcab.2026.103925

Gaytri Mahajan, Vivek Chauhan, Rakesh Kumar, Reena Gupta

Chitinase produced by Cellulosimicrobium cellulans RS7 was purified up to 17.28-fold with a yield of 7.02 % and specific activity of 426.9 U/mg. In both Native and SDS-PAGE, the purified chitinase had a single band (47 kDa). It showed maximum activity at 35 °C with sodium phosphate buffer (0.5 M, pH 7.5) in the presence of substrate colloidal chitin (2.0 %) and Mg²⁺ ions. The kinetic studies showed that chitinase had Km and Vmax value of 1.14 mg/mL and 476.19 μmol mg⁻¹ min⁻¹, respectively. Furthermore, 3-D structure of the purified chitinase determined by MALDI- TOF MS technique revealed three active site residues: glutamic acid 292, 296 and aspartic acid 297. The purified chitinase showed cytotoxicity towards RD and L20B cell lines by effectively inhibiting cell growth. Moreover, chitinase produced by C. cellulans RS7 showed low hemolytic activity towards red blood cells. Chitinase also showed antimicrobial activity against S. aureus, S. flexneri and Candida albicans with zone of inhibition 30, 30 and 29 mm respectively.

由cellulosimmicroum cellulans RS7产生的几丁质酶纯化率为17.28倍，产率为7.02%，比活性为426.9 U/mg。在Native和SDS-PAGE中，纯化的几丁质酶具有单条带（47 kDa）。在35°C磷酸钠缓冲液（0.5 M, pH 7.5）下，底物胶体几丁质（2.0 %）和Mg2+离子存在时，其活性最大。动力学研究表明，几丁质酶的Km和Vmax分别为1.14 mg/mL和476.19 μmol mg−1 min−1。此外，利用MALDI- TOF质谱技术测定了纯化几丁质酶的三维结构，发现了三个活性位点残基：谷氨酸292、296和天冬氨酸297。纯化的几丁质酶对RD和L20B细胞株表现出细胞毒性，能有效抑制细胞生长。此外，C. cellulans RS7产生的几丁质酶对红细胞的溶血活性较低。几丁质酶对金黄色葡萄球菌、弗氏葡萄球菌和白色念珠菌的抑制区分别为30、30和29 mm。

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引用次数: 0

Bio-stimulants role in modulating the physiological attributes and antioxidant defense system for improving the growth and productivity of flax plants 生物刺激剂在调节亚麻植物生理特性和抗氧化防御系统中的作用，促进亚麻植物的生长和生产力

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-09 DOI: 10.1016/j.bcab.2026.103929

A.S.M. Younis , A.A. Ramadan , M.M.S. Abdallah , B.A. Bakry , H.M.S. El-Bassiouny

A field experiment was conducted at the Research and Production Station of the National Research Centre (NRC), Al-Nubaria District, Al-Behaira Governorate, Egypt, during two successive winter seasons, 2021/2022 and 2022/2023. To estimate the effects of foliar application of alanine and biotin (50, 75, and 100 mg/L) on flax (Linum usitatissimum L.; cultivar Sakha-3), growth characteristics, photosynthetic pigments, seed yield quantity and quality, and some biochemical aspects, grown in sandy soil. Exogenous application of alanine or biotin improved flax growth and yield by stimulating the biosynthesis of indole-3-acetic acid (IAA), enhanced the activities of key antioxidant enzymes (peroxidase, polyphenol oxidase, superoxide dismutase, and ascorbate peroxidase), and increased the levels of non-enzymatic antioxidant compounds such as phenols, flavonoids, β-carotene, lycopene, anthocyanins, and glutathione. Moreover, alanine and biotin concentrations caused significant increases in nutritional values of the yielded seeds as carbohydrates, proteins, and oil percentage. The produced seeds had a higher ratio of unsaturated to saturated fatty acids. It was observed that 75 mg/L of alanine induced the highest % of increase compared to control plants in terms of No. of capsules/plant (44.26 %), capsules yield/plant (70.89 %), seeds yield/plant (40 %), No. of seeds/capsule (36.01 %). Consequently the quantity and quality of seed yield (ton/ha), and oil yield (ton/ha) of flax plants grown in reclaimed sandy soil were increased. Meanwhile, biotin at 100 mg/L improved the quality of oil in terms of unsaturated/saturated fatty acid ratio compared to the control, being 8.03 vs. 3.72 %, respectively.

在2021/2022年和2022/2023年连续两个冬季，在埃及Al-Behaira省Al-Nubaria区国家研究中心（NRC）的研究和生产站进行了现场试验。研究了叶面施用丙氨酸和生物素（50、75和100 mg/L）对沙质土壤中亚麻（Linum usitatissimum L.; Sakha-3）生长特性、光合色素、籽粒产量和品质及一些生化指标的影响。外源应用丙氨酸或生物素通过刺激吲哚-3-乙酸（IAA）的生物合成，提高关键抗氧化酶（过氧化物酶、多酚氧化酶、超氧化物歧化酶和抗坏血酸过氧化物酶）的活性，提高非酶抗氧化化合物（如酚类、类黄酮、β-胡萝卜素、番茄红素、花青素和谷胱甘肽）的水平，促进亚麻生长和产量。此外，丙氨酸和生物素浓度显著提高了所产种子的碳水化合物、蛋白质和油脂的营养价值。所生产的种子中不饱和脂肪酸与饱和脂肪酸的比例较高。结果表明，与对照植株相比，75 mg/L丙氨酸诱导的氮素含量的增加百分比最高。荚果数/株（44.26%）、荚果数/株（70.89%）、种子数/株（40%）；种子/蒴果占36.01%。结果表明，在复垦砂质土壤上种植的亚麻种子产量（吨/公顷）和油料产量（吨/公顷）的数量和质量均有所提高。同时，生物素浓度为100 mg/L时，与对照相比，不饱和脂肪酸/饱和脂肪酸比值提高了油脂质量，分别为8.03和3.72%。

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引用次数: 0

Enzymatic hydrolysis of poly(lactic acid) by lipase and protease from Bacillus safensis 萨芬芽孢杆菌脂肪酶和蛋白酶水解聚乳酸的研究

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-09 DOI: 10.1016/j.bcab.2026.103930

Chunwang Li , Yujun Wang , Hanzhong Liang , Rui Cheng , Yuanyuan Wang , Zhanyong Wang

The degradation of polylactic acid (PLA) by triacylglycerol lipase (TGL) and S8 family serine peptidase (S8SP), which were identified in Bacillus safensis and heterologously expressed in Escherichia coli, was investigated in this study. The degradation of PLA films by these two enzymes was investigated using scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and gel permeation chromatography (GPC). TGL, S8SP, and their mixed enzymes resulted in PLA emulsion clarification and PLA film weight loss, and the mixed enzymes had better results. This implies that there is a synergistic effect between the two enzymes in the PLA degradation process. The degradation of the PLA films by both enzymes was due to surface erosion. PLA films treated with TGL exhibited irregular erosion on their surfaces, whereas the films treated with S8SP exhibited streak-like erosion. Enzymatic degradation leads to an increase in the number of hydrophilic groups on the surface, which in turn leads to an increase in the hydrophilicity of PLA films. In addition, the enzyme-treated PLA films showed a decrease in crystallinity and relative molecular weight, which was related to the degradation of the amorphous regions of PLA and the conversion of the crystalline regions into amorphous regions. The synergistic effect of TGL and S8SP in PLA degradation is an important reason for the ability of the source microorganism Bacillus safensis to effectively degrade PLA.

本研究研究了三酰甘油脂肪酶（TGL）和S8家族丝氨酸肽酶（S8SP）对聚乳酸（PLA）的降解作用，这两种酶分别在萨菲芽孢杆菌中鉴定，并在大肠杆菌中异种表达。采用扫描电镜（SEM）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）、x射线衍射（XRD）、热重分析（TGA）、差示扫描量热法（DSC）和凝胶渗透色谱（GPC）研究了这两种酶对PLA薄膜的降解作用。TGL、S8SP及其混合酶对聚乳酸乳液的澄清和聚乳酸膜的减重效果较好。这表明在聚乳酸降解过程中，两种酶之间存在协同作用。两种酶对聚乳酸膜的降解是由于表面侵蚀。经TGL处理的PLA膜表面呈现不规则的侵蚀，而经S8SP处理的PLA膜表面呈现条状侵蚀。酶降解导致表面亲水性基团数量增加，这反过来又导致PLA膜的亲水性增加。此外，酶处理后的PLA薄膜结晶度和相对分子量下降，这与PLA的非晶态区被降解，结晶区转化为非晶态区有关。TGL和S8SP在PLA降解中的协同作用是源微生物萨芬芽孢杆菌能够有效降解PLA的重要原因。

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引用次数: 0

Development of chitosanases of Lecanicillium lecanii in pilot-scale submerged cultures for production of low molecular weight chitosan and chitooligosaccharides 中试潜水培养生产低分子量壳聚糖和壳寡糖的lecanicillum壳聚糖酶的开发

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-08 DOI: 10.1016/j.bcab.2026.103927

Jennyfer Hernández-Alcántara , Jesús Rojas-Osnaya , Makoto Anraku , Hideaki Nakamura , Keiko Shirai

Chitosan with a degree of acetylation of 18.15 % at concentrations of 5, 10, and 15 g L⁻¹ was used as an inducer and sole carbon source in Czapek medium for chitosanase production by Lecanicillium lecanii in 3 L bioreactor submerged cultures. Subsequently, chitosanase production was scaled up to 50 L in submerged fermentation using the selected chitosan concentration (10 g L⁻¹) added to the culture medium, while maintaining key operational parameters, such as pH, temperature, and volumetric oxygen mass transfer coefficient. The enzymes were purified by salting-out, molecular size exclusion, and anionic exchange chromatography. The chitosanases presented molecular weights of 31 and 44 kDa, with optimal reaction conditions of 50 °C and pH 6, yielding a specific chitosanase activity of 25.97 ± 0.43 U mg⁻¹. This activity enabled the enzymes to cleave substrates such as glycol chitosan, chitosan (degree of acetylation 18.15 %), and 4-methylumbelliferyl-N-acetyl-β-D-glucosamine. The chitosanases produced chitooligomers with 2 and 6 repeating units, as well as low molecular weight chitosan with molecular masses ranging from 8 to 102 kDa, and an increased degree of acetylation in COS. The degree of hydrolysis was controlled by the chitosanase concentration (purity grade) and the reaction time, allowing the production of chitosan derivatives on a pilot scale. The employment of chitosanases facilitates the production of partially acetylated oligosaccharides and low-molecular-weight chitosan, which has broad potential in industrial applications, including pharmaceuticals, agriculture, and food technology.

在Czapek培养基中，以乙酰化程度为18.15%的5、10和15 g L−1的壳聚糖作为诱诱剂和唯一碳源，研究了Lecanicillium lecanii在3l生物反应器中生产壳聚糖酶。随后，将选定的壳聚糖浓度（10 g L−1）添加到培养基中，在保持关键操作参数（如pH、温度和体积氧传质系数）的情况下，将壳聚糖酶的产量扩大到50 L。采用盐析法、分子量排除法和阴离子交换色谱法对酶进行纯化。壳聚糖酶的分子量分别为31和44 kDa，反应温度为50℃，pH为6时，壳聚糖酶的比活性为25.97±0.43 U mg−1。这种活性使酶能够裂解乙二醇壳聚糖、壳聚糖（乙酰化程度18.15%）和4-甲基伞形eryl- n -乙酰-β- d -葡萄糖胺等底物。壳聚糖酶可制得具有2个和6个重复单元的壳聚糖低聚物，以及分子量在8 ~ 102 kDa之间的低分子量壳聚糖，并提高了COS的乙酰化程度。水解程度由壳聚糖酶浓度（纯度等级）和反应时间控制，可在中试规模上生产壳聚糖衍生物。壳聚糖酶的应用促进了部分乙酰化低聚糖和低分子量壳聚糖的生产，在医药、农业和食品技术等工业领域具有广泛的应用潜力。

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引用次数: 0