Biocatalysis and agricultural biotechnology最新文献_第2页

Biopriming of Solanum lycopersicum seeds with novel root endophytic bacterial consortium retrieved from halotolerant Sundarban mangroves to sustain growth and yield with salt resilience 用从耐盐巽他红树林中提取的新型根内生细菌群对茄科植物种子进行生物修复，以维持其生长和产量，提高抗盐能力

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-23 DOI: 10.1016/j.bcab.2024.103414

Mangroves are often found in coastal areas and tropical wetlands that can withstand high salinity. We hypothesized that endophytes that are harbouring in the roots of mangrove plants may improve the innate immunity of host plants to survive naturally in saline environment. Retrieving these endophytes and sequential characterization may function as a novel bio-effector for non-host food crops as well. We focused on the integrated approach towards formulating a novel bacterial consortium. Thirty-one bacterial endophytes isolated from the roots of mangrove plants were screened for plant growth promoting potential by inoculating our model crop (Tomato). Seven most promising isolates impacting plant growth were identified. In-vitro plant growth promoting characters were also analysed. The root colonization by the isolates was confirmed by Scanning Electron Microscopy (SEM). Among the screened isolates, four of them were found to be compatible with each other when grown together and were selected to formulate a novel biostimulant consortia. The consortia treated Tomato plants exhibited superior phenological characters. In the pot experiment, plant height of the treated plants was about ≈43 cm while the non-treated plants under salt stress could grow only up to a height of ≈26 cm. Similarly, a total fruit yield of ≈6.8 kg was observed in case of treated plants under salt stress whereas the non-treated plants under salt stress could only produce ≈4.7 kg of fruit. This study demonstrated that the beneficial bacteria inhabiting in mangrove roots can increase the potential of conferring salt tolerance to non-host crops, thereby contributing to sustainable agriculture.

红树林通常生长在能承受高盐度的沿海地区和热带湿地。我们假设，红树植物根部的内生菌可以提高寄主植物的先天免疫力，使其在盐碱环境中自然生存。检索这些内生菌并对其进行连续鉴定，也可作为一种新型生物效应剂用于非寄主粮食作物。我们的重点是采用综合方法组建新型细菌联合体。通过接种我们的模式作物（番茄），筛选了从红树植物根部分离出来的 31 种细菌内生体，以确定其促进植物生长的潜力。最终确定了七种最有希望影响植物生长的分离菌。同时还分析了体外植物生长促进特性。通过扫描电子显微镜（SEM）确认了分离物的根部定殖。在筛选出的分离物中，发现其中四种在一起生长时相互兼容，因此被选为新型生物刺激剂联合体。经联合体处理过的番茄植株表现出优异的物候特征。在盆栽实验中，经处理的植株高度约为≈43 厘米，而在盐胁迫下未经处理的植株只能长到≈26 厘米。同样，在盐胁迫下，经处理的植株果实总产量≈6.8 千克，而在盐胁迫下未经处理的植株只能生产≈4.7 千克果实。这项研究表明，栖息在红树林根部的有益细菌可以提高非寄主作物的耐盐潜力，从而为可持续农业做出贡献。

{"title":"Biopriming of Solanum lycopersicum seeds with novel root endophytic bacterial consortium retrieved from halotolerant Sundarban mangroves to sustain growth and yield with salt resilience","authors":"","doi":"10.1016/j.bcab.2024.103414","DOIUrl":"10.1016/j.bcab.2024.103414","url":null,"abstract":"<div><div>Mangroves are often found in coastal areas and tropical wetlands that can withstand high salinity. We hypothesized that endophytes that are harbouring in the roots of mangrove plants may improve the innate immunity of host plants to survive naturally in saline environment. Retrieving these endophytes and sequential characterization may function as a novel bio-effector for non-host food crops as well. We focused on the integrated approach towards formulating a novel bacterial consortium. Thirty-one bacterial endophytes isolated from the roots of mangrove plants were screened for plant growth promoting potential by inoculating our model crop (Tomato). Seven most promising isolates impacting plant growth were identified. <em>In-vitro</em> plant growth promoting characters were also analysed. The root colonization by the isolates was confirmed by Scanning Electron Microscopy (SEM). Among the screened isolates, four of them were found to be compatible with each other when grown together and were selected to formulate a novel biostimulant consortia. The consortia treated Tomato plants exhibited superior phenological characters. In the pot experiment, plant height of the treated plants was about ≈43 cm while the non-treated plants under salt stress could grow only up to a height of ≈26 cm. Similarly, a total fruit yield of ≈6.8 kg was observed in case of treated plants under salt stress whereas the non-treated plants under salt stress could only produce ≈4.7 kg of fruit. This study demonstrated that the beneficial bacteria inhabiting in mangrove roots can increase the potential of conferring salt tolerance to non-host crops, thereby contributing to sustainable agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accumulation of podophyllotoxin in the root culture of Hyptis suaveolens (L.) Poit: A potential natural lignan for clinically useful anticancer drugs 在Hyptis suaveolens（L.）Poit.根培养物中积累荚叶素：一种可用于临床抗癌药物的潜在天然木质素

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-19 DOI: 10.1016/j.bcab.2024.103416

Podophyllotoxin (PTOX) is a natural antiviral, antirheumatic and anticancer molecule, but it is expensive to chemically synthesize. The present study aimed to evaluate growth and PTOX accumulation in root cultures of Hyptis suaveolens treated with different concentrations of auxins, vitamins and myo-inositol. Root cultures grown in liquid medium supplemented with different concentrations of indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA), vitamins and myo-inositol were used to measure biomass production and PTOX content. PTOX quantitation was performed via high-performance liquid chromatography with diode-array detection (HPLC-DAD) using a newly developed analytical method after successful validation. Root culture in MS medium supplemented with 1 mg L⁻¹ IBA +0.5 mg L⁻¹ NAA resulted in the highest root dry weight (248.76 mg) and the highest PTOX concentration in the roots (179.97 μg g⁻¹ root). The roots cultured in liquid MS medium supplemented with 1 mg L⁻¹ IBA +0.5 mg L⁻¹ NAA presented the greatest increase in root biomass and PTOX content. This adequate balance of vitamin and myo-inositol supplementation in liquid MS culture medium increased the root dry weight and PTOX content.

鬼臼毒素（PTOX）是一种天然的抗病毒、抗风湿和抗癌分子，但其化学合成成本高昂。本研究旨在评估用不同浓度的辅助素、维生素和肌醇处理的半枝莲（Hyptis suaveolens）根培养物的生长和 PTOX 积累情况。在补充了不同浓度的吲哚-3-丁酸（IBA）、1-萘乙酸（NAA）、维生素和肌醇的液体培养基中生长的根培养物被用来测量生物量产量和 PTOX 含量。在成功验证后，采用新开发的分析方法，通过高效液相色谱-二极管阵列检测（HPLC-DAD）对 PTOX 进行了定量。在添加了 1 mg L-1 IBA +0.5 mg L-1 NAA 的 MS 培养基中培养的根干重最大（248.76 mg），根中的 PTOX 浓度最高（179.97 μg g-1 根）。在补充了 1 mg L-1 IBA +0.5 mg L-1 NAA 的液体 MS 培养基中培养的根，其根生物量和 PTOX 含量的增幅最大。液体 MS 培养基中维生素和肌醇的充分平衡补充增加了根的干重和 PTOX 含量。

{"title":"Accumulation of podophyllotoxin in the root culture of Hyptis suaveolens (L.) Poit: A potential natural lignan for clinically useful anticancer drugs","authors":"","doi":"10.1016/j.bcab.2024.103416","DOIUrl":"10.1016/j.bcab.2024.103416","url":null,"abstract":"<div><div>Podophyllotoxin (PTOX) is a natural antiviral, antirheumatic and anticancer molecule, but it is expensive to chemically synthesize. The present study aimed to evaluate growth and PTOX accumulation in root cultures of <em>Hyptis suaveolens</em> treated with different concentrations of auxins, vitamins and myo-inositol. Root cultures grown in liquid medium supplemented with different concentrations of indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA), vitamins and myo-inositol were used to measure biomass production and PTOX content. PTOX quantitation was performed via high-performance liquid chromatography with diode-array detection (HPLC-DAD) using a newly developed analytical method after successful validation. Root culture in MS medium supplemented with 1 mg L<sup>−1</sup> IBA +0.5 mg L<sup>−1</sup> NAA resulted in the highest root dry weight (248.76 mg) and the highest PTOX concentration in the roots (179.97 μg g<sup>−1</sup> root). The roots cultured in liquid MS medium supplemented with 1 mg L<sup>−1</sup> IBA +0.5 mg L<sup>−1</sup> NAA presented the greatest increase in root biomass and PTOX content. This adequate balance of vitamin and myo-inositol supplementation in liquid MS culture medium increased the root dry weight and PTOX content.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae to remove pharmaceutical and personal care products (PPCPs) from wastewater 用微藻类去除废水中的药物和个人护理产品 (PPCPs)

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-19 DOI: 10.1016/j.bcab.2024.103415

Substances used for medicinal, cosmetic, hygiene, and health care objectives are included in the category of pharmaceuticals and personal care products (PPCPs). They are a major source of public concern because of their ubiquitous manufacturing, usage, and careless disposal of expired products into the environment. Many PPCPs, including antibiotics, analgesics, endocrine disruptors, and microbial/disinfecting agents, are commonly detected in freshwater systems, groundwater, and wastewater treatment effluents in amounts ranging from nanograms per liter to milligrams per milliliter. Additionally, these compounds frequently show persistence and accumulate in biological tissue, eventually finding their way into crops, vegetables, and drinking water supplies. Because many PPCPs are known to have the ability to upset ecosystems and/or provide health hazards, they are categorized as “emerging contaminants.” The research on the occurrence, fate, and possible health and environmental hazards related to PPCPs in both aquatic and terrestrial habitats is thoroughly reviewed in this work. It also covers reported cases of danger or health concerns in humans, although a full assessment may not be possible given the limitations of the data at hand. This review aims to provide a comprehensive and well-focused overview of the current understanding of bioremediation strategies for the removal of pharmaceuticals and personal care products (PPCPs), with an emphasis on the application of macroalgae, microalgae, and aquatic macrophytes. It examines the physicochemical properties of PPCPs and their potential risks to environmental and human health. Additionally, the review explores the potential and challenges associated with the broader application of biotechnologies employing algae and aquatic macrophytes. This includes research efforts to correlate the operational parameters of these biotechnologies with the primary mechanisms responsible for PPCP removal. In conclusion, algae and macrophytes present promising, eco-friendly solutions for wastewater treatment, significantly contributing to the mitigation of PPCP contamination.

用于医药、化妆品、卫生和保健目的的物质都属于药品和个人护理产品（PPCPs）的范畴。由于其无处不在的生产、使用以及不小心将过期产品丢弃到环境中，它们成为公众关注的主要问题。在淡水系统、地下水和废水处理废水中，通常会检测到许多 PPCPs，包括抗生素、镇痛剂、内分泌干扰物和微生物/消毒剂，其含量从每升毫微克到每毫升毫克不等。此外，这些化合物通常具有持久性，会在生物组织中积累，最终进入农作物、蔬菜和饮用水源。由于许多 PPCPs 具有扰乱生态系统和/或危害健康的能力，因此被归类为 "新兴污染物"。本著作全面回顾了与水生和陆生栖息地中的 PPCPs 有关的发生、归宿以及可能的健康和环境危害方面的研究。虽然由于现有数据的局限性，可能无法进行全面评估，但本综述也涵盖了所报告的对人类造成危害或健康问题的案例。本综述旨在全面、重点突出地概述目前对去除药物和个人护理产品（PPCPs）的生物修复策略的理解，重点是大型藻类、微藻和水生大型藻类的应用。它研究了 PPCPs 的物理化学特性及其对环境和人类健康的潜在风险。此外，本综述还探讨了与更广泛应用藻类和水生大型藻类生物技术相关的潜力和挑战。这包括将这些生物技术的操作参数与去除 PPCP 的主要机制联系起来的研究工作。总之，藻类和大型水生植物为废水处理提供了前景广阔的生态友好型解决方案，大大有助于减轻 PPCP 污染。

{"title":"Microalgae to remove pharmaceutical and personal care products (PPCPs) from wastewater","authors":"","doi":"10.1016/j.bcab.2024.103415","DOIUrl":"10.1016/j.bcab.2024.103415","url":null,"abstract":"<div><div>Substances used for medicinal, cosmetic, hygiene, and health care objectives are included in the category of pharmaceuticals and personal care products (PPCPs). They are a major source of public concern because of their ubiquitous manufacturing, usage, and careless disposal of expired products into the environment. Many PPCPs, including antibiotics, analgesics, endocrine disruptors, and microbial/disinfecting agents, are commonly detected in freshwater systems, groundwater, and wastewater treatment effluents in amounts ranging from nanograms per liter to milligrams per milliliter. Additionally, these compounds frequently show persistence and accumulate in biological tissue, eventually finding their way into crops, vegetables, and drinking water supplies. Because many PPCPs are known to have the ability to upset ecosystems and/or provide health hazards, they are categorized as “emerging contaminants.” The research on the occurrence, fate, and possible health and environmental hazards related to PPCPs in both aquatic and terrestrial habitats is thoroughly reviewed in this work. It also covers reported cases of danger or health concerns in humans, although a full assessment may not be possible given the limitations of the data at hand. This review aims to provide a comprehensive and well-focused overview of the current understanding of bioremediation strategies for the removal of pharmaceuticals and personal care products (PPCPs), with an emphasis on the application of macroalgae, microalgae, and aquatic macrophytes. It examines the physicochemical properties of PPCPs and their potential risks to environmental and human health. Additionally, the review explores the potential and challenges associated with the broader application of biotechnologies employing algae and aquatic macrophytes. This includes research efforts to correlate the operational parameters of these biotechnologies with the primary mechanisms responsible for PPCP removal. In conclusion, algae and macrophytes present promising, eco-friendly solutions for wastewater treatment, significantly contributing to the mitigation of PPCP contamination.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-prospecting fluoride tolerant bacteria for their optimistic contribution in instigating resilience against fluoride stress in Oryza sativa L. 对耐氟细菌进行生物勘探，以了解它们在促进蚕豆抗氟胁迫方面的乐观贡献

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-18 DOI: 10.1016/j.bcab.2024.103412

In recent years, the effects of fluoride (F) pollution in numerous ecosystems such as groundwater, soil, etc. Have become a major issue worldwide. This increase in F pollution is a direct consequence of the unbridled use of fertilizers in agricultural and several other human activities that require immediate and appropriate action. Therefore, this manuscript reveals important findings on the efficacy of bacteria isolated from agricultural fields in central Chhattisgarh in manifesting resistance to F and in reversing the F-induced oxidative damage in susceptible Oryza sativa L, (Var. MTU1010). Chronic exposure of Oryza sativa L. to sodium fluoride (NaF) (50 mg L⁻¹) severely impeded growth and various physiological parameters such as germination percentage, biomass and root and shoot length and stimulated the formation of reactive oxygen species (ROS), which enhanced electrolyte leakage and formation of cytotoxic products like malondialdehyde. To this end, potential bacterial strains, namely MT2A, MT3A, MT4A, and Du3A were isolated, screened for various plant growth promoting (PGP) traits and used to explore their efficiency to mitigate F toxicity in Oryza sativa L. in vivo. The seedlings inoculated with the bacterial strains showed significant development as evidenced by an increase in root and shoot length, biomass and chlorophyll content. Additionally, inoculation of these strains in combination with F stress significantly decreased oxidative stress by increasing the expression of protective genes encoding antioxidant enzymes and boosted agronomic traits remarkably. Overall, the manuscript demonstrates the pivotal role played by the isolated bacteria in abating ill effects of F in the Oryza sativa L. seedlings and proves their potential as protective bioagents against F stress.

近年来，氟化物（F）污染对地下水、土壤等众多生态系统的影响已成为世界性的重大问题。已成为全球范围内的一个主要问题。氟污染的增加是农业和其他一些人类活动中无节制使用化肥的直接后果，需要立即采取适当行动。因此，本手稿揭示了从恰蒂斯加尔邦中部农田中分离出的细菌在易感稻米（Oryza sativa L, (Var. MTU1010)）中表现出抗性和逆转 F 诱导的氧化损伤方面的重要发现。Oryza sativa L.长期暴露于氟化钠（NaF）（50 mg L-1）中会严重影响其生长和各种生理参数，如发芽率、生物量、根和芽的长度，并刺激活性氧（ROS）的形成，从而增加电解质的泄漏和丙二醛等细胞毒性产物的形成。为此，我们分离了潜在的细菌菌株（即 MT2A、MT3A、MT4A 和 Du3A），筛选了它们的各种植物生长促进（PGP）特性，并利用它们来探索其在体内减轻旱金莲（Oryza sativa L.）F 毒性的效率。接种了这些细菌菌株的幼苗表现出显著的生长发育，表现为根长和芽长、生物量和叶绿素含量的增加。此外，接种这些菌株与 F 胁迫相结合，通过增加编码抗氧化酶的保护基因的表达，显著降低了氧化胁迫，并明显改善了农艺性状。总之，该手稿证明了分离出的细菌在减轻雌花胁迫对禾本科植物幼苗的不良影响方面所起的关键作用，并证明了它们作为抗雌花胁迫的保护性生物试剂的潜力。

{"title":"Bio-prospecting fluoride tolerant bacteria for their optimistic contribution in instigating resilience against fluoride stress in Oryza sativa L.","authors":"","doi":"10.1016/j.bcab.2024.103412","DOIUrl":"10.1016/j.bcab.2024.103412","url":null,"abstract":"<div><div>In recent years, the effects of fluoride (F) pollution in numerous ecosystems such as groundwater, soil, <em>etc</em>. Have become a major issue worldwide. This increase in F pollution is a direct consequence of the unbridled use of fertilizers in agricultural and several other human activities that require immediate and appropriate action. Therefore, this manuscript reveals important findings on the efficacy of bacteria isolated from agricultural fields in central Chhattisgarh in manifesting resistance to F and in reversing the F-induced oxidative damage in susceptible <em>Oryza sativa</em> L, (Var. MTU1010). Chronic exposure of <em>Oryza sativa</em> L. to sodium fluoride (NaF) (50 mg L<sup>−1</sup>) severely impeded growth and various physiological parameters such as germination percentage, biomass and root and shoot length and stimulated the formation of reactive oxygen species (ROS), which enhanced electrolyte leakage and formation of cytotoxic products like malondialdehyde. To this end, potential bacterial strains, namely MT2A, MT3A, MT4A, and Du3A were isolated, screened for various plant growth promoting (PGP) traits and used to explore their efficiency to mitigate F toxicity in <em>Oryza sativa</em> L. <em>in vivo.</em> The seedlings inoculated with the bacterial strains showed significant development as evidenced by an increase in root and shoot length, biomass and chlorophyll content. Additionally, inoculation of these strains in combination with F stress significantly decreased oxidative stress by increasing the expression of protective genes encoding antioxidant enzymes and boosted agronomic traits remarkably. Overall, the manuscript demonstrates the pivotal role played by the isolated bacteria in abating ill effects of F in the <em>Oryza sativa</em> L. seedlings and proves their potential as protective bioagents against F stress.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Green integrative large scale treatment of tannery effluent, CO2 sequestration, and biofuel production using oleaginous green microalga Nannochloropsis oculata TSD05: An ecotechnological approach Title of article” [Biocatal. Agric. Biotechnol. 61 (2024) 103370] 对 "利用含油绿色微藻 Nannochloropsis oculata TSD05 对制革污水、二氧化碳封存和生物燃料生产进行绿色综合大规模处理：一种生态技术方法 "的更正文章标题[Biocatal. Agric. Biotechnol.

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-17 DOI: 10.1016/j.bcab.2024.103410

引用次数: 0

Co-metabolism of substrates by Pseudomonas aeruginosa NG4 regulates biosurfactant production 铜绿假单胞菌 NG4 对底物的共代谢调节生物表面活性剂的生产

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-01 DOI: 10.1016/j.bcab.2024.103382

As compared to chemical surfactants, biosurfactants exhibit a more significant impact in various sectors due to their degradability, stability, and other specific physicochemical properties. Biosurfactant production by Pseudomonas aeruginosa NG4 was studied by co-metabolism of 1% glucose (wv⁻¹) and 1% CG (vv⁻¹) with different time intervals. The addition of Glucose (0h) + CG (24 h) (1:1, wv⁻¹, vv⁻¹) to the culture medium resulted in 4.23 gL^-1 of biosurfactant production. An enhancement of about 4.75-fold in the yield was observed. The time period of incubation of CG in the feed was optimized to be 24 h after the initiation of fermentation with glucose. Among the several nitrogen sources used in this study, sodium nitrate produced more biosurfactant than the others. Biochemical characterization through FTIR and LC-MS of biosurfactant reflected the presence of mono- and di-rhamnolipids. Therefore, this study highlights the ability of P. aeruginosa NG4 to increase biosurfactant production by co-utilizing substrates.

与化学表面活性剂相比，生物表面活性剂因其可降解性、稳定性和其他特殊的物理化学特性，在各行各业中发挥着更为重要的作用。通过 1%葡萄糖（wv-1）和 1%CG（vv-1）在不同时间间隔内的共代谢，研究了铜绿假单胞菌 NG4 产生生物表面活性剂的情况。在培养基中加入葡萄糖（0 小时）+ CG（24 小时）（1:1，wv-1，vv-1）后，生物表面活性剂的产量为 4.23 gL-1。产量提高了约 4.75 倍。饲料中培养 CG 的时间优化为葡萄糖开始发酵后的 24 小时。在本研究使用的几种氮源中，硝酸钠比其他氮源产生更多的生物表面活性剂。通过傅立叶变换红外光谱和液相色谱-质谱对生物表面活性剂进行生化鉴定，发现其中含有单鼠李糖脂和双鼠李糖脂。因此，本研究强调了铜绿微囊藻 NG4 通过共同利用底物来提高生物表面活性剂产量的能力。

引用次数: 0

Development of method and analysis protocol for fatty acids derivatives of castor oil by using gas chromatography-mass spectrometry 利用气相色谱-质谱法开发蓖麻油脂肪酸衍生物的方法和分析规程

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-01 DOI: 10.1016/j.bcab.2024.103408

Castor oil is a vegetable oil extracted from castor beans, known for its diverse applications across the chemical industry, food industry, skincare products, biodiesel components, and pharmaceuticals. Analyzing the fatty acid composition of castor oil using Gas Chromatography-Mass Spectrometry (GC-MS) can be complex due to various factors inherent to fatty acids and the oil's composition. Therefore, the goal of this study is to effectively separate and analyze the major fatty acids present in castor oil using GC-MS. Castor oil was methylated using alcoholic potassium hydroxide, then neutralized with salicylic acid and centrifuged. The supernatant was diluted and injected into the GC-MS with a developed temperature program. The results indicated that castor oil contains five fatty acids: ricinoleic acid methyl ester, oleic acid methyl ester, linoleic acid methyl ester, stearic acid methyl ester, and palmitic acid methyl ester, with respective concentrations of 87.63%, 1.81%, 6.57%, 2.93%, and 1.07%. Among these, methyl ricinoleate was the most abundant at approximately 87.36%, while palmitic acid methyl ester had the lowest concentration at 1.07%. The order of fatty acids detected was methyl ricinoleate > linoleic acid > stearic acid > oleic acid methyl ester > palmitic acid. To our knowledge, this is the first report of an easy and efficient method for the analysis of castor oil. In conclusion, the developed methylation of castor oil and gas chromatography temperature program are suitable for the routine analysis of castor oil.

蓖麻油是从蓖麻中提取的一种植物油，因其在化学工业、食品工业、护肤品、生物柴油成分和制药等领域的广泛应用而闻名。使用气相色谱-质谱法（GC-MS）分析蓖麻油的脂肪酸组成可能会很复杂，这是因为脂肪酸和油的组成存在各种固有因素。因此，本研究的目标是利用气相色谱-质谱法有效地分离和分析蓖麻油中的主要脂肪酸。蓖麻油使用氢氧化钾酒精进行甲基化，然后用水杨酸中和并离心。上清液经过稀释后，按照设定的温度程序注入气相色谱-质谱仪。结果表明，蓖麻油中含有五种脂肪酸：蓖麻油酸甲酯、油酸甲酯、亚油酸甲酯、硬脂酸甲酯和棕榈酸甲酯，其浓度分别为 87.63%、1.81%、6.57%、2.93% 和 1.07%。其中，蓖麻油酸甲酯的含量最高，约为 87.36%，而棕榈酸甲酯的含量最低，仅为 1.07%。检测到的脂肪酸顺序为：蓖麻油酸甲酯；亚油酸；硬脂酸；油酸甲酯；棕榈酸。据我们所知，这是首次报道用于分析蓖麻油的简便而高效的方法。总之，所开发的蓖麻油甲基化和气相色谱温度程序适用于蓖麻油的常规分析。

{"title":"Development of method and analysis protocol for fatty acids derivatives of castor oil by using gas chromatography-mass spectrometry","authors":"","doi":"10.1016/j.bcab.2024.103408","DOIUrl":"10.1016/j.bcab.2024.103408","url":null,"abstract":"<div><div>Castor oil is a vegetable oil extracted from castor beans, known for its diverse applications across the chemical industry, food industry, skincare products, biodiesel components, and pharmaceuticals. Analyzing the fatty acid composition of castor oil using Gas Chromatography-Mass Spectrometry (GC-MS) can be complex due to various factors inherent to fatty acids and the oil's composition. Therefore, the goal of this study is to effectively separate and analyze the major fatty acids present in castor oil using GC-MS. Castor oil was methylated using alcoholic potassium hydroxide, then neutralized with salicylic acid and centrifuged. The supernatant was diluted and injected into the GC-MS with a developed temperature program. The results indicated that castor oil contains five fatty acids: ricinoleic acid methyl ester, oleic acid methyl ester, linoleic acid methyl ester, stearic acid methyl ester, and palmitic acid methyl ester, with respective concentrations of 87.63%, 1.81%, 6.57%, 2.93%, and 1.07%. Among these, methyl ricinoleate was the most abundant at approximately 87.36%, while palmitic acid methyl ester had the lowest concentration at 1.07%. The order of fatty acids detected was methyl ricinoleate > linoleic acid > stearic acid > oleic acid methyl ester > palmitic acid. To our knowledge, this is the first report of an easy and efficient method for the analysis of castor oil. In conclusion, the developed methylation of castor oil and gas chromatography temperature program are suitable for the routine analysis of castor oil.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential oils from plant resources as potent insecticides and repellents: Current status and future perspectives 从植物资源中提取精油作为强效杀虫剂和驱虫剂：现状与未来展望

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-01 DOI: 10.1016/j.bcab.2024.103395

The extensive use of chemical pesticides on crop plants is harmful to mankind as well as to insect predators. Their long-term usage contaminates water, soil, and the food chain with the accumulation of toxic residues. Plant-based essential oils with a broad spectrum of antifungal, larvicidal, ovicidal, and pesticidal activities are now being preferred as an alternative and sustainable source to safeguard crop plants. Evidence now exists that essential oils alter the nervous and locomotor activities of insects. They target specific and may affect the various systems of the insect pests. This may generally do no or less harm to mammals including humans when compared to chemical insect pests, therefore they can be deployed as relatively safer molecules to reign in the devastating pests and reduce economic losses. They are cost-effective, available throughout the year, non-persistent in the environment, and vertebrate or non-target toxicity is minimal in comparison with chemical pesticides. This brief review focuses on the current status of essential oils used as potent pesticides, stored-product insect pest reduction, insect repellents, and fungicides. This also suggests the way forward to design superior strategies such as the technique of nano-encapsulation to ameliorate the stability and stave off their volatilization.

在农作物上大量使用化学农药对人类和昆虫捕食者都是有害的。长期使用会积累有毒残留物，污染水源、土壤和食物链。以植物为基础的精油具有广泛的抗真菌、杀幼虫剂、杀卵剂和杀虫剂活性，目前已成为保护农作物的可持续替代来源。现在有证据表明，精油会改变昆虫的神经和运动活动。它们具有特定的目标，可能会影响害虫的各种系统。与化学虫害相比，精油一般不会或较少对哺乳动物（包括人类）造成伤害，因此可以作为相对更安全的分子来控制破坏性害虫，减少经济损失。与化学杀虫剂相比，它们成本效益高、全年可用、在环境中无持久性，而且对脊椎动物或非靶标的毒性极小。这篇简短的综述重点介绍了香精油作为强效杀虫剂、储藏产品害虫消杀剂、驱虫剂和杀菌剂的现状。这也为设计更优越的策略提出了建议，如采用纳米包囊技术来改善稳定性和防止挥发。

{"title":"Essential oils from plant resources as potent insecticides and repellents: Current status and future perspectives","authors":"","doi":"10.1016/j.bcab.2024.103395","DOIUrl":"10.1016/j.bcab.2024.103395","url":null,"abstract":"<div><div>The extensive use of chemical pesticides on crop plants is harmful to mankind as well as to insect predators. Their long-term usage contaminates water, soil, and the food chain with the accumulation of toxic residues. Plant-based essential oils with a broad spectrum of antifungal, larvicidal, ovicidal, and pesticidal activities are now being preferred as an alternative and sustainable source to safeguard crop plants. Evidence now exists that essential oils alter the nervous and locomotor activities of insects. They target specific and may affect the various systems of the insect pests. This may generally do no or less harm to mammals including humans when compared to chemical insect pests, therefore they can be deployed as relatively safer molecules to reign in the devastating pests and reduce economic losses. They are cost-effective, available throughout the year, non-persistent in the environment, and vertebrate or non-target toxicity is minimal in comparison with chemical pesticides. This brief review focuses on the current status of essential oils used as potent pesticides, stored-product insect pest reduction, insect repellents, and fungicides. This also suggests the way forward to design superior strategies such as the technique of nano-encapsulation to ameliorate the stability and stave off their volatilization.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-synthesized AGS@AgNPs for wound healing, antioxidant support, antibacterial defense, and anticancer intervention 用于伤口愈合、抗氧化、抗菌和抗癌干预的生物合成 AGS@AgNPs

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-01 DOI: 10.1016/j.bcab.2024.103402

Slow wound healing and wound infections are significant challenges for burn patients. Biosynthesis of nanoparticles (NPs) using plant extracts offers a rapid, facile, and safe method for producing biocompatible NPs. In this study, Anethum graveolens seed (AGS) extract served as a masking and reducing agent for the synthesis of silver nanoparticles (AGS@AgNPs). Initially, the parameters influencing AGS@AgNPs synthesis, including silver nitrate concentration, reaction time, temperature, and pH, were optimized. Subsequently, AGS@AgNPs structural and biological characteristics were evaluated. Observation of the surface plasmon resonance (SPR) of the AGS@AgNPs at approximately 420 nm, accompanied by a color change of the suspension to dark brown, confirmed AGS@AgNPs synthesis. Analysis via X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta potential, and transmission electron microscopy (TEM) confirmed the production of pure, homogeneous, spherical, and stable silver NPs with sizes ranging from 20 to 40 nm using Anethum graveolens extract. Antibacterial assays revealed significant activity against both Gram-positive and Gram-negative bacterial strains. Antioxidant assessments demonstrated that AGS@AgNPs at a concentration of 250 μg/ml exhibited 92% inhibition of DPPH free radicals and in FRAP test, A. graveolens seed extract and AGS@AgNPs reduced Fe³⁺ ions to Fe²⁺ by 48.7% and 73.1%, respectively. Investigation of anticancer properties against the lung cancer cell line (A-549) revealed an IC₅₀ value of 242 μg/ml. The results of the flow cytometry test and lactate dehydrogenase (LDH) assay demonstrated the death of cancer cells. Moreover, wound healing assays demonstrated a significant acceleration in burn wound closure rates in rats on days 3, 7, and 14 following treatments with AGS@AgNPs. Overall, these findings highlight the favorable biological activities of AGS@AgNPs, suggesting their potential utility in nanomedicine applications.

伤口愈合缓慢和伤口感染是烧伤患者面临的重大挑战。利用植物提取物进行纳米粒子（NPs）的生物合成提供了一种快速、简便、安全的生物相容性 NPs 生产方法。在本研究中，Anethum graveolens seed（AGS）提取物作为掩蔽剂和还原剂用于合成银纳米粒子（AGS@AgNPs）。首先，优化了影响 AGS@AgNPs 合成的参数，包括硝酸银浓度、反应时间、温度和 pH 值。随后，对 AGS@AgNPs 的结构和生物特性进行了评估。在约 420 纳米波长处观察到 AGS@AgNPs 的表面等离子体共振（SPR），同时悬浮液的颜色变为深棕色，这证实了 AGS@AgNPs 的合成。通过 X 射线衍射 (XRD)、傅立叶变换红外光谱 (FT-IR)、动态光散射 (DLS)、ZETA 电位和透射电子显微镜 (TEM) 分析，证实了使用 Anethum graveolens 提取物生产出了纯净、均质、球形和稳定的银 NPs，其尺寸范围为 20 至 40 nm。抗菌试验显示，该产品对革兰氏阳性和革兰氏阴性细菌菌株均有显著的抗菌活性。抗氧化评估表明，浓度为 250 μg/ml 的 AGS@AgNPs 对 DPPH 自由基的抑制率为 92%；在 FRAP 试验中，A. graveolens 种子提取物和 AGS@AgNPs 将 Fe3+ 离子还原为 Fe2+ 的比率分别为 48.7% 和 73.1%。对肺癌细胞株（A-549）抗癌特性的研究表明，其 IC50 值为 242 μg/ml。流式细胞仪检测和乳酸脱氢酶（LDH）检测结果表明，癌细胞已经死亡。此外，伤口愈合试验表明，在使用 AGS@AgNPs 治疗后的第 3、7 和 14 天，大鼠烧伤伤口的闭合速度明显加快。总之，这些研究结果突显了 AGS@AgNPs 的良好生物活性，表明其在纳米医学应用中具有潜在的实用性。

{"title":"Bio-synthesized AGS@AgNPs for wound healing, antioxidant support, antibacterial defense, and anticancer intervention","authors":"","doi":"10.1016/j.bcab.2024.103402","DOIUrl":"10.1016/j.bcab.2024.103402","url":null,"abstract":"<div><div>Slow wound healing and wound infections are significant challenges for burn patients. Biosynthesis of nanoparticles (NPs) using plant extracts offers a rapid, facile, and safe method for producing biocompatible NPs. In this study, <em>Anethum graveolens</em> seed (AGS) extract served as a masking and reducing agent for the synthesis of silver nanoparticles (AGS@AgNPs). Initially, the parameters influencing AGS@AgNPs synthesis, including silver nitrate concentration, reaction time, temperature, and pH, were optimized. Subsequently, AGS@AgNPs structural and biological characteristics were evaluated. Observation of the surface plasmon resonance (SPR) of the AGS@AgNPs at approximately 420 nm, accompanied by a color change of the suspension to dark brown, confirmed AGS@AgNPs synthesis. Analysis via X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta potential, and transmission electron microscopy (TEM) confirmed the production of pure, homogeneous, spherical, and stable silver NPs with sizes ranging from 20 to 40 nm using Anethum graveolens extract. Antibacterial assays revealed significant activity against both Gram-positive and Gram-negative bacterial strains. Antioxidant assessments demonstrated that AGS@AgNPs at a concentration of 250 μg/ml exhibited 92% inhibition of DPPH free radicals and in FRAP test, <em>A. graveolens</em> seed extract and AGS@AgNPs reduced Fe<sup>3+</sup> ions to Fe<sup>2+</sup> by 48.7% and 73.1%, respectively. Investigation of anticancer properties against the lung cancer cell line (A-549) revealed an IC<sub>50</sub> value of 242 μg/ml. The results of the flow cytometry test and lactate dehydrogenase (LDH) assay demonstrated the death of cancer cells. Moreover, wound healing assays demonstrated a significant acceleration in burn wound closure rates in rats on days 3, 7, and 14 following treatments with AGS@AgNPs. Overall, these findings highlight the favorable biological activities of AGS@AgNPs, suggesting their potential utility in nanomedicine applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative approaches to combating dental biofilms: Nanotechnology and its applications 对抗牙科生物膜的创新方法：纳米技术及其应用

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2024-10-01 DOI: 10.1016/j.bcab.2024.103406

Dental biofilms, impacting around 80% of the global population, are a significant issue causing serious dental disorders like periodontitis, which has links to autoimmune diseases such as rheumatoid arthritis. Traditional biofilm management methods, including mechanical removal and chemical agents, often fail to completely eradicate biofilms, allowing oral infections to persist. Nanotechnology offers innovative solutions for medication delivery and biofilm disruption, presenting new opportunities for antimicrobial surfaces and healthcare equipment development. This paper explores advancements in nanotechnology-based strategies to combat dental biofilms, primarily formed by Lactobacillus species, Actinomyces species, and Streptococcus mutans, and examines the composition and characteristics of dental biofilms. The green synthesis of nanoparticles using plant-based ingredients demonstrates significant antibacterial activity against biofilm-associated pathogens. Additionally, the paper investigates the potential of various metal oxide-based nanoparticles, such as titanium, zinc, and silver, in dental treatments to inhibit biofilm formation and enhance oral health. The stages of dental biofilm formation are outlined, highlighting the risks of untreated biofilms, including systemic health implications. This review underscores the promise of nanodrugs in preventing dental biofilms and improving the efficacy of dental procedures. It delves into cutting-edge nanotechnological approaches, emphasizing the novel use of nanoparticles like gold, silver, and antimicrobial peptides, and their unique mechanisms for disrupting biofilm formation and enhancing dental treatments. The review also covers recent advancements in nanotechnology, offering innovative and more effective solutions for targeted dental care strategies, potentially revolutionizing the approach to managing dental biofilms and promoting better oral health outcomes.

牙科生物膜影响着全球约 80% 的人口，是导致牙周炎等严重牙科疾病的一个重要问题，而牙周炎又与类风湿性关节炎等自身免疫性疾病有关。传统的生物膜管理方法，包括机械清除和化学制剂，往往无法彻底根除生物膜，导致口腔感染持续存在。纳米技术为药物输送和生物膜破坏提供了创新的解决方案，为抗菌表面和医疗保健设备的开发带来了新的机遇。本文探讨了基于纳米技术的牙科生物膜（主要由乳酸杆菌、放线菌和变异链球菌形成）防治策略的进展，并研究了牙科生物膜的组成和特征。利用植物成分绿色合成的纳米粒子对生物膜相关病原体具有显著的抗菌活性。此外，论文还研究了各种基于金属氧化物的纳米粒子（如钛、锌和银）在牙科治疗中抑制生物膜形成和增进口腔健康的潜力。文章概述了牙科生物膜形成的各个阶段，强调了未经处理的生物膜的风险，包括对全身健康的影响。本综述强调了纳米药物在预防牙科生物膜和提高牙科治疗效果方面的前景。它深入探讨了最前沿的纳米技术方法，强调了金、银和抗菌肽等纳米粒子的新用途，以及它们破坏生物膜形成和提高牙科治疗效果的独特机制。该综述还涵盖了纳米技术的最新进展，为有针对性的牙科护理策略提供了创新和更有效的解决方案，有可能彻底改变牙科生物膜的管理方法，促进更好的口腔健康效果。

{"title":"Innovative approaches to combating dental biofilms: Nanotechnology and its applications","authors":"","doi":"10.1016/j.bcab.2024.103406","DOIUrl":"10.1016/j.bcab.2024.103406","url":null,"abstract":"<div><div>Dental biofilms, impacting around 80% of the global population, are a significant issue causing serious dental disorders like periodontitis, which has links to autoimmune diseases such as rheumatoid arthritis. Traditional biofilm management methods, including mechanical removal and chemical agents, often fail to completely eradicate biofilms, allowing oral infections to persist. Nanotechnology offers innovative solutions for medication delivery and biofilm disruption, presenting new opportunities for antimicrobial surfaces and healthcare equipment development. This paper explores advancements in nanotechnology-based strategies to combat dental biofilms, primarily formed by <em>Lactobacillus</em> species, <em>Actinomyces</em> species, and <em>Streptococcus mutans</em>, and examines the composition and characteristics of dental biofilms. The green synthesis of nanoparticles using plant-based ingredients demonstrates significant antibacterial activity against biofilm-associated pathogens. Additionally, the paper investigates the potential of various metal oxide-based nanoparticles, such as titanium, zinc, and silver, in dental treatments to inhibit biofilm formation and enhance oral health. The stages of dental biofilm formation are outlined, highlighting the risks of untreated biofilms, including systemic health implications. This review underscores the promise of nanodrugs in preventing dental biofilms and improving the efficacy of dental procedures. It delves into cutting-edge nanotechnological approaches, emphasizing the novel use of nanoparticles like gold, silver, and antimicrobial peptides, and their unique mechanisms for disrupting biofilm formation and enhancing dental treatments. The review also covers recent advancements in nanotechnology, offering innovative and more effective solutions for targeted dental care strategies, potentially revolutionizing the approach to managing dental biofilms and promoting better oral health outcomes.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0