Biocatalysis and agricultural biotechnology最新文献_第3页

A novel broad-specificity GH51 α-L-arabinofuranosidase from Paenibacillus antarcticus that acts synergistically with other xylanolytic enzymes to completely saccharify arabinoxylans 来自南极芽孢杆菌的一种新型广谱GH51 α- l -阿拉伯糖醛酸苷酶与其他木聚糖水解酶协同作用，使阿拉伯糖醛酸苷完全糖化

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-07 DOI: 10.1016/j.bcab.2026.103926

Yesi Maysita , Gayatri Budhi Khrisnamurti , Bauke W. Dijkstra , Ni Nyoman Tri Puspaningsih , Ali Rohman

The genome of the psychrotolerant bacterium Paenibacillus antarcticus KACC 11469 contains a gene for an α-L-arabinofuranosidase. We report biochemical properties of the recombinant product of this gene (rPan-AF51), the first enzyme to be characterized from this bacterium. Sequence and structural analyses identified it as a member of glycoside hydrolase family 51. The enzyme showed optimal activity at pH 6.0 and was stable over the pH range of 6.0–10.0. It exhibited optimal activity at 35 °C and retained both stability and activity at or below this temperature, indicating cold adaptation. rPan-AF51 was highly sensitive to transition-metal ions and detergents, whereas main-group-metal ions, EDTA, β-mercaptoethanol, and ethanol had minimal effect, and monosaccharides caused weak inhibition. rPan-AF51 is an exo-acting enzyme with high activity towards 4-nitrophenyl-α-L-arabinofuranoside (4-NPA); kinetic analysis revealed K_m, V_max, and k_cat values of 0.44 mM, 313 U/mg, and 309 s⁻¹, respectively. Remarkably, the enzyme is able to hydrolyse α-1,2, α-1,3, and α-1,5-L-arabinofuranosidic bonds at the non-reducing termini of α-L-arabinose-containing saccharides. Among the polysaccharides tested, the enzyme was most efficient on debranched sugar beet arabinan (DBAR), followed by partially debranched wheat arabinoxylan (EDWAX) and sugar beet arabinan (ARAB), with k_cat/K_m values of 0.484, 0.346, and 0.240 mL mg⁻¹·s⁻¹, respectively. Finally, rPan-AF51 acted synergistically with β-xylanase and β-xylosidase in the complete saccharification of rye flour arabinoxylan (RAXY), a substrate that is otherwise recalcitrant to complete hydrolysis. These properties make rPan-AF51 a potential catalyst for industrial applications that operate at low to moderate temperatures.

耐寒细菌南极芽孢杆菌KACC 11469的基因组含有一个α- l -阿拉伯糖醛酸苷酶基因。我们报道了该基因的重组产物（rPan-AF51）的生化特性，这是该细菌中第一个被表征的酶。序列和结构分析表明该酶为糖苷水解酶家族51的成员。该酶在pH 6.0时活性最佳，在pH 6.0 ~ 10.0范围内保持稳定。它在35°C时表现出最佳活性，在35°C或更低的温度下保持稳定性和活性，表明其具有冷适应性。rPan-AF51对过渡金属离子和洗涤剂高度敏感，而主基金属离子、EDTA、β-巯基乙醇和乙醇对其影响较小，单糖对其抑制作用较弱。rPan-AF51是一种对4-硝基苯基-α- l-阿拉伯糖醛酸苷（4-NPA）具有高活性的外显作用酶；动力学分析显示，Km、Vmax和kcat值分别为0.44 mM、313 U/mg和309 s−1。值得注意的是，该酶能够水解α- l -阿拉伯糖糖非还原末端的α-1,2, α-1,3和α-1,5- l -阿拉伯糖醛酸键。该酶对脱支的阿拉伯甜菜多糖（DBAR）的降解效率最高，其次是部分脱支的阿拉伯小麦木聚糖（EDWAX）和阿拉伯甜菜多糖（ARAB），其kcat/Km值分别为0.484、0.346和0.240 mL mg−1·s−1。最后，rPan-AF51与β-木聚糖酶和β-木糖苷酶协同作用，使黑麦面粉阿拉伯木聚糖（RAXY）完全糖化，否则这种底物难以完全水解。这些特性使rPan-AF51成为在低至中等温度下工作的工业应用的潜在催化剂。

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引用次数: 0

Production of Trichoderma asperellum in a packed-bed reactor with antagonist activity against Fusarium 具有镰孢菌拮抗活性的填充床反应器生产曲霉木霉

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-06 DOI: 10.1016/j.bcab.2025.103916

Karina Romero-Hernandez, Gabriela Sepúlveda-Jiménez, Mario Rodríguez-Monroy

The fungus Trichoderma asperellum is a biological control agent against phytopathogenic fungi; however, the impact of substrate moisture on its metabolic and antagonistic responses during solid-state fermentation has been little studied, and it was the aim of this research. In addition, the antifungal performance of conidia produced in packed-bed reactors was compared with that of conidia produced in polypropylene bag cultures. Wheat and rice were used as substrates in both systems, and conidial yields were similar in magnitude (1.62 × 10⁸–2.06 × 10⁸ conidia g⁻¹). Still, with wheat, the mycelial growth and conidiation were more uniform, and the protein content and activity of amylases and cellulases were higher than in rice. In the packed-bed reactor with wheat, the highest conidial yield (4.56 × 10⁸ conidia g⁻¹) was obtained at 53 % substrate moisture, while protein content and enzyme activity were negatively affected by increased moisture. Conidia viability (97 %) and germination (91 %) were not affected by moisture content. The T. asperellum conidia produced in the packed-bed reactor inhibited Fusarium spp. mycelial growth by 72–85 %, while those conidia from bag systems inhibited the mycelial growth by 51–78 %. To the best of our knowledge, this is the first study to compare the antagonistic activity of Trichoderma conidia produced in two different configurations. Besides, the results demonstrate a relationship between moisture availability and protein metabolism and enzymatic activity in T. asperellum, under solid-state fermentation. The packed-bed reactor using wheat at 53 % moisture content is a potential alternative for producing T. asperellum conidia.

曲霉木霉是植物病原真菌的生物防治剂；然而，在固体发酵过程中，基质水分对其代谢和拮抗反应的影响研究很少，这是本研究的目的。此外，还比较了填充床反应器产生的分生孢子与聚丙烯袋培养产生的分生孢子的抑菌性能。两个体系均以小麦和水稻为底物，分生孢子产量相近（1.62 × 108 ~ 2.06 × 108孢子g−1）。小麦的菌丝生长和分生更为均匀，淀粉酶和纤维素酶的蛋白质含量和活性均高于水稻。在小麦填充床反应器中，当底物含水量为53%时，分生孢子产量最高，为4.56 × 108个g−1，而增加水分对蛋白质含量和酶活性有负面影响。分生孢子存活率（97%）和萌发率（91%）不受水分含量的影响。填充床反应器中产生的曲霉分生孢子对镰刀菌菌丝生长的抑制作用为72 ~ 85%，而袋式反应器中产生的分生孢子对镰刀菌菌丝生长的抑制作用为51 ~ 78%。据我们所知，这是第一个比较两种不同形态的分生木霉拮抗活性的研究。此外，研究结果还表明，在固态发酵条件下，曲霉水分有效性与蛋白质代谢和酶活性之间存在一定的关系。以含水量为53%的小麦为原料的填料床反应器是生产分生曲霉的一种潜在的替代方法。

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引用次数: 0

Biogenic synthesis and fabrication of starch/PVA/CuO electrospun nanoscaffolds from Sargassum ilicifolium (brown marine macroalgae) extract for anticancer activity on HT-29 and MCF-7 cancer cells 褐藻提取物淀粉/PVA/CuO静电纺丝纳米支架的合成与制备及其对HT-29和MCF-7癌细胞的抗癌作用

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-06 DOI: 10.1016/j.bcab.2026.103923

Rajendraprasad Kunamalla , Mani Panagal , Chella Perumal Palanisamy

This study presents the fabrication of starch/PVA electrospun nanoscaffolds incorporated with ultrasonically synthesized copper oxide nanoparticles (CuO NPs) derived from Sargassum ilicifolium extract, followed by an in-depth assessment of their anticancer potential. The biogenic CuO NPs were characterized using UV–Vis spectroscopy, which confirmed nanoparticle formation through distinct surface plasmon resonance (SPR) bands. Fourier-transform infrared spectroscopy (FTIR) analysis revealed functional group interactions between CuO NPs and the polymeric matrix, while X-ray diffraction (XRD) confirmed the monoclinic crystalline phase of CuO NPs and the semi-crystalline nature of the nanoscaffolds. Scanning electron microscopy (SEM) imaging demonstrated a well-organized, porous fibrous structure in the scaffolds, and Thermogravimetric analysis (TGA) indicated thermal stability up to 374 °C. Dynamic light scattering (DLS) revealed moderate colloidal stability, with an average particle size of 108 nm and a zeta potential of −18.3 mV. The anticancer efficacy of the algal extract, CuO NPs, and nanoscaffolds was evaluated against HT-29 colon cancer and MCF-7 breast cancer cells via the MTT assay. The nanoscaffolds exhibited superior cytotoxicity, with IC₅₀ values below 12.5 μg/mL for both cell lines, outperforming standalone CuO NPs (IC₅₀: 12.5–25 μg/mL) and the algal extract (IC₅₀: 25–50 μg/mL). These findings suggest that the starch/PVA/CuO-NPs nanoscaffolds enhance anticancer activity, likely due to improved cellular uptake or sustained release mechanisms. This study highlights the potential of these nanoscaffolds as a promising platform for cancer therapy, warranting further investigation into their mechanistic pathways and in vivo applications.

本研究介绍了淀粉/聚乙烯醇静电纺丝纳米支架的制备，并结合超声合成的氧化铜纳米颗粒（CuO NPs），这些纳米颗粒来自马尾藻提取物，随后深入评估了它们的抗癌潜力。利用紫外可见光谱对生物源CuO纳米粒子进行了表征，通过不同的表面等离子体共振（SPR）波段证实了纳米粒子的形成。傅里叶红外光谱（FTIR）分析揭示了CuO NPs与聚合物基体之间的官能团相互作用，x射线衍射（XRD）证实了CuO NPs的单斜晶相和纳米支架的半晶性质。扫描电镜（SEM）成像显示支架具有组织良好的多孔纤维结构，热重分析（TGA）表明支架的热稳定性高达374°C。动态光散射（DLS）显示出中等的胶体稳定性，平均粒径为108 nm， zeta电位为−18.3 mV。通过MTT法评估海藻提取物、CuO NPs和纳米支架对HT-29结肠癌细胞和MCF-7乳腺癌细胞的抗癌作用。纳米支架具有优异的细胞毒性，两种细胞系的IC50值均低于12.5 μg/mL，优于单独的CuO NPs （IC50: 12.5 - 25 μg/mL）和藻类提取物（IC50: 25-50 μg/mL）。这些发现表明淀粉/PVA/CuO-NPs纳米支架增强抗癌活性，可能是由于细胞摄取或持续释放机制的改善。这项研究强调了这些纳米支架作为一种有前途的癌症治疗平台的潜力，值得进一步研究它们的机制途径和体内应用。

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引用次数: 0

Production, chemical characterization and bioactive properties of cell-bound and extracellular biosurfactants from a newly isolated, potential probiotic Lactobacillus strain grown on dairy wastes 一种新分离的潜在益生菌乳酸菌菌株的细胞结合和细胞外生物表面活性剂的生产、化学特性和生物活性

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-05 DOI: 10.1016/j.bcab.2026.103922

Dimitra Alimpoumpa, Maria Alexandri, Harris Papapostolou, Nikolaos Kopsahelis

The use of health-promoting microorganisms for biosurfactant (BS) production could expand their applications, especially in novel food formulations. This study investigated fourteen newly isolated lactic acid bacterial strains for their ability to produce cell-bound (CB) and extracellular (EC) BS using deproteinized cheese whey (CW) as substrate. Among the tested isolates, the potential probiotic strain Lactiplantibacillus plantarum F254 showed the most promising results in terms of substrate utilization and BS production. In batch bioreactor fermentations with CW as the sole nutrient source, the strain was able to produce 0.25 g/L of cell-bound BS (CB-BS) and 0.51 g/L of extracellular BS (EC-BS). Critical micelle concentration (CMC) was lower for CB-BS (0.20 g/L) compared to the EC-BS (1.07 g/L). ATR-FTIR and chromatographic analyses point to a glycolipopeptide nature for both BS types composed mainly of glucose, galactose, and palmitic acid; however, amino acid profiles differed: CB-BS contained predominantly arginine and glutamic acid, while EC-BS were mainly composed of phenylalanine, isoleucine, and proline. Both BS types were also able to inhibit several pathogenic microorganisms, while EC-BS could completely suppress the growth of Staphylococcus aureus. This is the first report describing simultaneous production and characterization of both CB and EC-BS by L. plantarum F254. The ability to obtain bioactive BS from a potential probiotic strain using dairy waste as substrate demonstrates clear bioprocess and industrial relevance, providing a sustainable route for valorizing CW while generating natural compounds suitable for clean-label food formulations or other high-value applications.

利用有益健康的微生物生产生物表面活性剂（BS）可以扩大其在新型食品配方中的应用。本研究研究了14株新分离的乳酸菌菌株以脱蛋白奶酪乳清（CW）为底物产生细胞结合（CB）和细胞外（EC） BS的能力。在所测试的菌株中，潜在的益生菌菌株植物乳杆菌F254在底物利用和BS生产方面表现出最有希望的结果。在以CW为唯一营养源的间歇生物反应器发酵中，菌株能产生0.25 g/L的细胞结合BS （CB-BS）和0.51 g/L的细胞外BS （EC-BS）。CB-BS的临界胶束浓度（CMC）为0.20 g/L，低于EC-BS的1.07 g/L。ATR-FTIR和色谱分析表明，这两种BS类型主要由葡萄糖、半乳糖和棕榈酸组成的糖多糖肽性质；然而，氨基酸分布不同：CB-BS主要含有精氨酸和谷氨酸，而EC-BS主要由苯丙氨酸、异亮氨酸和脯氨酸组成。两种BS均能抑制多种病原微生物，其中EC-BS能完全抑制金黄色葡萄球菌的生长。这是首次报道L. plantarum F254同时生产CB和EC-BS并进行鉴定。利用乳制品废物作为底物从潜在的益生菌菌株中获得生物活性BS的能力表明了明确的生物过程和工业相关性，为CW的增值提供了可持续的途径，同时产生适合清洁标签食品配方或其他高价值应用的天然化合物。

{"title":"Production, chemical characterization and bioactive properties of cell-bound and extracellular biosurfactants from a newly isolated, potential probiotic Lactobacillus strain grown on dairy wastes","authors":"Dimitra Alimpoumpa, Maria Alexandri, Harris Papapostolou, Nikolaos Kopsahelis","doi":"10.1016/j.bcab.2026.103922","DOIUrl":"10.1016/j.bcab.2026.103922","url":null,"abstract":"<div><div>The use of health-promoting microorganisms for biosurfactant (BS) production could expand their applications, especially in novel food formulations. This study investigated fourteen newly isolated lactic acid bacterial strains for their ability to produce cell-bound (CB) and extracellular (EC) BS using deproteinized cheese whey (CW) as substrate. Among the tested isolates, the potential probiotic strain <em>Lactiplantibacillus plantarum</em> F254 showed the most promising results in terms of substrate utilization and BS production. In batch bioreactor fermentations with CW as the sole nutrient source, the strain was able to produce 0.25 g/L of cell-bound BS (CB-BS) and 0.51 g/L of extracellular BS (EC-BS). Critical micelle concentration (CMC) was lower for CB-BS (0.20 g/L) compared to the EC-BS (1.07 g/L). ATR-FTIR and chromatographic analyses point to a glycolipopeptide nature for both BS types composed mainly of glucose, galactose, and palmitic acid; however, amino acid profiles differed: CB-BS contained predominantly arginine and glutamic acid, while EC-BS were mainly composed of phenylalanine, isoleucine, and proline. Both BS types were also able to inhibit several pathogenic microorganisms, while EC-BS could completely suppress the growth of <em>Staphylococcus aureus</em>. This is the first report describing simultaneous production and characterization of both CB and EC-BS by <em>L. plantarum</em> F254. The ability to obtain bioactive BS from a potential probiotic strain using dairy waste as substrate demonstrates clear bioprocess and industrial relevance, providing a sustainable route for valorizing CW while generating natural compounds suitable for clean-label food formulations or other high-value applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103922"},"PeriodicalIF":3.8,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microplastic exposure and its impact on the growth and physiological functions of Cicer arietinum 微塑性暴露及其对黄鳝生长和生理功能的影响

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-03 DOI: 10.1016/j.bcab.2026.103920

Garima Kaushik, P. Manuraj, Mohd Ashraf Dar, Preksha Palsania, Shalni Satya, Ommer Ahad Bhat, Shailesh Kumar Patidar

The ubiquity of plastic waste pollution, a hallmark of the Anthropocene, leads to microplastic (MP) contamination in soil, posing risks to plant health by significantly impairing overall growth and reduced vegetative development. This study explores the effects of varying polyethylene terephthalate (PET) MP concentrations (1 %, 2 %, and 4 % w/w) on the growth and cellular function of Cicer arietinum. Results indicate a dose-dependent decline in seed germination, seedling vigor index, and cumulative germination energy, suggesting compromised early plant development. A significant reduction in biomass was observed at higher MP concentrations, with plant dry weight decreasing from 23.91 mg in the control to 11.24 mg at 4 % MP exposure. In contrast, leaf area and width remained less affected, implying selective growth responses under stress. MP exposure was associated with signs of impaired photosynthesis, as evidenced by a sharp reduction in total chlorophyll content from 64.5 mg/L in the control to 17.6 mg/L in the 1 % MP treatment. Cellular health markers also declined; dehydrogenase activity was diminished in all MP treatments, and exposure was linked to severe membrane injury, with relative cell injury increasing from 65.2 % in the control to 95.1 % in the 4 % MP treatment. Notably, ascorbic acid levels increased from 4.66 μg/g to 6.59 μg/g with higher MP concentrations, suggesting an active defensive stress response. This study documents a suite of negative physiological effects associated with PET MP exposure, providing a quantitative basis for further research into MP toxicity and crop resilience.

塑料废物污染是人类世的一个标志，无处不在，导致土壤中的微塑料污染，通过严重损害整体生长和减少植物发育，对植物健康构成风险。本研究探讨了不同的聚对苯二甲酸乙二醇酯（PET） MP浓度（1%，2%和4% w/w）对西芹（Cicer arietinum）生长和细胞功能的影响。结果表明，种子萌发率、幼苗活力指数和累积萌发能呈剂量依赖性下降，表明植物早期发育受到损害。较高的MP浓度显著降低了生物量，植物干重从对照的23.91 mg下降到4% MP浓度下的11.24 mg。相反，叶面积和叶宽受影响较小，表明胁迫下的选择性生长响应。MP暴露与光合作用受损的迹象有关，总叶绿素含量从对照的64.5 mg/L急剧下降到1% MP处理的17.6 mg/L。细胞健康指标也有所下降；脱氢酶活性在所有MP处理中都降低，暴露与严重的膜损伤有关，相对细胞损伤从对照组的65.2%增加到4% MP处理的95.1%。值得注意的是，随着MP浓度的升高，抗坏血酸水平从4.66 μg/g增加到6.59 μg/g，表明抗坏血酸是一种主动的防御性应激反应。本研究记录了与PET多聚物暴露相关的一系列负面生理效应，为进一步研究多聚物毒性和作物抗逆性提供了定量基础。

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引用次数: 0

Chitosan oligomers and triacontanol trigger growth, photosynthesis, enzyme activity, and essential oil content in field-grown lemongrass 壳聚糖低聚物和三康醇会影响田间种植柠檬草的生长、光合作用、酶活性和精油含量

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-01 DOI: 10.1016/j.bcab.2025.103911

M. Masroor A. Khan , Shakir Bhat , Mohammad Mukarram , Peter Fleischer Jr. , Jamin Ali , Chen Ri Zhao , Qiyun Li

Lemongrass is an industrial crop primarily cultivated for its oil, as lemongrass essential oil (LEO) contains several bioactive constituents with pharmaceutical and cosmetic potential. Although recent decades have witnessed an increase in LEO demand, its production remains relatively low. The present study hypothesised that chitosan oligomers (COS) and triacontanol (TRIA) would increase LEO productivity. For this, foliar sprays of COS and TRIA were applied to the field-grown lemongrass plants. While all the COS and TRIA treatments elicited lemongrass growth and development, the optimum effect was observed with the simultaneous application of COS (80 mg L⁻¹) and TRIA (10⁻⁶ M). This treatment increased plant height, tiller numbers, fresh weight, and dry weight by 32.4 %, 29.2 %, 27.9 %, and 68.3 % over the control. Similar upregulation was observed in chlorophyll (19.1 %), carotenoids (18.2 %), and chlorophyll fluorescence (38.9 %), as well as in carbonic anhydrase (20.5 %) and nitrate reductase (18.3 %) activities. The same treatment also increased the total essential oil content (53.7 %), essential oil yield (95.7 %), citral content (41.67 %), and citral yield (107.7 %) compared to the control. Overall, the combined application of COS (80 mg L⁻¹) and TRIA (10⁻⁶ M) proved the most effective for eliciting general lemongrass physiology. This opens new possibilities for their biotechnological application in other related cash crops. Nonetheless, prospective studies could investigate the molecular mechanism of action and whether these elicitors influence the gene expression of metabolic pathways. This would lead to more targeted applications.

柠檬草是一种主要因其精油而种植的工业作物，因为柠檬草精油（LEO）含有几种具有制药和化妆品潜力的生物活性成分。尽管近几十年来LEO需求有所增加，但其产量仍然相对较低。本研究假设壳聚糖低聚物（COS）和三康醇（TRIA）可以提高LEO的产率。为此，在田间种植的柠檬草上喷施COS和TRIA。COS和TRIA处理均能促进柠檬草生长发育，其中COS （80 mg L−1）和TRIA（10−6 M）同时施用效果最佳。该处理使植株高度、分蘖数、鲜重和干重分别比对照提高了32.4%、29.2%、27.9%和68.3%。叶绿素（19.1%）、类胡萝卜素（18.2%）、叶绿素荧光（38.9%）以及碳酸酐酶（20.5%）和硝酸盐还原酶（18.3%）活性均出现了类似的上调。与对照相比，同样的处理也增加了总精油含量（53.7%）、精油得率（95.7%）、柠檬醛含量（41.67%）和柠檬醛得率（107.7%）。总体而言，COS （80 mg L−1）和TRIA（10−6 M）的联合施用对柠檬草的一般生理反应最有效。这为它们在其他相关经济作物中的生物技术应用开辟了新的可能性。尽管如此，前瞻性研究可以探讨其作用的分子机制以及这些激发子是否影响代谢途径的基因表达。这将导致更有针对性的应用程序。

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引用次数: 0

Phytochemical profiles, antioxidant, antibacterial, and MCF-7 breast cancer cell cytotoxic activities of Javanese Myristica fragrans mace essential oil with an in-silico study to evaluate its mechanisms of action 用硅片研究了爪哇肉豆酱精油的植物化学特征、抗氧化、抗菌和MCF-7乳腺癌细胞毒活性，以评价其作用机制

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-01 DOI: 10.1016/j.bcab.2025.103793

Tia Okselni, Grace Serepina Saragih, Ariyanti Saputri, Sukirno, Faris Hermawan, Megawati, Marissa Angelina, Rizna Triana Dewi, Sofa Fajriah, Abdi Wira Septama

Myristica fragrans is a native Indonesian plant with a particular aroma. Indonesia has become the biggest supplier of nutmeg and mace from this plant. Besides culinary applications, mace has also been recognized for its potential to treat dysentery, leading to further scientific evaluation. This study evaluated the antioxidant and antibacterial activities of M. fragrans mace essential oil (MFMEO). The predominant constituents of MFMEO were α-pinene (16.32 %), sabinene (11.13 %), terpinene-4-ol (10.85 %), D-limonene (8.58 %), and β-pinene (8.57 %). MFMEO revealed good antioxidant activity, especially with ABTS assay with 79.67 % inhibition at a 0.1 % (v/v) concentration. Antibacterial evaluation of MFMEO against selected foodborne pathogens of Escherichia coli, Salmonella typhi, and Staphylococcus aureus exhibited potential activity with inhibition zone diameters of 15.67, 8.67, 14.33 mm (disk diffusion method), and the minimum inhibitory concentrations (MICs) of 1.56, 25, and 25 %, respectively. Further evaluation of MFMEO showed a significant decrease in biofilm formations (P < 0.05) and a synergistic effect with colistin (FICI; fractional inhibition concentration index of 0.3125) toward E. coli bacteria. The MFMEO-colistin combination reduced 4-fold MFMEO MIC and 16-fold colistin MIC. The 10 % MFMEO also significantly reduced MCF-7 breast cancer cell viability to 7.46 ± 0.46 %. Molecular docking studies showed that D-limonene had the lowest binding energy for antigen 43 (−4.21 kcal/mol) and OMP-F (−4.38 kcal/mol), sabinene for OMP-X (−4.10 kcal/mol), and β-pinene for estrogen receptor α (−5.49 kcal/mol). These findings suggest that MFMEO has potential as an antioxidant and a therapeutic option for treating foodborne infections and degenerative diseases.

香豆蔻是一种印尼本土植物，有一种特殊的香气。印尼已成为该工厂生产的肉豆蔻和肉豆蔻的最大供应国。除了烹饪应用外，梅斯梅斯还被认为有治疗痢疾的潜力，因此需要进一步的科学评估。本研究评价了香豆油（MFMEO）的抗氧化和抗菌活性。MFMEO的主要成分为α-蒎烯（16.32%）、sabinene（11.13%）、terpinene-4-ol（10.85%）、d -柠檬烯（8.58%）和β-蒎烯（8.57%）。MFMEO显示出良好的抗氧化活性，特别是在ABTS实验中，0.1% （v/v）浓度的抗氧化率为79.67%。MFMEO对大肠杆菌、伤寒沙门氏菌和金黄色葡萄球菌等食源性致病菌的抑菌效果显示，其抑菌带直径分别为15.67、8.67、14.33 mm（盘扩散法），最小抑菌浓度（mic）分别为1.56、25%和25%。进一步评价表明，MFMEO显著降低了生物膜的形成（P < 0.05），并与粘菌素（FICI，分数抑制浓度指数为0.3125）对大肠杆菌有协同作用。MFMEO-粘菌素组合使MFMEO MIC降低4倍，粘菌素MIC降低16倍。10%的MFMEO也显著降低MCF-7乳腺癌细胞活力至7.46±0.46%。分子对接研究表明，d -柠檬烯对抗原43 （- 4.21 kcal/mol）和OMP-F的结合能最低（- 4.38 kcal/mol）， sabinene对OMP-X的结合能最低（- 4.10 kcal/mol）， β-蒎烯对雌激素受体α的结合能最低（- 5.49 kcal/mol）。这些发现表明，MFMEO具有作为抗氧化剂和治疗食源性感染和退行性疾病的治疗选择的潜力。

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引用次数: 0

Harnessing PEG 8000-induced water deficit stress to enhance terpenoid production through morpho-physiological and antioxidant defense system modulation in Artemisia annua under hydroponic conditions 利用PEG 8000诱导的水分亏缺胁迫，通过水培条件下黄花蒿的形态生理和抗氧化防御系统调节来提高萜类化合物的产量

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-01 DOI: 10.1016/j.bcab.2026.103919

Sabitri Kumari , Pajeb Saha , Sneha Singh, Naushad Ansari, Shashi Pandey-Rai

Water deficit stress, induced by PEG 8000, was utilized to investigate its role as an elicitor in Artemisia annua. Seedlings were exposed to 4 %, 8 %, and 12 % PEG treatments in half-strength Hoagland solution for 7 days, with 8 % PEG at 5 days determined as optimum for subsequent investigations. Under extreme stress (12 % PEG), a notable decrease in shoot length (55.4 %) and shoot fresh weight (65 %) was recorded by day 7, whereas moderate stress (8 % PEG) maintained structural integrity. Physiological responses indicated a dose-dependent drop in stomatal conductance 58 % and CO₂ assimilation rates 38 % under severe stress, whereas moderate stress resulted in tolerable decreases. Biochemical research demonstrated a 3.3-fold increase in proline and an 8.7-fold elevation in glycine-betaine under 8 % PEG by day 7, underscoring osmo-protectant build-up. The activities of antioxidant enzymes reached their zenith with 8 % PEG treatment, with SOD, CAT, POD, and APX activities escalating by 3.3, 2.6, 3.0, and 2.7 folds, respectively, alleviating oxidative stress. Raman spectroscopy and HRMS profiling revealed metabolic alterations under 8 % PEG-induced stress, with artemisinin levels increasing by 1.6-fold and terpenes jumping from 17.9 % to 25 % in relative abundance. Further heatmap analysis revealed that phenolics, flavonoids, and terpenes are metabolites that are induced under stress. HRMS analysis indicated that 48.2 % of metabolites under stress were distinct, comprising stress-reducing terpenes and antioxidants. These findings validate 8 % PEG as a potent elicitor for metabolic reprogramming in A. annua, augmenting secondary metabolite production while preserving resistance, with ramifications for the enhancement of bioactive chemical synthesis in medicinal plants.

利用PEG 8000诱导的水分亏缺胁迫，研究了其在黄花蒿（Artemisia annua）中的激发子作用。幼苗在半强度Hoagland溶液中暴露于4%，8%和12% PEG处理7天，其中5天的8% PEG被确定为后续研究的最佳处理。在极端胁迫（12% PEG）下，第7天茎长（55.4%）和鲜重（65%）显著减少，而中等胁迫（8% PEG）保持结构完整。生理反应表明，在重度胁迫下，气孔导度和CO2同化率分别下降58%和38%，而中度胁迫导致可耐受的下降。生化研究表明，在8% PEG条件下，第7天脯氨酸增加3.3倍，甘氨酸-甜菜碱增加8.7倍，强调渗透保护剂的积累。8% PEG处理后，抗氧化酶活性达到峰值，SOD、CAT、POD和APX活性分别提高3.3倍、2.6倍、3.0倍和2.7倍，氧化应激得到缓解。拉曼光谱和HRMS分析显示，在8% peg诱导的应激下，代谢发生了变化，青蒿素水平增加了1.6倍，萜烯的相对丰度从17.9%跃升至25%。进一步的热图分析表明，酚类物质、类黄酮和萜烯是应激诱导的代谢产物。HRMS分析表明，在应激条件下，48.2%的代谢产物是不同的，包括抗应激萜类和抗氧化剂。这些发现证实了8% PEG是一种有效的引发子，可以在保持抗性的同时增加次生代谢物的产生，从而增强药用植物的生物活性化学合成。

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引用次数: 0

Harnessing multivariate statistical models for enhanced L-asparaginase production from Fusarium solani ITCC 9504 and its biotechnological applications 茄枯菌ITCC 9504产l -天冬酰胺酶的多元统计模型及其生物技术应用

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-01 DOI: 10.1016/j.bcab.2026.103921

Shivangi Mudaliar , Lakshana G. Nair , Venkatesh Chaturvedi , Pradeep Verma

L-asparaginase (L-ASNase) production was optimized and performed using the fungal strain Fusarium solani ITCC 9504 (F. solani ITCC 9504) via a multivariate statistical approach. It demonstrated significant production potential in a modified Czepak Dox (CD) medium under submerged cultivation conditions. In the present study, Response Surface Methodology (RSM) was employed based on the optimum conditions revealed by the one-factor-at-a-time (OFAT) approach. Two models were designed to study the effect of L-asparagine (Model I) and peptone (Model II) on common variables, including pH, temperature, and glycerol concentration. In Model I, a high L-ASNase activity of 60.41 U/mL (constant peptone concentration) was achieved under optimized conditions of pH 5.2, 27 °C, 6.0 g/L L-ASN, and 3.0 g/L glycerol. In model II, the L-ASNase activity was further enhanced to 65.32 U/mL (constant L-ASN concentration) with 5.0 g/L peptone, 3.0 g/L glycerol, pH 5.2, and at a temperature of 27 °C. L-ASNase was also evaluated for biotechnological applications, wherein an acrylamide reduction in fried potato slices was observed to be dose-dependent on L-ASNase, with a maximum reduction of 51 % observed at the high concentration of L-ASNase. The cytotoxicity assay of the L-ASNase also identified activity against A549 (Lung adenocarcinoma) cell lines. The biotechnological applications of L-ASNase, such as acrylamide reduction and cytotoxicity, also reveal its potential for use in the food and pharmaceutical industries.

采用多元统计方法对茄枯萎菌ITCC 9504 （F. solani ITCC 9504）生产l -天冬酰胺酶（L-ASNase）进行了优化。在潜水培养条件下，在改良的Czepak Dox （CD）培养基中显示出显著的生产潜力。在此基础上，采用响应面法（Response Surface Methodology， RSM）对单因素法（OFAT）的优化条件进行了研究。设计了两个模型来研究l -天冬酰胺（模型I）和蛋白胨（模型II）对pH、温度和甘油浓度等常见变量的影响。在模型I中，在pH 5.2、27℃、6.0 g/L L- asn、3.0 g/L甘油的优化条件下，L- asnase活性达到60.41 U/mL（恒定蛋白胨浓度）。在模型II中，当蛋白胨5.0 g/L，甘油3.0 g/L, pH 5.2，温度27℃时，L- asnase活性进一步增强至65.32 U/mL（恒定L- asn浓度）。L-ASNase也被评估为生物技术应用，其中，在油炸土豆片中丙烯酰胺的减少被观察到与L-ASNase的剂量依赖，在高浓度的L-ASNase下观察到的最大减少量为51%。L-ASNase对A549（肺腺癌）细胞株的细胞毒性试验也证实了其活性。L-ASNase的生物技术应用，如丙烯酰胺还原和细胞毒性，也显示了它在食品和制药工业中的应用潜力。

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引用次数: 0

Antifungal properties of plant polyphenols-copper complex used as a wood preservative in rubber wood impregnation 植物多酚-铜配合物在橡胶木材浸渍中的防腐性能

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2026-01-01 DOI: 10.1016/j.bcab.2026.103918

Hong Yun , Yuyou Liu , Xiaoyan Wang , Limin Wang

To enhance the leaching resistance of copper-based preservatives, we developed an innovative wood preservative by complexing plant polyphenols with copper, achieving both superior antifungal efficacy and enhanced leaching resistance. The results showed that the grape-seed polyphenols complex copper preservative prepared at pH 2 had the best dispersion and antifungal properties, and the inhibition zones for white-rot fungus and brown-rot fungus were 50.77 mm and 43.40 mm, respectively. The retention of copper in the preservative-treated wood impregnated by one-step and two-step impregnation method was 10.02 % and 8.50 %, respectively. The Fourier transform infrared and scanning electron microscopy analyses showed the phenolic hydroxyl group of the preservative and the hydroxyl group of the wood cells cross-linked to form hydrogen bonds. After 14 days of anti-loss treatment, the fixation rate of copper in the preservative wood obtained by the two impregnation methods reached 96.49 % and 99.72 %, respectively, neither of the two types of preservative-treated wood was infected with mold. The antifungal properties and in-situ immobilization of the plant polyphenol-copper composite preservative prepared in this study have been verified, and this plant-derived composite preservative has great development potential.

为了提高铜基防腐剂的抗浸出性，我们开发了一种创新的木材防腐剂，通过植物多酚与铜络合，既具有优异的抗真菌效果，又增强了抗浸出性。结果表明，pH为2条件下制备的葡萄籽多酚络合铜保鲜剂对白腐菌和褐腐菌的抑制区分别为50.77 mm和43.40 mm，具有最佳的分散和抑菌性能。一步浸渍法和两步浸渍法处理过的木材中铜的保留率分别为10.02%和8.50%。傅里叶变换红外和扫描电镜分析表明，防腐剂的酚羟基与木细胞的羟基交联形成氢键。防腐处理14 d后，两种浸渍方法获得的防腐木材中铜的固结率分别达到96.49%和99.72%，两种防腐木材均未发生霉菌感染。本研究制备的植物多酚-铜复合防腐剂的抗真菌性能和原位固定化性能均已得到验证，该植物源性复合防腐剂具有很大的开发潜力。

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引用次数: 0