Biocatalysis and agricultural biotechnology最新文献_第3页

β-Glucosidase from Lacticaseibacillus casei TISTR 1463: Biochemical characterization and application on biotransformation of pigmented leaf extract from Oryza sativa L. (Luem Pua glutinous rice)

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103491

Sirinthip Jaijoi , Tanyawat Kaewsalud , Kamon Yakul , Sugunya Mahatheeranont , Woraprapa Sriyotai , Sarana Rose Sommano , Pornchai Rachtanapun , Noppol Leksawasdi , Masanori Watanabe , Thanongsak Chaiyaso

This study aimed to biochemically characterize a purified β-glucosidase from a probiotic Lacticaseibacillus casei TISTR 1463 and to apply the enzyme for improving antioxidant activities of Luem Pua (Oryza sativa L.) pigmented rice leaf extract (PRLE-LP). The effect of PRLE-LP supplementation in de Man, Rogosa, and Sharpe (MRS) medium, inoculum size, initial pH, and temperature were investigated using one-factor-at-a-time (OFAT) approach. Under optimal conditions, the maximal β-glucosidase activity of 38.43 ± 0.01 U/g _DCW was achieved by cultivation strain TISTR 1463 in MRS-PRLE-LP medium (20:80% (v/v)), 10% (v/v) inoculum size, initial pH 3.5 at 30 °C for 36 h. Afterward, β-glucosidase was purified to 7.5-fold with 37% recovery yield and a molecular weight (MW) of 75 kDa. This purified enzyme had an optimal pH and temperature of pH 4.5 and 35 °C. It was stable under pH of 3.0–5.0 and temperature of 30–35 °C and showed the highest specific activity toward 4-nitrophenyl β-D-glucopyranoside (p-NPG) with the K_m and V_max of 1.31 mg/mL and of 0.06 μmol/min/mg. The enhancement of antioxidant activities of PRLE-LP by purified β-glucosidase from strain TISTR 1463 and commercial enzyme was also studied. An in-house β-glucosidase displayed superior antioxidant activities over the commercial enzyme from Aspergillus niger. In addition, LC-QTOF-MS analysis confirmed that β-glucosidase efficiently converted glycone into aglycone, resulting in enhanced antioxidant activities. The potential for producing antioxidant-rich substances from anthocyanin-containing alternative crops with applications in food and pharmaceutical industries that are both health and eco-friendly is thus addressed.

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引用次数: 0

Biofertilizers as an eco-friendly approach to combat drought stress in plants

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103510

Zhenqi Liao , Hatem Boubakri , Beibei Chen , Muhammad Farooq , Zhenlin Lai , Hongtai Kou , Junliang Fan

Drought is a severe abiotic stress adversely affecting agricultural activity worldwide. Biofertilizers have emerged as an environmentally-safe tool to combat drought adverse effects in plants. Biofertilizers like endophytic fungi (EF), arbuscular mycorrhizal fungi (AMF), plant growth-promoting bacteria (PGPB) and natural compounds have enormous potential to mitigate drought negative effects in plants. Microbials like PGPB and AMF improve physical, chemical and biological features of soils by promoting nutrient uptake and water flow. However, natural compounds provide drought stress tolerance by inducing morphological and structure changes, water status adjustment, stomatal regulation, osmotic regulation, ion balance and pH regulation, reactive oxygen metabolism, antioxidant defense system, photosynthetic system, plant hormones, signal transduction, and gene expression regulation. This review provides valuable background knowledge on various biofertilizers that have proven effective against drought and serves as theoretical basis for conducting future research under field conditions to validate their practical use.

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引用次数: 0

Optimization of medium formulations for biomass vaccine production of gdhA derivative Pasteurella multocida B:2 using statistical experimental design

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103504

Siti Nur Hazwani Oslan , Jiun Shen Loo , Rosfarizan Mohamad , Siti Khairani Bejo , Mohd Zamri Saad

The glutamate dehydrogenase (gdhA) gene of a pathogenic Pasteurella multocida B:2 was successfully inactivated to create an attenuated strain as a vaccine candidate against hemorrhagic septicemia (HS) in ruminants. This study presents a novel approach to optimizing the medium formulation for high-throughput mass production of a gdhA-inactivated P. multocida B:2, which is critical for scaling up vaccine production. Using response surface methodology (RSM) with a central composite design (CCD), we systematically investigated the effects of various medium components on biomass yield. Yeast extract, glucose, sodium chloride, and sodium phosphate were identified as critical factors, with yeast extract demonstrating a significant enhancement in biomass production, yielding 2.03 ± 0.15 mg/mL, compared to traditional peptone (1.30 ± 0.26 mg/mL) and inorganic nitrogen sources like ammonium chloride and ammonium sulfate (<1.0 mg/mL). Among carbon sources, glucose paired with yeast extract produced the highest biomass, while sucrose, white sugar, and soluble starch had minimal effects. Optimization through CCD identified the ideal concentrations of yeast extract, glucose, NaCl, and NaH₂PO₄ as 15.64 g/L, 1.91 g/L, 3.06 g/L, and 2.48 g/L, respectively, resulting in a 35% increase in biomass yield to 3.10 mg/mL. Yeast extract was the key driver of growth, with optimal concentrations between 5 and 20 g/L, while excess glucose, NaCl, and NaH₂PO₄ inhibited growth. Statistical analysis revealed that the quadratic polynomial model fit the data well (R² = 0.8440, model F-value = 5.80, p < 0.05). The novelty of this study lies in the optimization of a medium that significantly improves biomass production compared to conventional formulations, providing a cost-effective and efficient solution for scaling up the production of attenuated P. multocida B:2. This optimized medium holds promise for advancing vaccine development against HS.

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引用次数: 0

Comparative analysis of Polyphenolic Compound Production from Rubus adenotrichos Schltdl. in a stirred tank bioreactor using two different impellers

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103516

Alexander Schmidt-Durán , Laura A. Calvo-Castro , Oscar Acosta-Montoya , Mario Rodríguez-Monroy

Rubus adenotrichos Schltdl. has a high content of polyphenolic compounds with antioxidant properties; however, obtaining these from the fruits presents limitations because it depends on the plant physiology, developmental stage, and environmental conditions. Cell cultures in a stirred tank bioreactor (STR) offer an alternative for obtaining plant secondary metabolites. The objective of this study was to evaluate the effect of two impellers with different mixing patterns, a marine impeller, and a Rushton turbine impeller, on the culture of R. adenotrichos cells and the production of gallic acid, vanillin and ellagic acid, using an STR. It was observed that the marine impeller enhanced cell growth, reaching 2.320 g L⁻¹ of dry biomass, but did not promote the accumulation of polyphenolic compounds, producing 0.528 mg L⁻¹. Conversely, with the Rushton turbine, cell growth was limited (1.680 g L⁻¹ dry biomass), but the production of the target metabolites was favored, producing a total of 1.043 mg L⁻¹. The decrease in biomass and the increase in specialized metabolite content may be associated with smaller eddy sizes produced by the Rushton turbine, leading to the activation of lethal and sublethal mechanisms in plant cells due to hydrodynamic stress. Additionally, thermal concentration treatments differentially affected each analyzed metabolite, with gallic acid being the least affected compound when the concentration temperature was increased. The results establish the basis for the bioprocess advancement of R. adenotrichos Schltdl., focused on developing a sustainable strategy for natural resource management and as a source of phenolic compounds on a large scale.

{"title":"Comparative analysis of Polyphenolic Compound Production from Rubus adenotrichos Schltdl. in a stirred tank bioreactor using two different impellers","authors":"Alexander Schmidt-Durán , Laura A. Calvo-Castro , Oscar Acosta-Montoya , Mario Rodríguez-Monroy","doi":"10.1016/j.bcab.2025.103516","DOIUrl":"10.1016/j.bcab.2025.103516","url":null,"abstract":"<div><div><em>Rubus adenotrichos</em> Schltdl. has a high content of polyphenolic compounds with antioxidant properties; however, obtaining these from the fruits presents limitations because it depends on the plant physiology, developmental stage, and environmental conditions. Cell cultures in a stirred tank bioreactor (STR) offer an alternative for obtaining plant secondary metabolites. The objective of this study was to evaluate the effect of two impellers with different mixing patterns, a marine impeller, and a Rushton turbine impeller, on the culture of <em>R. adenotrichos</em> cells and the production of gallic acid, vanillin and ellagic acid, using an STR. It was observed that the marine impeller enhanced cell growth, reaching 2.320 g L<sup>−1</sup> of dry biomass, but did not promote the accumulation of polyphenolic compounds, producing 0.528 mg L<sup>−1</sup>. Conversely, with the Rushton turbine, cell growth was limited (1.680 g L<sup>−1</sup> dry biomass), but the production of the target metabolites was favored, producing a total of 1.043 mg L<sup>−1</sup>. The decrease in biomass and the increase in specialized metabolite content may be associated with smaller eddy sizes produced by the Rushton turbine, leading to the activation of lethal and sublethal mechanisms in plant cells due to hydrodynamic stress. Additionally, thermal concentration treatments differentially affected each analyzed metabolite, with gallic acid being the least affected compound when the concentration temperature was increased. The results establish the basis for the bioprocess advancement of <em>R. adenotrichos</em> Schltdl., focused on developing a sustainable strategy for natural resource management and as a source of phenolic compounds on a large scale.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103516"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoremediation for sustainable remediation of environmental pollutants

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103526

Amlan Roy , Nirmali Gogoi , Fasih Ullah Haider , Muhammad Farooq

Mycoremediation is an eco-friendly and sustainable biotechnology approach that helps detoxify the environment by removing heavy metals, pesticides, and organic pollutants, particularly in agricultural settings. This method enhances soil health while supporting crop productivity. Despite the identification of approximately 150,000 fungal species, only a limited number have been explored for mycoremediation. While wood-decaying fungi are well-known for their ligninolytic enzymes, other fungal communities—such as endophytic, soil, and aquatic fungi beyond the saprophytic category—have received comparatively less attention. This review offers a comprehensive overview of the mechanisms employed by diverse ecological groups of fungi for contaminant remediation, including biotransformation, bioabsorption, and immobilization. The findings highlight that mycoremediation is an eco-friendly approach to mitigating the toxic effects of heavy metals like cadmium, silver, lead, and mercury, as well as organic pollutants such as phenanthrene, naphthalene, and benzo[a]pyrene. Key factors influencing the efficiency of mycoremediation include temperature, rhizosphere pH, redox potential, and substrate concentration, all of which can either enhance or limit the process. The review also describes on the significance of mycoremediation in agriculture and how it can improve soil health, which may be utilized for increasing crop yield. Despite the promising findings, significant challenges remain in replicating natural conditions within laboratory environments and effectively translating these results into practical applications. Although fungal enzymes offer hope for bioremediation, productivity and stability-related issues limit their efficiency. One approach for increasing enzyme productivity and resistance to pollutants is genetic engineering. Overexpression and mutation can enhance enzyme attributes so that these enzymes may degrade pollutants more efficiently. Furthermore, future work is required to screen strains on a large scale for endophytic fungi and their application in the biodegradation of industrial pollutants.

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引用次数: 0

Enhancing melatonin photostability and controlled release using pH-responsive mesoporous silica nanoparticles for agricultural applications

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103497

Parul Sangwan, Poonam Barala, Vinita Hooda

pH-responsive and thiol-functionalized mesoporous silica nanoparticles (SH-MSNs) encapsulating the phytohormone melatonin were designed to release the hormone in a controlled manner while enhancing its photostability. Melatonin-loaded MSNs were prepared in weight ratios of 1:4, 1:2, 1:1, and 2:1, with the highest loading efficiency of 17.12 μg/mL observed at a 1:1 melatonin/SH-MSNs ratio. Detailed characterization of these MSNs confirmed their functionality: zeta potential and size analyses indicated enhanced stability post-functionalization and loading; FESEM confirmed spherical morphology; EDX and FTIR validated melatonin incorporation and disulfide linkage formation; and DSC and XRD analyses demonstrated the amorphous transformation of melatonin, enhancing dissolution rates. Reduced specific surface area and pore volume, as revealed by N₂ adsorption-desorption studies, confirmed melatonin entrapment. A distinct DSC peak at 118.82 °C reflected the crystalline nature of pure melatonin, contrasting with its amorphous state post-encapsulation. In vitro release studies demonstrated significant pH responsiveness, with 81 % melatonin release at pH 5.5 and 55% release at pH of 6.5 after 48 h due to disulfide bond reduction. Additionally, encapsulation improved melatonin photostability by 64.9% compared to free melatonin. This research suggests that MSNs grafted with redox-responsive gatekeepers could be used in crops to deliver melatonin in a controlled manner. Future research may explore the broader agricultural benefits of these MSNs for controlled release of other phytohormones under various environmental conditions.

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引用次数: 0

Spectral light treatment and exogenous feeding of precursors enhanced the picrosides content in dedifferentiated cell culture of Picrorhiza kurroa

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103501

Mahinder Partap , Amit Kumar , Pawan Kumar , Dinesh Kumar , Ashish R. Warghat

The present investigation evaluates the impact of the light spectrum (red and blue) and precursors treatment on picrosides (P-I, P-II, P-III) and pathway precursors metabolites enrichment in the cell culture of Picrorhiza kurroa. Exogenously, vanillic acid (VA), phenylalanine (PAL), and trans-cinnamic acid (TCNA) precursors were used in 50–150 mg/L concentrations to leaf and rhizome cell suspension of P. kurroa. The maximum content of P-I (0.872 and 0.894 mg/g DW), P-II (0.892 and 0.837 mg/g DW), P-III (0.286 and 0.288 mg/g DW) were quantified in leaf and rhizome cell suspension cultures under red light treatment. The maximum content (mg/g DW) of picrosides (P-I; 9.881, P-II; 3.939, and P-III; 0.884), VA (0.236), and caffeic acid (CFA; 0.20) were quantified in 150 mg/L VA in leaf suspension culture. The maximum content of catalpol (CTP; 5.845 mg/g DW) was quantified in PAL 100 mg/L, while cinnamic acid (CAN; 0.176, 0.179, 0.162 mg/g DW) was quantified in 150 mg/L of VA, PAL, and TCNA. However, in rhizome suspension culture, the maximum content (mg/g DW) of P-I (7.881), P-III (0.964), and VA (0.324) were found in VA 150 mg/L. The results concluded that VA (100–150 mg/L) is most suitable for picrosides enrichment in the cell culture of P. kurroa. The study confirms that red light and vanillic acid (VA) treatment significantly enhanced the picrosides and precursor metabolite in cell cultures. By utilizing the current approach coupled with elicitor treatments and scale-up studies at bioreactor level could facilitate the large-scale production of picrosides.

{"title":"Spectral light treatment and exogenous feeding of precursors enhanced the picrosides content in dedifferentiated cell culture of Picrorhiza kurroa","authors":"Mahinder Partap , Amit Kumar , Pawan Kumar , Dinesh Kumar , Ashish R. Warghat","doi":"10.1016/j.bcab.2025.103501","DOIUrl":"10.1016/j.bcab.2025.103501","url":null,"abstract":"<div><div>The present investigation evaluates the impact of the light spectrum (red and blue) and precursors treatment on picrosides (P-I, P-II, P-III) and pathway precursors metabolites enrichment in the cell culture of <em>Picrorhiza kurroa</em>. Exogenously, vanillic acid (VA), phenylalanine (PAL), and trans-cinnamic acid (TCNA) precursors were used in 50–150 mg/L concentrations to leaf and rhizome cell suspension of <em>P. kurroa</em>. The maximum content of P-I (0.872 and 0.894 mg/g DW), P-II (0.892 and 0.837 mg/g DW), P-III (0.286 and 0.288 mg/g DW) were quantified in leaf and rhizome cell suspension cultures under red light treatment. The maximum content (mg/g DW) of picrosides (P-I; 9.881, P-II; 3.939, and P-III; 0.884), VA (0.236), and caffeic acid (CFA; 0.20) were quantified in 150 mg/L VA in leaf suspension culture. The maximum content of catalpol (CTP; 5.845 mg/g DW) was quantified in PAL 100 mg/L, while cinnamic acid (CAN; 0.176, 0.179, 0.162 mg/g DW) was quantified in 150 mg/L of VA, PAL, and TCNA. However, in rhizome suspension culture, the maximum content (mg/g DW) of P-I (7.881), P-III (0.964), and VA (0.324) were found in VA 150 mg/L. The results concluded that VA (100–150 mg/L) is most suitable for picrosides enrichment in the cell culture of <em>P. kurroa</em>. The study confirms that red light and vanillic acid (VA) treatment significantly enhanced the picrosides and precursor metabolite in cell cultures. By utilizing the current approach coupled with elicitor treatments and scale-up studies at bioreactor level could facilitate the large-scale production of picrosides.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103501"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A DNase from halophilic bacterium Bacillus pacificus targets two notorious biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103515

Sadaf Salim , Vusqa Jadoon , Mugheesa Batool , Irfan Ali , Usman Irshad , Raza Ahmed , Sadaf Qayyum , Awal Noor , Tatheer Alam Naqvi

Biofilm is a sessile microbial community in which microbes are encased in a self-produced matrix called extracellular matrix (EPS), composed of eDNA, proteins and carbohydrates. Microbes in biofilms are several times more resistant to antimicrobial agents and host defense systems, thus are responsible for 60–80 % of human bacterial infections. Therefore, the enzymes that target EPS components can play a vital role against these biofilms. In the present study, an enzyme was isolated from halophilic bacterium Bacillus pacificus strain ROC1 and its antibiofilm potential was checked against the biofilms of Pseudomonas aeruginosa and Staphylococcus aureus. DNase assay confirmed that the enzyme is DNase, which was further confirmed by whole genome sequence analysis that identified the potential DNase gene in ROC1. For the production of DNase Luria Bertani (LB) media was employed and harvested after 24 h of growth. The disruption and inhibition assays confirmed the antibiofilm potential of DNase by inhibiting 80% biofilm biomass of P. aeruginosa and 71% biofilm biomass of S. aureus. It also disrupts the preformed biofilms of both strains, while more disruption was observed in P. aeruginosa (71%) as compared to S. aureus (55 %). Characterization of DNase indicates its robust nature with profound ability to catalyze in wide range of temperature and salt, and the addition of cations effects the enzyme activity.

{"title":"A DNase from halophilic bacterium Bacillus pacificus targets two notorious biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus","authors":"Sadaf Salim , Vusqa Jadoon , Mugheesa Batool , Irfan Ali , Usman Irshad , Raza Ahmed , Sadaf Qayyum , Awal Noor , Tatheer Alam Naqvi","doi":"10.1016/j.bcab.2025.103515","DOIUrl":"10.1016/j.bcab.2025.103515","url":null,"abstract":"<div><div>Biofilm is a sessile microbial community in which microbes are encased in a self-produced matrix called extracellular matrix (EPS), composed of eDNA, proteins and carbohydrates. Microbes in biofilms are several times more resistant to antimicrobial agents and host defense systems, thus are responsible for 60–80 % of human bacterial infections. Therefore, the enzymes that target EPS components can play a vital role against these biofilms. In the present study, an enzyme was isolated from halophilic bacterium <em>Bacillus pacificus</em> strain ROC1 and its antibiofilm potential was checked against the biofilms of <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em>. DNase assay confirmed that the enzyme is DNase, which was further confirmed by whole genome sequence analysis that identified the potential <em>DNase</em> gene in ROC1. For the production of DNase Luria Bertani (LB) media was employed and harvested after 24 h of growth. The disruption and inhibition assays confirmed the antibiofilm potential of DNase by inhibiting 80% biofilm biomass of <em>P. aeruginosa</em> and 71% biofilm biomass of <em>S. aureus</em>. It also disrupts the preformed biofilms of both strains, while more disruption was observed in <em>P. aeruginosa</em> (71%) as compared to <em>S. aureus</em> (55 %). Characterization of DNase indicates its robust nature with profound ability to catalyze in wide range of temperature and salt, and the addition of cations effects the enzyme activity.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103515"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143204539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer potential of Ampelopsis cantoniensis extract: Inducing apoptosis, cell cycle arrest, and targeting cancer stem cells in HepG2 liver cancer cells

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103521

Phu Hung Nguyen , Thi Thanh Huong Le , Dinh Quang Hung Can , Thi Ngoc Thuy Le , Viet Hoang , Hong Phuong Ngo , Thi Thu Huyen Do , Thi Kieu Oanh Nguyen , Van Hung Hoang

Ampelopsis cantoniensis (A. cantoniensis), has a long history of use in traditional medicine in various countries. Despite their extensive use, little is known about their potential anticancer properties. This study aimed to investigate the potential anticancer effects of A. cantoniensis extract (ACE). The inhibitory effect ACE on HepG2 liver cancer cells was assessed through a MTT assay, and the cell cycle and apoptosis were analyzed using flow cytometry. Real-time PCR, immunofluorescence analysis, LC-QToF-MS analysis, and molecular docking were conducted to further characterize its mechanisms of action. ACE inhibited the growth of HepG2 cells, with an IC₅₀ value of 64.2 μg/mL, and also induced apoptosis in liver cancer cells. In addition, ACE induced cell cycle arrest at the G0/G1 phase and significantly altered the expression of several key cell cycle regulatory genes. Importantly, ACE downregulated the characteristics of liver cancer stem cells, including decreased expression of the cancer stem cell marker CD44 and reduced ability to form 3D tumorspheres. Immunofluorescence staining results indicated that ACE suppressed the expression of PCNA, TLR-5, and TLR9 markers while enhancing CAV-1 expression. Furthermore, ACE induced excessive ROS production in cells. A total of 107 different compounds were predicted by high-resolution (LC-QToF-MS) spectral analysis, among which Quercetin-3,7-di-O-beta-D-glucoside and Quercetin-3-arabinoglucoside were predicted to potentially bind to the target proteins of cancer stem cells. ACE is a potential herbal remedy for combating liver cancer.

{"title":"Anticancer potential of Ampelopsis cantoniensis extract: Inducing apoptosis, cell cycle arrest, and targeting cancer stem cells in HepG2 liver cancer cells","authors":"Phu Hung Nguyen , Thi Thanh Huong Le , Dinh Quang Hung Can , Thi Ngoc Thuy Le , Viet Hoang , Hong Phuong Ngo , Thi Thu Huyen Do , Thi Kieu Oanh Nguyen , Van Hung Hoang","doi":"10.1016/j.bcab.2025.103521","DOIUrl":"10.1016/j.bcab.2025.103521","url":null,"abstract":"<div><div><em>Ampelopsis cantoniensis</em> (<em>A. cantoniensis</em>), has a long history of use in traditional medicine in various countries. Despite their extensive use, little is known about their potential anticancer properties. This study aimed to investigate the potential anticancer effects of <em>A. cantoniensis</em> extract (ACE). The inhibitory effect ACE on HepG2 liver cancer cells was assessed through a MTT assay, and the cell cycle and apoptosis were analyzed using flow cytometry. Real-time PCR, immunofluorescence analysis, LC-QToF-MS analysis, and molecular docking were conducted to further characterize its mechanisms of action. ACE inhibited the growth of HepG2 cells, with an IC<sub>50</sub> value of 64.2 μg/mL, and also induced apoptosis in liver cancer cells. In addition, ACE induced cell cycle arrest at the G0/G1 phase and significantly altered the expression of several key cell cycle regulatory genes. Importantly, ACE downregulated the characteristics of liver cancer stem cells, including decreased expression of the cancer stem cell marker CD44 and reduced ability to form 3D tumorspheres. Immunofluorescence staining results indicated that ACE suppressed the expression of PCNA, TLR-5, and TLR9 markers while enhancing CAV-1 expression. Furthermore, ACE induced excessive ROS production in cells. A total of 107 different compounds were predicted by high-resolution (LC-QToF-MS) spectral analysis, among which Quercetin-3,7-di-O-beta-D-glucoside and Quercetin-3-arabinoglucoside were predicted to potentially bind to the target proteins of cancer stem cells. ACE is a potential herbal remedy for combating liver cancer.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103521"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing starch-stabilized biogenic silver nanoparticles for sustainable bacterial blight management in soybean

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology

Pub Date : 2025-02-01 DOI: 10.1016/j.bcab.2025.103509

Federico N. Spagnoletti , Irma N. Torres , Alejandra I. Hernandez , Romina Giacometti

Green nanotechnology is a new strategy that contributes to sustainable agriculture by enhancing crop production, restoring soil quality, and improving pest management control. Green nanotechnology has emerged as a promising strategy to overcome the limitations of conventional chemical synthesis of nanomaterials. In this study, biogenic silver nanoparticles (st-AgNPs) were synthesized in the presence of extracellular metabolites of the fungus Macrophomina phaseolina with further modification of the particle's corona using starch to enhance stability and performance. After confirming the biocidal activity of st-AgNPs, their potential use against bacterial blight caused by Pseudomonas savastanoi in soybean was analyzed. Under controlled conditions, the effects of different NP concentrations were tested on healthy and infected plants. No evidence of phytotoxicity was observed in plants treated with 50–400 μg ml⁻¹ of NPs. A 100 μg ml⁻¹ st-AgNPs dose was the most effective in controlling the disease progression. Subsequent greenhouse experiments showed that infected plants sprayed with st-AgNPs (100 μg ml⁻¹) improved their general status and increased the aerial biomass (36.6%) compared to non-treated plants. Furthermore, spray treatment with NPs partially reversed the negative effect of the infection, showing a 38.9% recovery in the greenness index. Notably, the severity of infection decreased by 78%, with no detectable silver traces in plant leaves. Biochemical analyses revealed that st-AgNPs treatment resulted in a reduction of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX) activities, as well as lipid peroxidation, compared to non-treated infected plants. These findings suggest that the developed st-AgNPs not only effectively control P. savastanoi infection in soybean but also exhibit the potential to serve as a low-impact, environmentally friendly tool for inclusion in plant disease management protocols across various crops.

{"title":"Harnessing starch-stabilized biogenic silver nanoparticles for sustainable bacterial blight management in soybean","authors":"Federico N. Spagnoletti , Irma N. Torres , Alejandra I. Hernandez , Romina Giacometti","doi":"10.1016/j.bcab.2025.103509","DOIUrl":"10.1016/j.bcab.2025.103509","url":null,"abstract":"<div><div>Green nanotechnology is a new strategy that contributes to sustainable agriculture by enhancing crop production, restoring soil quality, and improving pest management control. Green nanotechnology has emerged as a promising strategy to overcome the limitations of conventional chemical synthesis of nanomaterials. In this study, biogenic silver nanoparticles (st-AgNPs) were synthesized in the presence of extracellular metabolites of the fungus <em>Macrophomina phaseolina</em> with further modification of the particle's corona using starch to enhance stability and performance. After confirming the biocidal activity of st-AgNPs, their potential use against bacterial blight caused by <em>Pseudomonas savastanoi</em> in soybean was analyzed. Under controlled conditions, the effects of different NP concentrations were tested on healthy and infected plants. No evidence of phytotoxicity was observed in plants treated with 50–400 μg ml<sup>−1</sup> of NPs. A 100 μg ml<sup>−1</sup> st-AgNPs dose was the most effective in controlling the disease progression. Subsequent greenhouse experiments showed that infected plants sprayed with st-AgNPs (100 μg ml<sup>−1</sup>) improved their general status and increased the aerial biomass (36.6%) compared to non-treated plants. Furthermore, spray treatment with NPs partially reversed the negative effect of the infection, showing a 38.9% recovery in the greenness index. Notably, the severity of infection decreased by 78%, with no detectable silver traces in plant leaves. Biochemical analyses revealed that st-AgNPs treatment resulted in a reduction of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX) activities, as well as lipid peroxidation, compared to non-treated infected plants. These findings suggest that the developed st-AgNPs not only effectively control <em>P. savastanoi</em> infection in soybean but also exhibit the potential to serve as a low-impact, environmentally friendly tool for inclusion in plant disease management protocols across various crops.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103509"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0