Ascites syndrome (AS) in broiler chickens, also known as pulmonary arterial hypertension (PAH), is a significant disease in the poultry industry. It is a nutritional metabolic disease that is closely associated with hypoxia-inducible factors and rapid growth. The rise in pulmonary artery pressure is a crucial characteristic of AS and is instrumental in its development. Hypoxia-inducible factor 1α (HIF-1α) is an active subunit of a key transcription factor in the oxygen-sensing pathway. HIF-1α plays a vital role in oxygen homeostasis and the development of pulmonary hypertension. Studying the effects of HIF-1α on pulmonary hypertension in humans or mammals, as well as ascites in broilers, can help us understand the pathogenesis of AS. Therefore, this review aims to (1) summarize the mechanism of HIF-1α in the development of pulmonary hypertension, (2) provide theoretical significance in explaining the mechanism of HIF-1α in the development of pulmonary arterial hypertension (ascites syndrome) in broilers, and (3) establish the correlation between HIF-1α and pulmonary arterial hypertension (ascites syndrome) in broilers. HIGHLIGHTSExplains the hypoxic mechanism of HIF-1α.Linking HIF-1α to pulmonary hypertension in broilers.Explains the role of microRNAs in pulmonary arterial hypertension in broilers.
{"title":"The relativity analysis of hypoxia inducible factor-1α in pulmonary arterial hypertension (ascites syndrome) in broilers: a review.","authors":"Weile Fang, Enqi Wang, Pei Liu, Xiaona Gao, Xiaolu Hou, Guoliang Hu, Guyue Li, Juan Cheng, Chenxi Jiang, Linjie Yan, Cong Wu, Zheng Xu, Ping Liu","doi":"10.1080/03079457.2024.2358882","DOIUrl":"https://doi.org/10.1080/03079457.2024.2358882","url":null,"abstract":"<p><p>Ascites syndrome (AS) in broiler chickens, also known as pulmonary arterial hypertension (PAH), is a significant disease in the poultry industry. It is a nutritional metabolic disease that is closely associated with hypoxia-inducible factors and rapid growth. The rise in pulmonary artery pressure is a crucial characteristic of AS and is instrumental in its development. Hypoxia-inducible factor 1α (HIF-1α) is an active subunit of a key transcription factor in the oxygen-sensing pathway. HIF-1α plays a vital role in oxygen homeostasis and the development of pulmonary hypertension. Studying the effects of HIF-1α on pulmonary hypertension in humans or mammals, as well as ascites in broilers, can help us understand the pathogenesis of AS. Therefore, this review aims to (1) summarize the mechanism of HIF-1α in the development of pulmonary hypertension, (2) provide theoretical significance in explaining the mechanism of HIF-1α in the development of pulmonary arterial hypertension (ascites syndrome) in broilers, and (3) establish the correlation between HIF-1α and pulmonary arterial hypertension (ascites syndrome) in broilers. HIGHLIGHTSExplains the hypoxic mechanism of HIF-1α.Linking HIF-1α to pulmonary hypertension in broilers.Explains the role of microRNAs in pulmonary arterial hypertension in broilers.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-13DOI: 10.1080/03079457.2024.2358904
Christiaan Ter Veen, Inge M G A Santman-Berends, Marieke Augustijn-Schretlen, Anneke Feberwee
Beside biosecurity, vaccination is important for Mycoplasma synoviae (MS) control as it has been shown to contribute to the reduction of economic impact and, experimentally, also lessens horizontal transmission. In this study, the effect of MS live vaccination on horizontal transmission was quantified under field conditions by analysing 4-year MS monitoring data from non-MS-vaccinated broiler and layer breeders and MS-vaccinated broiler breeders with good biosecurity in single-age housing systems. Flocks were monitored at 20 and 30 weeks of age and every 12 weeks thereafter. At every sampling, 60 blood samples or 24 tracheal swabs were tested using rapid plate agglutination test and ELISA serially or MS DIVA PCR, respectively. The MS incidence rate was calculated and the association with vaccination was analysed by logistic regression. The average MS incidence rate per 1000 weeks was 11.6 cases for non-MS-vaccinated broiler breeders and decreased from 29.6 to 5.6 cases with successive vaccinated production cycles. In non-MS-vaccinated layer breeders it was 3.6. A significant negative association with MS incidence was found after vaccinating four to six successive production cycles compared to non-MS-vaccinated or only one production cycle vaccinated breeders (odds ratio (OR) = 0.23, P = 0.05 & OR = 0.12, P = 0.01, respectively). A significant negative association with MS in non-MS-vaccinated layer breeders (OR = 0.29, P = 0.00) was observed compared to non-MS-vaccinated broiler breeders, possibly due to more controlled contact structures within the layer breeder industry. The results suggest that vaccination and control of contacts contribute to the reduction of between-farm MS transmission.
除了生物安全之外,疫苗接种对于滑膜支原体(MS)控制也很重要,因为疫苗接种有助于减少经济影响,并在实验中减少水平传播。在本研究中,通过分析未接种 MS 疫苗的肉鸡和蛋鸡种鸡以及接种 MS 疫苗且生物安全状况良好的肉鸡种鸡在单龄饲养系统中四年的 MS 监测数据,量化了 MS 活疫苗接种在田间条件下对水平传播的影响。鸡群在 20 周龄和 30 周龄时接受监测,此后每 12 周监测一次。每次采样 60 份血样或 24 份气管拭子,分别使用快速平板凝集试验和 ELISA 血清学方法或 MS DIVA PCR 方法进行检测。计算多发性硬化症发病率,并通过逻辑回归分析与疫苗接种的关系。未接种 MS 疫苗的肉种鸡的 MS 平均发病率为每 1000 周 11.6 例,随着接种疫苗生产周期的连续进行,发病率从 29.6 例降至 5.6 例。在未接种疫苗的蛋鸡种鸡中,发病率为 3.6。与未接种疫苗或仅接种一个生产周期疫苗的种鸡相比,连续接种四至六个生产周期疫苗的种鸡多发性硬化症发病率呈明显的负相关(几率比(OR)= 0.23,P = 0.05 和 OR = 0.12,P = 0.01)。与未接种疫苗的肉鸡饲养者相比,未接种疫苗的蛋鸡饲养者与多发性硬化症呈明显的负相关(OR = 0.29,P = 0.00),这可能是由于蛋鸡饲养业中的接触结构受到了更严格的控制。结果表明,接种疫苗和控制接触有助于减少多发性硬化症在养殖场之间的传播。
{"title":"Quantification of the effect of vaccination on the control of horizontal transmission of <i>Mycoplasma synoviae</i> under field conditions.","authors":"Christiaan Ter Veen, Inge M G A Santman-Berends, Marieke Augustijn-Schretlen, Anneke Feberwee","doi":"10.1080/03079457.2024.2358904","DOIUrl":"10.1080/03079457.2024.2358904","url":null,"abstract":"<p><p>Beside biosecurity, vaccination is important for <i>Mycoplasma synoviae</i> (MS) control as it has been shown to contribute to the reduction of economic impact and, experimentally, also lessens horizontal transmission. In this study, the effect of MS live vaccination on horizontal transmission was quantified under field conditions by analysing 4-year MS monitoring data from non-MS-vaccinated broiler and layer breeders and MS-vaccinated broiler breeders with good biosecurity in single-age housing systems. Flocks were monitored at 20 and 30 weeks of age and every 12 weeks thereafter. At every sampling, 60 blood samples or 24 tracheal swabs were tested using rapid plate agglutination test and ELISA serially or MS DIVA PCR, respectively. The MS incidence rate was calculated and the association with vaccination was analysed by logistic regression. The average MS incidence rate per 1000 weeks was 11.6 cases for non-MS-vaccinated broiler breeders and decreased from 29.6 to 5.6 cases with successive vaccinated production cycles. In non-MS-vaccinated layer breeders it was 3.6. A significant negative association with MS incidence was found after vaccinating four to six successive production cycles compared to non-MS-vaccinated or only one production cycle vaccinated breeders (odds ratio (OR) = 0.23, <i>P</i> = 0.05 & OR = 0.12, <i>P</i> = 0.01, respectively). A significant negative association with MS in non-MS-vaccinated layer breeders (OR = 0.29, <i>P</i> = 0.00) was observed compared to non-MS-vaccinated broiler breeders, possibly due to more controlled contact structures within the layer breeder industry. The results suggest that vaccination and control of contacts contribute to the reduction of between-farm MS transmission.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2024-02-14DOI: 10.1080/03079457.2024.2312116
Tae-Hyeon Kim, Andrew Y Cho, Sun-Hak Lee, Jei-Hyun Jeong, Chang-Seon Song, Justin Bahl, Dong-Hun Lee
We report the first North American origin class I avian orthoavulavirus 1 (AOAV-1) isolated from a faecal dropping of wild Eurasian teal (Anas crecca) in South Korea. Whole genome sequencing and comparative phylogenetic analysis revealed that the AOAV-1/Eurasian teal/South Korea/KU1405-3/2017 virus belongs to the sub-genotype 1.2 of class I AOAV-1. Phylogenetic analysis suggested multiple introductions of the North American sub-genotype 1.2 viruses into Asia and its establishment in the wild bird population in East Asia since May 2011. These results provide information on the epidemiology of AOAV-1, particularly the role of migratory wild birds in exchanging viruses between the Eurasian and North American continents. Enhanced genomic surveillance is required to improve our understanding on the evolution and transmission dynamics of AOAV-1 in wild birds.
{"title":"Isolation and whole genome sequencing of North American lineage class I avian orthoavulavirus 1 isolated from wild Eurasian teal in South Korea.","authors":"Tae-Hyeon Kim, Andrew Y Cho, Sun-Hak Lee, Jei-Hyun Jeong, Chang-Seon Song, Justin Bahl, Dong-Hun Lee","doi":"10.1080/03079457.2024.2312116","DOIUrl":"10.1080/03079457.2024.2312116","url":null,"abstract":"<p><p>We report the first North American origin class I avian orthoavulavirus 1 (AOAV-1) isolated from a faecal dropping of wild Eurasian teal (<i>Anas crecca</i>) in South Korea. Whole genome sequencing and comparative phylogenetic analysis revealed that the AOAV-1/Eurasian teal/South Korea/KU1405-3/2017 virus belongs to the sub-genotype 1.2 of class I AOAV-1. Phylogenetic analysis suggested multiple introductions of the North American sub-genotype 1.2 viruses into Asia and its establishment in the wild bird population in East Asia since May 2011. These results provide information on the epidemiology of AOAV-1, particularly the role of migratory wild birds in exchanging viruses between the Eurasian and North American continents. Enhanced genomic surveillance is required to improve our understanding on the evolution and transmission dynamics of AOAV-1 in wild birds.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139575103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2024-01-22DOI: 10.1080/03079457.2024.2304005
Käthe Elise Kittelsen, Guro Vasdal, Ida Thøfner, Fernanda Tahamtani
The two most common animal-based indicators used to evaluate leg health in broiler chickens are footpad dermatitis (FPD) and gait scoring, but these indicators are less explored in broiler breeders. This study is the first to investigate FPD and gait scoring in broiler breeders during their lifespan from rearing to end of life. In total, eight flocks were monitored (four Ross and four Hubbard) at five different timepoints, in rearing (5 and 15 weeks of age), during the production period (25 and 45 weeks of age) and at the end of the production period (approximately 60 weeks of age). At each visit, 50 hens and 25 roosters were gait scored (six-point scale) and footpads from another 50 hens and 25 roosters were evaluated (five-point scale) (total n = 3000 breeders, 2000 hens and 1000 roosters). Litter quality and air quality were measured at each visit. The results showed that the overall prevalence of FPD in rearing was low and that it increased towards the end of the production, with a mean FPD score of 2 out of a maximum 4 in the hens, indicating moderate lesions and 1.5 in the roosters. In all houses, the litter was dry and loose. FPD was not related to the litter quality, but to air quality, especially the ammonia concentration (P < 0.001). Overall, the gait score were good, and increased with age in both hens (P < 0.001) and roosters of both hybrids (P < 0.001).
{"title":"A walk through the broiler breeder life: how do footpad dermatitis and gait scores develop from rearing to slaughter?","authors":"Käthe Elise Kittelsen, Guro Vasdal, Ida Thøfner, Fernanda Tahamtani","doi":"10.1080/03079457.2024.2304005","DOIUrl":"10.1080/03079457.2024.2304005","url":null,"abstract":"<p><p>The two most common animal-based indicators used to evaluate leg health in broiler chickens are footpad dermatitis (FPD) and gait scoring, but these indicators are less explored in broiler breeders. This study is the first to investigate FPD and gait scoring in broiler breeders during their lifespan from rearing to end of life. In total, eight flocks were monitored (four Ross and four Hubbard) at five different timepoints, in rearing (5 and 15 weeks of age), during the production period (25 and 45 weeks of age) and at the end of the production period (approximately 60 weeks of age). At each visit, 50 hens and 25 roosters were gait scored (six-point scale) and footpads from another 50 hens and 25 roosters were evaluated (five-point scale) (total <i>n</i> = 3000 breeders, 2000 hens and 1000 roosters). Litter quality and air quality were measured at each visit. The results showed that the overall prevalence of FPD in rearing was low and that it increased towards the end of the production, with a mean FPD score of 2 out of a maximum 4 in the hens, indicating moderate lesions and 1.5 in the roosters. In all houses, the litter was dry and loose. FPD was not related to the litter quality, but to air quality, especially the ammonia concentration (<i>P</i> < 0.001). Overall, the gait score were good, and increased with age in both hens (<i>P</i> < 0.001) and roosters of both hybrids (<i>P</i> < 0.001).</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139401630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2024-01-11DOI: 10.1080/03079457.2024.2302138
Bingrong Wu, Xiaoning Jiang, Dalin He, Feng Wei, Mingtian Mao, YuDong Zhu, Hong Su, Yi Tang, Youxiang Diao
Research highlights: Samples of suspected FAdV-infected waterfowl from farms in Shandong Province were collected from 2019 to 2022.Single infections with FAdV were less frequent than mixed infections.477 out of 792 samples (60.23%) tested positive for FAdV nucleic acids.Detection rate of FAdV was 65.47% in fattening duck farms, 55.73% in breeder duck farms and 54.55% in fattening geese farms.
{"title":"Epidemiological investigation of fowl adenovirus (FAdV) infections in ducks and geese in Shandong Province, China.","authors":"Bingrong Wu, Xiaoning Jiang, Dalin He, Feng Wei, Mingtian Mao, YuDong Zhu, Hong Su, Yi Tang, Youxiang Diao","doi":"10.1080/03079457.2024.2302138","DOIUrl":"10.1080/03079457.2024.2302138","url":null,"abstract":"<p><strong>Research highlights: </strong>Samples of suspected FAdV-infected waterfowl from farms in Shandong Province were collected from 2019 to 2022.Single infections with FAdV were less frequent than mixed infections.477 out of 792 samples (60.23%) tested positive for FAdV nucleic acids.Detection rate of FAdV was 65.47% in fattening duck farms, 55.73% in breeder duck farms and 54.55% in fattening geese farms.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this trial was to evaluate how broilers responded to Aspergillus flavus strains that are toxigenic and atoxigenic. The study included four treatments in a 2 × 2 factorial design, with six replicates of 10 birds each. As a result of this study measuring feed intake (FI), weight gain (WG), feed conversion ratio (FCR), crude protein, ether extract, and crude fibre, the interaction was insignificant between the toxigenic and atoxigenic diets (P > 0.05). Consumption of toxigenic aflatoxin B1-500 ppb diet decreased FI and WG but increased FCR, and cost to produce live broiler weight (P < 0.05) compared to the control diets. The addition of atoxigenic strains to contaminated diets significantly offset (P < 0.05) the effects. Diets with or without 500 ppb toxigenic and atoxigenic A. flavus did not affect the relative weight g/100gBW of pancreas, gizzard and bursa of Fabricius. Dietary inclusion of 500 ppb toxigenic Aspergillus spp. increased the relative weight (P < 0.05) of the kidney, liver, spleen and thymus while atoxigenic dietary addition reduced the relative weight of the same organs (P < 0.05). Dietary inclusion of toxigenic and atoxigenic Aspergillus spp. did not significantly affect the haematological parameters measured (P < 0.05). Dietary inclusion of 500 ppb toxigenic Aspergillus elevated the urea, creatine, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in the serum of the broilers (P < 0.05). A decrease was observed when atox igenic A. flavus was used in the intervention for urea, creatinine and AST (P < 0.05), whereas an insignificant reduction was observed for ALT and ALP (P ≤ 0.05). This study concluded that dietary atoxigenic strain improved broiler performance, digestibility, and blood parameters.
{"title":"Response of broilers to dietary inclusion of atoxigenic <i>Aspergillus flavus</i> strain as a biocontrol strategy of aflatoxin.","authors":"Abiola Olayemi Salako, Job Olutimehin Atteh, Taiwo Oladoye Akande, Isiaka Oyeniyi Kolade, Eunice Tayo Bajomo, Adejoke Adegoke","doi":"10.1080/03079457.2024.2316025","DOIUrl":"10.1080/03079457.2024.2316025","url":null,"abstract":"<p><p>The objective of this trial was to evaluate how broilers responded to <i>Aspergillus flavus</i> strains that are toxigenic and atoxigenic. The study included four treatments in a 2 × 2 factorial design, with six replicates of 10 birds each. As a result of this study measuring feed intake (FI), weight gain (WG), feed conversion ratio (FCR), crude protein, ether extract, and crude fibre, the interaction was insignificant between the toxigenic and atoxigenic diets (<i>P</i> > 0.05). Consumption of toxigenic aflatoxin B1-500 ppb diet decreased FI and WG but increased FCR, and cost to produce live broiler weight (<i>P</i> < 0.05) compared to the control diets. The addition of atoxigenic strains to contaminated diets significantly offset (<i>P</i> < 0.05) the effects. Diets with or without 500 ppb toxigenic and atoxigenic <i>A. flavus</i> did not affect the relative weight g/100gBW of pancreas, gizzard and bursa of Fabricius. Dietary inclusion of 500 ppb toxigenic <i>Aspergillus</i> spp. increased the relative weight (<i>P</i> < 0.05) of the kidney, liver, spleen and thymus while atoxigenic dietary addition reduced the relative weight of the same organs (<i>P</i> < 0.05). Dietary inclusion of toxigenic and atoxigenic <i>Aspergillus</i> spp. did not significantly affect the haematological parameters measured (<i>P</i> < 0.05). Dietary inclusion of 500 ppb toxigenic <i>Aspergillus</i> elevated the urea, creatine, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in the serum of the broilers (<i>P</i> < 0.05). A decrease was observed when atox igenic <i>A. flavus</i> was used in the intervention for urea, creatinine and AST (<i>P</i> < 0.05), whereas an insignificant reduction was observed for ALT and ALP (<i>P</i> ≤ 0.05). This study concluded that dietary atoxigenic strain improved broiler performance, digestibility, and blood parameters.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139691131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2024-02-09DOI: 10.1080/03079457.2024.2307567
Victória Veiga Alves, Letícia Cury Rocha Veloso Arantes, Dayse Helena Lages da Silva, Eric Santos Oliveira, Julia Figueiredo de Souza, Mailson Teixeira da Silva, Matheus Dias Araújo, Rodrigo Dias de Oliveira Carvalho, João Luís Reis Cunha, Leonardo José Camargos Lara, Roselene Ecco, Nelson Rodrigo da Silva Martins, Paul Andrew Barrow, Oliveiro Caetano de Freitas Neto
Research highlights: Peptides + CpG-ODN reduced SH in caeca at the first week post-infection.Administered formulations did not reduce SH-faecal excretion.Levels of intestinal IgA were similar between all groups.CpG-ODN improved some parameters associated with chick intestinal health.
摘要 海德堡沙门氏菌(Salmonella Heidelberg,SH)致病性和抗菌性分离株的日益流行是一个全球关注的问题。因此,需要采取新的策略来预防和控制由这种细菌引起的感染。本研究旨在探讨单独或与含有胞嘧啶-磷酸二酯-鸟嘌呤(CpG-ODN)的寡脱氧核苷酸联合使用以细菌肽为基础的配方进行体内注射对雏鸡感染海德堡沙门氏菌的保护作用。通过分析公共数据库中的 SH 基因组数据,筛选出结构蛋白的氨基酸序列或与肠道定植更相关的氨基酸序列。对这些序列进行线性表位预测,以确定高免疫原性肽。对 180 枚 SPF 卵进行孵化,并在孵化第 18 天通过尿囊腔注射。结果表明,在感染后的第一周,卵内注射肽+CpG-ODN可减少SH在盲肠内容物中的定植,但在整个研究过程中并没有减少粪便的总体排泄量。此外,CpG-ODN注射可能会对肠道健康产生积极影响,在21 dpi时隐窝深度降低,28 dpi时绒毛高度增加就是证明。各组雏鸡的分泌型 IgA 水平没有差异,在整个研究过程中,任何感染组的雏鸡肝脏中都没有发现可检测到的 SH 计数。总之,肽+CpG-ODN的体内注射可能有助于在感染早期减少SH在盲肠中的定植,但不会影响粪便的总排泄量。此外,CpG-ODN注射液可能会改善雏鸡的肠道健康参数。
{"title":"Effects of <i>in ovo</i> injection of bacterial peptides and CpG-ODN on <i>Salmonella enterica</i> serovar Heidelberg infection in specific pathogen-free (SPF) chicks.","authors":"Victória Veiga Alves, Letícia Cury Rocha Veloso Arantes, Dayse Helena Lages da Silva, Eric Santos Oliveira, Julia Figueiredo de Souza, Mailson Teixeira da Silva, Matheus Dias Araújo, Rodrigo Dias de Oliveira Carvalho, João Luís Reis Cunha, Leonardo José Camargos Lara, Roselene Ecco, Nelson Rodrigo da Silva Martins, Paul Andrew Barrow, Oliveiro Caetano de Freitas Neto","doi":"10.1080/03079457.2024.2307567","DOIUrl":"10.1080/03079457.2024.2307567","url":null,"abstract":"<p><strong>Research highlights: </strong>Peptides + CpG-ODN reduced SH in caeca at the first week post-infection.Administered formulations did not reduce SH-faecal excretion.Levels of intestinal IgA were similar between all groups.CpG-ODN improved some parameters associated with chick intestinal health.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139490029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2024-01-25DOI: 10.1080/03079457.2024.2304628
Amir Hossein Pourabadeh, Seyed Ahmad Madani, Ruhollah Dorostkar, Mohammad Rezaeian, Hossein Esmaeili, Masoumeh Bolandian, Ali Salavati, Seyed Mohammad Mahdi Hashemian, Arezoo Aghahasani
Research highlights: Bacteriophage (BP) cocktail was partially resistant to different temperatures and pH values.The BP cocktail showed lytic effects on different Salmonella isolates.The BP cocktail reduced Salmonella colonization in the internal organs of broilers.
{"title":"Evaluation of the <i>in vitro</i> and <i>in vivo</i> efficiency of in-feed bacteriophage cocktail application to control <i>Salmonella</i> Typhimurium and <i>Salmonella</i> Enteritidis infection in broiler chicks.","authors":"Amir Hossein Pourabadeh, Seyed Ahmad Madani, Ruhollah Dorostkar, Mohammad Rezaeian, Hossein Esmaeili, Masoumeh Bolandian, Ali Salavati, Seyed Mohammad Mahdi Hashemian, Arezoo Aghahasani","doi":"10.1080/03079457.2024.2304628","DOIUrl":"10.1080/03079457.2024.2304628","url":null,"abstract":"<p><strong>Research highlights: </strong>Bacteriophage (BP) cocktail was partially resistant to different temperatures and pH values.The BP cocktail showed lytic effects on different <i>Salmonella</i> isolates.The BP cocktail reduced <i>Salmonella</i> colonization in the internal organs of broilers.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01Epub Date: 2024-03-19DOI: 10.1080/03079457.2024.2312133
Marwa I Abdel Haleem, Hanem F Khater, Shimaa N Edris, Hanan A A Taie, Samah M Abdel Gawad, Nibal A Hassan, Ali H El-Far, Yasmeen Magdy, Sawsan S Elbasuni
Although anticoccidial drugs have been used to treat avian coccidiosis for nearly a century, resistance, bird harm, and food residues have caused health concerns. Thus, Nannochloropsis oculata was investigated as a possible coccidiosis treatment for broilers. A total of 150 1-day-old male Cobb broiler chicks were treated as follows: G1-Ng: fed a basal diet; G2-Ps: challenged with Eimeria spp. oocysts and fed basal diet; G3-Clo: challenged and fed basal diet with clopidol; G4-NOa: challenged and fed 0.1% N. oculata in diet, and G5-NOb: challenged and fed 0.2% N. oculata. Compared to G2-Ps, N. oculata in the diet significantly (P < 0.05) decreased dropping scores, lesion scores, and oocyst shedding. Without affecting breast meat colour metrics, N. oculata improved meat quality characters. At 28 days of age, birds received 0.2% N. oculata had significantly (P < 0.05) higher serum levels of MDA, T-SOD, HDL, and LDL cholesterol compared to G2-Ps. Serum AST, ALT, and urea levels were all decreased when N. oculata (0.2%) was used as opposed to G2-Ps. Histopathological alterations and the number of developmental and degenerative stages of Eimeria spp. in the intestinal epithelium were dramatically reduced by 0.2% N. oculata compared to G2-Ps. Molecular docking revealed a higher binding affinity of N. oculata for E. tenella aldolase, EtAMA1, and EtMIC3, which hindered glucose metabolism, host cell adhesion, and invasion of Eimeria. Finally, N. oculata (0.2%) can be used in broiler diets to mitigate the deleterious effects of coccidiosis.
{"title":"Bioefficacy of dietary inclusion of <i>Nannochloropsis oculata</i> on <i>Eimeria</i> spp. challenged chicks: clinical approaches, meat quality, and molecular docking.","authors":"Marwa I Abdel Haleem, Hanem F Khater, Shimaa N Edris, Hanan A A Taie, Samah M Abdel Gawad, Nibal A Hassan, Ali H El-Far, Yasmeen Magdy, Sawsan S Elbasuni","doi":"10.1080/03079457.2024.2312133","DOIUrl":"10.1080/03079457.2024.2312133","url":null,"abstract":"<p><p>Although anticoccidial drugs have been used to treat avian coccidiosis for nearly a century, resistance, bird harm, and food residues have caused health concerns. Thus, <i>Nannochloropsis oculata</i> was investigated as a possible coccidiosis treatment for broilers. A total of 150 1-day-old male Cobb broiler chicks were treated as follows: G1-Ng: fed a basal diet; G2-Ps: challenged with <i>Eimeria</i> spp. oocysts and fed basal diet; G3-Clo: challenged and fed basal diet with clopidol; G4-NOa: challenged and fed 0.1% <i>N. oculata</i> in diet, and G5-NOb: challenged and fed 0.2% <i>N. oculata</i>. Compared to G2-Ps, <i>N. oculata</i> in the diet significantly (<i>P</i> < 0.05) decreased dropping scores, lesion scores, and oocyst shedding. Without affecting breast meat colour metrics, <i>N. oculata</i> improved meat quality characters. At 28 days of age, birds received 0.2% <i>N. oculata</i> had significantly (<i>P</i> < 0.05) higher serum levels of MDA, T-SOD, HDL, and LDL cholesterol compared to G2-Ps. Serum AST, ALT, and urea levels were all decreased when <i>N. oculata</i> (0.2%) was used as opposed to G2-Ps. Histopathological alterations and the number of developmental and degenerative stages of <i>Eimeria</i> spp. in the intestinal epithelium were dramatically reduced by 0.2% <i>N. oculata</i> compared to G2-Ps. Molecular docking revealed a higher binding affinity of <i>N. oculata</i> for <i>E. tenella</i> aldolase, EtAMA1, and EtMIC3, which hindered glucose metabolism, host cell adhesion, and invasion of <i>Eimeria</i>. Finally, <i>N. oculata</i> (0.2%) can be used in broiler diets to mitigate the deleterious effects of coccidiosis.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139575099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}