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Clinical, Hematological and Biochemical Studies on Feline Enteritis 猫肠炎的临床、血液学和生化研究
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.279370.1799
A. Sobeih, Hossam Eldin El Attar, Yassen Abdel-Raoof, mohammed anwar ramadan, M. Helal
Keywords Feline enteritis has significant clinical issues that have an impact on their health. We conducted this study on 30 cats suffering from various types of enteritis affection, including viral, bacterial, and parasitic. The study aimed to assess the clinical, hematological and biochemical alterations in cats with various forms of enteritis. The most prevalent clinical signs seen in all forms of enteritis were diarrhea, vomiting, dehydration, and anorexia. Hematological changes revealed a significant reduction in hemoglobin content and red blood cell count than control values in viral and bacterial enteritis. WBCs count and granulocytes were significantly increased in bacterial enteritis. In viral enteritis, there was a significant decrease in lymphocytes and granulocytes, while in parasitic enteritis, there was a significant increase in eosinophils. Different types of enteritis showed significant increases in total protein, globulin, ALT, ALP, and AST, as well as BUN and creatinine, and significant decreases in Ca, P, Na, Cl, and K ions. There was a significant increase in antioxidant enzyme levels, including GSH-Px and SOD, and a significant increase in MDA, inflammatory cytokines, including IL2 and IL6, and acute phase proteins, including HP and CRP, in all affections. These results highlight the complexity of feline enteritis and the demand for specialized treatment strategies to meet the wide range of clinical, hematological and biochemical changes..
关键词 猫肠炎是影响猫健康的重大临床问题。我们对 30 只患有各种类型肠炎(包括病毒性、细菌性和寄生虫性)的猫进行了研究。研究旨在评估患有各种类型肠炎的猫的临床、血液学和生化变化。各种肠炎最常见的临床症状是腹泻、呕吐、脱水和厌食。血液学变化显示,在病毒性和细菌性肠炎中,血红蛋白含量和红细胞计数比对照值明显降低。细菌性肠炎患者的白细胞计数和粒细胞明显增加。在病毒性肠炎中,淋巴细胞和粒细胞明显减少,而在寄生虫性肠炎中,嗜酸性粒细胞明显增加。不同类型的肠炎显示总蛋白、球蛋白、ALT、ALP 和 AST 以及 BUN 和肌酐显著增加,而 Ca、P、Na、Cl 和 K 离子显著减少。在所有病症中,抗氧化酶(包括 GSH-Px 和 SOD)水平都有明显升高,而 MDA、炎症细胞因子(包括 IL2 和 IL6)以及急性期蛋白(包括 HP 和 CRP)都有明显升高。这些结果突显了猫肠炎的复杂性,以及对专门治疗策略的需求,以满足广泛的临床、血液学和生化变化。
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引用次数: 0
Mesenchymal stem cells promote the repair of chemotherapy-induced premature ovarian failure in female rats 间充质干细胞促进化疗引起的雌性大鼠卵巢早衰的修复
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.283972.1814
Heba Elsaied Sherif, Y. E. El- Senosi, S. Hussein, Ehab Elzoghby, Mohamed Ahmed Sobh, Sara M. Farrag, Basma Hamed Osman
Keywords Chemotherapeutic drugs, particularly alkylating cytotoxics such as cyclophosphamide (CYP) play a great job in inducing premature ovarian failure (POF). Hormone replacement therapy (HRT) is a widely used medication to improve hormone secretion. However, long-term HRT increases the risk of breast cancer and cardiovascular disease is concerning. Therefore, there is an urgent need to develop a safe and effective treatment for POF. Bone Marrow-(BM-MSCs) and Umbilical Cord -(UC-MSCs) derived stem cells (SCs) were isolated and identified from healthy rats. For POF induction, CYP (200mg/Kg b.wt) injected (i.p) into CYP, CYP+BMSCs, and CYP+UCMSCs followed by another dose (10mg/ b.wt, i.p) after one week. The normal control (NC) group were injected with saline. After two weeks of POF induction MSCs (1 × 10 6 cells/ml) of media were transplanted into POF rats through tail-vein. The effects of MSCs transplantation to POF were evaluated through sex hormones, iron, and complete blood count (CBC) analysis. POF-induced rats showed a significant increase in serum FSH and LH, with marked decrease in anti-Mullerian hormone (AMH) and estradiol (E2). Obvious increase in serum iron level observed in CYP group compared to the NC. Moreover, POF-induced rats displayed leucopenia, erythrocytopenia, and thrombocytopenia with a significant reduction in Hb, PCV, MCV, MCH, and MCHC compared to the NC. Administration of MSCs to CYP-injured rats significantly improved female reproductive hormones disturbances, iron, and hemogram. It was suggested that MSCs was a potential approach for improving sex hormones, iron, and hematological alterations, and potentially promoting the restoration of CYP-induced POF. Cyclophosphamide
关键词 化疗药物,尤其是环磷酰胺(CYP)等烷基化细胞毒性药物在诱发卵巢早衰(POF)方面发挥着重要作用。激素替代疗法(HRT)是一种广泛使用的改善激素分泌的药物。然而,长期使用激素替代疗法会增加罹患乳腺癌和心血管疾病的风险,这一点令人担忧。因此,开发一种安全有效的 POF 治疗方法迫在眉睫。我们从健康大鼠体内分离并鉴定了骨髓干细胞(BM-MSCs)和脐带干细胞(UC-MSCs)。为了诱导 POF,CYP、CYP+BM-MSCs 和 CYP+UCMSCs 组分别注射 200 毫克/千克体重的 CYP(i.p),一周后再注射另一剂量(10 毫克/体重,i.p)。正常对照(NC)组注射生理盐水。POF 诱导两周后,将培养基中的间充质干细胞(1 × 10 6 cells/ml)通过尾静脉移植到 POF 大鼠体内。通过性激素、铁和全血细胞计数(CBC)分析评估了间充质干细胞移植对 POF 的影响。POF诱导的大鼠血清FSH和LH显著增加,抗苗勒氏管激素(AMH)和雌二醇(E2)明显下降。与 NC 相比,CYP 组的血清铁含量明显增加。此外,与 NC 相比,POF 诱导的大鼠表现出白细胞减少、红细胞减少和血小板减少,Hb、PCV、MCV、MCH 和 MCHC 显著降低。给 CYP 损伤大鼠注射间充质干细胞可明显改善雌性生殖激素紊乱、铁和血型图。这表明间充质干细胞是改善性激素、铁和血液学改变的一种潜在方法,并有可能促进 CYP 诱导的 POF 的恢复。环磷酰胺
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引用次数: 0
Quality and chemical composition of meat recovered from bovine carcasses lightly infested with cysticercosis 从轻度囊尾蚴病牛屠体中提取的肉的质量和化学成分
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.284390.1815
Mohamed A. Abd El-Hameed, Hemmat M. Ibrahim, Reham A. Amin
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引用次数: 0
Soybean 11S globulin spray boosts chilled shrimp shelf-life, physicochemical, and sensory attributes beyond twelve days 喷洒大豆 11S 球蛋白可提高冷冻虾的货架期、理化和感官属性,使其超过 12 天
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.283262.1808
Wesam Dawam, Mai Elsheikh, Shimaa N. Edris, Ali Osman, M. Sitohy, I. Sabike
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引用次数: 0
Prevalence of some Foodborne Pathogens (FBPs) in imported chilled and frozen beef in Egypt 埃及进口冷藏和冷冻牛肉中某些食源性病原体(FBPs)的流行情况
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.280836.1803
Aya Mohamed Ahmed, F. Mohamed, Gehan Sayed eltanany, Shimaa N. Edris
Keywords Food safety is a global priority due to foodborne pathogens contamination. This study determined the prevalence of Aerobic mesophilic bacteria count (AMB), Staphylococcus aureus, Salmonella spp., E. coli and Listeria monocytogenes in imported chilled and frozen beef in Egypt. The experimental results revealed that the AMB (cfu/g) in chilled samples varied from 5.3×10 4 to 4.7×10 6 , with an average of 8.35×10 5 ±1.06. In contrast, for frozen samples, the range was from 9.7×10 3 to 1.5×10 6 , with an average of 3.11×10 5 ±0.29. Staphylococcus aureus was found in 24 % of chilled beef samples and 16 % of imported frozen beef samples. The prevalence of Salmonella spp. was identified in 5% of chilled beef samples and 3% of imported frozen beef samples . Additionally, Escherichia coli was identified in and 14% of chilled samples and 8% of frozen samples. The presence of L. monocytogenes was identified in 3% of chilled beef samples and 1% of frozen samples. The results obtained validated the inadequate bacteriological quality of certain imported chilled and frozen meats sold in the markets of Cairo and Qalyubia. This quality deficiency is a result of unclean transportation practices that continue to the retail levels. Chilled comprehensive findings, it can be inferred that imported chilled and frozen beef poses a substantial bacteriological public health risk and requires specific control measures.
关键词 由于食源性致病菌污染,食品安全是全球优先考虑的问题。本研究测定了埃及进口冷藏和冷冻牛肉中需氧中嗜性细菌计数(AMB)、金黄色葡萄球菌、沙门氏菌属、大肠杆菌和李斯特菌的流行率。实验结果表明,冷藏样品中的 AMB(cfu/g)从 5.3×10 4 到 4.7×10 6 不等,平均值为 8.35×10 5 ±1.06。相比之下,冷冻样本中的菌落总数从 9.7×10 3 到 1.5×10 6 不等,平均为 3.11×10 5 ±0.29。在 24% 的冷藏牛肉样本和 16% 的进口冷冻牛肉样本中发现了金黄色葡萄球菌。在 5%的冰鲜牛肉样本和 3%的进口冷冻牛肉样本中发现了沙门氏菌。此外,在 14% 的冰鲜牛肉样本和 8% 的冷冻牛肉样本中发现了大肠埃希氏菌。在 3% 的冷藏牛肉样本和 1% 的冷冻牛肉样本中发现了单核细胞增生酵母菌。这些结果证实了开罗和卡留比亚市场上销售的某些进口冷藏和冷冻肉类的细菌学质量不合格。造成这种质量缺陷的原因是不清洁的运输方式一直延续到零售环节。根据冰鲜牛肉的综合调查结果,可以推断进口冰鲜牛肉和冷冻牛肉对公众健康构成了严重的细菌风险,需要采取具体的控制措施。
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引用次数: 0
The prevalence detection of Cysticercus bovis in slaughtered cattle in El-Bassatine abattoir, Cairo, Egypt 在埃及开罗 El-Bassatine 屠宰场屠宰的牛中检测囊尾蚴的流行情况
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.283865.1813
Mohamed A. Abd El-Hameed, Hemmat M. Ibrahim, Reham A. Amin
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引用次数: 0
Cowpea 11S globulin spray quadruples shrimp shelf-life and maintains freshness 豇豆 11S 球蛋白喷雾剂可将虾的货架期延长四倍并保持新鲜
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.282792.1807
Mai Elsheikh, Wesam Dawam, Shimaa N. Edris, Ali Osman, M. Sitohy, I. Sabike
Keywords Shrimp preservation is of interest to food scientists and processors because of their restricted shelf life caused by microbial spoilage, black spot formation, and the associated financial losses and food safety risks. Current study was conducted to determine the preservative impacts of Cowpea ( Vigna unguiculata ) 11S globulin (CPEG) spray on tiger shrimp ( Penaeus monodon ) shelf-life and physicochemical characteristics, as well as optimum field preservative levels. Fresh peeled tiger shrimp were sprayed with either 100µg/ml (CPEG1) or 200µg/ml (CPEG2) of CPEG at a rate of 2 ml/100 gm and refrigerated at 2±0.5°C. The control shrimp received only sterile distilled water. After 12 storage days, CPEG2 sprayed shrimp displayed a steadily raising pH trend, reaching 7.3, whereas control and CPEG1 sprayed shrimp demonstrated a V-pH curve. Though was not entirely significant, but 200 µg/ml of CPEG spray improved shrimp WHC and drip loss attributes. Compared to the apparent increasing curves of control shrimp's native microbial curves, which culminated in clear spoiling on the eighth day, CPEG-treated shrimp at both doses did not exceed 6 log cfu/gm for the entire twelve-day chilling period. Also, both CPEG levels exhibited a significant antioxidant impact compared to the control. The retarding effect of CPEG on treated shrimp native microbial curves and malondialdehyde was dose dependent. Conclusively, both CPEG levels kept the fresh tiger shrimp's organoleptic aspects and postponed the spoilage beyond twelve-days. Therefore, the shrimp's shelf-life can potentially be extended while preserving its safety and quality by using the CPEG spray, which works best at 200 µg/ml.
关键词 虾类保鲜是食品科学家和加工商感兴趣的问题,因为微生物腐败、黑斑形成以及相关的经济损失和食品安全风险会限制虾类的货架期。本研究旨在确定豇豆(Vigna unguiculata)11S球蛋白(CPEG)喷雾剂对虎虾(Penaeus monodon)货架期和理化特性的防腐影响,以及最佳田间防腐剂水平。新鲜去皮的虎虾按 2 ml/100 gm 的比例喷洒 100µg/ml (CPEG1)或 200µg/ml (CPEG2)的 CPEG,并在 2±0.5°C 下冷藏。对照组虾只接受无菌蒸馏水。储存 12 天后,喷洒 CPEG2 的虾的 pH 值呈稳步上升趋势,达到 7.3,而对照组和喷洒 CPEG1 的虾的 pH 值呈 V 型曲线。虽然不完全显著,但 200 µg/ml 的 CPEG 喷雾剂改善了虾的 WHC 和滴水损失属性。对照组对虾的原生微生物曲线明显呈上升趋势,在第八天达到腐败的顶峰,相比之下,两种剂量的 CPEG 处理过的对虾在整个 12 天的冷冻期都没有超过 6 log cfu/gm。此外,与对照组相比,两种浓度的 CPEG 都具有显著的抗氧化作用。CPEG 对处理过的虾的本地微生物曲线和丙二醛的延缓作用与剂量有关。总之,两种浓度的 CPEG 都能保持鲜虎虾的感官特性,并能延缓 12 天后的腐烂变质。因此,使用 CPEG 喷雾剂可延长虾的保质期,同时保持其安全和质量。
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引用次数: 0
Sanacore® GM supplemented feed boosts the haemato-biochemical indices, immune response, anti-oxidative profile, and defense against Aeromonas Vernoii in Nile tilapia (Oreochromis niloticus). 添加 Sanacore® GM 的饲料可提高尼罗罗非鱼(Oreochromis niloticus)的血液生化指标、免疫反应、抗氧化能力以及对 Aeromonas Vernoii 的防御能力。
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.270979.1790
hadeer youseef, Eman Araby, Amel El Asely, A. Shaheen, Amany Abbass
Keywords The present research was carried out to evaluate the effect of Sanacore ® GM feed additives on the growth rate, hematological indices, biochemical indicators, anti-oxidative profile, non-specific immune response, and defense of Nile tilapia against Aeromonas veronii ( A. veronii ). Two fish groups of mean initial weight (13.5 ± 1.5g) were randomly allocated in triplicate and fed Sanacore ® GM at a dose of 0 (control) and 5 g/kg diet for a period of 30 days. Fish were challenged by A. veronii at the experimental endpoint and the mortality rate was recorded for 15 days. Fish fed Sanacore ® GM supplemented diet revealed significant increase in final body weight, haemato-biochemical indices (red blood cells, white blood cells, hemoglobin concentration, lymphocytes, neutrophils, serum total protein, and albumin levels), and immunological parameters (phagocytic activity, nitric oxide, lysozyme activity, IgM, and serum bactericidal activity). In addition, the anti-oxidative enzymes activity (superoxide dismutase and glutathione peroxidase) showed meaningful increase in Nile tilapia received Sanacore ® GM incorporated diet. However, catalase enzyme displayed no significant activity ( P > 0.05) in the supplemented group compared to the control group. The relative percentage survival was 100% in the challenged Sanacore ® GM group compared the control. Our finding suggested that Sanacore ® GM might considerably boost final growth rate, haemato-biochemical indices, immune response, anti-oxidative capability of Nile tilapia, as well as disease resistant against pathogenic strains of A. veronii . Aeromonas veronii,
关键词 本研究旨在评估Sanacore ®转基因饲料添加剂对尼罗罗非鱼的生长速度、血液学指标、生化指标、抗氧化谱、非特异性免疫反应以及对维龙单胞菌(A. veronii)的防御能力的影响。将平均初始体重(13.5 ± 1.5 克)为 13.5 ± 1.5 克的两组鱼随机分配为一式三份,分别以 0(对照组)和 5 克/千克的剂量饲喂 Sanacore ® GM 30 天。鱼在实验终点受到蚯蚓甲的挑战,并记录 15 天的死亡率。喂食添加了 Sanacore ® GM 的鱼的最终体重、血液生化指标(红细胞、白细胞、血红蛋白浓度、淋巴细胞、中性粒细胞、血清总蛋白和白蛋白水平)和免疫指标(吞噬细胞活性、一氧化氮、溶菌酶活性、IgM 和血清杀菌活性)均显著增加。此外,抗氧化酶活性(超氧化物歧化酶和谷胱甘肽过氧化物酶)在添加了 Sanacore ® GM 的尼罗罗非鱼日粮中有显著提高。然而,与对照组相比,补充组中过氧化氢酶的活性并不显著(P > 0.05)。与对照组相比,添加 Sanacore ®转基因饲料组的相对存活率为 100%。我们的研究结果表明,Sanacore ®转基因饲料可显著提高尼罗罗非鱼的最终生长率、血液生化指标、免疫反应和抗氧化能力,并增强其对致病性蚯蚓气单胞菌菌株的抗病能力。尼罗罗非鱼
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引用次数: 0
Ameliorative effect of lemon balm on eltroxin induced-hyperthyroidism in rats 柠檬香膏对大鼠甲状腺功能亢进的改善作用
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.275730.1797
Ragaa Salah Kawara, Fatma Moawed, Hussein Abd Elmaksoud, Yakout Elsenosi, Omayma R Abo-Zaid
Keywords Lemon balm has many therapeutic effects including antimicrobial activity (anti-parasitic, antibacterial, antiviral, antifungal, etc.). Various studies have shown that Lemon balm possesses a high amount of antioxidant activity through its chemical components including high amount of flavonoids, rosmaric acid, gallic acid and phenolic contents. This study was designed to evaluate the therapeutic effect of Lemon balm extract against hyperthyroidism induced by Eltroxin. Hyperthyroidism was induced by injecting rats with Eltroxin (100 μg/kg/ day) for 14 days. The hyperthyroid rats were orally treated with Lemon balm extract (75 mg/kg/day) at the beginning of the second week of the Eltroxin injection and continued for another week. The levels of TSH , T3 ,T4 and oxidative biomarkers (NO and CAT ) were measured using commercial kits. Thyroid specimens were taken for histopathological examination. Lemon balm extract reversed the effect of Eltroxin on rats and attenuated the thyroid hormones Additionally, Lemon balm extract alleviated the inflammatory response by suppressing the NO and increasing CAT when compared to hyperthyroid group. Accordingly, these results might strengthen the protective effect of Lemon balm extract in a rat model of hyperthyroidism.
关键词 香茅具有多种治疗效果,包括抗菌活性(抗寄生虫、抗菌、抗病毒、抗真菌等)。多项研究表明,柠檬香膏通过其化学成分(包括大量类黄酮、迷迭香酸、没食子酸和酚类成分)具有很高的抗氧化活性。本研究旨在评估柠檬香膏提取物对艾曲霉素诱导的甲状腺机能亢进症的治疗效果。给大鼠注射甲状腺素(100 μg/kg/天),连续14天,诱发甲亢。在注射甲状腺素的第二周开始,给甲亢大鼠口服香茅提取物(75 毫克/千克/天),并持续一周。使用商业试剂盒测量促甲状腺激素(TSH)、T3、T4 和氧化生物标志物(NO 和 CAT)的水平。采集甲状腺标本进行组织病理学检查。此外,与甲状腺功能亢进组相比,柠檬香脂提取物通过抑制 NO 和增加 CAT 缓解了炎症反应。因此,这些结果可能会加强香茅提取物在甲亢大鼠模型中的保护作用。
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引用次数: 0
Evaluation of the antidiabetic and hepatoprotective effect of Origanum majorana leaf extract and its nanoparticles in streptozotocin-induced diabetes in rats 评估牛至叶提取物及其纳米颗粒在链脲佐菌素诱导的糖尿病大鼠中的抗糖尿病和保肝作用
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.288584.1825
mohamed osama rateb, Afaf D. Abdelmagid, Omnia Abd elhamid, Alshaimaa Said
ABSTRACT
摘要
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引用次数: 0
期刊
Benha Veterinary Medical Journal
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