Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.222598.1680
Pansee Zahid, Enas Soliman, Zeinab Mahdy
Keywords In developing countries, resistance to antimicrobial agents is a serious public health problem, and the indiscriminate use of antimicrobials is a key contributor to the development of resistant bacterial diseases. The following research was aimed to determine the in-vitro antimicrobial activity of (23 strains) of Staphylococcus aureus isolated from 80 random samples of milk and dairy products (soft cheese “Kareish and white” and Roomy cheese) 20 samples from each product, with molecular identification of their resistant genes. The results declared that all isolated strains were totally resistant to Ampicillin (100%), Amoxicillin, and Erythromycin (86.9%), then Oxacillin (65.2%), while all other strains showed intermediate susceptibility to Vancomycin, Cefoxitin, Cefotaxime, and Norfloxacin. While Gentamicin had the highest level of susceptibility (78.3%). Moreover, by the cultivation of the isolated strains on oxacillin resistance screening agar base plates “ORSAB,” there were 15/23 MRSA strains; also, 5/23 strains showed resistance to Vancomycin and grow well in vancomycin screening agar. The molecular identification for the mecA gene and vanA gene was made on seven strains (3 strains resistant to both Oxacillin and Vancomycin, two strains with intermediate resistance, and two strains sensitive to them) isolated from different products (milk, Kariesh cheese, soft white cheese, and Roomy cheese). The results showed that all the samples carried the mecA gene, while only two samples had the vanA gene (isolated from milk). Staphylococcus aureus Antimicrobial resistant mecA gene vanA gene
{"title":"Molecular identification of Methicillin-resistant staphylococcus aureus (MRSA) and Vancomycin- resistant staphylococcus aureus (VRSA) strains isolated from milk and milk products.","authors":"Pansee Zahid, Enas Soliman, Zeinab Mahdy","doi":"10.21608/bvmj.2023.222598.1680","DOIUrl":"https://doi.org/10.21608/bvmj.2023.222598.1680","url":null,"abstract":"Keywords In developing countries, resistance to antimicrobial agents is a serious public health problem, and the indiscriminate use of antimicrobials is a key contributor to the development of resistant bacterial diseases. The following research was aimed to determine the in-vitro antimicrobial activity of (23 strains) of Staphylococcus aureus isolated from 80 random samples of milk and dairy products (soft cheese “Kareish and white” and Roomy cheese) 20 samples from each product, with molecular identification of their resistant genes. The results declared that all isolated strains were totally resistant to Ampicillin (100%), Amoxicillin, and Erythromycin (86.9%), then Oxacillin (65.2%), while all other strains showed intermediate susceptibility to Vancomycin, Cefoxitin, Cefotaxime, and Norfloxacin. While Gentamicin had the highest level of susceptibility (78.3%). Moreover, by the cultivation of the isolated strains on oxacillin resistance screening agar base plates “ORSAB,” there were 15/23 MRSA strains; also, 5/23 strains showed resistance to Vancomycin and grow well in vancomycin screening agar. The molecular identification for the mecA gene and vanA gene was made on seven strains (3 strains resistant to both Oxacillin and Vancomycin, two strains with intermediate resistance, and two strains sensitive to them) isolated from different products (milk, Kariesh cheese, soft white cheese, and Roomy cheese). The results showed that all the samples carried the mecA gene, while only two samples had the vanA gene (isolated from milk). Staphylococcus aureus Antimicrobial resistant mecA gene vanA gene","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Staphylococcus aureus incidence in Egyptian meat outlets and butcheries, and their biofilm, antibiotic-resistance, and virulence capabilities","authors":"Raghda Saad, Rasha Elsabagh, Nahla Abo EL-Roos, shimaa Edris, Islam Sabike","doi":"10.21608/bvmj.2023.224548.1688","DOIUrl":"https://doi.org/10.21608/bvmj.2023.224548.1688","url":null,"abstract":".","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.198161.1644
Eman Mostafa, Eman Sharaf, Fatma Elhofy, Ashraf Abd El Tawab
{"title":"Antibacterial activity of Spirulina Platensis extract against some bacteria as Staphylococcuss aureus, E. coli and Klelbsiella (Invitro) Isolated from Broilers","authors":"Eman Mostafa, Eman Sharaf, Fatma Elhofy, Ashraf Abd El Tawab","doi":"10.21608/bvmj.2023.198161.1644","DOIUrl":"https://doi.org/10.21608/bvmj.2023.198161.1644","url":null,"abstract":"","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.226909.1693
Sara Habk, Mohamed Azab, Randa Ismail, Abir Nafeaa, saad shosha
Keywords The current research aimed to evaluate the effects of L-thyroxine-induced hyperthyroidism and melatonin administration on the histopathology of the thyroid and thymus glands and Bax immunoreactivity. The rats were divided into four groups, comprising ten rats each. Rats were given the following treatments for four weeks: Group I served as the control group; Group II received melatonin; Group III received L-thyroxine; and Group IV received both L-thyroxine and melatonin. The serum levels of total T3 (tT3) and total T4 (tT4) were assessed, as well as histological examinations of the thyroid and thymus and analysis of Bax immunoexpression in both organs. The obtained results showed that in rats with hyperthyroidism, melatonin administration caused a noticeably lower level of thyroid hormones. According to the histopathological examination, hyperthyroidism induced various deteriorating changes in the thyroid and thymus glands, while melatonin therapy reduced these adverse effects. The immunohistochemical analysis demonstrated that hyperthyroid rats displayed a substantial elevation in Bax expression in the thyroid and thymus glands. However, melatonin treatment in hyperthyroid rats resulted in a marked reduction in Bax expression. Thus, it could be concluded that hyperthyroidism induced a range of degenerative changes and markedly elevated Bax expression in thyroid and thymus tissues. However, melatonin intervention ameliorated these negative effects.
{"title":"Cellular Changes in the Thymus Gland induced by Hyperthyroidism and Melatonin Administration","authors":"Sara Habk, Mohamed Azab, Randa Ismail, Abir Nafeaa, saad shosha","doi":"10.21608/bvmj.2023.226909.1693","DOIUrl":"https://doi.org/10.21608/bvmj.2023.226909.1693","url":null,"abstract":"Keywords The current research aimed to evaluate the effects of L-thyroxine-induced hyperthyroidism and melatonin administration on the histopathology of the thyroid and thymus glands and Bax immunoreactivity. The rats were divided into four groups, comprising ten rats each. Rats were given the following treatments for four weeks: Group I served as the control group; Group II received melatonin; Group III received L-thyroxine; and Group IV received both L-thyroxine and melatonin. The serum levels of total T3 (tT3) and total T4 (tT4) were assessed, as well as histological examinations of the thyroid and thymus and analysis of Bax immunoexpression in both organs. The obtained results showed that in rats with hyperthyroidism, melatonin administration caused a noticeably lower level of thyroid hormones. According to the histopathological examination, hyperthyroidism induced various deteriorating changes in the thyroid and thymus glands, while melatonin therapy reduced these adverse effects. The immunohistochemical analysis demonstrated that hyperthyroid rats displayed a substantial elevation in Bax expression in the thyroid and thymus glands. However, melatonin treatment in hyperthyroid rats resulted in a marked reduction in Bax expression. Thus, it could be concluded that hyperthyroidism induced a range of degenerative changes and markedly elevated Bax expression in thyroid and thymus tissues. However, melatonin intervention ameliorated these negative effects.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.222217.1678
dina maria, Elsayed Salim, Afaf Abd El-Magid
Keywords The purpose of this study was to measure the antioxidant state and reactive oxidative stress in the HPG2 cell line in order to explore the effects of Taxol, Atorvastatin, and proanthocynadin. In this study, the effects of Atorvastatin, a novel 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, and Proanthocynadin, the most prevalent polyphenol class in the human diet, on a range of metabolic diseases and chemotherapeutic effects were investigated, Taxol, a chemotherapeutic drug is substance derived from the bark and trunk of the Yew Pacific tree, was shown to assist induce and enhance tubulin polymerization and assembly while also preventing its depolymerization effects on oxidative stress and antioxidant enzymes. Taxol, Atorvastatin, and Proanthcyanidin inhibited the viability of HepG2 cells in a time-and dose-dependent manner, Superoxide dismutase (SOD) and catalase antioxidant enzyme activities were dramatically reduced as a result of the treatment, but glutathione reductase activity, malondialdehyde (MDA) concentrations, and malondialdehyde reductase activity were all significantly higher in treated groups than in untreated cells.
{"title":"The combination of atorvastatin with Proanthocyanidin enhances antioxidant and reactive oxidative stress on HepG2 hepatocellular Carcinoma Cells.","authors":"dina maria, Elsayed Salim, Afaf Abd El-Magid","doi":"10.21608/bvmj.2023.222217.1678","DOIUrl":"https://doi.org/10.21608/bvmj.2023.222217.1678","url":null,"abstract":"Keywords The purpose of this study was to measure the antioxidant state and reactive oxidative stress in the HPG2 cell line in order to explore the effects of Taxol, Atorvastatin, and proanthocynadin. In this study, the effects of Atorvastatin, a novel 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, and Proanthocynadin, the most prevalent polyphenol class in the human diet, on a range of metabolic diseases and chemotherapeutic effects were investigated, Taxol, a chemotherapeutic drug is substance derived from the bark and trunk of the Yew Pacific tree, was shown to assist induce and enhance tubulin polymerization and assembly while also preventing its depolymerization effects on oxidative stress and antioxidant enzymes. Taxol, Atorvastatin, and Proanthcyanidin inhibited the viability of HepG2 cells in a time-and dose-dependent manner, Superoxide dismutase (SOD) and catalase antioxidant enzyme activities were dramatically reduced as a result of the treatment, but glutathione reductase activity, malondialdehyde (MDA) concentrations, and malondialdehyde reductase activity were all significantly higher in treated groups than in untreated cells.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.214177.1667
Nabila Sakr, Amal Abdelnaser, Ibrahim ElBorai, Ayman El-Habbaa
Keywords Enteric diseases represent a major problem in rabbit production. These causes high losses as result of mortalities and decreased conversion indexes. This study focused on viral agents (e.g., Astrovirus, Coronavirus (CoV), Rotavirus (RV) and Rabbit Hemorrhagic Disease Virus (RHDV)) as one of the causes of diarrhea in rabbits. 140 samples were collected from commercial farms located at Menofia and Qalyubia governorates, Egypt, during the period from October 2020 to May 2021. Our study focused on live and dead rabbits who showed diarrhea at the age of 7 days and up to 150 days of age. The most apparent symptoms were diarrhea, and the postmortem examination showed the presence of localized area of necrosis in different organs and splenomegaly. All samples in the current research came up negative for Astrovirus, Corona virus and Rota virus using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and though some samples were positive for RHDV in the liver and intestinal samples of dead rabbits with enterocolitis. Sequencing and phylogenetic analysis of the VP60 gene of the RHDV strain showed that it is related to RHDV2. Genetic analysis of our strain (RHV-Egypt-BenhaF954-2022) was clustered with G1.2/RHDV2/b strains with homology 97.1%. Homology with Egyptian classical strains were 96.6% with G1.1C/RHDV and 87.6% with G1.1a/RHDVa. The genetic diversity between the study obtained strain and other reference RHDV strain is indicative to probability of antigenic diversity.
{"title":"Molecular detection of Viral diarrhea in Rabbits","authors":"Nabila Sakr, Amal Abdelnaser, Ibrahim ElBorai, Ayman El-Habbaa","doi":"10.21608/bvmj.2023.214177.1667","DOIUrl":"https://doi.org/10.21608/bvmj.2023.214177.1667","url":null,"abstract":"Keywords Enteric diseases represent a major problem in rabbit production. These causes high losses as result of mortalities and decreased conversion indexes. This study focused on viral agents (e.g., Astrovirus, Coronavirus (CoV), Rotavirus (RV) and Rabbit Hemorrhagic Disease Virus (RHDV)) as one of the causes of diarrhea in rabbits. 140 samples were collected from commercial farms located at Menofia and Qalyubia governorates, Egypt, during the period from October 2020 to May 2021. Our study focused on live and dead rabbits who showed diarrhea at the age of 7 days and up to 150 days of age. The most apparent symptoms were diarrhea, and the postmortem examination showed the presence of localized area of necrosis in different organs and splenomegaly. All samples in the current research came up negative for Astrovirus, Corona virus and Rota virus using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and though some samples were positive for RHDV in the liver and intestinal samples of dead rabbits with enterocolitis. Sequencing and phylogenetic analysis of the VP60 gene of the RHDV strain showed that it is related to RHDV2. Genetic analysis of our strain (RHV-Egypt-BenhaF954-2022) was clustered with G1.2/RHDV2/b strains with homology 97.1%. Homology with Egyptian classical strains were 96.6% with G1.1C/RHDV and 87.6% with G1.1a/RHDVa. The genetic diversity between the study obtained strain and other reference RHDV strain is indicative to probability of antigenic diversity.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"209 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.220924.1675
Manar Sallam, Nashwa Khalifa, Ashraf Barakat, Lobna Salem
{"title":"Occurrence of Staphylococcus aureus in dairy farms and humans with reference to antimicrobial profile in Qalyobia Governorate","authors":"Manar Sallam, Nashwa Khalifa, Ashraf Barakat, Lobna Salem","doi":"10.21608/bvmj.2023.220924.1675","DOIUrl":"https://doi.org/10.21608/bvmj.2023.220924.1675","url":null,"abstract":"","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.225439.1691
Karam Hakeem, Fahim Shaltout, Eman El-Diasty
Keywords During storage, the fish product can absorb moisture from its surroundings, promoting the development of microorganisms. During storage, the fish product can absorb moisture from its surroundings, promoting the development of microorganisms. Thus, the effects of thyme, marjoram , turmeric extracts and their combinations on microbiological profile, chemical parameters (pH, TVN and TBA) as well as organoleptic traits were studied during refrigerated storage (0 ± 1 0 C) over a period of 12 days. Nile tilapia fish fillets were divided into 7 groups; according to plant extracts alone or combined. The first group was (T1) control; T2 majourium; T3 thyme; T4 majourium + thyme; T5 turmeric; T6 turmeric + majourium; and T7 turmeric + thyme (1.5%v/w from each extract). The obtained results indicated that the turmeric alone and combined treatment of plant extracts of marjoram, thyme, and turmeric slowed up the microbial growth and be late the chemical changes, kept the sensory attributes, and enhanced the shelf life of the Nile tilapia fish fillet during refrigerated storage. Moreover, the results proved that the control samples were unacceptable due to spoilage on the 6th day of storage. The plant extracts can inhibit the microbial growth and improve the chemical and sensory attributes and subsequently enhance the shelf life of such examined samples during refrigerated
{"title":"Effects of natural compounds of some plants on microbial contamination and sensory quality of fish fillet during refrigeration","authors":"Karam Hakeem, Fahim Shaltout, Eman El-Diasty","doi":"10.21608/bvmj.2023.225439.1691","DOIUrl":"https://doi.org/10.21608/bvmj.2023.225439.1691","url":null,"abstract":"Keywords During storage, the fish product can absorb moisture from its surroundings, promoting the development of microorganisms. During storage, the fish product can absorb moisture from its surroundings, promoting the development of microorganisms. Thus, the effects of thyme, marjoram , turmeric extracts and their combinations on microbiological profile, chemical parameters (pH, TVN and TBA) as well as organoleptic traits were studied during refrigerated storage (0 ± 1 0 C) over a period of 12 days. Nile tilapia fish fillets were divided into 7 groups; according to plant extracts alone or combined. The first group was (T1) control; T2 majourium; T3 thyme; T4 majourium + thyme; T5 turmeric; T6 turmeric + majourium; and T7 turmeric + thyme (1.5%v/w from each extract). The obtained results indicated that the turmeric alone and combined treatment of plant extracts of marjoram, thyme, and turmeric slowed up the microbial growth and be late the chemical changes, kept the sensory attributes, and enhanced the shelf life of the Nile tilapia fish fillet during refrigerated storage. Moreover, the results proved that the control samples were unacceptable due to spoilage on the 6th day of storage. The plant extracts can inhibit the microbial growth and improve the chemical and sensory attributes and subsequently enhance the shelf life of such examined samples during refrigerated","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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