Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.222598.1680
Pansee Zahid, Enas Soliman, Zeinab Mahdy
Keywords In developing countries, resistance to antimicrobial agents is a serious public health problem, and the indiscriminate use of antimicrobials is a key contributor to the development of resistant bacterial diseases. The following research was aimed to determine the in-vitro antimicrobial activity of (23 strains) of Staphylococcus aureus isolated from 80 random samples of milk and dairy products (soft cheese “Kareish and white” and Roomy cheese) 20 samples from each product, with molecular identification of their resistant genes. The results declared that all isolated strains were totally resistant to Ampicillin (100%), Amoxicillin, and Erythromycin (86.9%), then Oxacillin (65.2%), while all other strains showed intermediate susceptibility to Vancomycin, Cefoxitin, Cefotaxime, and Norfloxacin. While Gentamicin had the highest level of susceptibility (78.3%). Moreover, by the cultivation of the isolated strains on oxacillin resistance screening agar base plates “ORSAB,” there were 15/23 MRSA strains; also, 5/23 strains showed resistance to Vancomycin and grow well in vancomycin screening agar. The molecular identification for the mecA gene and vanA gene was made on seven strains (3 strains resistant to both Oxacillin and Vancomycin, two strains with intermediate resistance, and two strains sensitive to them) isolated from different products (milk, Kariesh cheese, soft white cheese, and Roomy cheese). The results showed that all the samples carried the mecA gene, while only two samples had the vanA gene (isolated from milk). Staphylococcus aureus Antimicrobial resistant mecA gene vanA gene
{"title":"Molecular identification of Methicillin-resistant staphylococcus aureus (MRSA) and Vancomycin- resistant staphylococcus aureus (VRSA) strains isolated from milk and milk products.","authors":"Pansee Zahid, Enas Soliman, Zeinab Mahdy","doi":"10.21608/bvmj.2023.222598.1680","DOIUrl":"https://doi.org/10.21608/bvmj.2023.222598.1680","url":null,"abstract":"Keywords In developing countries, resistance to antimicrobial agents is a serious public health problem, and the indiscriminate use of antimicrobials is a key contributor to the development of resistant bacterial diseases. The following research was aimed to determine the in-vitro antimicrobial activity of (23 strains) of Staphylococcus aureus isolated from 80 random samples of milk and dairy products (soft cheese “Kareish and white” and Roomy cheese) 20 samples from each product, with molecular identification of their resistant genes. The results declared that all isolated strains were totally resistant to Ampicillin (100%), Amoxicillin, and Erythromycin (86.9%), then Oxacillin (65.2%), while all other strains showed intermediate susceptibility to Vancomycin, Cefoxitin, Cefotaxime, and Norfloxacin. While Gentamicin had the highest level of susceptibility (78.3%). Moreover, by the cultivation of the isolated strains on oxacillin resistance screening agar base plates “ORSAB,” there were 15/23 MRSA strains; also, 5/23 strains showed resistance to Vancomycin and grow well in vancomycin screening agar. The molecular identification for the mecA gene and vanA gene was made on seven strains (3 strains resistant to both Oxacillin and Vancomycin, two strains with intermediate resistance, and two strains sensitive to them) isolated from different products (milk, Kariesh cheese, soft white cheese, and Roomy cheese). The results showed that all the samples carried the mecA gene, while only two samples had the vanA gene (isolated from milk). Staphylococcus aureus Antimicrobial resistant mecA gene vanA gene","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Staphylococcus aureus incidence in Egyptian meat outlets and butcheries, and their biofilm, antibiotic-resistance, and virulence capabilities","authors":"Raghda Saad, Rasha Elsabagh, Nahla Abo EL-Roos, shimaa Edris, Islam Sabike","doi":"10.21608/bvmj.2023.224548.1688","DOIUrl":"https://doi.org/10.21608/bvmj.2023.224548.1688","url":null,"abstract":".","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.198161.1644
Eman Mostafa, Eman Sharaf, Fatma Elhofy, Ashraf Abd El Tawab
{"title":"Antibacterial activity of Spirulina Platensis extract against some bacteria as Staphylococcuss aureus, E. coli and Klelbsiella (Invitro) Isolated from Broilers","authors":"Eman Mostafa, Eman Sharaf, Fatma Elhofy, Ashraf Abd El Tawab","doi":"10.21608/bvmj.2023.198161.1644","DOIUrl":"https://doi.org/10.21608/bvmj.2023.198161.1644","url":null,"abstract":"","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.214177.1667
Nabila Sakr, Amal Abdelnaser, Ibrahim ElBorai, Ayman El-Habbaa
Keywords Enteric diseases represent a major problem in rabbit production. These causes high losses as result of mortalities and decreased conversion indexes. This study focused on viral agents (e.g., Astrovirus, Coronavirus (CoV), Rotavirus (RV) and Rabbit Hemorrhagic Disease Virus (RHDV)) as one of the causes of diarrhea in rabbits. 140 samples were collected from commercial farms located at Menofia and Qalyubia governorates, Egypt, during the period from October 2020 to May 2021. Our study focused on live and dead rabbits who showed diarrhea at the age of 7 days and up to 150 days of age. The most apparent symptoms were diarrhea, and the postmortem examination showed the presence of localized area of necrosis in different organs and splenomegaly. All samples in the current research came up negative for Astrovirus, Corona virus and Rota virus using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and though some samples were positive for RHDV in the liver and intestinal samples of dead rabbits with enterocolitis. Sequencing and phylogenetic analysis of the VP60 gene of the RHDV strain showed that it is related to RHDV2. Genetic analysis of our strain (RHV-Egypt-BenhaF954-2022) was clustered with G1.2/RHDV2/b strains with homology 97.1%. Homology with Egyptian classical strains were 96.6% with G1.1C/RHDV and 87.6% with G1.1a/RHDVa. The genetic diversity between the study obtained strain and other reference RHDV strain is indicative to probability of antigenic diversity.
{"title":"Molecular detection of Viral diarrhea in Rabbits","authors":"Nabila Sakr, Amal Abdelnaser, Ibrahim ElBorai, Ayman El-Habbaa","doi":"10.21608/bvmj.2023.214177.1667","DOIUrl":"https://doi.org/10.21608/bvmj.2023.214177.1667","url":null,"abstract":"Keywords Enteric diseases represent a major problem in rabbit production. These causes high losses as result of mortalities and decreased conversion indexes. This study focused on viral agents (e.g., Astrovirus, Coronavirus (CoV), Rotavirus (RV) and Rabbit Hemorrhagic Disease Virus (RHDV)) as one of the causes of diarrhea in rabbits. 140 samples were collected from commercial farms located at Menofia and Qalyubia governorates, Egypt, during the period from October 2020 to May 2021. Our study focused on live and dead rabbits who showed diarrhea at the age of 7 days and up to 150 days of age. The most apparent symptoms were diarrhea, and the postmortem examination showed the presence of localized area of necrosis in different organs and splenomegaly. All samples in the current research came up negative for Astrovirus, Corona virus and Rota virus using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and though some samples were positive for RHDV in the liver and intestinal samples of dead rabbits with enterocolitis. Sequencing and phylogenetic analysis of the VP60 gene of the RHDV strain showed that it is related to RHDV2. Genetic analysis of our strain (RHV-Egypt-BenhaF954-2022) was clustered with G1.2/RHDV2/b strains with homology 97.1%. Homology with Egyptian classical strains were 96.6% with G1.1C/RHDV and 87.6% with G1.1a/RHDVa. The genetic diversity between the study obtained strain and other reference RHDV strain is indicative to probability of antigenic diversity.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"209 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.220924.1675
Manar Sallam, Nashwa Khalifa, Ashraf Barakat, Lobna Salem
{"title":"Occurrence of Staphylococcus aureus in dairy farms and humans with reference to antimicrobial profile in Qalyobia Governorate","authors":"Manar Sallam, Nashwa Khalifa, Ashraf Barakat, Lobna Salem","doi":"10.21608/bvmj.2023.220924.1675","DOIUrl":"https://doi.org/10.21608/bvmj.2023.220924.1675","url":null,"abstract":"","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.225439.1691
Karam Hakeem, Fahim Shaltout, Eman El-Diasty
Keywords During storage, the fish product can absorb moisture from its surroundings, promoting the development of microorganisms. During storage, the fish product can absorb moisture from its surroundings, promoting the development of microorganisms. Thus, the effects of thyme, marjoram , turmeric extracts and their combinations on microbiological profile, chemical parameters (pH, TVN and TBA) as well as organoleptic traits were studied during refrigerated storage (0 ± 1 0 C) over a period of 12 days. Nile tilapia fish fillets were divided into 7 groups; according to plant extracts alone or combined. The first group was (T1) control; T2 majourium; T3 thyme; T4 majourium + thyme; T5 turmeric; T6 turmeric + majourium; and T7 turmeric + thyme (1.5%v/w from each extract). The obtained results indicated that the turmeric alone and combined treatment of plant extracts of marjoram, thyme, and turmeric slowed up the microbial growth and be late the chemical changes, kept the sensory attributes, and enhanced the shelf life of the Nile tilapia fish fillet during refrigerated storage. Moreover, the results proved that the control samples were unacceptable due to spoilage on the 6th day of storage. The plant extracts can inhibit the microbial growth and improve the chemical and sensory attributes and subsequently enhance the shelf life of such examined samples during refrigerated
{"title":"Effects of natural compounds of some plants on microbial contamination and sensory quality of fish fillet during refrigeration","authors":"Karam Hakeem, Fahim Shaltout, Eman El-Diasty","doi":"10.21608/bvmj.2023.225439.1691","DOIUrl":"https://doi.org/10.21608/bvmj.2023.225439.1691","url":null,"abstract":"Keywords During storage, the fish product can absorb moisture from its surroundings, promoting the development of microorganisms. During storage, the fish product can absorb moisture from its surroundings, promoting the development of microorganisms. Thus, the effects of thyme, marjoram , turmeric extracts and their combinations on microbiological profile, chemical parameters (pH, TVN and TBA) as well as organoleptic traits were studied during refrigerated storage (0 ± 1 0 C) over a period of 12 days. Nile tilapia fish fillets were divided into 7 groups; according to plant extracts alone or combined. The first group was (T1) control; T2 majourium; T3 thyme; T4 majourium + thyme; T5 turmeric; T6 turmeric + majourium; and T7 turmeric + thyme (1.5%v/w from each extract). The obtained results indicated that the turmeric alone and combined treatment of plant extracts of marjoram, thyme, and turmeric slowed up the microbial growth and be late the chemical changes, kept the sensory attributes, and enhanced the shelf life of the Nile tilapia fish fillet during refrigerated storage. Moreover, the results proved that the control samples were unacceptable due to spoilage on the 6th day of storage. The plant extracts can inhibit the microbial growth and improve the chemical and sensory attributes and subsequently enhance the shelf life of such examined samples during refrigerated","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The potential contribution of food contact surfaces to the incidence of Escherichia coli in Egyptian meat outlets, as well as their biofilm and pathogenicity characteristics.","authors":"Raghda Saad, Islam Sabike, Nahla Abo EL-Roos, shimaa Edris, Rasha Elsabagh","doi":"10.21608/bvmj.2023.225650.1694","DOIUrl":"https://doi.org/10.21608/bvmj.2023.225650.1694","url":null,"abstract":"","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.228158.1700
Dina Awad, Osama Ahmed, Samar Baloza, Maha Mamdouh
Keywords Dromedary milk has a proven outstanding history of being a nutrient-rich food. Camel milk possesses both medicinal and technological advantages, including antioxidant, antibacterial, and antiparasitic activities. The present investigation is proposed to assess the antibacterial ability and free radical scavenging capacity against Gram-negative bacteria of various enzyme-digested skimmed camel’s milk (SCM) using papain, pepsin, and trypsin at four hydrolysis times at one-hour intervals. An antibacterial growth assay was conducted to detect the antibacterial capacity of the generated various enzymatic camel milk hydrolysates. The results showed that the antibacterial activity of unhydrolyzed intact SCM displayed mild effect against Escherichia coli and no effect against Salmonella enteritidis . Pepsin-treated SCM hydrolysates after 3h hydrolysis significantly enhanced SCM antimicrobial activity against E. coli , and after 2h, hydrolysis produces SCM hydrolysates against Salmonella enteritidis. Pepsin and trypsin SCM-treated hydrolysates after 2h hydrolysis could completely abolish the survival growth rate of Salmonella enteritidis. Additionally, hydrolysates treated with papain SCM after 3 and 4 hours of hydrolysis showed a 100% ability to kill Salmonella enteritidis . Both DPPH method and ABTS assays were employed to evaluate antioxidants. Our findings demonstrated that all SCM hydrolysates gradually acquired strong antioxidant capacity after four hours of hydrolysis, with trypsin-treated hydrolysates having the highest values. The current work offered a great role of skimmed camel’s milk hydrolysates and the possibilities for using these hydrolysates as food natural preservatives.
{"title":"Dual functional properties of enzymatically hydrolyzed dromedary milk proteins with improved antioxidant and antibacterial activity","authors":"Dina Awad, Osama Ahmed, Samar Baloza, Maha Mamdouh","doi":"10.21608/bvmj.2023.228158.1700","DOIUrl":"https://doi.org/10.21608/bvmj.2023.228158.1700","url":null,"abstract":"Keywords Dromedary milk has a proven outstanding history of being a nutrient-rich food. Camel milk possesses both medicinal and technological advantages, including antioxidant, antibacterial, and antiparasitic activities. The present investigation is proposed to assess the antibacterial ability and free radical scavenging capacity against Gram-negative bacteria of various enzyme-digested skimmed camel’s milk (SCM) using papain, pepsin, and trypsin at four hydrolysis times at one-hour intervals. An antibacterial growth assay was conducted to detect the antibacterial capacity of the generated various enzymatic camel milk hydrolysates. The results showed that the antibacterial activity of unhydrolyzed intact SCM displayed mild effect against Escherichia coli and no effect against Salmonella enteritidis . Pepsin-treated SCM hydrolysates after 3h hydrolysis significantly enhanced SCM antimicrobial activity against E. coli , and after 2h, hydrolysis produces SCM hydrolysates against Salmonella enteritidis. Pepsin and trypsin SCM-treated hydrolysates after 2h hydrolysis could completely abolish the survival growth rate of Salmonella enteritidis. Additionally, hydrolysates treated with papain SCM after 3 and 4 hours of hydrolysis showed a 100% ability to kill Salmonella enteritidis . Both DPPH method and ABTS assays were employed to evaluate antioxidants. Our findings demonstrated that all SCM hydrolysates gradually acquired strong antioxidant capacity after four hours of hydrolysis, with trypsin-treated hydrolysates having the highest values. The current work offered a great role of skimmed camel’s milk hydrolysates and the possibilities for using these hydrolysates as food natural preservatives.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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