Keywords FMD is an important viral disease that affects animals with cloven hooves. Inactivated oil adjuvant FMD vaccines are widely used in prophylactic vaccination programs for protection of animals and for safe and effective immunization. In this study, we prepared a bivalent inactivated vaccine form recently isolated FMDV strains A/Africa G4/2022 and A/ Venezuela/2022 serotypes using different ratio of Ictyolane 18 oil adjuvant and evaluate quality and immunogenicity safety of vaccine. Laboratory evaluation of the vaccine using calves that vaccinated intramuscularly (I/M) with the prepared bivalent FMD vaccines (group 1 using the vaccine emulsion 50 % Ictyolane 18 oil with 50% antigen and group 2 using the vaccine emulsion 60 % Ictyolane 18 with 40% antigen). Serum samples collected from animals that had received vaccination were nine months. Serum Neutralization Test (SNT) and the ELISA method were used to monitor the humeral immune responses. The findings showed that the protective level was reached by vaccinated calves at the second to third week following vaccination (WPV) and persisted up to nine months following vaccination. The findings demonstrate that the inclusion of the two ratio of Ictyolane 18 in Cattle receive an early and prolonged high-specific protective immune response from the FMDV vaccine. Finally, we recommended to use Ictyolane 18 as a potential safe and very cost-effective adjuvant with an equal ratio of FMD antigen for the cattle FMD vaccination.
{"title":"The efficacy of FMD vaccine prepared from recently isolated FMD virus using Ictyolane 18 oil adjuvant","authors":"Hadeer Amin, Gabr El-Bagoury, Ayman El-Habbaa, hiam fakhry","doi":"10.21608/bvmj.2023.217414.1671","DOIUrl":"https://doi.org/10.21608/bvmj.2023.217414.1671","url":null,"abstract":"Keywords FMD is an important viral disease that affects animals with cloven hooves. Inactivated oil adjuvant FMD vaccines are widely used in prophylactic vaccination programs for protection of animals and for safe and effective immunization. In this study, we prepared a bivalent inactivated vaccine form recently isolated FMDV strains A/Africa G4/2022 and A/ Venezuela/2022 serotypes using different ratio of Ictyolane 18 oil adjuvant and evaluate quality and immunogenicity safety of vaccine. Laboratory evaluation of the vaccine using calves that vaccinated intramuscularly (I/M) with the prepared bivalent FMD vaccines (group 1 using the vaccine emulsion 50 % Ictyolane 18 oil with 50% antigen and group 2 using the vaccine emulsion 60 % Ictyolane 18 with 40% antigen). Serum samples collected from animals that had received vaccination were nine months. Serum Neutralization Test (SNT) and the ELISA method were used to monitor the humeral immune responses. The findings showed that the protective level was reached by vaccinated calves at the second to third week following vaccination (WPV) and persisted up to nine months following vaccination. The findings demonstrate that the inclusion of the two ratio of Ictyolane 18 in Cattle receive an early and prolonged high-specific protective immune response from the FMDV vaccine. Finally, we recommended to use Ictyolane 18 as a potential safe and very cost-effective adjuvant with an equal ratio of FMD antigen for the cattle FMD vaccination.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.223470.1683
Mohamed Ramadan, Fatma Elhofy, Ashraf Abd El Tawab
Keywords The purpose of this study is to assess the accuracy of the MALDI TOF technique in identifying Klebsiella spp. Plus, application of MICRONAUT susceptibility testing for detection of MDR klebsiella isolates. A total of 293 samples (233 nasal swabs, 45 milk sample, 10 anal swabs and 5 vaginal swabs ) were collected from 258 clinically diseased animals. All samples were examined and all typical colonies were analyzed biochemically and confirmed by MALDI TOF method. All confirmed klebislla spp. isolates were tested for antimicrobial susceptibility to 18 antimicrobials. Typical colonies were confirmed biochemically in 30 samples as klebsiella (10.2%). They were identified as klebsiella spp. (n=24), k. pneumoniae (n=3) and enterobacter spp. (n=3) by MALDI TOF. Antibiotic susceptibility testing of 27 k. pneumoniea strains by using MICRONAUT susceptibility testing discovered that all of the isolated strains were sensitive to 7/16 tested antibiotics (43.75%) amoxicillin/clavulanic acid, ceftiofur, cephalothin, enrofloxacin, gentamicin, spectinomycin, trimethoprim/sulfamethoxazol, complete resistance was observed to only 6 antibiotics, i.e., ampicillin erythromycin , tiamulin, tilmicosin, penicillin G, tulathromycin. All strains showed intermediate susceptible to florfenicol except only one strain. Eighty five percent of the isolates recorded sensitivity to colistin and the remaining show intermediate susceptibility. Ten strains were unsusceptible to tetracycline (6 resistance, 4 intermediate) and the other were susceptible to tetracycline.
{"title":"MALDI-TOF MS and MICRONAUT susceptibility test as recent bacteriological applications of Klebsiella spp isolated from large animals in Egypt.","authors":"Mohamed Ramadan, Fatma Elhofy, Ashraf Abd El Tawab","doi":"10.21608/bvmj.2023.223470.1683","DOIUrl":"https://doi.org/10.21608/bvmj.2023.223470.1683","url":null,"abstract":"Keywords The purpose of this study is to assess the accuracy of the MALDI TOF technique in identifying Klebsiella spp. Plus, application of MICRONAUT susceptibility testing for detection of MDR klebsiella isolates. A total of 293 samples (233 nasal swabs, 45 milk sample, 10 anal swabs and 5 vaginal swabs ) were collected from 258 clinically diseased animals. All samples were examined and all typical colonies were analyzed biochemically and confirmed by MALDI TOF method. All confirmed klebislla spp. isolates were tested for antimicrobial susceptibility to 18 antimicrobials. Typical colonies were confirmed biochemically in 30 samples as klebsiella (10.2%). They were identified as klebsiella spp. (n=24), k. pneumoniae (n=3) and enterobacter spp. (n=3) by MALDI TOF. Antibiotic susceptibility testing of 27 k. pneumoniea strains by using MICRONAUT susceptibility testing discovered that all of the isolated strains were sensitive to 7/16 tested antibiotics (43.75%) amoxicillin/clavulanic acid, ceftiofur, cephalothin, enrofloxacin, gentamicin, spectinomycin, trimethoprim/sulfamethoxazol, complete resistance was observed to only 6 antibiotics, i.e., ampicillin erythromycin , tiamulin, tilmicosin, penicillin G, tulathromycin. All strains showed intermediate susceptible to florfenicol except only one strain. Eighty five percent of the isolates recorded sensitivity to colistin and the remaining show intermediate susceptibility. Ten strains were unsusceptible to tetracycline (6 resistance, 4 intermediate) and the other were susceptible to tetracycline.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.216116.1669
Ibrahim Abdelwadoud, Afaf eldesoky, Elsayed Salem
Keywords The present study aimed to evaluate the positive effects of amygdalin against mammalian tumors in vivo . Twenty female albino mice, 4-6 weeks old age, were equally divided into four groups: group I (control) received no drugs; group II (carcinogenic group) received oral 7,12-dimethylbenz[a]anthracene (DMBA) (50 mg/kg b.wt. once a weak) dissolved in sesame oil for 4 weeks; group III (treatment group) received oral amygdalin (0.6 mg/kg b.wt./day) dissolved in 100% corn oil for 4 weeks after DMBA-induced tumor as in group II; group IV (protection group) received amygdalin as in group III prior DMBA administration. The results showed that DMBA-induced mammalian tumors caused a significant increase in serum bilirubin (total and direct). Still, a substantial decrease in serum albumin as a hepatic function was recorded. Serum uric acid as a kidney marker revealed an insignificant increase. In addition, mammalian tissue L-malondialdehyde (L-MDA) concentration showed substantial and a considerable decrease in mammalian tissue total antioxidant capacity (TAC) concentration compared with the control group. However, the administration of amygdalin was able to mitigate DMBA-induced mammalian tumors through decreasing total and direct bilirubin, uric acid concentration, as well as L-MDA concentration, along with a significant increase of TAC concentration in mammalian tissue. Additionally, hematological parameters of amygdalin-administrated mice showed a substantial increase compared to mice with mammalian tumors induced by DMBA. Thus, it can be concluded that amygdalin may successfully protect mammary tumors.
{"title":"In vivo study using Amygdalin against mammalian tumors in a mice model","authors":"Ibrahim Abdelwadoud, Afaf eldesoky, Elsayed Salem","doi":"10.21608/bvmj.2023.216116.1669","DOIUrl":"https://doi.org/10.21608/bvmj.2023.216116.1669","url":null,"abstract":"Keywords The present study aimed to evaluate the positive effects of amygdalin against mammalian tumors in vivo . Twenty female albino mice, 4-6 weeks old age, were equally divided into four groups: group I (control) received no drugs; group II (carcinogenic group) received oral 7,12-dimethylbenz[a]anthracene (DMBA) (50 mg/kg b.wt. once a weak) dissolved in sesame oil for 4 weeks; group III (treatment group) received oral amygdalin (0.6 mg/kg b.wt./day) dissolved in 100% corn oil for 4 weeks after DMBA-induced tumor as in group II; group IV (protection group) received amygdalin as in group III prior DMBA administration. The results showed that DMBA-induced mammalian tumors caused a significant increase in serum bilirubin (total and direct). Still, a substantial decrease in serum albumin as a hepatic function was recorded. Serum uric acid as a kidney marker revealed an insignificant increase. In addition, mammalian tissue L-malondialdehyde (L-MDA) concentration showed substantial and a considerable decrease in mammalian tissue total antioxidant capacity (TAC) concentration compared with the control group. However, the administration of amygdalin was able to mitigate DMBA-induced mammalian tumors through decreasing total and direct bilirubin, uric acid concentration, as well as L-MDA concentration, along with a significant increase of TAC concentration in mammalian tissue. Additionally, hematological parameters of amygdalin-administrated mice showed a substantial increase compared to mice with mammalian tumors induced by DMBA. Thus, it can be concluded that amygdalin may successfully protect mammary tumors.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.204239.1650
Hytham mosaad, Hemmat Ibrahim, Mohamed Hassan, Amina Elrais, Walid Arab
.
{"title":"Hygienic evaluation of Meat Handlers and equipment in meat preparation Sector in some markets in Qalyubia Governorate","authors":"Hytham mosaad, Hemmat Ibrahim, Mohamed Hassan, Amina Elrais, Walid Arab","doi":"10.21608/bvmj.2023.204239.1650","DOIUrl":"https://doi.org/10.21608/bvmj.2023.204239.1650","url":null,"abstract":".","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.210564.1660
Mohamed Ali, Hiam Elabd, Mohsen Azouz, Adel Shaheen, Amel El Asely, Eman Abd El-Gawad, Aya Matter, Hadeer Youssuf, Amany Abbass
.
{"title":"Effects of prebiotics supplementation on Nile tilapia, emphasizing phospholipids aquafeed - A review","authors":"Mohamed Ali, Hiam Elabd, Mohsen Azouz, Adel Shaheen, Amel El Asely, Eman Abd El-Gawad, Aya Matter, Hadeer Youssuf, Amany Abbass","doi":"10.21608/bvmj.2023.210564.1660","DOIUrl":"https://doi.org/10.21608/bvmj.2023.210564.1660","url":null,"abstract":".","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.229790.1709
Aya Farouk, Amany Salem, Marionette Nassif
{"title":"Seasonal Impact on the Microbiological Quality of Some Poultry Meat cuts","authors":"Aya Farouk, Amany Salem, Marionette Nassif","doi":"10.21608/bvmj.2023.229790.1709","DOIUrl":"https://doi.org/10.21608/bvmj.2023.229790.1709","url":null,"abstract":"","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.235050.1719
Aya Farouk, Amany Salem, Marionette Nassif
{"title":"Tracking the antibiotic resistance phenomenon in poultry meats under species and seasonal variations","authors":"Aya Farouk, Amany Salem, Marionette Nassif","doi":"10.21608/bvmj.2023.235050.1719","DOIUrl":"https://doi.org/10.21608/bvmj.2023.235050.1719","url":null,"abstract":"","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.227338.1696
Rasha Deiab, Nahla Abo Elroos, Ashraf Abd El Tawab
Keywords Pseudomonas is a genus of Gram-negative bacteria belonging to the family Pseudomonadaceae. It is one of the psychrophilic bacteria that can thrive in low-temperature milk in addition to producing heat-resistant spoiling enzymes due to the presence of virulence genes. So, the aim of this study is to characterize the virulence potential of Pseudomonas species isolated from bulk tank milk. Four strains of pseudomonas species were identified as Pseudomonas aeruginosa, Pseudomonas fluorescence, Pseudomonas putida, and Pseudomonas diminuta in the percentages of 16, 12, 4 and 4 (%,) respectively. The pathogenesis of Pseudomonas species is closely related to its virulence genes. In the present study, the virulence genes that were identified in the isolated samples were outer membrane lipoprotein L (oprL) and exotoxin S (exoS) virulence genes which detected in all studied strains, while exo polysaccharide synthesis locus (pslA) virulence gene was detected in 80% of the studied strains, on the other hand exotoxin A (toxA) virulence gene was detected in 70% of the studied strains. Moreover, the elastase B (lasB) virulence gene was detected in 20% of the studied strains.
{"title":"Molecular Characterization of Virulence genes in Pseudomonas Species Isolated from Bulk tank Milk in El Menofia Governorate","authors":"Rasha Deiab, Nahla Abo Elroos, Ashraf Abd El Tawab","doi":"10.21608/bvmj.2023.227338.1696","DOIUrl":"https://doi.org/10.21608/bvmj.2023.227338.1696","url":null,"abstract":"Keywords Pseudomonas is a genus of Gram-negative bacteria belonging to the family Pseudomonadaceae. It is one of the psychrophilic bacteria that can thrive in low-temperature milk in addition to producing heat-resistant spoiling enzymes due to the presence of virulence genes. So, the aim of this study is to characterize the virulence potential of Pseudomonas species isolated from bulk tank milk. Four strains of pseudomonas species were identified as Pseudomonas aeruginosa, Pseudomonas fluorescence, Pseudomonas putida, and Pseudomonas diminuta in the percentages of 16, 12, 4 and 4 (%,) respectively. The pathogenesis of Pseudomonas species is closely related to its virulence genes. In the present study, the virulence genes that were identified in the isolated samples were outer membrane lipoprotein L (oprL) and exotoxin S (exoS) virulence genes which detected in all studied strains, while exo polysaccharide synthesis locus (pslA) virulence gene was detected in 80% of the studied strains, on the other hand exotoxin A (toxA) virulence gene was detected in 70% of the studied strains. Moreover, the elastase B (lasB) virulence gene was detected in 20% of the studied strains.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.230689.1711
Abdelhaleem elkasapy
ABSTRACT
{"title":"The influence of the different cherry eye surgical intervention on tear production in dogs","authors":"Abdelhaleem elkasapy","doi":"10.21608/bvmj.2023.230689.1711","DOIUrl":"https://doi.org/10.21608/bvmj.2023.230689.1711","url":null,"abstract":"ABSTRACT","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/bvmj.2023.227369.1697
Rasha Deiab, Nahla Abo El- Roos, Ashraf Awad
ABSTRACT
{"title":"Biofilm production by pseudomonas species isolated from bulk tank milk and some milk products","authors":"Rasha Deiab, Nahla Abo El- Roos, Ashraf Awad","doi":"10.21608/bvmj.2023.227369.1697","DOIUrl":"https://doi.org/10.21608/bvmj.2023.227369.1697","url":null,"abstract":"ABSTRACT","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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