M. Tofangchiha, F. Arianfar, M. Bakhshi, M. Khorasani
Background: Cone beam computed tomography (CT) has recently become effective for oral and maxillofacial imaging. Objectives: The aim of this study was to assess the knowledge of dentists regarding cone beam computed tomography. Materials and Methods: In this descriptive cross sectional study, a questionnaire regarding cone-beam computed tomography (CBCT) was distributed amongst 100 dentists (general and specialist) in Qazvin, Iran. Their level of knowledge was compared in each section on the basis of age, gender, years of employment and last educational status and analyzed by the SPSS software and Mann-Whitney test. Results: Data analysis showed that 4% of dentists had very low, 16% had low, 50% had medium, 19% had good and 11% had in very good level of knowledge. The average of dentists’ knowledge was 57 ± 18. According to the statistical results, there was a significant difference between level of knowledge and age, years of employment and educational degree (P 0.05). Conclusions: Overall, dentists had an average level of knowledge for CBCT. It is recommended for qualification programs to be held for dentists to strengthen their awareness toward cone beam computed tomography. Keywords:Knowledge; Dentist; Cone - Beam Computed Tomography
{"title":"The Assessment of Dentists’ Knowledge Regarding Indications of Cone Beam Computed Tomography in Qazvin, Iran","authors":"M. Tofangchiha, F. Arianfar, M. Bakhshi, M. Khorasani","doi":"10.17795/BHS-25815","DOIUrl":"https://doi.org/10.17795/BHS-25815","url":null,"abstract":"Background: Cone beam computed tomography (CT) has recently become effective for oral and maxillofacial imaging. \u0000Objectives: The aim of this study was to assess the knowledge of dentists regarding cone beam computed tomography. \u0000Materials and Methods: In this descriptive cross sectional study, a questionnaire regarding cone-beam computed tomography (CBCT) was distributed amongst 100 dentists (general and specialist) in Qazvin, Iran. Their level of knowledge was compared in each section on the basis of age, gender, years of employment and last educational status and analyzed by the SPSS software and Mann-Whitney test. \u0000Results: Data analysis showed that 4% of dentists had very low, 16% had low, 50% had medium, 19% had good and 11% had in very good level of knowledge. The average of dentists’ knowledge was 57 ± 18. According to the statistical results, there was a significant difference between level of knowledge and age, years of employment and educational degree (P 0.05). \u0000Conclusions: Overall, dentists had an average level of knowledge for CBCT. It is recommended for qualification programs to be held for dentists to strengthen their awareness toward cone beam computed tomography. \u0000Keywords:Knowledge; Dentist; Cone - Beam Computed Tomography","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89925960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Aslanimehr, M. S. Ghazvini, Saman Saadat, A. Barikani, T. Farivar
Results: Out of the 240 female participants, 184 (76.7%) were symptomatic and 56 (23.3%) were asymptomatic cases. The mean age of cases was 37.1 ± 0.9 years. Twenty (8.3%) of the 240 samples were diagnosed as Chlamydia positive according to PCR results. The prevalence of asymptomatic C. trachomatis infections was six (10.7%), while there were 14 (7.6%) in symptomatic cases. Although positive PCR results have shown in women with vaginosis (7.1%), abortion (5.1%), premature birth and low birth weight below 2.5 kg (7.7%) but the chi-square test did not indicate a significant relationship between positive PCR test results and these symptoms. Conclusions: The results of this study showed that there was a high prevalence of C. trachomatis infection among both symptomatic and asymptomatic women. Therefore, a screening test for C. trachomatis infection is recommended for all women who refer to the genitourinary medicine clinic. Screening programs are important for cost effectiveness calculations of C. trachomatis infections especially in asymptomatic cases.
{"title":"Frequency of Chlamydia trachomatis in Endocervical Samples of Women Referred to a Gynecology Hospital in Qazvin, Iran","authors":"M. Aslanimehr, M. S. Ghazvini, Saman Saadat, A. Barikani, T. Farivar","doi":"10.17795/BHS-27009","DOIUrl":"https://doi.org/10.17795/BHS-27009","url":null,"abstract":"Results: Out of the 240 female participants, 184 (76.7%) were symptomatic and 56 (23.3%) were asymptomatic cases. The mean age of cases was 37.1 ± 0.9 years. Twenty (8.3%) of the 240 samples were diagnosed as Chlamydia positive according to PCR results. The prevalence of asymptomatic C. trachomatis infections was six (10.7%), while there were 14 (7.6%) in symptomatic cases. Although positive PCR results have shown in women with vaginosis (7.1%), abortion (5.1%), premature birth and low birth weight below 2.5 kg (7.7%) but the chi-square test did not indicate a significant relationship between positive PCR test results and these symptoms. Conclusions: The results of this study showed that there was a high prevalence of C. trachomatis infection among both symptomatic and asymptomatic women. Therefore, a screening test for C. trachomatis infection is recommended for all women who refer to the genitourinary medicine clinic. Screening programs are important for cost effectiveness calculations of C. trachomatis infections especially in asymptomatic cases.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89721782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Conclusions: Maternal physical activity affects children’s physical activity, and is affected by mother’s self-efficacy. Yet based on the findings of this study, regarding the role model effects of mothers in children aged 10-12 years, researchers proposed that interventions related to physical activity in children would work better if they are set to increase self-efficacy in mothers, which in turn lead to increased physical activity in children.
{"title":"The Effects of Mothers' Self-Efficacy on Children's Physical Activity","authors":"K. Hosseinzadeh, S. Niknami, A. Hidarnia","doi":"10.17795/BHS-25731","DOIUrl":"https://doi.org/10.17795/BHS-25731","url":null,"abstract":"Conclusions: Maternal physical activity affects children’s physical activity, and is affected by mother’s self-efficacy. Yet based on the findings of this study, regarding the role model effects of mothers in children aged 10-12 years, researchers proposed that interventions related to physical activity in children would work better if they are set to increase self-efficacy in mothers, which in turn lead to increased physical activity in children.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87100926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Rostami, N. S. Bashir, Parviz Pirniakan, N. Masoudi
Objectives: In this paper, we report the expression and assembly of VLP in cells of E. coli expressing the CMVCP gene. Materials and Methods: In this study, the CMV-CP gene was released from a previously prepared cloning vector. Then, the CMV-CP was ligated into the expression vector. Sequencing was done by Marcrogen, Inc. (South Korea). A recombinant plasmid was transferred to E.coli isolate Rosetta. After inducing by isopropyl thiogalactosides, the molecular weight of the expressed protein was determined by SDS-PAGE. The extraction of proteins was done by NATURE method to see the possible presence of CMV-like particles. Results: CMV-CP was detected by Western blotting by a CMV specific polyclonal antibody and conjugate. The protein extracted from the CP producing clone was studied under a JEOL 100-CXII transmission electron microscope with 100000× magnification at an acceleration voltage of 100 kV. Conclusions: The results showed that the CP gene was expressed in the prokaryotic system successfully and was assembled into the CMVlike particle.
{"title":"Expression of Cucumber Mosaic Virus Coat Protein and Its Assembly into Virus Like Particles","authors":"A. Rostami, N. S. Bashir, Parviz Pirniakan, N. Masoudi","doi":"10.17795/BHS-24729","DOIUrl":"https://doi.org/10.17795/BHS-24729","url":null,"abstract":"Objectives: In this paper, we report the expression and assembly of VLP in cells of E. coli expressing the CMVCP gene. Materials and Methods: In this study, the CMV-CP gene was released from a previously prepared cloning vector. Then, the CMV-CP was ligated into the expression vector. Sequencing was done by Marcrogen, Inc. (South Korea). A recombinant plasmid was transferred to E.coli isolate Rosetta. After inducing by isopropyl thiogalactosides, the molecular weight of the expressed protein was determined by SDS-PAGE. The extraction of proteins was done by NATURE method to see the possible presence of CMV-like particles. Results: CMV-CP was detected by Western blotting by a CMV specific polyclonal antibody and conjugate. The protein extracted from the CP producing clone was studied under a JEOL 100-CXII transmission electron microscope with 100000× magnification at an acceleration voltage of 100 kV. Conclusions: The results showed that the CP gene was expressed in the prokaryotic system successfully and was assembled into the CMVlike particle.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81958139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Valizadeh, Tayebeh Fakheri, R. Mahmoudi, F. Katiraee, P. Gajarbeygi
Background: Satureja is a genus belonging to the aromatic plants of Lamiaceae family. The genus Satureja L. (Lamiaceae) comprises more than 30 species of aromatic herbs and shrubs, widely distributed over the Mediterranean region. The genus is represented by 14 species in Iran of which, eight are endemic. Many species of the genus Satureja are reported to have aromatic and medicinal properties. The leaves, flowers, and stems of this plant are used as herbal tea and in treatment of various ailments in traditional medicine. Objectives: The aim of this study was to investigate antimicrobial and antioxidant activity of essential oil (EO) of Satureja hortensis (Lamiaceae) that grows in Sabalan Mountain (Ardebil province, Iran). Materials and Methods: This EO was tested in vitro against two bacterial species by disk and agar well diffusion methods and against four bacterial species and three Candida strains by broth microdilution method. Total phenol, flavonoid, and free radical scavenging activity of EO were evaluated. Results: Total phenolic content was determined by using Folin-Ciocalteu reagent (32.65 mg/g), which was presented as gallic acid equivalent in 1 g of sample. IC50 of EO and ascorbic acid in DPPH method were respectively 277.9 and 19.34 µg. Minimum inhibitory concentration of the EO of S. hortensis against Salmonella typhimurium, Listeria monocytogenes, Escherichia coli, and Bacillus cereus was respectively 2.5%, 2.5%, 5%, and 2.5%. The inhibition zone of EO in disk and agar well diffusion method showed that inhibitory zone on B. cereus was higher than that on S. typhimurium in both methods and B. cereus was more sensitive to EO. MIC to minimal fungicidal concentration (MFC) ratio of S. hortensis EO against Candida albicans, Candida parapsilosis, and Candida krusei in broth microdilution method were respectively 0.048%:0.048%, 0.024%:0.024%, and 0.012%:0.012%. Conclusions: The data of the study clearly indicated that the EO of S. hortensis has a strong antioxidant, antibacterial, and antifungal activity.
{"title":"Evaluation of Antioxidant, Antibacterial, and Antifungal Properties of Satureja hortensis Essential Oil","authors":"S. Valizadeh, Tayebeh Fakheri, R. Mahmoudi, F. Katiraee, P. Gajarbeygi","doi":"10.17795/BHS-24733","DOIUrl":"https://doi.org/10.17795/BHS-24733","url":null,"abstract":"Background: Satureja is a genus belonging to the aromatic plants of Lamiaceae family. The genus Satureja L. (Lamiaceae) comprises more than 30 species of aromatic herbs and shrubs, widely distributed over the Mediterranean region. The genus is represented by 14 species in Iran of which, eight are endemic. Many species of the genus Satureja are reported to have aromatic and medicinal properties. The leaves, flowers, and stems of this plant are used as herbal tea and in treatment of various ailments in traditional medicine. Objectives: The aim of this study was to investigate antimicrobial and antioxidant activity of essential oil (EO) of Satureja hortensis (Lamiaceae) that grows in Sabalan Mountain (Ardebil province, Iran). Materials and Methods: This EO was tested in vitro against two bacterial species by disk and agar well diffusion methods and against four bacterial species and three Candida strains by broth microdilution method. Total phenol, flavonoid, and free radical scavenging activity of EO were evaluated. Results: Total phenolic content was determined by using Folin-Ciocalteu reagent (32.65 mg/g), which was presented as gallic acid equivalent in 1 g of sample. IC50 of EO and ascorbic acid in DPPH method were respectively 277.9 and 19.34 µg. Minimum inhibitory concentration of the EO of S. hortensis against Salmonella typhimurium, Listeria monocytogenes, Escherichia coli, and Bacillus cereus was respectively 2.5%, 2.5%, 5%, and 2.5%. The inhibition zone of EO in disk and agar well diffusion method showed that inhibitory zone on B. cereus was higher than that on S. typhimurium in both methods and B. cereus was more sensitive to EO. MIC to minimal fungicidal concentration (MFC) ratio of S. hortensis EO against Candida albicans, Candida parapsilosis, and Candida krusei in broth microdilution method were respectively 0.048%:0.048%, 0.024%:0.024%, and 0.012%:0.012%. Conclusions: The data of the study clearly indicated that the EO of S. hortensis has a strong antioxidant, antibacterial, and antifungal activity.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85442885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
and statistical tests including t-test and chi-square tests. Results: Of the 484 patients, 294 (60.7%) were male and 190 (39.3%) were female. Most cases of carcinoma were found in basal cells (77.7%) and the rest of squamous cells. The most common sites of involvement were the head and scalp. The most frequent predisposing factors were working in the open air (70.7%), history of previous radiation for treatment of tenia capitis (26.1%), and chronic skin diseases such as burn eschar, chronic lesion, fistula and actinic keratosis (35.7%). The most prevalent Fitzpatrick skin phenotypes were II or III (75.4%). There were significant correlations between the incidence of NMSC and hookah smoking and oral contraceptive pill (OCP) consumption. Conclusions: Skin phenotypes II and III were the most common types found in the present study and this could be due to the higher frequency of these phenotypes among the study population. The lower incidence of NMSC in areas other than the neck and scalp could be associated with the importance of covering style used by the population under study. Known risk factors for the incidence of NMSC were also observed in the present study. A history of radiotherapy for treatment of tenia capitis was observed in a considerable percentage of patients, which could lead to the incidence of cancer several decades later. Further studies are needed to determine the role of hookah smoking and the use of OCP in the occurrence of NMSC.
{"title":"Epidemiological Study of Non-Melanoma Skin Cancers Qazvin Province, Iran","authors":"Akram Beheshtiroy, F. Hajmanoochehri","doi":"10.17795/BHS-25362","DOIUrl":"https://doi.org/10.17795/BHS-25362","url":null,"abstract":"and statistical tests including t-test and chi-square tests. Results: Of the 484 patients, 294 (60.7%) were male and 190 (39.3%) were female. Most cases of carcinoma were found in basal cells (77.7%) and the rest of squamous cells. The most common sites of involvement were the head and scalp. The most frequent predisposing factors were working in the open air (70.7%), history of previous radiation for treatment of tenia capitis (26.1%), and chronic skin diseases such as burn eschar, chronic lesion, fistula and actinic keratosis (35.7%). The most prevalent Fitzpatrick skin phenotypes were II or III (75.4%). There were significant correlations between the incidence of NMSC and hookah smoking and oral contraceptive pill (OCP) consumption. Conclusions: Skin phenotypes II and III were the most common types found in the present study and this could be due to the higher frequency of these phenotypes among the study population. The lower incidence of NMSC in areas other than the neck and scalp could be associated with the importance of covering style used by the population under study. Known risk factors for the incidence of NMSC were also observed in the present study. A history of radiotherapy for treatment of tenia capitis was observed in a considerable percentage of patients, which could lead to the incidence of cancer several decades later. Further studies are needed to determine the role of hookah smoking and the use of OCP in the occurrence of NMSC.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81686598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: S100A8 and S100A9 as two subunits of heterodimeric calprotectin are identified mainly in leukocytes and are involved in inflammatory processes and several cancerous pathogens. This study was performed in order to evaluate the interaction of recombinant calprotectin subunits and to estimate calprotectin’s tertiary and secondary structures. Objectives: The aim of this study was to investigate the effects of calcium in calprotectin dimerization as a cancer biomarker. Materials and Methods: Heterodimeric calprotectin was formed with incubation of recombinant S100A8 and S100A9 subunits in the presence of Ca (1 mM), at 25 C for 15 minutes. Tertiary and secondary structures of S100A8, S100A9 and their complex were investigated, using fluorescence and circular dichroism (CD) spectroscopy, respectively. Results: Interaction of S100A8 and S100A9 in the presence of Ca2+ were revealed by decreasing the emission intensity of intrinsic fluorescence and increasing of the external fluorescence and also changes in the CD spectra of subunits after Ca2+ interactions. Conclusions: The expression of recombinant calprotectin, as an effective protein, can help in diagnosis or treatment of inflammatory and cancer processes in the future. Furthermore, Ca2+ induced a partial change in secondary and tertiary structure of calprotectin subunits and this change is probably necessary for protein dimerization.
{"title":"The Role of Calcium in Calprotectin Dimerization as a Cancer Biomarker","authors":"F. N. Nikoo, K. G. Chegini, R. N. Pour, N. Gheibi","doi":"10.17795/BHS-26379","DOIUrl":"https://doi.org/10.17795/BHS-26379","url":null,"abstract":"Background: S100A8 and S100A9 as two subunits of heterodimeric calprotectin are identified mainly in leukocytes and are involved in inflammatory processes and several cancerous pathogens. This study was performed in order to evaluate the interaction of recombinant calprotectin subunits and to estimate calprotectin’s tertiary and secondary structures. Objectives: The aim of this study was to investigate the effects of calcium in calprotectin dimerization as a cancer biomarker. Materials and Methods: Heterodimeric calprotectin was formed with incubation of recombinant S100A8 and S100A9 subunits in the presence of Ca (1 mM), at 25 C for 15 minutes. Tertiary and secondary structures of S100A8, S100A9 and their complex were investigated, using fluorescence and circular dichroism (CD) spectroscopy, respectively. Results: Interaction of S100A8 and S100A9 in the presence of Ca2+ were revealed by decreasing the emission intensity of intrinsic fluorescence and increasing of the external fluorescence and also changes in the CD spectra of subunits after Ca2+ interactions. Conclusions: The expression of recombinant calprotectin, as an effective protein, can help in diagnosis or treatment of inflammatory and cancer processes in the future. Furthermore, Ca2+ induced a partial change in secondary and tertiary structure of calprotectin subunits and this change is probably necessary for protein dimerization.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82647252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Gastric cancer is the second leading cause of cancer-related deaths worldwide and it seems that environmental and lifestyle factors and infection with Helicobacter pylori (H. pylori) have a major role in the etiology of gastric cancer. Objectives: The aim of this study was to investigate the presence of H. pylori DNA in archival gastric tissues of patients with gastric cancer disease by rapid, sensitive and specific technique of Scorpion real-time PCR. Patients and Methods: This retrospective cross-sectional study was performed during the year 2009, on 285 paraffin embedded gastric specimens of patients, who were pathologically proved to have gastric cancer and were admitted to Bou-Ali, Shahid Rajaie and Dehkhoda Hospitals and Bahar and Farzam Private Laboratory of Qazvin city, Iran. Results: The results of the Scorpion real-time PCR showed that H. pylori DNA was present in 8.42% of the total specimens. Modified McMullen’s staining of paraffin embedded sections were positive in ten patients. There was no significant relationship between the presence of H. pylori, sex, age and place of residence. Conclusions: Although the existence of H. pylori in gastric tissue samples of patients with gastric cancer is controversial however, our results showed that in our studied specimens a significant number of patients with gastric cancer had H. pylori colonization.
{"title":"Prevalence of Helicobacter pylori in Formaldehyde Fixed Paraffin Embedded Gastric Tissues of Gastric Cancer Patients by Scorpion Real-Time PCR Assay","authors":"A. Farzam, R. Najafipour, P. Johari, T. Farivar","doi":"10.17795/BHS-25836","DOIUrl":"https://doi.org/10.17795/BHS-25836","url":null,"abstract":"Background: Gastric cancer is the second leading cause of cancer-related deaths worldwide and it seems that environmental and lifestyle factors and infection with Helicobacter pylori (H. pylori) have a major role in the etiology of gastric cancer. Objectives: The aim of this study was to investigate the presence of H. pylori DNA in archival gastric tissues of patients with gastric cancer disease by rapid, sensitive and specific technique of Scorpion real-time PCR. Patients and Methods: This retrospective cross-sectional study was performed during the year 2009, on 285 paraffin embedded gastric specimens of patients, who were pathologically proved to have gastric cancer and were admitted to Bou-Ali, Shahid Rajaie and Dehkhoda Hospitals and Bahar and Farzam Private Laboratory of Qazvin city, Iran. Results: The results of the Scorpion real-time PCR showed that H. pylori DNA was present in 8.42% of the total specimens. Modified McMullen’s staining of paraffin embedded sections were positive in ten patients. There was no significant relationship between the presence of H. pylori, sex, age and place of residence. Conclusions: Although the existence of H. pylori in gastric tissue samples of patients with gastric cancer is controversial however, our results showed that in our studied specimens a significant number of patients with gastric cancer had H. pylori colonization.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77376525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
denaturation of the tyrosinase with and without the presence of palladium complex. The tertiary and secondary structures of tyrosinase were detected by fluorescent and Circular Dichroism (CD) techniques. Results: The inhibition modes of palladium complex were competitive in both activities of the enzyme with Ki values of 3.74 and 10.55 μM for cresolase and catecholase activities, respectively. In thermal denaturation, the melting points (T m ) of the enzyme were 59.4˚C and 51˚C for the sole enzyme and its treatment by palladium, respectively. In chemical denaturation, the magnitudes of half denaturant concentration (C m ) were 1 μM vs. 1.36μM and the free energy of Gibss (ΔG H2O ) were calculated 9.3 vs. 7.5 kJ/M for the sole enzyme and its treatment by palladium, respectively. Conclusions: In overall the palladium complex acted as a good inhibitor of tyrosinase and induced the enzyme thermodynamic and conformational instability, therefore it can be considered in the hyper expression of tyrosinase in melanoma cancer.
{"title":"Inhibitory Effects of a Palladium Complex on the Activity, Stability, and Structure of Tyrosinase Enzyme","authors":"N. Gheibi, Nasibe Yaghouby Nejad, M. Sahmani","doi":"10.17795/BHS-26373","DOIUrl":"https://doi.org/10.17795/BHS-26373","url":null,"abstract":"denaturation of the tyrosinase with and without the presence of palladium complex. The tertiary and secondary structures of tyrosinase were detected by fluorescent and Circular Dichroism (CD) techniques. Results: The inhibition modes of palladium complex were competitive in both activities of the enzyme with Ki values of 3.74 and 10.55 μM for cresolase and catecholase activities, respectively. In thermal denaturation, the melting points (T m ) of the enzyme were 59.4˚C and 51˚C for the sole enzyme and its treatment by palladium, respectively. In chemical denaturation, the magnitudes of half denaturant concentration (C m ) were 1 μM vs. 1.36μM and the free energy of Gibss (ΔG H2O ) were calculated 9.3 vs. 7.5 kJ/M for the sole enzyme and its treatment by palladium, respectively. Conclusions: In overall the palladium complex acted as a good inhibitor of tyrosinase and induced the enzyme thermodynamic and conformational instability, therefore it can be considered in the hyper expression of tyrosinase in melanoma cancer.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87107788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Farivar, Babak Mamnoon, M. K. Arzenani, D. Ilghari
Background: Contamination of therapeutic recombinant proteins with residual host cell DNA must be controlled under the regulatory standards. Objectives: The current study established a new rapid, sensitive real time polymerase chain reaction (PCR) approach to measure the reliably of the residual Escherichia coli (E. coli) host cell genomic DNA in the recombinant streptokinase and alfa interferon preparations. Materials and Methods: In this assay, a specific primer pair was utilized to amplify a 115 base pair sequence inside the E. coli 16S rRNA using SYBR Green Chemistry. This method enabled the authors to detect a very small quantity of the residual genomic DNA, as low as 0.8 pg, in the protein-based drugs. This method can, therefore, offer a dependable way to quantitatively analyze the major contaminant of biopharmaceutical products, the host cell DNA, during the manufacturing process. Results: SYBR Green PCR master mix may contain a source of DNA contamination during its manufacturing process. Conclusions: The current study data showed that E. coli host cell DNA contamination in streptokinase and alfa interferon manufactured in the Pasteur institute of Iran is much lower than the safety limits suggested by the FDA.
背景:治疗性重组蛋白与宿主细胞DNA残留的污染必须控制在监管标准之下。目的:建立一种新的快速、灵敏的实时聚合酶链反应(PCR)方法，可靠地测定重组链激酶和α干扰素制剂中残留的大肠杆菌(E. coli)宿主细胞基因组DNA。材料和方法:在本实验中，使用SYBR Green Chemistry，利用特定的引物对扩增大肠杆菌16S rRNA中的115碱基对序列。这种方法使作者能够在蛋白质药物中检测到非常少量的残留基因组DNA，低至0.8 pg。因此，该方法可以提供一种可靠的方法来定量分析生物制药产品在生产过程中的主要污染物——宿主细胞DNA。结果:SYBR Green PCR母品在生产过程中可能含有DNA污染源。结论:目前的研究数据显示，伊朗巴斯德研究所生产的链激酶和α干扰素中大肠杆菌宿主细胞DNA污染远低于FDA建议的安全限值。
{"title":"Novel Real Time Polymerase Chain Reaction Approach for Rapid Detection of the Residual Escherichia coli Genomic DNA in Biopharmaceutical Products Establishment of Real Time Polymerase Chain Reaction to Detect Residual gDNA","authors":"T. Farivar, Babak Mamnoon, M. K. Arzenani, D. Ilghari","doi":"10.17795/BHS-26375","DOIUrl":"https://doi.org/10.17795/BHS-26375","url":null,"abstract":"Background: Contamination of therapeutic recombinant proteins with residual host cell DNA must be controlled under the regulatory standards. Objectives: The current study established a new rapid, sensitive real time polymerase chain reaction (PCR) approach to measure the reliably of the residual Escherichia coli (E. coli) host cell genomic DNA in the recombinant streptokinase and alfa interferon preparations. Materials and Methods: In this assay, a specific primer pair was utilized to amplify a 115 base pair sequence inside the E. coli 16S rRNA using SYBR Green Chemistry. This method enabled the authors to detect a very small quantity of the residual genomic DNA, as low as 0.8 pg, in the protein-based drugs. This method can, therefore, offer a dependable way to quantitatively analyze the major contaminant of biopharmaceutical products, the host cell DNA, during the manufacturing process. Results: SYBR Green PCR master mix may contain a source of DNA contamination during its manufacturing process. Conclusions: The current study data showed that E. coli host cell DNA contamination in streptokinase and alfa interferon manufactured in the Pasteur institute of Iran is much lower than the safety limits suggested by the FDA.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80263557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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