A. Nikpey, M. Ghalenoei, Ali Safary Variani, H. Nadri
Background: Noise at Intensive Care Units (ICU) has an adverse effect on patients and ICU staff. There are some evidences that sleep, recovery from critical illness and average background noise in hospitals as recommended by the US Environmental Protection Agency (EPA) and World Health Organization (WHO) should not exceed 30 A-weighted decibel (dBA) and peaks during night time should be less than 40 dBA. This survey was conducted to measure noise levels and their relationship with the time of the day and location in the ICU. Objectives: The objectives of this study were to measure noise levels and evaluate their relationship with time of day and location in the ICU. Materials and Methods: This cross sectional study was conducted in a public university hospital, namely Qazvin University of Medical Sciences, Qazvin, Iran. Noise levels were measured with SLM Sound level meter (model: Tes-1443) during 24 hours with the equivalent sound level (LEQ), maximum (Max) and peak sound pressure based on the ISO 9612.this tool can measure in the range of 30 to 110 dB dynamic network. While frequency A, fast time scale networks with 125 ms fast response microphones were selected. This method says that measuring point must have distance 1.5 meter from the wall at a height of 1.25 m above ground level. At the bedside of patients measurement done by 3 TES model 1353 H Tool by a Taiwanese company. Results: This survey showed that the Equivalent Sound Level (Leq) in ICU was much higher than the standard level. The Maximum Sound Level (Lmax) in most places was 84 - 89 dBA and just in one measurement in the Internal ICU reached 90 dB. The average level of Leq in ICU was 70 dB. Conclusions: Equivalent noise level and Noise Criteria in ward remarkably exceeds the standards levels. This condition will be produce Dangerous circumstances for admitted patients in ward.
{"title":"Evaluating the Noise level at Qazvin University Hospital's Intensive Care Units","authors":"A. Nikpey, M. Ghalenoei, Ali Safary Variani, H. Nadri","doi":"10.17795/BHS-28456","DOIUrl":"https://doi.org/10.17795/BHS-28456","url":null,"abstract":"Background: Noise at Intensive Care Units (ICU) has an adverse effect on patients and ICU staff. There are some evidences that sleep, recovery from critical illness and average background noise in hospitals as recommended by the US Environmental Protection Agency (EPA) and World Health Organization (WHO) should not exceed 30 A-weighted decibel (dBA) and peaks during night time should be less than 40 dBA. This survey was conducted to measure noise levels and their relationship with the time of the day and location in the ICU. Objectives: The objectives of this study were to measure noise levels and evaluate their relationship with time of day and location in the ICU. Materials and Methods: This cross sectional study was conducted in a public university hospital, namely Qazvin University of Medical Sciences, Qazvin, Iran. Noise levels were measured with SLM Sound level meter (model: Tes-1443) during 24 hours with the equivalent sound level (LEQ), maximum (Max) and peak sound pressure based on the ISO 9612.this tool can measure in the range of 30 to 110 dB dynamic network. While frequency A, fast time scale networks with 125 ms fast response microphones were selected. This method says that measuring point must have distance 1.5 meter from the wall at a height of 1.25 m above ground level. At the bedside of patients measurement done by 3 TES model 1353 H Tool by a Taiwanese company. Results: This survey showed that the Equivalent Sound Level (Leq) in ICU was much higher than the standard level. The Maximum Sound Level (Lmax) in most places was 84 - 89 dBA and just in one measurement in the Internal ICU reached 90 dB. The average level of Leq in ICU was 70 dB. Conclusions: Equivalent noise level and Noise Criteria in ward remarkably exceeds the standards levels. This condition will be produce Dangerous circumstances for admitted patients in ward.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76467664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Glioblastoma is a type of brain tumor with poor response to available therapies, and shows high rate of mortality. Despite remarkable advancements in our knowledge about cytogenetic and pathophysiologic features of glioblastoma, current treatment strategies are mainly based on cytotoxic drugs; however, these therapeutic approaches are facing progressive failure because of the resistant nature of glioblastomas. In the recent years, however, promising results have emerged owing to targeted therapies toward molecular pathways within cancerous cells. Unfolded Protein Response (UPR) is a remarkable signaling pathway that triggers both apoptosis and survival pathways within cells, and therefore induces UPR-related apoptotic pathways in cancer cells by ER stress inducers. Objectives: Recently, the role of Bee venom (Bv), which contains powerful bioactive peptides, in inducing UPR-related apoptosis was revealed in cancer cell lines. Nevertheless, currently there are no reports of Bv potential ability in induction of UPR apoptotic routes in glioblastoma. The aim of current study was to evaluate possible role of Bee venome in inducing of UPR pathway within A172 glioblastoma cell line. Materials and Methods: We treated the A172 glioblastoma cell line with different Bv doses, and assessed UPR-related genes expression by real-time Polymerase Chain Reaction (PCR). Results: The IC50 of Bv for the studied cell line was 28 μg/mL. Furthermore, we observed that Bv can induce UPR target genes (Grp94 and Gadd153) over-expression through a dose-dependent mechanism. Conclusions: Our results suggest the potential role of Bv as a therapeutic agent for glioblastomas. Keywords: Glioblastoma; A172 Cell Line; Unfolded Protein Response; Bee Venom
背景:胶质母细胞瘤是一种对现有治疗反应较差且死亡率高的脑肿瘤。尽管我们对胶质母细胞瘤的细胞遗传学和病理生理特征的了解取得了显著进展，但目前的治疗策略主要基于细胞毒性药物;然而，由于胶质母细胞瘤的耐药性，这些治疗方法正面临着逐渐失败。然而，近年来，由于针对癌细胞内部分子途径的靶向治疗，出现了令人鼓舞的结果。未折叠蛋白反应(Unfolded Protein Response, UPR)是一种重要的信号通路，可触发细胞内的凋亡和存活通路，因此可通过内质网应激诱导剂诱导癌细胞中UPR相关的凋亡通路。目的:蜂毒(Bv)含有强大的生物活性肽，近年来发现其在肿瘤细胞中诱导凋亡的作用。然而，目前还没有关于Bv在胶质母细胞瘤中诱导UPR凋亡途径的潜在能力的报道。本研究的目的是评估蜜蜂毒液在A172胶质母细胞瘤细胞系中诱导UPR通路的可能作用。材料与方法:用不同剂量的Bv处理A172胶质母细胞瘤细胞系，采用实时聚合酶链反应(PCR)检测uprr相关基因的表达。结果:Bv的IC50为28 μg/mL。此外，我们观察到Bv通过剂量依赖机制诱导UPR靶基因(Grp94和Gadd153)过表达。结论:我们的研究结果提示Bv作为胶质母细胞瘤治疗剂的潜在作用。关键词:胶质母细胞瘤;A172细胞系;未折叠蛋白反应;蜂毒
{"title":"Bee Venom Induces Unfolded Protein Response in A172 Glioblastoma Cell Line","authors":"A. Bazi, M. Gholamin, M. Sisakht, M. Keramati","doi":"10.17795/BHS-27547","DOIUrl":"https://doi.org/10.17795/BHS-27547","url":null,"abstract":"Background: Glioblastoma is a type of brain tumor with poor response to available therapies, and shows high rate of mortality. Despite remarkable advancements in our knowledge about cytogenetic and pathophysiologic features of glioblastoma, current treatment strategies are mainly based on cytotoxic drugs; however, these therapeutic approaches are facing progressive failure because of the resistant nature of glioblastomas. In the recent years, however, promising results have emerged owing to targeted therapies toward molecular pathways within cancerous cells. Unfolded Protein Response (UPR) is a remarkable signaling pathway that triggers both apoptosis and survival pathways within cells, and therefore induces UPR-related apoptotic pathways in cancer cells by ER stress inducers. \u0000Objectives: Recently, the role of Bee venom (Bv), which contains powerful bioactive peptides, in inducing UPR-related apoptosis was revealed in cancer cell lines. Nevertheless, currently there are no reports of Bv potential ability in induction of UPR apoptotic routes in glioblastoma. The aim of current study was to evaluate possible role of Bee venome in inducing of UPR pathway within A172 glioblastoma cell line. \u0000Materials and Methods: We treated the A172 glioblastoma cell line with different Bv doses, and assessed UPR-related genes expression by real-time Polymerase Chain Reaction (PCR). \u0000Results: The IC50 of Bv for the studied cell line was 28 μg/mL. Furthermore, we observed that Bv can induce UPR target genes (Grp94 and Gadd153) over-expression through a dose-dependent mechanism. \u0000Conclusions: Our results suggest the potential role of Bv as a therapeutic agent for glioblastomas. \u0000Keywords: Glioblastoma; A172 Cell Line; Unfolded Protein Response; Bee Venom","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"262 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74144596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Bijani, R. Q. Barqi, A. Pahlevan, M. Sarokhani, Shiva Leghaie, Ebrahim Amini
Background: In 2009, a pandemic associated with a new type of influenza A virus (H1N1) affected many countries worldwide. After five years of silence, in 2015 we encountered another outbreak of H1N1 influenza A. Objectives: The present study aimed to study the epidemiological and clinical features of this disease in the cold and dry climate of Qazvin province, Iran in the last epidemic, during 2009. Patients and Methods: This was a cross-sectional study in which the demographic characteristics and clinical manifestations of confirmed cases of influenza A virus (H1N1) in the province of Qazvin were investigated. The definite diagnosis of cases was performed using real time Polymerase Chain Reaction (PCR) on oropharyngeal washing specimens from adults and throat swabs from children and severely ill patients. Results: During the time course between July to December 2009, 76 confirmed cases of influenza A (H1N1) were discovered in the province of Qazvin. The mean age of patients was 25.67 ± 16.9 years. The most affected people were students and housewives. Coughing was found to be the most common clinical symptom (96.1%) followed by fever (92.1%), myalgia (48.5%), and diarrhea and vomiting (34.2%). In laboratory confirmed patients, 62 were hospitalized and two cases deceased. Regarding the total population of the Qazvin province (1,100,000), the rate of hospitalization was calculated at 5.42 per 100,000 individuals, with a mortality rate of 0.175 per 100,000 individuals (3.2% of hospitalized cases). Conclusions: Concerning the higher prevalence of disease in younger age groups, and more severe disease in high-risk groups, including overweight patients and pregnant women, the authors recommend special attention to clinical symptoms such as diarrhea and vomiting, cough, myalgia and fever in patients with cold symptoms. Also, for severely ill patients, the allocation of adequate intensive care units should be of prime importance.
{"title":"Study of the Epidemiological Features and Clinical Manifestations of the Preceding Epidemic of Influenza A (H1N1) as a Guide for Dealing With the 2015 Outbreak in the Qazvin Province, Iran","authors":"B. Bijani, R. Q. Barqi, A. Pahlevan, M. Sarokhani, Shiva Leghaie, Ebrahim Amini","doi":"10.17795/BHS-28414","DOIUrl":"https://doi.org/10.17795/BHS-28414","url":null,"abstract":"Background: In 2009, a pandemic associated with a new type of influenza A virus (H1N1) affected many countries worldwide. After five years of silence, in 2015 we encountered another outbreak of H1N1 influenza A. Objectives: The present study aimed to study the epidemiological and clinical features of this disease in the cold and dry climate of Qazvin province, Iran in the last epidemic, during 2009. Patients and Methods: This was a cross-sectional study in which the demographic characteristics and clinical manifestations of confirmed cases of influenza A virus (H1N1) in the province of Qazvin were investigated. The definite diagnosis of cases was performed using real time Polymerase Chain Reaction (PCR) on oropharyngeal washing specimens from adults and throat swabs from children and severely ill patients. Results: During the time course between July to December 2009, 76 confirmed cases of influenza A (H1N1) were discovered in the province of Qazvin. The mean age of patients was 25.67 ± 16.9 years. The most affected people were students and housewives. Coughing was found to be the most common clinical symptom (96.1%) followed by fever (92.1%), myalgia (48.5%), and diarrhea and vomiting (34.2%). In laboratory confirmed patients, 62 were hospitalized and two cases deceased. Regarding the total population of the Qazvin province (1,100,000), the rate of hospitalization was calculated at 5.42 per 100,000 individuals, with a mortality rate of 0.175 per 100,000 individuals (3.2% of hospitalized cases). Conclusions: Concerning the higher prevalence of disease in younger age groups, and more severe disease in high-risk groups, including overweight patients and pregnant women, the authors recommend special attention to clinical symptoms such as diarrhea and vomiting, cough, myalgia and fever in patients with cold symptoms. Also, for severely ill patients, the allocation of adequate intensive care units should be of prime importance.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81429829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Rostami, N. S. Bashir, D. Koolivand, M. Hajizadeh
Background: Cucumber mosaic virus (CMV) has isometric particles with a diameter of about 28 - 29 nm. Detection and prevention are the critical steps in the control of plant viruses. Detection in a large number of samples is still done by serological methods due to their robustness and perhaps low cost. Objectives: To this end, our aim was to express the CMV CP gene in E. coli to be used as the antigen for antibody production in the future. Materials and Methods: Coat Protein (CP) gene cDNA from an isolate (B13) of Cucumber Mosaic Virus (CMV) was subcloned from pTZ57RCMVCP to pET21a expression vector and transformed to E. coli strain Rosetta. Expression of CMV CP was successful and confirmed by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), wherein a ~30- kDa protein band was revealed. Induction by Isopropyl-Thiogalactoside (IPTG) at final concentrations of 0.5 to 2 mM appeared to produce similar results as to the amount of the expressed protein, which was judged by intensity of the band on SDS-PAGE. Results: The identity of the expressed protein was confirmed by immunoassays such as western blot, Dot-Immunobinding Assay (DIBA) and Enzyme-Linked Immunosorbent Assay (ELISA) by the use of anti-CMV antibody. Conclusions: This is the first report of expression of CMV CP gene in Iran, which is important for the preparation of anti-CMV antibody and paving the way for the use of the virus coat protein as a nanomaterial.
{"title":"Serological Methods to Confirm Expression of Coat Protein Gene From an Iranian Isolate of Cucumber Mosaic Virus in Escherichia coli","authors":"A. Rostami, N. S. Bashir, D. Koolivand, M. Hajizadeh","doi":"10.17795/BHS-28253","DOIUrl":"https://doi.org/10.17795/BHS-28253","url":null,"abstract":"Background: Cucumber mosaic virus (CMV) has isometric particles with a diameter of about 28 - 29 nm. Detection and prevention are the critical steps in the control of plant viruses. Detection in a large number of samples is still done by serological methods due to their robustness and perhaps low cost. Objectives: To this end, our aim was to express the CMV CP gene in E. coli to be used as the antigen for antibody production in the future. Materials and Methods: Coat Protein (CP) gene cDNA from an isolate (B13) of Cucumber Mosaic Virus (CMV) was subcloned from pTZ57RCMVCP to pET21a expression vector and transformed to E. coli strain Rosetta. Expression of CMV CP was successful and confirmed by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), wherein a ~30- kDa protein band was revealed. Induction by Isopropyl-Thiogalactoside (IPTG) at final concentrations of 0.5 to 2 mM appeared to produce similar results as to the amount of the expressed protein, which was judged by intensity of the band on SDS-PAGE. Results: The identity of the expressed protein was confirmed by immunoassays such as western blot, Dot-Immunobinding Assay (DIBA) and Enzyme-Linked Immunosorbent Assay (ELISA) by the use of anti-CMV antibody. Conclusions: This is the first report of expression of CMV CP gene in Iran, which is important for the preparation of anti-CMV antibody and paving the way for the use of the virus coat protein as a nanomaterial.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78633217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Bijani, A. Pahlevan, R. Qasemi-Barqi, M. Sarokhani
Background: Emergence of a novel swine-origin influenza A (H1N1) pandemic since 2009 attracted the attention of scientists to characterize epidemiological features and clinical manifestations of this disease in comparison to seasonal flu in different parts of the world. Objectives: The goal of this investigation was to compare these features in confirmed cases of swine-origin influenza A (H1N1) and seasonal flu in the 2009 epidemic in Qazvin province, Iran. Patients and Methods: This cross sectional study was performed during 2009 in the Qazvin province. The epidemiological characteristics and clinical manifestations of all cases with severe flu-like manifestations were registered. Diagnosis of confirmed cases of both groups was performed by Real Time-Polymerase Chain Reaction (RT-PCR) on respiratory secretions of positive cases of swine-origin influenza A (H1N1) and seasonal influenza that were entered in the study. Analysis of quantitative data was performed using paired t-test and those of qualitative variables by chi square and Fisher’s exact test. Results: Among a total of 518 patients with clinical signs of severe influenza throughout the Qazvin province, 76 confirmed cases of swine-origin influenza A (H1N1) and 36 cases of other types of influenza A (seasonal influenza) were detected. The mean age of the first group was 25.67 ± 16.9 years and that of the second group was 36.03 ± 19.8, with a significant difference between the two groups (P < 0.01). The appearance of diarrhea was significantly higher in patients with swine-origin influenza compared to those with seasonal influenza (P < 0.005). There was no statistically significant difference in the number of hospitalizations, need for intensive care, assisted ventilation, and mortality rate between the two groups. Conclusions: Higher prevalence of disease in younger individuals, higher rate of gastrointestinal manifestations and occurrence outside of the epidemic season, were the most important characteristics of swine-origin influenza in comparison to seasonal influenza, in the 2009 pandemic of Qazvin province, Iran. Keywords: Influenza A Virus, H1N1 Subtype; Epidemiology
{"title":"Swine-Origin Influenza A (H1N1) and Seasonal Influenza in Qazvin Province, Iran: Comparison of Epidemiological Features, Clinical Manifestations and Outcome of the 2009 Pandemic","authors":"B. Bijani, A. Pahlevan, R. Qasemi-Barqi, M. Sarokhani","doi":"10.17795/BHS-26216","DOIUrl":"https://doi.org/10.17795/BHS-26216","url":null,"abstract":"Background: Emergence of a novel swine-origin influenza A (H1N1) pandemic since 2009 attracted the attention of scientists to characterize epidemiological features and clinical manifestations of this disease in comparison to seasonal flu in different parts of the world. \u0000Objectives: The goal of this investigation was to compare these features in confirmed cases of swine-origin influenza A (H1N1) and seasonal flu in the 2009 epidemic in Qazvin province, Iran. \u0000Patients and Methods: This cross sectional study was performed during 2009 in the Qazvin province. The epidemiological characteristics and clinical manifestations of all cases with severe flu-like manifestations were registered. Diagnosis of confirmed cases of both groups was performed by Real Time-Polymerase Chain Reaction (RT-PCR) on respiratory secretions of positive cases of swine-origin influenza A (H1N1) and seasonal influenza that were entered in the study. Analysis of quantitative data was performed using paired t-test and those of qualitative variables by chi square and Fisher’s exact test. \u0000Results: Among a total of 518 patients with clinical signs of severe influenza throughout the Qazvin province, 76 confirmed cases of swine-origin influenza A (H1N1) and 36 cases of other types of influenza A (seasonal influenza) were detected. The mean age of the first group was 25.67 ± 16.9 years and that of the second group was 36.03 ± 19.8, with a significant difference between the two groups (P < 0.01). The appearance of diarrhea was significantly higher in patients with swine-origin influenza compared to those with seasonal influenza (P < 0.005). There was no statistically significant difference in the number of hospitalizations, need for intensive care, assisted ventilation, and mortality rate between the two groups. \u0000Conclusions: Higher prevalence of disease in younger individuals, higher rate of gastrointestinal manifestations and occurrence outside of the epidemic season, were the most important characteristics of swine-origin influenza in comparison to seasonal influenza, in the 2009 pandemic of Qazvin province, Iran. \u0000Keywords: Influenza A Virus, H1N1 Subtype; Epidemiology","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79009898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There are a considerable number of published studies that strongly support the benefits of physical activity in children, and to achieve such benefits, guidelines recommend children to participate in at least 60 minutes of physical activity every day. There is a vicious cycle between low physical activity and increased body mass index.
{"title":"Encouragement to be More Physically Active or to Lessen Sedentary Behavior; Are These Two as the Same?","authors":"K. Hosseinzadeh, S. Niknami, A. Hidarnia","doi":"10.17795/BHS-26201","DOIUrl":"https://doi.org/10.17795/BHS-26201","url":null,"abstract":"There are a considerable number of published studies that strongly support the benefits of physical activity in children, and to achieve such benefits, guidelines recommend children to participate in at least 60 minutes of physical activity every day. There is a vicious cycle between low physical activity and increased body mass index.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76173512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. H. S. Javadi, Zoya Sadeghipour, A. Barikani, M. Javadi
Background: Death from hemorrhage is still the leading cause of maternal mortality. Objectives: The aim of this study was to determine the effect of tranexamic acid on the control of uterine atony during labor. Patients and Methods: A randomized clinical trial was conducted on 90 pregnant women who had uterine atony in Qazvin, during the year 2012. The control group (n = 45) received the routine treatment of uterine atony. The second group (n = 45), in addition to the routine treatments, received 1 gram of tranexamic acid diluted in 100 mL saline of 5% dextrose in water by intravenous infusion within 10 minutes. The amount of blood loss, changes in hemoglobin level, need for surgical intervention and transfusion of blood products and duration of hospitalization were compared between the two groups. Data were analyzed using the chi-square test and t test. Results: Hemoglobin level was 9.9 ± 5.1 in the control group six hours after hemorrhage while it was 8.10 ± 2.1 in the treatment group (P = 0.004). Hemoglobin level was 5.8 ± 4.1 in the control who did not receive transfusion of blood products during the first 24 hours after hemorrhage, while this level was 7.9 ± 4.1 in the treatment group (P = 0.001). The amount of bleeding significantly declined in the intervention group compared to the control group (P < 0.001). Moreover, the need for transfusion of blood products decreased by a third (P < 0.001) while the number of hospitalization days significantly decreased as well (P < 0.04). Conclusions: Tranexamic acid can significantly reduce the rate of postpartum hemorrhage.
{"title":"Tranexamic Acid in the Control of Uterine Atony During Labor","authors":"E. H. S. Javadi, Zoya Sadeghipour, A. Barikani, M. Javadi","doi":"10.17795/BHS-26898","DOIUrl":"https://doi.org/10.17795/BHS-26898","url":null,"abstract":"Background: Death from hemorrhage is still the leading cause of maternal mortality. Objectives: The aim of this study was to determine the effect of tranexamic acid on the control of uterine atony during labor. Patients and Methods: A randomized clinical trial was conducted on 90 pregnant women who had uterine atony in Qazvin, during the year 2012. The control group (n = 45) received the routine treatment of uterine atony. The second group (n = 45), in addition to the routine treatments, received 1 gram of tranexamic acid diluted in 100 mL saline of 5% dextrose in water by intravenous infusion within 10 minutes. The amount of blood loss, changes in hemoglobin level, need for surgical intervention and transfusion of blood products and duration of hospitalization were compared between the two groups. Data were analyzed using the chi-square test and t test. Results: Hemoglobin level was 9.9 ± 5.1 in the control group six hours after hemorrhage while it was 8.10 ± 2.1 in the treatment group (P = 0.004). Hemoglobin level was 5.8 ± 4.1 in the control who did not receive transfusion of blood products during the first 24 hours after hemorrhage, while this level was 7.9 ± 4.1 in the treatment group (P = 0.001). The amount of bleeding significantly declined in the intervention group compared to the control group (P < 0.001). Moreover, the need for transfusion of blood products decreased by a third (P < 0.001) while the number of hospitalization days significantly decreased as well (P < 0.04). Conclusions: Tranexamic acid can significantly reduce the rate of postpartum hemorrhage.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86624556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Talat Dabaghi, M. Shariati, Masoumeh Dadashaliha, M. Bakhshayesh, A. Zargar
dehydrogenase 2171 IU/L and platelet of 53000 mm 3 after 48 hours of the onset of pain, also indicated the possibility of partial HELLP syndrome. However, the results of the differential diagnosis ruled out the presence of Disseminated Intravascular Coagulation (DIC), Thrombotic Thrombocytopenic Purpura (TTP), Systemic Lupus Erythematosus (SLE) and Anti phospholipids syndrome. We terminated her pregnancy due to the above-mentioned diagnoses and postponed the cholecystectomy. Conclusions: Termination of pregnancy was performed as it would save the patient’s life in either deteriorated acute severe pancreatitis or HELLP.
脱氢酶2171 IU/L，血小板53000 mm 3，发病48 h后也提示部分HELLP综合征的可能性。然而，鉴别诊断结果排除了弥散性血管内凝血(DIC)、血栓性血小板减少性紫癜(TTP)、系统性红斑狼疮(SLE)和抗磷脂综合征的存在。由于上述诊断，我们终止了她的妊娠，并推迟了胆囊切除术。结论:无论是恶化的急性重症胰腺炎还是HELLP，终止妊娠都可以挽救患者的生命。
{"title":"Acute Severe Pancreatitis in Pregnancy Masquerading as Partial Hemolysis Elevated Liver enzymes Low Platelet (HELLP) Syndrome","authors":"Talat Dabaghi, M. Shariati, Masoumeh Dadashaliha, M. Bakhshayesh, A. Zargar","doi":"10.17795/BHS-28781","DOIUrl":"https://doi.org/10.17795/BHS-28781","url":null,"abstract":"dehydrogenase 2171 IU/L and platelet of 53000 mm 3 after 48 hours of the onset of pain, also indicated the possibility of partial HELLP syndrome. However, the results of the differential diagnosis ruled out the presence of Disseminated Intravascular Coagulation (DIC), Thrombotic Thrombocytopenic Purpura (TTP), Systemic Lupus Erythematosus (SLE) and Anti phospholipids syndrome. We terminated her pregnancy due to the above-mentioned diagnoses and postponed the cholecystectomy. Conclusions: Termination of pregnancy was performed as it would save the patient’s life in either deteriorated acute severe pancreatitis or HELLP.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89599967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bakhshi, Z. Hassani, M. Tofangchiha, M. Baharvand
histopathologically. Data were analyzed by the Chi-square test and logistic regression by means of the SPSS software version 18. Results: Out of the 129 elderly participants, aged between 60-87 years (mean: 66.71 ± 6.21), males constituted 58.1% (n = 75) of patients and female were 41.9% (n = 54). Normal variations of oral mucosa were observed in 62% (n = 80) of patients, while 44.2% (n = 57) had oral mucosal lesions. Normal variations were significantly associated with smoking (OR = 1.2), while denture wearers were at higher risk of oral pathological lesions (OR = 1.5). Meanwhile, the frequency of normal variations in men was 1.3 folds higher than that of women (P = 0.04). Conclusions: The frequency of normal variations and oral lesions among elderly dental patients is high, and this is significantly associated with smoking, denture wearing and male sex.
{"title":"Frequency of Oral Anatomic Variations and Mucosal Lesions Among a Defined Group of Elderly Dental Patients in Iran","authors":"M. Bakhshi, Z. Hassani, M. Tofangchiha, M. Baharvand","doi":"10.17795/BHS-25758","DOIUrl":"https://doi.org/10.17795/BHS-25758","url":null,"abstract":"histopathologically. Data were analyzed by the Chi-square test and logistic regression by means of the SPSS software version 18. Results: Out of the 129 elderly participants, aged between 60-87 years (mean: 66.71 ± 6.21), males constituted 58.1% (n = 75) of patients and female were 41.9% (n = 54). Normal variations of oral mucosa were observed in 62% (n = 80) of patients, while 44.2% (n = 57) had oral mucosal lesions. Normal variations were significantly associated with smoking (OR = 1.2), while denture wearers were at higher risk of oral pathological lesions (OR = 1.5). Meanwhile, the frequency of normal variations in men was 1.3 folds higher than that of women (P = 0.04). Conclusions: The frequency of normal variations and oral lesions among elderly dental patients is high, and this is significantly associated with smoking, denture wearing and male sex.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77922432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mahshid Saffarpour, A. Peymani, S. Rahrotaban, Marjan Rahmani, M. Ebrahimi
Background: Contamination of dental unit waterlines is a well-known issue in the field of dentistry. Objectives: This study was designed to evaluate contamination of dental unit waterlines by common gram positive and negative bacteria in Qazvin's dental school during the year 2012. Materials and Methods: This survey was performed on 24 dental units routinely used in the dental school of the city of Qazvin. Four samples of 100 mL were obtained in sterile bottles from each unit at different work stages as follows: the beginning of daily activity, after flushing for 30 seconds, following two minutes of flushing, and after the end of daily practice. The number of bacterial colonies was determined for each sample and the mean colony number was calculated. Results: Bacterial contamination was found in all 24 units at the beginning of daily activity with a mean colony count of 17850. The number of colonies decreased to 3250 following 30 seconds of flushing in 16 units and later to 1837 after flushing for two minutes in 13 units. In addition, the number of contaminated units at the end of daily activity was 19 with a mean colony number of 11170. Conclusions: Flushing dental unit waterlines are an effective measure in reducing the number of bacterial contaminants.
{"title":"Evaluation of Bacterial Contamination in Dental Unit Waterlines of Qazvin' Dental School, Iran","authors":"Mahshid Saffarpour, A. Peymani, S. Rahrotaban, Marjan Rahmani, M. Ebrahimi","doi":"10.17795/BHS-26943","DOIUrl":"https://doi.org/10.17795/BHS-26943","url":null,"abstract":"Background: Contamination of dental unit waterlines is a well-known issue in the field of dentistry. Objectives: This study was designed to evaluate contamination of dental unit waterlines by common gram positive and negative bacteria in Qazvin's dental school during the year 2012. Materials and Methods: This survey was performed on 24 dental units routinely used in the dental school of the city of Qazvin. Four samples of 100 mL were obtained in sterile bottles from each unit at different work stages as follows: the beginning of daily activity, after flushing for 30 seconds, following two minutes of flushing, and after the end of daily practice. The number of bacterial colonies was determined for each sample and the mean colony number was calculated. Results: Bacterial contamination was found in all 24 units at the beginning of daily activity with a mean colony count of 17850. The number of colonies decreased to 3250 following 30 seconds of flushing in 16 units and later to 1837 after flushing for two minutes in 13 units. In addition, the number of contaminated units at the end of daily activity was 19 with a mean colony number of 11170. Conclusions: Flushing dental unit waterlines are an effective measure in reducing the number of bacterial contaminants.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86002987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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