K. Crisóstomo-Ayala, M. Hernández de la Torre, M. Pedreño, J. A. Hernandez, C. Perez, E. Bustos, M. Sanchez-Olate, D. Rios
1Centro de Biotecnología, Facultad de Ciencias Forestales, Universidad de Concepción, Concepción, 4070386, Chile 2Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Murcia, E-30100, España 3Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, 4070386, Concepción, Chile 4Grupo de Biotecnología de Frutales, Departamento de Mejora Genética, CEBAS-CSIC, Murcia, E-30100, España
{"title":"Seasonal changes in photosynthesis, phenolic content, antioxidant activity, and anatomy of apical and basal leaves of Aristotelia chilensis","authors":"K. Crisóstomo-Ayala, M. Hernández de la Torre, M. Pedreño, J. A. Hernandez, C. Perez, E. Bustos, M. Sanchez-Olate, D. Rios","doi":"10.32615/bp.2021.052","DOIUrl":"https://doi.org/10.32615/bp.2021.052","url":null,"abstract":"1Centro de Biotecnología, Facultad de Ciencias Forestales, Universidad de Concepción, Concepción, 4070386, Chile 2Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Murcia, E-30100, España 3Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, 4070386, Concepción, Chile 4Grupo de Biotecnología de Frutales, Departamento de Mejora Genética, CEBAS-CSIC, Murcia, E-30100, España","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45954919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Chen, Y. Li, X. Huang, J. Deng, Chunxiao Qu, X. Q. Zhang, B. Huang, Y. Zhang, L. Gong, K. Yu
Terpenoids form the largest class of plant secondary metabolites are very structurally diverse, with more than 50 000 natural products identified (Vattekkatte et al. 2018). They have essential functions in various basic plant processes (e.g., signaling molecules and phytohormones) and myriad roles in plant secondary metabolism, such as the repelling of herbivores, attraction of beneficial organisms, communication between plants, and mediation of complex interactions with the environment (Pichersky and Raguso 2018). Their extensive use in cosmetics, as flavorings, in pharmaceuticals, in the chemical industry, and as biofuel substitutes has made terpenoids indispensable (Pyne et al. 2019). Although terpenoids have different chemical structures, they are biosynthesized from two interconvertible fivecarbon compounds: isopentenyl diphosphate (IPP) and its allylic isomer dimethylallyl diphosphate (DMAPP). These compounds are generated separately by the methylerythritol phosphate (MEP) and mevalonic acid (MVA) pathways in plastids and the cytoplasm, respectively (Vattekkatte et al. 2018). IPP and DMAPP are then condensed head-to-tail by prenyltransferases to produce the terpene precursors
{"title":"Cloning and functional characterization of a terpene synthase gene AlTPS1 from Atractylodes lancea","authors":"L. Chen, Y. Li, X. Huang, J. Deng, Chunxiao Qu, X. Q. Zhang, B. Huang, Y. Zhang, L. Gong, K. Yu","doi":"10.32615/bp.2021.054","DOIUrl":"https://doi.org/10.32615/bp.2021.054","url":null,"abstract":"Terpenoids form the largest class of plant secondary metabolites are very structurally diverse, with more than 50 000 natural products identified (Vattekkatte et al. 2018). They have essential functions in various basic plant processes (e.g., signaling molecules and phytohormones) and myriad roles in plant secondary metabolism, such as the repelling of herbivores, attraction of beneficial organisms, communication between plants, and mediation of complex interactions with the environment (Pichersky and Raguso 2018). Their extensive use in cosmetics, as flavorings, in pharmaceuticals, in the chemical industry, and as biofuel substitutes has made terpenoids indispensable (Pyne et al. 2019). Although terpenoids have different chemical structures, they are biosynthesized from two interconvertible fivecarbon compounds: isopentenyl diphosphate (IPP) and its allylic isomer dimethylallyl diphosphate (DMAPP). These compounds are generated separately by the methylerythritol phosphate (MEP) and mevalonic acid (MVA) pathways in plastids and the cytoplasm, respectively (Vattekkatte et al. 2018). IPP and DMAPP are then condensed head-to-tail by prenyltransferases to produce the terpene precursors","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48916434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1 Anxi College of Tea Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Department of Horticulture and Biotechnology, Chinese Culture University, Taipei 11114, Taiwan 3 Department of Life Sciences and Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, 40227, Taiwan 4 Biodiversity Research Center, Academia Sinica, Taipei 11115, Taiwan
{"title":"Immunogold-labelling localization of chlorophyllase-2at different developmental stages of Pachira macrocarpa leaves","authors":"T. C. Lee, K. Lin, M. Huang, C.-M. Yang","doi":"10.32615/bp.2021.048","DOIUrl":"https://doi.org/10.32615/bp.2021.048","url":null,"abstract":"1 Anxi College of Tea Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Department of Horticulture and Biotechnology, Chinese Culture University, Taipei 11114, Taiwan 3 Department of Life Sciences and Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, 40227, Taiwan 4 Biodiversity Research Center, Academia Sinica, Taipei 11115, Taiwan","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43938958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BackgroundLow temperature is an important factor that influences the ability of winter wheat to safely overwinter. Excessive low temperatures restrict the regrowth of winter wheat, thus decreasing agricultural output. Non-enzymatic expansins, which are related to plant growth, have been reported to respond to drought, salinity, and low temperature stress. We obtained an expansin gene, TaEXPA9, that is induced by low temperature from a transcriptome analysis of ‘Dongnong winter wheat no. 2’—a winter wheat with high cold hardiness—but the expression pattern and function of this gene were unknown. We therefore analyzed the expression patterns of TaEXPA9-A/B/D in D2 in response to different abiotic stresses and exogenous phytohormone treatments in different organs. The entire length of TaEXPA9-A/B/D was obtained, and green fluorescent labeling was used for subcellular localization analysis of TaEXPA9-A/B/D on onion epidermis. The 35S::TaEXPA9-A/B/D expression vector was constructed, and an overexpression transgenic Arabidopsis thaliana line was obtained to examine the effects of the homologs of this expansin on plant growth and low temperature stress resistance. ResultsThe results showed that TaEXPA9-A/B/D transcription significantly increased at 4°C low temperature stress, its expression level was higher in the roots, and TaEXPA9-A/B/D was localized to the cell wall. The roots were well-developed in the overexpression A. thaliana, and the growth-related markers and setting rate were better than in the wild-type. Recovery was stronger in the overexpression plants after frost stress. At 4°C low temperature stress, the antioxidant enzyme activity and osmoregulatory substance content in the TaEXPA9-A/B/D-overexpressing A. thaliana plants were significantly higher than in the wild-type plants, and the degree of membrane lipid peroxidation was lower. ConclusionsIn summary, TaEXPA9-A/B/D participates in the low-temperature stress response and may increase the scavenging of reactive oxygen species caused by low temperature stress through the protective enzyme system. Additionally, TaEXPA9-A/B/D can increase the levels of small molecular organic substances to resist osmotic stress caused by low temperature.
{"title":"Cloning and Functional Analysis of Expansin TaEXPA9 Homologs in Winter Wheat in Frigid Regions","authors":"Ziyi Zhao, Baozhong Hu, Xu Feng, Fenglan Li, Fumeng He, Jiawen Wu, Chongjing Xu, Li Li, Yo. Xu","doi":"10.21203/rs.3.rs-1098291/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-1098291/v1","url":null,"abstract":"\u0000 BackgroundLow temperature is an important factor that influences the ability of winter wheat to safely overwinter. Excessive low temperatures restrict the regrowth of winter wheat, thus decreasing agricultural output. Non-enzymatic expansins, which are related to plant growth, have been reported to respond to drought, salinity, and low temperature stress. We obtained an expansin gene, TaEXPA9, that is induced by low temperature from a transcriptome analysis of ‘Dongnong winter wheat no. 2’—a winter wheat with high cold hardiness—but the expression pattern and function of this gene were unknown. We therefore analyzed the expression patterns of TaEXPA9-A/B/D in D2 in response to different abiotic stresses and exogenous phytohormone treatments in different organs. The entire length of TaEXPA9-A/B/D was obtained, and green fluorescent labeling was used for subcellular localization analysis of TaEXPA9-A/B/D on onion epidermis. The 35S::TaEXPA9-A/B/D expression vector was constructed, and an overexpression transgenic Arabidopsis thaliana line was obtained to examine the effects of the homologs of this expansin on plant growth and low temperature stress resistance. ResultsThe results showed that TaEXPA9-A/B/D transcription significantly increased at 4°C low temperature stress, its expression level was higher in the roots, and TaEXPA9-A/B/D was localized to the cell wall. The roots were well-developed in the overexpression A. thaliana, and the growth-related markers and setting rate were better than in the wild-type. Recovery was stronger in the overexpression plants after frost stress. At 4°C low temperature stress, the antioxidant enzyme activity and osmoregulatory substance content in the TaEXPA9-A/B/D-overexpressing A. thaliana plants were significantly higher than in the wild-type plants, and the degree of membrane lipid peroxidation was lower. ConclusionsIn summary, TaEXPA9-A/B/D participates in the low-temperature stress response and may increase the scavenging of reactive oxygen species caused by low temperature stress through the protective enzyme system. Additionally, TaEXPA9-A/B/D can increase the levels of small molecular organic substances to resist osmotic stress caused by low temperature.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44066342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Z. Jahangir, S. Naz, M. Saleem, M. Khan, A. Younas, Z. Qamar, Q. Ali
Drought, salinity, and frost result in dehydration of plant cells. The dehydration signals in plants trigger the synthesis of dehydration-induced cellular proteins (dehydrins) which were first observed in maize and barley in 1989 as reported in Yao et al. (2005) and Ali et al. (2014, 2016). Dehydrins belong to a multi-protein family called late embryogenesis abundant (LEA) proteins group-2 (Puhakainen et al. 2004, Yang et al. 2012). The dehydrins are located in the cytoplasm, nucleus, mitochondria, and chloroplast (Xu et al. 2005). Further, dehydrins have been found in the endoplasmic reticulum, plasma membrane, and tonoplasts (Close et al. 1993, Close 1996). The extensive accumulation of dehydrins has been observed in the plant embryos during later developmental stages, just like other LEA proteins (Hanin et al. 2011). The dehydrins accumulate extensively in all vegetative tissues when plants are subjected to environmental stresses that may cause the dehydration of cells like osmotic stress, drought, salinity, and heat (Hanin et al. 2011). The dehydrins are hydrophilic and thermostable
干旱、盐度和霜冻导致植物细胞脱水。植物中的脱水信号触发脱水诱导的细胞蛋白(dehydrins)的合成，Yao等人(2005)和Ali等人(2014,2016)于1989年首次在玉米和大麦中观察到这一现象。脱水蛋白属于一个多蛋白家族，称为晚期胚胎发生丰富蛋白(LEA)蛋白群-2 (Puhakainen et al. 2004, Yang et al. 2012)。脱水剂位于细胞质、细胞核、线粒体和叶绿体中(Xu et al. 2005)。此外，在内质网、质膜和细胞体中也发现了脱水剂(Close et al. 1993, Close 1996)。与其他LEA蛋白一样，在发育后期的植物胚胎中，已经观察到脱水蛋白的大量积累(Hanin et al. 2011)。当植物受到渗透胁迫、干旱、盐度和高温等可能导致细胞脱水的环境胁迫时，脱水剂在所有营养组织中广泛积累(Hanin et al. 2011)。脱水剂具有亲水性和耐热性
{"title":"Molecular characterization and expression studiesof Eucalyptus globulus stress-responsive gene DHN-10","authors":"G. Z. Jahangir, S. Naz, M. Saleem, M. Khan, A. Younas, Z. Qamar, Q. Ali","doi":"10.32615/bp.2019.107","DOIUrl":"https://doi.org/10.32615/bp.2019.107","url":null,"abstract":"Drought, salinity, and frost result in dehydration of plant cells. The dehydration signals in plants trigger the synthesis of dehydration-induced cellular proteins (dehydrins) which were first observed in maize and barley in 1989 as reported in Yao et al. (2005) and Ali et al. (2014, 2016). Dehydrins belong to a multi-protein family called late embryogenesis abundant (LEA) proteins group-2 (Puhakainen et al. 2004, Yang et al. 2012). The dehydrins are located in the cytoplasm, nucleus, mitochondria, and chloroplast (Xu et al. 2005). Further, dehydrins have been found in the endoplasmic reticulum, plasma membrane, and tonoplasts (Close et al. 1993, Close 1996). The extensive accumulation of dehydrins has been observed in the plant embryos during later developmental stages, just like other LEA proteins (Hanin et al. 2011). The dehydrins accumulate extensively in all vegetative tissues when plants are subjected to environmental stresses that may cause the dehydration of cells like osmotic stress, drought, salinity, and heat (Hanin et al. 2011). The dehydrins are hydrophilic and thermostable","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45580227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Zhao, Y. Wang, K. Gao, Y. Zhang, Y. Shi, Y. Miao
Low temperatures are one of the most significant abiotic factors limiting crop growth and development with increasing losses in the agricultural sector resulting from early frosts in the fall, freezing temperatures in the winter, and sudden cold spells in the late spring (Hu et al. 2010, Cooper et al. 2018, Wang et al. 2019b). Sudden chilling causes a number of serious metabolic disorders in plants, with one of the most significant being a decrease in the rate of photosynthesis. Even if more suitable temperatures prevail immediately after a chilling event, it still takes
{"title":"Melatonin alleviates photoinhibition in cucumber seedlings by modulating partitioning of absorbed excitation energy in photosystem Ⅱ","authors":"H. Zhao, Y. Wang, K. Gao, Y. Zhang, Y. Shi, Y. Miao","doi":"10.32615/bp.2021.039","DOIUrl":"https://doi.org/10.32615/bp.2021.039","url":null,"abstract":"Low temperatures are one of the most significant abiotic factors limiting crop growth and development with increasing losses in the agricultural sector resulting from early frosts in the fall, freezing temperatures in the winter, and sudden cold spells in the late spring (Hu et al. 2010, Cooper et al. 2018, Wang et al. 2019b). Sudden chilling causes a number of serious metabolic disorders in plants, with one of the most significant being a decrease in the rate of photosynthesis. Even if more suitable temperatures prevail immediately after a chilling event, it still takes","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49601873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Hou, Y. Xu, Z. Wang, F. Chen, L. Yuan, S. Zhu, G. Shan, C. Wang
Received 3 April 2021, last revision 7 August 2021, accepted 1 September 2021. Abbreviations: ABS/CSo absorption flux per cross section (CS) (at t = 0); ABS/RC radiation energy absorbed by RC; Chl chlorophyll; DIo/CSo dissipated energy flux per cross section (CS) (at t = 0); DIo/RC light energy dissipated by RC (at t = 0); ETo/CSo electron transport flux per cross section (CS) (at t = 0); ETo/RC the RC unit of the reaction center captures the energy used for electron transport (at t = 0); Fm maximal chlorophyll fluorescence measured in the dark-adapted state during the application of a saturating radiation pulse; Fo minimal chlorophyll fluorescence measured in the dark-adapted state when all PS II RCs are open; Fv/Fm maximum quantum yield of PS II photochemistry measured in the dark-adapted state; Fv/F0 efficiency of the water-splitting complex on the donor side of PS II; GA3 gibberellic acid; MDA malondialdehyde; PN net photosynthetic rate; PP333 paclobutrazol; PS photosystem; LCP light compensation point; RC reaction center of PS II; TBA thiobarbituric acid; TCA trichloroacetic acid; TRo/CSo trapped energy flux per cross section (CS) (at t = 0); TRo/RC RC captures energy used to restore QA (at t = 0). Acknowledgements: This work was supported by the Natural science foundation of higher education institutions of Anhui province, China (KJ2018A0155). +These authors contributed equally. Conflict of interest: The authors declare that they have no conflict of interest. Abstract
收到2021年4月3日，最后修订2021年8月7日，接受2021年9月1日。缩写:ABS/CSo每横截面吸收通量(CS) (at t = 0);ABS/RC被RC吸收的辐射能;的背影叶绿素;DIo/CSo每截面耗散能量通量(CS) (t = 0时);DIo/RC光能被RC耗散(t = 0时);ETo/CSo每横截面电子输运通量(CS) (t = 0);ETo/RC反应中心的RC单元捕获用于电子传递的能量(t = 0时);饱和辐射脉冲在暗适应状态下的最大叶绿素荧光测量当所有PS II RCs都打开时，在暗适应状态下测量的最小叶绿素荧光;暗适应状态下PSⅱ光化学的最大量子产率Fv/FmPSⅱ供体侧水裂解配合物的Fv/F0效率;GA3赤霉素酸;MDA丙二醛;净光合速率;PP333证明;PS光系统;LCP光补偿点;PSⅱ的RC反应中心;TBA硫巴比妥酸;三氯乙酸;每横截面TRo/CSo捕获能量通量(CS) (t = 0);TRo/RC RC捕获能量用于恢复QA (at t = 0)。感谢:本工作得到中国安徽省高等学校自然科学基金(KJ2018A0155)的支持。这些作者贡献均等。利益冲突:作者声明他们没有利益冲突。摘要
{"title":"Exogenous paclobutrazol can relieve the low irradiance stress in Capsicum annuum seedlings","authors":"J. Hou, Y. Xu, Z. Wang, F. Chen, L. Yuan, S. Zhu, G. Shan, C. Wang","doi":"10.32615/bp.2021.055","DOIUrl":"https://doi.org/10.32615/bp.2021.055","url":null,"abstract":"Received 3 April 2021, last revision 7 August 2021, accepted 1 September 2021. Abbreviations: ABS/CSo absorption flux per cross section (CS) (at t = 0); ABS/RC radiation energy absorbed by RC; Chl chlorophyll; DIo/CSo dissipated energy flux per cross section (CS) (at t = 0); DIo/RC light energy dissipated by RC (at t = 0); ETo/CSo electron transport flux per cross section (CS) (at t = 0); ETo/RC the RC unit of the reaction center captures the energy used for electron transport (at t = 0); Fm maximal chlorophyll fluorescence measured in the dark-adapted state during the application of a saturating radiation pulse; Fo minimal chlorophyll fluorescence measured in the dark-adapted state when all PS II RCs are open; Fv/Fm maximum quantum yield of PS II photochemistry measured in the dark-adapted state; Fv/F0 efficiency of the water-splitting complex on the donor side of PS II; GA3 gibberellic acid; MDA malondialdehyde; PN net photosynthetic rate; PP333 paclobutrazol; PS photosystem; LCP light compensation point; RC reaction center of PS II; TBA thiobarbituric acid; TCA trichloroacetic acid; TRo/CSo trapped energy flux per cross section (CS) (at t = 0); TRo/RC RC captures energy used to restore QA (at t = 0). Acknowledgements: This work was supported by the Natural science foundation of higher education institutions of Anhui province, China (KJ2018A0155). +These authors contributed equally. Conflict of interest: The authors declare that they have no conflict of interest. Abstract","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46007904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Yu, Yongchao Ke, Kecheng Zhang, M. Yan, H. Jin, Y. Chen, J. Zhang
Received 6 February 2021, last revision 2 June 2021, accepted 9 June 2021. Abbreviations: F-1,6-P2 fructose-1,6-bisphosphate; F-2,6-P2 fructose-2,6-bisphosphate; F-6-P fructose-6-phosphate; FBP fructose1,6-bisphosphatase; G-1-P glucose-1-phosphate; HP3 Huapei3; Mr molecular mass; PAGE polyacrylamide gel electrophoresis; PFK ATP-dependent phosphofructokinase; PFP pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase; pI isoelectric point; PPP pentose phosphate pathway; SBP -sedoheptulose-1,7-bisphosphatase; TCA tricarboxylic acid; TGM thousand-grain mass; TPM transcripts per million. Acknowledgements: This research was supported by the National Program on Key Basic Research Project (2016YFD0100500). We thank Z.Y. Mao for assistance in propagating the wheat mutant population in the field station and all colleagues at the Lab Center of the School of Life Sciences of Nantong University for assistance in the use of instruments. Conflict of interest: The authors declare that they have no conflict of interest. Abstract
{"title":"Identification of three gene families coordinating the conversion between fructose-6-phosphate and fructose-1,6-bisphosphate in wheat","authors":"C. Yu, Yongchao Ke, Kecheng Zhang, M. Yan, H. Jin, Y. Chen, J. Zhang","doi":"10.32615/bp.2021.035","DOIUrl":"https://doi.org/10.32615/bp.2021.035","url":null,"abstract":"Received 6 February 2021, last revision 2 June 2021, accepted 9 June 2021. Abbreviations: F-1,6-P2 fructose-1,6-bisphosphate; F-2,6-P2 fructose-2,6-bisphosphate; F-6-P fructose-6-phosphate; FBP fructose1,6-bisphosphatase; G-1-P glucose-1-phosphate; HP3 Huapei3; Mr molecular mass; PAGE polyacrylamide gel electrophoresis; PFK ATP-dependent phosphofructokinase; PFP pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase; pI isoelectric point; PPP pentose phosphate pathway; SBP -sedoheptulose-1,7-bisphosphatase; TCA tricarboxylic acid; TGM thousand-grain mass; TPM transcripts per million. Acknowledgements: This research was supported by the National Program on Key Basic Research Project (2016YFD0100500). We thank Z.Y. Mao for assistance in propagating the wheat mutant population in the field station and all colleagues at the Lab Center of the School of Life Sciences of Nantong University for assistance in the use of instruments. Conflict of interest: The authors declare that they have no conflict of interest. Abstract","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41398335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Luo, X.-Y. Liu, Y.-J. Xue, X.-Y. Cao, J.‐J. Liu, M. Geng
Heat stress limits wheat production and trehalose can improve stress tolerance. How trehalose affects wheat respiration is unclear. In this study, we investigated the effects of exogenous trehalose on the respiration of wheat seedlings during heat stress and the subsequent recovery period. Trehalose pretreatment significantly increased the expression of the alternative oxidase genes AOX1a and AOX1c under heat stress, indicating that trehalose pretreatment increased the capacity of the alternative respiration pathway (AP) in response to heat stress. Trehalose pretreatment also enhanced the activity of the malate-oxaloacetate (Mal-OAA) shuttle and ameliorated the decrease in photosynthetic activity caused by heat stress. However, when the AP was inhibited by salicylhydroxamic acid under heat stress, both Mal-OAA shuttle activity and photosynthetic efficiency were substantially reduced in the control and trehalose pretreatment groups. In addition, trehalose pretreatment helped to maintain inner mitochondrial respiratory activity and the activity of Complex II during heat stress, particularly the coupling of oxidative phosphorylation with the Complex II electron transport chain, thereby mitigating heat-related damage to the cytochrome pathway (CP). Taken together, these results suggest that exogenous trehalose enhanced the AP and reduced damage to the CP under heat stress in wheat seedlings, thus maintaining cellular energy metabolism. Up-regulation of the AP by trehalose pretreatment may improve the heat tolerance of wheat seedlings by dissipating excess reducing equivalents transported through the Mal-OAA shuttle, thereby protecting photosynthetic performance.
{"title":"Respiration responses of wheat seedlings to treatment with trehalose under heat stress","authors":"Y. Luo, X.-Y. Liu, Y.-J. Xue, X.-Y. Cao, J.‐J. Liu, M. Geng","doi":"10.32615/bp.2021.025","DOIUrl":"https://doi.org/10.32615/bp.2021.025","url":null,"abstract":"Heat stress limits wheat production and trehalose can improve stress tolerance. How trehalose affects wheat respiration is unclear. In this study, we investigated the effects of exogenous trehalose on the respiration of wheat seedlings during heat stress and the subsequent recovery period. Trehalose pretreatment significantly increased the expression of the alternative oxidase genes AOX1a and AOX1c under heat stress, indicating that trehalose pretreatment increased the capacity of the alternative respiration pathway (AP) in response to heat stress. Trehalose pretreatment also enhanced the activity of the malate-oxaloacetate (Mal-OAA) shuttle and ameliorated the decrease in photosynthetic activity caused by heat stress. However, when the AP was inhibited by salicylhydroxamic acid under heat stress, both Mal-OAA shuttle activity and photosynthetic efficiency were substantially reduced in the control and trehalose pretreatment groups. In addition, trehalose pretreatment helped to maintain inner mitochondrial respiratory activity and the activity of Complex II during heat stress, particularly the coupling of oxidative phosphorylation with the Complex II electron transport chain, thereby mitigating heat-related damage to the cytochrome pathway (CP). Taken together, these results suggest that exogenous trehalose enhanced the AP and reduced damage to the CP under heat stress in wheat seedlings, thus maintaining cellular energy metabolism. Up-regulation of the AP by trehalose pretreatment may improve the heat tolerance of wheat seedlings by dissipating excess reducing equivalents transported through the Mal-OAA shuttle, thereby protecting photosynthetic performance.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45060838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Liang, Y. Cao, D. Wang, Y. Peng, Yan Zhang, Zhenyu Li
Spermine (SPM) is involved in response to abiotic stress in plants, but the potential role of SPM in regulating senescence has not been well documented. Objectives of this study were to examine the effect of changes in endogenous polyamines (PAs) by SPM application on improving heat tolerance of creeping bentgrass (Agrostis stolonifera) and explore the SPM-regulated senescence associated with alterations of water and oxidative balance, photosynthesis, and heat shock proteins under heat stress. The results showed that persistent high temperature caused severe oxidative damage and significant decreases in chlorophyll (Chl) content, photosynthetic efficiency, and leaf water content leading to premature senescence in creeping bentgrass, as reflected by a significant upregulation of transcriptions of senescence-associated genes (AsSAG39, Ash36, and Asl20). The improvement of endogenous spermidine (SPD) and SPM content induced by SPM application could significantly alleviate heat stress damage to creeping bentgrass through maintaining higher Chl content, net photosynthetic rate, photochemical efficiency, and performance index on absorption basis, promoting osmotic adjustment ability and antioxidant enzyme (superoxid dismutase, catalase, peroxidase, and ascorbate peroxidase) activities to enhance the scavenging capacity of reactive oxygen species, and upregulating transcriptions of heat shock protein (HSP) genes (HSP90-5, HSP90.1-b1, HSP82, HSP70, HSP26.7, HSP17.8, and HSP12) helping to maintain normal synthesis and functions of proteins under high temperature stress, thereby delaying heat-induced leaf senescence. These findings reveal an important role of PAs in regulating senescence in perennial plants exposed to a high temperature environment.
{"title":"Spermine alleviates heat-induced senescence in creeping bentgrass by regulating water and oxidative balance, photosynthesis, and heat shock proteins","authors":"L. Liang, Y. Cao, D. Wang, Y. Peng, Yan Zhang, Zhenyu Li","doi":"10.32615/BP.2021.008","DOIUrl":"https://doi.org/10.32615/BP.2021.008","url":null,"abstract":"Spermine (SPM) is involved in response to abiotic stress in plants, but the potential role of SPM in regulating senescence has not been well documented. Objectives of this study were to examine the effect of changes in endogenous polyamines (PAs) by SPM application on improving heat tolerance of creeping bentgrass (Agrostis stolonifera) and explore the SPM-regulated senescence associated with alterations of water and oxidative balance, photosynthesis, and heat shock proteins under heat stress. The results showed that persistent high temperature caused severe oxidative damage and significant decreases in chlorophyll (Chl) content, photosynthetic efficiency, and leaf water content leading to premature senescence in creeping bentgrass, as reflected by a significant upregulation of transcriptions of senescence-associated genes (AsSAG39, Ash36, and Asl20). The improvement of endogenous spermidine (SPD) and SPM content induced by SPM application could significantly alleviate heat stress damage to creeping bentgrass through maintaining higher Chl content, net photosynthetic rate, photochemical efficiency, and performance index on absorption basis, promoting osmotic adjustment ability and antioxidant enzyme (superoxid dismutase, catalase, peroxidase, and ascorbate peroxidase) activities to enhance the scavenging capacity of reactive oxygen species, and upregulating transcriptions of heat shock protein (HSP) genes (HSP90-5, HSP90.1-b1, HSP82, HSP70, HSP26.7, HSP17.8, and HSP12) helping to maintain normal synthesis and functions of proteins under high temperature stress, thereby delaying heat-induced leaf senescence. These findings reveal an important role of PAs in regulating senescence in perennial plants exposed to a high temperature environment.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41650185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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