Pub Date : 2023-02-03DOI: 10.14393/bj-v39n0a2023-62854
Cíntia Gonçalves Guimarães, Caroline Salezzi Bonfá, A. R. Evangelista, A. S. Santos, L. Pantoja, A. G. Guimarães, M. A. M. Ferreira
Elephant grass is indicated for silage production but requires additives to increase dry matter content because it reduces the production of effluents, potentially improves the fermentation pattern, and preserves the nutrients of the silage. This study aimed to evaluate the effects of including macaúba cake in elephant grass ensilage on dry matter content, lactic acid bacteria population, lactic acid production, pH values, losses by gases and effluents, and dry matter recovery. The experimental design was completely randomized, in a 3x6 factorial scheme, with three levels of inclusion of macaúba cake (0, 10, and 20%) and six opening times (1, 5, 10, 20, 40, and 60 days after ensilage), with four repetitions. Macaúba cake was an effective moisture-absorbing additive, increasing dry matter content, lactic acid bacteria population, and lactic acid content and reducing the pH. The losses by effluents and gases decreased, and dry matter recovery increased with the addition of this biodiesel co-product. The 20% level of inclusion of macaúba cake in elephant grass ensilage allowed for better preserving the ensiled material.
{"title":"Fermentative losses and lactic acid content of elephant grass silages added with macaúba cake","authors":"Cíntia Gonçalves Guimarães, Caroline Salezzi Bonfá, A. R. Evangelista, A. S. Santos, L. Pantoja, A. G. Guimarães, M. A. M. Ferreira","doi":"10.14393/bj-v39n0a2023-62854","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62854","url":null,"abstract":"Elephant grass is indicated for silage production but requires additives to increase dry matter content because it reduces the production of effluents, potentially improves the fermentation pattern, and preserves the nutrients of the silage. This study aimed to evaluate the effects of including macaúba cake in elephant grass ensilage on dry matter content, lactic acid bacteria population, lactic acid production, pH values, losses by gases and effluents, and dry matter recovery. The experimental design was completely randomized, in a 3x6 factorial scheme, with three levels of inclusion of macaúba cake (0, 10, and 20%) and six opening times (1, 5, 10, 20, 40, and 60 days after ensilage), with four repetitions. Macaúba cake was an effective moisture-absorbing additive, increasing dry matter content, lactic acid bacteria population, and lactic acid content and reducing the pH. The losses by effluents and gases decreased, and dry matter recovery increased with the addition of this biodiesel co-product. The 20% level of inclusion of macaúba cake in elephant grass ensilage allowed for better preserving the ensiled material.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66670658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.14393/bj-v39n0a2023-61040
Neuza Helena Carvalho de Oliveira, A. P. Coelho, F. C. Meirelles, Ancelmo Cazuza Neto, L. B. Lemos
The aim was to evaluate the agronomic and qualitative attributes of early-cycle common bean cultivars with different grains types grains in response to top-dressing nitrogen (N) doses. The experiment was carried out using a randomized block design, in a split-plot scheme, with 4 replicates. The plots consisted of the cultivars IAC Nuance, IAC 1849 Polaco and IAC Veloz, with speckled, Carioca and black grains, respectively. The subplots were formed by N doses applied as top-dressing: 0 kg ha-1, 60 kg ha-1 (applied in the stage of third trifoliate leaf), 120 kg ha-1 (1/2 applied at third trifoliate leaf stage + 1/2 applied at the floral bud stage) and 180 kg ha-1 (1/3 applied at the first trifoliate leaf stage + 1/3 applied at the third trifoliate leaf stage + 1/3 applied at the floral bud stage). IAC Veloz stood out for grain yield, showing the highest grain yield in the lowest N doses, being classified as efficient to the use of N. The cultivars IAC Nuance and IAC 1849 Polaco reached maximum yields with 155 and 163 kg ha-1 of N. The IAC Nuance was the most responsive, increasing grain yield by up to 25.3% due to nitrogen fertilization. Increasing N doses applied as top-dressing increased the sieve yield and crude protein content of the common bean cultivars, with IAC Nuance standing out. The cultivars showed good grain quality, and IAC 1849 Polaco and IAC Veloz had the shortest cooking time and IAC Veloz also had the fastest hydration.
目的是评估具有不同谷物类型的早周期普通大豆品种对追肥氮（N）剂量的响应的农艺和质量属性。该实验采用随机区组设计，采用分裂图方案，4个重复。该地块由品种IAC Nuance、IAC 1849 Polaco和IAC Veloz组成，分别带有斑点、Carioca和黑色颗粒。通过施用N剂量作为追肥形成子地块：0 kg ha-1、60 kg ha-1（施用于第三三叶阶段）、120 kg ha-1，施用于第三叶阶段的1/2+施用于花蕾阶段的1/2）和180 kg ha-1。IAC Veloz在籽粒产量方面表现突出，在最低氮剂量下表现出最高的籽粒产量，被归类为对氮的有效利用。品种IAC Nuance和IAC 1849 Polaco分别以155和163 kg ha-1的氮达到最高产量。增加追肥施氮量可提高普通大豆品种的筛产量和粗蛋白含量，其中IAC Nuance表现突出。品种表现出良好的籽粒品质，其中IAC 1849 Polaco和IAC Veloz的蒸煮时间最短，IAC Veluz的水合作用也最快。
{"title":"Response of early-cycle common bean cultivars with carioca, black and speckled grains to top-dressing nitrogen fertilization","authors":"Neuza Helena Carvalho de Oliveira, A. P. Coelho, F. C. Meirelles, Ancelmo Cazuza Neto, L. B. Lemos","doi":"10.14393/bj-v39n0a2023-61040","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-61040","url":null,"abstract":"The aim was to evaluate the agronomic and qualitative attributes of early-cycle common bean cultivars with different grains types grains in response to top-dressing nitrogen (N) doses. The experiment was carried out using a randomized block design, in a split-plot scheme, with 4 replicates. The plots consisted of the cultivars IAC Nuance, IAC 1849 Polaco and IAC Veloz, with speckled, Carioca and black grains, respectively. The subplots were formed by N doses applied as top-dressing: 0 kg ha-1, 60 kg ha-1 (applied in the stage of third trifoliate leaf), 120 kg ha-1 (1/2 applied at third trifoliate leaf stage + 1/2 applied at the floral bud stage) and 180 kg ha-1 (1/3 applied at the first trifoliate leaf stage + 1/3 applied at the third trifoliate leaf stage + 1/3 applied at the floral bud stage). IAC Veloz stood out for grain yield, showing the highest grain yield in the lowest N doses, being classified as efficient to the use of N. The cultivars IAC Nuance and IAC 1849 Polaco reached maximum yields with 155 and 163 kg ha-1 of N. The IAC Nuance was the most responsive, increasing grain yield by up to 25.3% due to nitrogen fertilization. Increasing N doses applied as top-dressing increased the sieve yield and crude protein content of the common bean cultivars, with IAC Nuance standing out. The cultivars showed good grain quality, and IAC 1849 Polaco and IAC Veloz had the shortest cooking time and IAC Veloz also had the fastest hydration.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49554099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.14393/bj-v39n0a2023-61615
A. E. A. Brito, Kerolém Prícila Sousa Cardoso, Thays Correa Costa, J. T. Martins, Liliane Corrêa Machado, G. Nogueira, S. S. Conceição, J. T. D. Oliveira, P. A. Silva, Raimundo Thiago Lima da Silva, C. F. O. Oliveira Neto
Due to rainfall and high temperatures, the Amazonian soil undergoes changes in its source material and leaching of base cations. This results in deep, infertile, and acidic soil. Aluminum present in acidic soil impairs plant growth and development by inhibiting root formation, enzymatic reactions, absorption, transport, and nutrient utilization. This study aimed to evaluate the effects of aluminum dosage on the metabolism of the oil palm Elaeis guineensis Jacq. The study was conducted in a greenhouse at the Federal Rural University of Amazonia. The experimental design was randomized, with five replications, in which dosages of 0, 10, 20, 30, and 40 mg L-1 aluminum chloride (AlCl3.6H2O) were administered. Electrolyte leakage, nitrate, nitrate reductase, free ammonium, soluble amino acids, proline content, and soluble proteins were analyzed in the leaves and roots of the oil palm. The highest concentration of aluminum was found in the roots. AlCl3 treatment at 40 mg L-1 increased electrolyte leakage, nitrate, ammonium, and proline concentrations in the roots, and amino acid concentrations in both the leaves and roots. Furthermore, a decrease in nitrate reductase enzyme activity was observed in the roots. This study demonstrates that the oil palm has mechanisms of tolerance to aluminum toxicity.
{"title":"The effect of aluminum treatment on metabolism in oil palm seedlings","authors":"A. E. A. Brito, Kerolém Prícila Sousa Cardoso, Thays Correa Costa, J. T. Martins, Liliane Corrêa Machado, G. Nogueira, S. S. Conceição, J. T. D. Oliveira, P. A. Silva, Raimundo Thiago Lima da Silva, C. F. O. Oliveira Neto","doi":"10.14393/bj-v39n0a2023-61615","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-61615","url":null,"abstract":"Due to rainfall and high temperatures, the Amazonian soil undergoes changes in its source material and leaching of base cations. This results in deep, infertile, and acidic soil. Aluminum present in acidic soil impairs plant growth and development by inhibiting root formation, enzymatic reactions, absorption, transport, and nutrient utilization. This study aimed to evaluate the effects of aluminum dosage on the metabolism of the oil palm Elaeis guineensis Jacq. The study was conducted in a greenhouse at the Federal Rural University of Amazonia. The experimental design was randomized, with five replications, in which dosages of 0, 10, 20, 30, and 40 mg L-1 aluminum chloride (AlCl3.6H2O) were administered. Electrolyte leakage, nitrate, nitrate reductase, free ammonium, soluble amino acids, proline content, and soluble proteins were analyzed in the leaves and roots of the oil palm. The highest concentration of aluminum was found in the roots. AlCl3 treatment at 40 mg L-1 increased electrolyte leakage, nitrate, ammonium, and proline concentrations in the roots, and amino acid concentrations in both the leaves and roots. Furthermore, a decrease in nitrate reductase enzyme activity was observed in the roots. This study demonstrates that the oil palm has mechanisms of tolerance to aluminum toxicity.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48342886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.14393/bj-v39n0a2023-62994
Renan Krupok Matias, Camilla Nascimento Brito, R. Resende, Jovan Martins Rios, Gabriel Mendes Santana, G. M. D. Oliveira, F. Venturoli
African mahogany species (Khaya spp.) have proven to be promising in the Brazilian forestry scenario, replacing native mahogany owing to their medium-fast growth and relevant timber value. This study aimed to carry out forest inventory and assessments of a Khaya grandifoliola plantation in the first years after planting, test hypsometric models to describe tree growth, and identify the maximum commercial stem yield (i.e., greater than 6 m in height). The stand was located in the municipality of Piracanjuba (GO), where seedlings of seed origin were used. Twenty random plots with a 15 m radius were allocated, and the total height (HT), stem height (HS), diameter at breast height (DBH), crown area, and forest canopy were measured. Four hypsometric models were employed in this study. The best equation was selected based on determination coefficients and standard errors. Further, the models were cross-validated to evaluate predictability and bias. At four years of planting, the largest class of HS was found to range from 3.1 to 4.1 m, and most trees had a DBH ranging from 0.084 to 0.126 m. The percentage of trees with stems > 6 m was 8.35%. The linear model ensured more consistent results for estimating HT, while the quadratic and Weibull models led to more consistent results for HS. By using models, stem measurements can be measured based on DBH, ultimately aiding the selection of stem management strategies for the growth of forests with greater commercial value.
{"title":"Biometric assessment of early stem growth at a commercial stand of African mahogany (Khaya grandifoliola)","authors":"Renan Krupok Matias, Camilla Nascimento Brito, R. Resende, Jovan Martins Rios, Gabriel Mendes Santana, G. M. D. Oliveira, F. Venturoli","doi":"10.14393/bj-v39n0a2023-62994","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62994","url":null,"abstract":"African mahogany species (Khaya spp.) have proven to be promising in the Brazilian forestry scenario, replacing native mahogany owing to their medium-fast growth and relevant timber value. This study aimed to carry out forest inventory and assessments of a Khaya grandifoliola plantation in the first years after planting, test hypsometric models to describe tree growth, and identify the maximum commercial stem yield (i.e., greater than 6 m in height). The stand was located in the municipality of Piracanjuba (GO), where seedlings of seed origin were used. Twenty random plots with a 15 m radius were allocated, and the total height (HT), stem height (HS), diameter at breast height (DBH), crown area, and forest canopy were measured. Four hypsometric models were employed in this study. The best equation was selected based on determination coefficients and standard errors. Further, the models were cross-validated to evaluate predictability and bias. At four years of planting, the largest class of HS was found to range from 3.1 to 4.1 m, and most trees had a DBH ranging from 0.084 to 0.126 m. The percentage of trees with stems > 6 m was 8.35%. The linear model ensured more consistent results for estimating HT, while the quadratic and Weibull models led to more consistent results for HS. By using models, stem measurements can be measured based on DBH, ultimately aiding the selection of stem management strategies for the growth of forests with greater commercial value.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44940521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.14393/bj-v39n0a2023-63126
M. Guimarães, L. Dias, C. D. Almeida, C. A. S. Souza, Odimar Ferreira Almeida, T. R. Corrêa
The cocoa and chocolate production chain involves US$60 billion annually and three million farmers around the world, in an area exceeding nine million hectares. The use of wild germplasm will enable to generate new disease- and pest-resistant cultivars and ability to adapt to changing environments. Here we evaluated 145 cocoa accessions, originated from nine Amazonian basins, based on eight fruit traits. Univariate anova showed significant differences (p<0.05) for all traits. For seven traits, the variance component within basins was higher (81.5%, on average). Therefore, it is recommended that the collection of wild accessions prioritize a larger number of plants from a few populations of the most divergent basins. The multivariate analyses revealed a greater divergence between the Ji-Paraná-RO and Solimões/Amazonas-PA basins (27.69) and a greater similarity between Alien clones-PER and Solimões/Amazonas-AM (0.66) in relation to their populations. They also revealed that the accessions differentiation occurred according to the river basin system. These results allowed elucidate the genetic structure and distribution of cacao populations. In addition, strengthen the importance of collecting and conserving germplasm to preserve genetic resources.
{"title":"Genetic variability of cocoa from the amazon basin: implications for genetic conservation","authors":"M. Guimarães, L. Dias, C. D. Almeida, C. A. S. Souza, Odimar Ferreira Almeida, T. R. Corrêa","doi":"10.14393/bj-v39n0a2023-63126","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63126","url":null,"abstract":"The cocoa and chocolate production chain involves US$60 billion annually and three million farmers around the world, in an area exceeding nine million hectares. The use of wild germplasm will enable to generate new disease- and pest-resistant cultivars and ability to adapt to changing environments. Here we evaluated 145 cocoa accessions, originated from nine Amazonian basins, based on eight fruit traits. Univariate anova showed significant differences (p<0.05) for all traits. For seven traits, the variance component within basins was higher (81.5%, on average). Therefore, it is recommended that the collection of wild accessions prioritize a larger number of plants from a few populations of the most divergent basins. The multivariate analyses revealed a greater divergence between the Ji-Paraná-RO and Solimões/Amazonas-PA basins (27.69) and a greater similarity between Alien clones-PER and Solimões/Amazonas-AM (0.66) in relation to their populations. They also revealed that the accessions differentiation occurred according to the river basin system. These results allowed elucidate the genetic structure and distribution of cacao populations. In addition, strengthen the importance of collecting and conserving germplasm to preserve genetic resources.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41852666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.14393/bj-v39n0a2023-63133
Yang Yang, Y. Fu, Qiupu Yang, Chen Li
Tillering onion is a herbaceous plant belonging to the Liliaceae family. We cloned the cDNAs of the actin gene (AcACT, GenBank: MF919598) of tillering onion using rapid amplification of the cDNA ends. The full-length cDNA of AcACT was 1,357 bp long with an open reading frame of 1,131 bp encoding 376 amino acids. The amino acid sequence of AcACT shared > 96% similarity with the amino acid sequences of other ACTs and was found (by means of phylogenetic tree analysis) to be closely related to those of Ananas comosus and Papaver somniferum. AcACT expressions showed no significant differences (p > 0.01) in two cultivars L-SH and L-SY over three growth periods and under suitable conditions, low temperature, and short-day conditions. In addition, AcACT was used as an internal reference gene to analyse the expression of the alliinase gene (AcALL). AcALL expression trends in the roots, stems and leaves were consistent with those of diallyl disulphide and diallyl trisulphide. Thus, AcACT is highly conserved and can be used as a suitable internal reference gene when analysing gene expression in tillering onion.
{"title":"Cloning the full-length CDNA of actin gene and analysing alliinase gene expression in tillering onion","authors":"Yang Yang, Y. Fu, Qiupu Yang, Chen Li","doi":"10.14393/bj-v39n0a2023-63133","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63133","url":null,"abstract":"Tillering onion is a herbaceous plant belonging to the Liliaceae family. We cloned the cDNAs of the actin gene (AcACT, GenBank: MF919598) of tillering onion using rapid amplification of the cDNA ends. The full-length cDNA of AcACT was 1,357 bp long with an open reading frame of 1,131 bp encoding 376 amino acids. The amino acid sequence of AcACT shared > 96% similarity with the amino acid sequences of other ACTs and was found (by means of phylogenetic tree analysis) to be closely related to those of Ananas comosus and Papaver somniferum. AcACT expressions showed no significant differences (p > 0.01) in two cultivars L-SH and L-SY over three growth periods and under suitable conditions, low temperature, and short-day conditions. In addition, AcACT was used as an internal reference gene to analyse the expression of the alliinase gene (AcALL). AcALL expression trends in the roots, stems and leaves were consistent with those of diallyl disulphide and diallyl trisulphide. Thus, AcACT is highly conserved and can be used as a suitable internal reference gene when analysing gene expression in tillering onion.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43130660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62323
Frank Bruno Vieira de Sousa, Á. Cruz, Daniela Francisca Soares, C. Cunha, J. Holzbach
Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.
{"title":"Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves","authors":"Frank Bruno Vieira de Sousa, Á. Cruz, Daniela Francisca Soares, C. Cunha, J. Holzbach","doi":"10.14393/bj-v39n0a2023-62323","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62323","url":null,"abstract":"Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46336379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62426
Aliya Riaz, Sana Ahmad, Ayesha Siddiqui, F. Jabeen, Farah Tariq, Shah Ali Ul Qader
1,4-alpha-D-glucan glucanohydrolase is among the most widely used commercial hydrolytic enzymes acting randomly on the glycosidic linkages of starch resulting in its saccharification and liquefaction. Its applicability in different industries can be improved by enhancing its stability and reusability. Therefore, in the present study attempts have been made to enhance the industrial applicability of 1,4-alpha-D-glucan glucanohydrolase from Bacillus subtilis KIBGE-HAR by adapting immobilization technology. The study developed mechanically stable, enzyme containing gel-frameworks using two support matrices including agar-agar, a natural polysaccharide and polyacrylamide gel, a synthetic organic polymer. These catalytic gel-scaffolds were compared with each other in terms of kinetics and stability of entrapped 1,4-α-D-glucan glucanohydrolase. In case of polyacrylamide gel, Km value for immobilized enzyme increased to 7.95 mg/mL, while immobilization in agar-agar resulted in decreased Km value i.e 0.277 mg/mL as compared to free enzyme. It was found that immobilized enzyme showed maximum activity at 70 °C in both the supports as compared to free enzyme having maximum activity at 60 °C. Immobilized 1,4-α-D-glucan glucanohydrolase exhibited no change in optimal pH 7.0 before and after entrapment in polyacrylamide gel and agar-agar. The enzyme containing gel-scaffold was found suitable for repeated batches of starch liquefaction in industrial processes. Agar-agar entrapped 1,4-α-D-glucanglucanohydrolase was capable to degrade starch up to seven repeated operational cycles whereas polyacrylamide entrapped enzyme conserved its activity up to sixth operational cycle.
1,4- α - d -葡聚糖葡聚糖水解酶是应用最广泛的商业水解酶之一，它随机作用于淀粉的糖苷键，导致淀粉的糖化和液化。通过增强其稳定性和可重用性，可以提高其在不同行业的适用性。因此，本研究尝试采用固定化技术提高枯草芽孢杆菌KIBGE-HAR中1,4- α - d -葡聚糖葡聚糖水解酶的工业适用性。该研究开发了机械稳定、含有酶的凝胶框架，使用两种支撑基质，包括琼脂(一种天然多糖)和聚丙烯酰胺凝胶(一种合成有机聚合物)。对这些催化凝胶支架包埋的1,4-α- d -葡聚糖葡聚糖水解酶的动力学和稳定性进行了比较。在聚丙烯酰胺凝胶中，固定化酶的Km值增加到7.95 mg/mL，而琼脂中固定化酶的Km值比游离酶降低了0.277 mg/mL。结果表明，固定化酶在70℃时活性最高，而游离酶在60℃时活性最高。固定化的1,4-α- d -葡聚糖葡聚糖水解酶在聚丙烯酰胺凝胶和琼脂包埋前后的最佳pH 7.0没有变化。发现含酶凝胶支架适用于工业过程中淀粉的重复批次液化。琼脂包埋的1,4-α- d -葡聚糖水解酶能够降解淀粉长达7个重复的操作循环，而聚丙烯酰胺包埋的酶在第6个操作循环中保持其活性。
{"title":"Fabrication of 1,4-alpha-D-glucan glucanohydrolase holding Gel-Scaffolds using Agar-Agar, a natural polysaccharide and Polyacrylamide, a synthetic organic polymer for continuous liquefaction of starch","authors":"Aliya Riaz, Sana Ahmad, Ayesha Siddiqui, F. Jabeen, Farah Tariq, Shah Ali Ul Qader","doi":"10.14393/bj-v39n0a2023-62426","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62426","url":null,"abstract":"1,4-alpha-D-glucan glucanohydrolase is among the most widely used commercial hydrolytic enzymes acting randomly on the glycosidic linkages of starch resulting in its saccharification and liquefaction. Its applicability in different industries can be improved by enhancing its stability and reusability. Therefore, in the present study attempts have been made to enhance the industrial applicability of 1,4-alpha-D-glucan glucanohydrolase from Bacillus subtilis KIBGE-HAR by adapting immobilization technology. The study developed mechanically stable, enzyme containing gel-frameworks using two support matrices including agar-agar, a natural polysaccharide and polyacrylamide gel, a synthetic organic polymer. These catalytic gel-scaffolds were compared with each other in terms of kinetics and stability of entrapped 1,4-α-D-glucan glucanohydrolase. In case of polyacrylamide gel, Km value for immobilized enzyme increased to 7.95 mg/mL, while immobilization in agar-agar resulted in decreased Km value i.e 0.277 mg/mL as compared to free enzyme. It was found that immobilized enzyme showed maximum activity at 70 °C in both the supports as compared to free enzyme having maximum activity at 60 °C. Immobilized 1,4-α-D-glucan glucanohydrolase exhibited no change in optimal pH 7.0 before and after entrapment in polyacrylamide gel and agar-agar. The enzyme containing gel-scaffold was found suitable for repeated batches of starch liquefaction in industrial processes. Agar-agar entrapped 1,4-α-D-glucanglucanohydrolase was capable to degrade starch up to seven repeated operational cycles whereas polyacrylamide entrapped enzyme conserved its activity up to sixth operational cycle.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43065158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62601
Osléias Ferreira Aguiar, João David Batista Lisboa, Ruy Bessa Lopes, Lucinewton Silva de Moura, Maxwell Barbosa de Santana
Animal welfare regards the quality of life and the environment in which animals live or are exposed. Hence the importance of studies assessing the environmental influence on the biology and behavior of fishes from the Colossoma macropomum species, considering their higher potential for fish-farming and as a test organism in scientific research. This study aimed to assess, in a controlled environment, the behavioral changes expressed by juveniles from the Colossoma macropomum species exposed to different ambient colors or social situations. The results did not show an influence from the different ambient colors or social situations on fish distribution in the water column. The color pattern showed dark tones in black- and blue-colored environments, and light tones in white-colored environments or with the presence of a mirror. The time of locomotor activity/frequency did not change in any of the treatments of exposure to environments with colors and a mirror. Moreover, the fishes remained with folded fins and a straight posture, maintaining a pattern of rhythmic operculum beating in a normal frequency range described for the species. This suggests that animal welfare did not change in any of the situations tested and that there was a pattern of adaptive response to the type of environment.
{"title":"Behavioral assessment of Tambaqui juveniles (Colossoma macropomum) exposed to different ambient colors and social stress","authors":"Osléias Ferreira Aguiar, João David Batista Lisboa, Ruy Bessa Lopes, Lucinewton Silva de Moura, Maxwell Barbosa de Santana","doi":"10.14393/bj-v39n0a2023-62601","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62601","url":null,"abstract":"Animal welfare regards the quality of life and the environment in which animals live or are exposed. Hence the importance of studies assessing the environmental influence on the biology and behavior of fishes from the Colossoma macropomum species, considering their higher potential for fish-farming and as a test organism in scientific research. This study aimed to assess, in a controlled environment, the behavioral changes expressed by juveniles from the Colossoma macropomum species exposed to different ambient colors or social situations. The results did not show an influence from the different ambient colors or social situations on fish distribution in the water column. The color pattern showed dark tones in black- and blue-colored environments, and light tones in white-colored environments or with the presence of a mirror. The time of locomotor activity/frequency did not change in any of the treatments of exposure to environments with colors and a mirror. Moreover, the fishes remained with folded fins and a straight posture, maintaining a pattern of rhythmic operculum beating in a normal frequency range described for the species. This suggests that animal welfare did not change in any of the situations tested and that there was a pattern of adaptive response to the type of environment.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42499433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62708
Lucio Marques Vieira-Souza, Felipe J Aidar, Jymmys Lopes dos Santos, E. Kalinine, Jhennyfer Aline Lima Rodrigues, José Uilien de Oliveira, Jéssica Denielle Matos dos Santos, Márcio Getirana Mota, Waleska dos Santos, Anderson Carlos Marçal
The present study aimed to investigate the effectiveness of high-intensity interval training (HIIT) in the body composition of Wistar rats. The HIIT protocol consisted of high-intensity swimming three times a week for four weeks. There were no differences between groups as to the Lee index. However, the weights of the perigonadal (p=0.001) and retroperitoneal (p=0.026) fats were significantly different between the Control Group (CG, n=10) vs. Trained Group (TG, n=10), respectively. There was also a significant increase in the body weight of the animals in TG (16.43%) and CG (7.19%) at the end of the experiment. These findings suggested that HIIT was not sufficient to improve significantly the body composition of rats.
{"title":"Changes in body composition of Wistar rats: effects of high-intensity interval training","authors":"Lucio Marques Vieira-Souza, Felipe J Aidar, Jymmys Lopes dos Santos, E. Kalinine, Jhennyfer Aline Lima Rodrigues, José Uilien de Oliveira, Jéssica Denielle Matos dos Santos, Márcio Getirana Mota, Waleska dos Santos, Anderson Carlos Marçal","doi":"10.14393/bj-v39n0a2023-62708","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62708","url":null,"abstract":"The present study aimed to investigate the effectiveness of high-intensity interval training (HIIT) in the body composition of Wistar rats. The HIIT protocol consisted of high-intensity swimming three times a week for four weeks. There were no differences between groups as to the Lee index. However, the weights of the perigonadal (p=0.001) and retroperitoneal (p=0.026) fats were significantly different between the Control Group (CG, n=10) vs. Trained Group (TG, n=10), respectively. There was also a significant increase in the body weight of the animals in TG (16.43%) and CG (7.19%) at the end of the experiment. These findings suggested that HIIT was not sufficient to improve significantly the body composition of rats.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41328036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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