{"title":"Age dependency of reduced scattering coefficient and viscoelastic character, and their relationship in Japanese female skin","authors":"Kenichiro Yoshida, Hiroki Yanagisawa","doi":"10.1364/boe.527858","DOIUrl":"https://doi.org/10.1364/boe.527858","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141652401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Somayeh Hashamdar, P. Parvin, Fatemeh Ramezani, Fatemeh Ahmadinouri, Amir Jafargholi, M. Refahizadeh, Mahzad Akbarpour, Mohammadreza Aghaei, O. Heidari
The lack of regeneration of injured neurons in the central and peripheral neural system leads to the failure of damaged tissue repair in patients. While there is no definitive cure for most neurodegenerative diseases, new therapeutic methods that cause the proliferation and differentiation of neurons are of interest. Challenges such as the inability of neuronal cells to proliferate after injury, the lack of a stimulus for initial stimulation, and the presence of the microenvironment around CNS neurons contain several inhibitory factors that prevent neuron regeneration, thus, creating a structure similar to the extracellular matrix helps the cell proliferation in current treatment. A rapid method of neuron-like cell differentiation of PC12 cells is introduced here based on a novel synthetic scaffold. Initially, poly allyldiglycol carbonate (CR-39) substrate is textured under a high dose of ArF UV excimer laser (1000 shot, 300 mJ/pulse equivalent to 300 J/cm2 at 193 nm) to create superficial periodic parallel microchannels with the micrometer spacing and sub-micron width. Ultraviolet treated CR-39 (UT CR-39) provides a suitable scaffold to speed up the transformation/differentiation of PC12 cells. The latter is pheochromocytoma of the rat adrenal medulla as an embryonic origin from the neural crest usually exposed to the nerve growth factor (NGF). In fact, PC12 cells are seeded on the microchannels and simultaneously are stimulated by coherent red photons at 660 nm within the therapeutic window. The UT CR-39 scaffold undergoes extra improvement of ∼ 30% after 12 minutes of laser activation regarding the photo-biomodulation (PBM) mechanism. The cell activation due to the coherent photons also gives rise to enhanced proliferation/differentiation. Here, PC12 cells are efficiently differentiated into neurons according to immunocytochemistry (ICC) and Western Blot verification tests based on MAP2 and synapsin-1 protein expression. In general, UT CR-39 acts as a superior bed to elevate the population of neuron-like cells up to threefold against those of untreated (control)ones. We conclude that the surface cross-linking due to UV exposure and subsequent induced hydrophilicity notably contribute to the neuron-like cell differentiation of PC12 without adding NGF.
{"title":"PC12 differentiation to neuron cells activated by a low-level laser at 660 nm on UV pre-treated CR-39 scaffolds with parallel microchannels","authors":"Somayeh Hashamdar, P. Parvin, Fatemeh Ramezani, Fatemeh Ahmadinouri, Amir Jafargholi, M. Refahizadeh, Mahzad Akbarpour, Mohammadreza Aghaei, O. Heidari","doi":"10.1364/boe.530876","DOIUrl":"https://doi.org/10.1364/boe.530876","url":null,"abstract":"The lack of regeneration of injured neurons in the central and peripheral neural system leads to the failure of damaged tissue repair in patients. While there is no definitive cure for most neurodegenerative diseases, new therapeutic methods that cause the proliferation and differentiation of neurons are of interest. Challenges such as the inability of neuronal cells to proliferate after injury, the lack of a stimulus for initial stimulation, and the presence of the microenvironment around CNS neurons contain several inhibitory factors that prevent neuron regeneration, thus, creating a structure similar to the extracellular matrix helps the cell proliferation in current treatment. A rapid method of neuron-like cell differentiation of PC12 cells is introduced here based on a novel synthetic scaffold. Initially, poly allyldiglycol carbonate (CR-39) substrate is textured under a high dose of ArF UV excimer laser (1000 shot, 300 mJ/pulse equivalent to 300 J/cm2 at 193 nm) to create superficial periodic parallel microchannels with the micrometer spacing and sub-micron width. Ultraviolet treated CR-39 (UT CR-39) provides a suitable scaffold to speed up the transformation/differentiation of PC12 cells. The latter is pheochromocytoma of the rat adrenal medulla as an embryonic origin from the neural crest usually exposed to the nerve growth factor (NGF). In fact, PC12 cells are seeded on the microchannels and simultaneously are stimulated by coherent red photons at 660 nm within the therapeutic window. The UT CR-39 scaffold undergoes extra improvement of ∼ 30% after 12 minutes of laser activation regarding the photo-biomodulation (PBM) mechanism. The cell activation due to the coherent photons also gives rise to enhanced proliferation/differentiation. Here, PC12 cells are efficiently differentiated into neurons according to immunocytochemistry (ICC) and Western Blot verification tests based on MAP2 and synapsin-1 protein expression. In general, UT CR-39 acts as a superior bed to elevate the population of neuron-like cells up to threefold against those of untreated (control)ones. We conclude that the surface cross-linking due to UV exposure and subsequent induced hydrophilicity notably contribute to the neuron-like cell differentiation of PC12 without adding NGF.","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141655015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Deep Learning Combined with Attention Mechanisms to Assist Radiologists in Enhancing Breast Cancer Diagnosis: A Study on Photoacoustic Imaging","authors":"Guoqiu Li, Zhibin Huang, Hongtian Tian, Huaiyu Wu, Jing Zheng, Mengyun Wang, Sijie Mo, Zhijie Chen, Jinfeng Xu, F. Dong","doi":"10.1364/boe.530249","DOIUrl":"https://doi.org/10.1364/boe.530249","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141662880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vamshi Damagatla, N. Boetti, L. Di Sieno, I. Bargigia, Fabio Negretti, Diego Pugliese, Davide Janner, Lorenzo Spinelli, A. Farina, A. Pifferi
{"title":"Use of bioresorbable fibers for short-wave infrared spectroscopy using time-domain diffuse optics","authors":"Vamshi Damagatla, N. Boetti, L. Di Sieno, I. Bargigia, Fabio Negretti, Diego Pugliese, Davide Janner, Lorenzo Spinelli, A. Farina, A. Pifferi","doi":"10.1364/boe.531681","DOIUrl":"https://doi.org/10.1364/boe.531681","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141665088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiang Wei, T. Hormel, L. Renner, Martha Neuringer, Yali Jia
{"title":"Wide-field OCT Angiography for Non-human Primate Retinal Imaging","authors":"Xiang Wei, T. Hormel, L. Renner, Martha Neuringer, Yali Jia","doi":"10.1364/boe.525839","DOIUrl":"https://doi.org/10.1364/boe.525839","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141669855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Timothy Quang, Golnar Mostashari, Elise M. Berning, Bindu Gopalan, Maria A. Lizarralde-Iragorri, Dianna Lovins, Arun Shet, Bruce J. Tromberg
{"title":"Non-invasive optical and laboratory hematologic biomarkers correlate in patients with sickle cell disease","authors":"Timothy Quang, Golnar Mostashari, Elise M. Berning, Bindu Gopalan, Maria A. Lizarralde-Iragorri, Dianna Lovins, Arun Shet, Bruce J. Tromberg","doi":"10.1364/boe.527770","DOIUrl":"https://doi.org/10.1364/boe.527770","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141677610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Priyanka Vasanthakumari, Renan Romano, Ramon Rosa, A. Salvio, Vladislav V. Yakovlev, Cristina Kurachi, Jason Hirshburg, Javier A Jo
{"title":"Pixel-level classification of pigmented skin cancer lesions using multispectral autofluorescence lifetime dermoscopy imaging","authors":"Priyanka Vasanthakumari, Renan Romano, Ramon Rosa, A. Salvio, Vladislav V. Yakovlev, Cristina Kurachi, Jason Hirshburg, Javier A Jo","doi":"10.1364/boe.523831","DOIUrl":"https://doi.org/10.1364/boe.523831","url":null,"abstract":"","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141684970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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