Xiaojing Xia, Greg Felsted, Anupum Pant, E. Dobretsova, P. Pauzauskie
Single-beam laser tweezers with a tunable NIR trapping wavelength are used to analyze cold Brownian motion (CBM) dynamics of ytterbium-doped alkali yttrium fluoride (Yb:MYF, M=Li, Na, K) nanostructures.
{"title":"Laser refrigeration of ytterbium-doped alkali-yttrium-fluoride nanostructures (Yb:MYF, M = K, Na, Li)","authors":"Xiaojing Xia, Greg Felsted, Anupum Pant, E. Dobretsova, P. Pauzauskie","doi":"10.1364/oma.2019.aw4e.2","DOIUrl":"https://doi.org/10.1364/oma.2019.aw4e.2","url":null,"abstract":"Single-beam laser tweezers with a tunable NIR trapping wavelength are used to analyze cold Brownian motion (CBM) dynamics of ytterbium-doped alkali yttrium fluoride (Yb:MYF, M=Li, Na, K) nanostructures.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88439948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kinesin molecules “walk” on microtubules with 8 nm steps. Typically, it is believed to not twist during the motilty. It is this very aspect that we probe using birefringent microspheres of liquid crystalline material RM257 (Merck) upon placing them under crossed polarizers during motility and also using the Optical Micro-Protractor that uses the optical tweezers and detect the twist and the torque generated by the kinesin molecules during stepping.
{"title":"Determination of twisting of kinesin molecules during stepping","authors":"Basudev Roy, Avin Ramaiya, E. Schäffer","doi":"10.1364/OMA.2019.AM2E.1","DOIUrl":"https://doi.org/10.1364/OMA.2019.AM2E.1","url":null,"abstract":"Kinesin molecules “walk” on microtubules with 8 nm steps. Typically, it is believed to not twist during the motilty. It is this very aspect that we probe using birefringent microspheres of liquid crystalline material RM257 (Merck) upon placing them under crossed polarizers during motility and also using the Optical Micro-Protractor that uses the optical tweezers and detect the twist and the torque generated by the kinesin molecules during stepping.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84102084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/brain.2019.bt3a.3
M. Harfouche, Timothy W. Dunn, Eva A Naumann, R. Horstmeyer
We present a micro-camera array microscope that images at cellular-level detail across hundreds of square centimeters. We demonstrate how this microscope can image the behavior and fluorescent neural activity of freely swimming zebrafish
{"title":"Imaging the behavior and neural activity of freely moving organisms with a gigapixel microscope","authors":"M. Harfouche, Timothy W. Dunn, Eva A Naumann, R. Horstmeyer","doi":"10.1364/brain.2019.bt3a.3","DOIUrl":"https://doi.org/10.1364/brain.2019.bt3a.3","url":null,"abstract":"We present a micro-camera array microscope that images at cellular-level detail across hundreds of square centimeters. We demonstrate how this microscope can image the behavior and fluorescent neural activity of freely swimming zebrafish","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74014259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/brain.2019.bt2a.2
Lisa Beckmann, Xian Zhang, Hao F. Zhang
A chronically implanted microprism in the rodent cortex enables cross-sectional imaging of all the key cortical layers. We determined the time course of recovery from this surgical implantation using visible-light optical coherence tomography angiography.
{"title":"Visible-light optical coherence tomography investigation into vasculature changes following microprism implantation","authors":"Lisa Beckmann, Xian Zhang, Hao F. Zhang","doi":"10.1364/brain.2019.bt2a.2","DOIUrl":"https://doi.org/10.1364/brain.2019.bt2a.2","url":null,"abstract":"A chronically implanted microprism in the rodent cortex enables cross-sectional imaging of all the key cortical layers. We determined the time course of recovery from this surgical implantation using visible-light optical coherence tomography angiography.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74889090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.jt4a.45
N. Iqbal, A. Anastasiou, C. Maddi, Mostafa El-Raif, P. Giannoudis, A. Jha
An IR femtosecond pulsed laser was used for micropatterning of biomineral containing chitosan membranes, aiming to enhance bone mineralization and angiogenesis. Pre and post irradiation materials have been characterized with XRD, SEM and spectroscopic techniques.
{"title":"Fabrication of Multi-Layered Bone Scaffolds using Femtosecond Pulsed Lasers","authors":"N. Iqbal, A. Anastasiou, C. Maddi, Mostafa El-Raif, P. Giannoudis, A. Jha","doi":"10.1364/boda.2019.jt4a.45","DOIUrl":"https://doi.org/10.1364/boda.2019.jt4a.45","url":null,"abstract":"An IR femtosecond pulsed laser was used for micropatterning of biomineral containing chitosan membranes, aiming to enhance bone mineralization and angiogenesis. Pre and post irradiation materials have been characterized with XRD, SEM and spectroscopic techniques.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73958536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Combined with optical clearing protocols, light-sheet microscopy offers rapid and sensitive imaging of whole organs. We report a light sheet microscope with isotropic, submicron resolution that is compatible a refractive index range of 1.33-1.56.
{"title":"A multimodal light-sheet microscope that is compatible with all clearing techniques","authors":"Tonmoy Chakraborty, K. Dean, Hu Zhao, R. Fiolka","doi":"10.1364/NTM.2019.NW5C.6","DOIUrl":"https://doi.org/10.1364/NTM.2019.NW5C.6","url":null,"abstract":"Combined with optical clearing protocols, light-sheet microscopy offers rapid and sensitive imaging of whole organs. We report a light sheet microscope with isotropic, submicron resolution that is compatible a refractive index range of 1.33-1.56.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74212913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diffuse in-vivo Flow Cytometry is a new method to enumerate fluorescently-labeled circulating tumor cells in-vivo. Herein, we investigated the use of a folate receptor-targeted fluorescence probe EC17 for labeling of target cells in the bloodstream.
{"title":"Labeling Circulating Tumor Cells with a Folate Receptor Targeted Probe for Diffuse in-vivo Flow Cytometry","authors":"Roshani A. Patil, S. Madduri, P. Low, M. Niedre","doi":"10.1364/omp.2019.om3d.4","DOIUrl":"https://doi.org/10.1364/omp.2019.om3d.4","url":null,"abstract":"Diffuse in-vivo Flow Cytometry is a new method to enumerate fluorescently-labeled circulating tumor cells in-vivo. Herein, we investigated the use of a folate receptor-targeted fluorescence probe EC17 for labeling of target cells in the bloodstream.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74229308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jason T. Smith, Nathan Un, Ruoyang Yao, Nattawut Sinsuebphon, Alena Rudkouskaya, J. Mazurkiewicz, Margarida M Barroso, Pingkun Yan, X. Intes
Herein, we present a novel workflow based on Deep Learning (DL) trained on synthetic data to quantify fluorescence lifetime imaging of experimental data across multiple microscopic and macroscopic applications with unprecedented accuracy and computational speed.
{"title":"Fluorescent Lifetime Imaging improved via Deep Learning","authors":"Jason T. Smith, Nathan Un, Ruoyang Yao, Nattawut Sinsuebphon, Alena Rudkouskaya, J. Mazurkiewicz, Margarida M Barroso, Pingkun Yan, X. Intes","doi":"10.1364/ntm.2019.nm3c.4","DOIUrl":"https://doi.org/10.1364/ntm.2019.nm3c.4","url":null,"abstract":"Herein, we present a novel workflow based on Deep Learning (DL) trained on synthetic data to quantify fluorescence lifetime imaging of experimental data across multiple microscopic and macroscopic applications with unprecedented accuracy and computational speed.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78435453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
diSPIM is used to render 3D digital histological images on large specimens. By comparing dual-view deconvolved results with the corresponding single-view images, we demonstrate that dual-view imaging can provide higher image accuracy.
{"title":"Dual-view Inverted Selective Plane Illumination Microscopy for Accurate 3D Digital Pathology on Large Specimens","authors":"Bihe Hu, Guang Li, Andrew B. Sholl, J. Q. Brown","doi":"10.1364/NTM.2019.NT3C.3","DOIUrl":"https://doi.org/10.1364/NTM.2019.NT3C.3","url":null,"abstract":"diSPIM is used to render 3D digital histological images on large specimens. By comparing dual-view deconvolved results with the corresponding single-view images, we demonstrate that dual-view imaging can provide higher image accuracy.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74941664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aaron Smith, A. Samiei, J. Lyne, C. Post, Shona Stewart, Heather Gomer, Jihang Wang, Jeffrey A. Cohen, P. Treado
Improved patient outcomes highlight the need to avoid errors in surgery. Molecular Chemical Imaging (MCI) aids in identifying critical structures without reagents and in vivo results are presented for a range of surgical applications.
{"title":"Molecular Chemical Imaging of Critical Anatomical Structures in vivo","authors":"Aaron Smith, A. Samiei, J. Lyne, C. Post, Shona Stewart, Heather Gomer, Jihang Wang, Jeffrey A. Cohen, P. Treado","doi":"10.1364/omp.2019.om4d.3","DOIUrl":"https://doi.org/10.1364/omp.2019.om4d.3","url":null,"abstract":"Improved patient outcomes highlight the need to avoid errors in surgery. Molecular Chemical Imaging (MCI) aids in identifying critical structures without reagents and in vivo results are presented for a range of surgical applications.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78166451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：