Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.JW4C.2
Alex Matlock, Ji Yi, L. Tian
We present multiplexed Intensity Diffraction Tomography (mIDT) for live biological sample imaging. We achieve 100X imaging speed improvements using model-based multiplexed LED illuminations that maintain high-quality 3D object reconstructions.
{"title":"Multiplexed Intensity Diffraction Tomography (mIDT) for Dynamic, Label-Free Volumetric Biological Imaging","authors":"Alex Matlock, Ji Yi, L. Tian","doi":"10.1364/BODA.2019.JW4C.2","DOIUrl":"https://doi.org/10.1364/BODA.2019.JW4C.2","url":null,"abstract":"We present multiplexed Intensity Diffraction Tomography (mIDT) for live biological sample imaging. We achieve 100X imaging speed improvements using model-based multiplexed LED illuminations that maintain high-quality 3D object reconstructions.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79335458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We report a method for epi-fluorescent light-sheet microscopy with a single-element detector. This method is based on spatial frequency projection imaging and utilizes PSF engineering to enhance the depth of field.
{"title":"Toward Single-Lens Epi-Fluorescent Light Sheet Microscopy with Single-Pixel Detection","authors":"J. Field, R. Bartels","doi":"10.1364/NTM.2019.NW5C.3","DOIUrl":"https://doi.org/10.1364/NTM.2019.NW5C.3","url":null,"abstract":"We report a method for epi-fluorescent light-sheet microscopy with a single-element detector. This method is based on spatial frequency projection imaging and utilizes PSF engineering to enhance the depth of field.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76726276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Hellwig, M. Brinkmann, Alexander Fast, C. Evans, C. Fallnich
We present high-speed multicolor coherent Raman imaging enabled by a robust fiber optical parametric oscillator tunable in 5 ms per arbitrary wavelength step without the need for a mechanical delay.
{"title":"High-speed Multicolor Coherent Raman Imaging Enabled by a Novel Fiber Optical Parametric Oscillator","authors":"T. Hellwig, M. Brinkmann, Alexander Fast, C. Evans, C. Fallnich","doi":"10.1364/NTM.2019.NT1C.2","DOIUrl":"https://doi.org/10.1364/NTM.2019.NT1C.2","url":null,"abstract":"We present high-speed multicolor coherent Raman imaging enabled by a robust fiber optical parametric oscillator tunable in 5 ms per arbitrary wavelength step without the need for a mechanical delay.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78512353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.jw4c.5
Yayao Ma, Riwei Jin, Liang Gao
We present a GPU-accelerated constrained reconstruction algorithm which improves image quality and reconstruction speed for compressed fluorescence lifetime microscopy.
提出了一种gpu加速约束重建算法，提高了压缩荧光寿命显微镜的图像质量和重建速度。
{"title":"Development of a GPU-accelerated constrained reconstruction algorithm for compressed fluorescence lifetime imaging microscopy","authors":"Yayao Ma, Riwei Jin, Liang Gao","doi":"10.1364/boda.2019.jw4c.5","DOIUrl":"https://doi.org/10.1364/boda.2019.jw4c.5","url":null,"abstract":"We present a GPU-accelerated constrained reconstruction algorithm which improves image quality and reconstruction speed for compressed fluorescence lifetime microscopy.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80440962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.JW3B.3
D. Faccio, A. Lyons, F. Tonolini, R. Murray-Smith
Recent progress in computational techniques and quantum detection technologies allow to reconstruct images of objects with mm-resolution hidden inside deep (5 cm), strongly diffusive, opaque materials with applications in deep body imaging.
{"title":"Deep-imaging with time-of-flight diffusive optical tomography","authors":"D. Faccio, A. Lyons, F. Tonolini, R. Murray-Smith","doi":"10.1364/BODA.2019.JW3B.3","DOIUrl":"https://doi.org/10.1364/BODA.2019.JW3B.3","url":null,"abstract":"Recent progress in computational techniques and quantum detection technologies allow to reconstruct images of objects with mm-resolution hidden inside deep (5 cm), strongly diffusive, opaque materials with applications in deep body imaging.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86197581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.JT4A.35
Juho Ala-Myllymäki, T. Paakkonen, J. Töyräs, I. Afara
We investigate the potential of near-infrared spectroscopy for estimating the properties of human meniscus during arthroscopy. In vitro predictive models were developed and tested on ex vivo arthroscopic near-infrared spectroscopy measurements.
{"title":"Arthroscopic Near-Infrared Spectroscopic Prediction of Human Meniscus Properties","authors":"Juho Ala-Myllymäki, T. Paakkonen, J. Töyräs, I. Afara","doi":"10.1364/BODA.2019.JT4A.35","DOIUrl":"https://doi.org/10.1364/BODA.2019.JT4A.35","url":null,"abstract":"We investigate the potential of near-infrared spectroscopy for estimating the properties of human meniscus during arthroscopy. In vitro predictive models were developed and tested on ex vivo arthroscopic near-infrared spectroscopy measurements.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78572246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I will present two chemical imaging strategies to address two existing fundamental challenges in optical bio-imaging. First, we devised a Bioorthogonal Chemical Imaging platform suited for probing the dynamics of small bio-molecules. Second, we developed a super-multiplex vibrational imaging method, coupling the narrow Raman linewidth with sensitivity boost from resonance enhancement. It is capable of resolving up to 24 colors simultaneously.
{"title":"Chemical imaging for Biomedicine","authors":"Lu Wei","doi":"10.1364/OMP.2019.OT3D.3","DOIUrl":"https://doi.org/10.1364/OMP.2019.OT3D.3","url":null,"abstract":"I will present two chemical imaging strategies to address two existing fundamental challenges in optical bio-imaging. First, we devised a Bioorthogonal Chemical Imaging platform suited for probing the dynamics of small bio-molecules. Second, we developed a super-multiplex vibrational imaging method, coupling the narrow Raman linewidth with sensitivity boost from resonance enhancement. It is capable of resolving up to 24 colors simultaneously.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73844180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.JT4A.5
J. Bhardwaj, A. Nayak
The proposed method has proved an efficient methodology in the transform based image fusion schemes .The attractable properties of both Lifting wavelet and KL Transform (LWKL method) are employed.
该方法利用了提升小波变换和KL变换(LWKL方法)的优点，是一种有效的图像融合方法。
{"title":"Lifting Wavelet and KL Transform(LWKL) Based CT and MRI Image Fusion Scheme","authors":"J. Bhardwaj, A. Nayak","doi":"10.1364/BODA.2019.JT4A.5","DOIUrl":"https://doi.org/10.1364/BODA.2019.JT4A.5","url":null,"abstract":"The proposed method has proved an efficient methodology in the transform based image fusion schemes .The attractable properties of both Lifting wavelet and KL Transform (LWKL method) are employed.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80639139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.jt4a.12
L. Schmidl, Luise Röpke, M. Ceanga, J. Kreye, N. K. Wenke, H. Haselmann, H. Prüss, C. Geis
We investigated the pathogenic role of anti-N-Methyl-D-aspartate receptor (NMDAR) antibodies in neurons and in a mouse model of autoimmune encephalitis using LSM and super-resolution microscopy. We found a functional reduction of synaptic NMDARs.
我们利用LSM和超分辨率显微镜研究了抗n -甲基- d -天冬氨酸受体(NMDAR)抗体在神经元和自身免疫性脑炎小鼠模型中的致病作用。我们发现突触NMDARs的功能性减少。
{"title":"Human anti-NR1 autoantibodies induce synaptic pathology with functional relevant loss of postsynaptic NMDA receptors","authors":"L. Schmidl, Luise Röpke, M. Ceanga, J. Kreye, N. K. Wenke, H. Haselmann, H. Prüss, C. Geis","doi":"10.1364/boda.2019.jt4a.12","DOIUrl":"https://doi.org/10.1364/boda.2019.jt4a.12","url":null,"abstract":"We investigated the pathogenic role of anti-N-Methyl-D-aspartate receptor (NMDAR) antibodies in neurons and in a mouse model of autoimmune encephalitis using LSM and super-resolution microscopy. We found a functional reduction of synaptic NMDARs.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82623527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Max H Kushner, Juan Wang, A. Ozer, J. Ray, Hening Lin, J. Lis, W. Zipfel
Femto-Seq is a new nonlinear optical methodology that provides a means to determine the chromatin environment at the base-pair level at and around any site in the nucleus that can be identified by imaging.
{"title":"Femto-Seq: A New Nonlinear Microcopy Approach for Elucidating Chromatin Structure at the Single Gene Level","authors":"Max H Kushner, Juan Wang, A. Ozer, J. Ray, Hening Lin, J. Lis, W. Zipfel","doi":"10.1364/ntm.2019.ns2b.5","DOIUrl":"https://doi.org/10.1364/ntm.2019.ns2b.5","url":null,"abstract":"Femto-Seq is a new nonlinear optical methodology that provides a means to determine the chromatin environment at the base-pair level at and around any site in the nucleus that can be identified by imaging.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83210027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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