Pub Date : 2019-04-14DOI: 10.1364/boda.2019.jt4a.21
Jeonghwan Son, B. Mandracchia, Michael D. Caponegro, Styliani-Anna E. Tsirka, Shu Jia
The miniaturized one-photon epi-fluorescence microscopy (miniscopy) has emerged as a powerful tool for in vivo functional brain imaging. Here, we report a computational method to improve the image quality of miniscopy.
{"title":"The gradient and frequency-wise analysis improves wide-field imaging in miniaturized one-photon microscopy","authors":"Jeonghwan Son, B. Mandracchia, Michael D. Caponegro, Styliani-Anna E. Tsirka, Shu Jia","doi":"10.1364/boda.2019.jt4a.21","DOIUrl":"https://doi.org/10.1364/boda.2019.jt4a.21","url":null,"abstract":"The miniaturized one-photon epi-fluorescence microscopy (miniscopy) has emerged as a powerful tool for in vivo functional brain imaging. Here, we report a computational method to improve the image quality of miniscopy.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90845037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.DW2B.4
H. C. Wood, K. Harrington, J. Knight, T. Birks, J. Stone
We describe an imaging fiber bundle that uses 11,000 doped silica cores in an air-filled cladding to image features as small as 3 µm at 1 µm wavelength.
{"title":"High-resolution air-clad imaging fibers","authors":"H. C. Wood, K. Harrington, J. Knight, T. Birks, J. Stone","doi":"10.1364/BODA.2019.DW2B.4","DOIUrl":"https://doi.org/10.1364/BODA.2019.DW2B.4","url":null,"abstract":"We describe an imaging fiber bundle that uses 11,000 doped silica cores in an air-filled cladding to image features as small as 3 µm at 1 µm wavelength.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89292617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.jt4a.52
Jong Moon Lee, Ibrahim Misbah, Wei-Chuan Shih
Plasmonic arrays fabricated by low-cost nanosphere lithography feature disorderliness and corresponding non-uniform index sensitivity. A calibration technique based on hyperspectral imaging has been implemented to regularize the sensitivity.
{"title":"Regularizing refractive index sensitivity for disordered plasmonic array","authors":"Jong Moon Lee, Ibrahim Misbah, Wei-Chuan Shih","doi":"10.1364/boda.2019.jt4a.52","DOIUrl":"https://doi.org/10.1364/boda.2019.jt4a.52","url":null,"abstract":"Plasmonic arrays fabricated by low-cost nanosphere lithography feature disorderliness and corresponding non-uniform index sensitivity. A calibration technique based on hyperspectral imaging has been implemented to regularize the sensitivity.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85840589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Using high throughput wide field SH imaging we probe the orientational ordering of water in freestanding lipid membrane hydration shells, and determine real-time membrane potential maps (400 nm, ~100 ms). Applications to neurons are investigated.
{"title":"Label-free dynamic lipid membrane potential imaging","authors":"O. B. Tarun, S. Roke","doi":"10.1364/NTM.2019.NW1C.1","DOIUrl":"https://doi.org/10.1364/NTM.2019.NW1C.1","url":null,"abstract":"Using high throughput wide field SH imaging we probe the orientational ordering of water in freestanding lipid membrane hydration shells, and determine real-time membrane potential maps (400 nm, ~100 ms). Applications to neurons are investigated.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89387663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BRAIN.2019.BM3A.5
Nicolas C. Pégard, L. Waller, H. Adesnik
We synthesize custom light fields by simultaneously modulating light in the spatial and angular domain. Experimental results show new applications for high-resolution 3D displays and for high-speed sculpted illumination with enhanced depth selectivity.
{"title":"Holographic Display and Volumetric Light Sculpting by Dynamic Synthesis of 4d Light Fields","authors":"Nicolas C. Pégard, L. Waller, H. Adesnik","doi":"10.1364/BRAIN.2019.BM3A.5","DOIUrl":"https://doi.org/10.1364/BRAIN.2019.BM3A.5","url":null,"abstract":"We synthesize custom light fields by simultaneously modulating light in the spatial and angular domain. Experimental results show new applications for high-resolution 3D displays and for high-speed sculpted illumination with enhanced depth selectivity.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77050832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.dt3b.7
Alasdair H. Fikouras, M. Schubert, Markus Karl, J. D. Kumar, S. Powis, A. Falco, M. Gather
We report the application of semiconductor nanodisk lasers within living cells. Our lasers have volumes 1000-fold smaller than eukaryotic nuclei, ultralow pulse energy lasing thresholds (Eth≈0.13pJ), and provide excellent spectral stability.
{"title":"Intracellular Semiconductor Nanodisk Lasers","authors":"Alasdair H. Fikouras, M. Schubert, Markus Karl, J. D. Kumar, S. Powis, A. Falco, M. Gather","doi":"10.1364/boda.2019.dt3b.7","DOIUrl":"https://doi.org/10.1364/boda.2019.dt3b.7","url":null,"abstract":"We report the application of semiconductor nanodisk lasers within living cells. Our lasers have volumes 1000-fold smaller than eukaryotic nuclei, ultralow pulse energy lasing thresholds (Eth≈0.13pJ), and provide excellent spectral stability.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"149 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91125570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.DM3B.3
Hao F. Zhang, Brian T. Soetikno, Lisa Beckmann, Xian Zhang, Ian Rubinoff, R. Kuranov
We developed a combined cross-correlation and graph-search segmentation techniques, to reliably extract the backscattered light spectrum so that we can improve the accuracy of retinal oximetry using visible-light OCT.
{"title":"Improving accuracy of visible-light OCT oximetry in rodents and humans","authors":"Hao F. Zhang, Brian T. Soetikno, Lisa Beckmann, Xian Zhang, Ian Rubinoff, R. Kuranov","doi":"10.1364/BODA.2019.DM3B.3","DOIUrl":"https://doi.org/10.1364/BODA.2019.DM3B.3","url":null,"abstract":"We developed a combined cross-correlation and graph-search segmentation techniques, to reliably extract the backscattered light spectrum so that we can improve the accuracy of retinal oximetry using visible-light OCT.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74090601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.JT4A.22
Julie Bouhy, Angel Martin Fernandez Alvarez, Catherine Charles, O. Deparis
We adapted a polarizing microscopy technique involving image processing in order to determine birefringence patterns in anisotropic biological tissues. The methodology is applied to the study of parchments’ material degradation and internal strains.
{"title":"Adapted polarizing microscopy technique for the determination of birefringence patterns in parchments","authors":"Julie Bouhy, Angel Martin Fernandez Alvarez, Catherine Charles, O. Deparis","doi":"10.1364/BODA.2019.JT4A.22","DOIUrl":"https://doi.org/10.1364/BODA.2019.JT4A.22","url":null,"abstract":"We adapted a polarizing microscopy technique involving image processing in order to determine birefringence patterns in anisotropic biological tissues. The methodology is applied to the study of parchments’ material degradation and internal strains.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"310 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77634131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.dt2b.3
P. Stremplewski, E. Auksorius, P. Wnuk, Łukasz Kozoń, P. Garstecki, M. Wojtkowski
We report on a system that is able to significantly reduce cross-talk and speckle noise in Fourier domain full-field optical coherence tomography. It is achieved by fast phase modulation of a laser wavefront and angular compounding.
{"title":"Speckle-free and cross-talk-free imaging in Fourier domain full-field optical coherence tomography","authors":"P. Stremplewski, E. Auksorius, P. Wnuk, Łukasz Kozoń, P. Garstecki, M. Wojtkowski","doi":"10.1364/boda.2019.dt2b.3","DOIUrl":"https://doi.org/10.1364/boda.2019.dt2b.3","url":null,"abstract":"We report on a system that is able to significantly reduce cross-talk and speckle noise in Fourier domain full-field optical coherence tomography. It is achieved by fast phase modulation of a laser wavefront and angular compounding.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82462121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.jt4a.50
J. Bogusławski, Natalia Pacocha, Michał Horka, M. Wojtkowski, P. Garstecki
A microfluidic platform for an optical, label-free screening of bacteria growth in nanoliter droplets is demonstrated. We show that based on droplet’s scattering properties we can perform a reliable binary readout.
{"title":"Optofluidic Platform for Bacteria Screening in Nanoliter Droplets","authors":"J. Bogusławski, Natalia Pacocha, Michał Horka, M. Wojtkowski, P. Garstecki","doi":"10.1364/boda.2019.jt4a.50","DOIUrl":"https://doi.org/10.1364/boda.2019.jt4a.50","url":null,"abstract":"A microfluidic platform for an optical, label-free screening of bacteria growth in nanoliter droplets is demonstrated. We show that based on droplet’s scattering properties we can perform a reliable binary readout.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81578956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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