Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.DW2B.3
Ibrahim Misbah, Wei-Chuan Shih
Plasmonic hybridization in a gold-silver alloy nanodisks array results in a pair of high and low energy LSPR modes. This high energy mode is applied for colorimetric detection of sub-nM and sub-monolayer biotin-streptavidin surface binding using a smartphone camera.
{"title":"Gold-Silver Alloy Nanodisk Array for Smartphone Colorimetric Biosensing","authors":"Ibrahim Misbah, Wei-Chuan Shih","doi":"10.1364/BODA.2019.DW2B.3","DOIUrl":"https://doi.org/10.1364/BODA.2019.DW2B.3","url":null,"abstract":"Plasmonic hybridization in a gold-silver alloy nanodisks array results in a pair of high and low energy LSPR modes. This high energy mode is applied for colorimetric detection of sub-nM and sub-monolayer biotin-streptavidin surface binding using a smartphone camera.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"7 17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82945146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.DM2B.5
Jürgen W. Czarske, E. Scharf, R. Kuschmierz
We present a holographic endoscope with tiny footprint. A novel non-invasive in situ calibration of a coherent fiber bundle in combination with a galvanometer scanner enables fast, minimally invasive 3D measurements.
{"title":"Ultrathin lensless fiber endoscope with in situ calibration for 3D imaging","authors":"Jürgen W. Czarske, E. Scharf, R. Kuschmierz","doi":"10.1364/BODA.2019.DM2B.5","DOIUrl":"https://doi.org/10.1364/BODA.2019.DM2B.5","url":null,"abstract":"We present a holographic endoscope with tiny footprint. A novel non-invasive in situ calibration of a coherent fiber bundle in combination with a galvanometer scanner enables fast, minimally invasive 3D measurements.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83527040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Schie, J. Rüger, S. Mondol, Anuradha Ramoji, U. Neugebauer, C. Krafft, J. Popp
We present a HTS-RS platform for a rapid and label-free macromolecular fingerprinting of tens of thousands eukaryotic cells. The proposed platform combines automated imaging microscopy with Raman spectroscopy to enable a rapid label-free cell screening.
{"title":"High-throughput screening Raman spectroscopy (HTS-RS) platform for label-free single cell analysis","authors":"I. Schie, J. Rüger, S. Mondol, Anuradha Ramoji, U. Neugebauer, C. Krafft, J. Popp","doi":"10.1364/OMP.2019.OT2D.3","DOIUrl":"https://doi.org/10.1364/OMP.2019.OT2D.3","url":null,"abstract":"We present a HTS-RS platform for a rapid and label-free macromolecular fingerprinting of tens of thousands eukaryotic cells. The proposed platform combines automated imaging microscopy with Raman spectroscopy to enable a rapid label-free cell screening.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89449323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.dt1b.3
T. Sawyer, Jennifer W. Koevary, P. Rice, J. Barton
Ovarian cancer is the deadliest gynecologic cancer, but can be addressed with early detection. We investigate fluorescence and multiphoton imaging for imaging ovarian cancer, finding that tissue changes can be detected through quantitative analysis.
{"title":"Fluorescence and Multiphoton Imaging of a Mouse Model of Spontaneous Ovarian Cancer","authors":"T. Sawyer, Jennifer W. Koevary, P. Rice, J. Barton","doi":"10.1364/boda.2019.dt1b.3","DOIUrl":"https://doi.org/10.1364/boda.2019.dt1b.3","url":null,"abstract":"Ovarian cancer is the deadliest gynecologic cancer, but can be addressed with early detection. We investigate fluorescence and multiphoton imaging for imaging ovarian cancer, finding that tissue changes can be detected through quantitative analysis.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79565827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.DW2B.1
Phuong-Diem Nguyen, Adley Gin, Judith Su
Novel lipid coated microtoroid optical resonators was developed for membrane interaction. The proposed platform significantly enhanced the detection of cholera toxin in term of time and detection limit compared to fluorescent-based assay.
{"title":"Detection of membrane binding events using microtoroid optical resonators","authors":"Phuong-Diem Nguyen, Adley Gin, Judith Su","doi":"10.1364/BODA.2019.DW2B.1","DOIUrl":"https://doi.org/10.1364/BODA.2019.DW2B.1","url":null,"abstract":"Novel lipid coated microtoroid optical resonators was developed for membrane interaction. The proposed platform significantly enhanced the detection of cholera toxin in term of time and detection limit compared to fluorescent-based assay.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79966403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/boda.2019.ds1a.3
R. Shaikh, E. Nippolainen, V. Virtanen, L. Rieppo, J. Haas, B. Mizaikoff, V. Artyushenko, O. Bibikova, I. Afara, S. Saarakkala, J. Töyräs
We introduce classification models based on partial least squares discriminant-analysis (PLS-DA) for estimating cartilage integrity (assessed by OARSI grade) based on mid-infrared spectra of cartilage matrix. The best model achieved accuracy of 84%.
{"title":"Mid-infrared Spectroscopic Assessment of Cartilage Degeneration","authors":"R. Shaikh, E. Nippolainen, V. Virtanen, L. Rieppo, J. Haas, B. Mizaikoff, V. Artyushenko, O. Bibikova, I. Afara, S. Saarakkala, J. Töyräs","doi":"10.1364/boda.2019.ds1a.3","DOIUrl":"https://doi.org/10.1364/boda.2019.ds1a.3","url":null,"abstract":"We introduce classification models based on partial least squares discriminant-analysis (PLS-DA) for estimating cartilage integrity (assessed by OARSI grade) based on mid-infrared spectra of cartilage matrix. The best model achieved accuracy of 84%.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78078053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deep learning based tomographic phase microscopy with blind structured illumination (DeepTomo) is a unique imaging mechanism that combines simplified optical design with deep neural networks. The simulation results show that DeepTomo has high spatiotemporal resolution.
{"title":"Deep learning based tomographic phase microscopy with blind structured illumination","authors":"Chang Qiao, Hui Qiao, Jiamin Wu, Xiaoxu Li, Jingtao Fan, Qionghai Dai","doi":"10.1364/ntm.2019.nm3c.3","DOIUrl":"https://doi.org/10.1364/ntm.2019.nm3c.3","url":null,"abstract":"Deep learning based tomographic phase microscopy with blind structured illumination (DeepTomo) is a unique imaging mechanism that combines simplified optical design with deep neural networks. The simulation results show that DeepTomo has high spatiotemporal resolution.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81047131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BRAIN.2019.BW4A.7
M. Menzel, M. Axer, K. Amunts, H. Raedt, K. Michielsen
We explore the polarization-(in)dependent transmitted light intensity of histological brain sections. Using experimental and simulation studies, we demonstrate that it contains valuable information about nerve fiber architecture and tissue structure.
{"title":"Transmittance and Diattenuation Measurements Reveal Different Properties of Brain Tissue","authors":"M. Menzel, M. Axer, K. Amunts, H. Raedt, K. Michielsen","doi":"10.1364/BRAIN.2019.BW4A.7","DOIUrl":"https://doi.org/10.1364/BRAIN.2019.BW4A.7","url":null,"abstract":"We explore the polarization-(in)dependent transmitted light intensity of histological brain sections. Using experimental and simulation studies, we demonstrate that it contains valuable information about nerve fiber architecture and tissue structure.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"249 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73528113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-14DOI: 10.1364/BODA.2019.DT3B.6
Paulina Niedźwiedziuk, K. Karnowski, M. Wojtkowski
Lung cancer cells were measured in scanning Optical Coherence Microscopy setup. Consecutive 2D speckled data was decorrelated to obtain flow information. We observed regions which are more active than others.
{"title":"Speckle decorrelation for cell’s dynamics","authors":"Paulina Niedźwiedziuk, K. Karnowski, M. Wojtkowski","doi":"10.1364/BODA.2019.DT3B.6","DOIUrl":"https://doi.org/10.1364/BODA.2019.DT3B.6","url":null,"abstract":"Lung cancer cells were measured in scanning Optical Coherence Microscopy setup. Consecutive 2D speckled data was decorrelated to obtain flow information. We observed regions which are more active than others.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82100535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lu Zhi, Jiamin Wu, Hui Qiao, Tao Yan, Zi-Ye Zhou, Xu Zhang, Jingtao Fan, Qionghai Dai
We propose an artifact-free deconvolution method for light field microscopy by ptychographic iterations in phase space. Experiments on biological samples show the resolution enhancement with much less artifacts and computational cost.
{"title":"Artifact-free 3D deconvolution for light field microscopy","authors":"Lu Zhi, Jiamin Wu, Hui Qiao, Tao Yan, Zi-Ye Zhou, Xu Zhang, Jingtao Fan, Qionghai Dai","doi":"10.1364/NTM.2019.NS1B.2","DOIUrl":"https://doi.org/10.1364/NTM.2019.NS1B.2","url":null,"abstract":"We propose an artifact-free deconvolution method for light field microscopy by ptychographic iterations in phase space. Experiments on biological samples show the resolution enhancement with much less artifacts and computational cost.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88006294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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