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Inorganic salt starvation improves the polysaccharide production and CO2 fixation by Porphyridium purpureum. 无机盐饥饿可提高紫卟啉的多糖产量和二氧化碳固定能力。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-07-01 Epub Date: 2024-05-13 DOI: 10.1007/s00449-024-03017-0
Yinchen Li, Shengshan Wu, Haowei Chen, Wupeng Xiao, Chuang Li, Zhiqing Peng, Zheng Li, Jian Liu, Lu Lin, Xianhai Zeng

The microalgae industry shows a promising future in the production of high-value products such as pigments, phycoerythrin, polyunsaturated fatty acids, and polysaccharides. It was found that polysaccharides have high biomedical value (such as antiviral, antibacterial, antitumor, antioxidative) and industrial application prospects (such as antioxidants). This study aimed to improve the polysaccharides accumulation of Porphyridium purpureum CoE1, which was effectuated by inorganic salt starvation strategy whilst supplying rich carbon dioxide. At a culturing temperature of 25 °C, the highest polysaccharide content (2.89 g/L) was achieved in 50% artificial seawater on the 12th day. This accounted for approximately 37.29% of the dry biomass, signifying a 25.3% increase in polysaccharide production compared to the culture in 100% artificial seawater. Subsequently, separation, purification and characterization of polysaccharides produced were conducted. Furthermore, the assessment of CO2 fixation capacity during the cultivation of P. purpureum CoE1 was conducted in a 10 L photobioreactor. This indicated that the strain exhibited an excellent CO2 fixation capacity of 1.66 g CO2/g biomass/d. This study proposed an efficient and feasible approach that not only increasing the yield of polysaccharides by P. purpureum CoE1, but also fixing CO2 with a high rate, which showed great potential in the microalgae industry and Bio-Energy with Carbon Capture and Storage.

微藻产业在生产颜料、藻红素、多不饱和脂肪酸和多糖等高价值产品方面前景广阔。研究发现,多糖具有很高的生物医学价值(如抗病毒、抗菌、抗肿瘤、抗氧化)和工业应用前景(如抗氧化剂)。本研究旨在通过无机盐饥饿策略,同时提供丰富的二氧化碳,提高紫斑卟啉 CoE1 的多糖积累。在 25 °C 的培养温度下,第 12 天,50% 的人工海水中的多糖含量最高(2.89 克/升)。多糖含量约占干生物量的 37.29%,与在 100% 人工海水中培养相比,多糖产量增加了 25.3%。随后,对产生的多糖进行了分离、纯化和表征。此外,还在 10 L 光生物反应器中评估了 P. purpureum CoE1 培养过程中的二氧化碳固定能力。结果表明,该菌株具有出色的二氧化碳固定能力,达到 1.66 g CO2/g 生物质/天。该研究提出了一种高效可行的方法,不仅能提高紫苏藻 CoE1 的多糖产量,还能高速固定二氧化碳,在微藻产业和碳捕集与封存生物能源方面具有巨大潜力。
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引用次数: 0
Hybrid modeling of an ultracentrifugation process for separation of full and empty adeno-associated virus particles. 超速离心过程的混合建模,用于分离完整和空的腺相关病毒颗粒。
IF 3.8 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-06-01 Epub Date: 2024-05-04 DOI: 10.1007/s00449-024-03014-3
Riccardo De-Luca, Miguel Pupo-Correia, Michael Feldhofer, Duarte L Martins, Alexandra Umprecht, Ali Shahmohammadi, Daniel Corona, Moritz von Stosch

Ultracentrifugation is an attractive method for separating full and empty capsids, exploiting their density difference. Changes of the serotype/capsid, density of loading material, or the genetic information contained in the adeno-associated viruses (AAVs) require the adaptation of the harvesting parameters and the density gradient loaded onto the centrifuge. To streamline these adaptations, a mathematical model could support the design and testing of operating conditions.Here, hybrid models, which combine empirical functions with artificial neural networks, are proposed to describe the separation of full and empty capsids as a function of material and operational parameters, i.e., the harvest model. In addition, critical quality attributes are estimated by a quality model which is operating on top of the harvest model. The performance of these models was evaluated using test data and two additional blind runs. Also, a "what-if" analysis was conducted to investigate whether the models' predictions align with expectations.It is concluded that the models are sufficiently accurate to support the design of operating conditions, though the accuracy and applicability of the models can further be increased by training them on more specific data with higher variability.

超速离心是一种很有吸引力的方法,可利用其密度差来分离全壳和空壳。要改变血清型/囊壳、装载材料的密度或腺病毒(AAV)所含的遗传信息,就必须调整离心机的收获参数和装载的密度梯度。为了简化这些调整,数学模型可为操作条件的设计和测试提供支持。在此,我们提出了将经验函数与人工神经网络相结合的混合模型,以描述作为材料和操作参数(即收获模型)函数的全壳和空壳分离。此外,关键质量属性是通过在收获模型基础上运行的质量模型来估算的。利用测试数据和两次额外的盲运行对这些模型的性能进行了评估。此外,还进行了 "假设 "分析,以研究这些模型的预测是否与预期一致。结论是,这些模型的准确性足以支持操作条件的设计,尽管可以通过对变异性更高的特定数据进行训练来进一步提高模型的准确性和适用性。
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引用次数: 0
Optimization of fermentation conditions for the production of γ-aminobutyric acid by Lactobacillus hilgardii GZ2 from traditional Chinese fermented beverage system. 优化中国传统发酵饮料体系中希尔加德乳杆菌 GZ2 生产γ-氨基丁酸的发酵条件。
IF 3.8 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-06-01 Epub Date: 2024-05-08 DOI: 10.1007/s00449-024-03028-x
Xiao-Zhou Zou, Lu-Chan Gong, Ting-Ting Li, Shu-Yi Lv, Jun Wang

γ-Aminobutyric acid (GABA) is a crucial neurotransmitter with wide application prospects. In this study, we focused on a GABA-producing strain from a traditional Chinese fermented beverage system. Among the six isolates, Lactobacillus hilgardii GZ2 exhibited the greatest ability to produce GABA in the traditional Chinese fermented beverage system. To increase GABA production, we optimized carbon sources, nitrogen sources, temperature, pH, and monosodium glutamate and glucose concentrations and conducted fed-batch fermentation. The best carbon and nitrogen sources for GABA production and cell growth were glucose, yeast extract and tryptone. Gradual increases in GABA were observed as the glucose and monosodium glutamate concentrations increased from 10 g/L to 50 g/L. During fed-batch fermentation, lactic acid was used to maintain the pH at 5.56, and after feeding with 0.03 g/mL glucose and 0.4 g/mL sodium glutamate for 72 h, the GABA yield reached 239 g/L. This novel high-GABA-producing strain holds great potential for the industrial production of GABA, as well as the development of health-promoting functional foods and medical fields.

γ-氨基丁酸(GABA)是一种重要的神经递质,具有广泛的应用前景。在本研究中,我们重点研究了中国传统发酵饮料系统中产生 GABA 的菌株。在六个分离株中,希尔加德乳杆菌 GZ2 在中国传统发酵饮料系统中生产 GABA 的能力最强。为了提高 GABA 的产量,我们对碳源、氮源、温度、pH 值、谷氨酸钠和葡萄糖的浓度进行了优化,并进行了喂料批次发酵。葡萄糖、酵母提取物和胰蛋白胨是生产 GABA 和促进细胞生长的最佳碳源和氮源。随着葡萄糖和谷氨酸钠浓度从 10 克/升增加到 50 克/升,观察到 GABA 逐渐增加。在饲料批量发酵过程中,使用乳酸将 pH 值保持在 5.56,在添加 0.03 克/毫升葡萄糖和 0.4 克/毫升谷氨酸钠 72 小时后,GABA 产量达到 239 克/升。这种高产 GABA 的新型菌株在 GABA 的工业化生产以及促进健康的功能食品和医疗领域的开发方面具有巨大潜力。
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引用次数: 0
Comparative study of the removal of sulfate by UASB in light and dark environment. UASB 在明暗环境中去除硫酸盐的比较研究。
IF 3.8 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-06-01 Epub Date: 2024-05-04 DOI: 10.1007/s00449-024-03024-1
Yuanyao Ye, Xueyi Yan, Hui Luo, Jianxiong Kang, Dongqi Liu, Yongzheng Ren, Huu Hao Ngo, Wenshan Guo, Dongle Cheng, Wei Jiang

At present, the application of sewage treatment technologies is restricted by high sulfate concentrations. In the present work, the sulfate removal was biologically treated using an upflow anaerobic sludge blanket (UASB) in the absence/presence of light. First, the start-up of UASB for the sulfate removal was studied in terms of COD degradation, sulfate removal, and effluent pH. Second, the impacts of different operation parameters (i.e., COD/SO42- ratio, temperature and illumination time) on the UASB performance were explored. Third, the properties of sludge derived from the UASB at different time were analyzed. Results show that after 28 days of start-up, the COD removal efficiencies in both the photoreactor and non-photoreactor could reach a range of 85-90% while such reactors could achieve > 90% of sulfate being removed. Besides, higher illumination time could facilitate the removal of pollutants in the photoreactor. To sum up, the present study can provide technical support for the clean removal of sulfate from wastewater using photoreactors.

目前,污水处理技术的应用受到高浓度硫酸盐的限制。在本研究中,利用上流式厌氧污泥毯(UASB)在无光/有光条件下对硫酸盐的去除进行了生物处理。首先,从化学需氧量降解、硫酸盐去除和出水 pH 值方面研究了 UASB 在去除硫酸盐方面的启动情况。其次,探讨了不同运行参数(即 COD/SO42- 比率、温度和光照时间)对 UASB 性能的影响。第三,分析了 UASB 在不同时间产生的污泥性质。结果表明,在启动 28 天后,光反应器和非光反应器的 COD 去除率均可达到 85%-90% 的范围,而此类反应器的硫酸盐去除率可达到 90% 以上。此外,光照时间越长,光反应器中污染物的去除率越高。总之,本研究可为利用光反应器清洁去除废水中的硫酸盐提供技术支持。
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引用次数: 0
Synthesis, characterisation and in vitro anticancer activity of conjugated protease inhibitor-silver nanoparticles (AgNPs-PI) against human breast MCF-7 and prostate PC-3 cancer cell lines. 共轭蛋白酶抑制剂-银纳米粒子(AgNPs-PI)的合成、表征及对人类乳腺癌 MCF-7 和前列腺癌 PC-3 细胞株的体外抗癌活性。
IF 3.8 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-06-01 Epub Date: 2024-05-06 DOI: 10.1007/s00449-024-03023-2
Kiran Marathe, Jitendra Naik, Vijay Maheshwari

The conjugated silver nanoparticles using biomolecules have attracted great attention of researchers because physical dimensions and surface chemistry play important roles in toxicity and biocompatibility of AgNPs. Hence, in the current study, synthesis of bio-conjugated AgNPs with protein protease inhibitor (PI) isolated from Streptomyces spp. is reported. UV-visible spectra of PI and AgNPs showed stronger peaks at 280 and 405 nm, confirming the synthesis of conjugated AgNPs-PI. TEM and SEM images of AgNPs-PI showed spherical-shaped nanoparticles with a slight increase in particle size and thin amorphous layer around the surface of silver nanomaterial. Circular dichroism, FT-IR and fluorescence spectral studies confirmed AgNPs-PI conjugation. Conjugated AgNPs-PI showed excellent anticancer potential than AgNPs and protease inhibitor separately on human breast MCF-7 and prostate PC-3 cell lines. The findings revealed that surface modification of AgNPs with protein protease inhibitor stabilised the nanomaterial and increased its anticancer activity.

由于物理尺寸和表面化学性质对银纳米粒子的毒性和生物相容性起着重要作用,因此利用生物大分子共轭银纳米粒子引起了研究人员的极大关注。因此,本研究报道了用从链霉菌中分离出的蛋白酶抑制剂(PI)合成生物共轭 AgNPs。PI 和 AgNPs 的紫外可见光谱在 280 纳米和 405 纳米处显示出较强的峰值,证实了 AgNPs-PI 的合成。AgNPs-PI 的 TEM 和 SEM 图像显示其为球形纳米颗粒,粒径略有增大,银纳米材料表面有一层薄薄的无定形层。圆二色性、傅立叶变换红外光谱和荧光光谱研究证实了 AgNPs-PI 的共轭作用。与分别使用 AgNPs 和蛋白酶抑制剂相比,共轭 AgNPs-PI 对人类乳腺 MCF-7 和前列腺 PC-3 细胞株显示出卓越的抗癌潜力。研究结果表明,用蛋白酶抑制剂对 AgNPs 进行表面修饰可稳定纳米材料并提高其抗癌活性。
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引用次数: 0
Trichoderma koningiopsis fermentation in airlift bioreactor for bioherbicide production. 在气升式生物反应器中发酵小米糠毛霉以生产生物除草剂。
IF 3.8 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 Epub Date: 2024-03-30 DOI: 10.1007/s00449-024-02991-9
Aline Frumi Camargo, Simone Kubeneck, Charline Bonatto, Suzana Fátima Bazoti, Júlia Pieper Nerling, Gabriel Henrique Klein, William Michelon, Sérgio L Alves, Altemir José Mossi, Gislaine Fongaro, Helen Treichel

During scaling of fermentations, choosing a bioreactor is fundamental to ensure the product's quality. This study aims to produce bioherbicides using Trichoderma koningiopsis fermentation, evaluating process parameters in an Airlift bioreactor. As a response, we quantified the production of enzymes involved in the bioherbicide activity (amylase, cellulase, laccase, lipase, and peroxidase). In addition, it evaluated the agronomic efficiency of the fermented extract optimized through tests that promoted soybean growth and nodulation, soybean seed germination, and in vitro phytopathogen control. As a result of optimizing the scaling bioprocess, it was possible to obtain an adequate fermentation condition, which, when applied to soybean seeds, had beneficial effects on their growth. It allowed the production of an enzyme cocktail. These results add a crucial biotechnological potential factor for the success of the optimized formulation in the Airlift bioreactor, in addition to presenting relevant results for the scientific community.

在扩大发酵规模的过程中,选择生物反应器是确保产品质量的关键。本研究旨在利用科宁氏毛霉发酵法生产生物除草剂,评估气升式生物反应器的工艺参数。作为回应,我们对参与生物除草剂活性的酶(淀粉酶、纤维素酶、漆酶、脂肪酶和过氧化物酶)的产量进行了量化。此外,我们还通过促进大豆生长和结瘤、大豆种子发芽以及体外植物病原体控制等试验,评估了发酵提取物的农艺效率。通过优化缩放生物工艺,获得了适当的发酵条件。它允许生产一种鸡尾酒酶。除了向科学界展示相关成果外,这些成果还为空运生物反应器中优化配方的成功增加了一个重要的生物技术潜在因素。
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引用次数: 0
The function of microbial enzymes in breaking down soil contaminated with pesticides: a review. 微生物酶在分解受农药污染的土壤中的功能:综述。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 Epub Date: 2024-03-08 DOI: 10.1007/s00449-024-02978-6
Xing Kai Chia, Tony Hadibarata, Risky Ayu Kristanti, Muhammad Noor Hazwan Jusoh, Inn Shi Tan, Henry Chee Yew Foo

The use of pesticides and the subsequent accumulation of residues in the soil has become a worldwide problem. Organochlorine (OC) pesticides have spread widely in the environment and caused contamination from past agricultural activities. This article reviews the bioremediation of pesticide compounds in soil using microbial enzymes, including the enzymatic degradation pathway and the recent development of enzyme-mediated bioremediation. Enzyme-mediated bioremediation is divided into phase I and phase II, where the former increases the solubility of pesticide compounds through oxidation-reduction and hydrolysis reactions, while the latter transforms toxic pollutants into less toxic or nontoxic products through conjugation reactions. The identified enzymes that can degrade OC insecticides include dehalogenases, phenol hydroxylase, and laccases. Recent developments to improve enzyme-mediated bioremediation include immobilization, encapsulation, and protein engineering, which ensure its stability, recyclability, handling and storage, and better control of the reaction.

农药的使用及其在土壤中的残留积累已成为一个世界性问题。有机氯(OC)杀虫剂在环境中广泛传播,并在过去的农业活动中造成污染。本文综述了利用微生物酶对土壤中的农药化合物进行生物修复的情况,包括酶降解途径和酶介导生物修复的最新发展。酶介导生物修复分为第一阶段和第二阶段,前者通过氧化还原和水解反应增加农药化合物的溶解度,后者通过共轭反应将有毒污染物转化为毒性较低或无毒的产物。已发现的可降解 OC 杀虫剂的酶包括脱卤酶、苯酚羟化酶和裂解酶。最近在改进酶介导的生物修复方面取得的进展包括固定化、封装和蛋白质工程,这确保了酶的稳定性、可回收性、处理和储存,以及对反应的更好控制。
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引用次数: 0
Improved production of RNA-inhibiting antimicrobial peptide by Bacillus licheniformis MCC 2514 facilitated by a genetic algorithm optimized medium. 遗传算法优化培养基促进地衣芽孢杆菌 MCC 2514 生产 RNA 抑制抗菌肽。
IF 3.8 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 Epub Date: 2024-03-23 DOI: 10.1007/s00449-024-02998-2
Ishrat Jahan Peerzade, Sarma Mutturi, Prakash M Halami

One of the significant challenges during the purification and characterization of antimicrobial peptides (AMPs) from Bacillus sp. is the interference of unutilized peptides from complex medium components during analytical procedures. In this study, a semi-synthetic medium was devised to overcome this challenge. Using a genetic algorithm, the production medium of AMP is optimized. The parent organism, Bacillus licheniformis MCC2514, produces AMP in very small quantities. This AMP is known to inhibit RNA biosynthesis. The findings revealed that lactose, NH4Cl and NaNO3 were crucial medium constituents for enhanced AMP synthesis. The potency of the AMP produced was studied using bacterium, Kocuria rhizophila ATCC 9341. The AMP produced from the optimized medium was eightfold higher than that produced from the unoptimized medium. Furthermore, activity was increased by 1.5-fold when cultivation conditions were standardized using the optimized medium. Later, AMP was produced in a 5 L bioreactor under controlled conditions, which led to similar results as those of shake-flask production. The mode of action of optimally produced AMP was confirmed to be inhibition of RNA biosynthesis. Here, we demonstrate that improved production of AMP is possible with the developed semi-synthetic medium recipe and could help further AMP production in an industrial setup.

在纯化和表征芽孢杆菌抗菌肽(AMPs)的过程中,面临的重大挑战之一是分析过程中复杂培养基成分对未利用肽的干扰。本研究设计了一种半合成培养基来克服这一难题。利用遗传算法优化了 AMP 的生产培养基。母体地衣芽孢杆菌 MCC2514 能产生极少量的 AMP。众所周知,这种 AMP 能抑制 RNA 的生物合成。研究结果表明,乳糖、NH4Cl 和 NaNO3 是增强 AMP 合成的关键培养基成分。使用 Kocuria rhizophila ATCC 9341 细菌研究了所产生的 AMP 的效力。优化培养基产生的 AMP 是未优化培养基的八倍。此外,当使用优化培养基对培养条件进行标准化后,其活性提高了 1.5 倍。随后,在受控条件下,在 5 升生物反应器中生产 AMP,结果与摇瓶生产类似。经证实,优化生产的 AMP 的作用模式是抑制 RNA 的生物合成。在此,我们证明了使用所开发的半合成培养基配方可以提高 AMP 的产量,并有助于在工业装置中进一步生产 AMP。
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引用次数: 0
Analyzing of hydrodynamic stress and mass transfer requirements of a fermentation process carried out in a coaxial bioreactor: a scale-up study. 分析在同轴生物反应器中进行发酵过程的流体动力应力和传质要求:规模扩大研究。
IF 3.8 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 Epub Date: 2024-04-01 DOI: 10.1007/s00449-024-02990-w
Ali Rahimzadeh, Farhad Ein-Mozaffari, Ali Lohi

Fluid hydrodynamic stress has a deterministic effect on the morphology of filamentous fungi. Although the coaxial mixer has been recognized as a suitable gas dispersion system for minimizing inhomogeneities within a bioreactor, its performance for achieving enhanced oxygen transfer while operating at a reduced shear environment has not been investigated yet, specifically upon scale-up. Therefore, the influence of the impeller type, aeration rate, and central impeller retrofitting on the efficacy of an abiotic coaxial system containing a shear-thinning fluid was examined. The aim was to assess the hydrodynamic parameters, including stress, mass transfer, bubble size, and gas hold-up, upon conducting a scale-up study. The investigation was conducted through dynamic gassing-in, tomography, and computational fluid dynamics combined with population balance methods. It was observed that the coaxial bioreactor performance was strongly influenced by the agitator type. In addition, coaxial bioreactors are scalable in terms of shear environment and oxygen transfer rate.

流体流体动力对丝状真菌的形态具有决定性影响。虽然同轴混合器已被认为是一种合适的气体分散系统,可最大限度地减少生物反应器内的不均匀性,但其在减少剪切力的环境中运行时增强氧气转移的性能尚未得到研究,特别是在放大时。因此,我们研究了叶轮类型、曝气速率和中央叶轮改装对含有剪切稀化流体的非生物同轴系统功效的影响。目的是在进行放大研究时评估流体力学参数,包括应力、传质、气泡大小和气体截留。研究通过动态进气、层析成像和计算流体力学结合种群平衡方法进行。研究发现,同轴生物反应器的性能受搅拌器类型的影响很大。此外,同轴生物反应器在剪切环境和氧气转移率方面具有可扩展性。
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引用次数: 0
Correction: The function of microbial enzymes in breaking down soil contaminated with pesticides: a review. 更正:微生物酶在分解受农药污染的土壤中的功能:综述。
IF 3.8 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 DOI: 10.1007/s00449-024-03026-z
Xing Kai Chia, Tony Hadibarata, Risky Ayu Kristanti, Muhammad Noor Hazwan Jusoh, Inn Shi Tan, Henry Chee Yew Foo
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引用次数: 0
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Bioprocess and Biosystems Engineering
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