首页 > 最新文献

Bioprocess and Biosystems Engineering最新文献

英文 中文
Phyto-fabricated ZnO nanoparticles for anticancer, photo-antimicrobial effect on carbapenem-resistant/sensitive Pseudomonas aeruginosa and removal of tetracycline. 用于抗癌、对碳青霉烯耐药/敏感铜绿假单胞菌的光抗菌作用以及去除四环素的植物制备氧化锌纳米粒子。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-16 DOI: 10.1007/s00449-024-02984-8
Gopinath Venkatraman, Priyadarshini Sakthi Mohan, Maryam Mohammed Mashghan, Kar-Cheng Wong, Puteri Shafinaz Abdul-Rahman, Kumutha Malar Vellasamy, Abdurahman Hajinur Hirad, Abdullah A Alarfaj, Shifa Wang

Alternanthera sessilis (AS) leaf extract was used to synthesize zinc oxide nanoparticles (ZnO NPs). Bioanalytical characterization techniques such as X-ray diffraction (XRD) and field emission scanning electron microscope (FESEM) confirmed the formation of crystalline ZnO NPs with average sizes of 40 nm. The AS-ZnO NPs antimicrobial activity was analyzed under dark (D) and white light (WL) conditions. The improved antimicrobial activity was observed against Escherichia coli, Staphylococcus aureus and Bacillus subtilis at the minimal inhibitory concentration (MIC) of 125 and 62.5 µg/mL under WL than the D at 125 and 250 µg/mL for E. coli, B. subtilis, and Pseudomonas aeruginosa, respectively. In contrast, the growth of P. aeruginosa and S. aureus was not completely inhibited until 1 mg/mL AS-ZnO NPs under WL and D. Similarly, AS-ZnO NPs displayed a weaker inhibitory effect against carbapenem-sensitive P. aeruginosa (CSPA) and carbapenem-resistant P. aeruginosa (CRPA) strains of PAC023, PAC041 and PAC032, PAC045 under D. Interestingly, the distinct inhibitory effect was recorded against CSPA PAC041 and CRPA PAC032 in which the bacteria growth was inhibited 99.9% at 250, 500 µg/mL under WL. The cytotoxicity results suggested AS-ZnO NPs demonstrated higher toxicity to MCF-7 breast cancer cells than the RAW264.7 macrophage cells. Further, AS-ZnO NPs exhibited higher catalytic potential against tetracycline hydrochloride (TC-H) degradation at 65.6% and 60.8% under WL than the dark at 59.35% and 48.6% within 120 min. Therefore, AS-ZnO NPs can be used to design a photo-improved antimicrobial formulation and environmental catalyst for removing TC-H from wastewater.

Alternanthera sessilis(AS)叶提取物被用来合成氧化锌纳米粒子(ZnO NPs)。X 射线衍射(XRD)和场发射扫描电子显微镜(FESEM)等生物分析表征技术证实了平均尺寸为 40 nm 的结晶 ZnO NPs 的形成。在黑暗(D)和白光(WL)条件下分析了 AS-ZnO NPs 的抗菌活性。对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的最小抑菌浓度(MIC)分别为 125 微克/毫升和 62.5 微克/毫升时,在 WL 条件下,AS-ZnO NPs 的抗菌活性比在 D 条件下的 125 微克/毫升和 250 微克/毫升有所提高。同样,AS-ZnO NPs 对碳青霉烯敏感的铜绿假单胞菌(CSPA)和碳青霉烯耐药的铜绿假单胞菌(CRPA)的抑制作用也较弱。有趣的是,As-ZnO NPs 对碳青霉烯敏感的铜绿假单胞菌(CSPA)和碳青霉烯耐药的铜绿假单胞菌(CRPA)菌株 PAC023、PAC041 和 PAC032、PAC045 有明显的抑制作用。细胞毒性结果表明,AS-ZnO NPs 对 MCF-7 乳腺癌细胞的毒性高于 RAW264.7 巨噬细胞。此外,在 120 分钟内,AS-ZnO NPs 在 WL 下对盐酸四环素(TC-H)降解的催化潜力分别为 65.6% 和 60.8%,高于黑暗条件下的 59.35% 和 48.6%。因此,AS-ZnO NPs 可用于设计一种光改进的抗菌配方和环境催化剂,以去除废水中的 TC-H。
{"title":"Phyto-fabricated ZnO nanoparticles for anticancer, photo-antimicrobial effect on carbapenem-resistant/sensitive Pseudomonas aeruginosa and removal of tetracycline.","authors":"Gopinath Venkatraman, Priyadarshini Sakthi Mohan, Maryam Mohammed Mashghan, Kar-Cheng Wong, Puteri Shafinaz Abdul-Rahman, Kumutha Malar Vellasamy, Abdurahman Hajinur Hirad, Abdullah A Alarfaj, Shifa Wang","doi":"10.1007/s00449-024-02984-8","DOIUrl":"10.1007/s00449-024-02984-8","url":null,"abstract":"<p><p>Alternanthera sessilis (AS) leaf extract was used to synthesize zinc oxide nanoparticles (ZnO NPs). Bioanalytical characterization techniques such as X-ray diffraction (XRD) and field emission scanning electron microscope (FESEM) confirmed the formation of crystalline ZnO NPs with average sizes of 40 nm. The AS-ZnO NPs antimicrobial activity was analyzed under dark (D) and white light (WL) conditions. The improved antimicrobial activity was observed against Escherichia coli, Staphylococcus aureus and Bacillus subtilis at the minimal inhibitory concentration (MIC) of 125 and 62.5 µg/mL under WL than the D at 125 and 250 µg/mL for E. coli, B. subtilis, and Pseudomonas aeruginosa, respectively. In contrast, the growth of P. aeruginosa and S. aureus was not completely inhibited until 1 mg/mL AS-ZnO NPs under WL and D. Similarly, AS-ZnO NPs displayed a weaker inhibitory effect against carbapenem-sensitive P. aeruginosa (CSPA) and carbapenem-resistant P. aeruginosa (CRPA) strains of PAC023, PAC041 and PAC032, PAC045 under D. Interestingly, the distinct inhibitory effect was recorded against CSPA PAC041 and CRPA PAC032 in which the bacteria growth was inhibited 99.9% at 250, 500 µg/mL under WL. The cytotoxicity results suggested AS-ZnO NPs demonstrated higher toxicity to MCF-7 breast cancer cells than the RAW264.7 macrophage cells. Further, AS-ZnO NPs exhibited higher catalytic potential against tetracycline hydrochloride (TC-H) degradation at 65.6% and 60.8% under WL than the dark at 59.35% and 48.6% within 120 min. Therefore, AS-ZnO NPs can be used to design a photo-improved antimicrobial formulation and environmental catalyst for removing TC-H from wastewater.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140139639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green synthesis of copper nanoparticles by using pineapple peel waste: in vitro characterizations and antibacterial potential. 利用菠萝皮废弃物绿色合成纳米铜粒子:体外表征和抗菌潜力。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-27 DOI: 10.1007/s00449-024-02982-w
Sanchita Mitra, Tarun Kumar Dua, Serina Easmin, Sucheta Sarkar, Arijit Prosad Roy, Ranabir Sahu, Gouranga Nandi, Md Salman Haydar, Swarnendu Roy, Paramita Paul

A considerable amount of fruit waste is being produced every day worldwide. The green synthesis of metal nanoparticles from fruit peel waste can be an innovative, cost-effective, and eco-friendly alternative to traditional methods. Copper nanoparticles (CuNPs) were synthesized by a green method using the pineapple peels extract (PLX) and copper sulfate pentahydrate. The formation of CuNPs was visually identified and detected by UV-Visible spectroscopy. The CuNPs were characterized by Fourier-transform infrared (FTIR) spectroscopy, particle size analyzer, scanning electron microscopy (SEM), and X-ray diffraction (XRD). The antioxidant and reducing power of CuNPs were conducted by %DPPH scavenging and electron transfer-based ferric reducing antioxidant power (FRAP) assay, respectively. The antibacterial properties of CuNPs were determined in gram-positive, and gram-negative bacteria. The results showed that the CuNPs were spherical in shape with mean particle size 290.5 nm. The zeta potential of the nanoparticles was found to be - 12.3 mV indicating the instability in the colloidal state. The FTIR study confirmed the peaks of phytochemicals present in the PLX and the nanoparticles supporting the use of pineapple peels as stabilizing, reducing and capping agents. Both the DPPH and reducing power assay depicted that the synthesized CuNPs had significant antioxidant activity. However, the synthesized CuNPs had strong inhibitory capacity against both gram-positive and gram-negative test organisms. Thus, the CuNPS could be used for its viable antibacterial potential to preserve fruits, flowers, and vegetables from bacterial contamination.

全世界每天都会产生大量的水果废料。利用果皮废弃物绿色合成金属纳米粒子是一种创新、经济、环保的方法,可替代传统方法。本研究采用菠萝果皮提取物(PLX)和五水硫酸铜,以绿色方法合成了纳米铜粒子(CuNPs)。CuNPs 的形成可通过肉眼识别,并可通过紫外可见光谱检测。傅立叶变换红外光谱(FTIR)、粒度分析仪、扫描电子显微镜(SEM)和 X 射线衍射(XRD)对 CuNPs 进行了表征。CuNPs 的抗氧化性和还原力分别通过%DPPH 清除率和基于电子传递的铁还原抗氧化力(FRAP)测定法进行了检测。还测定了 CuNPs 在革兰氏阳性菌和革兰氏阴性菌中的抗菌特性。结果表明,CuNPs 呈球形,平均粒径为 290.5 nm。纳米颗粒的 zeta 电位为 - 12.3 mV,表明其胶体状态不稳定。傅立叶变换红外光谱(FTIR)研究证实了 PLX 和纳米颗粒中存在的植物化学物质峰,支持使用菠萝皮作为稳定剂、还原剂和封盖剂。DPPH 和还原力分析表明,合成的 CuNPs 具有显著的抗氧化活性。不过,合成的 CuNPs 对革兰氏阳性和阴性试验生物都有很强的抑制能力。因此,CuNPS 具有可行的抗菌潜力,可用于保存水果、鲜花和蔬菜,使其免受细菌污染。
{"title":"Green synthesis of copper nanoparticles by using pineapple peel waste: in vitro characterizations and antibacterial potential.","authors":"Sanchita Mitra, Tarun Kumar Dua, Serina Easmin, Sucheta Sarkar, Arijit Prosad Roy, Ranabir Sahu, Gouranga Nandi, Md Salman Haydar, Swarnendu Roy, Paramita Paul","doi":"10.1007/s00449-024-02982-w","DOIUrl":"10.1007/s00449-024-02982-w","url":null,"abstract":"<p><p>A considerable amount of fruit waste is being produced every day worldwide. The green synthesis of metal nanoparticles from fruit peel waste can be an innovative, cost-effective, and eco-friendly alternative to traditional methods. Copper nanoparticles (CuNPs) were synthesized by a green method using the pineapple peels extract (PLX) and copper sulfate pentahydrate. The formation of CuNPs was visually identified and detected by UV-Visible spectroscopy. The CuNPs were characterized by Fourier-transform infrared (FTIR) spectroscopy, particle size analyzer, scanning electron microscopy (SEM), and X-ray diffraction (XRD). The antioxidant and reducing power of CuNPs were conducted by %DPPH scavenging and electron transfer-based ferric reducing antioxidant power (FRAP) assay, respectively. The antibacterial properties of CuNPs were determined in gram-positive, and gram-negative bacteria. The results showed that the CuNPs were spherical in shape with mean particle size 290.5 nm. The zeta potential of the nanoparticles was found to be - 12.3 mV indicating the instability in the colloidal state. The FTIR study confirmed the peaks of phytochemicals present in the PLX and the nanoparticles supporting the use of pineapple peels as stabilizing, reducing and capping agents. Both the DPPH and reducing power assay depicted that the synthesized CuNPs had significant antioxidant activity. However, the synthesized CuNPs had strong inhibitory capacity against both gram-positive and gram-negative test organisms. Thus, the CuNPS could be used for its viable antibacterial potential to preserve fruits, flowers, and vegetables from bacterial contamination.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140304771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biogenic silver nanoparticle synthesis using orange peel extract and its multifaceted biomedical application. 利用橙皮提取物合成生物银纳米粒子及其多方面的生物医学应用。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1007/s00449-024-03031-2
Umme Hani, Fawziah Nasser Kidwan, Lamis Ahmed Albarqi, Saud Abduluziz Al-Qahtani, Ruba Muhammad AlHadi, Haifa Abdullah AlZaid, Nazima Haider, Mohammad Azam Ansari

The aim of this study was to employ an agro-industrial byproduct, specifically Citrus sinensis peels, as a reservoir of polyphenols. The natural chemicals present in C. sinensis peels serve as reducing agents in an environmentally benign method for synthesizing silver nanoparticles (AgNPs). This methodology not only provides a more environmentally friendly method for synthesizing nanoparticles but also enhances the value of agricultural waste, emphasizing the sustainable utilization of resources. In our study, AgNPs were successfully synthesized using peel aqueous exact of C. sinensis and then their various biological activity has been investigated. The synthesized AgNPs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and transmission electron microscopy (TEM) analysis. Furthermore, their effectiveness in inhibiting growth and biofilm formation of Escherichia coli, Staphylococcus aureus, and Candida albicans has been investigated. The minimum inhibitory concentrations (MIC) for E. coli and S. aureus were both 32 μg/mL, and for C. albicans, it was 128 µg/mL. At 250 µg/mL of AgNPs, 94% and 92% biofilm inhibition were observed against E. coli and S. aureus, respectively. Furthermore, AgNPs demonstrated significant toxic effects against human prostate cancer cell line DU145 as investigated by anti-apoptotic, 4',6-diamidino-2-phenylindole (DAPI), reactive oxygen species (ROS), and acridine orange/ethidium bromide (AO/EtBr) assays. We also conducted uptake analysis on these pathogens and cancer cell lines to preliminarily investigate the mechanisms underlying their toxic effects. These findings confirm that AgNPs can serve as a cost-effective, non-toxic, and environmentally friendly resource for green synthesis of medicinal AgNPs. Moreover, this approach offers an alternative recycling strategy that contributes to the sustainable use of biological by-products.

本研究的目的是利用一种农用工业副产品,特别是柑橘皮,作为多酚的宝库。柑橘皮中的天然化学物质可作为还原剂,以一种对环境无害的方法合成银纳米粒子(AgNPs)。这种方法不仅提供了一种更环保的合成纳米粒子的方法,还提高了农业废弃物的价值,强调了资源的可持续利用。在我们的研究中,我们成功地利用去皮水溶液合成了 AgNPs,并对其各种生物活性进行了研究。通过紫外-可见光谱、动态光散射(DLS)、扫描电子显微镜(SEM)、能量色散 X 射线(EDX)和透射电子显微镜(TEM)分析,对合成的 AgNPs 进行了表征。此外,还研究了它们抑制大肠杆菌、金黄色葡萄球菌和白色念珠菌生长和生物膜形成的效果。大肠杆菌和金黄色葡萄球菌的最低抑菌浓度(MIC)均为 32 微克/毫升,而白色念珠菌的最低抑菌浓度为 128 微克/毫升。当 AgNPs 的浓度为 250 微克/毫升时,对大肠杆菌和金黄色葡萄球菌的生物膜抑制率分别为 94% 和 92%。此外,通过抗凋亡、4',6-二脒基-2-苯基吲哚(DAPI)、活性氧(ROS)和吖啶橙/溴化乙锭(AO/EtBr)检测,AgNPs 对人类前列腺癌细胞株 DU145 具有显著的毒性作用。我们还对这些病原体和癌细胞系进行了吸收分析,以初步研究其毒性作用的机制。这些研究结果证实,AgNPs 可以作为一种经济、无毒、环保的资源,用于药用 AgNPs 的绿色合成。此外,这种方法还提供了另一种回收策略,有助于生物副产品的可持续利用。
{"title":"Biogenic silver nanoparticle synthesis using orange peel extract and its multifaceted biomedical application.","authors":"Umme Hani, Fawziah Nasser Kidwan, Lamis Ahmed Albarqi, Saud Abduluziz Al-Qahtani, Ruba Muhammad AlHadi, Haifa Abdullah AlZaid, Nazima Haider, Mohammad Azam Ansari","doi":"10.1007/s00449-024-03031-2","DOIUrl":"10.1007/s00449-024-03031-2","url":null,"abstract":"<p><p>The aim of this study was to employ an agro-industrial byproduct, specifically Citrus sinensis peels, as a reservoir of polyphenols. The natural chemicals present in C. sinensis peels serve as reducing agents in an environmentally benign method for synthesizing silver nanoparticles (AgNPs). This methodology not only provides a more environmentally friendly method for synthesizing nanoparticles but also enhances the value of agricultural waste, emphasizing the sustainable utilization of resources. In our study, AgNPs were successfully synthesized using peel aqueous exact of C. sinensis and then their various biological activity has been investigated. The synthesized AgNPs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and transmission electron microscopy (TEM) analysis. Furthermore, their effectiveness in inhibiting growth and biofilm formation of Escherichia coli, Staphylococcus aureus, and Candida albicans has been investigated. The minimum inhibitory concentrations (MIC) for E. coli and S. aureus were both 32 μg/mL, and for C. albicans, it was 128 µg/mL. At 250 µg/mL of AgNPs, 94% and 92% biofilm inhibition were observed against E. coli and S. aureus, respectively. Furthermore, AgNPs demonstrated significant toxic effects against human prostate cancer cell line DU145 as investigated by anti-apoptotic, 4',6-diamidino-2-phenylindole (DAPI), reactive oxygen species (ROS), and acridine orange/ethidium bromide (AO/EtBr) assays. We also conducted uptake analysis on these pathogens and cancer cell lines to preliminarily investigate the mechanisms underlying their toxic effects. These findings confirm that AgNPs can serve as a cost-effective, non-toxic, and environmentally friendly resource for green synthesis of medicinal AgNPs. Moreover, this approach offers an alternative recycling strategy that contributes to the sustainable use of biological by-products.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Formulation of silver nanoparticles using Duabanga grandiflora leaf extract and evaluation of their versatile therapeutic applications. 使用杜邦叶提取物配制银纳米粒子并评估其多功能治疗应用。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-02-29 DOI: 10.1007/s00449-024-02975-9
Priya Das, Gouhar Jahan Ashraf, Tania Baishya, Tarun Kumar Dua, Paramita Paul, Gouranga Nandi, Ankita Dutta, Divya Limbu, Anoop Kumar, Manab Deb Adhikari, Saikat Dewanjee, Ranabir Sahu

The current research focused on the green synthesis of silver nanoparticles (AgNPs) using Duabanga grandiflora leaf extract. The green synthesis of AgNPs was confirmed by the surface plasmon resonance band at 453 nm in a UV-Visible analysis. The formulated AgNPs had a diameter of around 99.72 nm with a spherical shape. Fourier transform infrared (FTIR) spectrum revealed the bio-reducing potential of phytochemicals present in D. grandiflora, which fundamentally influenced the synthesis of AgNPs. Zeta potential, dynamic light scattering (DLS), scanning electron microscopic (SEM), energy-dispersive X-ray spectroscopic (EDX), X-ray diffraction (XRD), and transmission electron microscopic (TEM) analyses were executed to reveal the physicochemical attributes of the AgNPs. The AgNPs were further investigated for their antioxidant, antidiabetic, anticancer, and antibacterial potential. The DPPH free radical assay revealed the potential radical scavenging capacity (IC50 = 76.73 μg/ml) of green synthesized AgNPs. α-Amylase inhibitory assay displayed significant inhibitory potential (IC50 = 162.11 μg/ml) of this starch-breaking enzyme by AgNPs, revealing the antidiabetic potential of AgNPs. AgNPs exhibited potential cytotoxic activity (IC50 = 244.57 µg/ml) against malignant human kidney cells. In addition, AgNPs showed outstanding antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial strains. Interestingly, AgNPs showed cytotoxic and antimicrobial activities at much higher concentrations than radical scavenging and α-amylase inhibitory concentrations. Thus, our finding elaborated the scope of green synthesized AgNPs for diverse therapeutic applications (dose-dependent) for further clinical translation.

目前的研究重点是利用杜邦叶提取物绿色合成银纳米粒子(AgNPs)。在紫外-可见光分析中,453 nm 处的表面等离子共振波段证实了 AgNPs 的绿色合成。配制的 AgNPs 直径约为 99.72 nm,呈球形。傅立叶变换红外光谱(FTIR)显示了大叶女贞中植物化学物质的生物还原潜力,这从根本上影响了 AgNPs 的合成。Zeta 电位、动态光散射(DLS)、扫描电子显微镜(SEM)、能量色散 X 射线光谱(EDX)、X 射线衍射(XRD)和透射电子显微镜(TEM)分析揭示了 AgNPs 的物理化学属性。实验还进一步研究了 AgNPs 的抗氧化、抗糖尿病、抗癌和抗菌潜力。DPPH 自由基测定显示了绿色合成 AgNPs 的潜在自由基清除能力(IC50 = 76.73 μg/ml)。α-淀粉酶抑制测定显示了 AgNPs 对这种淀粉分解酶的显著抑制潜力(IC50 = 162.11 μg/ml),揭示了 AgNPs 的抗糖尿病潜力。AgNPs 对恶性人类肾脏细胞具有潜在的细胞毒性活性(IC50 = 244.57 µg/ml)。此外,AgNPs 还对革兰氏阴性(大肠杆菌)和革兰氏阳性(金黄色葡萄球菌)细菌菌株表现出卓越的抗菌活性。有趣的是,与自由基清除浓度和 α 淀粉酶抑制浓度相比,AgNPs 的细胞毒性和抗菌活性浓度要高得多。因此,我们的发现阐明了绿色合成的 AgNPs 在不同治疗领域的应用范围(剂量依赖性),可进一步应用于临床。
{"title":"Formulation of silver nanoparticles using Duabanga grandiflora leaf extract and evaluation of their versatile therapeutic applications.","authors":"Priya Das, Gouhar Jahan Ashraf, Tania Baishya, Tarun Kumar Dua, Paramita Paul, Gouranga Nandi, Ankita Dutta, Divya Limbu, Anoop Kumar, Manab Deb Adhikari, Saikat Dewanjee, Ranabir Sahu","doi":"10.1007/s00449-024-02975-9","DOIUrl":"10.1007/s00449-024-02975-9","url":null,"abstract":"<p><p>The current research focused on the green synthesis of silver nanoparticles (AgNPs) using Duabanga grandiflora leaf extract. The green synthesis of AgNPs was confirmed by the surface plasmon resonance band at 453 nm in a UV-Visible analysis. The formulated AgNPs had a diameter of around 99.72 nm with a spherical shape. Fourier transform infrared (FTIR) spectrum revealed the bio-reducing potential of phytochemicals present in D. grandiflora, which fundamentally influenced the synthesis of AgNPs. Zeta potential, dynamic light scattering (DLS), scanning electron microscopic (SEM), energy-dispersive X-ray spectroscopic (EDX), X-ray diffraction (XRD), and transmission electron microscopic (TEM) analyses were executed to reveal the physicochemical attributes of the AgNPs. The AgNPs were further investigated for their antioxidant, antidiabetic, anticancer, and antibacterial potential. The DPPH free radical assay revealed the potential radical scavenging capacity (IC<sub>50</sub> = 76.73 μg/ml) of green synthesized AgNPs. α-Amylase inhibitory assay displayed significant inhibitory potential (IC<sub>50</sub> = 162.11 μg/ml) of this starch-breaking enzyme by AgNPs, revealing the antidiabetic potential of AgNPs. AgNPs exhibited potential cytotoxic activity (IC<sub>50</sub> = 244.57 µg/ml) against malignant human kidney cells. In addition, AgNPs showed outstanding antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial strains. Interestingly, AgNPs showed cytotoxic and antimicrobial activities at much higher concentrations than radical scavenging and α-amylase inhibitory concentrations. Thus, our finding elaborated the scope of green synthesized AgNPs for diverse therapeutic applications (dose-dependent) for further clinical translation.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morinda citrifolia leaf assisted synthesis of ZnO decorated Ag bio-nanocomposites for in-vitro cytotoxicity, antimicrobial and anticancer applications. 海巴戟叶辅助合成氧化锌装饰银生物纳米复合材料,用于体外细胞毒性、抗菌和抗癌应用。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-21 DOI: 10.1007/s00449-024-02995-5
Gopinath Venkatraman, Priyadarshini Sakthi Mohan, Puteri Shafinaz Abdul-Rahman, Faridah Sonsudin, Barathan Muttiah, Abdurahman Hajinur Hirad, Abdullah A Alarfaj, Shifa Wang

This study used Morinda citrifolia leaf (MCL) extract to synthesise Zinc oxide nanoparticles (ZnO NPs) and ZnO decorated silver nanocomposites (ZnO/Ag NCs). The synthesized nanomaterials structural morphology and crystallinity were characterized using a Field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD) analysis. The antimicrobial activity of ZnO NPs and ZnO/Ag NCs was evaluated using human nosocomial bacterial pathogens. The highest antimicrobial activity was recorded for ZnO/Ag NCs at the minimum inhibitory concentration (MIC) at 80 and 100 μg/mL for Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, Staphylococcus aureus than ZnO NPs at the MIC of 120 and 140 μg/mL for Bacillus subtilis and Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. Furthermore, ROS detection, viability assay and bacterial membrane integrity analysis of ZnO/Ag NCs treated P. aeruginosa and S. aureus revealed the fundamental bactericidal mechanism involving cell wall, cell membrane interaction and release of cytoplasmic contents. In addition, ZnO/Ag NCs and ZnO NPs showed higher toxicity towards A549 lung cancer cells than the non-cancerous RAW264 macrophage cells, with IC50 of 242 and 398 µg/mL respectively, compared to IC50 of 402 and 494 µg/mL for the macrophage cells. These results suggest that the ZnO/Ag NCs can be effectively used to develop antimicrobial and anticancer materials.

本研究利用海巴戟叶(MCL)提取物合成了氧化锌纳米颗粒(ZnO NPs)和氧化锌装饰银纳米复合材料(ZnO/Ag NCs)。利用场发射扫描电子显微镜(FESEM)和 X 射线衍射(XRD)分析对合成的纳米材料的结构形态和结晶度进行了表征。利用人体病原菌对氧化锌纳米粒子和氧化锌/银纳米粒子的抗菌活性进行了评估。ZnO/Ag NCs 对大肠杆菌、绿脓杆菌、枯草芽孢杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)分别为 80 和 100 μg/mL,而 ZnO NPs 对枯草芽孢杆菌、大肠杆菌、绿脓杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)分别为 120 和 140 μg/mL,因此 ZnO/Ag NCs 的抗菌活性最高。此外,氧化锌/银数控材料处理铜绿假单胞菌和金黄色葡萄球菌的 ROS 检测、活力测定和细菌膜完整性分析表明,其基本杀菌机制涉及细胞壁、细胞膜相互作用和细胞质内容物的释放。此外,ZnO/Ag NCs 和 ZnO NPs 对 A549 肺癌细胞的毒性高于非癌细胞 RAW264 巨噬细胞,其 IC50 分别为 242 和 398 µg/mL,而巨噬细胞的 IC50 分别为 402 和 494 µg/mL。这些结果表明,ZnO/Ag NCs 可有效用于开发抗菌和抗癌材料。
{"title":"Morinda citrifolia leaf assisted synthesis of ZnO decorated Ag bio-nanocomposites for in-vitro cytotoxicity, antimicrobial and anticancer applications.","authors":"Gopinath Venkatraman, Priyadarshini Sakthi Mohan, Puteri Shafinaz Abdul-Rahman, Faridah Sonsudin, Barathan Muttiah, Abdurahman Hajinur Hirad, Abdullah A Alarfaj, Shifa Wang","doi":"10.1007/s00449-024-02995-5","DOIUrl":"10.1007/s00449-024-02995-5","url":null,"abstract":"<p><p>This study used Morinda citrifolia leaf (MCL) extract to synthesise Zinc oxide nanoparticles (ZnO NPs) and ZnO decorated silver nanocomposites (ZnO/Ag NCs). The synthesized nanomaterials structural morphology and crystallinity were characterized using a Field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD) analysis. The antimicrobial activity of ZnO NPs and ZnO/Ag NCs was evaluated using human nosocomial bacterial pathogens. The highest antimicrobial activity was recorded for ZnO/Ag NCs at the minimum inhibitory concentration (MIC) at 80 and 100 μg/mL for Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, Staphylococcus aureus than ZnO NPs at the MIC of 120 and 140 μg/mL for Bacillus subtilis and Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. Furthermore, ROS detection, viability assay and bacterial membrane integrity analysis of ZnO/Ag NCs treated P. aeruginosa and S. aureus revealed the fundamental bactericidal mechanism involving cell wall, cell membrane interaction and release of cytoplasmic contents. In addition, ZnO/Ag NCs and ZnO NPs showed higher toxicity towards A549 lung cancer cells than the non-cancerous RAW264 macrophage cells, with IC<sub>50</sub> of 242 and 398 µg/mL respectively, compared to IC<sub>50</sub> of 402 and 494 µg/mL for the macrophage cells. These results suggest that the ZnO/Ag NCs can be effectively used to develop antimicrobial and anticancer materials.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140179359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green synthesis of silver and gold nanoparticles in Callistemon viminalis extracts and their antimicrobial activities. 在马蹄莲提取物中绿色合成银和金纳米粒子及其抗菌活性。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-21 DOI: 10.1007/s00449-024-02994-6
Shahid Khan, Abdur Rauf, Abdullah S M Aljohani, Yahya S Al-Awthan, Zubair Ahmad, Omar S Bahattab, Shehla Khan, Muhammad Saadiq, Shahid Ali Khan, Rekha Thiruvengadam, Muthu Thiruvengadam

In the current study, the bottlebrush [Callistemon viminalis (Sol. ex Gaertn.) G. Don] plant was selected for the green synthesis of silver (Ag) and gold (Au) nanoparticles and to evaluate its antibacterial and antifungal activities. Phytochemical screening of C. viminalis confirmed the presence of alkaloids, anthraquinones, saponins, tannins, betacyanins, phlobatanins, coumarins, terpenoids, steroids, glycosides, and proteins. To characterize the synthesized Ag and Au NPs, UV-Visible spectroscopy, FTIR spectroscopy for functional group identification, field emission scanning electron microscopy (FE-SEM) for particle size, and elemental analysis were performed using EDX. The UV-Visible absorption spectra of the green-synthesized Ag and Au nanoparticles were found to have a maximum absorption band at 420 nm for Ag NPs and 525 nm for Au NPs. FE-SEM analysis of the synthesized NPs revealed a circular shape with a size of 100 nm. Elemental analysis was performed for the synthesis of Ag and Au NPs, which confirmed the purity of the nanoparticles. The greenly synthesized Ag and Au NPs were also evaluated for their anti-bacterial and anti-fungal activities, which exhibited prominent inhibition activities against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans, C. krusei, Aspergillus sp., and Trichoderma species. The highest zone of inhibition 15.5 ± 0.75 and 15 ± 0.85 mm was observed for Ag NPs against E. coli and P. aeruginosa. Similarly, Trichoderma sp. and Aspergillus sp. were inhibited by Ag NPs up to 13.5 ± 0.95 and 13 ± 0.70 mm. This work will open doors for the development of new antimicrobial agents using green chemistry.

在本研究中,选择了瓶子草[Callistemon viminalis (Sol. ex Gaertn.) G. Don]植物用于银(Ag)和金(Au)纳米粒子的绿色合成,并评估其抗菌和抗真菌活性。C. viminalis 的植物化学筛选证实了生物碱、蒽醌类、皂苷、单宁酸、贝特矢车菊素、酞檀素、香豆素、萜类、甾体、苷类和蛋白质的存在。为了表征合成的银和金 NPs,我们使用了紫外可见光谱、傅立叶变换红外光谱来鉴定官能团,使用场发射扫描电子显微镜(FE-SEM)来测量粒度,并使用 EDX 进行元素分析。绿色合成的银纳米颗粒和金纳米颗粒的紫外可见吸收光谱显示,银纳米颗粒的最大吸收带在 420 纳米处,金纳米颗粒的最大吸收带在 525 纳米处。对合成的 NPs 进行的 FE-SEM 分析表明,它们呈圆形,大小为 100 nm。对合成的银纳米粒子和金纳米粒子进行了元素分析,证实了纳米粒子的纯度。此外,还对绿色合成的银和金纳米粒子进行了抗菌和抗真菌活性评估,结果表明它们对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、绿脓杆菌、白色念珠菌、克鲁西菌、曲霉菌和毛霉菌具有显著的抑制活性。Ag NPs 对大肠杆菌和铜绿假单胞菌的最大抑菌区分别为 15.5 ± 0.75 毫米和 15 ± 0.85 毫米。同样,Ag NPs 对毛霉菌和曲霉菌的抑制面积分别达到 13.5 ± 0.95 毫米和 13 ± 0.70 毫米。这项工作将为利用绿色化学开发新型抗菌剂打开大门。
{"title":"Green synthesis of silver and gold nanoparticles in Callistemon viminalis extracts and their antimicrobial activities.","authors":"Shahid Khan, Abdur Rauf, Abdullah S M Aljohani, Yahya S Al-Awthan, Zubair Ahmad, Omar S Bahattab, Shehla Khan, Muhammad Saadiq, Shahid Ali Khan, Rekha Thiruvengadam, Muthu Thiruvengadam","doi":"10.1007/s00449-024-02994-6","DOIUrl":"10.1007/s00449-024-02994-6","url":null,"abstract":"<p><p>In the current study, the bottlebrush [Callistemon viminalis (Sol. ex Gaertn.) G. Don] plant was selected for the green synthesis of silver (Ag) and gold (Au) nanoparticles and to evaluate its antibacterial and antifungal activities. Phytochemical screening of C. viminalis confirmed the presence of alkaloids, anthraquinones, saponins, tannins, betacyanins, phlobatanins, coumarins, terpenoids, steroids, glycosides, and proteins. To characterize the synthesized Ag and Au NPs, UV-Visible spectroscopy, FTIR spectroscopy for functional group identification, field emission scanning electron microscopy (FE-SEM) for particle size, and elemental analysis were performed using EDX. The UV-Visible absorption spectra of the green-synthesized Ag and Au nanoparticles were found to have a maximum absorption band at 420 nm for Ag NPs and 525 nm for Au NPs. FE-SEM analysis of the synthesized NPs revealed a circular shape with a size of 100 nm. Elemental analysis was performed for the synthesis of Ag and Au NPs, which confirmed the purity of the nanoparticles. The greenly synthesized Ag and Au NPs were also evaluated for their anti-bacterial and anti-fungal activities, which exhibited prominent inhibition activities against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans, C. krusei, Aspergillus sp., and Trichoderma species. The highest zone of inhibition 15.5 ± 0.75 and 15 ± 0.85 mm was observed for Ag NPs against E. coli and P. aeruginosa. Similarly, Trichoderma sp. and Aspergillus sp. were inhibited by Ag NPs up to 13.5 ± 0.95 and 13 ± 0.70 mm. This work will open doors for the development of new antimicrobial agents using green chemistry.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing nature's potential: Alpinia galanga methanolic extract mediated green synthesis of silver nanoparticle, characterization and evaluation of anti-neoplastic activity. 利用大自然的潜力:高良姜甲醇提取物介导的银纳米粒子的绿色合成、表征和抗肿瘤活性评估。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-21 DOI: 10.1007/s00449-024-02993-7
Ejaj Ahmad, Alina Athar, Nimisha, Qamar Zia, Abhay Kumar Sharma, Mohammed Sajid, Mausumi Bharadwaj, Mairaj Ahmed Ansari, Sundeep Singh Saluja

With the advent of nanotechnology, the treatment of cancer is changing from a conventional to a nanoparticle-based approach. Thus, developing nanoparticles to treat cancer is an area of immense importance. We prepared silver nanoparticles (AgNPs) from methanolic extract of Alpinia galanga rhizome and characterized them by UV-Vis spectrophotometry, Fourier transform Infrared (FTIR) spectroscopy, Zetasizer, and Transmission electron Microscopy (TEM). UV-Vis spectrophotometry absorption spectrum showed surface plasmon between 400 and 480 nm. FTIR spectrum analysis implies that various phytochemicals/secondary metabolites are involved in the reduction, caping, and stabilization of AgNPs. The Zetasier result suggests that the particles formed are small in size with a low polydispersity index (PDI), suggesting a narrow range of particle distribution. The TEM image suggests that the particles formed are mostly of spherical morphology with nearly 20-25 nm. Further, the selected area electron diffraction (SAED) image showed five electron diffraction rings, suggesting the polycrystalline nature of the particles. The nanoparticles showed high anticancer efficacy against cervical cancer (SiHa) cell lines. The nanostructures showed dose-dependent inhibition with 40% killing observed at 6.25 µg/mL dose. The study showed an eco-friendly and cost-effective approach to the synthesis of AgNPs and provided insight into the development of antioxidant and anticancer agents.

随着纳米技术的出现,治疗癌症的方法正在从传统方法转变为基于纳米粒子的方法。因此,开发治疗癌症的纳米粒子是一个极其重要的领域。我们从高良姜根茎的甲醇提取物中制备了银纳米粒子(AgNPs),并通过紫外可见分光光度法、傅立叶变换红外光谱法、Zetasizer 和透射电子显微镜(TEM)对其进行了表征。紫外可见分光光度法的吸收光谱显示出 400 至 480 纳米之间的表面等离子体。傅立叶变换红外光谱分析表明,各种植物化学物质/次生代谢物参与了 AgNPs 的还原、帽化和稳定过程。Zetasier 分析结果表明,所形成的颗粒尺寸较小,多分散指数(PDI)较低,表明颗粒分布范围较窄。TEM 图像表明,形成的颗粒大多呈球形,近 20-25 纳米。此外,选区电子衍射(SAED)图像显示了五个电子衍射环,表明颗粒具有多晶性质。该纳米粒子对宫颈癌(SiHa)细胞株具有很高的抗癌效果。纳米结构显示出剂量依赖性抑制作用,在 6.25 µg/mL 剂量时,可观察到 40% 的杀灭率。该研究展示了一种生态友好、经济高效的 AgNPs 合成方法,并为抗氧化剂和抗癌剂的开发提供了启示。
{"title":"Harnessing nature's potential: Alpinia galanga methanolic extract mediated green synthesis of silver nanoparticle, characterization and evaluation of anti-neoplastic activity.","authors":"Ejaj Ahmad, Alina Athar, Nimisha, Qamar Zia, Abhay Kumar Sharma, Mohammed Sajid, Mausumi Bharadwaj, Mairaj Ahmed Ansari, Sundeep Singh Saluja","doi":"10.1007/s00449-024-02993-7","DOIUrl":"10.1007/s00449-024-02993-7","url":null,"abstract":"<p><p>With the advent of nanotechnology, the treatment of cancer is changing from a conventional to a nanoparticle-based approach. Thus, developing nanoparticles to treat cancer is an area of immense importance. We prepared silver nanoparticles (AgNPs) from methanolic extract of Alpinia galanga rhizome and characterized them by UV-Vis spectrophotometry, Fourier transform Infrared (FTIR) spectroscopy, Zetasizer, and Transmission electron Microscopy (TEM). UV-Vis spectrophotometry absorption spectrum showed surface plasmon between 400 and 480 nm. FTIR spectrum analysis implies that various phytochemicals/secondary metabolites are involved in the reduction, caping, and stabilization of AgNPs. The Zetasier result suggests that the particles formed are small in size with a low polydispersity index (PDI), suggesting a narrow range of particle distribution. The TEM image suggests that the particles formed are mostly of spherical morphology with nearly 20-25 nm. Further, the selected area electron diffraction (SAED) image showed five electron diffraction rings, suggesting the polycrystalline nature of the particles. The nanoparticles showed high anticancer efficacy against cervical cancer (SiHa) cell lines. The nanostructures showed dose-dependent inhibition with 40% killing observed at 6.25 µg/mL dose. The study showed an eco-friendly and cost-effective approach to the synthesis of AgNPs and provided insight into the development of antioxidant and anticancer agents.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140179356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potentials of roots, stems, leaves, flowers, fruits, and seeds extract for the synthesis of silver nanoparticles. 根、茎、叶、花、果实和种子提取物合成银纳米粒子的潜力。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-06-21 DOI: 10.1007/s00449-024-03044-x
Nurul Nazirah Saifuddin, Shaidatul Najihah Matussin, Qazi Fariduddin, Mohammad Mansoob Khan

Silver nanoparticles (AgNPs) have gained significant attention in various applications due to their unique properties that differ from bulk or macro-sized counterparts. In the advancement of nanotechnology, a reliable, non-toxic, and eco-friendly green synthesis has widely been developed as an alternative method for the production of AgNPs, overcoming limitations associated with the traditional physical and chemical methods. Green synthesis of AgNPs involves the utilization of biological sources including plant extracts with silver salt as the precursor. The potential of phytochemicals in plant extracts serves as a reducing/capping and stabilizing agent to aid in the bio-reduction of Ag+ ions into a stable nanoform, Ag0. This review provides insights into the potentials of various plant parts like root, stem, leaf, flower, fruit, and seed extracts that have been extensively reported for the synthesis of AgNPs.

银纳米粒子(AgNPs)因其不同于大块或大尺寸银纳米粒子的独特性质,在各种应用中获得了极大关注。随着纳米技术的发展,一种可靠、无毒、环保的绿色合成方法已被广泛开发出来,作为生产 AgNPs 的替代方法,克服了传统物理和化学方法的局限性。AgNPs 的绿色合成涉及利用生物资源,包括以银盐为前体的植物提取物。植物提取物中潜在的植物化学物质可作为还原/封盖和稳定剂,帮助生物将 Ag+ 离子还原成稳定的纳米形式 Ag0。本综述深入探讨了各种植物部位(如根、茎、叶、花、果实和种子提取物)在合成 AgNPs 方面的潜力,这些植物提取物已被广泛报道。
{"title":"Potentials of roots, stems, leaves, flowers, fruits, and seeds extract for the synthesis of silver nanoparticles.","authors":"Nurul Nazirah Saifuddin, Shaidatul Najihah Matussin, Qazi Fariduddin, Mohammad Mansoob Khan","doi":"10.1007/s00449-024-03044-x","DOIUrl":"10.1007/s00449-024-03044-x","url":null,"abstract":"<p><p>Silver nanoparticles (AgNPs) have gained significant attention in various applications due to their unique properties that differ from bulk or macro-sized counterparts. In the advancement of nanotechnology, a reliable, non-toxic, and eco-friendly green synthesis has widely been developed as an alternative method for the production of AgNPs, overcoming limitations associated with the traditional physical and chemical methods. Green synthesis of AgNPs involves the utilization of biological sources including plant extracts with silver salt as the precursor. The potential of phytochemicals in plant extracts serves as a reducing/capping and stabilizing agent to aid in the bio-reduction of Ag<sup>+</sup> ions into a stable nanoform, Ag<sup>0</sup>. This review provides insights into the potentials of various plant parts like root, stem, leaf, flower, fruit, and seed extracts that have been extensively reported for the synthesis of AgNPs.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bionanocomposites comprising mesoporous metal organic framework (ZIF-8) phytofabricated with Allium sativum as alternative nanomaterials to combat antimicrobial drug resistance. 用薤白植物制造的介孔金属有机框架(ZIF-8)组成的仿生复合材料,可作为对抗抗菌药物耐药性的替代纳米材料。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-20 DOI: 10.1007/s00449-024-03027-y
Suriya Rehman, Ghadi Albhishiri, Zainab Alsalem, Suhailah S AlJameel, Ayman Al-Qaaneh, Aarif Hussain Shah, Sultan Akhtar, Saif Hameed, B Rabindran Jermy

Green nanotechnology is one of the most expanding fields that provides numerous novel nanoparticle drug formulations with enhanced bioactivity performance. This study aims to synthesize mesoporous metal organic framework (ZIF-8) phytofabricated with the herb Allium sativum (As) as an indicator system for its antibacterial and antifungal impact. The successful synthesis of ZIF-8 as nanocomposite was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and scanning coupled with energy-dispersive X-ray spectroscopy and transmission electron microscopy (SEM-EDX and TEM) that showed the textural retainment of ZIF-8 on composite formation with A. sativum. The nanocomposite, A. sativum extract, and ZIF-8 were subjected to antimicrobial assays against Shigella flexneri, Candida albicans, and Candida parapsilosis. The comparative results indicated the potential action of nanocomposite against the bacteria and both the Candida sps; however, the antifungal action against the Candida sps was more effective than the bacterium S. flexneri. The findings suggest that plants, being an important component of ecosystems, could be further explored for the novel drug discovery using green nanotechnology to enhance their impact on the drug-resistant pathogens.

绿色纳米技术是最具扩展性的领域之一,它提供了大量具有增强生物活性性能的新型纳米颗粒药物制剂。本研究旨在以薤白(As)作为抗菌和抗真菌影响的指标体系,合成介孔金属有机框架(ZIF-8)。通过 X 射线衍射 (XRD)、傅立叶变换红外光谱 (FT-IR)、扫描耦合能量色散 X 射线光谱和透射电子显微镜 (SEM-EDX 和 TEM) 对 ZIF-8 纳米复合材料的成功合成进行了表征,结果表明 ZIF-8 与薤白形成复合材料时具有纹理保留。对纳米复合材料、荠菜提取物和 ZIF-8 进行了抗志贺氏菌(Shigella flexneri)、白色念珠菌(Candida albicans)和副白色念珠菌(Candida parapsilosis)的抗菌试验。比较结果表明,纳米复合材料对细菌和白色念珠菌都有潜在作用;但对白色念珠菌的抗真菌作用比对志贺氏菌的作用更有效。研究结果表明,植物是生态系统的重要组成部分,可以利用绿色纳米技术进一步探索新型药物,以增强其对耐药性病原体的作用。
{"title":"Bionanocomposites comprising mesoporous metal organic framework (ZIF-8) phytofabricated with Allium sativum as alternative nanomaterials to combat antimicrobial drug resistance.","authors":"Suriya Rehman, Ghadi Albhishiri, Zainab Alsalem, Suhailah S AlJameel, Ayman Al-Qaaneh, Aarif Hussain Shah, Sultan Akhtar, Saif Hameed, B Rabindran Jermy","doi":"10.1007/s00449-024-03027-y","DOIUrl":"10.1007/s00449-024-03027-y","url":null,"abstract":"<p><p>Green nanotechnology is one of the most expanding fields that provides numerous novel nanoparticle drug formulations with enhanced bioactivity performance. This study aims to synthesize mesoporous metal organic framework (ZIF-8) phytofabricated with the herb Allium sativum (As) as an indicator system for its antibacterial and antifungal impact. The successful synthesis of ZIF-8 as nanocomposite was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and scanning coupled with energy-dispersive X-ray spectroscopy and transmission electron microscopy (SEM-EDX and TEM) that showed the textural retainment of ZIF-8 on composite formation with A. sativum. The nanocomposite, A. sativum extract, and ZIF-8 were subjected to antimicrobial assays against Shigella flexneri, Candida albicans, and Candida parapsilosis. The comparative results indicated the potential action of nanocomposite against the bacteria and both the Candida sps; however, the antifungal action against the Candida sps was more effective than the bacterium S. flexneri. The findings suggest that plants, being an important component of ecosystems, could be further explored for the novel drug discovery using green nanotechnology to enhance their impact on the drug-resistant pathogens.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141064614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phytofabrication of biocompatible zinc oxide nanoparticle using Gymnema sylvestre and its potent in vitro antibacterial, antibiofilm, and cytotoxicity against human breast cancer cells (MDA-MB-231). 利用茜草植物制造生物相容性氧化锌纳米粒子及其对人类乳腺癌细胞(MDA-MB-231)的体外抗菌、抗生物膜和细胞毒性作用。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-31 DOI: 10.1007/s00449-024-03035-y
R Kavipriya, R Ramasubburayan

The increasing incidence of breast cancer and bacterial biofilm in medical devices significantly heightens global mortality and morbidity, challenging synthetic drugs. Consequently, greener-synthesized nanomaterials have emerged as a versatile alternative for various biomedical applications, offering new therapeutic avenues. This study explores the synthesis of biocompatible zinc oxide (ZnONPs) nanoparticles using Gymnema sylvestre and its antibacterial, antibiofilm, and cytotoxic properties. Characterization of ZnONPs inferred that UV-Vis spectra exhibited a sharp peak at 370 nm. Fourier transform infrared spectroscopical analysis revealed the presence of active functional groups such as aldehyde, alkyne, cyclic alkene, sulfate, alkyl aryl ether, and Zn-O bonds. X-ray diffraction analysis results confirmed the crystalline nature of the nanoparticle. Scanning electron microscope analysis evidenced hexagonal morphology, and energy-dispersive X-ray analysis confirmed zinc content. High-resolution transmission electron microscope analysis showed hexagonal and rod-shaped ZnONPs with a size of 5 nm. Zeta potential results affirmed the stability of nanoparticles. The ZnONPs effectively inhibited gram-positive (18-20 mm) than gram-negative (12-18 mm) bacterial pathogens with lower bacteriostatic and higher bactericidal values. Biofilm inhibitory property inferred ZnONPs were more effective against gram-positive (38-94%) than gram-negative bacteria (27-86%). The concentration of ZnONPs to exert 50% biofilm-inhibitory is lower against gram-positive bacteria (179.26-203.95 μg/mL) than gram-negative bacteria (201.46-236.19 μg/mL). Microscopic visualization inferred that at 250 μg/mL, ZnONPs strongly disrupted biofilm formation, as evidenced by decreased biofilm density and altered architecture. The cytotoxicity of ZnONPs against breast cancer cells showed a dose-dependent reduction in cell viability with an IC50 value of 19.4 µg/mL. AO/EB staining indicated early and late apoptotic cell death of breast cancer cells under fluorescence microscopy. The results of hemolytic activity validated the biocompatibility of the ZnONPs. Thus, the unique properties of the green-synthesized ZnONPs suggest their potential as effective drug carriers for targeted delivery in cancer therapy and the treatment of biofilm-related infections.

乳腺癌和医疗器械中细菌生物膜的发病率不断上升,大大提高了全球死亡率和发病率,对合成药物提出了挑战。因此,绿色合成纳米材料已成为各种生物医学应用的多功能替代品,提供了新的治疗途径。本研究探讨了利用爵床科植物合成生物相容性氧化锌(ZnONPs)纳米颗粒及其抗菌、抗生物膜和细胞毒性特性。对 ZnONPs 的表征推断出,紫外可见光谱在 370 纳米处显示出尖锐的峰值。傅立叶变换红外光谱分析显示了活性官能团的存在,如醛、炔、环烯、硫酸盐、烷基芳基醚和 Zn-O 键。X 射线衍射分析结果证实了纳米粒子的结晶性质。扫描电子显微镜分析表明其形态为六角形,能量色散 X 射线分析证实了锌的含量。高分辨率透射电子显微镜分析显示 ZnONPs 呈六角形和棒状,尺寸为 5 纳米。Zeta 电位结果证实了纳米颗粒的稳定性。ZnONPs 可有效抑制革兰氏阳性(18-20 毫米)细菌病原体,抑菌值低于革兰氏阴性(12-18 毫米)细菌病原体,杀菌值高于革兰氏阳性细菌病原体。根据生物膜抑制特性推断,ZnONPs 对革兰氏阳性菌(38-94%)的抑制率高于对革兰氏阴性菌(27-86%)的抑制率。ZnONPs 对革兰氏阳性菌(179.26-203.95 μg/mL)产生 50%生物膜抑制作用的浓度低于革兰氏阴性菌(201.46-236.19 μg/mL)。显微镜观察推断,在 250 μg/mL 的浓度下,ZnONPs 能强烈破坏生物膜的形成,生物膜密度的降低和结构的改变就是证明。ZnONPs 对乳腺癌细胞的细胞毒性显示,细胞存活率的降低呈剂量依赖性,IC50 值为 19.4 µg/mL。在荧光显微镜下,AO/EB 染色显示了乳腺癌细胞的早期和晚期凋亡。溶血活性结果验证了 ZnONPs 的生物相容性。因此,绿色合成的 ZnONPs 的独特性质表明,它们有望成为有效的药物载体,用于癌症治疗和生物膜相关感染的靶向递送。
{"title":"Phytofabrication of biocompatible zinc oxide nanoparticle using Gymnema sylvestre and its potent in vitro antibacterial, antibiofilm, and cytotoxicity against human breast cancer cells (MDA-MB-231).","authors":"R Kavipriya, R Ramasubburayan","doi":"10.1007/s00449-024-03035-y","DOIUrl":"10.1007/s00449-024-03035-y","url":null,"abstract":"<p><p>The increasing incidence of breast cancer and bacterial biofilm in medical devices significantly heightens global mortality and morbidity, challenging synthetic drugs. Consequently, greener-synthesized nanomaterials have emerged as a versatile alternative for various biomedical applications, offering new therapeutic avenues. This study explores the synthesis of biocompatible zinc oxide (ZnONPs) nanoparticles using Gymnema sylvestre and its antibacterial, antibiofilm, and cytotoxic properties. Characterization of ZnONPs inferred that UV-Vis spectra exhibited a sharp peak at 370 nm. Fourier transform infrared spectroscopical analysis revealed the presence of active functional groups such as aldehyde, alkyne, cyclic alkene, sulfate, alkyl aryl ether, and Zn-O bonds. X-ray diffraction analysis results confirmed the crystalline nature of the nanoparticle. Scanning electron microscope analysis evidenced hexagonal morphology, and energy-dispersive X-ray analysis confirmed zinc content. High-resolution transmission electron microscope analysis showed hexagonal and rod-shaped ZnONPs with a size of 5 nm. Zeta potential results affirmed the stability of nanoparticles. The ZnONPs effectively inhibited gram-positive (18-20 mm) than gram-negative (12-18 mm) bacterial pathogens with lower bacteriostatic and higher bactericidal values. Biofilm inhibitory property inferred ZnONPs were more effective against gram-positive (38-94%) than gram-negative bacteria (27-86%). The concentration of ZnONPs to exert 50% biofilm-inhibitory is lower against gram-positive bacteria (179.26-203.95 μg/mL) than gram-negative bacteria (201.46-236.19 μg/mL). Microscopic visualization inferred that at 250 μg/mL, ZnONPs strongly disrupted biofilm formation, as evidenced by decreased biofilm density and altered architecture. The cytotoxicity of ZnONPs against breast cancer cells showed a dose-dependent reduction in cell viability with an IC<sub>50</sub> value of 19.4 µg/mL. AO/EB staining indicated early and late apoptotic cell death of breast cancer cells under fluorescence microscopy. The results of hemolytic activity validated the biocompatibility of the ZnONPs. Thus, the unique properties of the green-synthesized ZnONPs suggest their potential as effective drug carriers for targeted delivery in cancer therapy and the treatment of biofilm-related infections.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141178895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Bioprocess and Biosystems Engineering
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1