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Bioenzyme mediated hydrodistillation (BMHD) for extraction of mint oil from mentha leaves: improvement in yield and menthol content. 用生物酶介导的水蒸馏法(BMHD)从薄荷叶中提取薄荷油:提高产量和薄荷醇含量。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1007/s00449-024-03041-0
Sunidhi Thakiyal, Surekha Bhatia, Charanjeet Kaur, Urmila Gupta Phutela, Mohammed Shafiq Alam, Rohit Sharma

The present study optimized pre-treatment conditions for bioenzyme-mediated hydro-distillation (BMHD) for extraction of mint oil from mentha leaves and the results were compared with those of traditional hydro-distillation (HD) method using response surface methodology. The bio-enzymes produced from moringa leaves had maximum pectinase activity (287.04 µg of sugar/min/ml) followed by xylanase (87.78 µg of sugar/min/ml) while endoglucanase, exoglucanase and amylase activities were comparatively low. The optimized conditions for HD were 69.08 temperature for 173.70 min with water:sample of 10.0. The optimized conditions for enzyme pre-treatment of mentha leaves by BMHD were enzyme concentration of 8%, for a period of 120 min at an incubation period of 40 ℃. The yield (%) and menthol content (%) of the oil at optimized conditions by HD were 1.55 ml/100 g of sample and 56.40% menthol content, respectively, and for BMHD the yield and menthol content (%) of the oil at optimized conditions were 3.69% and 72.80%, respectively. It was found that BMHD leads to a 130% increase in the yield of mint oil and a 10% increase in menthol content as compared to the HD method. No significant difference in physical parameters was observed in mint oil extracted via both methods. Therefore, BMHD is a cost-effective and sustainable approach having an edge over the HD method without compromising the quality and could be a viable approach for commercial purposes.

本研究对生物酶介导的水蒸馏法(BMHD)从薄荷叶中提取薄荷油的预处理条件进行了优化,并采用响应面法将结果与传统的水蒸馏法(HD)进行了比较。从薄荷叶中提取的生物酶具有最高的果胶酶活性(287.04 微克糖/分钟/毫升),其次是木聚糖酶(87.78 微克糖/分钟/毫升),而内切葡聚糖酶、外切葡聚糖酶和淀粉酶活性相对较低。HD 的优化条件为 69.08 摄氏度,173.70 分钟,水与样品的比例为 10.0。用 BMHD 对薄荷叶进行酶预处理的最佳条件是酶浓度为 8%,孵育期为 40 ℃,时间为 120 分钟。在最佳条件下,HD 的产油量(%)和薄荷醇含量(%)分别为 1.55 毫升/100 克样品和 56.40% 的薄荷醇含量;而在最佳条件下,BMHD 的产油量和薄荷醇含量(%)分别为 3.69% 和 72.80%。与 HD 法相比,BMHD 法的薄荷油产量提高了 130%,薄荷醇含量提高了 10%。两种方法提取的薄荷油在物理参数上没有明显差异。因此,BMHD 是一种具有成本效益和可持续发展的方法,与 HD 方法相比,它在不影响质量的情况下具有优势,可以作为商业用途的可行方法。
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引用次数: 0
Rhodococcus erythropolis ATCC 4277 behavior against different metals and its potential use in waste biomining. 红球菌(Rhodococcus erythropolis ATCC 4277)对不同金属的抗性及其在废物生物采矿中的潜在用途。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-06-18 DOI: 10.1007/s00449-024-03048-7
Igor Yannick das Neves Vasconcellos Brandão, Pedro Henrique Barboza de Souza Silva, Tayna Vale Castori, Yasmim Tavares de Souza, Ricardo Gabbay de Souza, Aline Fontana Batista, Sergio Luis Graciano Petroni, Talita Corrêa Nazareth Zanutto, Claudia Barbosa Ladeira de Campos, Danielle Maass

Rhodococcus erythropolis bacterium is known for its remarkable resistance characteristics that can be useful in several biotechnological processes, such as bioremediation. However, there is scarce knowledge concerning the behavior of this strain against different metals. This study sought to investigate the behavior of R. erythropolis ATCC 4277 against the residue of chalcopyrite and e-waste to verify both resistive capacities to the metals present in these residues and their potential use for biomining processes. These tests were carried out in a stirred tank bioreactor for 48 h, at 24ºC, pH 7.0, using a total volume of 2.0 L containing 2.5% (v/v) of a bacterial pre-culture. The pulp density of chalcopyrite was 5% (w/w), and agitation and oxygen flow rates were set to 250 rpm and 1.5 LO2 min-1, respectively. On the other hand, we utilized a waste of computer printed circuit board (WPCB) with a pulp density of 10% (w/w), agitation at 400 rpm, and an oxygen flow rate of 3.0 LO2 min-1. Metal concentration analyses post-fermentation showed that R. erythropolis ATCC 4277 was able to leach about 38% of the Cu present in the chalcopyrite residue (in ~ 24 h), and 49.5% of Fe, 42.3% of Ni, 27.4% of Al, and 15% Cu present in WPCB (in ~ 24 h). In addition, the strain survived well in the environment containing such metals, demonstrating the potential of using this bacterium for waste biomining processes as well as in other processes with these metals.

红球菌(Rhodococcus erythropolis)以其显著的抗性特征而闻名,可用于生物修复等多种生物技术过程。然而,人们对该菌株对不同金属的抗性知之甚少。本研究试图调查红球菌(R. erythropolis ATCC 4277)对黄铜矿和电子垃圾残留物的抗性,以验证其对这些残留物中金属的抗性及其在生物采矿过程中的潜在用途。这些测试是在一个搅拌槽生物反应器中进行的,在 24ºC 和 pH 值为 7.0 的条件下进行了 48 小时,使用的总体积为 2.0 升,其中含有 2.5%(v/v)的细菌预培养物。黄铜矿的矿浆密度为 5%(重量比),搅拌和氧气流速分别设定为 250 rpm 和 1.5 LO2 min-1。另一方面,我们利用了计算机印刷电路板废料(WPCB),其纸浆密度为 10%(重量比),搅拌速度为 400 rpm,氧气流速为 3.0 LO2 min-1。发酵后的金属浓度分析表明,R. erythropolis ATCC 4277 能够浸出黄铜矿残渣中约 38% 的铜(约 24 小时),以及 WPCB 中 49.5% 的铁、42.3% 的镍、27.4% 的铝和 15% 的铜(约 24 小时)。此外,该菌株在含有这些金属的环境中存活良好,这表明该细菌有潜力用于废物生物采矿过程以及含有这些金属的其他过程。
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引用次数: 0
Optimization of dilution rate and mixed carbon feed for continuous production of recombinant plant sucrose:sucrose 1-fructosyltransferase in Komagataella phaffii. 在 Komagataella phaffii 中连续生产重组植物蔗糖:蔗糖 1-果糖基转移酶的稀释率和混合碳饲料的优化。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-06-21 DOI: 10.1007/s00449-024-03045-w
Avijeet S Jaswal, Ravikrishnan Elangovan, Saroj Mishra

The trisaccharide 1-kestose, a major constituent of commercial fructooligosaccharide (FOS) formulations, shows a superior prebiotic effect compared to higher-chain FOS. The plant sucrose:sucrose 1-fructosyltransferases (1-SST) are extensively used for selective synthesis of lower chain FOS. In this study, enhanced recombinant (r) 1-SST production was achieved in Komagataella phaffii (formerly Pichia pastoris) containing three copies of a codon-optimized Festuca arundinacea 1-SST gene. R1-SST production reached 47 U/mL at the shake-flask level after a 96-h methanol induction phase. A chemostat-based strain characterization methodology was adopted to assess the influence of specific growth rate (µ) on cell-specific r1-SST productivity (Qp) and cell-specific oxygen uptake rate (Qo) under two different feeding strategies across dilution rates from 0.02 to 0.05 h-1. The methanol-sorbitol co-feeding strategy significantly reduced Qo by 46 ± 2.4% compared to methanol-only feeding without compromising r1-SST productivity. Based on the data, a dilution rate of 0.025 h-1 was applied for continuous cultivation of recombinant cells to achieve a sustained r1-SST productivity of 5000 ± 64.4 U/L/h for 15 days.

1-Kestose 三糖是商用果寡糖(FOS)配方的主要成分,与高链 FOS 相比,它具有更优越的益生效果。植物蔗糖:蔗糖 1-果糖基转移酶(1-SST)被广泛用于选择性合成低链 FOS。在这项研究中,在含有三个密码子优化的阿伦丁菊 1-SST 基因拷贝的 Komagataella phaffii(原 Pichia pastoris)中实现了重组 1-SST 的强化生产。经过 96 小时的甲醇诱导阶段,R1-SST 的产量在摇瓶水平上达到了 47 U/mL。采用基于恒温箱的菌株表征方法,评估了在 0.02 至 0.05 h-1 两种不同稀释率的喂养策略下,特定生长速率(µ)对细胞特异性 r1-SST 产率(Qp)和细胞特异性氧吸收率(Qo)的影响。在不影响 r1-SST 生产率的情况下,甲醇-山梨醇共喂策略比只喂甲醇的策略显著降低了 Qo(46 ± 2.4%)。根据这些数据,采用 0.025 h-1 的稀释率连续培养重组细胞,可使 r1-SST 生产率持续达到 5000 ± 64.4 U/L/h,持续 15 天。
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引用次数: 0
A novel cost-effective methodology for the screening of nanocellulose producing micro-organisms. 筛选纳米纤维素生产微生物的经济有效的新方法。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-09 DOI: 10.1007/s00449-024-03049-6
Moniya Katyal, Rakshanda Singh, Ritu Mahajan, Anurekha Sharma, Ranjan Gupta, Neeraj K Aggarwal, Anita Yadav

In this paper, the work has been done to develop a cost-effective methodology, for the isolation of the potential producer of bacterial nanocellulose. No report is available in the literature, on the use of gram flour and table sugar for the screening of nanocellulose-producing isolates. Since commercially used, Hestrin-Schramm medium is expensive for the isolation of nanocellulose-producing micro-organisms, the possibility of using gram flour-table sugar medium was investigated in this work. Qualitative screening of micro-organisms was done using cost-effective medium, i.e., gram flour-table sugar medium. Qualitative analysis of various nanocellulose-producing bacteria depicted that cellulose layer production occurred on both HS medium and gram flour-table sugar medium. The yield of nanocellulose was also better on air-liquid surface in case of gram flour-table sugar medium as compared to HS medium. 16S rRNA was used for molecular characterization of bacterial strain and the best nanocellulose producer was identified as Novacetimonas hansenii BMK-3_NC240423 (isolated from rotten banana). FTIR and FE-SEM studies of nanocellulose pellicle produced on HS medium and gram flour-table sugar medium demonstrated equivalent structural, morphological, and chemical properties. The cost of newly designed medium (0.01967 $/L) is nearly 90 times lower than the Hestrin-Schramm medium (1.748 $/L), which makes the screening of nanocellulose producers very cost-effective. A strategy of using gram flour extract-table sugar medium for the screening of nanocellulose-producing micro-organisms is a novel approach, which will drastically reduce the screening associated cost of cellulose-producing micro-organisms and also motivate the researchers/industries for comprehensive screening programme for getting high cellulose-producing microbes.

本文旨在开发一种具有成本效益的方法,用于分离潜在的纳米纤维素细菌生产者。文献中没有关于使用克粉和食糖筛选纳米纤维素生产分离物的报道。由于市售的 Hestrin-Schramm 培养基价格昂贵,无法用于分离纳米纤维素生产微生物,因此本工作研究了使用克面粉-食糖培养基的可能性。使用经济有效的培养基,即克面粉-食糖培养基,对微生物进行了定性筛选。对各种纳米纤维素生产菌的定性分析表明,纤维素层的生产既发生在 HS 培养基上,也发生在克面粉-食糖培养基上。与 HS 培养基相比,克面粉-绵白糖培养基在空气-液体表面的纳米纤维素产量也更高。采用 16S rRNA 对细菌菌株进行分子鉴定,结果表明最好的纳米纤维素生产者是 Novacetimonas hansenii BMK-3_NC240423(从烂香蕉中分离出来)。傅立叶变换红外光谱(FTIR)和傅立叶变换扫描电子显微镜(FE-SEM)对在 HS 培养基和克粉-蔗糖培养基上产生的纳米纤维素胶粒进行了研究,结果表明其结构、形态和化学特性相当。新设计的培养基成本(0.01967 美元/升)比 Hestrin-Schramm 培养基(1.748 美元/升)低近 90 倍,使纳米纤维素生产者的筛选非常经济有效。使用克面粉提取物-表糖培养基来筛选纳米纤维素生产微生物的策略是一种新方法,它将大大降低纤维素生产微生物的筛选相关成本,同时也激励研究人员/工业界开展全面的筛选计划,以获得高纤维素生产微生物。
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引用次数: 0
Green synthesis of copper nanoparticles by using pineapple peel waste: in vitro characterizations and antibacterial potential. 利用菠萝皮废弃物绿色合成纳米铜粒子:体外表征和抗菌潜力。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-27 DOI: 10.1007/s00449-024-02982-w
Sanchita Mitra, Tarun Kumar Dua, Serina Easmin, Sucheta Sarkar, Arijit Prosad Roy, Ranabir Sahu, Gouranga Nandi, Md Salman Haydar, Swarnendu Roy, Paramita Paul

A considerable amount of fruit waste is being produced every day worldwide. The green synthesis of metal nanoparticles from fruit peel waste can be an innovative, cost-effective, and eco-friendly alternative to traditional methods. Copper nanoparticles (CuNPs) were synthesized by a green method using the pineapple peels extract (PLX) and copper sulfate pentahydrate. The formation of CuNPs was visually identified and detected by UV-Visible spectroscopy. The CuNPs were characterized by Fourier-transform infrared (FTIR) spectroscopy, particle size analyzer, scanning electron microscopy (SEM), and X-ray diffraction (XRD). The antioxidant and reducing power of CuNPs were conducted by %DPPH scavenging and electron transfer-based ferric reducing antioxidant power (FRAP) assay, respectively. The antibacterial properties of CuNPs were determined in gram-positive, and gram-negative bacteria. The results showed that the CuNPs were spherical in shape with mean particle size 290.5 nm. The zeta potential of the nanoparticles was found to be - 12.3 mV indicating the instability in the colloidal state. The FTIR study confirmed the peaks of phytochemicals present in the PLX and the nanoparticles supporting the use of pineapple peels as stabilizing, reducing and capping agents. Both the DPPH and reducing power assay depicted that the synthesized CuNPs had significant antioxidant activity. However, the synthesized CuNPs had strong inhibitory capacity against both gram-positive and gram-negative test organisms. Thus, the CuNPS could be used for its viable antibacterial potential to preserve fruits, flowers, and vegetables from bacterial contamination.

全世界每天都会产生大量的水果废料。利用果皮废弃物绿色合成金属纳米粒子是一种创新、经济、环保的方法,可替代传统方法。本研究采用菠萝果皮提取物(PLX)和五水硫酸铜,以绿色方法合成了纳米铜粒子(CuNPs)。CuNPs 的形成可通过肉眼识别,并可通过紫外可见光谱检测。傅立叶变换红外光谱(FTIR)、粒度分析仪、扫描电子显微镜(SEM)和 X 射线衍射(XRD)对 CuNPs 进行了表征。CuNPs 的抗氧化性和还原力分别通过%DPPH 清除率和基于电子传递的铁还原抗氧化力(FRAP)测定法进行了检测。还测定了 CuNPs 在革兰氏阳性菌和革兰氏阴性菌中的抗菌特性。结果表明,CuNPs 呈球形,平均粒径为 290.5 nm。纳米颗粒的 zeta 电位为 - 12.3 mV,表明其胶体状态不稳定。傅立叶变换红外光谱(FTIR)研究证实了 PLX 和纳米颗粒中存在的植物化学物质峰,支持使用菠萝皮作为稳定剂、还原剂和封盖剂。DPPH 和还原力分析表明,合成的 CuNPs 具有显著的抗氧化活性。不过,合成的 CuNPs 对革兰氏阳性和阴性试验生物都有很强的抑制能力。因此,CuNPS 具有可行的抗菌潜力,可用于保存水果、鲜花和蔬菜,使其免受细菌污染。
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引用次数: 0
Phyto-fabricated ZnO nanoparticles for anticancer, photo-antimicrobial effect on carbapenem-resistant/sensitive Pseudomonas aeruginosa and removal of tetracycline. 用于抗癌、对碳青霉烯耐药/敏感铜绿假单胞菌的光抗菌作用以及去除四环素的植物制备氧化锌纳米粒子。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-16 DOI: 10.1007/s00449-024-02984-8
Gopinath Venkatraman, Priyadarshini Sakthi Mohan, Maryam Mohammed Mashghan, Kar-Cheng Wong, Puteri Shafinaz Abdul-Rahman, Kumutha Malar Vellasamy, Abdurahman Hajinur Hirad, Abdullah A Alarfaj, Shifa Wang

Alternanthera sessilis (AS) leaf extract was used to synthesize zinc oxide nanoparticles (ZnO NPs). Bioanalytical characterization techniques such as X-ray diffraction (XRD) and field emission scanning electron microscope (FESEM) confirmed the formation of crystalline ZnO NPs with average sizes of 40 nm. The AS-ZnO NPs antimicrobial activity was analyzed under dark (D) and white light (WL) conditions. The improved antimicrobial activity was observed against Escherichia coli, Staphylococcus aureus and Bacillus subtilis at the minimal inhibitory concentration (MIC) of 125 and 62.5 µg/mL under WL than the D at 125 and 250 µg/mL for E. coli, B. subtilis, and Pseudomonas aeruginosa, respectively. In contrast, the growth of P. aeruginosa and S. aureus was not completely inhibited until 1 mg/mL AS-ZnO NPs under WL and D. Similarly, AS-ZnO NPs displayed a weaker inhibitory effect against carbapenem-sensitive P. aeruginosa (CSPA) and carbapenem-resistant P. aeruginosa (CRPA) strains of PAC023, PAC041 and PAC032, PAC045 under D. Interestingly, the distinct inhibitory effect was recorded against CSPA PAC041 and CRPA PAC032 in which the bacteria growth was inhibited 99.9% at 250, 500 µg/mL under WL. The cytotoxicity results suggested AS-ZnO NPs demonstrated higher toxicity to MCF-7 breast cancer cells than the RAW264.7 macrophage cells. Further, AS-ZnO NPs exhibited higher catalytic potential against tetracycline hydrochloride (TC-H) degradation at 65.6% and 60.8% under WL than the dark at 59.35% and 48.6% within 120 min. Therefore, AS-ZnO NPs can be used to design a photo-improved antimicrobial formulation and environmental catalyst for removing TC-H from wastewater.

Alternanthera sessilis(AS)叶提取物被用来合成氧化锌纳米粒子(ZnO NPs)。X 射线衍射(XRD)和场发射扫描电子显微镜(FESEM)等生物分析表征技术证实了平均尺寸为 40 nm 的结晶 ZnO NPs 的形成。在黑暗(D)和白光(WL)条件下分析了 AS-ZnO NPs 的抗菌活性。对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的最小抑菌浓度(MIC)分别为 125 微克/毫升和 62.5 微克/毫升时,在 WL 条件下,AS-ZnO NPs 的抗菌活性比在 D 条件下的 125 微克/毫升和 250 微克/毫升有所提高。同样,AS-ZnO NPs 对碳青霉烯敏感的铜绿假单胞菌(CSPA)和碳青霉烯耐药的铜绿假单胞菌(CRPA)的抑制作用也较弱。有趣的是,As-ZnO NPs 对碳青霉烯敏感的铜绿假单胞菌(CSPA)和碳青霉烯耐药的铜绿假单胞菌(CRPA)菌株 PAC023、PAC041 和 PAC032、PAC045 有明显的抑制作用。细胞毒性结果表明,AS-ZnO NPs 对 MCF-7 乳腺癌细胞的毒性高于 RAW264.7 巨噬细胞。此外,在 120 分钟内,AS-ZnO NPs 在 WL 下对盐酸四环素(TC-H)降解的催化潜力分别为 65.6% 和 60.8%,高于黑暗条件下的 59.35% 和 48.6%。因此,AS-ZnO NPs 可用于设计一种光改进的抗菌配方和环境催化剂,以去除废水中的 TC-H。
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引用次数: 0
Biogenic silver nanoparticle synthesis using orange peel extract and its multifaceted biomedical application. 利用橙皮提取物合成生物银纳米粒子及其多方面的生物医学应用。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1007/s00449-024-03031-2
Umme Hani, Fawziah Nasser Kidwan, Lamis Ahmed Albarqi, Saud Abduluziz Al-Qahtani, Ruba Muhammad AlHadi, Haifa Abdullah AlZaid, Nazima Haider, Mohammad Azam Ansari

The aim of this study was to employ an agro-industrial byproduct, specifically Citrus sinensis peels, as a reservoir of polyphenols. The natural chemicals present in C. sinensis peels serve as reducing agents in an environmentally benign method for synthesizing silver nanoparticles (AgNPs). This methodology not only provides a more environmentally friendly method for synthesizing nanoparticles but also enhances the value of agricultural waste, emphasizing the sustainable utilization of resources. In our study, AgNPs were successfully synthesized using peel aqueous exact of C. sinensis and then their various biological activity has been investigated. The synthesized AgNPs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and transmission electron microscopy (TEM) analysis. Furthermore, their effectiveness in inhibiting growth and biofilm formation of Escherichia coli, Staphylococcus aureus, and Candida albicans has been investigated. The minimum inhibitory concentrations (MIC) for E. coli and S. aureus were both 32 μg/mL, and for C. albicans, it was 128 µg/mL. At 250 µg/mL of AgNPs, 94% and 92% biofilm inhibition were observed against E. coli and S. aureus, respectively. Furthermore, AgNPs demonstrated significant toxic effects against human prostate cancer cell line DU145 as investigated by anti-apoptotic, 4',6-diamidino-2-phenylindole (DAPI), reactive oxygen species (ROS), and acridine orange/ethidium bromide (AO/EtBr) assays. We also conducted uptake analysis on these pathogens and cancer cell lines to preliminarily investigate the mechanisms underlying their toxic effects. These findings confirm that AgNPs can serve as a cost-effective, non-toxic, and environmentally friendly resource for green synthesis of medicinal AgNPs. Moreover, this approach offers an alternative recycling strategy that contributes to the sustainable use of biological by-products.

本研究的目的是利用一种农用工业副产品,特别是柑橘皮,作为多酚的宝库。柑橘皮中的天然化学物质可作为还原剂,以一种对环境无害的方法合成银纳米粒子(AgNPs)。这种方法不仅提供了一种更环保的合成纳米粒子的方法,还提高了农业废弃物的价值,强调了资源的可持续利用。在我们的研究中,我们成功地利用去皮水溶液合成了 AgNPs,并对其各种生物活性进行了研究。通过紫外-可见光谱、动态光散射(DLS)、扫描电子显微镜(SEM)、能量色散 X 射线(EDX)和透射电子显微镜(TEM)分析,对合成的 AgNPs 进行了表征。此外,还研究了它们抑制大肠杆菌、金黄色葡萄球菌和白色念珠菌生长和生物膜形成的效果。大肠杆菌和金黄色葡萄球菌的最低抑菌浓度(MIC)均为 32 微克/毫升,而白色念珠菌的最低抑菌浓度为 128 微克/毫升。当 AgNPs 的浓度为 250 微克/毫升时,对大肠杆菌和金黄色葡萄球菌的生物膜抑制率分别为 94% 和 92%。此外,通过抗凋亡、4',6-二脒基-2-苯基吲哚(DAPI)、活性氧(ROS)和吖啶橙/溴化乙锭(AO/EtBr)检测,AgNPs 对人类前列腺癌细胞株 DU145 具有显著的毒性作用。我们还对这些病原体和癌细胞系进行了吸收分析,以初步研究其毒性作用的机制。这些研究结果证实,AgNPs 可以作为一种经济、无毒、环保的资源,用于药用 AgNPs 的绿色合成。此外,这种方法还提供了另一种回收策略,有助于生物副产品的可持续利用。
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引用次数: 0
Morinda citrifolia leaf assisted synthesis of ZnO decorated Ag bio-nanocomposites for in-vitro cytotoxicity, antimicrobial and anticancer applications. 海巴戟叶辅助合成氧化锌装饰银生物纳米复合材料,用于体外细胞毒性、抗菌和抗癌应用。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-21 DOI: 10.1007/s00449-024-02995-5
Gopinath Venkatraman, Priyadarshini Sakthi Mohan, Puteri Shafinaz Abdul-Rahman, Faridah Sonsudin, Barathan Muttiah, Abdurahman Hajinur Hirad, Abdullah A Alarfaj, Shifa Wang

This study used Morinda citrifolia leaf (MCL) extract to synthesise Zinc oxide nanoparticles (ZnO NPs) and ZnO decorated silver nanocomposites (ZnO/Ag NCs). The synthesized nanomaterials structural morphology and crystallinity were characterized using a Field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD) analysis. The antimicrobial activity of ZnO NPs and ZnO/Ag NCs was evaluated using human nosocomial bacterial pathogens. The highest antimicrobial activity was recorded for ZnO/Ag NCs at the minimum inhibitory concentration (MIC) at 80 and 100 μg/mL for Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, Staphylococcus aureus than ZnO NPs at the MIC of 120 and 140 μg/mL for Bacillus subtilis and Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. Furthermore, ROS detection, viability assay and bacterial membrane integrity analysis of ZnO/Ag NCs treated P. aeruginosa and S. aureus revealed the fundamental bactericidal mechanism involving cell wall, cell membrane interaction and release of cytoplasmic contents. In addition, ZnO/Ag NCs and ZnO NPs showed higher toxicity towards A549 lung cancer cells than the non-cancerous RAW264 macrophage cells, with IC50 of 242 and 398 µg/mL respectively, compared to IC50 of 402 and 494 µg/mL for the macrophage cells. These results suggest that the ZnO/Ag NCs can be effectively used to develop antimicrobial and anticancer materials.

本研究利用海巴戟叶(MCL)提取物合成了氧化锌纳米颗粒(ZnO NPs)和氧化锌装饰银纳米复合材料(ZnO/Ag NCs)。利用场发射扫描电子显微镜(FESEM)和 X 射线衍射(XRD)分析对合成的纳米材料的结构形态和结晶度进行了表征。利用人体病原菌对氧化锌纳米粒子和氧化锌/银纳米粒子的抗菌活性进行了评估。ZnO/Ag NCs 对大肠杆菌、绿脓杆菌、枯草芽孢杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)分别为 80 和 100 μg/mL,而 ZnO NPs 对枯草芽孢杆菌、大肠杆菌、绿脓杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)分别为 120 和 140 μg/mL,因此 ZnO/Ag NCs 的抗菌活性最高。此外,氧化锌/银数控材料处理铜绿假单胞菌和金黄色葡萄球菌的 ROS 检测、活力测定和细菌膜完整性分析表明,其基本杀菌机制涉及细胞壁、细胞膜相互作用和细胞质内容物的释放。此外,ZnO/Ag NCs 和 ZnO NPs 对 A549 肺癌细胞的毒性高于非癌细胞 RAW264 巨噬细胞,其 IC50 分别为 242 和 398 µg/mL,而巨噬细胞的 IC50 分别为 402 和 494 µg/mL。这些结果表明,ZnO/Ag NCs 可有效用于开发抗菌和抗癌材料。
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引用次数: 0
Green synthesis of silver and gold nanoparticles in Callistemon viminalis extracts and their antimicrobial activities. 在马蹄莲提取物中绿色合成银和金纳米粒子及其抗菌活性。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-03-21 DOI: 10.1007/s00449-024-02994-6
Shahid Khan, Abdur Rauf, Abdullah S M Aljohani, Yahya S Al-Awthan, Zubair Ahmad, Omar S Bahattab, Shehla Khan, Muhammad Saadiq, Shahid Ali Khan, Rekha Thiruvengadam, Muthu Thiruvengadam

In the current study, the bottlebrush [Callistemon viminalis (Sol. ex Gaertn.) G. Don] plant was selected for the green synthesis of silver (Ag) and gold (Au) nanoparticles and to evaluate its antibacterial and antifungal activities. Phytochemical screening of C. viminalis confirmed the presence of alkaloids, anthraquinones, saponins, tannins, betacyanins, phlobatanins, coumarins, terpenoids, steroids, glycosides, and proteins. To characterize the synthesized Ag and Au NPs, UV-Visible spectroscopy, FTIR spectroscopy for functional group identification, field emission scanning electron microscopy (FE-SEM) for particle size, and elemental analysis were performed using EDX. The UV-Visible absorption spectra of the green-synthesized Ag and Au nanoparticles were found to have a maximum absorption band at 420 nm for Ag NPs and 525 nm for Au NPs. FE-SEM analysis of the synthesized NPs revealed a circular shape with a size of 100 nm. Elemental analysis was performed for the synthesis of Ag and Au NPs, which confirmed the purity of the nanoparticles. The greenly synthesized Ag and Au NPs were also evaluated for their anti-bacterial and anti-fungal activities, which exhibited prominent inhibition activities against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans, C. krusei, Aspergillus sp., and Trichoderma species. The highest zone of inhibition 15.5 ± 0.75 and 15 ± 0.85 mm was observed for Ag NPs against E. coli and P. aeruginosa. Similarly, Trichoderma sp. and Aspergillus sp. were inhibited by Ag NPs up to 13.5 ± 0.95 and 13 ± 0.70 mm. This work will open doors for the development of new antimicrobial agents using green chemistry.

在本研究中,选择了瓶子草[Callistemon viminalis (Sol. ex Gaertn.) G. Don]植物用于银(Ag)和金(Au)纳米粒子的绿色合成,并评估其抗菌和抗真菌活性。C. viminalis 的植物化学筛选证实了生物碱、蒽醌类、皂苷、单宁酸、贝特矢车菊素、酞檀素、香豆素、萜类、甾体、苷类和蛋白质的存在。为了表征合成的银和金 NPs,我们使用了紫外可见光谱、傅立叶变换红外光谱来鉴定官能团,使用场发射扫描电子显微镜(FE-SEM)来测量粒度,并使用 EDX 进行元素分析。绿色合成的银纳米颗粒和金纳米颗粒的紫外可见吸收光谱显示,银纳米颗粒的最大吸收带在 420 纳米处,金纳米颗粒的最大吸收带在 525 纳米处。对合成的 NPs 进行的 FE-SEM 分析表明,它们呈圆形,大小为 100 nm。对合成的银纳米粒子和金纳米粒子进行了元素分析,证实了纳米粒子的纯度。此外,还对绿色合成的银和金纳米粒子进行了抗菌和抗真菌活性评估,结果表明它们对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、绿脓杆菌、白色念珠菌、克鲁西菌、曲霉菌和毛霉菌具有显著的抑制活性。Ag NPs 对大肠杆菌和铜绿假单胞菌的最大抑菌区分别为 15.5 ± 0.75 毫米和 15 ± 0.85 毫米。同样,Ag NPs 对毛霉菌和曲霉菌的抑制面积分别达到 13.5 ± 0.95 毫米和 13 ± 0.70 毫米。这项工作将为利用绿色化学开发新型抗菌剂打开大门。
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引用次数: 0
Formulation of silver nanoparticles using Duabanga grandiflora leaf extract and evaluation of their versatile therapeutic applications. 使用杜邦叶提取物配制银纳米粒子并评估其多功能治疗应用。
IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-02-29 DOI: 10.1007/s00449-024-02975-9
Priya Das, Gouhar Jahan Ashraf, Tania Baishya, Tarun Kumar Dua, Paramita Paul, Gouranga Nandi, Ankita Dutta, Divya Limbu, Anoop Kumar, Manab Deb Adhikari, Saikat Dewanjee, Ranabir Sahu

The current research focused on the green synthesis of silver nanoparticles (AgNPs) using Duabanga grandiflora leaf extract. The green synthesis of AgNPs was confirmed by the surface plasmon resonance band at 453 nm in a UV-Visible analysis. The formulated AgNPs had a diameter of around 99.72 nm with a spherical shape. Fourier transform infrared (FTIR) spectrum revealed the bio-reducing potential of phytochemicals present in D. grandiflora, which fundamentally influenced the synthesis of AgNPs. Zeta potential, dynamic light scattering (DLS), scanning electron microscopic (SEM), energy-dispersive X-ray spectroscopic (EDX), X-ray diffraction (XRD), and transmission electron microscopic (TEM) analyses were executed to reveal the physicochemical attributes of the AgNPs. The AgNPs were further investigated for their antioxidant, antidiabetic, anticancer, and antibacterial potential. The DPPH free radical assay revealed the potential radical scavenging capacity (IC50 = 76.73 μg/ml) of green synthesized AgNPs. α-Amylase inhibitory assay displayed significant inhibitory potential (IC50 = 162.11 μg/ml) of this starch-breaking enzyme by AgNPs, revealing the antidiabetic potential of AgNPs. AgNPs exhibited potential cytotoxic activity (IC50 = 244.57 µg/ml) against malignant human kidney cells. In addition, AgNPs showed outstanding antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial strains. Interestingly, AgNPs showed cytotoxic and antimicrobial activities at much higher concentrations than radical scavenging and α-amylase inhibitory concentrations. Thus, our finding elaborated the scope of green synthesized AgNPs for diverse therapeutic applications (dose-dependent) for further clinical translation.

目前的研究重点是利用杜邦叶提取物绿色合成银纳米粒子(AgNPs)。在紫外-可见光分析中,453 nm 处的表面等离子共振波段证实了 AgNPs 的绿色合成。配制的 AgNPs 直径约为 99.72 nm,呈球形。傅立叶变换红外光谱(FTIR)显示了大叶女贞中植物化学物质的生物还原潜力,这从根本上影响了 AgNPs 的合成。Zeta 电位、动态光散射(DLS)、扫描电子显微镜(SEM)、能量色散 X 射线光谱(EDX)、X 射线衍射(XRD)和透射电子显微镜(TEM)分析揭示了 AgNPs 的物理化学属性。实验还进一步研究了 AgNPs 的抗氧化、抗糖尿病、抗癌和抗菌潜力。DPPH 自由基测定显示了绿色合成 AgNPs 的潜在自由基清除能力(IC50 = 76.73 μg/ml)。α-淀粉酶抑制测定显示了 AgNPs 对这种淀粉分解酶的显著抑制潜力(IC50 = 162.11 μg/ml),揭示了 AgNPs 的抗糖尿病潜力。AgNPs 对恶性人类肾脏细胞具有潜在的细胞毒性活性(IC50 = 244.57 µg/ml)。此外,AgNPs 还对革兰氏阴性(大肠杆菌)和革兰氏阳性(金黄色葡萄球菌)细菌菌株表现出卓越的抗菌活性。有趣的是,与自由基清除浓度和 α 淀粉酶抑制浓度相比,AgNPs 的细胞毒性和抗菌活性浓度要高得多。因此,我们的发现阐明了绿色合成的 AgNPs 在不同治疗领域的应用范围(剂量依赖性),可进一步应用于临床。
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引用次数: 0
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Bioprocess and Biosystems Engineering
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