The ability of silica aerogel nanoparticles (SA-Np) to improve the stability of hydroxyapatite (HA) was investigated. Using the sol-gel method, the HA was incorporated into SA-Np at a weight ratio of 0.5 of HA to SiO2 (HA-SA-Np). The efficacy of HA-SA-Np, SA-Np, and HA on the in vitro migration of normal human dermal fibroblast cells (HSF1184) was compared. The cell migration was measured at 0, 6, and 24 hours after scratching using ImageJ and an inverted optical microscope. To ascertain the resorbability of HA-SA-Np, the phosphate and silicic acid concentrations in media treated for 2, 5, and 7 days were examined. The high dissolution of HA could be reduced by incorporating the HA into the silica nanosphere. The HA-SA-Np significantly stimulated cell migration and increased closure with increasing treatment time. It was caused by the release of silicic acid, which aided in healing cells. It also demonstrates the ability of HA-SA-Np to be resorbed and eventually increase the adhesion and migration of normal human fibroblast cells. As a result, the potential application of HA-SA-Np as an alternative biomaterial was confirmed.
研究了二氧化硅气凝胶纳米粒子（SA Np）改善羟基磷灰石（HA）稳定性的能力。使用溶胶-凝胶法，将HA以HA与SiO2（HA-SA-Np）的0.5的重量比掺入SA-Np中。比较了HA、SA和HA对正常人真皮成纤维细胞（HSF1184）体外迁移的影响。使用ImageJ和倒置光学显微镜在刮擦后0、6和24小时测量细胞迁移。为了确定HA-SA-Np的可吸收性，检测处理2、5和7天的培养基中的磷酸盐和硅酸浓度。通过将HA掺入二氧化硅纳米球中，可以降低HA的高溶解性。HA SA Np显著刺激细胞迁移，并随着处理时间的增加而增加闭合。它是由硅酸的释放引起的，硅酸有助于细胞的愈合。它还证明了HA-SA-Np被再吸收并最终增加正常人成纤维细胞的粘附和迁移的能力。因此，HA-SA-Np作为一种替代生物材料的潜在应用得到了证实。
{"title":"Hydroxyapatite Modified Silica Aerogel Nanoparticles: In Vitro Cell Migration Analysis","authors":"","doi":"10.33263/briac134.373","DOIUrl":"https://doi.org/10.33263/briac134.373","url":null,"abstract":"The ability of silica aerogel nanoparticles (SA-Np) to improve the stability of hydroxyapatite (HA) was investigated. Using the sol-gel method, the HA was incorporated into SA-Np at a weight ratio of 0.5 of HA to SiO2 (HA-SA-Np). The efficacy of HA-SA-Np, SA-Np, and HA on the in vitro migration of normal human dermal fibroblast cells (HSF1184) was compared. The cell migration was measured at 0, 6, and 24 hours after scratching using ImageJ and an inverted optical microscope. To ascertain the resorbability of HA-SA-Np, the phosphate and silicic acid concentrations in media treated for 2, 5, and 7 days were examined. The high dissolution of HA could be reduced by incorporating the HA into the silica nanosphere. The HA-SA-Np significantly stimulated cell migration and increased closure with increasing treatment time. It was caused by the release of silicic acid, which aided in healing cells. It also demonstrates the ability of HA-SA-Np to be resorbed and eventually increase the adhesion and migration of normal human fibroblast cells. As a result, the potential application of HA-SA-Np as an alternative biomaterial was confirmed.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49531849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oral streptococci are the oral microbial flora that can cause biofilm formation. One of the most common isolated oral streptococci is Streptococcus mutans, which has a significant role in oral diseases, including periodontal. The most important factor of S. mutans pathogenesis includes biofilm formation that leads to emptying tooth enamel and caries. Various genes including atpF, gtfB, gtfC, gtfD, gtf, LuxS, comAB, comCDE, and comX regulate biofilm formation. Therefore, in this review, we aimed to investigate factors that influence S. mutans pathogenicity in the mouth. The main factors are related to the biofilm formation of this bacteria and metabolic products, which influence environmental changes by carbohydrate metabolism and help this pathogen to make dominant growth compared to other bacteria living in the oral cavity. Indeed, developing methods to inhibit biofilm formation and quorum sensing using antimicrobial agents with anti-biofilm and antibacterial properties should be considered based on our knowledge of the pathogenicity mechanisms of S.mutans.
{"title":"Factors Associated with Streptococcus mutans Pathogenicity in the Oral Cavity","authors":"","doi":"10.33263/briac134.368","DOIUrl":"https://doi.org/10.33263/briac134.368","url":null,"abstract":"Oral streptococci are the oral microbial flora that can cause biofilm formation. One of the most common isolated oral streptococci is Streptococcus mutans, which has a significant role in oral diseases, including periodontal. The most important factor of S. mutans pathogenesis includes biofilm formation that leads to emptying tooth enamel and caries. Various genes including atpF, gtfB, gtfC, gtfD, gtf, LuxS, comAB, comCDE, and comX regulate biofilm formation. Therefore, in this review, we aimed to investigate factors that influence S. mutans pathogenicity in the mouth. The main factors are related to the biofilm formation of this bacteria and metabolic products, which influence environmental changes by carbohydrate metabolism and help this pathogen to make dominant growth compared to other bacteria living in the oral cavity. Indeed, developing methods to inhibit biofilm formation and quorum sensing using antimicrobial agents with anti-biofilm and antibacterial properties should be considered based on our knowledge of the pathogenicity mechanisms of S.mutans.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43628947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A series of novel thiazolidine-4-one substituted thiazoles were prepared using multi-step synthesis and screened for their antiepileptic potency. The chemical nature of the prepared derivatives was confirmed using FT-IR, elemental analyses, Mass spectroscopy, and 1H-NMR. Molecular properties and antiepileptic potency of the novel thiazoles were predicted using in-silico models such as molinspiration online tool and molecular docking, respectively. In-vivo antiepileptic potency of the entire title compounds was determined using MES and scPTZ method. Additionally, the rotorod test was employed to determine the neurotoxicity of the synthesized derivatives. Entire title analogs displayed -varying degrees of antiepileptic and neurotoxicity potency. The pharmacological potency of tested compounds was compared with their chemical structures. 2-(4-Nitrophenyl)-3-(4-((4-phenylthiazol-2-ylimino)methyl)phenyl) thiazolidin-4-one (PTT6) was found to be the very active derivative of this series among thirteen tested derivatives. Thus, this analog may act as a lead molecule to find potent and safer antiepileptic drugs.
{"title":"Design, In-Silico Studies, Synthesis, Characterization, and Anticonvulsant Activities of Novel Thiazolidin-4-One Substituted Thiazole Derivatives","authors":"","doi":"10.33263/briac134.366","DOIUrl":"https://doi.org/10.33263/briac134.366","url":null,"abstract":"A series of novel thiazolidine-4-one substituted thiazoles were prepared using multi-step synthesis and screened for their antiepileptic potency. The chemical nature of the prepared derivatives was confirmed using FT-IR, elemental analyses, Mass spectroscopy, and 1H-NMR. Molecular properties and antiepileptic potency of the novel thiazoles were predicted using in-silico models such as molinspiration online tool and molecular docking, respectively. In-vivo antiepileptic potency of the entire title compounds was determined using MES and scPTZ method. Additionally, the rotorod test was employed to determine the neurotoxicity of the synthesized derivatives. Entire title analogs displayed -varying degrees of antiepileptic and neurotoxicity potency. The pharmacological potency of tested compounds was compared with their chemical structures. 2-(4-Nitrophenyl)-3-(4-((4-phenylthiazol-2-ylimino)methyl)phenyl) thiazolidin-4-one (PTT6) was found to be the very active derivative of this series among thirteen tested derivatives. Thus, this analog may act as a lead molecule to find potent and safer antiepileptic drugs.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45278901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The barium hexaferrite magnetic materials Mn-doped BaZn1+x Mnx Fe12-2x O19 (x = 0.4, 0.8, 1.2, 1.6, and 2) were prepared using the ceramic method. The powder samples ferrite were subjected to XRD, FT-IR, and UV-Vis to investigate the nanoparticles' structural changes. UV–Vis spectroscopy is used to obtain the samples' absorption spectrum and calculate the band gap energy. X-ray diffraction patterns and FT-IR investigations confirmed the formation of a single-phase M-type hexagonal structure. The grain size was measured from Scherrer's equation, and it is found in the range of 43-56 nm, with increasing the concentration of Mn ions, which shows the enhancement in the degree of crystallinity and increase in the size of grain size. UV-vis spectroscopy was used to confirm the formation of the BaZn1+x Mnx Fe12-2x O19 nanoparticles. The transmission was found to increase slowly in the 200-290 nm wavelength and then decreased to 332 nm after that increase until the maximum for all samples was about 99% in 480-800 nm wavelengths. The highest transmission will be exclusively used for window layers in solar cells. The band gap energy increased gradually with a rise in Mn ions and a decrease in Zn ion concentration. The refractive index, dielectric constant, and optical dielectric constant decreased with an increase in Mn ions and a decrease in Zn ions concentration.
{"title":"Influence of Manganese Doping on Optical Properties of Barium Ferrites Nanoparticles","authors":"","doi":"10.33263/briac134.369","DOIUrl":"https://doi.org/10.33263/briac134.369","url":null,"abstract":"The barium hexaferrite magnetic materials Mn-doped BaZn1+x Mnx Fe12-2x O19 (x = 0.4, 0.8, 1.2, 1.6, and 2) were prepared using the ceramic method. The powder samples ferrite were subjected to XRD, FT-IR, and UV-Vis to investigate the nanoparticles' structural changes. UV–Vis spectroscopy is used to obtain the samples' absorption spectrum and calculate the band gap energy. X-ray diffraction patterns and FT-IR investigations confirmed the formation of a single-phase M-type hexagonal structure. The grain size was measured from Scherrer's equation, and it is found in the range of 43-56 nm, with increasing the concentration of Mn ions, which shows the enhancement in the degree of crystallinity and increase in the size of grain size. UV-vis spectroscopy was used to confirm the formation of the BaZn1+x Mnx Fe12-2x O19 nanoparticles. The transmission was found to increase slowly in the 200-290 nm wavelength and then decreased to 332 nm after that increase until the maximum for all samples was about 99% in 480-800 nm wavelengths. The highest transmission will be exclusively used for window layers in solar cells. The band gap energy increased gradually with a rise in Mn ions and a decrease in Zn ion concentration. The refractive index, dielectric constant, and optical dielectric constant decreased with an increase in Mn ions and a decrease in Zn ions concentration.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44581693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ongoing interest of researchers in the direct-acting NS5B inhibitors in the development of viral disease hepatitis has attracted our attention in the direction of the development of a quantitative four-featured pharmacophore model containing HBA (2), HY (1), and PI (1) features. The model showed correlation coefficient, RMSD, and cost difference values as 0.895, 0.911, and 30.896, respectively. The model validation is done by using Fisher's randomization test (99%), internal and external tests set the expectation with r2 values of 0.80 and 0.65. Simultaneously, the 3D crystal structure of NS5B was utilized for generating a pharmacophore model design based on a structure with features generation 2 Hydrogen Bond Acceptors, 2 Hydrogen Bond Donors, and 2 Hydrophilic features. Further on these two models, HITS searches using NCI and Maybridge databases were done. Three hundred forty-five compounds were screened, and the three greatest potent hits were docked to the active site of NS5B. It was found that these docked conformations showed interactions with GLN446 and TYR448 amino acids, which are located at NS5B active site. In an instant, with the use of various computational methods in a sequence, we have identified novel structurally diverse NS5B inhibitors.
{"title":"Ligand and Structure-Based Pharmacophore Modelling, Computer-aided Screening and Molecular Docking to Detect Novel NS5B Polymerase Inhibitors as Anti-HCV Compounds","authors":"","doi":"10.33263/briac134.371","DOIUrl":"https://doi.org/10.33263/briac134.371","url":null,"abstract":"The ongoing interest of researchers in the direct-acting NS5B inhibitors in the development of viral disease hepatitis has attracted our attention in the direction of the development of a quantitative four-featured pharmacophore model containing HBA (2), HY (1), and PI (1) features. The model showed correlation coefficient, RMSD, and cost difference values as 0.895, 0.911, and 30.896, respectively. The model validation is done by using Fisher's randomization test (99%), internal and external tests set the expectation with r2 values of 0.80 and 0.65. Simultaneously, the 3D crystal structure of NS5B was utilized for generating a pharmacophore model design based on a structure with features generation 2 Hydrogen Bond Acceptors, 2 Hydrogen Bond Donors, and 2 Hydrophilic features. Further on these two models, HITS searches using NCI and Maybridge databases were done. Three hundred forty-five compounds were screened, and the three greatest potent hits were docked to the active site of NS5B. It was found that these docked conformations showed interactions with GLN446 and TYR448 amino acids, which are located at NS5B active site. In an instant, with the use of various computational methods in a sequence, we have identified novel structurally diverse NS5B inhibitors.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45965889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saponins have been studied for more than four decades, and their relevance is due to their numerous biological and chemical activities. Indeed, saponins are attracting attention for their industrial exploitation in connection with their pharmacological properties. Saponins also find many applications in the food and cosmetics industries due to their foaming and emulsifying properties. On the other hand, depending on the type of saponin, the species that ingest it, and the context, they are more or less toxic to the body. This article describes a number of investigations work carried out on saponins to determine the impact of saponins on health.
{"title":"The Impact of Saponins on Health-Review","authors":"","doi":"10.33263/briac134.362","DOIUrl":"https://doi.org/10.33263/briac134.362","url":null,"abstract":"Saponins have been studied for more than four decades, and their relevance is due to their numerous biological and chemical activities. Indeed, saponins are attracting attention for their industrial exploitation in connection with their pharmacological properties. Saponins also find many applications in the food and cosmetics industries due to their foaming and emulsifying properties. On the other hand, depending on the type of saponin, the species that ingest it, and the context, they are more or less toxic to the body. This article describes a number of investigations work carried out on saponins to determine the impact of saponins on health.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46609023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GABAAR is one of the most significant drug targets in the treatment of neuropsychiatric disorders such as epilepsy, insomnia, anxiety, as well as anesthesia in surgical operations. However, complete information on the regulation of GABAA receptor activity is lacking. Therefore, studies are needed on post-translational modifications of receptor subunits that exhibit different pharmacological and physiological properties. GABAAR has been immunopurified from rat brain membranes on protein A agarose beads immobilized with complex anti-GABAAR antibody. Primary structure and post-translational modifications of the α1 subunit of GABAAR have been characterized by nano-LC-FTMS peptide mapping by direct in situ–gel digestion on the one-dimensional gel. The primary structure of the α1 subunit of GABAAR has been identified with high sequence coverage (81%, 371 from 455 amino acids). The extracellular domains, N-terminal and the C-terminus, have been identified with extensive sequence coverage, 85 and 100 % (214 from 249 amino acids), respectively. Other soluble domains, including the M1-M2 linker, and the M3-M4 linker, have also been determined with 100 and 85 % (75 from 88 amino acids), respectively. Transmembrane domains, including M1, M2, and M4, were identified almost completely. Post-translational modifications of the α1 subunit peptides have been found, such as phosphorylation, methionine oxidation, and carbamidomethylation.
{"title":"Nano-LC-FTMS Analysis of the Primary Structure and Post-translational Modifications of the α1 Subunit of the GABAA Receptor","authors":"","doi":"10.33263/briac134.363","DOIUrl":"https://doi.org/10.33263/briac134.363","url":null,"abstract":"GABAAR is one of the most significant drug targets in the treatment of neuropsychiatric disorders such as epilepsy, insomnia, anxiety, as well as anesthesia in surgical operations. However, complete information on the regulation of GABAA receptor activity is lacking. Therefore, studies are needed on post-translational modifications of receptor subunits that exhibit different pharmacological and physiological properties. GABAAR has been immunopurified from rat brain membranes on protein A agarose beads immobilized with complex anti-GABAAR antibody. Primary structure and post-translational modifications of the α1 subunit of GABAAR have been characterized by nano-LC-FTMS peptide mapping by direct in situ–gel digestion on the one-dimensional gel. The primary structure of the α1 subunit of GABAAR has been identified with high sequence coverage (81%, 371 from 455 amino acids). The extracellular domains, N-terminal and the C-terminus, have been identified with extensive sequence coverage, 85 and 100 % (214 from 249 amino acids), respectively. Other soluble domains, including the M1-M2 linker, and the M3-M4 linker, have also been determined with 100 and 85 % (75 from 88 amino acids), respectively. Transmembrane domains, including M1, M2, and M4, were identified almost completely. Post-translational modifications of the α1 subunit peptides have been found, such as phosphorylation, methionine oxidation, and carbamidomethylation.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46035137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kex2 is a ~67 kD protease in yeast and fungi that specifically digest at -Lys-Arg- or -Arg-Arg-. Because of the same cleaving site, Kex2 is the potential to be applied in protein engineering, for instance, for protein purification. In the recent study, the Saccharomyces cerevisiae KEX2 gene was designed and constructed using pD902 and then integrated with the genome of Pichia pastoris BG10. Under AOX1 promoter control, Kex2 was expressed with methanol induction and found in extracellular fractions. The recombinant Kex2 was successfully confirmed with a western blot using a monoclonal anti-His-tag antibody and LC/MS/MS.
{"title":"Extracellular Expression of Recombinant Kex2 Protease in Pichia pastoris BG10","authors":"","doi":"10.33263/briac134.360","DOIUrl":"https://doi.org/10.33263/briac134.360","url":null,"abstract":"Kex2 is a ~67 kD protease in yeast and fungi that specifically digest at -Lys-Arg- or -Arg-Arg-. Because of the same cleaving site, Kex2 is the potential to be applied in protein engineering, for instance, for protein purification. In the recent study, the Saccharomyces cerevisiae KEX2 gene was designed and constructed using pD902 and then integrated with the genome of Pichia pastoris BG10. Under AOX1 promoter control, Kex2 was expressed with methanol induction and found in extracellular fractions. The recombinant Kex2 was successfully confirmed with a western blot using a monoclonal anti-His-tag antibody and LC/MS/MS.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43986426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vanadium dioxide (VO2) shows excellent thermochromic properties suitable for potential application in optical devices and smart windows coating. VO2 is interesting since actual coating on glass materials is possible along with large-scale production. This paper, VO2 nanoparticles were synthesized successfully using the sol-gel-assisted hydrothermal method. The as-prepared VO2 nanoparticles were annealed under vacuum, and the crystal phase formation, composition, morphology, and metal-insulator phase transition properties of the nanoparticles were studied. X-ray Diffraction (XRD) spectra revealed that a purely monoclinic phase of VO2 nanoparticles with an average crystallite size of 33.27 nm was synthesized. Scanning Electron Microscopy (SEM) micrographs also confirmed the formation of crystalline VO2 nanoparticles. Differential Scanning Calorimetry (DSC) curve shows that the change in temperature (∆T_c) between the exothermic and endothermic peaks is 13ºC. Moreover, Fourier Transform Infrared (FTIR) spectroscopy peaks ascertain the presence of VO2 functional groups in the synthesized nanoparticles.
{"title":"One-pot Hydrothermal Synthesis of Vanadium Dioxide Nanoparticles","authors":"","doi":"10.33263/briac134.361","DOIUrl":"https://doi.org/10.33263/briac134.361","url":null,"abstract":"Vanadium dioxide (VO2) shows excellent thermochromic properties suitable for potential application in optical devices and smart windows coating. VO2 is interesting since actual coating on glass materials is possible along with large-scale production. This paper, VO2 nanoparticles were synthesized successfully using the sol-gel-assisted hydrothermal method. The as-prepared VO2 nanoparticles were annealed under vacuum, and the crystal phase formation, composition, morphology, and metal-insulator phase transition properties of the nanoparticles were studied. X-ray Diffraction (XRD) spectra revealed that a purely monoclinic phase of VO2 nanoparticles with an average crystallite size of 33.27 nm was synthesized. Scanning Electron Microscopy (SEM) micrographs also confirmed the formation of crystalline VO2 nanoparticles. Differential Scanning Calorimetry (DSC) curve shows that the change in temperature (∆T_c) between the exothermic and endothermic peaks is 13ºC. Moreover, Fourier Transform Infrared (FTIR) spectroscopy peaks ascertain the presence of VO2 functional groups in the synthesized nanoparticles.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49472112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aflatoxins (AFs) and zearalenone (ZEN) are the most predominant mycotoxins in various food and feed. Therefore, this study aimed to estimate the antifungal activity of Saccharomyces cerevisiae strains and to study their ability to remove aflatoxin B1 (AFB1) and zearalenone (ZEN) in vitro. Data revealed that S. cerevisiae NRLL Y-12633 showed higher antifungal activity compared to S. cerevisiae NRRL Y-1089. On the other hand, S. cerevisiae suspension exhibited higher antifungal activity than S. cerevisiae supernatant. Concerning the removal of AFB1, results indicated that after 30 min. S. cerevisiae NRRL Y-1089 displayed a higher ability to remove AFB1 at a concentration of 5.0 µg/mL with a percentage of reduction reaching 87.20% than S. cerevisiae NRRL Y-12633, which removed 21.00%. As for the removal of ZEN, results showed that after 30 min. S. cerevisiae NRRL Y-12633 successfully removed ZEN at a concentration of 5.0 µg/mL by 94.80%, whereas S. cerevisiae NRRL Y-1089 removed ZEN by 91.80%. Results also indicated that the removal of AFB1 increased by increasing the incubation time, whereas the removal of ZEN decreased by increasing the incubation time. Therefore, it could be concluded that S. cerevisiae strains could be applied as an additive to decrease the concentration of mycotoxins in food and feed.
{"title":"The Efficiency of Saccharomyces Cerevisiae as an Antifungal and Antimycotoxigenic Agent","authors":"","doi":"10.33263/briac134.354","DOIUrl":"https://doi.org/10.33263/briac134.354","url":null,"abstract":"Aflatoxins (AFs) and zearalenone (ZEN) are the most predominant mycotoxins in various food and feed. Therefore, this study aimed to estimate the antifungal activity of Saccharomyces cerevisiae strains and to study their ability to remove aflatoxin B1 (AFB1) and zearalenone (ZEN) in vitro. Data revealed that S. cerevisiae NRLL Y-12633 showed higher antifungal activity compared to S. cerevisiae NRRL Y-1089. On the other hand, S. cerevisiae suspension exhibited higher antifungal activity than S. cerevisiae supernatant. Concerning the removal of AFB1, results indicated that after 30 min. S. cerevisiae NRRL Y-1089 displayed a higher ability to remove AFB1 at a concentration of 5.0 µg/mL with a percentage of reduction reaching 87.20% than S. cerevisiae NRRL Y-12633, which removed 21.00%. As for the removal of ZEN, results showed that after 30 min. S. cerevisiae NRRL Y-12633 successfully removed ZEN at a concentration of 5.0 µg/mL by 94.80%, whereas S. cerevisiae NRRL Y-1089 removed ZEN by 91.80%. Results also indicated that the removal of AFB1 increased by increasing the incubation time, whereas the removal of ZEN decreased by increasing the incubation time. Therefore, it could be concluded that S. cerevisiae strains could be applied as an additive to decrease the concentration of mycotoxins in food and feed.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45354577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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