首页 > 最新文献

BMC Veterinary Research最新文献

英文 中文
Evaluation of Praziquantel effectiveness in treating Nile tilapia clinostomid infections and its relationships to fish health and water quality : By. 评估吡喹酮治疗尼罗罗非鱼clinostomid感染的效果及其与鱼类健康和水质的关系 : 作者:By.
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-05 DOI: 10.1186/s12917-024-04279-2
Olfat A Mahdy, Marwa M Attia, Iman B Shaheed, Mohamed Abdelsalam, Mamdouh Y Elgendy, Mai A Salem

This study aimed to conduct a multidisciplinary investigation integrating detailed morphology, molecular characterization, water parameters, histopathology alteration, and the trials of treatment of Clinostomum spp. In this study, 300 Nile tilapia (Oreochromis niloticus) were collected from the farmed and wild Nile River at Al Bahr Al Aazam, Giza Governorate to assess Clinostomid infection prevalence. Fish and water samples were collected from private fish farms, and water drains at Dakahlia, and Giza, Egypt. Analysis of the water revealed inadequate water quality, particularly in the fish farms. Snails and piscivorous birds were abundant at fish collection sites. The recovered Clinostomid MCs morphological characteristics and COI gene sequence analysis identified them as Clinostomum complanatum, C. phalacrocoracis, and Euclinostomum heterostomum. Clinostomid MCs disturbed the fish's hematological and biochemical blood parameters. Bath treatment of parasitized fish with praziquantel (2 mg/L for 24 h) revealed a significant reduction in the number of vital MCs vs. infected fish (non-treated). Praziquantel (PZQ) is an effective and safe therapy for controlling Clinostomid infections affecting farmed Nile tilapia. The current findings indicate a link between poor environmental conditions and Clinostomum infections in tilapia. The study highlights the impacts of Clinostomid MCs on fish health and recommends bath treatment with PZQ as an efficient control method for these dangerous parasites to protect human and fish health.

本研究旨在开展一项多学科调查,综合详细的形态学、分子特征、水质参数、组织病理学改变和 Clinostomum spp 的治疗试验。 本研究从吉萨省 Al Bahr Al Aazam 的尼罗河养殖场和野生尼罗河中收集了 300 条尼罗罗非鱼(Oreochromis niloticus),以评估 Clinostomid 感染率。从埃及达卡利亚和吉萨的私人养鱼场和排水沟收集了鱼和水样本。水质分析表明水质不合格,尤其是在养鱼场。在鱼类采集地点,蜗牛和食鱼鸟类很多。通过对回收的桡足类 MCs 形态特征和 COI 基因序列分析,确定它们分别为桡足类 MCs、桡足类 C. phalacrocoracis 和桡足类 Euclinostomum heterostomum。Clinostomid MCs干扰了鱼类的血液和生化指标。用吡喹酮(2 毫克/升,24 小时)浸浴处理寄生鱼,发现与感染鱼(未处理)相比,有活力的 MC 数量显著减少。吡喹酮 (PZQ) 是控制尼罗罗非鱼养殖中 Clinostomid 感染的一种有效而安全的疗法。目前的研究结果表明,恶劣的环境条件与罗非鱼 Clinostomum 感染之间存在联系。该研究强调了 Clinostomid MCs 对鱼类健康的影响,并建议使用 PZQ 进行药浴治疗,作为控制这些危险寄生虫的有效方法,以保护人类和鱼类的健康。
{"title":"Evaluation of Praziquantel effectiveness in treating Nile tilapia clinostomid infections and its relationships to fish health and water quality : By.","authors":"Olfat A Mahdy, Marwa M Attia, Iman B Shaheed, Mohamed Abdelsalam, Mamdouh Y Elgendy, Mai A Salem","doi":"10.1186/s12917-024-04279-2","DOIUrl":"10.1186/s12917-024-04279-2","url":null,"abstract":"<p><p>This study aimed to conduct a multidisciplinary investigation integrating detailed morphology, molecular characterization, water parameters, histopathology alteration, and the trials of treatment of Clinostomum spp. In this study, 300 Nile tilapia (Oreochromis niloticus) were collected from the farmed and wild Nile River at Al Bahr Al Aazam, Giza Governorate to assess Clinostomid infection prevalence. Fish and water samples were collected from private fish farms, and water drains at Dakahlia, and Giza, Egypt. Analysis of the water revealed inadequate water quality, particularly in the fish farms. Snails and piscivorous birds were abundant at fish collection sites. The recovered Clinostomid MCs morphological characteristics and COI gene sequence analysis identified them as Clinostomum complanatum, C. phalacrocoracis, and Euclinostomum heterostomum. Clinostomid MCs disturbed the fish's hematological and biochemical blood parameters. Bath treatment of parasitized fish with praziquantel (2 mg/L for 24 h) revealed a significant reduction in the number of vital MCs vs. infected fish (non-treated). Praziquantel (PZQ) is an effective and safe therapy for controlling Clinostomid infections affecting farmed Nile tilapia. The current findings indicate a link between poor environmental conditions and Clinostomum infections in tilapia. The study highlights the impacts of Clinostomid MCs on fish health and recommends bath treatment with PZQ as an efficient control method for these dangerous parasites to protect human and fish health.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11453065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dogs may carry Leishmania tropica and Leishmania major in their blood circulation: a molecular and hematological study. 狗的血液循环中可能携带热带利什曼原虫和大利什曼原虫:一项分子和血液学研究。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-04 DOI: 10.1186/s12917-024-04283-6
Ali Rezaei, Hassan Sharifiyazdi, Ehsan Rakhshandehroo, Tina Yaghoobpour, Nooshin Derakhshandeh

Background: Dogs may be infected with species of Leishmania parasites that are disseminated through blood circulation and invade the internal organs. In this study, we aim to detect the parasite in the blood of dogs using the PCR technique. The present work was performed from February 2022 to May 2023 in Fars Province, southern Iran, where the disease is endemic.

Results: In total, 7(5.1%) out of 135 blood samples, six were identified as Leishmania tropica and one as Leishmania major. We found no trace of Leishmania infantum, which is always known for visceral infection. In addition, no sign of cutaneous lesions or a significant disease was seen in the animals infected with both species. Of 48 dogs with anemia, two were Leishmania positive. The mean value of hematological parameters in the infected dogs was within the normal range except for a significant reduction in the platelet measures (p < 0.05).

Conclusions: Our data revealed that both Leishmania species, tropica and major, may manifest as viscerotropic leishmaniasis. More investigations are needed to understand the conditions under which these species choose the type of infection. Moreover, our data emphasize the role of asymptomatic dogs in carrying these parasites, a crucial factor in spreading the disease.

背景:狗可能会感染利什曼原虫,这些寄生虫会通过血液循环传播并侵入内脏。本研究旨在利用 PCR 技术检测狗血液中的寄生虫。本研究于 2022 年 2 月至 2023 年 5 月在该病流行的伊朗南部法尔斯省进行:在 135 份血液样本中,共有 7 份样本(5.1%)被鉴定为热带利什曼原虫,其中 6 份样本被鉴定为主要利什曼原虫,1 份样本被鉴定为主要利什曼原虫。我们没有发现婴儿利什曼原虫的踪迹,而婴儿利什曼原虫通常被认为是内脏感染。此外,在感染这两种利什曼原虫的动物身上也没有发现皮肤病变或重大疾病的迹象。在 48 只贫血的狗中,有两只利什曼原虫呈阳性。受感染犬只的血液学参数平均值均在正常范围内,只是血小板含量显著降低(P 结论:受感染犬只的血液学参数平均值均在正常范围内,只是血小板含量显著降低(P 结论:受感染犬只的血液学参数平均值均在正常范围内):我们的数据显示,两种利什曼原虫(热带利什曼原虫和主要利什曼原虫)都可能表现为黏液性利什曼病。需要进行更多的研究,以了解这些利什曼原虫选择感染类型的条件。此外,我们的数据还强调了无症状犬在携带这些寄生虫方面的作用,这是导致疾病传播的一个关键因素。
{"title":"Dogs may carry Leishmania tropica and Leishmania major in their blood circulation: a molecular and hematological study.","authors":"Ali Rezaei, Hassan Sharifiyazdi, Ehsan Rakhshandehroo, Tina Yaghoobpour, Nooshin Derakhshandeh","doi":"10.1186/s12917-024-04283-6","DOIUrl":"10.1186/s12917-024-04283-6","url":null,"abstract":"<p><strong>Background: </strong>Dogs may be infected with species of Leishmania parasites that are disseminated through blood circulation and invade the internal organs. In this study, we aim to detect the parasite in the blood of dogs using the PCR technique. The present work was performed from February 2022 to May 2023 in Fars Province, southern Iran, where the disease is endemic.</p><p><strong>Results: </strong>In total, 7(5.1%) out of 135 blood samples, six were identified as Leishmania tropica and one as Leishmania major. We found no trace of Leishmania infantum, which is always known for visceral infection. In addition, no sign of cutaneous lesions or a significant disease was seen in the animals infected with both species. Of 48 dogs with anemia, two were Leishmania positive. The mean value of hematological parameters in the infected dogs was within the normal range except for a significant reduction in the platelet measures (p < 0.05).</p><p><strong>Conclusions: </strong>Our data revealed that both Leishmania species, tropica and major, may manifest as viscerotropic leishmaniasis. More investigations are needed to understand the conditions under which these species choose the type of infection. Moreover, our data emphasize the role of asymptomatic dogs in carrying these parasites, a crucial factor in spreading the disease.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11451050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of a teat disinfectant based on Lactococcus cremoris on the cow milk proteome. 基于 Cremoris 乳球菌的乳头消毒剂对牛奶蛋白质组的影响。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-03 DOI: 10.1186/s12917-024-04014-x
Maria Filippa Addis, Elisa Margherita Maffioli, Alessandra Gazzola, Federica Santandrea, Gabriella Tedeschi, Renata Piccinini

Background: Dairy cow milking practices require cleaning and disinfection of the teat skin before and after milking to ensure the safety and quality of milk and prevent intramammary infections. Antimicrobial proteins of natural origin can be valuable alternatives to traditional disinfectants. In a recent field trial, we demonstrated that a teat dip based on a nisin A-producing Lactococcus cremoris (L) had comparable efficacy to conventional iodophor dip (C) in preventing dairy cow mastitis. Here, we present the differential shotgun proteomics investigation of the milk collected during the trial.

Methods: Four groups of quarter milk samples with low (LSCC) and high somatic cell count (HSCC) collected at the beginning (T0) and end (TF) of the trial were analyzed for a total of 28 LSCC (14 LSCC T0 and 14 LSCC TF) and 12 HSCC (6 HSCC T0 and 6 HSCC TF) samples. Milk proteins were digested into peptides, separated by nanoHPLC, and analyzed by tandem mass spectrometry (LC-MS/MS) on an Orbitrap Fusion Tribrid mass spectrometer. The proteins were identified with MaxQuant and interaction networks of the differential proteins were investigated with STRING. The proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD045030.

Results: In healthy milk (LSCC), we detected 90 and 80 differential proteins at T0 and TF, respectively. At TF, the Lactococcus group showed higher levels of antimicrobial proteins. In mastitis milk (HSCC), we detected 88 and 106 differential proteins at T0 and TF, respectively. In the Lactococcus group, 14 proteins with antimicrobial and immune defense functions were enriched at TF vs. 4 proteins at T0. Cathelicidins were among the most relevant enriched proteins. Western immunoblotting validation confirmed the differential abundance.

Conclusions: At T0, the proteomic differences observed in healthy milk of the two groups were most likely dependent on physiological variation. On the other hand, antimicrobial and immune defense functions were higher in the milk of cows with mammary gland inflammation of the Lactococcus-treated group. Among other factors, the immunostimulatory action of nisin A might be considered as a contributor.

背景:奶牛挤奶操作要求在挤奶前后对乳头皮肤进行清洁和消毒,以确保牛奶的安全和质量,并防止乳房内感染。天然抗菌蛋白是传统消毒剂的重要替代品。在最近的一项现场试验中,我们证明了一种基于产尼生素 A 的 cremoris 乳球菌(L)的乳头浸渍剂在预防奶牛乳腺炎方面的功效与传统的碘伏浸渍剂(C)相当。在此,我们介绍了对试验期间收集的牛奶进行的差异射枪蛋白质组学调查:方法:对试验开始(T0)和结束(TF)时采集的低体细胞数(LSCC)和高体细胞数(HSCC)的四组牛奶样本进行了分析,共计 28 个 LSCC(14 个 LSCC T0 和 14 个 LSCC TF)和 12 个 HSCC(6 个 HSCC T0 和 6 个 HSCC TF)样本。牛奶蛋白质被消化成肽,经纳米高效液相色谱分离,并在 Orbitrap Fusion Tribrid 质谱仪上进行串联质谱(LC-MS/MS)分析。利用 MaxQuant 对蛋白质进行了鉴定,并利用 STRING 对差异蛋白质的相互作用网络进行了研究。蛋白质组学数据已通过PRIDE合作伙伴资源库存入蛋白质组交换联盟,数据集标识符为PXD045030:在健康牛奶(LSCC)中,我们分别在T0和TF检测到90和80个差异蛋白。在 TF 阶段,乳球菌组的抗菌蛋白含量更高。在乳腺炎牛奶(HSCC)中,我们分别在 T0 和 TF 检测到 88 和 106 种差异蛋白质。在乳球菌组中,TF富集了14种具有抗菌和免疫防御功能的蛋白质,而T0富集了4种。柔毛素是最相关的富集蛋白质之一。西方免疫印迹验证证实了丰度差异:结论:T0时,在两组健康牛奶中观察到的蛋白质组差异很可能取决于生理变化。另一方面,乳球菌治疗组乳腺炎症奶牛牛奶中的抗菌和免疫防御功能更高。除其他因素外,尼生素 A 的免疫刺激作用可能也是一个原因。
{"title":"Impact of a teat disinfectant based on Lactococcus cremoris on the cow milk proteome.","authors":"Maria Filippa Addis, Elisa Margherita Maffioli, Alessandra Gazzola, Federica Santandrea, Gabriella Tedeschi, Renata Piccinini","doi":"10.1186/s12917-024-04014-x","DOIUrl":"10.1186/s12917-024-04014-x","url":null,"abstract":"<p><strong>Background: </strong>Dairy cow milking practices require cleaning and disinfection of the teat skin before and after milking to ensure the safety and quality of milk and prevent intramammary infections. Antimicrobial proteins of natural origin can be valuable alternatives to traditional disinfectants. In a recent field trial, we demonstrated that a teat dip based on a nisin A-producing Lactococcus cremoris (L) had comparable efficacy to conventional iodophor dip (C) in preventing dairy cow mastitis. Here, we present the differential shotgun proteomics investigation of the milk collected during the trial.</p><p><strong>Methods: </strong>Four groups of quarter milk samples with low (LSCC) and high somatic cell count (HSCC) collected at the beginning (T0) and end (TF) of the trial were analyzed for a total of 28 LSCC (14 LSCC T0 and 14 LSCC TF) and 12 HSCC (6 HSCC T0 and 6 HSCC TF) samples. Milk proteins were digested into peptides, separated by nanoHPLC, and analyzed by tandem mass spectrometry (LC-MS/MS) on an Orbitrap Fusion Tribrid mass spectrometer. The proteins were identified with MaxQuant and interaction networks of the differential proteins were investigated with STRING. The proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD045030.</p><p><strong>Results: </strong>In healthy milk (LSCC), we detected 90 and 80 differential proteins at T0 and TF, respectively. At TF, the Lactococcus group showed higher levels of antimicrobial proteins. In mastitis milk (HSCC), we detected 88 and 106 differential proteins at T0 and TF, respectively. In the Lactococcus group, 14 proteins with antimicrobial and immune defense functions were enriched at TF vs. 4 proteins at T0. Cathelicidins were among the most relevant enriched proteins. Western immunoblotting validation confirmed the differential abundance.</p><p><strong>Conclusions: </strong>At T0, the proteomic differences observed in healthy milk of the two groups were most likely dependent on physiological variation. On the other hand, antimicrobial and immune defense functions were higher in the milk of cows with mammary gland inflammation of the Lactococcus-treated group. Among other factors, the immunostimulatory action of nisin A might be considered as a contributor.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11448288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of mandarin peel powder on growth, biochemical, immune, and intestinal health in Oreochromis niloticus at suboptimal temperatures. 柑橘皮粉在次优温度下对黑线鲈生长、生化、免疫和肠道健康的影响
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-02 DOI: 10.1186/s12917-024-04273-8
Rasha M Reda, Mostafa I Abd El-Rahim, Dawlat A Elkerdawy, Mohamed M M Metwally, Nermin Said

This 60-day study aimed to examine the efficacy of a diet supplemented with mandarin peel powder (MP) in enhancing the health and survival of Oreochromis niloticus under suboptimal temperature conditions (21 ℃). One hundred and eighty Nile tilapia fish (22.51 ± 0.04 g) were randomly distributed into four experimental groups; each of 3 replicates (15 fish per replicate). The first group (CONT) received a basal diet without MP. The second (MP10%), third (MP15%), and fourth (MP20%) groups were fed diets containing 10, 15, and 20% MP powder, respectively. At the end of the feeding trail, growth performance, serum growth hormone, α-amylase enzyme, lysozyme activity, nitric oxide, protease activity, globulin, serum levels of IL-1ß, antioxidant status, and intestinal histology were measured. The results showed insignificant differences between CONT, MP15%, and MP20% groups in the final body weight and specific growth rate. The growth hormones in the MP15% and MP20% groups did not show a significant difference compared to fish fed a normal basal diet (CONT). However, the amylase enzymes were significantly greater in both groups. The MP20% and MP15% groups showed a significant increase in antioxidant, lysozyme, nitric oxide, and protease activities compared to CONT. The results also showed that fish that were fed a diet with MP had significantly less of the pro-inflammatory cytokine interleukin-1 beta, and their intestinal villi got wider, especially in the MP20% group. It could be concluded that feeding tilapia on a diet with 20% MP is an effective strategy to improve their health when the temperature is below 21 °C. This is because the fish exhibit higher levels of antioxidant activity, reduced pro-inflammatory responses, and improved intestinal health without difference in the growth performance in compared to control group.

这项为期 60 天的研究旨在探讨在次优温度条件(21 ℃)下,添加柑橘皮粉(MP)的日粮对提高尼罗罗非鱼健康和存活率的功效。将 180 尾尼罗罗非鱼(22.51 ± 0.04 克)随机分为 4 个实验组,每组 3 个重复(每个重复 15 尾鱼)。第一组(CONT)接受不含 MP 的基础饲料。第二组(MP10%)、第三组(MP15%)和第四组(MP20%)分别饲喂含 10%、15% 和 20% MP 粉的饲料。饲养结束时,测定生长性能、血清生长激素、α-淀粉酶、溶菌酶活性、一氧化氮、蛋白酶活性、球蛋白、血清 IL-1ß 水平、抗氧化状态和肠道组织学。结果显示,CONT组、MP15%组和MP20%组在最终体重和特定生长率方面的差异不明显。MP15% 组和 MP20% 组的生长激素与喂食正常基础日粮(CONT)的鱼类相比没有显著差异。但是,两组的淀粉酶都明显增加。与 CONT 相比,MP20% 和 MP15% 组的抗氧化剂、溶菌酶、一氧化氮和蛋白酶活性都有显著提高。研究结果还显示,喂食 MP 的鱼类体内的促炎细胞因子白细胞介素-1 beta 明显减少,肠绒毛也变宽了,尤其是 MP20% 组。由此可以得出结论,在温度低于 21 °C时,用含 20% MP 的饲料喂养罗非鱼是改善其健康状况的有效策略。这是因为与对照组相比,罗非鱼表现出更高水平的抗氧化活性,减少了促炎症反应,改善了肠道健康,但生长性能没有差异。
{"title":"Effects of mandarin peel powder on growth, biochemical, immune, and intestinal health in Oreochromis niloticus at suboptimal temperatures.","authors":"Rasha M Reda, Mostafa I Abd El-Rahim, Dawlat A Elkerdawy, Mohamed M M Metwally, Nermin Said","doi":"10.1186/s12917-024-04273-8","DOIUrl":"10.1186/s12917-024-04273-8","url":null,"abstract":"<p><p>This 60-day study aimed to examine the efficacy of a diet supplemented with mandarin peel powder (MP) in enhancing the health and survival of Oreochromis niloticus under suboptimal temperature conditions (21 ℃). One hundred and eighty Nile tilapia fish (22.51 ± 0.04 g) were randomly distributed into four experimental groups; each of 3 replicates (15 fish per replicate). The first group (CONT) received a basal diet without MP. The second (MP10%), third (MP15%), and fourth (MP20%) groups were fed diets containing 10, 15, and 20% MP powder, respectively. At the end of the feeding trail, growth performance, serum growth hormone, α-amylase enzyme, lysozyme activity, nitric oxide, protease activity, globulin, serum levels of IL-1ß, antioxidant status, and intestinal histology were measured. The results showed insignificant differences between CONT, MP15%, and MP20% groups in the final body weight and specific growth rate. The growth hormones in the MP15% and MP20% groups did not show a significant difference compared to fish fed a normal basal diet (CONT). However, the amylase enzymes were significantly greater in both groups. The MP20% and MP15% groups showed a significant increase in antioxidant, lysozyme, nitric oxide, and protease activities compared to CONT. The results also showed that fish that were fed a diet with MP had significantly less of the pro-inflammatory cytokine interleukin-1 beta, and their intestinal villi got wider, especially in the MP20% group. It could be concluded that feeding tilapia on a diet with 20% MP is an effective strategy to improve their health when the temperature is below 21 °C. This is because the fish exhibit higher levels of antioxidant activity, reduced pro-inflammatory responses, and improved intestinal health without difference in the growth performance in compared to control group.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11446141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing diagnostic accuracy: Direct immunofluorescence assay as the gold standard for detecting Giardia duodenalis and Cryptosporidium spp. in canine and feline fecal samples. 提高诊断准确性:将直接免疫荧光测定作为检测犬猫粪便样本中十二指肠贾第虫和隐孢子虫属的黄金标准。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-02 DOI: 10.1186/s12917-024-04297-0
Juan P Barrera, Guadalupe Miró, David Carmena, Carlos Foncubierta, Juliana Sarquis, Valentina Marino, Efrén Estévez-Sánchez, Begoña Bailo, Rocío Checa, Ana Montoya

The enteric protozoan parasites Giardia duodenalis and Cryptosporidium spp. are common cause of diarrhea in pet dogs and cats, affecting primarily young animals. This comparative study evaluates the diagnostic performance of conventional and molecular methods for the detection of G. duodenalis and Cryptosporidium spp. infection in dogs and cats.The compared diagnostic assays included merthiolate-iodine-formalin (MIF) method, lateral flow immunochromatography rapid test (ICT) and real-time PCR; using direct immunofluorescence assay (DFA) as golden standard. The study included the analysis of 328 fecal samples from different dog (n = 225) and cat (n = 103) populations.According to DFA, the overall prevalence of G. duodenalis was 24.4% (80/328, 95% CI: 19.8-29.4), varying from 11.6% (12/103, 95% CI: 6.2-19.5) in cats to 30.2% (68/225, 95% CI: 24.3-36.7) in dogs. The overall prevalence of Cryptosporidium spp. was 4.0% (13/328, 95% CI: 2.1-6.7), varying from 2.9% (3/103, 95% CI: 0.6-8.3) in cats to 4.4% (10/225, 95% CI: 2.1-8.0) in dogs. MIF was only used for the detection of G. duodenalis, which was identified by this method in 22.7% of dogs and 7.8% of cats, respectively. DFA was the most sensitive technique for detecting G. duodenalis in samples from dogs and cats (p-value: < 0.001), followed by real-time PCR. Identification of Cryptosporidium infections was most effectively accomplished by the combination of DFA and PCR technique (p-value: < 0.001). In addition, epidemiological (sex, age, origin) and clinical (fecal consistency) variables were collected to assess their potential associations with an increased likelihood of infection by G. duodenalis and/or Cryptosporidium spp. Breeder dogs were more likely to harbor G. duodenalis infection (p-value: 0.004), whereas female cats were significantly more infected with Cryptosporidium (p-value: 0.003).In conclusion, DFA (alone or in combination with PCR) has been identified as the most accurate and cost-effective method for detecting G. duodenalis and Cryptosporidium spp. in fecal samples from pet dogs and cats. This highlights their importance in both veterinary and clinical settings for enabling prompt treatment and preventing potential transmission to humans.

肠道原生动物贾第虫和隐孢子虫是宠物猫狗腹泻的常见原因,主要影响幼年动物。这项比较研究评估了传统方法和分子方法在检测犬猫十二指肠贾第鞭毛虫和隐孢子虫感染方面的诊断性能。所比较的诊断方法包括梅硫二酸-碘-福尔马林(MIF)法、侧流免疫层析快速检测法(ICT)和实时 PCR;以直接免疫荧光检测法(DFA)为金标准。该研究分析了来自不同狗群(n = 225)和猫群(n = 103)的 328 份粪便样本。根据直接免疫荧光测定法,G. duodenalis 的总体流行率为 24.4%(80/328,95% CI:19.8-29.4),猫为 11.6%(12/103,95% CI:6.2-19.5),狗为 30.2%(68/225,95% CI:24.3-36.7)。隐孢子虫属的总体流行率为 4.0%(13/328,95% CI:2.1-6.7),从猫的 2.9%(3/103,95% CI:0.6-8.3)到狗的 4.4%(10/225,95% CI:2.1-8.0)不等。MIF 仅用于检测十二指肠球菌,该方法分别在 22.7% 的狗和 7.8% 的猫中发现了十二指肠球菌。DFA 是检测狗和猫样本中十二指肠杆菌最灵敏的技术(p 值:< 0.001),其次是实时 PCR。结合使用 DFA 和 PCR 技术可最有效地鉴定隐孢子虫感染(p 值:< 0.001)。此外,还收集了流行病学(性别、年龄、产地)和临床(粪便一致性)变量,以评估它们与感染十二指肠球菌和/或隐孢子虫属的可能性增加之间的潜在关联。总之,DFA(单独或与 PCR 结合使用)已被确定为检测宠物狗和宠物猫粪便样本中十二指肠球菌和隐孢子虫属的最准确、最经济的方法。这凸显了它们在兽医和临床环境中的重要性,可用于及时治疗和预防向人类的潜在传播。
{"title":"Enhancing diagnostic accuracy: Direct immunofluorescence assay as the gold standard for detecting Giardia duodenalis and Cryptosporidium spp. in canine and feline fecal samples.","authors":"Juan P Barrera, Guadalupe Miró, David Carmena, Carlos Foncubierta, Juliana Sarquis, Valentina Marino, Efrén Estévez-Sánchez, Begoña Bailo, Rocío Checa, Ana Montoya","doi":"10.1186/s12917-024-04297-0","DOIUrl":"10.1186/s12917-024-04297-0","url":null,"abstract":"<p><p>The enteric protozoan parasites Giardia duodenalis and Cryptosporidium spp. are common cause of diarrhea in pet dogs and cats, affecting primarily young animals. This comparative study evaluates the diagnostic performance of conventional and molecular methods for the detection of G. duodenalis and Cryptosporidium spp. infection in dogs and cats.The compared diagnostic assays included merthiolate-iodine-formalin (MIF) method, lateral flow immunochromatography rapid test (ICT) and real-time PCR; using direct immunofluorescence assay (DFA) as golden standard. The study included the analysis of 328 fecal samples from different dog (n = 225) and cat (n = 103) populations.According to DFA, the overall prevalence of G. duodenalis was 24.4% (80/328, 95% CI: 19.8-29.4), varying from 11.6% (12/103, 95% CI: 6.2-19.5) in cats to 30.2% (68/225, 95% CI: 24.3-36.7) in dogs. The overall prevalence of Cryptosporidium spp. was 4.0% (13/328, 95% CI: 2.1-6.7), varying from 2.9% (3/103, 95% CI: 0.6-8.3) in cats to 4.4% (10/225, 95% CI: 2.1-8.0) in dogs. MIF was only used for the detection of G. duodenalis, which was identified by this method in 22.7% of dogs and 7.8% of cats, respectively. DFA was the most sensitive technique for detecting G. duodenalis in samples from dogs and cats (p-value: < 0.001), followed by real-time PCR. Identification of Cryptosporidium infections was most effectively accomplished by the combination of DFA and PCR technique (p-value: < 0.001). In addition, epidemiological (sex, age, origin) and clinical (fecal consistency) variables were collected to assess their potential associations with an increased likelihood of infection by G. duodenalis and/or Cryptosporidium spp. Breeder dogs were more likely to harbor G. duodenalis infection (p-value: 0.004), whereas female cats were significantly more infected with Cryptosporidium (p-value: 0.003).In conclusion, DFA (alone or in combination with PCR) has been identified as the most accurate and cost-effective method for detecting G. duodenalis and Cryptosporidium spp. in fecal samples from pet dogs and cats. This highlights their importance in both veterinary and clinical settings for enabling prompt treatment and preventing potential transmission to humans.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ciliary body myxoid epithelioid sarcoma in a cat: a case report. 猫睫状体肌样上皮样肉瘤:病例报告。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-01 DOI: 10.1186/s12917-024-04286-3
Marina L Leis, Soraya Sayi, Bruce H Grahn

Background: The majority of primary, intraocular tumors in cats originate from the uvea and include feline diffuse iris melanoma, lymphoma, and iridociliary epithelial adenoma or adenocarcinoma. In this case report, we describe for the first time the clinical, histological, and immunohistochemical findings of a rare myxoid intraocular neoplasm arising from the ciliary body in a cat.

Case presentation: A 14-year-old, female, spayed domestic shorthaired cat was presented for evaluation of discolouration of the right eye. Upon examination, a clear to light whitish-tan, bubble-shaped intraocular mass adherent to the inferior ciliary body and extending into the anterior chamber was noted. Within five weeks, the tumor was significantly larger and the eye had developed secondary glaucoma so was enucleated. Light microscopic examination of the globe revealed a multinodular, hypocellular neoplasm arising from the ciliary body composed of interwoven spindle cells embedded in abundant amounts of a lightly basophilic myxoid matrix. Neoplastic cells exhibited strong immunoreactivity for cytokeratin while also showing moderate to strong immunoreactivity to vimentin. A diagnosis was therefore made of an unusual intraocular myxoid epithelioid sarcoma arising from the ciliary body.

Conclusions: Although apparently exceedingly rare, epithelioid myxosarcoma should be included as a differential diagnosis for intraocular tumors in cats and they represent a clinical, histologic, and immunohistochemical diagnostic challenge. Early surgical intervention should be considered to prevent local invasion and ascension to the brain.

背景:猫的原发性眼内肿瘤大多源自葡萄膜,包括猫弥漫性虹膜黑色素瘤、淋巴瘤和虹膜睫状上皮腺瘤或腺癌。在本病例报告中,我们首次描述了猫睫状体发生的罕见肌样眼内肿瘤的临床、组织学和免疫组化结果:一只 14 岁的雌性绝育短毛猫因右眼变色前来就诊。经检查,发现眼内有一个透明至浅白褐色的气泡状肿块,与下睫状体粘连,并延伸至前房。五周内,肿瘤明显增大,眼球出现继发性青光眼,因此进行了眼球摘除术。对眼球进行的光学显微镜检查发现,睫状体上长出了一个多结节、低细胞性肿瘤,由交织的纺锤形细胞组成,内嵌大量轻度嗜碱性肌样基质。肿瘤细胞对细胞角蛋白有很强的免疫反应性,同时对波形蛋白也有中等至很强的免疫反应性。因此,诊断结果为睫状体上长了一种不常见的眼内肌样上皮样肉瘤:上皮样肌肉瘤虽然非常罕见,但应作为猫眼内肿瘤的鉴别诊断之一,是临床、组织学和免疫组化诊断方面的难题。应考虑及早进行手术干预,以防止局部浸润和向脑部扩散。
{"title":"Ciliary body myxoid epithelioid sarcoma in a cat: a case report.","authors":"Marina L Leis, Soraya Sayi, Bruce H Grahn","doi":"10.1186/s12917-024-04286-3","DOIUrl":"10.1186/s12917-024-04286-3","url":null,"abstract":"<p><strong>Background: </strong>The majority of primary, intraocular tumors in cats originate from the uvea and include feline diffuse iris melanoma, lymphoma, and iridociliary epithelial adenoma or adenocarcinoma. In this case report, we describe for the first time the clinical, histological, and immunohistochemical findings of a rare myxoid intraocular neoplasm arising from the ciliary body in a cat.</p><p><strong>Case presentation: </strong>A 14-year-old, female, spayed domestic shorthaired cat was presented for evaluation of discolouration of the right eye. Upon examination, a clear to light whitish-tan, bubble-shaped intraocular mass adherent to the inferior ciliary body and extending into the anterior chamber was noted. Within five weeks, the tumor was significantly larger and the eye had developed secondary glaucoma so was enucleated. Light microscopic examination of the globe revealed a multinodular, hypocellular neoplasm arising from the ciliary body composed of interwoven spindle cells embedded in abundant amounts of a lightly basophilic myxoid matrix. Neoplastic cells exhibited strong immunoreactivity for cytokeratin while also showing moderate to strong immunoreactivity to vimentin. A diagnosis was therefore made of an unusual intraocular myxoid epithelioid sarcoma arising from the ciliary body.</p><p><strong>Conclusions: </strong>Although apparently exceedingly rare, epithelioid myxosarcoma should be included as a differential diagnosis for intraocular tumors in cats and they represent a clinical, histologic, and immunohistochemical diagnostic challenge. Early surgical intervention should be considered to prevent local invasion and ascension to the brain.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recommended recumbency to avoid insertional complications during small-bore wire-guided thoracostomy tube placement in cats-a cadaver study. 在猫的小孔导丝胸腔造口插管置入过程中,为避免插入并发症而建议采用的仰卧姿势--尸体研究。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-01 DOI: 10.1186/s12917-024-04301-7
Desiree Siegelmayer, Eva Schnabl-Feichter, Alexander Tichy, Gabriele Gradner, Brigitte Degasperi, Lea Liehmann

Background: Small-bore wire-guided thoracostomy tubes (SBWGTT) are commonly used in cats to manage pleural disease and generally have a low complication rate. Our study aimed to explore the correlation between recumbency of cats, placement method, and the occurrence of insertional complications to identify risk factors during SBWGTT placement. In this experimental cadaveric study, SBWGTT placement using a modified Seldinger technique was conducted in 24 feline cadavers. Cats, euthanized for reasons unrelated to the study, were randomly assigned to pleural effusion (EFF; n = 12) and pneumothorax (PNEU; n = 12) groups. Each cadaver was intubated and ventilated with a peak inspiratory pressure (PIP) of 10 mmHg, and sterile saline or air was instilled into the thorax over a 5 mm thoracoscopic trocar in the fourth intercostal space (ICS). Instillation was stopped when the lateral thoracic wall to lung distance (TWLD) reached 10 to 12 mm, measured with ultrasound in the favorable position. Sternal recumbency was the favorable position for the EFF group, and lateral recumbency for the PNEU group. Following the placement of the first SBWGTT in each group, the cadavers were positioned unfavorably (lateral recumbency for EFF group, sternal recumbency for PNEU group), and a second drain was introduced contralaterally. A bilateral 8th ICS thoracotomy was then performed to visually assess intrathoracic structures and drain integrity. A binary logistic regression mixed model was conducted to determine interaction between the induced condition and body position.

Results: A total of 48 SBWGTTs were placed, with complications observed in 33.3% (8/24) of cases. Five of these were major complications consisting of lung lacerations. Complications were more common in the unfavorable position, accounting for 75% of cases, although this result was not statistically significant. The odds of complication rates were > 70% in the unfavorable position and decreased with an increase in TWLD (< 30%).

Conclusion: Complications associated with SBWGTT placement are influenced by recumbency, although the data did not reach statistical significance. Placing cats in lateral recumbency for pneumothorax treatment and sternal recumbency for pleural effusion treatment may reduce insertional complications.

背景:小孔导丝胸腔造口管(SBWGTT)常用于猫咪胸膜疾病的治疗,并发症发生率通常较低。我们的研究旨在探讨猫的卧姿、置管方法和插入并发症发生率之间的相关性,以确定 SBWGTT 置管时的风险因素。在这项尸体实验研究中,我们在 24 只猫科动物尸体上使用改良的 Seldinger 技术进行了 SBWGTT 置管术。因与研究无关的原因被安乐死的猫被随机分配到胸腔积液组(EFF;n = 12)和气胸组(PNEU;n = 12)。每具尸体均插管通气,吸气峰压 (PIP) 为 10 mmHg,并通过第四肋间隙 (ICS) 的 5 mm 胸腔镜套管将无菌生理盐水或空气灌入胸腔。在有利体位用超声波测量侧胸壁到肺的距离(TWLD)达到 10 至 12 毫米时停止灌注。EFF组的有利体位是胸骨后卧位,PNEU组的有利体位是侧卧位。在各组放置第一个 SBWGTT 后,将尸体置于不利体位(EFF 组为侧卧位,PNEU 组为胸骨后卧位),并在对侧插入第二个引流管。然后进行双侧第 8 次 ICS 开胸手术,以直观评估胸腔内结构和引流管的完整性。采用二元逻辑回归混合模型确定诱导条件与体位之间的交互作用:共放置了 48 个 SBWGTT,33.3%(8/24)的病例出现了并发症。其中五例为肺裂伤等严重并发症。不利体位的并发症更常见,占 75%,但这一结果没有统计学意义。不利体位的并发症几率大于 70%,并随着 TWLD 的增加而降低(结论:SBWTT 相关并发症的发生率与 TWLD 的增加有关):与 SBWGTT 置放相关的并发症受侧卧位的影响,但数据未达到统计学意义。在治疗气胸时将猫置于侧卧位,在治疗胸腔积液时将猫置于胸骨后位可减少插入并发症。
{"title":"Recommended recumbency to avoid insertional complications during small-bore wire-guided thoracostomy tube placement in cats-a cadaver study.","authors":"Desiree Siegelmayer, Eva Schnabl-Feichter, Alexander Tichy, Gabriele Gradner, Brigitte Degasperi, Lea Liehmann","doi":"10.1186/s12917-024-04301-7","DOIUrl":"10.1186/s12917-024-04301-7","url":null,"abstract":"<p><strong>Background: </strong>Small-bore wire-guided thoracostomy tubes (SBWGTT) are commonly used in cats to manage pleural disease and generally have a low complication rate. Our study aimed to explore the correlation between recumbency of cats, placement method, and the occurrence of insertional complications to identify risk factors during SBWGTT placement. In this experimental cadaveric study, SBWGTT placement using a modified Seldinger technique was conducted in 24 feline cadavers. Cats, euthanized for reasons unrelated to the study, were randomly assigned to pleural effusion (EFF; n = 12) and pneumothorax (PNEU; n = 12) groups. Each cadaver was intubated and ventilated with a peak inspiratory pressure (PIP) of 10 mmHg, and sterile saline or air was instilled into the thorax over a 5 mm thoracoscopic trocar in the fourth intercostal space (ICS). Instillation was stopped when the lateral thoracic wall to lung distance (TWLD) reached 10 to 12 mm, measured with ultrasound in the favorable position. Sternal recumbency was the favorable position for the EFF group, and lateral recumbency for the PNEU group. Following the placement of the first SBWGTT in each group, the cadavers were positioned unfavorably (lateral recumbency for EFF group, sternal recumbency for PNEU group), and a second drain was introduced contralaterally. A bilateral 8th ICS thoracotomy was then performed to visually assess intrathoracic structures and drain integrity. A binary logistic regression mixed model was conducted to determine interaction between the induced condition and body position.</p><p><strong>Results: </strong>A total of 48 SBWGTTs were placed, with complications observed in 33.3% (8/24) of cases. Five of these were major complications consisting of lung lacerations. Complications were more common in the unfavorable position, accounting for 75% of cases, although this result was not statistically significant. The odds of complication rates were > 70% in the unfavorable position and decreased with an increase in TWLD (< 30%).</p><p><strong>Conclusion: </strong>Complications associated with SBWGTT placement are influenced by recumbency, although the data did not reach statistical significance. Placing cats in lateral recumbency for pneumothorax treatment and sternal recumbency for pleural effusion treatment may reduce insertional complications.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Concomitant melanoma and keratoma affecting the equine digit: clinical, pathological, and long-term follow-up findings. 影响马指骨的黑色素瘤和角化瘤并发症:临床、病理和长期随访结果。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-01 DOI: 10.1186/s12917-024-04269-4
Fernando Bulnes, David Argüelles, Antonio Buzón, Karelia García-Álamo, Irene M Rodríguez-Gómez, Eduardo Hernández, Elena Mozos
<p><strong>Background: </strong>This case report details a long-term follow-up of a hoof melanoma with dermo-epidermal activity (resembling Spreading Superficial Melanoma (SSM)) in a bay horse with a history of a right front hoof keratoma. Melanomas involving the horse's foot are seldom reported and usually diagnosed as anaplastic melanomas based on signalment and post-mortem examination. The clinical-pathological characteristics of the foot melanoma in this bay horse are consistent with SSM-like described in humans, which is considered an intermediate malignant tumour attending their biological behaviour. However, a definitive diagnosis is limited by the single case and the lack of references in horses.</p><p><strong>Case presentation: </strong>A 12-year-old bay Andalusian gelding underwent keratoma removal on the lateral aspect of the hoof wall. A partial resection of the hoof wall was performed for this purpose. Additionally, a plaque-like, hyperkeratotic pigmented lesion, 2 × 2X0,4 cm in size, was observed at the lateral aspect of the coronary band and was also resected for histopathological examination. Microscopically, a melanocytic tumour, characterised by small nests of large polygonal or epithelioid cells infiltrating the basal and suprabasal epidermis, the dermo - epidermal junction, and the superficial dermis, was observed. The neoplastic cells exhibited large euchromatic nuclei, prominent nucleoli, moderate pleomorphism and 4 mitotic figures per 2,37mm<sup>2</sup>; variable amounts of dark granules (melanin) were present in the cytoplasm, as well as in numerous peritumoral macrophages. The immunophenotype of the tumour cells was PNL2 +  +  + , S100 +  + , AE1/AE3-. A diagnosis of melanoma with dermo-epidermal junction and marked intraepidermal activity (consistent with superficial spreading melanoma) was made. A magnetic resonance imaging (MRI) performed, revealed no further invasion into surrounding structures. Treatment was based on surgical resection and multiple local chemotherapy sessions with cisplatin were applied. The biopsies obtained after treatment showed partial regression of the tumour and different stages of healing. After 26 months of follow-up, there was no signs of malignant spreading into surrounding structures including the pedal bone and distal metastasis but a dark - coloured area persists over the lateral aspect of the coronary band.</p><p><strong>Conclusions: </strong>This case presents a concomitant keratoma and melanoma with dermo - epidermal activity, resembling a spreading superficial melanoma. After a follow - up of 26 months the horse remains healthy and sound providing new information for clinicians and pathologists. Despite the poor prognosis associated with foot malignant melanocytic tumours, it is important that an early and accurate diagnosis is reached through different diagnostic modalities such as advanced imaging techniques and histopathology. Additionally, these findings demonstrate that the curr
背景:本病例报告详细介绍了对一匹曾患右前蹄角化瘤的骝马所患的具有表皮活性的蹄黑色素瘤(类似于扩散性表皮黑色素瘤 (SSM))的长期随访情况。涉及马蹄的黑色素瘤很少见报道,通常根据信号和死后检查诊断为非增生性黑色素瘤。这匹黑马足部黑色素瘤的临床病理特征与人类描述的 SSM 相似,被认为是一种符合其生物学行为的中间型恶性肿瘤。然而,由于该病例仅为一例,且缺乏马的相关参考资料,因此明确诊断受到了限制:病例介绍:一匹 12 岁的安达卢西亚骝马接受了蹄壁外侧角膜瘤切除术。为此对蹄壁进行了部分切除。此外,在冠状带外侧还发现了一个斑块状角化过度色素病变,大小为 2 × 2X 0.4 厘米,也被切除进行了组织病理学检查。显微镜下观察到黑色素细胞瘤,其特征是浸润基底和基底上表皮、真皮-表皮交界处和真皮浅层的大多角形或上皮样细胞的小巢。肿瘤细胞核大,核仁突出,呈中度多形性,每 2.37 平方毫米有 4 个有丝分裂象;细胞质和大量瘤周巨噬细胞中含有不同数量的深色颗粒(黑色素)。肿瘤细胞的免疫表型为 PNL2 + + + 、S100 + + + 、AE1/AE3-。诊断结果为黑色素瘤,具有真皮-表皮交界和明显的表皮内活动(与浅表扩散型黑色素瘤一致)。核磁共振成像(MRI)显示,瘤体没有进一步侵犯周围结构。治疗以手术切除为主,并使用顺铂进行多次局部化疗。治疗后获得的活检结果显示肿瘤部分消退,并处于不同的愈合阶段。经过 26 个月的随访,没有发现恶性肿瘤向周围结构扩散的迹象,包括蹬骨和远端转移,但冠状带外侧仍有一个深色区域:该病例同时伴有角化瘤和黑色素瘤,表皮活动,类似于扩散性浅表黑色素瘤。经过 26 个月的随访,这匹马仍然健康无恙,为临床医生和病理学家提供了新的信息。尽管足部恶性黑色素细胞瘤的预后较差,但通过先进的成像技术和组织病理学等不同诊断方式及早做出准确诊断非常重要。此外,这些研究结果还表明,目前对马足黑色素瘤的分类和预后评估还不够充分。
{"title":"Concomitant melanoma and keratoma affecting the equine digit: clinical, pathological, and long-term follow-up findings.","authors":"Fernando Bulnes, David Argüelles, Antonio Buzón, Karelia García-Álamo, Irene M Rodríguez-Gómez, Eduardo Hernández, Elena Mozos","doi":"10.1186/s12917-024-04269-4","DOIUrl":"10.1186/s12917-024-04269-4","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;This case report details a long-term follow-up of a hoof melanoma with dermo-epidermal activity (resembling Spreading Superficial Melanoma (SSM)) in a bay horse with a history of a right front hoof keratoma. Melanomas involving the horse's foot are seldom reported and usually diagnosed as anaplastic melanomas based on signalment and post-mortem examination. The clinical-pathological characteristics of the foot melanoma in this bay horse are consistent with SSM-like described in humans, which is considered an intermediate malignant tumour attending their biological behaviour. However, a definitive diagnosis is limited by the single case and the lack of references in horses.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Case presentation: &lt;/strong&gt;A 12-year-old bay Andalusian gelding underwent keratoma removal on the lateral aspect of the hoof wall. A partial resection of the hoof wall was performed for this purpose. Additionally, a plaque-like, hyperkeratotic pigmented lesion, 2 × 2X0,4 cm in size, was observed at the lateral aspect of the coronary band and was also resected for histopathological examination. Microscopically, a melanocytic tumour, characterised by small nests of large polygonal or epithelioid cells infiltrating the basal and suprabasal epidermis, the dermo - epidermal junction, and the superficial dermis, was observed. The neoplastic cells exhibited large euchromatic nuclei, prominent nucleoli, moderate pleomorphism and 4 mitotic figures per 2,37mm&lt;sup&gt;2&lt;/sup&gt;; variable amounts of dark granules (melanin) were present in the cytoplasm, as well as in numerous peritumoral macrophages. The immunophenotype of the tumour cells was PNL2 +  +  + , S100 +  + , AE1/AE3-. A diagnosis of melanoma with dermo-epidermal junction and marked intraepidermal activity (consistent with superficial spreading melanoma) was made. A magnetic resonance imaging (MRI) performed, revealed no further invasion into surrounding structures. Treatment was based on surgical resection and multiple local chemotherapy sessions with cisplatin were applied. The biopsies obtained after treatment showed partial regression of the tumour and different stages of healing. After 26 months of follow-up, there was no signs of malignant spreading into surrounding structures including the pedal bone and distal metastasis but a dark - coloured area persists over the lateral aspect of the coronary band.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;This case presents a concomitant keratoma and melanoma with dermo - epidermal activity, resembling a spreading superficial melanoma. After a follow - up of 26 months the horse remains healthy and sound providing new information for clinicians and pathologists. Despite the poor prognosis associated with foot malignant melanocytic tumours, it is important that an early and accurate diagnosis is reached through different diagnostic modalities such as advanced imaging techniques and histopathology. Additionally, these findings demonstrate that the curr","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First molecular characterisation of Hydatigera taeniaeformis (Cestoda: Taeniidae) from Rattus rattus in urban market centres from Tamale, Ghana. 从加纳塔马利城市市场中心的老鼠身上首次鉴定 Hydatigera taeniaeformis(绦虫纲:Taeniidae)的分子特征。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-09-30 DOI: 10.1186/s12917-024-04300-8
Francis Addy, Abdul-Rahman Abubakari, Abdul-Fatawu Mohammed

Background: The common cat tapeworm, Hydatigera taeniaeformis, is widely distributed across many regions of the world but its global genetic diversity, ecology, and other biological features are not fully understood. The predator-prey relationship of felids and rodents sustains the transmission in both sylvatic and synanthropic or urban settings. This study was carried out in three urban market centres in Tamale, Ghana to determine the prevalence of H. taeniaeformis infection in urban rats and characterise the local population genetics using the mitochondrial nad1 gene sequences.

Methods: From January to May 2023, rats were captured and euthanized to identity and isolate the H. taeniaeformis metacestodes. The full mitochondrial nad1 gene was amplified, sequenced and used to characterise the cestode population.

Results: In total 64/80 rats from the three market centres were found naturally infected with metacestodes of H. taeniaeformis. Averagely, infected rats harboured two cysts with one exceptional case of 25 cysts in one liver. Each cyst harboured one metacestode. The DNA sequences revealed four haplotypes in a star-like distribution network across the three market centres. All four haplotypes were found to be unique, clustered with H. taeniaeformis sensu stricto (s.s.) global isolates and had only marginal variation among themselves.

Conclusion: This is the first account of the genetic variability within H. taeniaeformis in Ghana and would contribute to ongoing and future studies on African regional biogeographic distribution of the common cat tapeworm.

背景:常见的猫带绦虫(Hydatigera taeniaeformis)广泛分布于世界许多地区,但人们对其全球遗传多样性、生态学和其他生物学特征并不完全了解。猫科动物和啮齿类动物之间的捕食与被捕食关系维持了这种绦虫在狼群和同类或城市环境中的传播。本研究在加纳塔马利的三个城市市场中心进行,以确定 H. taeniaeformis 在城市老鼠中的感染率,并利用线粒体 nad1 基因序列描述当地种群的遗传学特征:方法:从 2023 年 1 月至 5 月,捕获并安乐死大鼠,以鉴定和分离 H. taeniaeformis metacestodes。对线粒体 nad1 全基因进行扩增、测序,并用于描述绦虫种群的特征:结果:三个市场中心共有 64/80 只老鼠自然感染了 H. taeniaeformis 元绦虫。平均而言,受感染的老鼠身上有两个囊肿,其中一个特殊病例的一个肝脏内有 25 个囊肿。每个囊肿都有一个元孢子虫。DNA 序列显示,在三个市场中心的星状分布网络中有四个单倍型。所有四个单倍型都是独特的,与严格意义上的 H. taeniaeformis(s.s.)全球分离株聚集在一起,它们之间的差异很小:这是对加纳 H. taeniaeformis 遗传变异的首次描述,有助于当前和未来对非洲地区常见猫带绦虫生物地理分布的研究。
{"title":"First molecular characterisation of Hydatigera taeniaeformis (Cestoda: Taeniidae) from Rattus rattus in urban market centres from Tamale, Ghana.","authors":"Francis Addy, Abdul-Rahman Abubakari, Abdul-Fatawu Mohammed","doi":"10.1186/s12917-024-04300-8","DOIUrl":"10.1186/s12917-024-04300-8","url":null,"abstract":"<p><strong>Background: </strong>The common cat tapeworm, Hydatigera taeniaeformis, is widely distributed across many regions of the world but its global genetic diversity, ecology, and other biological features are not fully understood. The predator-prey relationship of felids and rodents sustains the transmission in both sylvatic and synanthropic or urban settings. This study was carried out in three urban market centres in Tamale, Ghana to determine the prevalence of H. taeniaeformis infection in urban rats and characterise the local population genetics using the mitochondrial nad1 gene sequences.</p><p><strong>Methods: </strong>From January to May 2023, rats were captured and euthanized to identity and isolate the H. taeniaeformis metacestodes. The full mitochondrial nad1 gene was amplified, sequenced and used to characterise the cestode population.</p><p><strong>Results: </strong>In total 64/80 rats from the three market centres were found naturally infected with metacestodes of H. taeniaeformis. Averagely, infected rats harboured two cysts with one exceptional case of 25 cysts in one liver. Each cyst harboured one metacestode. The DNA sequences revealed four haplotypes in a star-like distribution network across the three market centres. All four haplotypes were found to be unique, clustered with H. taeniaeformis sensu stricto (s.s.) global isolates and had only marginal variation among themselves.</p><p><strong>Conclusion: </strong>This is the first account of the genetic variability within H. taeniaeformis in Ghana and would contribute to ongoing and future studies on African regional biogeographic distribution of the common cat tapeworm.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Drapes in Routine Aseptic Procedures for Environmental Sustainability (project DRAPES): a protocol for a multi-centre randomised controlled trial comparing post-operative wound complication rates following routine neutering of dogs and cats using reusable or disposable surgical drapes. 环境可持续性常规无菌手术中的敷料(DRAPES 项目):多中心随机对照试验方案,比较使用可重复使用或一次性手术敷料对猫狗进行常规绝育手术后的伤口并发症发生率。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-09-28 DOI: 10.1186/s12917-024-04276-5
Nicole Dyer, Kathryn Wareham, Hannah Doit, Natalie Robinson, Jenny Stavisky, Rachel Dean, Hannah James

Background: Reusable surgical drapes have a lower lifetime environmental impact than disposable drapes in most cases. There is limited evidence regarding whether drape choice impacts patient outcomes including post-operative wound complications. The aim of this study is to compare wound complication rates following routine neutering surgeries in cats and dogs when reusable drapes are used as compared with disposable drapes.

Methods: The trial will be conducted as a pragmatic, multi-centre, parallel group randomised controlled trial in the UK. Dogs and cats undergoing routine neutering will be randomised to disposable or reusable drapes with all other aspects of care occurring as they usually would at the practice. The required sample size is 2,850, with 4750 animals to be recruited from up to ten practices to allow for a 40% loss to follow-up. Demographic data and details on peri-operative care will be collected at the time of surgery. Post-operative wound complications will be assessed and recorded as usual at each practice using clinical codes. The post-operative wound clinical codes and any antibiotic use within 30 days of surgery will be retrieved from the practice management software. The primary outcome that will be compared between the two groups is the rate of post-operative wound complications within 30 days of surgery which will be analysed by multivariable logistic regression with a binary outcome of wound complication (yes/no). Secondary outcomes are the prevalence of different types of complications and antibiotic use within 30 days of surgery which will be compared between the two groups by chi square analysis.

Discussion: Our hypothesis is that there will be no difference in post-operative wound complication rates between disposable and reusable drapes. If the likely rate of post-surgical wound complications with reusable drapes is similar to that with disposable drapes, then veterinary clinical teams can choose the more sustainable option, confident that their patients will not be impacted by this choice.

Trial registration: We have retrospectively registered the protocol on the Open Science Framework on 14 Nov 2023 (Trial registration entry: https://doi.org/10.17605/OSF.IO/72HMA ).

背景:在大多数情况下,可重复使用的手术帘布对环境的影响低于一次性帘布。关于帘布的选择是否会影响患者的预后(包括术后伤口并发症),目前证据有限。本研究的目的是比较使用可重复使用手术帘和一次性手术帘时,猫和狗常规绝育手术后的伤口并发症发生率:该试验将在英国进行,是一项务实、多中心、平行组随机对照试验。接受常规绝育手术的狗和猫将被随机分配到一次性或可重复使用的帘布中,所有其他方面的护理工作将与诊所通常的做法相同。所需样本量为 2,850 份,将从最多 10 家诊所招募 4750 只动物,以保证 40% 的随访损失。将在手术时收集人口统计学数据和围手术期护理的详细信息。术后伤口并发症将由各诊所按照惯例使用临床代码进行评估和记录。术后伤口临床代码和术后 30 天内的抗生素使用情况将从诊所管理软件中获取。两组比较的主要结果是手术后 30 天内伤口并发症的发生率,将通过多变量逻辑回归分析伤口并发症的二元结果(是/否)。次要结果是不同类型并发症的发生率和术后 30 天内抗生素的使用情况,将通过卡方分析对两组进行比较:我们的假设是,一次性和可重复使用帘布的术后伤口并发症发生率没有差异。如果可重复使用帘布的术后伤口并发症发生率与一次性帘布相似,那么兽医临床团队就可以选择更可持续的方案,并确信他们的病人不会因这一选择而受到影响:我们已于2023年11月14日在开放科学框架(Open Science Framework)上对该方案进行了回顾性注册(试验注册条目:https://doi.org/10.17605/OSF.IO/72HMA )。
{"title":"Drapes in Routine Aseptic Procedures for Environmental Sustainability (project DRAPES): a protocol for a multi-centre randomised controlled trial comparing post-operative wound complication rates following routine neutering of dogs and cats using reusable or disposable surgical drapes.","authors":"Nicole Dyer, Kathryn Wareham, Hannah Doit, Natalie Robinson, Jenny Stavisky, Rachel Dean, Hannah James","doi":"10.1186/s12917-024-04276-5","DOIUrl":"https://doi.org/10.1186/s12917-024-04276-5","url":null,"abstract":"<p><strong>Background: </strong>Reusable surgical drapes have a lower lifetime environmental impact than disposable drapes in most cases. There is limited evidence regarding whether drape choice impacts patient outcomes including post-operative wound complications. The aim of this study is to compare wound complication rates following routine neutering surgeries in cats and dogs when reusable drapes are used as compared with disposable drapes.</p><p><strong>Methods: </strong>The trial will be conducted as a pragmatic, multi-centre, parallel group randomised controlled trial in the UK. Dogs and cats undergoing routine neutering will be randomised to disposable or reusable drapes with all other aspects of care occurring as they usually would at the practice. The required sample size is 2,850, with 4750 animals to be recruited from up to ten practices to allow for a 40% loss to follow-up. Demographic data and details on peri-operative care will be collected at the time of surgery. Post-operative wound complications will be assessed and recorded as usual at each practice using clinical codes. The post-operative wound clinical codes and any antibiotic use within 30 days of surgery will be retrieved from the practice management software. The primary outcome that will be compared between the two groups is the rate of post-operative wound complications within 30 days of surgery which will be analysed by multivariable logistic regression with a binary outcome of wound complication (yes/no). Secondary outcomes are the prevalence of different types of complications and antibiotic use within 30 days of surgery which will be compared between the two groups by chi square analysis.</p><p><strong>Discussion: </strong>Our hypothesis is that there will be no difference in post-operative wound complication rates between disposable and reusable drapes. If the likely rate of post-surgical wound complications with reusable drapes is similar to that with disposable drapes, then veterinary clinical teams can choose the more sustainable option, confident that their patients will not be impacted by this choice.</p><p><strong>Trial registration: </strong>We have retrospectively registered the protocol on the Open Science Framework on 14 Nov 2023 (Trial registration entry: https://doi.org/10.17605/OSF.IO/72HMA ).</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11438356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
BMC Veterinary Research
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1