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In-Vitro Wound Healing, Antioxidants, Antibacterial and Cytotoxic Activity of Probiotic Bacteria Isolated from Spoiled Curd 腐坏凝乳中分离的益生菌体外伤口愈合、抗氧化、抗菌和细胞毒活性研究
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.076076
Lactic acid bacteria (LAB) isolated from curd has emerged as a welcome source of antimicrobials and therapeutics and is accepted as probiotics. Probiotics have been associated with improved healing of intestinal ulcers and healing of infected cutaneous wounds. Therefore this current study was undertaken to assess the antibacterial, antioxidant, minimum inhibitory concentration and cytotoxicity efficacy of Lactobacilli isolated from spoiled curd sample. The bacteria was isolated using serial dilution method and the antagonistic properties of these isolates against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were examined using agar well diffusion method. Amongst the 8 isolates, C3 exhibited the highest antibacterial activity against all the pathogens. It was then tested for antioxidant property using DPPH assay and also found wound healing property using 3T3 cell line. This review points to a new perspective and therapeutic potential of beneficial probiotic species as a safe alternative approach for treatment of patients affected by cutaneous wounds.
从凝乳中分离的乳酸菌(LAB)已成为一种受欢迎的抗菌剂和治疗药物来源,并被认为是益生菌。益生菌与改善肠道溃疡愈合和感染皮肤伤口愈合有关。因此,本研究对腐坏凝乳样品中分离的乳酸菌的抑菌、抗氧化、最低抑菌浓度和细胞毒性进行了研究。采用连续稀释法分离菌株,并用琼脂孔扩散法检测菌株对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的拮抗性能。8株分离物中,C3对所有病原菌的抑菌活性最高。然后用DPPH法检测其抗氧化性能,并用3T3细胞系检测其伤口愈合性能。这篇综述指出了有益益生菌作为治疗皮肤创伤患者的一种安全的替代方法的新观点和治疗潜力。
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引用次数: 0
Synthesis, Characterization and toxicity Analysis of Novel Silver Nanoparticles from Brassica oleracea var italica (Broccoli) 新型银纳米颗粒的合成、表征及毒性分析
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.021021
Epidemiologic studies demonstrate that vegetable consumption decreases the risk of various types of cancers. Crucifers were found to have anti-cancer and antioxidant activities due to their enriched content of glycosylates. Nanotechnology is the design, characterization, production & application of structures, devices, and systems by controlling shape and size at the nanoscale. This work describes a novel combinatorial synthesis approach, which is rapid and simple for the synthesis of metallic nanostructures of novel metals such as silver (Ag) by using a “comparison study of Conventional method (CM) and Microwave (MW) synthesis” method of silver nanoparticles. By the current study, we describe a cost-effective and environment-friendly technique for green synthesis of silver nanoparticles through the extract of Broccoli. AgNPs characterized using UV–Vis absorption spectroscopy was used to monitor the quantitative formation of silver nanoparticles. The characteristics features of the obtained silver nanoparticles were studied using a Scanning electron microscope (SEM), Transmission electron microscope (TEM), Fourier-transform infrared spectroscopy (FTIR). Here, we have reported a simple biological and low-cost approach for the preparation of stable silver nanoparticles by bioreduction of silver nitrate solution using Brassica oleracea (Broccoli) aqueous extract. The in-vitro analysis was performed in HEp 2 cell lines against which anti-cancer activity was recorded. Additionally, the antioxidant activity was also found by estimating the antioxidant enzymes. The in vivo toxicity was performed using chick embryos. The important outcome of the study will be the development of value-added products from cruciferous plants like B. oleracea for nanotechnology-based industries.
流行病学研究表明,食用蔬菜可以降低患各种癌症的风险。十字花科植物因其富含糖基酸而具有抗癌和抗氧化活性。纳米技术是通过在纳米尺度上控制形状和尺寸来设计、表征、生产和应用结构、设备和系统。本工作描述了一种新的组合合成方法,通过对银纳米粒子的“常规方法(CM)和微波(MW)合成方法的比较研究”,快速、简单地合成银(Ag)等新型金属的金属纳米结构。通过目前的研究,我们描述了一种经济、环保的利用西兰花提取物绿色合成纳米银的技术。利用紫外可见吸收光谱对AgNPs进行表征,定量监测银纳米颗粒的形成。采用扫描电子显微镜(SEM)、透射电子显微镜(TEM)、傅里叶变换红外光谱(FTIR)对制备的纳米银进行了表征。在这里,我们报道了一种简单的生物和低成本的方法,通过使用甘蓝水提取物生物还原硝酸银溶液来制备稳定的纳米银。体外分析在HEp 2细胞系中进行,并记录其抗癌活性。此外,通过对抗氧化酶的测定,还发现了其抗氧化活性。用鸡胚进行了体内毒性试验。这项研究的重要成果将是为基于纳米技术的工业开发十字花科植物的增值产品,如甘蓝。
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引用次数: 0
Purification, and Characterization of Polysaccharides of Some Plants and Utilized in Nanocarriers 几种植物多糖的纯化、表征及纳米载体应用研究
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.063063
In this study, drug-loaded nanocarrier made of plant gum polysaccharides was used for in vitro drug delivery. Purified gum was subjected to characterization and nanocarriers synthesis. Purified polysaccharides were having good antioxidant activity and antibacterial activity. The nanocarriers were synthesized using STMP, where the size was around 200 nm. It was delivering the drug good and possessing antibacterial activity.
本研究采用植物树胶多糖为载药纳米载体进行体外给药。对纯化的树胶进行了表征和纳米载体的合成。纯化后的多糖具有良好的抗氧化活性和抗菌活性。采用STMP法制备了尺寸约为200 nm的纳米载体。给药效果好,具有抗菌活性。
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引用次数: 0
Antioxidant, Anti-Diabetic and Phytochemical Screening of Dried Ficus carica Linn Fruit Powder 无花果干果粉的抗氧化、抗糖尿病及植物化学成分筛选
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.050050
Ficus carica L. or the common name is red figs that belong to the Moraceae family or the Mulberry family. Every part of the fruit or the tree has been able to treat different types of common diseases, for example, it is used as a natural laxative or a supplementary food for diabetes. Thus, this research uses four different solvents, ethyl acetate, ethanol, hexane, and water, to obtain the respective crude extracts in order to investigate the anti-diabetic properties by determining the inhibition of the activity of the diabetic enzymes, α-amylase, and α-glucosidase; and the DPPH assay determines the antioxidant activity while qualitative phytochemical screening was for phenols, alkaloids, tannins, saponins, and flavonoids; total quantitative phenolic and total flavonoid content was done. The phytochemical screening showed the presence of the compounds tested in different types of crude extracts. For the total phenolic content, ethyl acetate extract exhibits the highest content. In contrast, hexane extract shows the highest total flavonoid content. For the DPPH assay, ethyl acetate extract has the highest scavenging activity at 13.351 µg/mL with corresponding with the data of total phenolic content. For the α-glucosidase inhibitory activity, water extract has the lowest IC50 value among the four extracts but higher value than the standard. For α-amylase inhibitory activity, only ethanol extract showed the IC50 value, but it was a high value. In conclusion, there is potential for figs to be a natural source of medicine, and the extracts tested can be used for future studies.
Ficus carica L.或俗称为红色无花果,属于桑科或桑科。果实或树的每一部分都能够治疗不同类型的常见疾病,例如,它被用作天然泻药或糖尿病的补充食物。因此,本研究采用乙酸乙酯、乙醇、己烷和水四种不同的溶剂分别提取粗提物,通过测定其对糖尿病酶α-淀粉酶和α-葡萄糖苷酶活性的抑制作用来研究其抗糖尿病特性;DPPH测定抗氧化活性,定性植物化学筛选酚类、生物碱、单宁、皂苷和类黄酮;测定了总酚和总黄酮含量。植物化学筛选表明,不同类型的粗提取物中均存在所测试的化合物。总酚含量以乙酸乙酯含量最高。正己烷提取物总黄酮含量最高。在DPPH测定中,乙酸乙酯提取物的清除率最高,为13.351µg/mL,与总酚含量数据相对应。对于α-葡萄糖苷酶的抑制活性,水提物的IC50值最低,但高于标准。在α-淀粉酶抑制活性方面,只有乙醇提取物具有较高的IC50值。总之,无花果有可能成为一种天然的药物来源,并且测试的提取物可以用于未来的研究。
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引用次数: 0
Extraction and Characterization of Secondary Metabolites from Medicinal Plants 药用植物次生代谢物的提取及特性研究
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.087087
Most of the current medicines at use are derived from phyto-compounds. Plant-based products are used since ancient times to cure various ailments. Due to modern civilization, the properties of numerous medicinal plants remain undiscovered or dormant. This study deals with Cassia alata, Thespesia populnea, and Wrightia tinctoria. The extracts Cassia alata, Thespesia populnea, and Wrightia tinctoria were checked for their bioactive properties. Secondary metabolites such as alkaloids and saponins were withdrawn from dried leaves using the solvent extraction method. The secondary metabolites were characterized by UV-Vis spectroscopy (UV-Vis) and Fourier Transform Infrared Spectroscopy (FT-IR). The metabolites were evaluated for an antioxidant property using DPPH. The metabolites were further checked for larvicidal activity.
目前使用的大多数药物都是从植物化合物中提取的。自古以来,植物性产品就被用来治疗各种疾病。由于现代文明,许多药用植物的特性仍未被发现或休眠。本研究以决明子、白杨和白花为研究对象。对三种提取物分别进行了生物活性测定。采用溶剂萃取法提取干叶中的次生代谢产物生物碱和皂苷。利用紫外可见光谱(UV-Vis)和傅里叶变换红外光谱(FT-IR)对次生代谢产物进行了表征。用DPPH评价代谢物的抗氧化性能。进一步检查代谢物的杀幼虫活性。
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引用次数: 0
Efficacy of Liquid Protein Hydrolysate from Chicken Feather by Pseudomonas sp. on the Growth of Cucumis sativus 假单胞菌水解鸡毛蛋白液对黄瓜生长的影响
Pub Date : 2020-07-15 DOI: 10.33263/proceedings021.016016
Keratin, which made up the chicken feather, is difficult to be broken down by the proteolytic enzyme. Annually, millions of tons of chicken feathers are disposed of worldwide as waste without realizing the high protein content in the feather. Due to the presence of keratinase from keratinolytic bacteria, chicken feathers are disposed of together with poultry excreta. Therefore, this study is conducted to study the ability of liquid protein hydrolysate produced by bacteria in poultry excreta to utilize into biofertilizing and biocontrol. Keratinolytic bacteria are identified from poultry excreta by screening. The isolated enzyme was optimized in various conditions such as different pH, temperature, and feather concentration as well as nitrogen and carbon sources. Enzymatic activity increased gradually from 1% to 5% in carbon and nitrogen sources. Liquid protein hydrolysate was used to study the biofertilizing ability on the growth of Cucumis sativus and antibacterial effect on Escherichia coli. Pseudomonas sp. has the capability to degrade the feather on 10th day due to the high enzymatic activity. Pseudomonas sp. shows high enzymatic activity at 37⁰C, pH 8, and feather concentration at 0.5%. The chlorophyll estimation shows a p-value<0.05 after being treated with liquid protein hydrolysate. Liquid protein hydrolysate promoted the growth of Cucumis sativus as well as Pseudomonas sp. The antibacterial properties can also be seen against Escherichia coli. In a nutshell, chicken feather produces liquid protein hydrolysate, which has biofertilizing properties. The full potential of liquid hydrolysate can be understood with further analysis of peptide in protein hydrolysate.
构成鸡毛的角蛋白很难被蛋白水解酶分解。每年,全世界有数百万吨鸡毛被当作废物处理掉,却没有意识到鸡毛中的高蛋白含量。由于角化菌中含有角化酶,鸡毛与家禽排泄物一起处理。因此,本研究旨在研究家禽排泄物中细菌产生的液体蛋白水解物在生物施肥和生物防治方面的利用能力。通过筛选,从家禽排泄物中鉴定出溶角细菌。在不同的pH、温度、羽毛浓度、氮源和碳源等条件下,对分离得到的酶进行了优化。碳源和氮源的酶活性从1%到5%逐渐升高。采用液体蛋白水解液对黄瓜生长的生物增肥能力和对大肠杆菌的抑菌作用进行了研究。假单胞菌由于酶活性高,在第10天具有降解羽毛的能力。假单胞菌在37⁰C、pH 8和羽毛浓度0.5%时显示出高酶活性。水解蛋白液处理后叶绿素估计值p值<0.05。液体蛋白水解液对黄瓜和假单胞菌的生长有促进作用,对大肠杆菌也有抑菌作用。简而言之,鸡毛可以产生液体水解蛋白,具有生物肥料的特性。通过对蛋白质水解产物中肽的进一步分析,可以了解液体水解产物的全部潜力。
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引用次数: 0
A Review on Indian Medicinal Plants to Prevent Covid-19 印度预防新冠肺炎的药用植物研究进展
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.088088
Coronavirus disease (COVID-19) is an infectious disease which spreads promptly through multiple means. The viral disease in humans was first identified in Wuhan, the capital of Hubei, China. This contagious disease created a great impact and consumed the lives of many in different parts of the world. A virus gets transmitted mostly in close contact with the infected individuals, either through respiratory droplets or through handling the contaminated surface. Several attempts have been made by the researchers, and there are no vaccines developed yet. Currently, plasma from the survived individuals is used to support the infected individuals to fight against infections in many developed countries. Therefore this review focuses on the Indian medicinal plants, particularly Neem (Azadirachta indica), Pepper (Piper nigrum), Turmeric (Curcuma longa), Gale of wind (Phyllanthus amarus), etc., which has a good antiviral property. These medicinal plants are used in ancient medicines to treat diseases like Dengue virus, Hepatitis B virus, and so on. Uptake of these medicinal plants in combinations may help people to prevent them against the deadly disease and boost up their immunity.
冠状病毒病(COVID-19)是一种通过多种途径迅速传播的传染病。这种人类感染的病毒性疾病最初是在中国湖北省会武汉发现的。这种传染性疾病造成了巨大的影响,并消耗了世界各地许多人的生命。病毒主要通过与感染者密切接触传播,要么通过呼吸道飞沫传播,要么通过接触被污染的表面传播。研究人员已经做了几次尝试,但还没有开发出疫苗。目前,在许多发达国家,幸存者的血浆被用于支持感染者对抗感染。因此,本文重点介绍了印度药用植物,特别是印度楝树(Azadirachta indica)、胡椒(Piper nigrum)、姜黄(Curcuma longa)、大风(Phyllanthus amarus)等具有良好抗病毒性能的植物。这些药用植物在古代药物中用于治疗登革热病毒、乙型肝炎病毒等疾病。摄入这些药用植物的组合可以帮助人们预防致命疾病,增强免疫力。
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引用次数: 0
Method Validation & Determination of Contamination (Pesticides) Scenario of Surface & Wastewater 地表和废水污染(农药)情况的方法验证和测定
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.017017
Un-treated wastewater can contaminate surface water and harm huge amounts of life. Toxic compounds like pesticides in the wastewater can seriously disrupt aquatic ecosystems also, so there is a necessity to monitor their components. It is very important to determine these pesticides at trace levels in wastewater with an updated method. A modified liquid-liquid extraction process for micro-quantitative determination of multiclass multi-residues in surface wastewater samples of organo-chlorine (OCPs), organo-phosphorus (OPPs), synthetic pyrethroids and other pesticides by using GC-MS and GC-ECD has been established. The important method validation parameters, like the limit of detection and limit of quantification, linearity, accuracy, precision, specificity, were evaluated as per standard guidelines. The percent recovery ranged from 84.3 to 111, with an acceptable coefficient of variation (RSD) of 1.8 to 15.4%. The linearity showed a reliable range (0.995–0.999). Validated methods have been finally applied to field wastewater samples, and a significant number of water samples found to be contaminated with targeted pesticides. So, surface water pollution with special reference to pesticides has to be monitored on a regular basis has been discussed.
未经处理的废水会污染地表水,危害大量生命。废水中的农药等有毒化合物也会严重破坏水生生态系统,因此有必要对其成分进行监测。用一种新的方法测定废水中痕量农药具有重要意义。建立了一种改进的液液萃取工艺,采用气相色谱-质谱联用和气相色谱- ecd对地表废水样品中有机氯(OCPs)、有机磷(OPPs)、合成拟除虫菊酯等农药的多类多残留进行微量定量测定。对检测限、定量限、线性、准确度、精密度、专属性等重要方法验证参数按标准指南进行评价。加样回收率为84.3 ~ 111,RSD为1.8 ~ 15.4%。线性范围为0.995 ~ 0.999。经过验证的方法最终应用于现场废水样品,并发现大量水样被靶向农药污染。因此,我们讨论了地表水污染,特别是农药污染必须定期监测。
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引用次数: 0
Antibacterial Activity of Honey on Staphylococcus aureus and Streptococcus pyogenes Isolated from Wound Pus 蜂蜜对伤口脓液中金黄色葡萄球菌和化脓性链球菌的抑菌活性
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.038038
Antibacterial activity of honey obtained from an apiculture farm was tested on Staphylococcus aureus and Streptococcus pyogens isolated from wound pus was observed. Agar well diffusion method was used to determine the antibacterial activity of honey on the test organisms. Moreover, the result was that the honey sample had heavy antibacterial activities against the test organisms, which was revealed by the zone of inhibition obtained. The antibacterial activity increased with the increase in concentrations of honey and showed a clear zone for both organisms. Therefore honey can be accepted as an antibacterial agent for the treatment of wounds, burns, bedsores, and ulcers.
研究了某养蜂场蜂蜜对伤口脓液中分离的金黄色葡萄球菌和化脓性链球菌的抑菌活性。采用琼脂孔扩散法测定蜂蜜对受试菌的抑菌活性。此外,蜂蜜样品对试验生物具有较强的抑菌活性,这是由抑制区所揭示的。抗菌活性随蜂蜜浓度的增加而增加,对两种微生物都有明显的抑制作用。因此,蜂蜜可以作为治疗伤口、烧伤、褥疮和溃疡的抗菌剂。
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引用次数: 0
Quality Assessment of Gastropod Soup Powder Developed from Underutilized Chicoreus ramosus and Volegalea cochlidium 利用未充分利用的小石竹和凤尾藤制备的腹足汤粉的质量评价
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.019019
Soup powder is one of the ready to serve product, which can be easily prepared. All classes of people enjoy the delicacy of soup. Nowadays, fish soup powders and soup tablets have become an acceptable food item. The development of value-added products by incorporating meat powder with other suitable ingredients could be easily acceptable to the people. So the demand for more convenient ready – to cook and ready – to eat packaged foods are constantly increasing. Keeping this in mind, meat soup powder from C. ramosus and Volegalea cochlidium were developed and packed in different packaging materials, and the shelf life period was assessed for 240 days storage.
汤粉是一种即食食品,制作方便。所有阶层的人都喜欢喝美味的汤。如今,鱼汤粉和鱼汤片已经成为一种可以接受的食品。将肉粉与其他合适的原料混合开发增值产品是人们容易接受的。因此,对方便即食和即食包装食品的需求不断增加。在此基础上,研制了石竹肉汤粉和石竹肉汤粉,采用不同的包装材料进行包装,并对240 d的保存期进行了评价。
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引用次数: 0
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Proceedings. International Meshing Roundtable
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