Koichi Kobayashi, Makoto Sakamoto, Tatsuya Soeno, Takashi Sato
Background: Accurate measurement of bone alignment of the knee during walking provides ideal clinical information for diagnosis and treatment of knee joint disorders. To bring this ideal closer to clinical reality, we developed an image matching technique to measure the three-dimensional (3D) position of bones using anteroposterior radiography during the stance phase of treadmill walking.
Objective: To develop and validate an image matching method for evaluation of 3D knee alignment using anteroposterior radiography of artificial femoral and tibial bones.
Methods: The 3D position of each bone was recovered by minimizing the difference between the projected outline and the contour of the bone in the anteroposterior radiograph. The true value of the position was measured using a 3D coordinate measuring machine.
Results: The mean values ± standard deviation and root mean squares (RMS) of translation errors were within -1.6 ± 2.1 mm and 2.6 mm, respectively, for femur, and 2.1 ± 1.9 mm and 2.8 mm for tibia. The mean values ± standard deviation and RMS of errors in rotation were within 0.3 ± 0.7° and 0.7°, respectively, for femur, and -0.3 ± 0.9° and 0.9°, respectively, for tibia.
Conclusion: Our method is suitable for evaluating 3D knee alignment on anteroposterior radiography.
{"title":"Accuracy of an image matching technique for assessing knee alignment during the stance phase of gait using single-plane anteroposterior radiography.","authors":"Koichi Kobayashi, Makoto Sakamoto, Tatsuya Soeno, Takashi Sato","doi":"10.3233/BME-240059","DOIUrl":"10.3233/BME-240059","url":null,"abstract":"<p><strong>Background: </strong>Accurate measurement of bone alignment of the knee during walking provides ideal clinical information for diagnosis and treatment of knee joint disorders. To bring this ideal closer to clinical reality, we developed an image matching technique to measure the three-dimensional (3D) position of bones using anteroposterior radiography during the stance phase of treadmill walking.</p><p><strong>Objective: </strong>To develop and validate an image matching method for evaluation of 3D knee alignment using anteroposterior radiography of artificial femoral and tibial bones.</p><p><strong>Methods: </strong>The 3D position of each bone was recovered by minimizing the difference between the projected outline and the contour of the bone in the anteroposterior radiograph. The true value of the position was measured using a 3D coordinate measuring machine.</p><p><strong>Results: </strong>The mean values ± standard deviation and root mean squares (RMS) of translation errors were within -1.6 ± 2.1 mm and 2.6 mm, respectively, for femur, and 2.1 ± 1.9 mm and 2.8 mm for tibia. The mean values ± standard deviation and RMS of errors in rotation were within 0.3 ± 0.7° and 0.7°, respectively, for femur, and -0.3 ± 0.9° and 0.9°, respectively, for tibia.</p><p><strong>Conclusion: </strong>Our method is suitable for evaluating 3D knee alignment on anteroposterior radiography.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"522-529"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Acupuncture and moxibustion are effective in alleviating symptoms, but the large number of acupoints can make accurate needle placement and training difficult.
Objective: To address these challenges, this study aims to develop an augmented reality (AR) acupuncture navigation system designed to improve the accuracy and intuitiveness of acupoint localization.
Methods: The proposed system employs a six-point registration and positioning technique, enabling the AR navigation model to adapt to the specific characteristics of each patient.
Results: In testing, discrepancies between virtual and actual acupuncture points ranged from 0.6 mm to 3.9 mm, which is within the acceptable tolerance range for acupuncture.
Conclusion: This AR-based system shows promise in enhancing the precision of acupuncture point localization, potentially leading to improved treatment outcomes.
背景：针灸能有效缓解症状，但由于穴位众多，准确下针和训练十分困难：为了应对这些挑战，本研究旨在开发一种增强现实（AR）针灸导航系统，以提高穴位定位的准确性和直观性：方法：拟议的系统采用了六点注册和定位技术，使 AR 导航模型能够适应每位患者的具体特征：在测试中，虚拟穴位与实际穴位之间的差异从 0.6 毫米到 3.9 毫米不等，在针灸可接受的误差范围内：结论：这一基于 AR 的系统有望提高穴位定位的精确度，从而改善治疗效果。
{"title":"Acupuncture navigation method integrated with augmented reality.","authors":"Shin-Yan Chiou, Meng-Ru He","doi":"10.3233/BME-240073","DOIUrl":"10.3233/BME-240073","url":null,"abstract":"<p><strong>Background: </strong>Acupuncture and moxibustion are effective in alleviating symptoms, but the large number of acupoints can make accurate needle placement and training difficult.</p><p><strong>Objective: </strong>To address these challenges, this study aims to develop an augmented reality (AR) acupuncture navigation system designed to improve the accuracy and intuitiveness of acupoint localization.</p><p><strong>Methods: </strong>The proposed system employs a six-point registration and positioning technique, enabling the AR navigation model to adapt to the specific characteristics of each patient.</p><p><strong>Results: </strong>In testing, discrepancies between virtual and actual acupuncture points ranged from 0.6 mm to 3.9 mm, which is within the acceptable tolerance range for acupuncture.</p><p><strong>Conclusion: </strong>This AR-based system shows promise in enhancing the precision of acupuncture point localization, potentially leading to improved treatment outcomes.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"536-547"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Faisal Yaqoob, Muhammad Khizer Hayat, Muhammad Sharjeel Chaughtai, Sehrish Khan, Musa Bin Bashir
{"title":"RETRACTED: Mesenchymal stem cells derived from human adipose tissue exhibit significantly higher chondrogenic differentiation potential compared to those from rats.","authors":"Faisal Yaqoob, Muhammad Khizer Hayat, Muhammad Sharjeel Chaughtai, Sehrish Khan, Musa Bin Bashir","doi":"10.3233/BME-240062","DOIUrl":"10.3233/BME-240062","url":null,"abstract":"","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"BME240062"},"PeriodicalIF":1.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
John Z. Wu, Christopher S. Pan, Mahmood Ronaghi, Bryan M. Wimer
BACKGROUND:Wearing protective helmets is an important prevention strategy to reduce work-related traumatic brain injuries. The existing standardized testing systems are used for quality control and do not provide a quantitative measure of the helmet performance. OBJECTIVE:To analyze the failure characterizations of Type I industrial helmets and develop a generalized approach to quantify the shock absorption performance of Type I industrial helmets based on the existing standardized setups. METHODS:A representative basic Type I construction helmet model was selected for the study. Top impact tests were performed on the helmets at different drop heights using two different impactor masses (3.6 and 5.0 kg). RESULTS:When the helmets were impacted with potential impact energies smaller than the critical potential impact energy values, there was a consistent relationship between the peak impact force and the potential impact energy. When the helmets were impacted under potential impact energies greater than the critical potential impact energy values, the peak impact forces increased steeply with increasing potential impact energy. CONCLUSION:A concept of safety margin for construction helmets based on potential impact energy was introduced to quantify the helmets’ shock absorption performance. The proposed method will help helmet manufacturers improve their product quality.
背景：佩戴防护头盔是减少与工作有关的脑外伤的重要预防策略。现有的标准化测试系统用于质量控制，不能对安全帽的性能进行量化测量。目的：分析 I 型工业安全帽的失效特征，并在现有标准化设置的基础上开发一种通用方法来量化 I 型工业安全帽的减震性能。方法：研究选择了具有代表性的基本 I 型建筑安全帽模型。使用两种不同质量（3.6 千克和 5.0 千克）的冲击器，在不同下落高度对安全帽进行顶部冲击试验。结果：当安全帽受到小于临界潜在冲击能量值的冲击时，峰值冲击力与潜在冲击能量之间存在一致的关系。当头盔受到的潜在冲击能量大于临界潜在冲击能量值时，峰值冲击力随着潜在冲击能量的增加而急剧增加。结论：根据潜在冲击能量提出了建筑安全帽安全系数的概念，以量化安全帽的减震性能。所提出的方法将有助于安全帽制造商提高产品质量。
{"title":"Testing the shock protection performance of Type I construction helmets using impactors of different masses","authors":"John Z. Wu, Christopher S. Pan, Mahmood Ronaghi, Bryan M. Wimer","doi":"10.3233/bme-230173","DOIUrl":"https://doi.org/10.3233/bme-230173","url":null,"abstract":"BACKGROUND:Wearing protective helmets is an important prevention strategy to reduce work-related traumatic brain injuries. The existing standardized testing systems are used for quality control and do not provide a quantitative measure of the helmet performance. OBJECTIVE:To analyze the failure characterizations of Type I industrial helmets and develop a generalized approach to quantify the shock absorption performance of Type I industrial helmets based on the existing standardized setups. METHODS:A representative basic Type I construction helmet model was selected for the study. Top impact tests were performed on the helmets at different drop heights using two different impactor masses (3.6 and 5.0 kg). RESULTS:When the helmets were impacted with potential impact energies smaller than the critical potential impact energy values, there was a consistent relationship between the peak impact force and the potential impact energy. When the helmets were impacted under potential impact energies greater than the critical potential impact energy values, the peak impact forces increased steeply with increasing potential impact energy. CONCLUSION:A concept of safety margin for construction helmets based on potential impact energy was introduced to quantify the helmets’ shock absorption performance. The proposed method will help helmet manufacturers improve their product quality.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"11 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140799333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND:The adsorption of activated charcoal is currently a major clinical treatment for acute organophosphorus pesticide poisoning (AOPP). However, the adsorption duration and efficiency of this method is unstable. OBJECTIVE:In this study, a hydrogel embedding activated charcoal was prepared and its alleviating effects on AOPP were investigated. METHODS:A composite hydrogel using sodium alginate and polyvinyl alcohol (SA-PVA) hydrogel was prepared in this study. The structural properties of the SA-PVA hydrogel were characterized via multiple analysis including FTIR, TGA, XRD, SEM, tensile strength and expansion rate. Based on these, activated charcoal (AC) was embedded within the SA-PVA hydrogel (SA-PVA-AC) and it was used for the treatment of AOPP. RESULTS:Structural characterization indicated SA-PVA hydrogel possesses excellent mechanical properties and biocompatibility. The in vivo study demonstrated that SA-PVA-AC significantly alleviated the inflammation and oxidative damage in the liver, as evidenced by reduced levels of IL-6, TNF-α, and, IL-1β, SOD, and MDA. Furthermore, SA-PVA-AC treatment effectively re-regulated the activities of serum AST and ALT, exhibiting an improved effect on liver function. CONCLUSION:The findings suggest that activated charcoal embedded within SA-PVA hydrogel has significant potential as a therapeutic agent in treating AOPP, and offering a novel approach to managing pesticide-induced toxicity.
{"title":"Enhanced treatment of acute organophosphorus pesticide poisoning using activated charcoal-embedded sodium alginate-polyvinyl alcohol hydrogel","authors":"Li Yan, Ying Peng","doi":"10.3233/bme-240007","DOIUrl":"https://doi.org/10.3233/bme-240007","url":null,"abstract":"BACKGROUND:The adsorption of activated charcoal is currently a major clinical treatment for acute organophosphorus pesticide poisoning (AOPP). However, the adsorption duration and efficiency of this method is unstable. OBJECTIVE:In this study, a hydrogel embedding activated charcoal was prepared and its alleviating effects on AOPP were investigated. METHODS:A composite hydrogel using sodium alginate and polyvinyl alcohol (SA-PVA) hydrogel was prepared in this study. The structural properties of the SA-PVA hydrogel were characterized via multiple analysis including FTIR, TGA, XRD, SEM, tensile strength and expansion rate. Based on these, activated charcoal (AC) was embedded within the SA-PVA hydrogel (SA-PVA-AC) and it was used for the treatment of AOPP. RESULTS:Structural characterization indicated SA-PVA hydrogel possesses excellent mechanical properties and biocompatibility. The in vivo study demonstrated that SA-PVA-AC significantly alleviated the inflammation and oxidative damage in the liver, as evidenced by reduced levels of IL-6, TNF-α, and, IL-1β, SOD, and MDA. Furthermore, SA-PVA-AC treatment effectively re-regulated the activities of serum AST and ALT, exhibiting an improved effect on liver function. CONCLUSION:The findings suggest that activated charcoal embedded within SA-PVA hydrogel has significant potential as a therapeutic agent in treating AOPP, and offering a novel approach to managing pesticide-induced toxicity.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"5 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140589165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blaire V. Slavin, Nicholas A. Mirsky, Zachary M. Stauber, Vasudev Vivekanand Nayak, James E. Smay, Cristobal F. Rivera, Dindo Q. Mijares, Paulo G. Coelho, Bruce N. Cronstein, Nick Tovar, Lukasz Witek
BACKGROUND:β-tricalcium phosphate (β-TCP) has been successfully utilized as a 3D printed ceramic scaffold in the repair of non-healing bone defects; however, it requires the addition of growth factors to augment its regenerative capacity. Synthetic bone mineral (SBM) is a novel and extrudable carbonate hydroxyapatite with ionic substitutions known to facilitate bone healing. However, its efficacy as a 3D printed scaffold for hard tissue defect repair has not been explored. OBJECTIVE:To evaluate the biocompatibility and cell viability of human osteoprecursor (hOP) cells seeded on 3D printed SBM scaffolds via in vitro analysis. METHODS:SBM and β-TCP scaffolds were fabricated via 3D printing and sintered at various temperatures. Scaffolds were then subject to qualitative cytotoxicity testing and cell proliferation experiments utilizing (hOP) cells. RESULTS:SBM scaffolds sintered at lower temperatures (600 °C and 700 °C) induced greater levels of acute cellular stress. At higher sintering temperatures (1100 °C), SBM scaffolds showed inferior cellular viability relative to β-TCP scaffolds sintered to the same temperature (1100 °C). However, qualitative analysis suggested that β-TCP presented no evidence of morphological change, while SBM 1100 °C showed few instances of acute cellular stress. CONCLUSION:Results demonstrate SBM may be a promising alternative to β-TCP for potential applications in bone tissue engineering.
{"title":"3D printed β-tricalcium phosphate versus synthetic bone mineral scaffolds: A comparative in vitro study of biocompatibility","authors":"Blaire V. Slavin, Nicholas A. Mirsky, Zachary M. Stauber, Vasudev Vivekanand Nayak, James E. Smay, Cristobal F. Rivera, Dindo Q. Mijares, Paulo G. Coelho, Bruce N. Cronstein, Nick Tovar, Lukasz Witek","doi":"10.3233/bme-230214","DOIUrl":"https://doi.org/10.3233/bme-230214","url":null,"abstract":"BACKGROUND:β-tricalcium phosphate (β-TCP) has been successfully utilized as a 3D printed ceramic scaffold in the repair of non-healing bone defects; however, it requires the addition of growth factors to augment its regenerative capacity. Synthetic bone mineral (SBM) is a novel and extrudable carbonate hydroxyapatite with ionic substitutions known to facilitate bone healing. However, its efficacy as a 3D printed scaffold for hard tissue defect repair has not been explored. OBJECTIVE:To evaluate the biocompatibility and cell viability of human osteoprecursor (hOP) cells seeded on 3D printed SBM scaffolds via in vitro analysis. METHODS:SBM and β-TCP scaffolds were fabricated via 3D printing and sintered at various temperatures. Scaffolds were then subject to qualitative cytotoxicity testing and cell proliferation experiments utilizing (hOP) cells. RESULTS:SBM scaffolds sintered at lower temperatures (600 °C and 700 °C) induced greater levels of acute cellular stress. At higher sintering temperatures (1100 °C), SBM scaffolds showed inferior cellular viability relative to β-TCP scaffolds sintered to the same temperature (1100 °C). However, qualitative analysis suggested that β-TCP presented no evidence of morphological change, while SBM 1100 °C showed few instances of acute cellular stress. CONCLUSION:Results demonstrate SBM may be a promising alternative to β-TCP for potential applications in bone tissue engineering.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"22 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140589096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND:The incidence of arterial stenosis is increasing year by year. In order to better diagnose and treat arterial stenosis, numerical simulation technology has become a popular method. OBJECTIVE:A novel model is constructed to investigate the influence of microcirculation on the hemodynamicsof artery bypass graft. METHODS:In this paper, a severely narrow artery bypass graft model is considered. The geometric shape includes a narrow artery tube and a bypass graft of the same diameter with a 45° suture angle. The fluid-structure interaction model is considered by finite element numerical calculation, and the flow is simulated with microcirculation as the outlet boundary condition. The changes of blood flow velocity, pressure and wall shear stress are analyzed. RESULTS:The results show that blood almost entirely flows into the graft tube and there is no recirculation area at the anastomosis. CONCLUSION:The artery bypass graft model considering microcirculation function could simulate the physiological characteristics of blood flow more reasonably, and it provide helps for clinicians to diagnose and treat arterial stenosis.
{"title":"A hemodynamic model of artery bypass graft considering microcirculation function","authors":"Fan He, Minru Li, Lu Hua, Tingting Guo","doi":"10.3233/bme-230145","DOIUrl":"https://doi.org/10.3233/bme-230145","url":null,"abstract":"BACKGROUND:The incidence of arterial stenosis is increasing year by year. In order to better diagnose and treat arterial stenosis, numerical simulation technology has become a popular method. OBJECTIVE:A novel model is constructed to investigate the influence of microcirculation on the hemodynamicsof artery bypass graft. METHODS:In this paper, a severely narrow artery bypass graft model is considered. The geometric shape includes a narrow artery tube and a bypass graft of the same diameter with a 45° suture angle. The fluid-structure interaction model is considered by finite element numerical calculation, and the flow is simulated with microcirculation as the outlet boundary condition. The changes of blood flow velocity, pressure and wall shear stress are analyzed. RESULTS:The results show that blood almost entirely flows into the graft tube and there is no recirculation area at the anastomosis. CONCLUSION:The artery bypass graft model considering microcirculation function could simulate the physiological characteristics of blood flow more reasonably, and it provide helps for clinicians to diagnose and treat arterial stenosis.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"9 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140075869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaojiang Liu, Zongfeng Guo, Jun Li, Demo Wu, Zhongping Liu, Cheng Guan, Yixiang Guan, Xiaomin Lu
BACKGROUND: Glioblastoma is the most aggressive brain tumor with poor prognosis. Although Resveratrol (Rsv) is known to have therapeutic effects on glioma, the effects of gold-conjugated resveratrol nanoparticles (Rsv-AuNPs) on glioma cells are rarely reported. OBJECTIVE:We aimed to investigate theeffects of Rsv-AuNPs on glioma cells and its underlying mechanism. METHOD:Human glioma cell line U87 was treated with different concentrations of Rsv-AuNPs. CCK-8, transwell, and wound healing assay were performed to measure the effects of Rsv-AuNPs on cell proliferation, invasion, and migration ability, respectively. Flow cytometry assay was used to detect the effects of Rsv-AuNPs on apoptosis. Changes of protein expressions related to proliferation, invasion, migration, and apoptosis were measured by Western blot assay. In addition, the inhibitory role of Rsv-AuNPs in the PI3K/AKT/mTOR signaling pathway was verified by using PI3K inhibitor LY294002. RESULTS:Rsv-AuNPs treatment significantly suppressed proliferation, migration, and invasion of U87 cells (all P < 0.05) and increased the apoptosis rate (P < 0.05). The changes of proteins related to proliferation, migration, invasion and apoptosis were consistent (all P < 0.05). Moreover, Rsv-AuNPs treatment significantly inhibited the phosphorylation of PI3K, AKT and mTOR proteins in U87 cells (P < 0.05). CONCLUSION:The present study found that Rsv-AuNPs inhibited the proliferation, migration, and invasion of U87 cells and induced apoptosis by inhibiting the activation of PI3K/AKT/mTOR signaling pathway. In the future, Rsv-AuNPs might be applied to the clinical treatment of glioma through more in-depth animal and clinical research.
{"title":"Effect of gold-conjugated resveratrol nanoparticles on glioma cells and its underlying mechanism","authors":"Xiaojiang Liu, Zongfeng Guo, Jun Li, Demo Wu, Zhongping Liu, Cheng Guan, Yixiang Guan, Xiaomin Lu","doi":"10.3233/bme-230171","DOIUrl":"https://doi.org/10.3233/bme-230171","url":null,"abstract":"BACKGROUND: Glioblastoma is the most aggressive brain tumor with poor prognosis. Although Resveratrol (Rsv) is known to have therapeutic effects on glioma, the effects of gold-conjugated resveratrol nanoparticles (Rsv-AuNPs) on glioma cells are rarely reported. OBJECTIVE:We aimed to investigate theeffects of Rsv-AuNPs on glioma cells and its underlying mechanism. METHOD:Human glioma cell line U87 was treated with different concentrations of Rsv-AuNPs. CCK-8, transwell, and wound healing assay were performed to measure the effects of Rsv-AuNPs on cell proliferation, invasion, and migration ability, respectively. Flow cytometry assay was used to detect the effects of Rsv-AuNPs on apoptosis. Changes of protein expressions related to proliferation, invasion, migration, and apoptosis were measured by Western blot assay. In addition, the inhibitory role of Rsv-AuNPs in the PI3K/AKT/mTOR signaling pathway was verified by using PI3K inhibitor LY294002. RESULTS:Rsv-AuNPs treatment significantly suppressed proliferation, migration, and invasion of U87 cells (all P < 0.05) and increased the apoptosis rate (P < 0.05). The changes of proteins related to proliferation, migration, invasion and apoptosis were consistent (all P < 0.05). Moreover, Rsv-AuNPs treatment significantly inhibited the phosphorylation of PI3K, AKT and mTOR proteins in U87 cells (P < 0.05). CONCLUSION:The present study found that Rsv-AuNPs inhibited the proliferation, migration, and invasion of U87 cells and induced apoptosis by inhibiting the activation of PI3K/AKT/mTOR signaling pathway. In the future, Rsv-AuNPs might be applied to the clinical treatment of glioma through more in-depth animal and clinical research.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"39 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140075491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND:The clinical outcomes of bipolar radiofrequency (RF) lipolysis, a prevalent non-invasive fat reduction procedure, hinge on the delicate balance between effective lipolysis and patient safety, with skin overheating and subsequent tissue damage as primary concerns. OBJECTIVE:This study aimed to investigate a novel bipolar radiofrequency lipolysis technique, safeguarding the skin through an innovative PID temperature control algorithm. METHODS:Utilizing COMSOL Multiphysics simulation software, a two-dimensional fat and skin tissue model was established, simulating various PID temperature control schemes. The crux of the simulation involved a comparative analysis of different PID temperatures at 45 °C, 50 °C, and 55 °C and constant power strategies, assessing their implications on skin temperature. Concurrently, a custom bipolar radiofrequency lipolysis device was developed, with ex vivo experiments conducted using porcine tissue for empirical validation. RESULTS:The findings indicated that with PID settings of Kp = 7, Ki = 2, and Kd = 0, and skin temperature control at 45 °C or 50 °C, the innovative PID-based epidermal temperature control strategy successfully maintained the epidermal temperature within a safe range. This maintenance was achieved without compromising the effectiveness of RF lipolysis, significantly reducing the risk of thermal damage to the skin layers. CONCLUSION:Our research confirms the substantial practical utility of this advanced PID-based bipolar RF lipolysis technique in clinical aesthetic procedures, enhancing patient safety during adipose tissue ablation therapies.
{"title":"Simulation-guided development of advanced PID control algorithm for skin cooling in radiofrequency lipolysis","authors":"Binyu Wang, Lianru Zang, Yingxi Lu, Mengying Zhan, Tingting Sun, Yu Zhou, Chengli Song","doi":"10.3233/bme-230185","DOIUrl":"https://doi.org/10.3233/bme-230185","url":null,"abstract":"BACKGROUND:The clinical outcomes of bipolar radiofrequency (RF) lipolysis, a prevalent non-invasive fat reduction procedure, hinge on the delicate balance between effective lipolysis and patient safety, with skin overheating and subsequent tissue damage as primary concerns. OBJECTIVE:This study aimed to investigate a novel bipolar radiofrequency lipolysis technique, safeguarding the skin through an innovative PID temperature control algorithm. METHODS:Utilizing COMSOL Multiphysics simulation software, a two-dimensional fat and skin tissue model was established, simulating various PID temperature control schemes. The crux of the simulation involved a comparative analysis of different PID temperatures at 45 °C, 50 °C, and 55 °C and constant power strategies, assessing their implications on skin temperature. Concurrently, a custom bipolar radiofrequency lipolysis device was developed, with ex vivo experiments conducted using porcine tissue for empirical validation. RESULTS:The findings indicated that with PID settings of Kp = 7, Ki = 2, and Kd = 0, and skin temperature control at 45 °C or 50 °C, the innovative PID-based epidermal temperature control strategy successfully maintained the epidermal temperature within a safe range. This maintenance was achieved without compromising the effectiveness of RF lipolysis, significantly reducing the risk of thermal damage to the skin layers. CONCLUSION:Our research confirms the substantial practical utility of this advanced PID-based bipolar RF lipolysis technique in clinical aesthetic procedures, enhancing patient safety during adipose tissue ablation therapies.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"31 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140165601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND:The ultra-high molecular weight polyethylene (UHMWPE) component of artificial joints is one of the most important factors affecting the clinical outcomes of joint arthroplasty. Although the possibility of in vivo UHMWPE degradation caused by absorbed lipids has been reported, a quantitative evaluation of this phenomenon has not yet been performed. OBJECTIVE:This study aimed to establish the lipid index (LI) as a quantitative indicator of the amount of absorbed lipids and the first step to quantify their effects on UHMWPE. METHODS:The LI was defined using the infrared spectrum obtained with a Fourier-transform infrared spectrophotometer and verified using the retrieved UHMWPE components. RESULTS:The LI was consistent with the amount of extract recovered in reflux extraction with hexane. In addition, the LI could replace lipid extraction for calculating the oxidation index (OI) because the value obtained by subtracting the LI from the OI showed good agreement with the OI obtained after lipid extraction. CONCLUSION:The LI represents the amount of lipids absorbed by UHMWPE and is useful for quantitatively evaluating the effects of lipids on UHMWPE. In addition, the LI enables OI measurements that are unaffected by absorbed lipids without requiring troublesome lipid-extraction procedures.
背景：人工关节的超高分子量聚乙烯（UHMWPE）组件是影响关节置换术临床效果的最重要因素之一。虽然有报道称吸收的脂质可能导致体内超高分子量聚乙烯降解，但尚未对这一现象进行定量评估。目的：本研究旨在确定脂质指数（LI），作为吸收脂质量的定量指标和量化脂质对超高分子量聚乙烯影响的第一步。方法：使用傅立叶变换红外分光光度计获得的红外光谱来定义脂质指数，并使用回收的超高分子量聚乙烯成分进行验证。结果：LI 与正己烷回流提取中回收的提取物量一致。此外，LI 可以代替脂质萃取来计算氧化指数（OI），因为从 OI 中减去 LI 后得到的值与脂质萃取后得到的 OI 非常一致。结论：LI 表示超高分子量聚乙烯吸收的脂质量，有助于定量评估脂质对超高分子量聚乙烯的影响。此外，LI 还能测量不受吸收脂质影响的 OI，而无需麻烦的脂质提取程序。
{"title":"Lipid index to quantify the absorbed lipids in retrieved UHMWPE components of joint arthroplasty","authors":"Hideyuki Sakoda, Yusuke Tsuboko, Yoshihiro Okamoto, Eiichi Yamamoto, Takashi Imagama, Takashi Sakai, Hidetoshi Hamada, Nobuhiko Sugano","doi":"10.3233/bme-230183","DOIUrl":"https://doi.org/10.3233/bme-230183","url":null,"abstract":"BACKGROUND:The ultra-high molecular weight polyethylene (UHMWPE) component of artificial joints is one of the most important factors affecting the clinical outcomes of joint arthroplasty. Although the possibility of in vivo UHMWPE degradation caused by absorbed lipids has been reported, a quantitative evaluation of this phenomenon has not yet been performed. OBJECTIVE:This study aimed to establish the lipid index (LI) as a quantitative indicator of the amount of absorbed lipids and the first step to quantify their effects on UHMWPE. METHODS:The LI was defined using the infrared spectrum obtained with a Fourier-transform infrared spectrophotometer and verified using the retrieved UHMWPE components. RESULTS:The LI was consistent with the amount of extract recovered in reflux extraction with hexane. In addition, the LI could replace lipid extraction for calculating the oxidation index (OI) because the value obtained by subtracting the LI from the OI showed good agreement with the OI obtained after lipid extraction. CONCLUSION:The LI represents the amount of lipids absorbed by UHMWPE and is useful for quantitatively evaluating the effects of lipids on UHMWPE. In addition, the LI enables OI measurements that are unaffected by absorbed lipids without requiring troublesome lipid-extraction procedures.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"18 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139551715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号