The World Health Organization (WHO) defines congenital malformation as any gross abnormality present at birth even if it is not immediately recognized. Here, we aimed to identify the factors predisposing to congenital malformations in pregnant women in Eastern Algeria and the effect of the combination of several factors. 786 pregnant women were interviewed. Throughout our study, we sought to find answers, discuss the association between malformation exposure, identify the risk factors, and predict the occurrence of malformations using statistical analysis techniques. We developed a predictive model and we found that malformations were significantly higher for pregnant women aged over 34 (10.93; 95%CI: 2.10-201.71, p=0.023), with a BMI over 25 (2.38; 0.91-7.04, p=0.091), hypertension (2.18; 1.04-4.57, p=0.038), anemia (2.08; 0.98-4.35, p=0.053) and infections (2.32; 1.18-4.58; p=0.015). Protecting factors against malformations includes diet moderate in meat (1.97; 0.73-4.98, p=0.163) and rich in eggs, soft drinks, and fruit-vegetables (2.69; 0.76-8.63, p=0.106), (1.84; 0.74-4.43, p=0.177), (3.08; 0.56-13.28, p=0.154); respectively. Knowing risk factors helps to establish predictive models and strategies to prevent malformations and highlights the link between malformations and multiple risk factors for the sake of protecting babies’ lives.
{"title":"Prevention of congenital malformations for pregnant women: a predictive model based on a study in eastern Algeria’s population","authors":"Adel Kalla, L. Loucif, M. Yahia","doi":"10.4081/JBR.2021.9394","DOIUrl":"https://doi.org/10.4081/JBR.2021.9394","url":null,"abstract":"The World Health Organization (WHO) defines congenital malformation as any gross abnormality present at birth even if it is not immediately recognized. Here, we aimed to identify the factors predisposing to congenital malformations in pregnant women in Eastern Algeria and the effect of the combination of several factors. 786 pregnant women were interviewed. Throughout our study, we sought to find answers, discuss the association between malformation exposure, identify the risk factors, and predict the occurrence of malformations using statistical analysis techniques. We developed a predictive model and we found that malformations were significantly higher for pregnant women aged over 34 (10.93; 95%CI: 2.10-201.71, p=0.023), with a BMI over 25 (2.38; 0.91-7.04, p=0.091), hypertension (2.18; 1.04-4.57, p=0.038), anemia (2.08; 0.98-4.35, p=0.053) and infections (2.32; 1.18-4.58; p=0.015). Protecting factors against malformations includes diet moderate in meat (1.97; 0.73-4.98, p=0.163) and rich in eggs, soft drinks, and fruit-vegetables (2.69; 0.76-8.63, p=0.106), (1.84; 0.74-4.43, p=0.177), (3.08; 0.56-13.28, p=0.154); respectively. Knowing risk factors helps to establish predictive models and strategies to prevent malformations and highlights the link between malformations and multiple risk factors for the sake of protecting babies’ lives.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84740004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Chroho, A. Drioiche, Soukaina Saidi, T. Zair, L. Bouissane
The search for natural antioxidants to replace synthetic antioxidants is one of humanity’s health priorities. Medicinal and Aromatic Plants (MAP) contain phenolic compounds that act as natural antioxidants. The aim of this work is to study total phenols and flavonoids contents and evaluate the antioxidant power of various extracts of Calendula officinalis. Extracts were prepared in two steps: hot solid-liquid extraction, by Soxhlet and from the plant powder using a solvent (Methanol/water 70/30, Acetone/water 70/30) followed by a liquid-liquid extraction by splitting the obtained extract by two increasingly polarized solvents (Ethyl acetate and Butanol). Total phenols and flavonoids were evaluated using gallic acid and quercetin as standards, respectively. The antioxidant activity of extracts was determined by DPPH (2,2-diphényl 1-picrylhydrazyle) free radical reduction method. The two crude fractions of Methanol and Acetone presented the highest levels of total phenols (7,58±0,38 mg GAE/g dm and 6,80±0,34 mg GAE/g dm respectively), and flavonoids (7,37±0,37 mg QE/g dm and 7,93±0,40 mg QE/g dm respectively). Ethyl acetate fractions showed a high antioxidant activity. This study demonstrated that extracts from Calendula officinalis flowers present a promising source of natural antioxidant.
{"title":"Total phenolic and flavonoids contents and in vitro evaluation of antioxidant activity of several Calendula officinalis (Marigold) extracts","authors":"M. Chroho, A. Drioiche, Soukaina Saidi, T. Zair, L. Bouissane","doi":"10.4081/JBR.2021.9680","DOIUrl":"https://doi.org/10.4081/JBR.2021.9680","url":null,"abstract":"The search for natural antioxidants to replace synthetic antioxidants is one of humanity’s health priorities. Medicinal and Aromatic Plants (MAP) contain phenolic compounds that act as natural antioxidants. The aim of this work is to study total phenols and flavonoids contents and evaluate the antioxidant power of various extracts of Calendula officinalis. Extracts were prepared in two steps: hot solid-liquid extraction, by Soxhlet and from the plant powder using a solvent (Methanol/water 70/30, Acetone/water 70/30) followed by a liquid-liquid extraction by splitting the obtained extract by two increasingly polarized solvents (Ethyl acetate and Butanol). Total phenols and flavonoids were evaluated using gallic acid and quercetin as standards, respectively. The antioxidant activity of extracts was determined by DPPH (2,2-diphényl 1-picrylhydrazyle) free radical reduction method. The two crude fractions of Methanol and Acetone presented the highest levels of total phenols (7,58±0,38 mg GAE/g dm and 6,80±0,34 mg GAE/g dm respectively), and flavonoids (7,37±0,37 mg QE/g dm and 7,93±0,40 mg QE/g dm respectively). Ethyl acetate fractions showed a high antioxidant activity. This study demonstrated that extracts from Calendula officinalis flowers present a promising source of natural antioxidant.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89674355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sayfulla G. Boboyev, Gulnoza Toshpulatova, I. Amanturdiev, M. Mirakhmedov
In this paper presents data of researches about fiber length in the 4-5 species. The largest number of plants with the indicated analyzed trait in hybrid combinations, obtained with the participation of the variety Omad, appeared with a number of variations in fiber length at 35,0-36,0 mm and above. Investigation of 4-species hybrids of F2 and back cross hybridization allowed some improvement in the average value of trait when compared with F1. Despite the emergence of transgressive plants with fiber length 36,1-37,0 mm and above, the average trait of composite 5 species hybrids F2 remained almost at the level of F1, i.e. 35.5-36.0 mm. The greatest number of positive recombinants occurred in 5 species back crossing developed with the participation of Termez-31.
{"title":"Variability and inheritance of fiber length and wilt resistance in a complex 4-5 specific and backcross hybridization of cotton","authors":"Sayfulla G. Boboyev, Gulnoza Toshpulatova, I. Amanturdiev, M. Mirakhmedov","doi":"10.4081/JBR.2021.9243","DOIUrl":"https://doi.org/10.4081/JBR.2021.9243","url":null,"abstract":"In this paper presents data of researches about fiber length in the 4-5 species. The largest number of plants with the indicated analyzed trait in hybrid combinations, obtained with the participation of the variety Omad, appeared with a number of variations in fiber length at 35,0-36,0 mm and above. Investigation of 4-species hybrids of F2 and back cross hybridization allowed some improvement in the average value of trait when compared with F1. Despite the emergence of transgressive plants with fiber length 36,1-37,0 mm and above, the average trait of composite 5 species hybrids F2 remained almost at the level of F1, i.e. 35.5-36.0 mm. The greatest number of positive recombinants occurred in 5 species back crossing developed with the participation of Termez-31.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85238959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Nadi, M. Bayat, Hadi Karami, M. Parastesh, P. Bayat
Adiponectin and its receptors are expressed in the male reproductive system, which play a role in regulating male sex hormones and fertility. Diabetes was induced by Streptozotocin-Nicotinamide (STZ-NA i.p) in rats and after performing the trainings, adiponectin gene expression and its receptors in the testis were evaluated using real time PCR, and blood serum was then used in order to assess FSH, LH and testosterone. The STZ-NA significantly increased the fasting blood glucose, gene expression of adiponectin AdipoR1 in the testicles of diabetic rats. A significant reduction in serum testosterone and LH levels were observed in the diabetic group. Resistance and endurance training decreased blood glucose, adiponectin and AdipoR1 gene, and also increased the serum testosterone and LH levels in diabetic rats. Overall, our data suggest the role played by training in improve expression of adiponectin and AdipoR1 gene by increasing the serum testosterone and LH levels in type 2 diabetic rats.
脂联素及其受体在男性生殖系统中表达，在调节男性性激素和生育能力中起作用。采用链脲唑素-烟酰胺(STZ-NA i.p)诱导大鼠糖尿病，训练结束后采用real - time PCR检测大鼠睾丸脂联素基因及其受体的表达，并测定血清FSH、LH和睾酮水平。STZ-NA显著提高糖尿病大鼠空腹血糖、睾丸脂联素AdipoR1基因表达。糖尿病组血清睾酮和黄体生成素水平显著降低。抵抗和耐力训练降低糖尿病大鼠血糖、脂联素和AdipoR1基因，提高血清睾酮和LH水平。总的来说，我们的数据表明，训练通过增加2型糖尿病大鼠的血清睾酮和LH水平来改善脂联素和AdipoR1基因的表达。
{"title":"Effect of Exercise Training on Gene Expression of Adiponectin and its Receptors in Testicles and Sex Hormones in Diabetic Rats","authors":"Z. Nadi, M. Bayat, Hadi Karami, M. Parastesh, P. Bayat","doi":"10.4081/JBR.2021.9287","DOIUrl":"https://doi.org/10.4081/JBR.2021.9287","url":null,"abstract":"Adiponectin and its receptors are expressed in the male reproductive system, which play a role in regulating male sex hormones and fertility. Diabetes was induced by Streptozotocin-Nicotinamide (STZ-NA i.p) in rats and after performing the trainings, adiponectin gene expression and its receptors in the testis were evaluated using real time PCR, and blood serum was then used in order to assess FSH, LH and testosterone. The STZ-NA significantly increased the fasting blood glucose, gene expression of adiponectin AdipoR1 in the testicles of diabetic rats. A significant reduction in serum testosterone and LH levels were observed in the diabetic group. Resistance and endurance training decreased blood glucose, adiponectin and AdipoR1 gene, and also increased the serum testosterone and LH levels in diabetic rats. Overall, our data suggest the role played by training in improve expression of adiponectin and AdipoR1 gene by increasing the serum testosterone and LH levels in type 2 diabetic rats. \u0000 \u0000 ","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77822701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Raksha, T. Halenova, T. Vovk, O. Kharchenko, O. Savchuk, I. Samborska, N. Zaichko, L. Ostapchenko, O. Maievskyi
The accumulated data indicate that a high level of homocysteine may be a central pathogenetic factor of chronic obstructive pulmonary disease. In this study, we investigated the effect of hyperhomocysteinemia on protein homeostasis in the rat lungs. The level of proteins, peptides, total proteolytic activity, as well as protein-peptide composition, were evaluated. Hyperhomocysteinemia was induced by daily intragastric administration of DL-homocysteine thiolactone (100 mg·kg-1 of body weight) to albino non-linear male rats for 28 days. Twelve hours after the last administration, the rats were sacrificed and the lungs were harvested. Our findings showed that hyperhomocysteinemia caused the disturbances in the protein homeostasis in the lungs that are manifested by a decrease in the level of proteins in the young and old animals and an increase in the level of peptides in the rats of all studied groups. We found a change in the protein composition in the lung of HM rats - a decrease in the level of proteins with a molecular weight of 50 kDa to 100 kDa simultaneously with an increase in the level of proteins with a molecular weight of less than 50 kDa. Despite the fact that the peptide profile was the same in both control animals and HM animals, the level of individual peptide fractions increased significantly in the rats with HM. Obtained data could contribute to explain, at least in part, the mechanisms involved in the pathogenesis of lung damage in hyperhomocysteinemia.
{"title":"Protein-peptide composition in the lungs of rats with hyperhomocysteinemia","authors":"N. Raksha, T. Halenova, T. Vovk, O. Kharchenko, O. Savchuk, I. Samborska, N. Zaichko, L. Ostapchenko, O. Maievskyi","doi":"10.4081/jbr.2021.9858","DOIUrl":"https://doi.org/10.4081/jbr.2021.9858","url":null,"abstract":"The accumulated data indicate that a high level of homocysteine may be a central pathogenetic factor of chronic obstructive pulmonary disease. In this study, we investigated the effect of hyperhomocysteinemia on protein homeostasis in the rat lungs. The level of proteins, peptides, total proteolytic activity, as well as protein-peptide composition, were evaluated. Hyperhomocysteinemia was induced by daily intragastric administration of DL-homocysteine thiolactone (100 mg·kg-1 of body weight) to albino non-linear male rats for 28 days. Twelve hours after the last administration, the rats were sacrificed and the lungs were harvested. Our findings showed that hyperhomocysteinemia caused the disturbances in the protein homeostasis in the lungs that are manifested by a decrease in the level of proteins in the young and old animals and an increase in the level of peptides in the rats of all studied groups. We found a change in the protein composition in the lung of HM rats - a decrease in the level of proteins with a molecular weight of 50 kDa to 100 kDa simultaneously with an increase in the level of proteins with a molecular weight of less than 50 kDa. Despite the fact that the peptide profile was the same in both control animals and HM animals, the level of individual peptide fractions increased significantly in the rats with HM. Obtained data could contribute to explain, at least in part, the mechanisms involved in the pathogenesis of lung damage in hyperhomocysteinemia.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77520594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sea cucumbers are marine invertebrates commonly found in benthic areas and deep seas. On a global scale, they have a high commercial value with an increased level of production and trade. This study aims to analyze the bioactive compound in Sea cucumber (Muelleria lecanora) using the Ultrasound-Assisted Extraction Method (UAE) and Gas Chromatography-Mass Spectrometry (GC-MS). Furthermore, it identifies the antibacterial activity in microorganisms Salmonella, Escherichia coli, and Staphylococcus aureus. The bioactive compounds were extracted using methanol, acetone, and n-hexane solvent and were separated by ultrasound-assisted extraction. In the initial stage, phytochemicals were screened using Gas Chromatography-Mass Spectrometry (GC-MS). Disc diffusion method was then used to determine the antibacterial activity against Salmonella, Staphylococcus aureus, and Escherichia coli. The results showed that methanol extract is more suitable for extracting bioactive compounds of Muelleria lecanora than acetone and n-hexane. Meanwhile, acetone solvents are more suitable for the production of flavonoid and steroid compounds than Mulleria lecanora samples. Heneicosane compounds that function as a new antiproliferative for inhibition of tumor and cancerous cells are produced from n-hexane. The antibacterial activity of acetone, methanol and n-hexane extract determined by diffusion assay was effective against Staphylococcus aureus and Salmonella but ineffective against Escherichia coli. GC-MS results showed that the major constituents obtained were steroid and flavanoid. From this study, Sea cucumber extract can be considered a healthy nutrient in food and pharmaceutical products.
{"title":"GC-MS analysis and antibacterial activity of the Sea cucumber (Muelleria lecanora) extract","authors":"M. Yusuf, Nur Fitriani Usdyana Attahmid","doi":"10.4081/jbr.2021.9765","DOIUrl":"https://doi.org/10.4081/jbr.2021.9765","url":null,"abstract":"Sea cucumbers are marine invertebrates commonly found in benthic areas and deep seas. On a global scale, they have a high commercial value with an increased level of production and trade. This study aims to analyze the bioactive compound in Sea cucumber (Muelleria lecanora) using the Ultrasound-Assisted Extraction Method (UAE) and Gas Chromatography-Mass Spectrometry (GC-MS). Furthermore, it identifies the antibacterial activity in microorganisms Salmonella, Escherichia coli, and Staphylococcus aureus. The bioactive compounds were extracted using methanol, acetone, and n-hexane solvent and were separated by ultrasound-assisted extraction. In the initial stage, phytochemicals were screened using Gas Chromatography-Mass Spectrometry (GC-MS). Disc diffusion method was then used to determine the antibacterial activity against Salmonella, Staphylococcus aureus, and Escherichia coli. The results showed that methanol extract is more suitable for extracting bioactive compounds of Muelleria lecanora than acetone and n-hexane. Meanwhile, acetone solvents are more suitable for the production of flavonoid and steroid compounds than Mulleria lecanora samples. Heneicosane compounds that function as a new antiproliferative for inhibition of tumor and cancerous cells are produced from n-hexane. The antibacterial activity of acetone, methanol and n-hexane extract determined by diffusion assay was effective against Staphylococcus aureus and Salmonella but ineffective against Escherichia coli. GC-MS results showed that the major constituents obtained were steroid and flavanoid. From this study, Sea cucumber extract can be considered a healthy nutrient in food and pharmaceutical products.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89075402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. S. Elshafie, M. Nuzzaci, G. Logozzo, Tania Gioia, I. Camele
Recent scientific research have manipulated the use of hydrogel in seed coating technology based on synthetic and chemical additives. The current study has been carried out to evaluate the biological activity of new seed coating formulations containing hydrogel based on natural substances or beneficial microorganisms on seed germination and controlling some common diseases of Phaseolus vulgaris L. New formulations have been prepared as single mixtures of hydrogel with the following bioactive substances: i) oregano Essential Oil (org EO); ii) Ornithine Lipid (OL); and two microorganisms i) Burkholderia gladioli and ii) Trichoderma harzianum T22. Results revealed that, the hydrogel formulation based org EO showed the highest significant activity against the majority of tested phytopathogens in a dose dependent manner. Regarding the antagonistic microbial activity, results showed that hydrogel formulations based T. harzianum T22 and B. gladioli were able to significantly reduce the growth of the majority of tested phytopathogens. In addition, the highest significant percentage of seed germination has been achieved using the formulations of B. gladioli and org EO. Regarding the disease incidence suppression assay, results explicated that org EO and OL were able to significantly inhibit the fungal disease incidence on P. vulgaris seeds steadily depending on the tested concentrations. In conclusion, the use of natural bioactive substances in hydrogel formulation would greatly reduce dependence on chemical pesticides and hence decreasing the environmental pollution and eventual harmful effects on plant, animal and human health.
{"title":"Biological investigations on the role of hydrogel formulations containing bioactive natural agents against some common phytopathogens of Phaseolus vulgaris L. and seed germination","authors":"H. S. Elshafie, M. Nuzzaci, G. Logozzo, Tania Gioia, I. Camele","doi":"10.4081/jbr.2020.9219","DOIUrl":"https://doi.org/10.4081/jbr.2020.9219","url":null,"abstract":"Recent scientific research have manipulated the use of hydrogel in seed coating technology based on synthetic and chemical additives. The current study has been carried out to evaluate the biological activity of new seed coating formulations containing hydrogel based on natural substances or beneficial microorganisms on seed germination and controlling some common diseases of Phaseolus vulgaris L. New formulations have been prepared as single mixtures of hydrogel with the following bioactive substances: i) oregano Essential Oil (org EO); ii) Ornithine Lipid (OL); and two microorganisms i) Burkholderia gladioli and ii) Trichoderma harzianum T22. Results revealed that, the hydrogel formulation based org EO showed the highest significant activity against the majority of tested phytopathogens in a dose dependent manner. Regarding the antagonistic microbial activity, results showed that hydrogel formulations based T. harzianum T22 and B. gladioli were able to significantly reduce the growth of the majority of tested phytopathogens. In addition, the highest significant percentage of seed germination has been achieved using the formulations of B. gladioli and org EO. Regarding the disease incidence suppression assay, results explicated that org EO and OL were able to significantly inhibit the fungal disease incidence on P. vulgaris seeds steadily depending on the tested concentrations. In conclusion, the use of natural bioactive substances in hydrogel formulation would greatly reduce dependence on chemical pesticides and hence decreasing the environmental pollution and eventual harmful effects on plant, animal and human health.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81815818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Kasim, M. Hatta, R. Natzir, V. Hadju, Y. Hala, Budu, G. Alam, S. As'ad, Ami Febriza, H. Idrus
Lime (Citrus aurantifolia) is a traditional plant that is widely used as antibacterial. This study proves the effect of Lime Peel Extract (LPE) on the colonization and growth, mediated by the activity of IL-6, of bacteria Salmonella typhi in Balb/c mice. Mice were divided into four groups; LPE 510 mg/kg body weight (bw), LPE 750 mg/kgbw, and positive and negative control. The examination was carried out 3 times, on the 5th day before the intervention, on the 10th day after the intervention and on the 30th day after maintenance. Intervention of LPE for 5 days can decrease the number of S. typhi colonies, even maintenance for 20 days after the intervention showed no bacterial growth. IL-6 pro-inflammatory cytokine activity increased on examination day 5 after S. typhi injection and decreased after intervention on day 10, it was significantly different between pre and post at all groups except for negative controls (p=0.15). The speed of decrease in IL-6 levels was the greatest at the LPE 750 mg/kgbw (velocity=-5.64%). LPE decreased serum levels of IL-6 and inhibited the growth of S. typhi colony in Balb/c mice. LPE has potential for antibacterial and anti-inflammatory.
{"title":"Antibacterial and anti-inflammatory effects of lime (Citrus aurantifolia) peel extract in Balb/c mice infected by Salmonella typhi","authors":"V. Kasim, M. Hatta, R. Natzir, V. Hadju, Y. Hala, Budu, G. Alam, S. As'ad, Ami Febriza, H. Idrus","doi":"10.4081/jbr.2020.8951","DOIUrl":"https://doi.org/10.4081/jbr.2020.8951","url":null,"abstract":"Lime (Citrus aurantifolia) is a traditional plant that is widely used as antibacterial. This study proves the effect of Lime Peel Extract (LPE) on the colonization and growth, mediated by the activity of IL-6, of bacteria Salmonella typhi in Balb/c mice. Mice were divided into four groups; LPE 510 mg/kg body weight (bw), LPE 750 mg/kgbw, and positive and negative control. The examination was carried out 3 times, on the 5th day before the intervention, on the 10th day after the intervention and on the 30th day after maintenance. Intervention of LPE for 5 days can decrease the number of S. typhi colonies, even maintenance for 20 days after the intervention showed no bacterial growth. IL-6 pro-inflammatory cytokine activity increased on examination day 5 after S. typhi injection and decreased after intervention on day 10, it was significantly different between pre and post at all groups except for negative controls (p=0.15). The speed of decrease in IL-6 levels was the greatest at the LPE 750 mg/kgbw (velocity=-5.64%). LPE decreased serum levels of IL-6 and inhibited the growth of S. typhi colony in Balb/c mice. LPE has potential for antibacterial and anti-inflammatory.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88121698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Preeclampsia (PE) is a multisystem pregnancy disease inflecting maternal, fetal mortality and morbidity. This case-control study aims to determine the biochemical changes in lipid profile, hepatic and renal functions among sixty preeclamptic women compared to sixty age-matching normotensive pregnant women from the Maternity Hospital in Sétif, Algeria. Several biomarkers were collected and have been determined by appropriate kits. Association between various parameters was evaluated using Pearson’s correlation coefficient, with significance at P<0.05. The PE women showed a significant increase in serum aminotransferase, gamma-glutamyl-transferase, alkaline phosphatase, as well as total cholesterol, triglycerides, urea, uric acid, and creatinine levels. However, a significant decrease in platelets, total protein, albumin, and plasma sodium levels were found in PE group. A positive correlation between aminotransferases and renal biomarkers was observed.PE has deleterious effects on liver and renal functions, and could be predicted by evaluating hepatic and renal biomarkers as well as lipid profile in the early stage of disease, for targeted prevention strategies in our population. Further studies on genetic assessment in cases of PE are needed for a better understanding of this disease’s pathophysiology in Algeria.
{"title":"Examination of hepato-renal functions and lipid panel among pregnant women with preeclampsia in Sétif, Algeria","authors":"R. Bourouba","doi":"10.4081/jbr.0.9035","DOIUrl":"https://doi.org/10.4081/jbr.0.9035","url":null,"abstract":"Preeclampsia (PE) is a multisystem pregnancy disease inflecting maternal, fetal mortality and morbidity. This case-control study aims to determine the biochemical changes in lipid profile, hepatic and renal functions among sixty preeclamptic women compared to sixty age-matching normotensive pregnant women from the Maternity Hospital in Sétif, Algeria. Several biomarkers were collected and have been determined by appropriate kits. Association between various parameters was evaluated using Pearson’s correlation coefficient, with significance at P<0.05. The PE women showed a significant increase in serum aminotransferase, gamma-glutamyl-transferase, alkaline phosphatase, as well as total cholesterol, triglycerides, urea, uric acid, and creatinine levels. However, a significant decrease in platelets, total protein, albumin, and plasma sodium levels were found in PE group. A positive correlation between aminotransferases and renal biomarkers was observed.PE has deleterious effects on liver and renal functions, and could be predicted by evaluating hepatic and renal biomarkers as well as lipid profile in the early stage of disease, for targeted prevention strategies in our population. Further studies on genetic assessment in cases of PE are needed for a better understanding of this disease’s pathophysiology in Algeria.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85674282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of proteases makes it possible to obtain partially hydrolyzed forms of macromolecules with unique properties. The importance of proteases for studying the structure and functions of fibrinogen forces scientists to search for new sources of highly specific proteases. Thus, the aim of this work was to study the content of the Pleurotus ostreatus culture fluid in search of fibrinogen- specific proteases. P. ostreatus was cultured for 14 days at 27°C. The culture fluid was collected and the protein fraction was salted out with NaCl and then dialyzed. Fibrinogen hydrolysis products by P. ostreatus protease were characterized using SDS PAGE under reducing conditions followed by immunoprobing using murine monoclonal antibodies I-5A (anti-Aα505-610) and 2d2a (anti-Bβ26-42). The study of turbidity and platelet aggregation was performed using a Multiskan FC spectrophotometric microplate reader and a SOLAR-2110 aggregometer, respectively. Electron microscopy of fibrils formed by truncated compared with native fibrins was performed using a transmission electron microscope N-600. Analysis of the products of fibrinogen hydrolysis with a fungal protease using SDS-PAGE demonstrated the cleavage of the alpha chain of fibrinogen exclusively with the formation of a truncated form of fibrinogen in which there are no C-terminal portions of αC regions with a molecular weight of 25 kDa. A study of turbidity showed that the polymerization of truncated fibrin is significantly impaired. The rate of lateral association of protofibrils significantly decreased from 1.5 to 2.2 times in the case of truncated fibrinogen compared to the native one depending on the initial concentration of fibrinogen. It was shown that platelet aggregation in the presence of fibrinogen without 25 kDa fragments of αC regions was less effective than in the presence of native fibrinogen. Application of the preparation of the fungal protease allows us to obtain high molecular forms of the fibrinogen molecule with cleaved 25 kDa peptides, which provide new information on the role of these peptides in the fibrinogen functioning.
{"title":"Fibrinogenolytic activity of protease from the culture fluid of Pleurotus ostreatus","authors":"S. Ye, Chernyshenko, Kucheriavyi Ye","doi":"10.4081/jbr.2020.9006","DOIUrl":"https://doi.org/10.4081/jbr.2020.9006","url":null,"abstract":"The use of proteases makes it possible to obtain partially hydrolyzed forms of macromolecules with unique properties. The importance of proteases for studying the structure and functions of fibrinogen forces scientists to search for new sources of highly specific proteases. Thus, the aim of this work was to study the content of the Pleurotus ostreatus culture fluid in search of fibrinogen- specific proteases. P. ostreatus was cultured for 14 days at 27°C. The culture fluid was collected and the protein fraction was salted out with NaCl and then dialyzed. Fibrinogen hydrolysis products by P. ostreatus protease were characterized using SDS PAGE under reducing conditions followed by immunoprobing using murine monoclonal antibodies I-5A (anti-Aα505-610) and 2d2a (anti-Bβ26-42). The study of turbidity and platelet aggregation was performed using a Multiskan FC spectrophotometric microplate reader and a SOLAR-2110 aggregometer, respectively. Electron microscopy of fibrils formed by truncated compared with native fibrins was performed using a transmission electron microscope N-600. \u0000Analysis of the products of fibrinogen hydrolysis with a fungal protease using SDS-PAGE demonstrated the cleavage of the alpha chain of fibrinogen exclusively with the formation of a truncated form of fibrinogen in which there are no C-terminal portions of αC regions with a molecular weight of 25 kDa. A study of turbidity showed that the polymerization of truncated fibrin is significantly impaired. The rate of lateral association of protofibrils significantly decreased from 1.5 to 2.2 times in the case of truncated fibrinogen compared to the native one depending on the initial concentration of fibrinogen. It was shown that platelet aggregation in the presence of fibrinogen without 25 kDa fragments of αC regions was less effective than in the presence of native fibrinogen. Application of the preparation of the fungal protease allows us to obtain high molecular forms of the fibrinogen molecule with cleaved 25 kDa peptides, which provide new information on the role of these peptides in the fibrinogen functioning.","PeriodicalId":9116,"journal":{"name":"Bollettino della Societa italiana di biologia sperimentale","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81399713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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