Syamsu Nur, F. Sami, M. Marwati, Nursamsiar Nursamsiar, A. Fadri, Khairuddin Khairuddin
The black mulberry (Morus nigra) plant has been widely developed for its bioactivity as natural medicine. This study evaluated plant extracts' total phenolic and flavonoid content and their correlation to M. nigra stem's antioxidant activity and toxicity. Dry powder from the M. nigra stem was extracted by maceration with 96% ethanol to obtain a thick extract (TE) and fractionated using hexane (HF), ethyl acetate (EAF), and ethanol-aqueous (EF). The samples were analyzed for a compound using reagents and the determination of total phenolic and flavonoid content. The samples were evaluated for antioxidant activity using several parameters and their cytotoxic effects using the BSLT method. Identification of compounds in EAF was confirmed to contain phenolic, flavonoids, alkaloids, saponins, tannins, steroids, and terpenoids. EAF showed higher phenolic and flavonoid content than others. The evaluation of antioxidant activity showed that extracts and fractions from M. nigra stems showed the ability to reduce ions and free radicals. EF sample has activity in reducing Mo (IV) ion by TAC method of 98.82±0.53 µM/mg, indicating substantial antioxidant capacity. In addition, EAF samples showed potential activity in reducing DPPH, hydroxyl, and peroxide radicals in the β-carotene bleaching method with IC50 values of 12.13, 42.06, and 57.6 µg/mL, respectively. Similar activity was also seen in the cytotoxic effect of a robust EAF sample with an LC50 value of 16.31 µg/mL. The results show that EAF can be developed as a raw material for traditional medicine as an antioxidant and anticancer candidate with a significant flavonoid and phenolics content.
{"title":"Phenolic and Flavonoid Content of Black Mulberry (Morus nigra L.) Stem and Their Evaluation Antioxidant and Cytotoxic Profile","authors":"Syamsu Nur, F. Sami, M. Marwati, Nursamsiar Nursamsiar, A. Fadri, Khairuddin Khairuddin","doi":"10.33084/bjop.v5i4.3760","DOIUrl":"https://doi.org/10.33084/bjop.v5i4.3760","url":null,"abstract":"The black mulberry (Morus nigra) plant has been widely developed for its bioactivity as natural medicine. This study evaluated plant extracts' total phenolic and flavonoid content and their correlation to M. nigra stem's antioxidant activity and toxicity. Dry powder from the M. nigra stem was extracted by maceration with 96% ethanol to obtain a thick extract (TE) and fractionated using hexane (HF), ethyl acetate (EAF), and ethanol-aqueous (EF). The samples were analyzed for a compound using reagents and the determination of total phenolic and flavonoid content. The samples were evaluated for antioxidant activity using several parameters and their cytotoxic effects using the BSLT method. Identification of compounds in EAF was confirmed to contain phenolic, flavonoids, alkaloids, saponins, tannins, steroids, and terpenoids. EAF showed higher phenolic and flavonoid content than others. The evaluation of antioxidant activity showed that extracts and fractions from M. nigra stems showed the ability to reduce ions and free radicals. EF sample has activity in reducing Mo (IV) ion by TAC method of 98.82±0.53 µM/mg, indicating substantial antioxidant capacity. In addition, EAF samples showed potential activity in reducing DPPH, hydroxyl, and peroxide radicals in the β-carotene bleaching method with IC50 values of 12.13, 42.06, and 57.6 µg/mL, respectively. Similar activity was also seen in the cytotoxic effect of a robust EAF sample with an LC50 value of 16.31 µg/mL. The results show that EAF can be developed as a raw material for traditional medicine as an antioxidant and anticancer candidate with a significant flavonoid and phenolics content.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85577726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Aliero, Felicia Yakubu Turba, A. Bagudo, Abdulazeez Aishat Folake, S. Manga
Consuming fruit contaminated with bacteria remains a crucial route of foodborne infection in developing countries and creates a severe public health burden. The research aimed to determine the antibiotic-resistant pattern of bacteria associated with the spoilage of Avocado pear (Persea americana) sold in the Sokoto metropolis. Twenty spoilt avocado fruits were obtained from market three in Sokoto Metropolis. The bacteriological analysis was carried out using the pour plate method. The antibiotic resistance pattern was determined using the Kirby-Bauer disk diffusion method. A total of five species of bacteria were isolated and identified in this study. The mean and standard error of total viable bacterial counts of avocado samples across three different locations ranged from 4.20±5.77 to 8.43±33.49 (x 104CFU/g). A total of 19 bacterial isolates were identified from avocado samples. Staphylococcus aureus had the highest frequency of occurrence, 16 (29.2%), while Klebsiella sp. had the lowest frequency of occurrence, 7 (12.7%). Among all antibiotics tested against bacterial species, Escherichia coli isolates were found to have 10 (91%) and 9 (82%) resistance against ciprofloxacin and streptomycin, respectively. Pseudomonas sp. was found to be 100% resistant to chloramphenicol and Septrin (cotrimoxazole). However, S. aureus was found to have 12 (72%) resistance to pefloxacin. Similarly, Klebsiella spp. were found to be 7 (100%) resistant to Septrin. Isolation of these bacterial species, especially E. coli from avocado samples analyzed, is of public health significance, especially the presence of antibiotic resistance species.
{"title":"Antibiotics Resistant Pattern of Bacteria Isolated from Spoiled Avocado Fruit Sold in Sokoto Metropolis","authors":"A. Aliero, Felicia Yakubu Turba, A. Bagudo, Abdulazeez Aishat Folake, S. Manga","doi":"10.33084/bjop.v5i4.3405","DOIUrl":"https://doi.org/10.33084/bjop.v5i4.3405","url":null,"abstract":"Consuming fruit contaminated with bacteria remains a crucial route of foodborne infection in developing countries and creates a severe public health burden. The research aimed to determine the antibiotic-resistant pattern of bacteria associated with the spoilage of Avocado pear (Persea americana) sold in the Sokoto metropolis. Twenty spoilt avocado fruits were obtained from market three in Sokoto Metropolis. The bacteriological analysis was carried out using the pour plate method. The antibiotic resistance pattern was determined using the Kirby-Bauer disk diffusion method. A total of five species of bacteria were isolated and identified in this study. The mean and standard error of total viable bacterial counts of avocado samples across three different locations ranged from 4.20±5.77 to 8.43±33.49 (x 104CFU/g). A total of 19 bacterial isolates were identified from avocado samples. Staphylococcus aureus had the highest frequency of occurrence, 16 (29.2%), while Klebsiella sp. had the lowest frequency of occurrence, 7 (12.7%). Among all antibiotics tested against bacterial species, Escherichia coli isolates were found to have 10 (91%) and 9 (82%) resistance against ciprofloxacin and streptomycin, respectively. Pseudomonas sp. was found to be 100% resistant to chloramphenicol and Septrin (cotrimoxazole). However, S. aureus was found to have 12 (72%) resistance to pefloxacin. Similarly, Klebsiella spp. were found to be 7 (100%) resistant to Septrin. Isolation of these bacterial species, especially E. coli from avocado samples analyzed, is of public health significance, especially the presence of antibiotic resistance species.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91361399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ethanol extract of Pandanus amaryllifolius leaves contains quercetin has anti-inflammatory properties. The gel dosage form is very appropriate for wound healing therapy because it gives a cold sensation to the skin. This study aims to formulate a P. amaryllifolius gel by variation of a gelling agent to compare the effect of a natural gelling agent, semi-synthetic gelling agent, and synthetic gelling agent on the physical characteristics of the gel. The gel formulation was made in three formulas. Evaluations include organoleptic, homogeneity, spreadability, adhesion, pH, and viscosity. The optimum gel formula was tested for release study using a dissolution apparatus 5. Determination of release kinetics model using the kinetics approach of zero-order, first-order, Higuchi, and Korsmeyer-Peppas. The organoleptic shows the gel's dark green color, P. amaryllifolius aromas, and good homogeneity. The gel formulas had pH values that matched the physical quality criteria of the gel. Data obtained for the spreadability test for F1, F2, and F3 were 12.16, 14.66, and 10.75 g.cm/sec, respectively. The F1, F2, and F3 adhesion test results were 11.66, 10, and 47.33, respectively. The viscosity of F1 was 15750 cps, F2 was 4807 cps, and F3 was 19380 cps. This study concludes that the optimum formula is obtained from the gelling agent of Na-CMC as a gelling agent. The number of quercetins released from F2 was 42.76%, and the release kinetics model followed the kinetics of Higuchi.
{"title":"Formulation and Evaluation of Natural Gel Containing Ethanolic Extract of Pandanus amaryllifolius R. Using Various Gelling Agents","authors":"Dyera Forestryana, Annisa Hayati, Aristha Novyra Putri","doi":"10.33084/bjop.v5i4.1411","DOIUrl":"https://doi.org/10.33084/bjop.v5i4.1411","url":null,"abstract":"Ethanol extract of Pandanus amaryllifolius leaves contains quercetin has anti-inflammatory properties. The gel dosage form is very appropriate for wound healing therapy because it gives a cold sensation to the skin. This study aims to formulate a P. amaryllifolius gel by variation of a gelling agent to compare the effect of a natural gelling agent, semi-synthetic gelling agent, and synthetic gelling agent on the physical characteristics of the gel. The gel formulation was made in three formulas. Evaluations include organoleptic, homogeneity, spreadability, adhesion, pH, and viscosity. The optimum gel formula was tested for release study using a dissolution apparatus 5. Determination of release kinetics model using the kinetics approach of zero-order, first-order, Higuchi, and Korsmeyer-Peppas. The organoleptic shows the gel's dark green color, P. amaryllifolius aromas, and good homogeneity. The gel formulas had pH values that matched the physical quality criteria of the gel. Data obtained for the spreadability test for F1, F2, and F3 were 12.16, 14.66, and 10.75 g.cm/sec, respectively. The F1, F2, and F3 adhesion test results were 11.66, 10, and 47.33, respectively. The viscosity of F1 was 15750 cps, F2 was 4807 cps, and F3 was 19380 cps. This study concludes that the optimum formula is obtained from the gelling agent of Na-CMC as a gelling agent. The number of quercetins released from F2 was 42.76%, and the release kinetics model followed the kinetics of Higuchi.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86455878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Burhan Ma’arif, F. A. Muslikh, Dilla Amalia, Anisah Mahardiani, Luthfi Achmad Muchlasi, Pramudita Riwanti, Maximus M. Taek, H. Laswati, M. Agil
This study was aimed to evaluate the metabolite contents and antineuroinflammatory potential of Marsilea crenata Presl. grown under a controlled environmental condition. The antineuroinflammatory test has been carried out in vitro using ethanolic extract of M. crenata leaves on HMC3 microglia cells. An in silico approach was applied to predict the active compounds of the extract. The HMC3 microglia cells were induced with IFNγ to create prolonged inflammatory conditions and then treated with 96% ethanolic extract of the M. crenata leaves of 62.5, 125, and 250 μg/mL. The expression of MHC II was analyzed using the ICC method with the CLSM instrument. Metabolites of the extract were profiled using UPLC-QToF-MS/MS instrument and MassLynx 4.1 software. In silico evaluation was conducted with molecular docking on 3OLS protein using PyRx 0.8 software, and physicochemical properties of the compounds were analyzed using SwissADME webtool. The ethanolic extract of M. crenata leaves could reduce the MHC II expression in HMC3 microglia cells in all concentrations with the values 97.458, 139.574, and 82.128 AU. The result of metabolite profiling found 79 compounds in the extract. In silico evaluation showed that 19 compounds gave agonist interaction toward 3OLS, and three met all parameters of physicochemical analysis. The ethanolic extract of the environmental-controlled growth of M. crenata leaves antineuroinflammatory activity on HMC3 microglia cells. The extract was predicted to contain some phytoestrogen compounds which act as 3OLS agonists.
{"title":"Metabolite Profiling of the Environmental-Controlled Growth of Marsilea crenata Presl. and Its In Vitro and In Silico Antineuroinflammatory Properties","authors":"Burhan Ma’arif, F. A. Muslikh, Dilla Amalia, Anisah Mahardiani, Luthfi Achmad Muchlasi, Pramudita Riwanti, Maximus M. Taek, H. Laswati, M. Agil","doi":"10.33084/bjop.v5i3.3262","DOIUrl":"https://doi.org/10.33084/bjop.v5i3.3262","url":null,"abstract":"This study was aimed to evaluate the metabolite contents and antineuroinflammatory potential of Marsilea crenata Presl. grown under a controlled environmental condition. The antineuroinflammatory test has been carried out in vitro using ethanolic extract of M. crenata leaves on HMC3 microglia cells. An in silico approach was applied to predict the active compounds of the extract. The HMC3 microglia cells were induced with IFNγ to create prolonged inflammatory conditions and then treated with 96% ethanolic extract of the M. crenata leaves of 62.5, 125, and 250 μg/mL. The expression of MHC II was analyzed using the ICC method with the CLSM instrument. Metabolites of the extract were profiled using UPLC-QToF-MS/MS instrument and MassLynx 4.1 software. In silico evaluation was conducted with molecular docking on 3OLS protein using PyRx 0.8 software, and physicochemical properties of the compounds were analyzed using SwissADME webtool. The ethanolic extract of M. crenata leaves could reduce the MHC II expression in HMC3 microglia cells in all concentrations with the values 97.458, 139.574, and 82.128 AU. The result of metabolite profiling found 79 compounds in the extract. In silico evaluation showed that 19 compounds gave agonist interaction toward 3OLS, and three met all parameters of physicochemical analysis. The ethanolic extract of the environmental-controlled growth of M. crenata leaves antineuroinflammatory activity on HMC3 microglia cells. The extract was predicted to contain some phytoestrogen compounds which act as 3OLS agonists.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85029488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayu Nala El Muna Haerussana, Angreni Ayuhastuti, Siti Fira Yuniar, Hana Alifah Bustami, Widyastiwi Widyastiwi
Dental caries was the most common disease in both adults and children. Streptococcus mutans is the main bacteria causing plaque formation and was the initiator of dental caries. Antibacterials derived from plants can be used to prevent plaque formation. Taro (Colosia esculenta) has been used in traditional medicine. Antibacterial compounds have been discovered in C. esculenta leaves. This study aimed to determine the ability of C. esculenta leaf ethanol extract to inhibit the growth of S. mutans ATCC 31987. Simplicia preparation, extract preparation, and phytochemical screening was carried out. Then, the antibacterial activity test was performed using the disc diffusion method to determine the zone of inhibition at various concentrations of 10, 20, 30, 40, 50, 60, and 70%. Colosia esculenta leaf ethanol extract contains alkaloids, flavonoids, triterpenoids, saponins, and produces an inhibition zone at each concentration variation. Very strong antibacterial activity was produced at a concentration of 70% at 21.11±0.46 mm, which was higher than the positive control.
{"title":"Taro (Colosia esculenta) Leaves Extract Inhibits Streptococcus mutans ATCC 31987","authors":"Ayu Nala El Muna Haerussana, Angreni Ayuhastuti, Siti Fira Yuniar, Hana Alifah Bustami, Widyastiwi Widyastiwi","doi":"10.33084/bjop.v5i3.3156","DOIUrl":"https://doi.org/10.33084/bjop.v5i3.3156","url":null,"abstract":"Dental caries was the most common disease in both adults and children. Streptococcus mutans is the main bacteria causing plaque formation and was the initiator of dental caries. Antibacterials derived from plants can be used to prevent plaque formation. Taro (Colosia esculenta) has been used in traditional medicine. Antibacterial compounds have been discovered in C. esculenta leaves. This study aimed to determine the ability of C. esculenta leaf ethanol extract to inhibit the growth of S. mutans ATCC 31987. Simplicia preparation, extract preparation, and phytochemical screening was carried out. Then, the antibacterial activity test was performed using the disc diffusion method to determine the zone of inhibition at various concentrations of 10, 20, 30, 40, 50, 60, and 70%. Colosia esculenta leaf ethanol extract contains alkaloids, flavonoids, triterpenoids, saponins, and produces an inhibition zone at each concentration variation. Very strong antibacterial activity was produced at a concentration of 70% at 21.11±0.46 mm, which was higher than the positive control.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85623764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Harry Noviardi, D. Iswantini, S. Mulijani, S. T. Wahyudi, T. Khusniati
Inflammatory diseases are diseases characterized by inflammatory symptoms. Acute inflammatory disease can cause dysregulation of the inflammatory immune response, thereby inhibiting the development of protective immunity against infection. Among the acute inflammatory disease is COVID-19. The initial viral infection causes the antigen-presenting cells to detect the virus through a phagocytosis mechanism in the form of macrophage and dendritic cells. Lactobacillus fermentum and L. plantarum are gram-positive bacteria potentially serving as immunomodulators caused by inflammation and immune system response. Short-chain fatty acids (SCFA) produced by Lactobacillus can induce immune response through tolerogenic dendritic cells. This probiotic bacterium can induce the production of different cytokines or chemokines. Following the results of in vitro and in vivo tests, L. fermentum and L. plantarum can induce IL-10 release to activate regulatory T-cell and inhibit tumor necrosis factor-α (TNF-α) binding activity of nuclear factor kappa B (NF-κB). Literature review showed that dysregulation of inflammatory immune response disorders due to inflammatory disease could be treated using probiotic bacteria L. fermentum and L. plantarum. Therefore, it is necessary to conduct further studies on the potential of indigenous Indonesian strains of these two bacteria as anti-inflammatory and immunostimulants.
{"title":"Anti-inflammatory and Immunostimulant Therapy with Lactobacillus fermentum and Lactobacillus plantarum in COVID-19: A Literature Review","authors":"Harry Noviardi, D. Iswantini, S. Mulijani, S. T. Wahyudi, T. Khusniati","doi":"10.33084/bjop.v5i3.3367","DOIUrl":"https://doi.org/10.33084/bjop.v5i3.3367","url":null,"abstract":"Inflammatory diseases are diseases characterized by inflammatory symptoms. Acute inflammatory disease can cause dysregulation of the inflammatory immune response, thereby inhibiting the development of protective immunity against infection. Among the acute inflammatory disease is COVID-19. The initial viral infection causes the antigen-presenting cells to detect the virus through a phagocytosis mechanism in the form of macrophage and dendritic cells. Lactobacillus fermentum and L. plantarum are gram-positive bacteria potentially serving as immunomodulators caused by inflammation and immune system response. Short-chain fatty acids (SCFA) produced by Lactobacillus can induce immune response through tolerogenic dendritic cells. This probiotic bacterium can induce the production of different cytokines or chemokines. Following the results of in vitro and in vivo tests, L. fermentum and L. plantarum can induce IL-10 release to activate regulatory T-cell and inhibit tumor necrosis factor-α (TNF-α) binding activity of nuclear factor kappa B (NF-κB). Literature review showed that dysregulation of inflammatory immune response disorders due to inflammatory disease could be treated using probiotic bacteria L. fermentum and L. plantarum. Therefore, it is necessary to conduct further studies on the potential of indigenous Indonesian strains of these two bacteria as anti-inflammatory and immunostimulants.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76562655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Uncaria cordata (Lour). Merr (akar kaik-kaik) is one of the medicinal plants used as antibacterial because it contains bioactive compounds that can inhibit the growth of microorganisms. The plant is one of the sources of endophyte fungal isolates that can be developed as an alternative to producing antibacterial compounds. This research aimed to isolate the endophytic fungus from the leaves of U. cordata and know the antibacterial activity against Propionibacterium acnes and Escherichia coli by disc diffusion. The Fungi that were isolated from the leaves of U. cordata were 17 isolates. The isolates were continued for antibacterial activity testing: IFED 1 (Nigrospora sp.), IFED 2 (Aspergillus sp.), IFED 3 (Fusarium sp.), and IFED 4, whose genus was unknown. The results obtained were fungal isolates IFED 1 to IFED 4 had activity in inhibiting the growth of P. acnes with moderate category (18.16 mm) and weak categories (6.21, 6.16, and 6.68 mm) and in E. coli with moderate category (14.56 mm) and weak categories (6.53, 6.71, and 7.23 mm). The results of One-Way ANOVA and Tukey's test showed a significant difference (p <0.05) between the diameter of the inhibition zone with the type of endophytic fungus supernatant isolated from the leaves of U. cordata. The best isolate of endophytic fungi inhibiting P. acnes and E. coli bacteria was IFED 1 (Nigrospora sp).
{"title":"Isolation of Endophytic Fungus from Leaves of Uncaria cordata (Lour.) Merr and Antibacterial Activity Against Propionibacterium acnes and Escherichia coli","authors":"Melzi Octaviani, Winda Yusma Ameliah, N. Frimayanti, Meiriza Djohari, Haiyul Fadhli","doi":"10.33084/bjop.v5i3.3692","DOIUrl":"https://doi.org/10.33084/bjop.v5i3.3692","url":null,"abstract":"Uncaria cordata (Lour). Merr (akar kaik-kaik) is one of the medicinal plants used as antibacterial because it contains bioactive compounds that can inhibit the growth of microorganisms. The plant is one of the sources of endophyte fungal isolates that can be developed as an alternative to producing antibacterial compounds. This research aimed to isolate the endophytic fungus from the leaves of U. cordata and know the antibacterial activity against Propionibacterium acnes and Escherichia coli by disc diffusion. The Fungi that were isolated from the leaves of U. cordata were 17 isolates. The isolates were continued for antibacterial activity testing: IFED 1 (Nigrospora sp.), IFED 2 (Aspergillus sp.), IFED 3 (Fusarium sp.), and IFED 4, whose genus was unknown. The results obtained were fungal isolates IFED 1 to IFED 4 had activity in inhibiting the growth of P. acnes with moderate category (18.16 mm) and weak categories (6.21, 6.16, and 6.68 mm) and in E. coli with moderate category (14.56 mm) and weak categories (6.53, 6.71, and 7.23 mm). The results of One-Way ANOVA and Tukey's test showed a significant difference (p <0.05) between the diameter of the inhibition zone with the type of endophytic fungus supernatant isolated from the leaves of U. cordata. The best isolate of endophytic fungi inhibiting P. acnes and E. coli bacteria was IFED 1 (Nigrospora sp).","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77594542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Implementation of pharmacists services provided in all pharmacies must meet the standard of pharmaceutical services guidelines, including the provision of drug information. The study aims to compare the patient's perception of drug counseling services by pharmacy staff based on the type of pharmacy ownership (franchise/non-franchise) in Semarang. This research is an observational study with a cross-sectional design. Samples were taken of 286 respondents with a quota sampling technique. Data were collected using an online google form questionnaire tested for validity and reliability. The statistical analysis results used the Mann-Whitney test with a p-value of <0.05. There is no significant difference between the patient's perception of the drug information counseling services by pharmacy staff at the franchise or non-franchise pharmacies in Semarang with a p-value of 0.264. This study also found that the standard information given by pharmacy staff is healthy eating and education about antibiotics used in the common cold. In addition, only 55% of respondents were sure that the pharmacy staff who gave them drug information in pharmacies was a pharmacist. Consequently, we humbly recommend that pharmacists consistently wear their pharmacist identification.
{"title":"Comparison of Patient's Satisfaction with Pharmaceutical Care Services in Ownership-Based Pharmacies in Semarang, Indonesia","authors":"Nisa Febrinasari, A. Rosyid, Fadhila Huswatunnida","doi":"10.33084/bjop.v5i3.2158","DOIUrl":"https://doi.org/10.33084/bjop.v5i3.2158","url":null,"abstract":"Implementation of pharmacists services provided in all pharmacies must meet the standard of pharmaceutical services guidelines, including the provision of drug information. The study aims to compare the patient's perception of drug counseling services by pharmacy staff based on the type of pharmacy ownership (franchise/non-franchise) in Semarang. This research is an observational study with a cross-sectional design. Samples were taken of 286 respondents with a quota sampling technique. Data were collected using an online google form questionnaire tested for validity and reliability. The statistical analysis results used the Mann-Whitney test with a p-value of <0.05. There is no significant difference between the patient's perception of the drug information counseling services by pharmacy staff at the franchise or non-franchise pharmacies in Semarang with a p-value of 0.264. This study also found that the standard information given by pharmacy staff is healthy eating and education about antibiotics used in the common cold. In addition, only 55% of respondents were sure that the pharmacy staff who gave them drug information in pharmacies was a pharmacist. Consequently, we humbly recommend that pharmacists consistently wear their pharmacist identification.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74238453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assalamu’alaikum Wr. Wb. Alhamdulillahirabbil ‘alamin. The next edition of the Borneo Journal of Pharmacy (Borneo J Pharm), has been published at August 2022. This edition contains ten articles: Pharmacognosy-Phytochemistry, Pharmaceutical, Microbiology Pharmacy, Natural Product Development, Clinical-Community Pharmacy, and Management Pharmacy. This edition includes writings from four countries: Costa Rica, India, Indonesia, Netherlands, and Nigeria. The authors come from several institutions, including Universitas Ahmad Dahlan, K24 Indonesia, Institut Sains dan Teknologi Nasional, Universidad de Costa Rica, Kebbi State University of Science and Technology, Aliero, Federal University, Birnin Kebbi, Sekolah Tinggi Teknologi Industri dan Farmasi Bogor, IPB University, National Research and Innovation Agency Republic of Indonesia, Poltekkes Kemenkes Bandung, Sekolah Tinggi Ilmu Farmasi Riau, Universitas Islam Negeri Maulana Malik Ibrahim, Universitas Airlangga, Universitas Hang Tuah, Universitas Katolik Widya Mandira, University of Groningen, Universitas Gadjah Mada, Universitas Hasanuddin, Universitas Syiah Kuala, Poltekkes Kemenkes Maluku, and Universitas Islam Sultan Agung. Editorial boards are fully aware that there are still room for improvement in this edition, hence with all humility willing to accept constructive suggestions and feedback for improvements to the publication for the next editions. The editorial board would like to thank all editors and reviewers, and contributors of the scientific articles who have provided the repetoire in this issue. We hope that all parties, especially the contributors, could re-participate for the publication in the next edition on November 2022. Wassalamu’alaikum Wr. Wb.
{"title":"Cover, Content, and Editorial Note from Borneo J Pharm Vol. 5 No. 3 August 2022","authors":"C. E. O. Borneo J Pharm","doi":"10.33084/bjop.v5i3.4054","DOIUrl":"https://doi.org/10.33084/bjop.v5i3.4054","url":null,"abstract":"Assalamu’alaikum Wr. Wb. \u0000Alhamdulillahirabbil ‘alamin. The next edition of the Borneo Journal of Pharmacy (Borneo J Pharm), has been published at August 2022. This edition contains ten articles: Pharmacognosy-Phytochemistry, Pharmaceutical, Microbiology Pharmacy, Natural Product Development, Clinical-Community Pharmacy, and Management Pharmacy. This edition includes writings from four countries: Costa Rica, India, Indonesia, Netherlands, and Nigeria. The authors come from several institutions, including Universitas Ahmad Dahlan, K24 Indonesia, Institut Sains dan Teknologi Nasional, Universidad de Costa Rica, Kebbi State University of Science and Technology, Aliero, Federal University, Birnin Kebbi, Sekolah Tinggi Teknologi Industri dan Farmasi Bogor, IPB University, National Research and Innovation Agency Republic of Indonesia, Poltekkes Kemenkes Bandung, Sekolah Tinggi Ilmu Farmasi Riau, Universitas Islam Negeri Maulana Malik Ibrahim, Universitas Airlangga, Universitas Hang Tuah, Universitas Katolik Widya Mandira, University of Groningen, Universitas Gadjah Mada, Universitas Hasanuddin, Universitas Syiah Kuala, Poltekkes Kemenkes Maluku, and Universitas Islam Sultan Agung. \u0000Editorial boards are fully aware that there are still room for improvement in this edition, hence with all humility willing to accept constructive suggestions and feedback for improvements to the publication for the next editions. The editorial board would like to thank all editors and reviewers, and contributors of the scientific articles who have provided the repetoire in this issue. We hope that all parties, especially the contributors, could re-participate for the publication in the next edition on November 2022. \u0000Wassalamu’alaikum Wr. Wb.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73880190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catalina Ayón, D. Castan, Adrian J. Mora, Dunia Naranjo, Francini Obando, Juan José Mora
The eye is an organ that allows us to observe the outside world. Pathologies of the eye's posterior segment, such as glaucoma, macular degeneration, diabetic retinopathy, uveitis, and retinoblastoma, cause vision loss. Traditional treatments consist of applying topical medications that do not penetrate properly or using high doses that generate adverse effects. Different laser surgeries stop the pathology's progression but do not allow visual improvement. So, an alternative is to use monoclonal antibodies, proteins produced by different processes that selectively bind to metabolites associated with diseases, reducing the adverse effects of traditional treatments and improving the application of the drug in the area. The two main molecular targets are TNF (adalimumab, infliximab, and certolizumab pegol) and VEGF (bevacizumab and ranibizumab); other possibilities are under investigation.
{"title":"Monoclonal Antibodies: A Therapeutic Option for the Treatment of Ophthalmic Diseases of the Eye Posterior Segment","authors":"Catalina Ayón, D. Castan, Adrian J. Mora, Dunia Naranjo, Francini Obando, Juan José Mora","doi":"10.33084/bjop.v5i3.2095","DOIUrl":"https://doi.org/10.33084/bjop.v5i3.2095","url":null,"abstract":"The eye is an organ that allows us to observe the outside world. Pathologies of the eye's posterior segment, such as glaucoma, macular degeneration, diabetic retinopathy, uveitis, and retinoblastoma, cause vision loss. Traditional treatments consist of applying topical medications that do not penetrate properly or using high doses that generate adverse effects. Different laser surgeries stop the pathology's progression but do not allow visual improvement. So, an alternative is to use monoclonal antibodies, proteins produced by different processes that selectively bind to metabolites associated with diseases, reducing the adverse effects of traditional treatments and improving the application of the drug in the area. The two main molecular targets are TNF (adalimumab, infliximab, and certolizumab pegol) and VEGF (bevacizumab and ranibizumab); other possibilities are under investigation.","PeriodicalId":9118,"journal":{"name":"Borneo Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86167810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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