BMC Gastroenterology最新文献

Objectives: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease. This research investigates the role of miR-199b-5p in NAFLD development.

Method: This study enrolled 110 non-NAFLD patients and 90 NAFLD patients and collected their clinical data and serum samples. The level of miR-199b-5p was measured via qRT-PCR. Through experiments such as ELISA, biochemical assays, and CCK-8, the effects of miR-199b-5p on OA-induced inflammation, lipid metabolism, viability, and oxidative stress in HepG2 cells were analyzed. Meanwhile, the targeting relationship between miR-199b-5p and SIRT1 was verified through dual-luciferase assay and Western blot analysis.

Results: miR-199b-5p was highly expressed in the NAFLD group and was significantly correlated with BMI, WC, TG, LDL-C, and HDL-C (P < 0.0001). ROC analysis demonstrated its diagnostic potential for NAFLD (AUC = 0.875, P < 0.0001). The OA-induced HepG2 cell model revealed that the miR-199b-5p inhibitor alleviated OA-induced suppression of cell viability, suppressed inflammation, reduced lipid levels, and alleviated oxidative stress. Furthermore, SIRT1 levels in the NAFLD group were significantly decreased and showed a significant negative correlation with miR-199b-5p (P < 0.0001).

Conclusions: miR-199b-5p is significantly elevated in the serum of NAFLD patients and shows a positive correlation with multiple metabolic indicators. This suggests its potential as a diagnostic biomarker for NAFLD. Additionally, inhibition of miR-199b-5p protects against OA-induced hepatocyte injury, partially through targeting SIRT1, which regulates inflammatory response and oxidative stress.

Background: Constipation and diarrhea are prevalent gastrointestinal health issues that significantly impact productivity and daily life, often leading to psychological problems and exacerbating public health burdens. Alcohol is a commonly consumed beverage worldwide, and previous studies suggest that it may exert certain effects on the gastrointestinal tract. Investigating the relationship between patterns of alcohol consumption and the presence of gastrointestinal symptoms such as constipation or diarrhea can aid in the formulation of public health strategies and improve management plans for alcohol use and gastrointestinal health.

Objective: To explore the relationship between patterns of alcohol consumption (average daily alcohol consumption and drinking levels) and the presence of gastrointestinal symptoms such as constipation or diarrhea.

Methods: The study utilized data from the National Health and Nutrition Examination Survey (NHANES) database from 2005 to 2010, enrolling a total of 12,959 eligible participants aged 20 years and older. Multivariate logistic regression analysis was employed to investigate the association of drinking patterns with constipation and diarrhea. Restricted cubic spline (RCS) curves were used to confirm the nonlinear relationship between these variables. Finally, subgroup analyses were conducted to identify high-risk groups and populations with specific effects.

Results: In this study, a negative correlation was observed between the average daily alcohol consumption and constipation (OR: 0.94, 95% CI: 0.91-0.97; P < 0.001). Similarly, a negative correlation was found between drinking levels and constipation (P < 0.001). These results remained stable even after adjusting for all confounding factors. However, the negative correlation between both average daily alcohol consumption and drinking levels with diarrhea was only present in model 1, and the results became unstable after adjusting for all confounding factors.

Conclusion:  Our study reveals a negative correlation between drinking patterns and both constipation and diarrhea. However, this association warrants further biological research for validation.

Objective: The aim of this study was to investigate the validity of DAOH-90 in a contemporary cohort of electively operated patients with colorectal cancer and explore its relationship with postoperative outcomes.

Design: The study was a retrospective, observational cohort study.

Settings: Data on patients operated from April 2014 to April 2019 were collected from the Danish Colorectal Cancer Group database and enriched by information from the Danish National Patient Register. DAOH-90 was analyzed by quantile regression for the 10th, 25th, 50th, 75th, and 90th percentile, using Mann-Whitney U-test, and association between DAOH-90 and 1-and 5-year mortality was analyzed using a Cox regression.

Patients: A total of 16,183 patients with colorectal cancer were included. Median DAOH-90 was 85 (IQR: 82-87). The 90-day mortality was 2.2%.

Main outcome measures: The primary outcome was the association between DAOH-90 quantiles and long-term mortality (1 and 5 years).

Results: There was significant association between DAOH-90 in the 25th, 50th and 75th quantile and death between 90 and 365 days (p = 0.0008-< 0.0001), and for death between 1 and 5 years (p = 0.0026-p < 0.0001). Both medical and surgical complications were associated with significantly lower DAOH-90 for all percentiles. The multivariable quantile regression confirmed that notably complications and poor performance status had great impact on DAOH-90.

Conclusion: DAOH-90 was associated with several demographic and comorbidity related variables, including severe surgical complications. DAOH-90 was also associated with long-term mortality.