Pub Date : 2016-01-01DOI: 10.4172/2161-0517.C1.015
H. Bouguerra
{"title":"Influenza severe cases and deaths in Tunisia: Season 2015-2016","authors":"H. Bouguerra","doi":"10.4172/2161-0517.C1.015","DOIUrl":"https://doi.org/10.4172/2161-0517.C1.015","url":null,"abstract":"","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"05 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70460914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-07DOI: 10.4172/2161-0517.1000E110
C. N. Kumar, P. Reddy, K. Ramalingam
Dr. CVM Naresh Kumar1*, P Sreenivasulu Reddy2 and K Ramalingam3 1Clinical Virology Lab, Central Clinical Laboratory, Narayana Super Speciality Hospital, Nellore, Andhra Pradesh, India 2Department of Microbiology, Narayana Medical College, Nellore, Andhra Pradesh, India 3Department of Biochemistry, Narayana Medical College and Super speciality Hospital, Nellore, Andhra Pradesh, India *Corresponding author: Dr. CVM Naresh Kumar, Clinical Virology Lab, Central Clinical Laboratory, Narayana Super Speciality Hospital, Nellore, Andhra Pradesh, India, Tel: +91 8106330817; E-mail: cvmnareshkumar@gmail.com Received date: Dec 01, 2015; Accepted date: Dec 3 2015; Published date: Dec 7, 2015
{"title":"MERS Outbreak: Is India Prepared?","authors":"C. N. Kumar, P. Reddy, K. Ramalingam","doi":"10.4172/2161-0517.1000E110","DOIUrl":"https://doi.org/10.4172/2161-0517.1000E110","url":null,"abstract":"Dr. CVM Naresh Kumar1*, P Sreenivasulu Reddy2 and K Ramalingam3 1Clinical Virology Lab, Central Clinical Laboratory, Narayana Super Speciality Hospital, Nellore, Andhra Pradesh, India 2Department of Microbiology, Narayana Medical College, Nellore, Andhra Pradesh, India 3Department of Biochemistry, Narayana Medical College and Super speciality Hospital, Nellore, Andhra Pradesh, India *Corresponding author: Dr. CVM Naresh Kumar, Clinical Virology Lab, Central Clinical Laboratory, Narayana Super Speciality Hospital, Nellore, Andhra Pradesh, India, Tel: +91 8106330817; E-mail: cvmnareshkumar@gmail.com Received date: Dec 01, 2015; Accepted date: Dec 3 2015; Published date: Dec 7, 2015","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2015-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70458618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-07DOI: 10.4172/2161-0517.1000148
Melese Yilma, M. Mekonnen
The objective of the present study is to assess the sero-prevalence and associated risk factors for small ruminants’ bluetongue infection in selected agro-ecology of woliyta zone, southern Ethiopia. Serum samples were collected randomly from the accessed small ruminates and screened for detection of BTV-specific immunoglobulin G (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 476 serum samples were tested and 196 (41.17%) were positive for bluetongue virus antibodies. A prevalence rate ranging from 26.53% for the midland altitude to 73.47% for the lowland was recorded. To disease associated risk factor age, and location was recorded using multivariate analysis of logistic regression model. Within species goat 114 (58.2%) and sheep 82 (41.8%) sero positive reactors was recorded. This is the first report indicating the presence of bluetongue virus infection in the area.
{"title":"Competitive Enzyme Linked Immuno-Sorbent Assay (c-ELISA) Based Sero-Prevalence of Bluetongue Virus (BTV) on Small Ruminants in SelectedAreas of Wolyita, Southern Ethiopia","authors":"Melese Yilma, M. Mekonnen","doi":"10.4172/2161-0517.1000148","DOIUrl":"https://doi.org/10.4172/2161-0517.1000148","url":null,"abstract":"The objective of the present study is to assess the sero-prevalence and associated risk factors for small ruminants’ bluetongue infection in selected agro-ecology of woliyta zone, southern Ethiopia. Serum samples were collected randomly from the accessed small ruminates and screened for detection of BTV-specific immunoglobulin G (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 476 serum samples were tested and 196 (41.17%) were positive for bluetongue virus antibodies. A prevalence rate ranging from 26.53% for the midland altitude to 73.47% for the lowland was recorded. To disease associated risk factor age, and location was recorded using multivariate analysis of logistic regression model. Within species goat 114 (58.2%) and sheep 82 (41.8%) sero positive reactors was recorded. This is the first report indicating the presence of bluetongue virus infection in the area.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2015-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70455680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-07DOI: 10.4172/2161-0517.1000149
Bikila Wedajo
Fungicides viz., curzate and sancozeb were used at different concentrations that is., 100, 200, 400, 600, 800 and 1000 ppm active ingredient to evaluate Trichoderma species viz., Trichoderma harzianum (AUT1) and Trichoderma viride (AUT2) in favour of tolerance to fungicides. By increasing the fungicide concentrations to 400 ppm (sancozeb) and 600 ppm (curzate), the Trichoderma species tolerate the fungicides 50% and slightly incompatible at higher concentrations of 800 and 1000 ppm, and completely inhibited beyond 1000 ppm compared to the control for both fungicides. The highest 97.8% was recorded at 100 ppm for curzate fungicides when combined with AUT2 and 96.7% of compatibility was recorded at concentration of 100 ppm when AUT1 is combined with the same fungicides. But, in the case of sancozeb the highest compatibility (97.8%) was recorded when combined AUT2, and 95.5% with AUT1 at 100 ppm. Therefore, the present compatibility study assist in the selection of bio control agents, which can be used with reduced am``ount of preferred fungicides for the control of plant pathogenic fungi.
{"title":"Compatibility Studies of Fungicides with Combination of TrichodermaSpecies under In vitro Conditions","authors":"Bikila Wedajo","doi":"10.4172/2161-0517.1000149","DOIUrl":"https://doi.org/10.4172/2161-0517.1000149","url":null,"abstract":"Fungicides viz., curzate and sancozeb were used at different concentrations that is., 100, 200, 400, 600, 800 and 1000 ppm active ingredient to evaluate Trichoderma species viz., Trichoderma harzianum (AUT1) and Trichoderma viride (AUT2) in favour of tolerance to fungicides. By increasing the fungicide concentrations to 400 ppm (sancozeb) and 600 ppm (curzate), the Trichoderma species tolerate the fungicides 50% and slightly incompatible at higher concentrations of 800 and 1000 ppm, and completely inhibited beyond 1000 ppm compared to the control for both fungicides. The highest 97.8% was recorded at 100 ppm for curzate fungicides when combined with AUT2 and 96.7% of compatibility was recorded at concentration of 100 ppm when AUT1 is combined with the same fungicides. But, in the case of sancozeb the highest compatibility (97.8%) was recorded when combined AUT2, and 95.5% with AUT1 at 100 ppm. Therefore, the present compatibility study assist in the selection of bio control agents, which can be used with reduced am``ount of preferred fungicides for the control of plant pathogenic fungi.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2015-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70455705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-12DOI: 10.4172/2161-0517.1000147
A. Andersson, H. B. Reineck, A. Nyman, P. Wallgren
Infections with Aleutian disease virus (ADV) cause a progressive hypergammaglobulinemia, immune complex formation and plasma cell infiltrations in internal organs which induce a multi-systemic disease with high mortality. Serum ADV antibodies have traditionally been diagnosed with counter immunoelectrophoresis (CIEP) as a gold standard for qualitative assessment separating infected from non-infected animals, but less laborious ELISA methods have been confirmed to be equally sensitive. A way to simplify the diagnostics further could be to demonstrate antibodies in Dried blood spot samples (DBS). However, quantitative analysis of ADV antibodies in DBS and its correlation to the degree of hypergammaglobulinemia have not been scientifically published. The aim of this paper was to describe the adaptation and validation of the VP2 ELISA to ADV antibody detection in DBS and compare the estimated antibody levels in DBS to CIEP results, the estimated antibody levels and albumin: gamma globulin ratio in serum. The VP2 ELISA worked technically well when transferred from serum to DBS with mean intra-assay and mean inter-assay coefficients of variation within ± 20%. The DBS VP2 ELISA had a sensitivity of 97.3% and specificity of 93.2% compared to CIEP. Further, we found a correlation coefficient between the level of antibodies in DBS and the A:γG ratio of -0.81. The correlation between the A:γG ratio and the OD450 value was superior in DBS compared to serum samples from the same mink with the most pronounced difference at low A:γG ratios. Our results confirmed that the VP2 ELISA could detect ADV antibodies in DBS with a high sensitivity and specificity when employing CIEP as gold standard. The antibody titers estimated with DBS VP2 ELISA were well correlated to the antibody titters and A:γG ratios in serum, and the DBS VP2 ELISA could be an applicable and preferable tool for estimating AD progression in mink.
{"title":"Quantitative Detection of Antibodies to Aleutian Disease Virus in DriedBlood Spots as an Estimation of Hypergammaglobulinemia in Mink","authors":"A. Andersson, H. B. Reineck, A. Nyman, P. Wallgren","doi":"10.4172/2161-0517.1000147","DOIUrl":"https://doi.org/10.4172/2161-0517.1000147","url":null,"abstract":"Infections with Aleutian disease virus (ADV) cause a progressive hypergammaglobulinemia, immune complex formation and plasma cell infiltrations in internal organs which induce a multi-systemic disease with high mortality. Serum ADV antibodies have traditionally been diagnosed with counter immunoelectrophoresis (CIEP) as a gold standard for qualitative assessment separating infected from non-infected animals, but less laborious ELISA methods have been confirmed to be equally sensitive. A way to simplify the diagnostics further could be to demonstrate antibodies in Dried blood spot samples (DBS). However, quantitative analysis of ADV antibodies in DBS and its correlation to the degree of hypergammaglobulinemia have not been scientifically published. The aim of this paper was to describe the adaptation and validation of the VP2 ELISA to ADV antibody detection in DBS and compare the estimated antibody levels in DBS to CIEP results, the estimated antibody levels and albumin: gamma globulin ratio in serum. The VP2 ELISA worked technically well when transferred from serum to DBS with mean intra-assay and mean inter-assay coefficients of variation within ± 20%. The DBS VP2 ELISA had a sensitivity of 97.3% and specificity of 93.2% compared to CIEP. Further, we found a correlation coefficient between the level of antibodies in DBS and the A:γG ratio of -0.81. The correlation between the A:γG ratio and the OD450 value was superior in DBS compared to serum samples from the same mink with the most pronounced difference at low A:γG ratios. Our results confirmed that the VP2 ELISA could detect ADV antibodies in DBS with a high sensitivity and specificity when employing CIEP as gold standard. The antibody titers estimated with DBS VP2 ELISA were well correlated to the antibody titters and A:γG ratios in serum, and the DBS VP2 ELISA could be an applicable and preferable tool for estimating AD progression in mink.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"5 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-0517.1000147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70456001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-23DOI: 10.4172/2161-0517.1000E109
G. Kotwal, S. Chien
The leading causes of death among the senior population are similar to those in the general population as a whole; however, several diseases have special features among the elderly. We have studied several people who have lived long lives (i.e., 95+ years), and here we share insights on one or two in particular and what we have learned from them.
{"title":"Infection Control is One Major Key to Longevity","authors":"G. Kotwal, S. Chien","doi":"10.4172/2161-0517.1000E109","DOIUrl":"https://doi.org/10.4172/2161-0517.1000E109","url":null,"abstract":"The leading causes of death among the senior population are similar to those in the general population as a whole; however, several diseases have special features among the elderly. We have studied several people who have lived long lives (i.e., 95+ years), and here we share insights on one or two in particular and what we have learned from them.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2015-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70458555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-09-29DOI: 10.4172/2161-0517.1000146
Matthew Osaro, Frank-Peterside, Okonko Io, E. Ughala, Obike-Martins
Three diagnostic rapid test kits (Chembio HIV-1/2 Stat-Pak, Alere DetermineTM HIV-1/2, and Core HIV-1/2) for human immunodeficiency virus types 1 and 2 (HIV-1/2) antibodies detection were evaluated against a gold standard fourth generation ELISA (Dia-Pro). Hundred and seventy two serum samples were collected from Lulubriggs Health Center, University of Port Harcourt, Rivers State. All samples were tested for HIV-1/2 using the three rapid test (parallel algorithm and serial algorithm), and retested using the 4th generation ELISA. The results obtained were compared with those from the ELISA. The DetermineTM rapid strips had sensitivity of 66.7%, specificity of 100%, positive predictive value 100%, negative predictive value 89.4% and total agreement of 91.3%. Stat-pak and Core HIV-1/2 rapid strips both had sensitivity of 71.7%, specificity of 100%, positive predictive value 100%, and negative predictive value 90.7% and total agreement of 92.4%. Determine was mostly used by the participant laboratories 18 of 30 (60%). The performance evaluation of the testing algorithm showed that parallel algorithm is more accurate in comparison to serial algorithm. The result of this study reveals that most rapid test are less sensitive and the accuracy of serial testing algorithm is low, this implies that most acute HIV infection will be missed with rapid assay and false negative results will be reported as actual negative with serial algorithm.
{"title":"Evaluation of Commonly Used Rapid Test Kits and Serological Serial TestingAlgorithm for Diagnosis of HIV-1 and 2 Antibodies in Port Harcourt, Nigeria","authors":"Matthew Osaro, Frank-Peterside, Okonko Io, E. Ughala, Obike-Martins","doi":"10.4172/2161-0517.1000146","DOIUrl":"https://doi.org/10.4172/2161-0517.1000146","url":null,"abstract":"Three diagnostic rapid test kits (Chembio HIV-1/2 Stat-Pak, Alere DetermineTM HIV-1/2, and Core HIV-1/2) for human immunodeficiency virus types 1 and 2 (HIV-1/2) antibodies detection were evaluated against a gold standard fourth generation ELISA (Dia-Pro). Hundred and seventy two serum samples were collected from Lulubriggs Health Center, University of Port Harcourt, Rivers State. All samples were tested for HIV-1/2 using the three rapid test (parallel algorithm and serial algorithm), and retested using the 4th generation ELISA. The results obtained were compared with those from the ELISA. The DetermineTM rapid strips had sensitivity of 66.7%, specificity of 100%, positive predictive value 100%, negative predictive value 89.4% and total agreement of 91.3%. Stat-pak and Core HIV-1/2 rapid strips both had sensitivity of 71.7%, specificity of 100%, positive predictive value 100%, and negative predictive value 90.7% and total agreement of 92.4%. Determine was mostly used by the participant laboratories 18 of 30 (60%). The performance evaluation of the testing algorithm showed that parallel algorithm is more accurate in comparison to serial algorithm. The result of this study reveals that most rapid test are less sensitive and the accuracy of serial testing algorithm is low, this implies that most acute HIV infection will be missed with rapid assay and false negative results will be reported as actual negative with serial algorithm.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70455799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-08-10DOI: 10.4172/2161-0517.1000145
Jamaluddin Majid
African swine fever (ASF) is a viral haemorrhagic fever of pigs with devastating impact on pig production and household income in Africa. Lack of a vaccine and treatment for ASF has increased the dependence on accurate diagnosis as basis for control and possible eradication of the disease. The aim of this study therefore, was to develop and evaluate an in-house sandwich enzyme linked immune sorbent assay (ELISA) using antibodies raised against African swine fever virus (ASFv) isolates from Uganda for diagnosis of ASF. The ASFv was grown in pig alveolar macrophages, the infected cells harvested, lysed and the virus precipitated using polyethylene glycol 6000. The virus was purified on Sepadex G-200 column equilibrated with 50mM Tris-HCl PH 7.2 containing 0.15M NaCl and viral proteins separated by SDS-PAGE. The target protein (vp73), was quantified and used to immunize rabbits to produce polyclonal antibodies against it. The purified immunoglobulin IgG (rabbit anti ASF-vp73) was used for antigen capture in sandwich ELISA. Eighty eight (88) known positive and 176 known negative pig serum samples were used to evaluate assay performance. The diagnostic sensitivity of the ELISA was 82.95% (95% CI, 78-100%), diagnostic specificity was 96.59% (95% CI, 90–100%). Positive and negative predictive values were 92.4% and 91%, respectively. The inter samples coefficient of variation of raw optical density values for known positive samples in different runs was <10% (range 1.1-7.8), while intra sample coefficient of variation ranged from 0.6-5.5% between runs. The developed antigen capture ELISA has a high diagnostic sensitivity and specificity and is therefore good for detection of active ASF infection. However, the developed assay should be further validated using larger sample size under different laboratory conditions and referenced serum samples from different ASF endemic countries.
{"title":"Development and Evaluation of an Antigen Capture Enzyme-LinkedImmunosorbent Assay (AC-ELISA) for the Diagnosis of African Swine fever","authors":"Jamaluddin Majid","doi":"10.4172/2161-0517.1000145","DOIUrl":"https://doi.org/10.4172/2161-0517.1000145","url":null,"abstract":"African swine fever (ASF) is a viral haemorrhagic fever of pigs with devastating impact on pig production and household income in Africa. Lack of a vaccine and treatment for ASF has increased the dependence on accurate diagnosis as basis for control and possible eradication of the disease. The aim of this study therefore, was to develop and evaluate an in-house sandwich enzyme linked immune sorbent assay (ELISA) using antibodies raised against African swine fever virus (ASFv) isolates from Uganda for diagnosis of ASF. The ASFv was grown in pig alveolar macrophages, the infected cells harvested, lysed and the virus precipitated using polyethylene glycol 6000. The virus was purified on Sepadex G-200 column equilibrated with 50mM Tris-HCl PH 7.2 containing 0.15M NaCl and viral proteins separated by SDS-PAGE. The target protein (vp73), was quantified and used to immunize rabbits to produce polyclonal antibodies against it. The purified immunoglobulin IgG (rabbit anti ASF-vp73) was used for antigen capture in sandwich ELISA. Eighty eight (88) known positive and 176 known negative pig serum samples were used to evaluate assay performance. The diagnostic sensitivity of the ELISA was 82.95% (95% CI, 78-100%), diagnostic specificity was 96.59% (95% CI, 90–100%). Positive and negative predictive values were 92.4% and 91%, respectively. The inter samples coefficient of variation of raw optical density values for known positive samples in different runs was <10% (range 1.1-7.8), while intra sample coefficient of variation ranged from 0.6-5.5% between runs. The developed antigen capture ELISA has a high diagnostic sensitivity and specificity and is therefore good for detection of active ASF infection. However, the developed assay should be further validated using larger sample size under different laboratory conditions and referenced serum samples from different ASF endemic countries.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2015-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-0517.1000145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70455718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-07-24DOI: 10.4172/2161-0517.S1.004
Scott Umlauf
{"title":"influenza vaccine produced in E. coli elicits protective HAI titers in healthy adults","authors":"Scott Umlauf","doi":"10.4172/2161-0517.S1.004","DOIUrl":"https://doi.org/10.4172/2161-0517.S1.004","url":null,"abstract":"","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"04 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70461555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-07-24DOI: 10.4172/2161-0517.S1.005
Bipin Thomas
A of vaccines by novel needle-free technology such as jet injection offers an important alternative to needle and syringe and may enhance compliance. In August 2014, needle-free vaccination against influenza was approved with Afluria using PharmaJet® Stratis® jet injection technology. The objective of this post-marketing study was to assess acceptance of and satisfaction with flu vaccination using Afluria delivered in the grocery pharmacy setting via the novel needle-free system. A total of 98 grocery store customers, ages 18-64 were administered needle-free Afluria vaccination during the 20142015 flu season using the PharmaJet device and agreed to complete a short post-administration survey. The population of was 54% female and 74% of all respondents reported receiving a flu shot the prior season. Overall, 89% of subjects reported being satisfied, very satisfied or extremely satisfied with the needle-free flu shot; 83% of subjects reported that they were likely, very likely or definitely going to choose flu vaccination by jet injection next year. The high degree of satisfaction with Afluria delivered through needle-free PharmaJet Stratis suggests that needle-free vaccination with jet injection may be widely accepted, improving compliance in the general adult population which currently has very low rates of immunization against flu.
{"title":"The role of mHealth in the fight against flu","authors":"Bipin Thomas","doi":"10.4172/2161-0517.S1.005","DOIUrl":"https://doi.org/10.4172/2161-0517.S1.005","url":null,"abstract":"A of vaccines by novel needle-free technology such as jet injection offers an important alternative to needle and syringe and may enhance compliance. In August 2014, needle-free vaccination against influenza was approved with Afluria using PharmaJet® Stratis® jet injection technology. The objective of this post-marketing study was to assess acceptance of and satisfaction with flu vaccination using Afluria delivered in the grocery pharmacy setting via the novel needle-free system. A total of 98 grocery store customers, ages 18-64 were administered needle-free Afluria vaccination during the 20142015 flu season using the PharmaJet device and agreed to complete a short post-administration survey. The population of was 54% female and 74% of all respondents reported receiving a flu shot the prior season. Overall, 89% of subjects reported being satisfied, very satisfied or extremely satisfied with the needle-free flu shot; 83% of subjects reported that they were likely, very likely or definitely going to choose flu vaccination by jet injection next year. The high degree of satisfaction with Afluria delivered through needle-free PharmaJet Stratis suggests that needle-free vaccination with jet injection may be widely accepted, improving compliance in the general adult population which currently has very low rates of immunization against flu.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70461226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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