To intelligently remove the yellow-rotten leaf of hydroponic lettuce, a new leaf removal method was proposed. The lettuce position was adjusted for yellow-rotten leaf removal according to the visual recognition and localisation, and then the adsorbed yellow-rotten leaf was lifted by air pipes. Finally, the leaf was clamped and removed along the flipping-tearing-twisting trajectory. The adsorbing pressure, adsorbing position, and clamping position for yellow-rotten leaf removal were confirmed by the adsorbing and stretching tests. To improve the leaf removal success rate, the tearing angle, flipping angle, and torsional time radio were optimised by Box-Behnken tests. A quadratic model for the three factors and leaf removal success rate was established to analyse the orders of significance, and the order of significance for single factor was (i) the tearing angle, (ii) the flipping angle, and (iii) the torsional time radio. The order of significance for interaction terms was (i) the flipping angle and tearing angle, and (ii) the flipping angle and torsional time ratio. The solved optimal combination of factors was a flipping angle of 100.5°, a tearing angle of 131.0°, and a torsional time ratio of 0.68, which gave the maximum leaf removal success rate. The optimal combination of factors was verified, and the leaf removal process was shot by high speed camera. The verification tests showed that the maximum leaf removal success rate was 82.8%, and the leaf removal process took 6.58 s, meeting the requirements of yellow-rotten leaf removal for hydroponic lettuce.