The aim of this review was to summarise the information about the cases of highly pathogenic avian influenza in Bulgaria during the last two decades. According to the EMPRES-I FAO data, from January 2006 to the end of 2019, 141 HPAI outbreaks have been registered in Bulgaria, with two identified virus serotypes (H5N1 and H5N8). The H5N1 outbreaks were reported in 2006, 2010 and 2015. Almost all H5N1 cases were observed in wild birds, with only one outbreak in domestic chickens in a backyard farm in 2015. From 2016 to the end of 2019, 132 outbreaks of HPAI identified as H5N8 were recorded: sixteen in wild birds and the other 116 - in domestic poultry. Large farms with fattening ducks used for foie gras production in four administrative districts (Plovdiv, Haskovo, Stara Zagora, Dobrich) were mainly affected.
{"title":"Highly pathogenic avian influenza in Bulgaria - a review","authors":"G. Stoimenov","doi":"10.15547/bjvm.2020-0062","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0062","url":null,"abstract":"The aim of this review was to summarise the information about the cases of highly pathogenic avian influenza in Bulgaria during the last two decades. According to the EMPRES-I FAO data, from January 2006 to the end of 2019, 141 HPAI outbreaks have been registered in Bulgaria, with two identified virus serotypes (H5N1 and H5N8). The H5N1 outbreaks were reported in 2006, 2010 and 2015. Almost all H5N1 cases were observed in wild birds, with only one outbreak in domestic chickens in a backyard farm in 2015. From 2016 to the end of 2019, 132 outbreaks of HPAI identified as H5N8 were recorded: sixteen in wild birds and the other 116 - in domestic poultry. Large farms with fattening ducks used for foie gras production in four administrative districts (Plovdiv, Haskovo, Stara Zagora, Dobrich) were mainly affected.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83369787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Rezanejad, S. Ojagh, M. Heidarieh, M. Raeisi, A. Alishahi, G. Rafiee, S. H. Mousavi, S. Nabizadeh
The effect of the gamma-irradiated extract of rosemary and butylated hydroxyanisole (BHA) antioxidant on sensory, chemical and microbiological changes of rainbow trout fillets stored at 4 ± 1°C was investigated for 16 days. The fillets were divided into: control (C), 750 and 1500 rosemary extract (RE) (immersed in 750 and 1500 ppm rosemary extract); 250 and 500 gamma-irradiated rosemary (GIR), (immersed in 250 and 500 ppm gamma-irradiated rosemary) and 250 and 500 BHA (immersed in 250 and 500 ppm BHA). The shelf life of fillets was reported to be 8 days for C, and 12 days for 500 GIR group according sensory, microbiological and chemical parameters. The pH, peroxide value (PV), total volatile basic nitrogen (TVB-N), free fatty acid (FFA), thiobarbituric acid (TBA), psychrotrophic counts (PTC), and total viable aerobic bacterial counts (TVC) values were 7.62 ± 0.21, 17.17 ± 1.6, 51.07 ± 1.1, 6.05 ± 0.13, 3.42 ± 0.18, 12.03 ± 0.19, and 13.16 ± 0.16 respectively for C group and also, 7.42 ± 0.16, 15.2 ± 1.7, 42.5 ± 1.5, 4.48 ± 0.39, 2.59 ± 0.15, 9.98 ± 0.15, and 10.5 ± 0.15 respectively for 500 GIR group. Finally, the following trend in effectiveness was reported: gamma-irradiated rosemary PBS extract > BHA > rosemary PBS extract.
{"title":"Gamma-irradiated rosemary (Rosmarinus officinalis) dips treatment effect on quality of rainbow trout (Oncorhynchus mykiss) fillets during refrigerated storage","authors":"R. Rezanejad, S. Ojagh, M. Heidarieh, M. Raeisi, A. Alishahi, G. Rafiee, S. H. Mousavi, S. Nabizadeh","doi":"10.15547/bjvm.2020-0038","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0038","url":null,"abstract":"The effect of the gamma-irradiated extract of rosemary and butylated hydroxyanisole (BHA) antioxidant on sensory, chemical and microbiological changes of rainbow trout fillets stored at 4 ± 1°C was investigated for 16 days. The fillets were divided into: control (C), 750 and 1500 rosemary extract (RE) (immersed in 750 and 1500 ppm rosemary extract); 250 and 500 gamma-irradiated rosemary (GIR), (immersed in 250 and 500 ppm gamma-irradiated rosemary) and 250 and 500 BHA (immersed in 250 and 500 ppm BHA). The shelf life of fillets was reported to be 8 days for C, and 12 days for 500 GIR group according sensory, microbiological and chemical parameters. The pH, peroxide value (PV), total volatile basic nitrogen (TVB-N), free fatty acid (FFA), thiobarbituric acid (TBA), psychrotrophic counts (PTC), and total viable aerobic bacterial counts (TVC) values were 7.62 ± 0.21, 17.17 ± 1.6, 51.07 ± 1.1, 6.05 ± 0.13, 3.42 ± 0.18, 12.03 ± 0.19, and 13.16 ± 0.16 respectively for C group and also, 7.42 ± 0.16, 15.2 ± 1.7, 42.5 ± 1.5, 4.48 ± 0.39, 2.59 ± 0.15, 9.98 ± 0.15, and 10.5 ± 0.15 respectively for 500 GIR group. Finally, the following trend in effectiveness was reported: gamma-irradiated rosemary PBS extract > BHA > rosemary PBS extract.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77725023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Jalilzadeh-Amin, Y. Nozohour, M. Maham, F. Malekifard
Clinical trypanosomiasis in sheep is described for the first time in Iran after history taking, complete physical examination and blood samples collected for laboratory investigations. The client emphasised on animal weight loss dating back to one week ago. The clinical examination revealed enlargements of the pre-scapular and pre-femoral lymph nodes and pale mucous membranes. Laboratory investigation showed that the sheep had anaemia with hyperproteinaemia. Thin blood smear examination revealed the presence of Trypanosoma. Treatment included diminazene aceturate (3.5 mg/kg, IM) for three consecutive days, flunixin meglumine (1.1 mg/kg, IM), and oxytetracycline (10 mg/kg, IM). The examination of the blood film after treatment showed no parasite. Trypanosomiasis may occur in our climatic condition in sheep. In two cases studied here, two consecutive doses of diminazene aceturate administration led to clinical cure. The control of trypanosomiasis in farms may be possible through methods for the control of vectors, such as prophylactic treatment and observing of hygiene in husbandry systems for animals at risk
{"title":"First report of surra (Trypanosoma evansi infection) in Iranian sheep","authors":"G. Jalilzadeh-Amin, Y. Nozohour, M. Maham, F. Malekifard","doi":"10.15547/bjvm.2020-0117","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0117","url":null,"abstract":"Clinical trypanosomiasis in sheep is described for the first time in Iran after history taking, complete physical examination and blood samples collected for laboratory investigations. The client emphasised on animal weight loss dating back to one week ago. The clinical examination revealed enlargements of the pre-scapular and pre-femoral lymph nodes and pale mucous membranes. Laboratory investigation showed that the sheep had anaemia with hyperproteinaemia. Thin blood smear examination revealed the presence of Trypanosoma. Treatment included diminazene aceturate (3.5 mg/kg, IM) for three consecutive days, flunixin meglumine (1.1 mg/kg, IM), and oxytetracycline (10 mg/kg, IM). The examination of the blood film after treatment showed no parasite. Trypanosomiasis may occur in our climatic condition in sheep. In two cases studied here, two consecutive doses of diminazene aceturate administration led to clinical cure. The control of trypanosomiasis in farms may be possible through methods for the control of vectors, such as prophylactic treatment and observing of hygiene in husbandry systems for animals at risk","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78839620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Behavioural disorders, including feather pecking and cannibalism, are a common problem in both domestic and wild birds. The consequences of this behaviour on welfare of birds incur serious economic losses. Pecking behaviour in birds is either normal or injurious. The type of normal pecking behaviour includes non-aggressive feather pecking – allopreening and autopreening. Aggressive feather pecking aimed at maintenance and establishment of hierarchy in the flock is not associated to feathering damage. Injurious pecking causes damage of individual feathers and of feathering as a whole. Two clinical presentations of feather pecking are known in birds. The gentle feather pecking causes minimum damage; it is further divided into normal and stereotyped with bouts; it could however evolve into severe feather pecking manifested with severe pecking, pulling and removal, even consumption of feathers of the victim, which experiences pain. Severe feather pecking results in bleeding from feather follicle, deterioration of plumage and appearance of denuded areas on victim’s body. Prolonged feather pecking leads to tissue damage and consequently, cannibalism. The numerous clinical presentations of the latter include pecking of the back, abdomen, neck and wings. Vent pecking and abdominal pecking incur important losses especially during egg-laying. In young birds, pulling and pecking of toes of legs is encountered. All forms of cannibalistic pecking increase mortality rates in birds. Transition of various pecking types from one into another could be seen, while the difference between gentle, severe feather pecking and cannibalism is not always distinct.
{"title":"Types and clinical presentation of damaging behaviour - feather pecking and cannibalism in birds","authors":"S. Nikolov, D. Kanakov","doi":"10.15547/bjvm.2020-0027","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0027","url":null,"abstract":"Behavioural disorders, including feather pecking and cannibalism, are a common problem in both domestic and wild birds. The consequences of this behaviour on welfare of birds incur serious economic losses. Pecking behaviour in birds is either normal or injurious. The type of normal pecking behaviour includes non-aggressive feather pecking – allopreening and autopreening. Aggressive feather pecking aimed at maintenance and establishment of hierarchy in the flock is not associated to feathering damage. Injurious pecking causes damage of individual feathers and of feathering as a whole. Two clinical presentations of feather pecking are known in birds. The gentle feather pecking causes minimum damage; it is further divided into normal and stereotyped with bouts; it could however evolve into severe feather pecking manifested with severe pecking, pulling and removal, even consumption of feathers of the victim, which experiences pain. Severe feather pecking results in bleeding from feather follicle, deterioration of plumage and appearance of denuded areas on victim’s body. Prolonged feather pecking leads to tissue damage and consequently, cannibalism. The numerous clinical presentations of the latter include pecking of the back, abdomen, neck and wings. Vent pecking and abdominal pecking incur important losses especially during egg-laying. In young birds, pulling and pecking of toes of legs is encountered. All forms of cannibalistic pecking increase mortality rates in birds. Transition of various pecking types from one into another could be seen, while the difference between gentle, severe feather pecking and cannibalism is not always distinct.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87798810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infectious bursal disease (IBD) virus is considered one of the commonest immunosuppressive diseases in chickens. The aim of this study was to investigate the concurrent occurrence of subclinical IBD in respiratory complex infections caused by avian influenza (AI, H9N2) virus and infectious bronchitis (IB) virus in broilers. During this study, 800 tissue samples of the trachea, caecal tonsil, spleen, and bursa of Fabricius and 400 blood samples were collected from 20 respiratory complex infected flocks. Detection of pathogens in the tissue samples was performed by RT-PCR for amplification of the VP2 gene of IBD, HA region of AI, and S1 gene of IB viruses. The amplified products were subjected to nucleotide sequence analysis. Blood samples were also tested for the detection of antibodies against IBV by using ELISA and against AIV via using the HI test. Molecular results showed that the tissue samples were positive for field isolates of subclinical IBD (45%), IB (45%), and AI-H9N2 (25%). Co-infections of IBD and IB (30%), IBD and AI (20%), and IBD, IB, and AI (5%) were also detected. Serological results indicated that subclinical IBD infected flocks had lower (P<0.05) antibody titres against IB and AI. In conclusion, prior exposure of broilers to IBD virus increased the incidence of respiratory complex caused by IBV and AIV in broilers, and vaccination against IBD is inevitable to reduce subclinical IBD to minimise the incidence/severity of respiratory complex diseases via improving immune responses to commonly used vaccines in broilers.
{"title":"Concurrent occurrence of infectious bursal disease and respiratory complex caused by infectious bronchitis and avian influenza (H9N2) in broilers","authors":"J. Taifebagherlu, A. Talebi, M. Allymehr","doi":"10.15547/bjvm.2020-0142","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0142","url":null,"abstract":"Infectious bursal disease (IBD) virus is considered one of the commonest immunosuppressive diseases in chickens. The aim of this study was to investigate the concurrent occurrence of subclinical IBD in respiratory complex infections caused by avian influenza (AI, H9N2) virus and infectious bronchitis (IB) virus in broilers. During this study, 800 tissue samples of the trachea, caecal tonsil, spleen, and bursa of Fabricius and 400 blood samples were collected from 20 respiratory complex infected flocks. Detection of pathogens in the tissue samples was performed by RT-PCR for amplification of the VP2 gene of IBD, HA region of AI, and S1 gene of IB viruses. The amplified products were subjected to nucleotide sequence analysis. Blood samples were also tested for the detection of antibodies against IBV by using ELISA and against AIV via using the HI test. Molecular results showed that the tissue samples were positive for field isolates of subclinical IBD (45%), IB (45%), and AI-H9N2 (25%). Co-infections of IBD and IB (30%), IBD and AI (20%), and IBD, IB, and AI (5%) were also detected. Serological results indicated that subclinical IBD infected flocks had lower (P<0.05) antibody titres against IB and AI. In conclusion, prior exposure of broilers to IBD virus increased the incidence of respiratory complex caused by IBV and AIV in broilers, and vaccination against IBD is inevitable to reduce subclinical IBD to minimise the incidence/severity of respiratory complex diseases via improving immune responses to commonly used vaccines in broilers.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87926218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Dobreva, M. Koprinarova, A. Bratchkova, S. Danova
Several Lactobacillus species are accepted as microorganisms with Qualified Presumption of Safety (QPS) in the EFSA’s list. One of them, Lactobacillus plantarum is a widely distributed species with a proven probiotic potential and technological relevance. In addition, every strain must complete several requirements, before implementation. Antibiotic susceptibility is one of EFSA’s important criteria regarding the safety of probiotics. The reason is to avoid any possibility of antibiotic resistance genes transfer to opportunistic pathogens in the gut. In the present study 14 Lactobacillus plantarum strains were assessed for susceptibility to 21 antibiotics from different groups. A high number of resistant strains was determined toward 12 antibiotics (penicillins – penicillin, piperacillin; IIIth generation cephalosporins – cefotaxime, ceftriaxone, ceftazidime; glycopeptides – vancomycin; tetracyclines – tetracycline; aminoglycosides – gentamicin; macrolides – clarithromycin; quinolones – nalidixic acid, ciprofloxacin, levofloxacin). Concerning the other tested antibiotics, strain-specific antibiotic-sensitivity patterns were observed. Antibiotic resistance was also discussed as an advantage in the selection of probiotic strains, however only when it is not transferable. Estimated susceptibility patterns of some of tested candidate probiotic strains are also important, considering the use of the latter as agents accompanying antibiotic therapy
{"title":"Antibiotic susceptibility of Lactobacillus plantarum strains, isolated from katak","authors":"L. Dobreva, M. Koprinarova, A. Bratchkova, S. Danova","doi":"10.15547/bjvm.2020-0072","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0072","url":null,"abstract":"Several Lactobacillus species are accepted as microorganisms with Qualified Presumption of Safety (QPS) in the EFSA’s list. One of them, Lactobacillus plantarum is a widely distributed species with a proven probiotic potential and technological relevance. In addition, every strain must complete several requirements, before implementation. Antibiotic susceptibility is one of EFSA’s important criteria regarding the safety of probiotics. The reason is to avoid any possibility of antibiotic resistance genes transfer to opportunistic pathogens in the gut. In the present study 14 Lactobacillus plantarum strains were assessed for susceptibility to 21 antibiotics from different groups. A high number of resistant strains was determined toward 12 antibiotics (penicillins – penicillin, piperacillin; IIIth generation cephalosporins – cefotaxime, ceftriaxone, ceftazidime; glycopeptides – vancomycin; tetracyclines – tetracycline; aminoglycosides – gentamicin; macrolides – clarithromycin; quinolones – nalidixic acid, ciprofloxacin, levofloxacin). Concerning the other tested antibiotics, strain-specific antibiotic-sensitivity patterns were observed. Antibiotic resistance was also discussed as an advantage in the selection of probiotic strains, however only when it is not transferable. Estimated susceptibility patterns of some of tested candidate probiotic strains are also important, considering the use of the latter as agents accompanying antibiotic therapy","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88251519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Sebbar, K. Zro, K. Id Sidi Yahia, M. Elouennass, A. Filali-Maltouf, B. Belkadi
The present study is the first report aimed to determine the antibiotic susceptibility profiles of Mannheimia haemolytica and Pasteurella multocida serogroup A Moroccan isolates. Each isolate was tested for sensitivity to amoxicillin (A), amoxicillin/clavulanic acid (AMC), gentamicin (CN), streptomycin (STR), florfenicol (FFC), doxycycline (DO), erythromycin (E), spiramycin (SP), nalidixic acid (NA), flumequine (UB), enrofloxacin (ENF) and sulfamethoxazole (SXT). All isolates showed resistance to the antibiotics tested at a rate greater than 14%, except for one P. multocida isolate which had no resistance profile against AMC. The highest level of resistance was found against NA for P. multocida (100%) and against UB (82.4%) for M. haemolytica. The sensitivity rates for P. multocida were between 0 (against NA) and 85.7% (against STR, AMC, FFC), whereas sensitivity of M. haemolytica isolates ranged from 17.6% against UB and 79.4% against AMC. For both Pasteurellaceae species, the AMC was the most effective antimicrobial agent, however multi-drug resistance was observed in all isolates raising the interest to monitor the antimicrobial susceptibility of Pasteurellaceae species to determine appropriate antibiotic for treatment of pasteurellosis.
{"title":"Antimicrobial susceptibility screening test of Mannheimia haemolytica and Pasteurella multocida (serogroup A) Moroccan strains isolated from ruminants","authors":"G. Sebbar, K. Zro, K. Id Sidi Yahia, M. Elouennass, A. Filali-Maltouf, B. Belkadi","doi":"10.15547/bjvm.2019-0109","DOIUrl":"https://doi.org/10.15547/bjvm.2019-0109","url":null,"abstract":"The present study is the first report aimed to determine the antibiotic susceptibility profiles of Mannheimia haemolytica and Pasteurella multocida serogroup A Moroccan isolates. Each isolate was tested for sensitivity to amoxicillin (A), amoxicillin/clavulanic acid (AMC), gentamicin (CN), streptomycin (STR), florfenicol (FFC), doxycycline (DO), erythromycin (E), spiramycin (SP), nalidixic acid (NA), flumequine (UB), enrofloxacin (ENF) and sulfamethoxazole (SXT). All isolates showed resistance to the antibiotics tested at a rate greater than 14%, except for one P. multocida isolate which had no resistance profile against AMC. The highest level of resistance was found against NA for P. multocida (100%) and against UB (82.4%) for M. haemolytica. The sensitivity rates for P. multocida were between 0 (against NA) and 85.7% (against STR, AMC, FFC), whereas sensitivity of M. haemolytica isolates ranged from 17.6% against UB and 79.4% against AMC. For both Pasteurellaceae species, the AMC was the most effective antimicrobial agent, however multi-drug resistance was observed in all isolates raising the interest to monitor the antimicrobial susceptibility of Pasteurellaceae species to determine appropriate antibiotic for treatment of pasteurellosis.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88752286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Chalmeh, M. Pourjafar, K. Badiei, M. MAZROUEI SEBDANI, N. Hatami Nasar, S. M. M. Heidari
The characteristics associated with equine peripartum period are different from those of other periods. Information concerning the values and dynamic changes of circulating peripartum endocrine, metabolic, and haematologic indices may improve the understanding of the whole body haemostatic patterns which lead to parturition, lactogenesis, and estrus in the horse. The present study aimed to evaluate these changes around parturition in Darehshori breed. For this purpose, blood sampling was performed on six clinically healthy Darehshori mares (10 years old) about one week prior to parturition, three days following parturition, at the beginning of estrus, and one week after estrus. Circulating values of glucose, estrogen, progesterone, cortisol, T3, T4, TG, cholesterol, HDL, LDL, VLDL, RBC, HGB, HCT, MCV, MCH, MCHC, WBC, neutrophil, lymphocyte, and PLT were assessed in all samples. Progesterone concentrations were minimum before parturition and increased significantly one week after foaling heat (P=0.035). Cortisol level was significantly higher during foaling heat compared with other periods (P=0.029). T4 levels significantly increased from pre- to post-partum periods (P=0.001), but T3 showed increased and decreased values during the study period. All serum lipid profile parameters were significantly reduced from pre to postpartum period (P<0.05). Haematological parameters in mares significantly changed during the peripartum period (P<0.05). Based on the results, the metabolic, haematological, endocrinological, and stress indices in Darehshori mares were found to change around parturition and the first estrus. These changes were largely dependent on hormonal mechanisms related to pregnancy and parturition occurring in normal mares.
{"title":"Circulating endocrine, metabolic, and haematologic indices during the peripartum period of Darehshori mares","authors":"A. Chalmeh, M. Pourjafar, K. Badiei, M. MAZROUEI SEBDANI, N. Hatami Nasar, S. M. M. Heidari","doi":"10.15547/bjvm.2020-0052","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0052","url":null,"abstract":"The characteristics associated with equine peripartum period are different from those of other periods. Information concerning the values and dynamic changes of circulating peripartum endocrine, metabolic, and haematologic indices may improve the understanding of the whole body haemostatic patterns which lead to parturition, lactogenesis, and estrus in the horse. The present study aimed to evaluate these changes around parturition in Darehshori breed. For this purpose, blood sampling was performed on six clinically healthy Darehshori mares (10 years old) about one week prior to parturition, three days following parturition, at the beginning of estrus, and one week after estrus. Circulating values of glucose, estrogen, progesterone, cortisol, T3, T4, TG, cholesterol, HDL, LDL, VLDL, RBC, HGB, HCT, MCV, MCH, MCHC, WBC, neutrophil, lymphocyte, and PLT were assessed in all samples. Progesterone concentrations were minimum before parturition and increased significantly one week after foaling heat (P=0.035). Cortisol level was significantly higher during foaling heat compared with other periods (P=0.029). T4 levels significantly increased from pre- to post-partum periods (P=0.001), but T3 showed increased and decreased values during the study period. All serum lipid profile parameters were significantly reduced from pre to postpartum period (P<0.05). Haematological parameters in mares significantly changed during the peripartum period (P<0.05). Based on the results, the metabolic, haematological, endocrinological, and stress indices in Darehshori mares were found to change around parturition and the first estrus. These changes were largely dependent on hormonal mechanisms related to pregnancy and parturition occurring in normal mares.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81348001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Mousavinasab, M. Bozorgmehrifard, S. M. Kiaei, S. Rahbari, S. Charkhkar
Coccidiosis is the most important intestinal parasitic disease of broiler chickens in poultry industry. Because of the increasing resistance to anticoccidial agents and presence of their residues in meat and eggs, it is necessary to find safe and new anticoccidial compounds. This study was conducted to compare the effects of two herbal compounds, including Artemisia sieberi and Curcuma longa, and their mix with a chemical anticoccidial drug on broilers’ performance during a mixed coccidian challenge. A total of 216, one-day-old Ross 308 broilers were randomly divided into six groups. Different herbal extracts and one chemical anticoccidial agent were used in each group. Five groups were infected with a mixture of Eimeria sporulated oocysts at the age of 21 days with crop gavage. Body weight and feed intake were measured then feed conversion ratio was calculated on a weekly basis. Mortality was recorded when occurred throughout the experimental period. Oocysts excretions and lesion scores were investigated weekly up to three weeks after infection. Eimeria-challenged birds had a reduction in growth parameters compared to the uninfected birds (P<0.001); the best performance values were recorded for the groups treated with a mix of two herbal extracts and amprolium ethopabate (P<0.05). The groups treated with herbal extracts had a significantly reduced oocyst excretion per gram of faeces compared to the positive control group. Lesion score of the amprolium ethopabate group was better than those of the other groups. As a conclusion, herbal extracts, especially a mix of them, could be effective in controlling coccidiosis and its complications.
{"title":"Comparison of the effects of herbal compounds and chemical drugs for control of coccidiosis in broiler chickens","authors":"S. Mousavinasab, M. Bozorgmehrifard, S. M. Kiaei, S. Rahbari, S. Charkhkar","doi":"10.15547/bjvm.2020-0005","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0005","url":null,"abstract":"Coccidiosis is the most important intestinal parasitic disease of broiler chickens in poultry industry. Because of the increasing resistance to anticoccidial agents and presence of their residues in meat and eggs, it is necessary to find safe and new anticoccidial compounds. This study was conducted to compare the effects of two herbal compounds, including Artemisia sieberi and Curcuma longa, and their mix with a chemical anticoccidial drug on broilers’ performance during a mixed coccidian challenge. A total of 216, one-day-old Ross 308 broilers were randomly divided into six groups. Different herbal extracts and one chemical anticoccidial agent were used in each group. Five groups were infected with a mixture of Eimeria sporulated oocysts at the age of 21 days with crop gavage. Body weight and feed intake were measured then feed conversion ratio was calculated on a weekly basis. Mortality was recorded when occurred throughout the experimental period. Oocysts excretions and lesion scores were investigated weekly up to three weeks after infection. Eimeria-challenged birds had a reduction in growth parameters compared to the uninfected birds (P<0.001); the best performance values were recorded for the groups treated with a mix of two herbal extracts and amprolium ethopabate (P<0.05). The groups treated with herbal extracts had a significantly reduced oocyst excretion per gram of faeces compared to the positive control group. Lesion score of the amprolium ethopabate group was better than those of the other groups. As a conclusion, herbal extracts, especially a mix of them, could be effective in controlling coccidiosis and its complications.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90020575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Haji Mohammad, H. Koohsari, S. H. Hosseini Ghaboos
Royal jelly is one of the most important bee products. The biological activities of royal jelly can be influenced by various factors such as geographic origin, climatic conditions, vegetation. This study was carried out to evaluate the antibacterial and antioxidant activity of royal jelly samples collected from beehives from different geographical regions including mountain, coastal and plain regions in northern Iran. Antibacterial activity of royal jelly samples against ten bacteria was determined using agar well diffusion method. The MIC and MBC of royal jelly samples were determined by the broth microdilution method. Folin-Ciocâlteu reagent and reaction with DPPH were used to determine the total phenolic content and antioxidant potential of royal jelly samples, respectively. The MIC of samples ranged from 0.78 to 12.5% and MBC - from 3.12 to 50%. Samples collected from mountain regions showed the highest antibacterial activity with MIC for Gram-positive bacteria from 0.78 to 1.56% and for Gram-negative bacteria: from 1.56 to 3.12%. The total phenolic content and DPPH radical scavenging activity in royal jelly samples of the mountain region was significantly higher than those from the two regions with other climates. The results of this study indicated that the climate of the geographic region of sampling location had an effect on the antibacterial and antioxidant activity of royal jelly which may be due to differences in plant vegetation and the origin of the flowers of bees.
{"title":"Antibacterial and antioxidant activity of royal jelly collected from geographical regions with different climates in the north of Iran","authors":"F. Haji Mohammad, H. Koohsari, S. H. Hosseini Ghaboos","doi":"10.15547/bjvm.2020-0133","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0133","url":null,"abstract":"Royal jelly is one of the most important bee products. The biological activities of royal jelly can be influenced by various factors such as geographic origin, climatic conditions, vegetation. This study was carried out to evaluate the antibacterial and antioxidant activity of royal jelly samples collected from beehives from different geographical regions including mountain, coastal and plain regions in northern Iran. Antibacterial activity of royal jelly samples against ten bacteria was determined using agar well diffusion method. The MIC and MBC of royal jelly samples were determined by the broth microdilution method. Folin-Ciocâlteu reagent and reaction with DPPH were used to determine the total phenolic content and antioxidant potential of royal jelly samples, respectively. The MIC of samples ranged from 0.78 to 12.5% and MBC - from 3.12 to 50%. Samples collected from mountain regions showed the highest antibacterial activity with MIC for Gram-positive bacteria from 0.78 to 1.56% and for Gram-negative bacteria: from 1.56 to 3.12%. The total phenolic content and DPPH radical scavenging activity in royal jelly samples of the mountain region was significantly higher than those from the two regions with other climates. The results of this study indicated that the climate of the geographic region of sampling location had an effect on the antibacterial and antioxidant activity of royal jelly which may be due to differences in plant vegetation and the origin of the flowers of bees.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80307707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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