L. Dobreva, S. Danova, V. Georgieva, S. Georgieva, M. Koprinarova
Lactic acid bacteria (LAB) may contribute to the food safety. In the present study, the antagonistic activity of 45 Bulgarian Lactobacillus homo- and heterofermentative strains of human and dairy origin, and 4 multibacterial formulas against Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) was assessed. In vitro tests were performed in different model systems - milk, soymilk, De Man Rogose Sharp (MRS) medium, to simulate real conditions in the food chain. The highest antagonistic activity was observed with cell-free supernatants of exponential MRS broth cultures of the strains isolated from breast milk, followed by lactobacilli from white brined and green cheese. The detected antimicrobial activity against the pathogen was strain-specific and depended on the culture conditions. Lactobacillus (reclassified as Lactoplantibacillus) plantarum strains, cultivated in skimmed milk and whey protein medium, were able to inhibit S. Typhimurium growth, while a limited inhibitory activity was detected for fermented soymilks. A bacteriocinogenic Ligilactobacillus (the previous Lactobacillus) salivarius strain reduced the number of living pathogenic cells during co-cultivation in whole milk. The inhibition was significant only when L. salivarius was inoculated in predominance. In case of underrepresented LAB number, S. Typhimurium over-growth was observed. Eight lactobacilli in combination as a multibacterial co-culture expressed synergic antagonistic effect against Salmonella and were pre-selected as promising. Further characterisation of their active metabolites, however, is needed before their classification as bio-protective agents.
{"title":"Anti-Salmonella activity of lactobacilli from different habitats","authors":"L. Dobreva, S. Danova, V. Georgieva, S. Georgieva, M. Koprinarova","doi":"10.15547/bjvm.2395","DOIUrl":"https://doi.org/10.15547/bjvm.2395","url":null,"abstract":"Lactic acid bacteria (LAB) may contribute to the food safety. In the present study, the antagonistic activity of 45 Bulgarian Lactobacillus homo- and heterofermentative strains of human and dairy origin, and 4 multibacterial formulas against Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) was assessed. In vitro tests were performed in different model systems - milk, soymilk, De Man Rogose Sharp (MRS) medium, to simulate real conditions in the food chain. The highest antagonistic activity was observed with cell-free supernatants of exponential MRS broth cultures of the strains isolated from breast milk, followed by lactobacilli from white brined and green cheese. The detected antimicrobial activity against the pathogen was strain-specific and depended on the culture conditions. Lactobacillus (reclassified as Lactoplantibacillus) plantarum strains, cultivated in skimmed milk and whey protein medium, were able to inhibit S. Typhimurium growth, while a limited inhibitory activity was detected for fermented soymilks. A bacteriocinogenic Ligilactobacillus (the previous Lactobacillus) salivarius strain reduced the number of living pathogenic cells during co-cultivation in whole milk. The inhibition was significant only when L. salivarius was inoculated in predominance. In case of underrepresented LAB number, S. Typhimurium over-growth was observed. Eight lactobacilli in combination as a multibacterial co-culture expressed synergic antagonistic effect against Salmonella and were pre-selected as promising. Further characterisation of their active metabolites, however, is needed before their classification as bio-protective agents.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84676609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The freeze-thaw process results in structural and functional damages caused by overaccumulation of reactive oxygen species (ROS). Addition of antioxidants to semen extenders is of a great importance to overcome this oxidative damage. The study objective was to evaluate the consequence of using Tris-citric acid fructose egg yolk (TCFY) extender supplemented with a combination of turmeric extract and dimethyl sulfoxide (DMSO) [TTD] on sperm freezability and fertility. From five tubes (each containing 5 mL TCFY), the first tube contained neither turmeric extract nor DMSO and was kept as control. The other four tubes contained 1.5% DMSO plus 100 µL/5 mL, 200 µL/5 mL, 300 µL/5 mL and 400 µL/5 mL turmeric extract. Semen samples were pooled and extended to reach an application of 60×106 sperm/mL (TT1D to TT4D, respectively). Diluted semen was exposed to the freezing protocol. The post cooling results revealed significant improvement in percent of alive spermatozoa in TT1D, significant decrease in sperm abnormalities in all concentrations used, significant improvement in intact acrosome percentage in TT1D, TT2D and TT4D. The post thawing results exhibited significant improvement in sperm motility in TT1D, TT2D and TT4D; significant amelioration of sperm membrane integrity (HOST) in TT1D, TT2D, TT3D and TT4D. Acrosome integrity was maintained in all concentrations as in the control. It was concluded that TT1D revealed the best semen quality in cooled semen; and TT1D, TT2D exhibited the superior post thawing semen quality. In addition, conception rate (CR) of the post-thawed semen was ameliorated in TT1D, TT2D, TT3D and TT4D with TT1D being the best one with this regard.
{"title":"Effect of turmeric extract and dimethyl sulfoxide on buffalo semen freezability and fertility","authors":"R. El-Sheshtawy","doi":"10.15547/bjvm.2020-0124","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0124","url":null,"abstract":"The freeze-thaw process results in structural and functional damages caused by overaccumulation of reactive oxygen species (ROS). Addition of antioxidants to semen extenders is of a great importance to overcome this oxidative damage. The study objective was to evaluate the consequence of using Tris-citric acid fructose egg yolk (TCFY) extender supplemented with a combination of turmeric extract and dimethyl sulfoxide (DMSO) [TTD] on sperm freezability and fertility. From five tubes (each containing 5 mL TCFY), the first tube contained neither turmeric extract nor DMSO and was kept as control. The other four tubes contained 1.5% DMSO plus 100 µL/5 mL, 200 µL/5 mL, 300 µL/5 mL and 400 µL/5 mL turmeric extract. Semen samples were pooled and extended to reach an application of 60×106 sperm/mL (TT1D to TT4D, respectively). Diluted semen was exposed to the freezing protocol. The post cooling results revealed significant improvement in percent of alive spermatozoa in TT1D, significant decrease in sperm abnormalities in all concentrations used, significant improvement in intact acrosome percentage in TT1D, TT2D and TT4D. The post thawing results exhibited significant improvement in sperm motility in TT1D, TT2D and TT4D; significant amelioration of sperm membrane integrity (HOST) in TT1D, TT2D, TT3D and TT4D. Acrosome integrity was maintained in all concentrations as in the control. It was concluded that TT1D revealed the best semen quality in cooled semen; and TT1D, TT2D exhibited the superior post thawing semen quality. In addition, conception rate (CR) of the post-thawed semen was ameliorated in TT1D, TT2D, TT3D and TT4D with TT1D being the best one with this regard.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85256993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Ilieva, D. Mihaylova, A. IIlyazova, P. Penchev, D. Abadjieva, E. Kistanova
The herbal protocols, used instead of hormones in buffalo breeding for reproduction optimisation, do not provide information of their effect on the milk quality of treated animals. This research analysed whether the herbal preparation AyuFertin (Indian Herbs Specialities Pvt. Ltd, Saharanpur), applied in postpartum period for recovering the ovary cycling, had a side effect on the milk composition. The experiment was conducted with buffaloes of the Bulgarian Murrah breed at average age of 50 months during May-June 2019, divided into control (n=6) and experimental (n=7) groups. The experimental buffaloes received AyuFertin orally for three consecutive days at a dose of 3 g/100 kg live weight since the 21st postpartum day. The animals without signs of estrus were treated again after 10 days. The milk samples were collected at test-days, before and after treatments. The fatty acid composition of the milk and Ayufertin was evaluated by gas chromatography-mass spectrometry analysis. Quantification of the identified fatty acids in milk was made by the area normalisation method. In general, the fatty acids composition did not differ after the treatment with AyuFertin. Deviation in the amounts of some fatty acids was established. The content of medium-chain fatty acids such as lauric and capric was decreased, while the amount of long-chain acids such as 11-hexadecanoic and stearic was increased in the experimental group. The main active components of AyuFertin - trienoic acids were not transferred into the milk of treated animals. In conclusion, due to the ability of AyuFertin to affect the fatty acids amount in the milk of treated buffaloes, farmers should strictly follow the manufacturer’s recommendation for the dose and duration of AyuFertin treatment to avoid negative effects on milk quality.
{"title":"Effects of the herbal preparation AyuFertin, used for anestrus overcome, on fatty acids composition of milk in Bulgarian Murrah buffaloes","authors":"Y. Ilieva, D. Mihaylova, A. IIlyazova, P. Penchev, D. Abadjieva, E. Kistanova","doi":"10.15547/bjvm.2384","DOIUrl":"https://doi.org/10.15547/bjvm.2384","url":null,"abstract":"The herbal protocols, used instead of hormones in buffalo breeding for reproduction optimisation, do not provide information of their effect on the milk quality of treated animals. This research analysed whether the herbal preparation AyuFertin (Indian Herbs Specialities Pvt. Ltd, Saharanpur), applied in postpartum period for recovering the ovary cycling, had a side effect on the milk composition. The experiment was conducted with buffaloes of the Bulgarian Murrah breed at average age of 50 months during May-June 2019, divided into control (n=6) and experimental (n=7) groups. The experimental buffaloes received AyuFertin orally for three consecutive days at a dose of 3 g/100 kg live weight since the 21st postpartum day. The animals without signs of estrus were treated again after 10 days. The milk samples were collected at test-days, before and after treatments. The fatty acid composition of the milk and Ayufertin was evaluated by gas chromatography-mass spectrometry analysis. Quantification of the identified fatty acids in milk was made by the area normalisation method. In general, the fatty acids composition did not differ after the treatment with AyuFertin. Deviation in the amounts of some fatty acids was established. The content of medium-chain fatty acids such as lauric and capric was decreased, while the amount of long-chain acids such as 11-hexadecanoic and stearic was increased in the experimental group. The main active components of AyuFertin - trienoic acids were not transferred into the milk of treated animals. In conclusion, due to the ability of AyuFertin to affect the fatty acids amount in the milk of treated buffaloes, farmers should strictly follow the manufacturer’s recommendation for the dose and duration of AyuFertin treatment to avoid negative effects on milk quality.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84640848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to evaluate the complications and the short-, medium-, and long-term outcomes following treatment of cranial cruciate ligament (CCL) rupture in dogs using the lateral fabello-tibial suture technique. The patients were ten dogs of various breeds, 62.5 ± 18.8 months of age, weighing 30.2 ± 3.3 kg, with unilateral CCL rupture. At 2, 6, 12, 24, and 52 weeks after surgery, the dogs were re-evaluated with clinical examination, gait and pain analysis, and radiography. A major complication was identified in one dog (10%), requiring second surgery due to implant failure and destabilisation of stifle joint. Minor complications occurred in two dogs postoperatively (one late meniscal injury and one case of seroma formation). The short-term outcome at two and six weeks postoperatively was considered good in six dogs and satisfactory in four dogs, while the mid-term outcome (between 12 and 24 weeks) was good to excellent in eight dogs and satisfactory in two dogs. All ten dogs had a good to excellent outcome 52 weeks after surgery. The lateral suture technique is generally safe and given the major benefits, such as lower costs, lower technical difficulty, and minimal requirement for specialised equipment, will probably remain a popular and alternative method for treatment of CCL insufficiency in dogs with good degree of owner satisfaction.
{"title":"Long-term clinical and goniometric follow-up of lateral suture surgery in dogs with cranial cruciate ligament rupture","authors":"R. Roydev","doi":"10.15547/bjvm.2021-0129","DOIUrl":"https://doi.org/10.15547/bjvm.2021-0129","url":null,"abstract":"The aim of this study was to evaluate the complications and the short-, medium-, and long-term outcomes following treatment of cranial cruciate ligament (CCL) rupture in dogs using the lateral fabello-tibial suture technique. The patients were ten dogs of various breeds, 62.5 ± 18.8 months of age, weighing 30.2 ± 3.3 kg, with unilateral CCL rupture. At 2, 6, 12, 24, and 52 weeks after surgery, the dogs were re-evaluated with clinical examination, gait and pain analysis, and radiography. A major complication was identified in one dog (10%), requiring second surgery due to implant failure and destabilisation of stifle joint. Minor complications occurred in two dogs postoperatively (one late meniscal injury and one case of seroma formation). The short-term outcome at two and six weeks postoperatively was considered good in six dogs and satisfactory in four dogs, while the mid-term outcome (between 12 and 24 weeks) was good to excellent in eight dogs and satisfactory in two dogs. All ten dogs had a good to excellent outcome 52 weeks after surgery. The lateral suture technique is generally safe and given the major benefits, such as lower costs, lower technical difficulty, and minimal requirement for specialised equipment, will probably remain a popular and alternative method for treatment of CCL insufficiency in dogs with good degree of owner satisfaction.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88252914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ectoparasitic infestations with Linognathus stenopsis and Bovicola caprae are a serious concern in goat farms, especially during the winter and spring. This study tested the efficacy of three modern insecticide drugs (ivermectin, eprinomectin and amitraz) applied via different routes for control of sucking and chewing lice in naturally co-infected goats. Each of drugs was tested on 30 goats. The animals from the first flock were treated with a single dose of ivermectin, applied subcutaneously at 0.2 mg/kg, the second one received a single spot on treatment with 1 mg/kg еprinomectin and the third flock was treated twice with amitraz, through spraying at a dose of 4 mL/1 L water. The results were evaluated before the treatments and at post treatment days 3, 14 and 60. On post treatment day 60, the application of ivermectin resulted in reduction of Bovicola caprae population by 73.05%, and for that of Linognathus stenopsis: by 92.70%. Eprinomectin demonstrated 100% reduction of both lice. The reduction of L. stenopsis and B. caprae caused by amitraz was by 92.20% and by 98.61% respectively. The macrocyclic lactone eprinomectin showed the highest efficacy (Р<0.05), was not toxic for goats and therefore, may be used as a treatment of choice for lice control.
{"title":"Tests on the efficacy of amitraz, ivermectin and eprinomectin for control of lice in goats","authors":"P. Prelezov, N. Nizamov, P. Veleva","doi":"10.15547/bjvm.2358","DOIUrl":"https://doi.org/10.15547/bjvm.2358","url":null,"abstract":"Ectoparasitic infestations with Linognathus stenopsis and Bovicola caprae are a serious concern in goat farms, especially during the winter and spring. This study tested the efficacy of three modern insecticide drugs (ivermectin, eprinomectin and amitraz) applied via different routes for control of sucking and chewing lice in naturally co-infected goats. Each of drugs was tested on 30 goats. The animals from the first flock were treated with a single dose of ivermectin, applied subcutaneously at 0.2 mg/kg, the second one received a single spot on treatment with 1 mg/kg еprinomectin and the third flock was treated twice with amitraz, through spraying at a dose of 4 mL/1 L water. The results were evaluated before the treatments and at post treatment days 3, 14 and 60. On post treatment day 60, the application of ivermectin resulted in reduction of Bovicola caprae population by 73.05%, and for that of Linognathus stenopsis: by 92.70%. Eprinomectin demonstrated 100% reduction of both lice. The reduction of L. stenopsis and B. caprae caused by amitraz was by 92.20% and by 98.61% respectively. The macrocyclic lactone eprinomectin showed the highest efficacy (Р<0.05), was not toxic for goats and therefore, may be used as a treatment of choice for lice control.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86439744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assessing the consumer’s awareness of the diet-health relationship, this study presents the fatty acid composition of cow’s milk available on Bulgarian market. Over a 5-year period, 100 samples of pasteurised cow’s milk and 135 samples cow’s yogurt were analysed by gas chromatography (ISO 15885:2002). The saturated fatty acids predominated - average amount 70.1% for milk and 71.1% for yogurt. The unsaturated fatty acids proportions were 29.9% and 28.9% respectively. Seasonal variations in the fatty acid composition of milk were detected, due to feeding practices. Non-milk fat was identified in 5 yogurt samples, while all pasteurised milk samples were not adulterated. This study provides the first scientific information for fatty acid composition of cow’s milk on Bulgarian market with respect to dietary intake estimation.
{"title":"Survey on fatty acid profiles of retail pasteurised cow’s milk and cow’s yogurt in Bulgaria","authors":"G. Kalinova","doi":"10.15547/bjvm.2294","DOIUrl":"https://doi.org/10.15547/bjvm.2294","url":null,"abstract":"Assessing the consumer’s awareness of the diet-health relationship, this study presents the fatty acid composition of cow’s milk available on Bulgarian market. Over a 5-year period, 100 samples of pasteurised cow’s milk and 135 samples cow’s yogurt were analysed by gas chromatography (ISO 15885:2002). The saturated fatty acids predominated - average amount 70.1% for milk and 71.1% for yogurt. The unsaturated fatty acids proportions were 29.9% and 28.9% respectively. Seasonal variations in the fatty acid composition of milk were detected, due to feeding practices. Non-milk fat was identified in 5 yogurt samples, while all pasteurised milk samples were not adulterated. This study provides the first scientific information for fatty acid composition of cow’s milk on Bulgarian market with respect to dietary intake estimation.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90047701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A cross-sectional study was conducted from April to June 2016 to determine the inhibitory effect of Lactobacillus strains isolated from buffalo milk and yogurt in Bandar-e Gaz, Golestan province, North-West Iran. The raw milk and yogurt samples were collected and cultivated on MRS medium by anaerobical incubation at 37 °C for 48 h. The suspected colonies were identified on the basis of Gram’s staining and conventional biochemical tests. The antibacterial activity of the cell-free supernatant extracted from Lactobacillus strains was determined using the agar well diffusion method against standard strains Escherichia coli ATCC 11303, Staphylococcus aureus ATCC 29213, Bacillus cereus ATCC 19115, Listeria monocytogenes ATCC 19111, E. coli and S. aureus isolates from local cheese samples. A total of 10 Lactobacillus spp. were isolated and identified as L. plantarum, L. casei, L. acidophilus. L. plantarum A1 and L. acidophilus R1 exhibited relatively strong inhibitory effect against S. aureus and B. cereus, respectively. Both isolates had no inhibitory effect against L. monocytogenes. L. casei B1 showed moderate inhibitory effects against L. monocytogenes. This study showed that Lactobacilli from buffalo dairy products had good inhibitory activity towards Gram-positive indicator organisms and were one of the best choices to control these pathogens in food products.
2016年4月至6月进行了一项横断面研究，以确定从伊朗西北部戈列斯坦省班达尔加兹市水牛奶和酸奶中分离的乳杆菌菌株的抑制作用。收集原料乳和酸奶样品，在MRS培养基上37℃厌氧培养48 h，通过革兰氏染色和常规生化试验鉴定可疑菌落。采用琼脂孔扩散法测定乳酸菌无细胞上清液对标准菌株大肠杆菌ATCC 11303、金黄色葡萄球菌ATCC 29213、蜡样芽孢杆菌ATCC 19115、单核增生李斯特菌ATCC 19111、大肠杆菌和金黄色葡萄球菌的抑菌活性。共分离到10株乳杆菌属，鉴定为植物乳杆菌、干酪乳杆菌、嗜酸乳杆菌。L. plantarum A1和L. acidophilus R1分别对金黄色葡萄球菌和蜡样芽孢杆菌表现出较强的抑制作用。两株菌株对单核增生乳杆菌均无抑制作用。干酪乳杆菌B1对单核增生乳杆菌有中等抑制作用。本研究表明，水牛乳制品乳酸菌对革兰氏阳性指示菌具有良好的抑制活性，是控制食品中革兰氏阳性指示菌的最佳选择之一。
{"title":"Antibacterial activity of lactobacilli from buffalo milk and yoghurt in Bandar-e Gaz, North-West Iran","authors":"F. Bagheri, A. Ahani Azari, H. Koohsari","doi":"10.15547/bjvm.2020-0073","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0073","url":null,"abstract":"A cross-sectional study was conducted from April to June 2016 to determine the inhibitory effect of Lactobacillus strains isolated from buffalo milk and yogurt in Bandar-e Gaz, Golestan province, North-West Iran. The raw milk and yogurt samples were collected and cultivated on MRS medium by anaerobical incubation at 37 °C for 48 h. The suspected colonies were identified on the basis of Gram’s staining and conventional biochemical tests. The antibacterial activity of the cell-free supernatant extracted from Lactobacillus strains was determined using the agar well diffusion method against standard strains Escherichia coli ATCC 11303, Staphylococcus aureus ATCC 29213, Bacillus cereus ATCC 19115, Listeria monocytogenes ATCC 19111, E. coli and S. aureus isolates from local cheese samples. A total of 10 Lactobacillus spp. were isolated and identified as L. plantarum, L. casei, L. acidophilus. L. plantarum A1 and L. acidophilus R1 exhibited relatively strong inhibitory effect against S. aureus and B. cereus, respectively. Both isolates had no inhibitory effect against L. monocytogenes. L. casei B1 showed moderate inhibitory effects against L. monocytogenes. This study showed that Lactobacilli from buffalo dairy products had good inhibitory activity towards Gram-positive indicator organisms and were one of the best choices to control these pathogens in food products.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78637976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recently, different reports highlighted the problem with dissemination of Escherichia coli producing extended spectrum beta-lactamases (ESBL) in poultry farms in Europe. The high incidence of Escherichia coli among poultry in Europe harbouring blaCTX-M-1 and the occurrence of ESBL and AmpC-producing Escherichia coli in raw meat samples collected from slaughterhouses in Europe have been discussed. ESBL-producing Enterobacteriaceae can be transmitted along the broiler production chain. Plasmids responsible for ESBL production frequently carry genes coding resistance to other antimicrobial classes, such as fluoroquinolones, aminoglycosides, sulphonamides. Resistance to cephalosporins in Enterobacteriaceae is of special concern for public health, because these antimicrobial agents are critically important. The aim of this mini review was to describe the mechanisms of resistance and prevalence of ESBL-producing E. coli. It is important to investigate the spread of these bacteria among poultry, the role of farm birds as reservoir of E. coli and the risk for people.
{"title":"Prevalence of poultry Escherichia coli isolates producing extended-spectrum beta-lactamases and their public health importance","authors":"R. Stefanova","doi":"10.15547/bjvm.2400","DOIUrl":"https://doi.org/10.15547/bjvm.2400","url":null,"abstract":"Recently, different reports highlighted the problem with dissemination of Escherichia coli producing extended spectrum beta-lactamases (ESBL) in poultry farms in Europe. The high incidence of Escherichia coli among poultry in Europe harbouring blaCTX-M-1 and the occurrence of ESBL and AmpC-producing Escherichia coli in raw meat samples collected from slaughterhouses in Europe have been discussed. ESBL-producing Enterobacteriaceae can be transmitted along the broiler production chain. Plasmids responsible for ESBL production frequently carry genes coding resistance to other antimicrobial classes, such as fluoroquinolones, aminoglycosides, sulphonamides. Resistance to cephalosporins in Enterobacteriaceae is of special concern for public health, because these antimicrobial agents are critically important. The aim of this mini review was to describe the mechanisms of resistance and prevalence of ESBL-producing E. coli. It is important to investigate the spread of these bacteria among poultry, the role of farm birds as reservoir of E. coli and the risk for people.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78144467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Famakinde, O. Mustapha, N. Okwelum, E. E. Teriba, M. Olude
Arhinia is a congenital nasal developmental anomaly that is seldom reported in literature, and especially not reported in domestic animals. This report describes a case of concurrent occurrence of congenital arhinia and choanal atresia in a day-old male kid which had no external nares with the nasal bones fused together with the nasal processes of the premaxillae presententing as a conical shaped rhinal structure with a tapering rostral apex and an occluded nasal vestibule. A bilateral osseous choanal atresia was also seen at the pharynx. Additionally, craniofacial and brain anomalies presented in this condition with right lateral deviation of the face and the absence of olfactory apparatus including olfactory bulbs, tracts and nerves and a vestigial trigonum olfactorium were noted. This, to the best of our knowledge is the first report in the literature indexed in the Medline of concurrent occurrence of congenital arhinia and choanal atresia in a goat.
{"title":"Concurrent arhinia and choanal atresia in a day old male kid","authors":"S. Famakinde, O. Mustapha, N. Okwelum, E. E. Teriba, M. Olude","doi":"10.15547/bjvm.2020-0031","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0031","url":null,"abstract":"Arhinia is a congenital nasal developmental anomaly that is seldom reported in literature, and especially not reported in domestic animals. This report describes a case of concurrent occurrence of congenital arhinia and choanal atresia in a day-old male kid which had no external nares with the nasal bones fused together with the nasal processes of the premaxillae presententing as a conical shaped rhinal structure with a tapering rostral apex and an occluded nasal vestibule. A bilateral osseous choanal atresia was also seen at the pharynx. Additionally, craniofacial and brain anomalies presented in this condition with right lateral deviation of the face and the absence of olfactory apparatus including olfactory bulbs, tracts and nerves and a vestigial trigonum olfactorium were noted. This, to the best of our knowledge is the first report in the literature indexed in the Medline of concurrent occurrence of congenital arhinia and choanal atresia in a goat.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84688407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Allahyari, M. Allymehr, A. Molouki, M. H. Fallah Mehrabadi, A. Talebi
Avian orthoavulavirus, commonly known as Newcastle disease virus (NDV) has been a constant threat for the poultry industry of Iran for decades. Recently, a couple of preliminary studies on backyard and commercial chicken suggested that a major subgenotype circulating in Iran may be VII(L) subgenotype, which is now known as VII.1.1 according to the new classification system. The unique subgenotype was not reported from other parts of the world and was slightly (≥3%) different from the closest group that was VIId. The study was conducted between July 2018 and March 2019 to determine the exact NDV genotypes/subgenotypes circulating in Iranian broiler poultry farms; five-hundred and forty chickens were sampled from thirty-six broiler farms located in eighteen provinces of Iran. As other genotypes/subgenotypes such as XIII and VI.2 are circulating in neighbouring countries, border provinces were also sampled. The F gene of the NDV isolates was sequenced and phylogenetic analysis was conducted. All the isolates clustered under the VII.1.1 group. The evolutionary analysis also revealed that the distances were between 0.0 and 0.7% meaning that the Iranian NDV circulating in broiler farms were not only of VII.1.1 sub-genotype, but also genetically very identical, indicating that the routine control measures for ND in Iran were not able to prevent the circulating NDVs. Although stricter biosecurity measures have been really effective in developed countries, surveillance of NDV to determine the circulating genotypes might also help us to implement better vaccination strategies in the future.
{"title":"Molecular characterisation and phylogenetic study of the fusion gene of Newcastle disease viruses isolated from broiler farms of Iran in 2018-2019","authors":"E. Allahyari, M. Allymehr, A. Molouki, M. H. Fallah Mehrabadi, A. Talebi","doi":"10.15547/bjvm.2020-0041","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0041","url":null,"abstract":"Avian orthoavulavirus, commonly known as Newcastle disease virus (NDV) has been a constant threat for the poultry industry of Iran for decades. Recently, a couple of preliminary studies on backyard and commercial chicken suggested that a major subgenotype circulating in Iran may be VII(L) subgenotype, which is now known as VII.1.1 according to the new classification system. The unique subgenotype was not reported from other parts of the world and was slightly (≥3%) different from the closest group that was VIId. The study was conducted between July 2018 and March 2019 to determine the exact NDV genotypes/subgenotypes circulating in Iranian broiler poultry farms; five-hundred and forty chickens were sampled from thirty-six broiler farms located in eighteen provinces of Iran. As other genotypes/subgenotypes such as XIII and VI.2 are circulating in neighbouring countries, border provinces were also sampled. The F gene of the NDV isolates was sequenced and phylogenetic analysis was conducted. All the isolates clustered under the VII.1.1 group. The evolutionary analysis also revealed that the distances were between 0.0 and 0.7% meaning that the Iranian NDV circulating in broiler farms were not only of VII.1.1 sub-genotype, but also genetically very identical, indicating that the routine control measures for ND in Iran were not able to prevent the circulating NDVs. Although stricter biosecurity measures have been really effective in developed countries, surveillance of NDV to determine the circulating genotypes might also help us to implement better vaccination strategies in the future.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"103 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86031796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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